LOCUS       NZ_CP095507            10934 bp    DNA     linear   CON 20-JAN-2024
DEFINITION  Escherichia coli strain GN02050 plasmid p2050-4.
ACCESSION   NZ_CP095507
VERSION     NZ_CP095507.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27361453
            Assembly: GCF_026651085.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10934)
  AUTHORS   Bethke,J.H., Ma,H.R., Tsoi,R., Cheng,L., Xiao,M. and You,L.
  TITLE     Vertical and horizontal gene transfer tradeoffs direct plasmid
            fitness
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10934)
  AUTHORS   Bethke,J.H., Ma,H.R., Tsoi,R., Cheng,L., Xiao,M. and You,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2022) Molecular Genetics and Microbiology, Duke
            University, CIEMAS Building 101 Science Drive, Durham, NC 27705, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 6.0.0.47841
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 508x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/20/2024 04:55:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,269
            CDSs (total)                      :: 5,143
            Genes (coding)                    :: 4,917
            CDSs (with protein)               :: 4,917
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 89 of 226
            Pseudo Genes (incomplete)         :: 142 of 226
            Pseudo Genes (internal stop)      :: 46 of 226
            Pseudo Genes (multiple problems)  :: 46 of 226
            Pseudo Genes (short protein)      :: 3 of 226
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095507.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="GN02050"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2050-4"
                     /geo_loc_name="USA"
                     /lat_lon="36 N 78.9 W"
                     /collection_date="2002"
                     /collected_by="Vance G. Fowler Jr."
                     /genotype="144"
     gene            <1..804
                     /locus_tag="MW398_RS26070"
                     /old_locus_tag="MW398_26075"
     CDS             <1..804
                     /locus_tag="MW398_RS26070"
                     /old_locus_tag="MW398_26075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007372656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="WP_268091495.1"
                     /translation="ETPDLVFQEPANVPLAGPANRQGRIAADNMLDRHSLYHGSQGTSI
                     CKVFSLSIGSVGANEKQLKAHGTRYEKVYVHAADHASYYPGATMISLKLLFSPDTGEIL
                     GAQASGKKGVDKRIDVLAVVQRAGLTVNDLEHLELTYAPPFNSARDVVNQAGMVAANVI
                     KGDTVICHVRDVLQREPGSYCLLDIRSPAELKQFGEYPDALSIPLDSLRENLDKLPEDK
                     EIIIGCQSGLRGHVAYRMLQSHGFRTYNLSGGFITWQAVTESMSQ"
                     /name="rhodanese-like domain-containing protein"
     gene            2117..3031
                     /locus_tag="MW398_RS26075"
                     /old_locus_tag="MW398_26080"
     CDS             2117..3031
                     /locus_tag="MW398_RS26075"
                     /old_locus_tag="MW398_26080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_023278182.1"
                     /translation="MSAALQYFEENLPLRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKICADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(3235..3633)
                     /locus_tag="MW398_RS26080"
                     /old_locus_tag="MW398_26085"
     CDS             complement(3235..3633)
                     /locus_tag="MW398_RS26080"
                     /old_locus_tag="MW398_26085"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001560793.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            3809..4735
                     /locus_tag="MW398_RS26085"
                     /old_locus_tag="MW398_26090"
     CDS             3809..4735
                     /locus_tag="MW398_RS26085"
                     /old_locus_tag="MW398_26090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_023144241.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPDRGGAKKDSGNLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYYRHLEQARMVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDGVSRENRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            4738..5271
                     /locus_tag="MW398_RS26090"
                     /old_locus_tag="MW398_26095"
     CDS             4738..5271
                     /locus_tag="MW398_RS26090"
                     /old_locus_tag="MW398_26095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_011666803.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAIEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(5325..5546)
                     /locus_tag="MW398_RS26095"
                     /old_locus_tag="MW398_26100"
     CDS             complement(5325..5546)
                     /locus_tag="MW398_RS26095"
                     /old_locus_tag="MW398_26100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024241855.1"
                     /translation="MTKLDENIPHIIDAAVDFMASSQAFREYLKKLPPDNTIPPDLTDE
                     HVPLYIRRLEYYRRLYRPKQTESYWKKR"
                     /name="hypothetical protein"
     gene            complement(6088..6435)
                     /locus_tag="MW398_RS26100"
                     /old_locus_tag="MW398_26105"
     CDS             complement(6088..6435)
                     /locus_tag="MW398_RS26100"
                     /old_locus_tag="MW398_26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329508.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_023144239.1"
                     /translation="MPMNEDTKRQTDMKNAALGAADVLRGLANSDRLLLMCQLSQGEAN
                     VTELEAAVGITQPTLSQQLGVMRRLKLVETRRAGKMVYYRIGDNRIMTLLNTLYDLYCP
                     QADPDKVKKQP"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            6596..8254
                     /locus_tag="MW398_RS26105"
                     /old_locus_tag="MW398_26110"
     CDS             6596..8254
                     /locus_tag="MW398_RS26105"
                     /old_locus_tag="MW398_26110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007372656.1"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_059342422.1"
                     /translation="MKIVIVGGVAGGASAAARARRLSEDVSIVVFERGSDVSFANCGLP
                     YHIGGKIPLRQSLILKTPEDFKSRFNIDVRIRHEVMSVDPVNKMVTVKNLTSGEVYTEE
