LOCUS       NZ_CP095508             5632 bp    DNA     linear   CON 20-JAN-2024
DEFINITION  Escherichia coli strain GN02050 plasmid p2050-7.
ACCESSION   NZ_CP095508
VERSION     NZ_CP095508.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27361453
            Assembly: GCF_026651085.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Bethke,J.H., Ma,H.R., Tsoi,R., Cheng,L., Xiao,M. and You,L.
  TITLE     Vertical and horizontal gene transfer tradeoffs direct plasmid
            fitness
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5632)
  AUTHORS   Bethke,J.H., Ma,H.R., Tsoi,R., Cheng,L., Xiao,M. and You,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2022) Molecular Genetics and Microbiology, Duke
            University, CIEMAS Building 101 Science Drive, Durham, NC 27705, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 6.0.0.47841
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 508x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/20/2024 04:55:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,269
            CDSs (total)                      :: 5,143
            Genes (coding)                    :: 4,917
            CDSs (with protein)               :: 4,917
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 89 of 226
            Pseudo Genes (incomplete)         :: 142 of 226
            Pseudo Genes (internal stop)      :: 46 of 226
            Pseudo Genes (multiple problems)  :: 46 of 226
            Pseudo Genes (short protein)      :: 3 of 226
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095508.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="GN02050"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2050-7"
                     /geo_loc_name="USA"
                     /lat_lon="36 N 78.9 W"
                     /collection_date="2002"
                     /collected_by="Vance G. Fowler Jr."
                     /genotype="144"
     gene            complement(<4..735)
                     /locus_tag="MW398_RS26120"
                     /old_locus_tag="MW398_26125"
                     /pseudo=""
     CDS             complement(<4..735)
                     /locus_tag="MW398_RS26120"
                     /old_locus_tag="MW398_26125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(725..1048)
                     /locus_tag="MW398_RS26125"
                     /old_locus_tag="MW398_26130"
     CDS             complement(725..1048)
                     /locus_tag="MW398_RS26125"
                     /old_locus_tag="MW398_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1113..1304
                     /locus_tag="MW398_RS26130"
                     /old_locus_tag="MW398_26135"
     CDS             1113..1304
                     /locus_tag="MW398_RS26130"
                     /old_locus_tag="MW398_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165975.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2507..3373)
                     /locus_tag="MW398_RS26135"
                     /old_locus_tag="MW398_26140"
     CDS             complement(2507..3373)
                     /locus_tag="MW398_RS26135"
                     /old_locus_tag="MW398_26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(3375..4427)
                     /locus_tag="MW398_RS26140"
                     /old_locus_tag="MW398_26145"
     CDS             complement(3375..4427)
                     /locus_tag="MW398_RS26140"
                     /old_locus_tag="MW398_26145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_059342428.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPRELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            complement(4908..5141)
                     /locus_tag="MW398_RS26145"
                     /old_locus_tag="MW398_26150"
     CDS             complement(4908..5141)
                     /locus_tag="MW398_RS26145"
                     /old_locus_tag="MW398_26150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137437.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5148..>5632)
                     /locus_tag="MW398_RS26150"
