LOCUS       NZ_CP095509             7459 bp    DNA     linear   CON 20-JAN-2024
DEFINITION  Escherichia coli strain GN02050 plasmid p2050-6.
ACCESSION   NZ_CP095509
VERSION     NZ_CP095509.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27361453
            Assembly: GCF_026651085.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7459)
  AUTHORS   Bethke,J.H., Ma,H.R., Tsoi,R., Cheng,L., Xiao,M. and You,L.
  TITLE     Vertical and horizontal gene transfer tradeoffs direct plasmid
            fitness
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7459)
  AUTHORS   Bethke,J.H., Ma,H.R., Tsoi,R., Cheng,L., Xiao,M. and You,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2022) Molecular Genetics and Microbiology, Duke
            University, CIEMAS Building 101 Science Drive, Durham, NC 27705, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 6.0.0.47841
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 508x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/20/2024 04:55:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,269
            CDSs (total)                      :: 5,143
            Genes (coding)                    :: 4,917
            CDSs (with protein)               :: 4,917
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 89 of 226
            Pseudo Genes (incomplete)         :: 142 of 226
            Pseudo Genes (internal stop)      :: 46 of 226
            Pseudo Genes (multiple problems)  :: 46 of 226
            Pseudo Genes (short protein)      :: 3 of 226
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095509.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="GN02050"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2050-6"
                     /geo_loc_name="USA"
                     /lat_lon="36 N 78.9 W"
                     /collection_date="2002"
                     /collected_by="Vance G. Fowler Jr."
                     /genotype="144"
     gene            <1..320
                     /locus_tag="MW398_RS26155"
                     /old_locus_tag="MW398_26160"
     CDS             <1..320
                     /locus_tag="MW398_RS26155"
                     /old_locus_tag="MW398_26160"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_268091497.1"
                     /translation="YVEEFAFLFCESCFVVEQVNNLSLSCGLFSELPDESDPFLQPFDV
                     ADAHVRGAPWCFLLCVVVVQYIACGRWVQGVSSVLIRKIRRKRTYMFLSFVEKQCAPAE
                     G"
                     /name="hypothetical protein"
     gene            325..1251
                     /locus_tag="MW398_RS26160"
                     /old_locus_tag="MW398_26165"
     CDS             325..1251
                     /locus_tag="MW398_RS26160"
                     /old_locus_tag="MW398_26165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_023144241.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPDRGGAKKDSGNLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYYRHLEQARMVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDGVSRENRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1254..>1780
                     /locus_tag="MW398_RS26165"
                     /old_locus_tag="MW398_26170"
                     /pseudo=""
     CDS             1254..>1780
                     /locus_tag="MW398_RS26165"
                     /old_locus_tag="MW398_26170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(1842..2064)
                     /locus_tag="MW398_RS26170"
                     /old_locus_tag="MW398_26175"
                     /pseudo=""
     CDS             complement(1842..2064)
                     /locus_tag="MW398_RS26170"
                     /old_locus_tag="MW398_26175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(<2631..2922)