                     WDRLLLSTGAAPVVPPLPGLQEAGVFTLRNLTDMDAIQAWIEQHNIAHTTLVGGGFIGL
                     EVMEALSERGISVTLLEMGEQVMAPVDPEMASALHQEIRSHGVDLRLRTALTEVLRTET
                     GFRVALSDGGFLQTDMVILAIGVKPESSLATGAGLAVGKRGGISVNACMQTSIPDIYAV
                     GDAVETPDLVFQEPANVPLAGPANRQGRIAADNMLDRHSLYHGSQGTSICKVFSLSIGS
                     VGANEKQLKAHGTRYEKVYVHAADHASYYPGATMISLKLLFSPDTGEILGAQASGKKGV
                     DKRIDVLAVVQRAGLTVNDLEHLELTYAPPFNSARDVVNQAGMVAANVIKGDTVICHVR
                     DVLQREPGSYCLLDIRSPAELKQFGEYPDALSIPLDSLRENLDKLPEDKEIIIGCQSGL
                     RGHVAYRMLQSHGFRTYNLSGGFITWQAVTESMSQ"
                     /name="FAD-dependent oxidoreductase"
     gene            9567..10481
                     /locus_tag="MW398_RS26110"
                     /old_locus_tag="MW398_26115"
     CDS             9567..10481
                     /locus_tag="MW398_RS26110"
                     /old_locus_tag="MW398_26115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_023278182.1"
                     /translation="MSAALQYFEENLPLRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKICADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(10685..>10934)
                     /locus_tag="MW398_RS26115"
                     /old_locus_tag="MW398_26120"
                     /pseudo=""
     CDS             complement(10685..>10934)
                     /locus_tag="MW398_RS26115"
                     /old_locus_tag="MW398_26120"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /pseudo=""
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     Typing          2118..3031
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.017"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9568..10481
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.017"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3810..4732
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaaacacctg accttgtctt tcaggaaccc gctaatgttc ctctggcagg gccagccaac
       61 cgccagggac gcattgctgc tgataatatg ctcgatcgtc acagccttta ccatggcagt
      121 caggggacat ccatttgtaa ggtattttcg ctgagtattg gcagtgtcgg ggcgaatgaa
      181 aagcagctga aagcccatgg tacccgatac gagaaggttt acgtccatgc cgctgatcat
      241 gccagttact acccgggggc aacaatgatc agtctgaaac tgctgttcag ccccgatacc
      301 ggtgaaattc tcggggccca ggcgtcaggg aaaaaagggg ttgataaacg catcgatgtc
      361 ctggctgtcg tacagcgcgc ggggcttacc gttaacgatc tcgagcatct ggagctgacc
      421 tatgctccgc ctttcaacag tgcaagggac gtcgttaacc aggctggaat ggtggcggca
      481 aatgttatca agggcgatac ggtcatctgt catgtccgcg atgtgttaca acgggaaccg
      541 ggcagttatt gtttacttga tattcgttct cctgcagaac tgaaacaatt tggtgaatat
      601 cctgacgcgc tatccatccc tcttgattcc ctgcgggaga atcttgataa gttgcctgaa
      661 gataaagaga ttattatcgg gtgtcagagt ggtctgcggg ggcatgtggc ataccgtatg
      721 cttcagtctc acggcttccg gacatataat ctgagtggtg gctttataac ttggcaggcg
      781 gttacggaat caatgagtca ataagagatg aggtgccata cacatggcac ctcatcttcc
      841 agattcattg aaaacgtatt atatggaata gaaatctaca aaaataatta acaccctgga
      901 tggtacccta ttgcgataat gtggactgtt caaaaaacag gcttaccgta caataattct