                     /old_locus_tag="MW398_26155"
     CDS             complement(5148..>5632)
                     /locus_tag="MW398_RS26150"
                     /old_locus_tag="MW398_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_268091496.1"
                     /translation="ERAESTEKMLNDEFRKLEESVNRELTSNESLIRNAINDHTTALKE
                     LLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTGSLLWYQGMKINANMNI
                     LREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTKDNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     Typing          complement(1399..1480)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(1937..2124)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          complement(5..735)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.59"
                     /coverage="99.0"
                     /evalue="4.27e-160"
     Typing          complement(4805..5631)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="3.2e-157"
ORIGIN
        1 tcagcttttt tctcgaactc cgcgcccgtc tgtaaaagat tgctcataaa acgctccttt
       61 cagtctgata ttcctcccgc cgttcgggtc tgcaatgctg atactgcttc gcgtcaccct
      121 gaccacttca agccccgcct ctgtgagcgc ctgaatcaca tcctgacggc cttttatctc
      181 tccggcatgg taaagcgcgt ctatcccgcg tgtgacgccc tcagcaagcg cctgtttcgt
      241 ttccggcagg ttatcaggaa gggtcagcgt ccggcggttc tccggggcgt tggggtcatg
      301 cagaccgtaa tggtgattca ccagcgtctg ccaggcatca attctcggcc tgtctgcccg
      361 gtcgtagtac ggctggaggc gttttccggt ctgtagctcc atgttcggaa tgacaaaatt
      421 cagctcaagc cgtcccttgt cctggtgctc cacccacagg atgctgtact gatttttctc
      481 gagaccgggc atcagtacac gctcaaagct cgccatcact ttttcacgcc ctccaggcgg
      541 cagctccttc tccgcgaacg acagaacacc tgacgtgtat ttcttcgcaa atggcgtggc
      601 atcgatgagt tcccggacct cttccggatt cccctgaagc accgttgccc cttcccggtt
      661 acgctccctt cccagcaggt aatcaaccgg accactgcca ccaccttttc ccctggcatg
      721 aaacttaact atcatcccgc gccccctgtt ccctgaccgc cagacgcagc cggcgcagct
      781 catccccgat ggccatcagt gcggccacca cctgaacccg gtcaccggaa gaccactgcc
      841 cgctgttcac cttcctcgct gtctggttaa ggttattccc gatggcggcc agctgacgca
      901 gtaacggcgg tgccagtgtc ggcagcttcc cggaacgggc aaccggctca cccaggcaga
      961 cccgccgcat ccataccgcc agttgtttac cctcacagcg ttccagtaac cgggcatgtt
     1021 catcatcagt aacccgtatc gtgagcatcc tctcgcgttt catcggtatc attaccccca
     1081 tgaacagaac ccccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta
     1141 acatggcccg cttcatcaga ggccagacat taacgctgct ggagaagctc aacgaactgg
     1201 acgcggatga acaggcagat atctgtgaat cgcttcacga ccacgccgat gagctttacc
     1261 gcagttgcct cgcacgcttc ggggatgacg gtgaaaaccg ctgacacatg cagctcccgg
     1321 agacggtcac agcttgtctg tgagcggatg ccgggagcag acaagcccgc cagggcgcgt
     1381 caggccgttg acctttgggg tgtcggggcg aagccctgac ccagtcacgt agcgatagcg
     1441 gagtgtatac tggcttaacc attcggcatc agtgcggatt gtatgaaaag tgcgccatgt
     1501 ccggtgtgaa atgccgcaca gatgcgtaag gagaaaatgc cagttcaggc gctcttccgc
     1561 ttcctcgctc actgactcgc tacgctcggt cgttcggctg cggcgagcgg actctccttt
     1621 ctcaaaaacg ctaaccctgt tatccacaga acccggggat aaaccaggaa agaacatgtg
     1681 cgcaaaaaac gaaaccccgg gaaaggccgc gccggaggcg ctttttccat aggctccgcc
     1741 cccctgacgg acttcacaaa acagacactt tttcggtgca tctgtgggag ccttgcggct
     1801 caaccatgaa atccgccagc ggtggcgaaa cccgacgtgg cttaaagatc atcaccgttt
     1861 ccggtgaatc gctccctcct gcgcccccac gttcccgcca tatcgattac cggatacctt
     1921 ttccgccttt ctcccttacg ggacacgtgg cgctttctca tagctcacgt cttctggtat
     1981 ctcggggcga cttaggcagt tcgctccggg ctgggctgta tgcaggaacc ccccgttcag
     2041 cccgactgct gcgccttctc cggtaactgt tctcttgggt ccaacccgga aaagacacca
     2101 caaagcgcca ctggcagcag ccattggtaa aagtcatgtg cgagatattt agagagtact
     2161 tgaagtggct gccggagtgc ggctacacta gaagaacaat tttggtgact cgtctcgcat
     2221 aagacagtta cctggttaag cagtctgcaa gctgacttaa ccttcgatca aaccgccctg
     2281 caaggcggtt ttttcgttta caggcagcag attacgcgca gaaaaaaagg atctcaagag
     2341 gatcctttga tctttttgtt acaaacgtct ctatttgact cccctgagag cgttttagcg