                     /locus_tag="MW398_RS26175"
                     /old_locus_tag="MW398_26180"
                     /pseudo=""
     CDS             complement(<2631..2922)
                     /locus_tag="MW398_RS26175"
                     /old_locus_tag="MW398_26180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855398.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            3081..4782
                     /locus_tag="MW398_RS26180"
                     /old_locus_tag="MW398_26185"
                     /pseudo=""
     CDS             3081..4782
                     /locus_tag="MW398_RS26180"
                     /old_locus_tag="MW398_26185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007372656.1"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /name="FAD-dependent oxidoreductase"
     gene            6093..7006
                     /locus_tag="MW398_RS26185"
                     /old_locus_tag="MW398_26190"
                     /pseudo=""
     CDS             6093..7006
                     /locus_tag="MW398_RS26185"
                     /old_locus_tag="MW398_26190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(7210..>7459)
                     /locus_tag="MW398_RS26190"
                     /old_locus_tag="MW398_26195"
                     /pseudo=""
     CDS             complement(7210..>7459)
                     /locus_tag="MW398_RS26190"
                     /old_locus_tag="MW398_26195"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /pseudo=""
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     Typing          6094..6868
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.521"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          326..1248
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agtacgtgga ggagtttgcg ttcctgttct gtgagtcctg cttcgttgtc gagcaggtca
       61 ataatctgtc tctgtcgtgc ggtctgttct ccgagcttcc tgatgaatcc gatccgtttc
      121 ttcagccatt cgatgttgct gatgcgcatg ttcgtggtgc tccctggtgt tttttgctgt
      181 gtgttgtggt cgtgcagtat atcgcttgcg gtcggtgggt gcagggggta tcttccgtgc
      241 taattcgtaa aattagaagg aagcgcactt atatgtttct ctccttcgtc gagaaacagt
      301 gcgctcctgc agagggctga aaccgtggca acctatcacc tcagcgttaa atttggtggc
      361 aaggggcagg ctgcaaacca cgccgattac atcgagcgca aagagaaata ccgcgaccgt
      421 caggatttgg aatattccgc acacggaaac atgccggaat gggccaggga taatccttcc
      481 catttctggc aggcggctga ccagtttgaa cgtgcgaacg gttcgaccta tagggaactt
      541 gaaattgcat taccccgtga actgacaccg gaacagcgcc ttgaactggt gcaggatttt
      601 gtacgtcagg aagctggcga acggcacgcc tggagttttg ccattcacaa tccgaaagcg
      661 agcattgacg gcggcgaaca gcctcatgcg catatcatga tgagtcagcg tgtgaatgat
      721 ggcattgaac gtacaccgga acagtatttc cggcgctata acgccaggta tcccgaccgg
      781 ggaggtgcga aaaaggattc cggaaacctg acccccactc agcaaaaaga gcagttgcgg
      841 gaattgcgaa aacgctggga ggtgaagcac aacgaacata tgagaaagca tggctttgaa
      901 aggggcttca ttgactgccg gacactgaaa gaacagggca ttgaacgacg tcccgaagtg
      961 catctgggtt ttgaagctgc cggacgactt gatgatgccc agcgtcatga catcatgcaa
     1021 aaacggtctg aaccggacta ttaccgccat cttgaacagg cgcgaatggt cgggcatgac
     1081 atcagggaat ggctggatac accggttaag caacatgaac tggtcagaag gctgcaacat
     1141 gatgttgcgc gggagcatgg ctttgacatg agagacggag tgagcagaga gaaccgaact
     1201 gaacctgaac gggttaaatc accgtcacgt ggattttcta tgggattctg aacatggcca
     1261 gcacagaaga aaaattactc acgctcctgt tatcggtgga gcatctgcaa acataccgca
     1321 atagagcaac agaaagctgt tgctgattct ctggctgtgg caagggaaac tcttcgcgga
     1381 tatcgggata ttctgacggt gtggcaaaac aggtcaggga tgaagtcaga acgaggtgca
     1441 gaagctggac gtggcaggac tgataggtga gcgcatcagc caggattttc agcggctgga
     1501 agagtcagtc gcaaacatga aggcaaacgc ggatgccctg aacagggata ttgcgacaat