      961 ctatatccaa actgacccca atgttcctga atgtaaatct ccgtacaaag atatcgttga
     1021 gttttatata attcattgtt tttattgtgt tttgtttttt tggttgtgct ggtttgtagt
     1081 tataaaaaac ccctgggaat ccaggggtag gtaaggaaaa caaatgatac tcagttagtg
     1141 ttgcacttta aacgggttga agtataactc gtattaaatg agatgttgaa aataagtgaa
     1201 aaccattgct ggttgcaata gggcagtatg cgtaaaaaat aaatttgtga tgcttttcaa
     1261 aaaaacaatt aatacatgaa tattacagct catatattac agtttataaa aaaactgtgt
     1321 tttatctatt acatagagaa aaattcttat cctgagctgt gaacttggtt gtttcgtttg
     1381 atatatatct ttgtaaatgt ggtttattat gcgggcattt tttattgagt attgaatcag
     1441 aataagtgat gcagctaaat ttatttgcgt aactatttca ttgtcaatat tctgactcaa
     1501 tctgacacct atcgtttctc tttgattccc cacttttgtg ttttttttat ccggaacggg
     1561 atagtgccag ttgtttttta tcagcctcag ggtattttgg ggcggttgcg aacattgacg
     1621 cattccacaa ctaaaaaaaa tttctcacag actctgattc ttgccccgac ccttggtcgg
     1681 tttttttttg tcctggcgaa acgtgaagct ctgaaacaga aaatctccgg gcagaacctt
     1741 ccatccgggg ggagaaaact tttcgttttg agccgttcgg tgttcagaac gcacgaaacc
     1801 gatcgcacat cgctttcgtg catagttatg caacgccctg aaaacgattc tgacgcgttt
     1861 ttctggtttt gcctggtgtt ttcttgtctt tttgcgtttt ttgcgccaga aagcgactga
     1921 gggcgtttta aggggggcgt acaacgggag ttatggtaaa tggatcggtt tttcgggaag
     1981 gatcgacagg atttgccgtt gggtgtagtg taagcgactg aaaaacaaac gcccctgaaa
     2041 ttcgtgtctc tccgccaaga ttgtcacgag aatacagggg cgaggtcggt cgataacctg
     2101 gaaggaatcg taacacatga gcgccgcgct tcaatacttc gaagaaaatt tacccctccg
     2161 cccctatcac acggatgatc tcgctttcgg acttcgtatc tccggtaaag ggcgtgcgct
     2221 tcttgcacgg tatatccagc agaaccagcc tcatgcacag ttctggctgg tttttgacgt
     2281 tgaccgcgag ggggctgcga ttgactggag cgaccggaac gcccccgcgc cgaacatcac
     2341 cgttaaaaat cctgtgaacg gacacgctca cctgctctac gcactcaaca tcgccgtgag
     2401 aaccgcgcct gatgcctcgg ttaaggcgct gaaatatgcc gcagcggttg aacgttcgct
     2461 gtgtgagaaa atttgtgctg atgtgaacta cagcggcctg atttgcaaaa atccgttcca
     2521 cctggaatgg caggtgatgg agtggcgtga ggaagcctat acactcgatg aactggctga
     2581 ttatctcgat ttgagcgcct cagcgcgtcg tagcatcgat aaacattacg ggatggggcg
     2641 aaactgccac ctgttcgaaa tgacgcgcaa atgggcttac agggcgattc gtcagggatg
     2701 gccagcattc tcacagtggc ttgatgcggt gattcagcgt gtcgaaatgt acaatgcatc
     2761 gcttcccgtt ccgctttcac cgcctgaatg tcgggctatt ggcaagagta ttgcgaaata
     2821 cacgcacagg aacttcacgc cggaaacttt cgcacagtat gtggctgata cgcacacacc
     2881 agaaattcag gctgcacgcg gtcgcaaggg cggttctaag tctaagcgca gcacagtagc
     2941 tacatctgca cgcacgctga aaccgtggga ggcattaggg atcagtcgcg cctggtatta
     3001 ccaactgaaa aaacgaggtc tcgtagagta gaccaaataa gcctatatca gataacagcg
     3061 cctttctggc gtctttttga gcagcaggtt ttttgccgtt tttccccgtt cagccctgca
     3121 atggcgcggc tttccgcgtg attgtggtgg tagtgctcgc cgcagtctca tgaccgagcg
     3181 tagcgagcga atgagcgagg aagcaccagg gaatcggaac acggatggag gcacttacgc
     3241 gtctgggttt ggggatgtct ccagatcgta cttaagttcc ttaagtgctt gtaatatcct
     3301 gtctttttta tagcatagct cgcctgtttt tgtgtctacc agtcctgctt cgatcagtag
     3361 cccagccgcg agaaagagcc ggtgattgcg ttcccgtcgt tgtttttttc cctcgattcg
     3421 tttttggacc gcctgttttc gttcgctctc ctgtgcctgt aagtcgtttt tttccgctgt