     2401 cgcctctgac ccccttggtt acattccgac accagaaacg ctcccgtttt ttacgtgcat
     2461 ttggcacggt atcgatggag gccggggggc aatcctgaac agaatgttac tcctctgagt
     2521 attttctgtt aagaaaatat acacttagca cagttacaat aaatcctacc ccctgtaaga
     2581 atgtgaattg ttcatccaaa aacaaccatg acaaaaataa tgcaactagc ggaataaagt
     2641 acaacattat ccctgccaaa tgaactggga gccaacgcaa ggagttcaag taaaaacaaa
     2701 aagctaaaaa atattgtaat attccagatg aaaaaacaat aatcacggat tccaacggaa
     2761 gataaattga tgatttagat ataaaaataa agagcgatga cagtataagg gagaatgtca
     2821 actgtccgct caataatgtg ataggctcaa acgatttaat aaggcgagac gatataacaa
     2881 cataaaatgc tgccgataac acaccggcaa caacaagaca atatccaaca atattattcg
     2941 atccattact gatgtctggc aaggctacca aaatactgcc aacaattgca cctataagga
     3001 acaatataaa atattgcggg gaattaattt taactgagca aataagaaca gacaacacca
     3061 atataaacac tgattcaagt gaaaaaataa ttgatgcgct tcctgctgaa ataaaataaa
     3121 gtccgtagag tgtaaaagta tacgctaaga atggttcaaa aacgcctgtc gcataagcaa
     3181 gtatcgcttt tgagtttaat gggagttttt ttctttttat aaaaagaact atccaagata
     3241 gtattactgc actacaaatc tgccagaaaa acacccctcc ggcatctagt tcttcaactc
     3301 cttttttgct cattacgata gctgcccccc atgagaacgc tgctaaaaaa gcaaaaagca
     3361 ttcctttaat catattaatc atcctctttc tcataaaggg gtttgttttc aaatttcctt
     3421 ggttgaaagg agttaaaaaa catagatgca tcatctacag gaataccaat aaactctcca
     3481 taataaggat catctatagg aaactcttct aatgaaacat aaaacaatac attccagttg
     3541 aagatcattg gatcactatt ttctatttca tggagcaatc tttcatcctt agttaattct
     3601 cttggaatta atacatcagt atgataaaaa ccgctgtaat aagaactatt accggtgtgc
     3661 actgggcatc caaaacgatc caaattagat tctgaatgcc atgttgtaga aatcccttcc
     3721 ggatgtgaat acccaccctt accaacagac tgaatagtca tgttacgaat atccgctaac
     3781 gcttgttctt gatctttata gaaacctttt tctatcgcca gcctcataaa atgccccggg
     3841 gcatttacag aaataagtat tccccctggt attcttctat catgatttct tgttctatga
     3901 gcagaagagt aaaaaacatt aacaccagcc ttatatattt ttagcccatc tttatcctga
     3961 aatggtacgc tttcagtata aataacatcc tcttttattg gatagcactc tggcatatgc
     4021 agcgaacaaa tatatttttg tatgtccact aactcttctg atggacgagc aaaggcaagt
     4081 tgtggatgaa taaaataaat gagatgcgca ctgacctctc ctcttcttgc gcgttcttta
     4141 aaacgaactc tttcattacg caaaaaatca aacaaatgac catcatcatt atatagttgg
     4201 gggctattta caatagataa atggaaatca agccctttaa ttttcttgct tgccaattta
     4261 ccaaatacac agggctgatt gcctgacacc cattttctta gtaaatcttc aattttatct
     4321 ttagaagttt cgttaaagat aaatgaatga atattatcaa tatcttttcc aaatgaaaaa
     4381 ctggatgata attgtggtag tttatttata cgttgttgaa gattcatgaa agctcccaca
     4441 agacacctca tatatttttt tatatactga aactacatta tatttattac cggcgtaaaa
     4501 cgaaataatt tattatcgtt accgatattt taaaaaaatc attatttgca cagtgttcga
     4561 gttgttagta tcactccttc ctgaaaatcc gctccattta ccataacgtc gcattgtacg
     4621 caccgccaaa acgcgttcgg tgcgatcacg gcagcagaca ggtaaaaatg gcaacaaacc
     4681 acccgaaaaa ctgccgcgat cgcacctgat aaattttaac cgcatgaata cctatgcaac
     4741 cagatgctac aggccacatt agcccgcctt aatccactga acacgtcatt tttcatggtt
     4801 tcaccatccc agcgaagggc catccagtgt ccgctcctgg atttccggct gacgctcccg
     4861 ctccagagat aactcatgtt cgcgttcctg catcagccgt tcatccctta tctcctgttc
     4921 tcgctgtata actggctcaa gcgttctgtc cgctcgctca agtgctgcac ctgctgactc
     4981 aactgcatga ctcgcccgtt cagcatcgcg ttgtcccgtt gcgtaagcga aaacattttc
     5041 tgcaattcca cgaaggcgtt ctcccattcg ttcagccgct gcatatagtc ctgttgcagt
     5101 tgctctaatg cgttcagcaa atgtctttcc agctccgtca tgctctgtca ctcctgcacc
     5161 agtctcactg cgttcagttt gccgttgtcc ttcgtccagt tcgtttcggc tttcatcccc
     5221 ttcggcaaca ccagaaaacg tccgttgctg tcctcgtgat aacgcacgcc ccatgtcttc
     5281 gcgttcagct tctccagact ctctttctgc tcgcggagaa tattcatatt cgcgtttatc
     5341 ttcatgccct ggtaccacag cagactcccg gtcgtggcaa acatcagtac tgagacgata
     5401 atcagccaca gccagcgttt cacgctcatt ttcagtacgg ttttccagtg cgcgtccatc
     5461 gtatcgacgg tcgttttctg atacctctcc agcagttctt tcagggctgt tgtatggtcg
     5521 ttgatagcgt tcctgattaa agattcgttt gaggtcagtt ccctgtttac agattcttcg
     5581 agcttcctga actcgtcgtt cagcattttc tctgtagatt cggctctctc tt
//