     1561 tcacagcaat atcgtgcagg aatacaacac ccttaaaggg tatagctgga aatggctggg
     1621 tggggtgtca gtggcttttc tggttatgtt ctgtgtgctc ggatggggaa tgaaaacaat
     1681 actcgacagg aactacaacg ccacagcggc ggtgtatcag aaagtggtga cactggaaga
     1741 taaactggtg gagaaacctg tcacggggaa gagccgggaa aaactgacgc gcttcgcttc
     1801 gtccaacgcc tcacggagcg cgaaaggatt tcgcgaaccg ttcaccgttt tttccaatat
     1861 gattctgtct gctttggtcg ataaagccgt cgatagtatt ccagccttcg tatatataac
     1921 ggtacgtgct catcagtaag atctggcggt attgtgttat cagggggaag ttttttcaga
     1981 tactcacgaa atgcctggct ttgatgccat aaaatctaca gctgcatcga taatatgtgg
     2041 gatattttca tctaattttg tcatgatgat tgatctgata ttatcacggt tcgtgaatta
     2101 tagtatgtca ctattattta tgcgttagtg aaccagaaaa aatccccccg ttggtatagg
     2161 gggatatgta aggagaaata acttcgcagg ttgttcttgt ttgtattata acgtgaaaga
     2221 atgcacgcaa cttaattaat ctgccgtact tcctttcagt tttcaagata aggtttataa
     2281 taaaacatgg aatggaagca agctttaagg tttcagctct aaaattttta atatctatta
     2341 actagctagt tgttgtcctt gtgagcatgt tgctatataa aagctaggat aatatcctga
     2401 ccggagtatt catattcctt atcccctctg tgtttctcag aggttttttt ctgatttaat
     2461 gatatgagct ggaatgcgat tccggccccc ccgtttacat aattctcccc tgacatacgg
     2521 caccggaatc gcatgatttt gcattgttaa tgtctaactg tgctgtgtga atgttggggg
     2581 cagtttggat atcgaaaatt attgtacgtt aaggttgttt ttttgacctt gtcggggtcc
     2641 gcctgcgggc agtagagatc atataaggta ttcaataacg tcatgatgcg gttatccccg
     2701 atacggtaat ataccatttt acctgcccgt cgggtctcga ccagcttcag gcgacgcatg
     2761 acacccaact gctgagacag ggtcggctgc agtgataccg accgccgctt ccagctcggt
     2821 cacattcgct tccccctggc ttaactgaca catcaacaat agccggtcgc tgttggccag
     2881 tccccgtagg acatctgctg cccccagggc tgcatttttc atatcagtct gacgttttgt
     2941 gtcttcattc tatgggcatt gctggtttct tatattatgt atttttatat aatattgttc
     3001 agtataatga tagtggcgta attttgccac cgtgatcttc aggtctgcag cgtccagctg
     3061 gaccgggtaa ctaattatat atggcagata ttttgccccg agtgaacaga gaggattgta
     3121 atgaaatagt gattgttggt ggtgttgccg ggggagcatc cgctgcagcc agggctcgtc
     3181 ggttatcaga ggatgtcagt attgttgtgt ttgagcgggg aagcgatgtc tctttcgcca
     3241 actgtggatt gccttatcat ataggcggaa aaattccgtt acggcagtcg ctgattctga
     3301 aaaccccgga ggattttaaa tcccgtttta acatagacgt ccgtatccgt catgaagtga
     3361 tgtcagtaga cccggttaat aagatggtga cggttaaaaa tctgacctcc ggggaggtct
     3421 ataccgaaga atgggatcgt ctgctcctca gtaccggtgc cgctccggtt gttcccccct
     3481 tgcccggact acaggaagcg ggtgtcttta cactgcgaaa tcttactgac atggatgcta
     3541 tccaggcgtg gattgagcaa cataatatcg ctcacaccac gcttgtcggg gggggattta
     3601 tcgggctcga agtgatggag gcactgagcg aaagggggat cagtgtgacc ctgctggaga
     3661 tgggggaaca ggttatggct ccggttgatc ctgaaatggc atcggccctg catcaggaaa
     3721 tcaggagtca tggcgtggat ctgcgtctca gaacggcgct caccgaggta ctgcggacag
     3781 agacgggatt ccgcgttgca ctgtctgacg gtgggtttct gcagaccgac atggttattc
     3841 tcgccatcgg ggtaaaacct gagagcagcc ttgccacagg agccgggctc gccgtgggta
     3901 aacggggagg cataagtgtc aatgcctgta tgcagacaag tatccctgac atctatgccg
     3961 tggggggatg cagttgaaac acctgacctt gtctttcagg aacccgctaa tgttcctctg
     4021 gcagggccag ccaaccgcca gggacgcatt gctgctgata atatgctcga tcgtcacagc
     4081 ctttaccatg gcagtcaggg gacatccatt tgtaaggtat tttcgctgag tattggcagt
     4141 gtcggggcga atgaaaagca gctgaaagcc catggtaccc gatacgagaa ggtttacgtc
     4201 catgccgctg atcatgccag ttactacccg ggggcaacaa tgatcagtct gaaactgctg
     4261 ttcagccccg ataccggtga aattctcggg gcccaggcgt cagggaaaaa aggggttgat
     4321 aaacgcatcg atgtcctggc tgtcgtacag cgcgcggggc ttaccgttaa cgatctcgag
     4381 catctggagc tgacctatgc tccgcctttc aacagtgcaa gggacgtcgt taaccaggct
     4441 ggaatggtgg cggcaaatgt tatcaagggc gatacggtca tctgtcatgt ccgcgatgtg