     3481 tgccagtacg tggaggagtt tgcgttcctg ttctgtgagt cctgcttcgt tgtcgagcag
     3541 gtcaataatc tgtctctgtc gtgcggtctg ttctccgagc ttcctgatga atccgatccg
     3601 tttcttcagc cattcgatgt tgctgatgcg catgttcgtg gtgctccctg gtgttttttg
     3661 ctgtgtgttg tggtcgtgca gtatatcgct tgcggtcggt gggtgcaggg ggtatcttcc
     3721 gtgctaattc gtaaaattag aaggaagcgc acttatatgt ttctctcctt cgtcgagaaa
     3781 cagtgcgctc ctgcagaggg ctgaaaccgt ggcaacctat cacctcagcg ttaaatttgg
     3841 tggcaagggg caggctgcaa accacgccga ttacatcgag cgcaaagaga aataccgcga
     3901 ccgtcaggat ttggaatatt ccgcacacgg aaacatgccg gaatgggcca gggataatcc
     3961 ttcccatttc tggcaggcgg ctgaccagtt tgaacgtgcg aacggttcga cctataggga
     4021 acttgaaatt gcattacccc gtgaactgac accggaacag cgccttgaac tggtgcagga
     4081 ttttgtacgt caggaagctg gcgaacggca cgcctggagt tttgccattc acaatccgaa
     4141 agcgagcatt gacggcggcg aacagcctca tgcgcatatc atgatgagtc agcgtgtgaa
     4201 tgatggcatt gaacgtacac cggaacagta tttccggcgc tataacgcca ggtatcccga
     4261 ccggggaggt gcgaaaaagg attccggaaa cctgaccccc actcagcaaa aagagcagtt
     4321 gcgggaattg cgaaaacgct gggaggtgaa gcacaacgaa catatgagaa agcatggctt
     4381 tgaaaggggc ttcattgact gccggacact gaaagaacag ggcattgaac gacgtcccga
     4441 agtgcatctg ggttttgaag ctgccggacg acttgatgat gcccagcgtc atgacatcat
     4501 gcaaaaacgg tctgaaccgg actattaccg ccatcttgaa caggcgcgaa tggtcgggca
     4561 tgacatcagg gaatggctgg atacaccggt taagcaacat gaactggtca gaaggctgca
     4621 acatgatgtt gcgcgggagc atggctttga catgagagac ggagtgagca gagagaaccg
     4681 aactgaacct gaacgggtta aatcaccgtc acgtggattt tctatgggat tctgaacatg
     4741 gccagcacag aagaaaaatt actcacgctc ctgttatcgg tggagcatct gcaacatacc
     4801 gcaatagagc aacagaaagc tgttgctgat tctctggctg tggcaaggga aactcttcgc
     4861 ggatatcggg atatttctga cggtgtggca aaacaggtca gggatgaagt cagaaacgag
     4921 gtgcagaagc tggacgtggc aggactgata ggtgagcgca tcagccagga ttttcagcgg
     4981 ctggaagagt cagtcgcaaa catgaaggca aacgcggatg ccctgaacag ggatattgcg
     5041 acaattcaca gcaatatcgt gcaggaatac aacaccctta aagggtatag ctggaaatgg
     5101 ctgggtgggg tgtcagtggc ttttctggtt atgttctgtg tgctcggatg gggaatgaaa
     5161 acaatactcg acaggaacta caacgccaca gcggcggtgt atcagaaagt ggtgacactg
     5221 gaagataaac tggtggagaa acctgtcacg gggaagagcc ggaaaaactg acgcgcttcg
     5281 cttgtccaac gcctcacgga gcgcgaaagg atttcgcgaa ccgttcaccg ttttttccaa
     5341 tatgattctg tctgctttgg tcgataaagc cgtcgatagt attccagcct tcgtatatat
     5401 aacggtacgt gctcatcagt aagatctggc ggtattgtgt tatcaggggg aagttttttc
     5461 agatactcac gaaatgcctg gcttgatgcc ataaaatcta cagctgcatc gataatatgt
     5521 gggatatttt catctaattt tgtcatgatg attgatctga tattatcacg gttcgtgaat
     5581 tatagtatgt cactattatt tatgcgttag tgaaccagaa aaaatccccc gttggtatag
     5641 ggggatatgt aaggagaaat aacttcgcag gtgttcttgt ttgtattata acgtgaaaag
     5701 aatgcacgca acttaattaa tctgccgtac ttcctttcag ttttcaagat aaggtttata
     5761 ataaaacatg gaatggaagc aagctttaag gtttcagctc taaaattttt aatatctatt
     5821 aactagctag ttgttgtcct tgtgagcatg ttgtatataa aagctaggat aatatcctga
     5881 ccggagtatt catattcctt atcccctctg tgttctcaga ggtttttttc tgtatttaat
     5941 gatatgagct ggaatgcgat tccggccccc ccgtttacat aattctccct gacatacggc
     6001 accggaatcg cattgatttt gcattgttaa tgtctaactg tgctggtgaa tgttgggggc
     6061 agtttggata tcgaaaatta ttgtacgtta aggttgtttt ttgaccttgt cggggtccgc
     6121 ctgcgggcag tagagatcat ataaggtatt caataacgtc atgatgcggt tatccccgat
     6181 acggtaatat accattttac ctgcccgtcg ggtctcgacc agcttcaggc gacgcatgac
     6241 acccaactgc tgagacaggg tcggctgagt gataccgacc gccgcttcca gctcggtcac
     6301 attcgcttcc ccctggctta actgacacat caacaatagc cggtcgctgt tggccagtcc
     6361 ccgtaggaca tctgctgccc ccagggctgc atttttcata tcagtctgac gttttgtgtc
     6421 ttcattcatg ggcattgctg gtttcttata ttatgtattt ttatataata ttgttcagta
     6481 taatgatagt ggcgtaattt tgccaccgtg atcttcaggt ctgcagcgtc cagctggacc
     6541 gggtaactaa ttatatatgg cagatatttt gccccgagtg aacagagagg atgtaatgaa
     6601 aatagtgatt gttggtggtg ttgccggggg agcatccgct gcagccaggg ctcgtcggtt
     6661 atcagaggat gtcagtattg ttgtgtttga gcggggaagc gatgtctctt tcgccaactg
     6721 tggattgcct tatcatatag gcggaaaaat tccgttacgg cagtcgctga ttctgaaaac
     6781 cccggaggat tttaaatccc gttttaacat agacgtccgt atccgtcatg aagtgatgtc
     6841 agtagacccg gttaataaga tggtgacggt taaaaatctg acctccgggg aggtctatac
     6901 cgaagaatgg gatcgtctgc tcctcagtac cggtgccgct ccggttgttc cccccttgcc
     6961 cggactacag gaagcgggtg tctttacact gcgaaatctt actgacatgg atgctatcca
     7021 ggcgtggatt gagcaacata atatcgctca caccacgctt gtcggggggg gatttatcgg
     7081 gctcgaagtg atggaggcac tgagcgaaag ggggatcagt gtgaccctgc tggagatggg
     7141 ggaacaggtt atggctccgg ttgatcctga aatggcatcg gccctgcatc aggaaatcag
     7201 gagtcatggc gtggatctgc gtctcagaac ggcgctcacc gaggtactgc ggacagagac
     7261 gggattccgc gttgcactgt ctgacggtgg gtttctgcag accgacatgg ttattctcgc
     7321 catcggggta aaacctgaga gcagccttgc cacaggagcc gggctcgccg tgggtaaacg
     7381 gggaggcata agtgtcaatg cctgtatgca gacaagtatc cctgacatct atgccgtggg
     7441 ggatgcagtt gaaacacctg accttgtctt tcaggaaccc gctaatgttc ctctggcagg
     7501 gccagccaac cgccagggac gcattgctgc tgataatatg ctcgatcgtc acagccttta
     7561 ccatggcagt caggggacat ccatttgtaa ggtattttcg ctgagtattg gcagtgtcgg
     7621 ggcgaatgaa aagcagctga aagcccatgg tacccgatac gagaaggttt acgtccatgc
     7681 cgctgatcat gccagttact acccgggggc aacaatgatc agtctgaaac tgctgttcag
     7741 ccccgatacc ggtgaaattc tcggggccca ggcgtcaggg aaaaaagggg ttgataaacg
     7801 catcgatgtc ctggctgtcg tacagcgcgc ggggcttacc gttaacgatc tcgagcatct
     7861 ggagctgacc tatgctccgc ctttcaacag tgcaagggac gtcgttaacc aggctggaat
     7921 ggtggcggca aatgttatca agggcgatac ggtcatctgt catgtccgcg atgtgttaca
     7981 acgggaaccg ggcagttatt gtttacttga tattcgttct cctgcagaac tgaaacaatt
     8041 tggtgaatat cctgacgcgc tatccatccc tcttgattcc ctgcgggaga atcttgataa
     8101 gttgcctgaa gataaagaga ttattatcgg gtgtcagagt ggtctgcggg ggcatgtggc
     8161 ataccgtatg cttcagtctc acggcttccg gacatataat ctgagtggtg gctttataac
     8221 ttggcaggcg gttacggaat caatgagtca ataagagatg aggtgccata cacatggcac
     8281 ctcatcttcc agattcattg aaaacgtatt atatggaata gaaatctaca aaaataatta
     8341 acaccctgga tggtacccta ttgcgataat gtggactgtt caaaaaacag gcttaccgta
     8401 caataattct ctatatccaa actgacccca atgttcctga atgtaaatct ccgtacaaag