     4501 ttacaacggg aaccgggcgg cagttattgt ttacttgata ttcgttctcc tgcagaactg
     4561 aaacaatttg gtgaatatcc tgacgcgcta tccatccctc ttgattccct gcgggagaat
     4621 cttgataagt tgcctgaaga taaagagatt attatcgggt gtcagagtgg tctgcggggg
     4681 catgtggcat accgtatgct tcagtctcac ggcttccgga catataatct gagtggtggc
     4741 tttataactt ggcaggcggt tacggaatca atgagtcaat aagagatgag gtgccataca
     4801 catggcacct catcttccag attcattgaa aacgtattat atggaataga aatctacaaa
     4861 aataattaac accctggatg gtaccctatt gcgataatgt ggactgttca aaaaacaggc
     4921 ttaccgtaca ataattctct atatccaaac tgaccccaat gttcctgaat gtaaatctcc
     4981 gtacaaagat atcgttgagt tttatataat tcattgtttt tattgtgttt tgtttttttg
     5041 gttgtgctgg tttgtagtta taaaaaaccc ctgggaatcc aggggtaggt aaggaaaaca
     5101 aatgatactc agttagtgtt gcactttaaa cgggttgaag tataactcgt attaaatgag
     5161 atgttgaaaa taagtgaaaa ccattgctgg ttgcaatagg gcagtatgcg taaaaaataa
     5221 atttgtgatg cttttcaaaa aacaattaat acatgaatat tacagctcat atattacagt
     5281 ttataaaaaa actgtgtttt atctattaca tagagaaaaa ttcttatcct gagctgtgaa
     5341 cttggttgtt tcgtttgata tatatctttg taaatgtggt ttattatgcg ggcatttttt
     5401 attgagtatt gaatcagaat aagtgatgca gctaaattta tttgcgtaac tatttcattg
     5461 tcaatattct gactcaatct gacacctatc gtttctcttt gattccccac ttttgtgttt
     5521 tttttatccg gaacgggata gtgccagttg ttttttatca gcctcagggt attttggggc
     5581 ggttgcgaac attgacgcat tccacaacta aaaaaaattt ctcacagact ctgattcttg
     5641 ccccgaccct tggtcggttt ttttttgtcc tggcgaaacg tgaagctctg aaacagaaaa
     5701 tctccgggca gaaccttcca tccgggggag aaaacttttc gttttgagcc gttcggtgtt
     5761 cagaacgcac gaaaccgatc gcacatcgct ttcgtgcata gttatgcaac gccctgaaaa
     5821 cgattctgac gcgtttttct ggttttgcct ggtgttttct tgtctttttg cgttttttgc
     5881 gccagaaagc gactgagggc gttttaaggg gggcgtacaa cgggagttat ggtaaatgga
     5941 tcggtttttc gggaaggatc gacaggattt gccgttgggt gtagtgtaag cgactgaaaa
     6001 acaaacgccc ctgaaattcg tgtctctccg ccaagattgt cacgagaata caggggcgag
     6061 gtcggtcgat aacctggaag gaatcgtaac acatgagcgc cgcgcttcaa tacttcgaag
     6121 aaaatttacc cctccgcccc tatcacacgg atgatctcgc tttcggactt cgtatctccg
     6181 gtaaagggcg tgcgcttctt gcacggtata tccagcagaa ccagcctcat gcacagttct
     6241 ggctggtttt tgacgttgac cgcgaggggg ctgcgattga ctggagcgac cggaacgccc
     6301 ccgcgccgaa catcaccgtt aaaaatcctg tgaacggaca cgctcacctg ctctacgcac
     6361 tcaacatcgc cgtgagaacc gcgcctgatg cctcggttaa ggcgctgaaa tatgccgcag
     6421 cggttgaacg ttcgctgtgt gagaaaattt gtgctgatgt gaactacagc ggcctgattt
     6481 gcaaaaatcc gttccacctg gaatggcagg tgatggagtg gcgtgaggaa gcctatacac
     6541 tcgatgaact ggctgattat ctcgatttga gcgcctcagc gcgtcgtagc atcgataaac
     6601 attacgggat ggggcgaaac tgccacctgt tcgaaatgac gcgcaaatgg gcttacaggg
     6661 cgattcgtca gggatggcca gcattctcac agtggcttga tgcggtgatt cagcgtgtcg
     6721 aaatgtacaa tgcatcgctt cccgttccgc tttcaccgcc tgaatgtcgg gctattggca
     6781 agagtattgc gaaatacacg cacaggaact tcacgccgga aactttcgca cagtatgtgg
     6841 ctgatacgca cacaccagaa attcaggctg cacgcgtcgc aagggcggtt ctaagtctaa
     6901 gcgcagcaca gtagctacat ctgcacgcac gctgaaaccg tgggaggcat tagggatcag
     6961 tcgcgcctgg tattaccaac tgaaaaaacg aggtctcgta gagtagacca aataagccta
     7021 tatcagataa cagcgccttt ctggcgtctt tttgagcagc aggttttttg ccgtttttcc
     7081 ccgttcagcc ctgcaatggc gcggctttcc gcgtgattgt ggtggtagtg ctcgccgcag
     7141 tctcatgacc gagcgtagcg agcgaatgag cgaggaagca ccagggaatc ggaacacgga
     7201 tggaggcact tacgcgtctg ggtttgggga tgtctccaga tcgtacttaa gttccttaag
     7261 tgcttgtaat atcctgtctt ttttatagca tagctcgcct gtttttgtgt ctaccagtcc
     7321 tgcttcgatc agtagcccag ccgcgagaaa gagccggtga ttgcgttccc gtcgttgttt
     7381 ttttccctcg attcgttttt ggaccgcctg ttttcgttcg ctctcctgtg cctgtaagtc
     7441 gtttttttcc gctgttgcc
//