     8461 atatcgttga gttttatata attcattgtt tttattgtgt tttgtttttt tggttgtgct
     8521 ggtttgtagt tataaaaaac ccctgggaat ccaggggtag gtaaggaaaa caaatgatac
     8581 tcagttagtg ttgcacttta aacgggttga agtataactc gtattaaatg agatgttgaa
     8641 aataagtgaa aaccattgct ggttgcaata gggcagtatg cgtaaaaaat aaatttgtga
     8701 tgcttttcaa aaaaacaatt aatacatgaa tattacagct catatattac agtttataaa
     8761 aaaactgtgt tttatctatt acatagagaa aaattcttat cctgagctgt gaacttggtt
     8821 gtttcgtttg atatatatct ttgtaaatgt ggtttattat gcgggcattt tttattgagt
     8881 attgaatcag aataagtgat gcagctaaat ttatttgcgt aactatttca ttgtcaatat
     8941 tctgactcaa tctgacacct atcgtttctc tttgattccc cacttttgtg ttttttttat
     9001 ccggaacggg atagtgccag ttgtttttta tcagcctcag ggtattttgg ggcggttgcg
     9061 aacattgacg cattccacaa ctaaaaaaaa tttctcacag actctgattc ttgccccgac
     9121 ccttggtcgg tttttttttg tcctggcgaa acgtgaagct ctgaaacaga aaatctccgg
     9181 gcagaacctt ccatccgggg ggagaaaact tttcgttttg agccgttcgg tgttcagaac
     9241 gcacgaaacc gatcgcacat cgctttcgtg catagttatg caacgccctg aaaacgattc
     9301 tgacgcgttt ttctggtttt gcctggtgtt ttcttgtctt tttgcgtttt ttgcgccaga
     9361 aagcgactga gggcgtttta aggggggcgt acaacgggag ttatggtaaa tggatcggtt
     9421 tttcgggaag gatcgacagg atttgccgtt gggtgtagtg taagcgactg aaaaacaaac
     9481 gcccctgaaa ttcgtgtctc tccgccaaga ttgtcacgag aatacagggg cgaggtcggt
     9541 cgataacctg gaaggaatcg taacacatga gcgccgcgct tcaatacttc gaagaaaatt
     9601 tacccctccg cccctatcac acggatgatc tcgctttcgg acttcgtatc tccggtaaag
     9661 ggcgtgcgct tcttgcacgg tatatccagc agaaccagcc tcatgcacag ttctggctgg
     9721 tttttgacgt tgaccgcgag ggggctgcga ttgactggag cgaccggaac gcccccgcgc
     9781 cgaacatcac cgttaaaaat cctgtgaacg gacacgctca cctgctctac gcactcaaca
     9841 tcgccgtgag aaccgcgcct gatgcctcgg ttaaggcgct gaaatatgcc gcagcggttg
     9901 aacgttcgct gtgtgagaaa atttgtgctg atgtgaacta cagcggcctg atttgcaaaa
     9961 atccgttcca cctggaatgg caggtgatgg agtggcgtga ggaagcctat acactcgatg
    10021 aactggctga ttatctcgat ttgagcgcct cagcgcgtcg tagcatcgat aaacattacg
    10081 ggatggggcg aaactgccac ctgttcgaaa tgacgcgcaa atgggcttac agggcgattc
    10141 gtcagggatg gccagcattc tcacagtggc ttgatgcggt gattcagcgt gtcgaaatgt
    10201 acaatgcatc gcttcccgtt ccgctttcac cgcctgaatg tcgggctatt ggcaagagta
    10261 ttgcgaaata cacgcacagg aacttcacgc cggaaacttt cgcacagtat gtggctgata
    10321 cgcacacacc agaaattcag gctgcacgcg gtcgcaaggg cggttctaag tctaagcgca
    10381 gcacagtagc tacatctgca cgcacgctga aaccgtggga ggcattaggg atcagtcgcg
    10441 cctggtatta ccaactgaaa aaacgaggtc tcgtagagta gaccaaataa gcctatatca
    10501 gataacagcg cctttctggc gtctttttga gcagcaggtt ttttgccgtt tttccccgtt
    10561 cagccctgca atggcgcggc tttccgcgtg attgtggtgg tagtgctcgc cgcagtctca
    10621 tgaccgagcg tagcgagcga atgagcgagg aagcaccagg gaatcggaac acggatggag
    10681 gcacttacgc gtctgggttt ggggatgtct ccagatcgta cttaagttcc ttaagtgctt
    10741 gtaatatcct gtctttttta tagcatagct cgcctgtttt tgtgtctacc agtcctgctt
    10801 cgatcagtag cccagccgcg agaaagagcc ggtgattgcg ttcccgtcgt tgtttttttc
    10861 cctcgattcg tttttggacc gcctgttttc gttcgctctc ctgtgcctgt aagtcgtttt
    10921 tttccgctgt tgcc
//
