LOCUS       NZ_CP095503            11795 bp    DNA     linear   CON 20-JAN-2024
DEFINITION  Escherichia coli strain GN02050 plasmid p2050-2.
ACCESSION   NZ_CP095503
VERSION     NZ_CP095503.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27361453
            Assembly: GCF_026651085.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11795)
  AUTHORS   Bethke,J.H., Ma,H.R., Tsoi,R., Cheng,L., Xiao,M. and You,L.
  TITLE     Vertical and horizontal gene transfer tradeoffs direct plasmid
            fitness
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11795)
  AUTHORS   Bethke,J.H., Ma,H.R., Tsoi,R., Cheng,L., Xiao,M. and You,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2022) Molecular Genetics and Microbiology, Duke
            University, CIEMAS Building 101 Science Drive, Durham, NC 27705, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 6.0.0.47841
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 508x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/20/2024 04:55:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,269
            CDSs (total)                      :: 5,143
            Genes (coding)                    :: 4,917
            CDSs (with protein)               :: 4,917
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 89 of 226
            Pseudo Genes (incomplete)         :: 142 of 226
            Pseudo Genes (internal stop)      :: 46 of 226
            Pseudo Genes (multiple problems)  :: 46 of 226
            Pseudo Genes (short protein)      :: 3 of 226
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095503.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="GN02050"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2050-2"
                     /geo_loc_name="USA"
                     /lat_lon="36 N 78.9 W"
                     /collection_date="2002"
                     /collected_by="Vance G. Fowler Jr."
                     /genotype="144"
     gene            66..350
                     /locus_tag="MW398_RS00080"
                     /old_locus_tag="MW398_00080"
     CDS             66..350
                     /locus_tag="MW398_RS00080"
                     /old_locus_tag="MW398_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Z C-terminal domain-related protein"
                     /protein_id="WP_000625497.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIAF
                     IWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
                     /name="colicin Z C-terminal domain-related protein"
     gene            complement(414..803)
                     /locus_tag="MW398_RS00085"
                     /old_locus_tag="MW398_00085"
     CDS             complement(414..803)
                     /locus_tag="MW398_RS00085"
                     /old_locus_tag="MW398_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000477210.1"
                     /translation="MFKSSVKSITSSTILTLLIGCSSTETNKNIVYKVGGDSIILNKYC
                     ILKMYPSQSEKNKGVIIEVNTDSLCKKKLNEITSKNIGKPLISYFDGKVISQPAIIHSA
                     LNPTHYYQPVNDETTVMEIIKAYSK"
                     /name="hypothetical protein"
     gene            complement(1210..1323)
                     /locus_tag="MW398_RS26195"
     CDS             complement(1210..1323)
                     /locus_tag="MW398_RS26195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001427889.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGN"
                     /name="hypothetical protein"
     gene            complement(1991..2182)
                     /locus_tag="MW398_RS00090"
                     /old_locus_tag="MW398_00090"
     CDS             complement(1991..2182)
                     /locus_tag="MW398_RS00090"
                     /old_locus_tag="MW398_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165988.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDSENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2247..2570
                     /locus_tag="MW398_RS00095"
                     /old_locus_tag="MW398_00095"
     CDS             2247..2570
                     /locus_tag="MW398_RS00095"
                     /old_locus_tag="MW398_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000956005.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2779..>3210
                     /locus_tag="MW398_RS00100"
                     /old_locus_tag="MW398_00100"
                     /pseudo=""
     CDS             2779..>3210
                     /locus_tag="MW398_RS00100"
                     /old_locus_tag="MW398_00100"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3248..3766
                     /locus_tag="MW398_RS00105"
                     /old_locus_tag="MW398_00105"
     CDS             3248..3766
                     /locus_tag="MW398_RS00105"
                     /old_locus_tag="MW398_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023144181.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETILNDEFRKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSC
                     GTLWYLGGMIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPHQ
                     GKDWVQLME"
                     /name="MbeB family mobilization protein"
     gene            3773..4006
                     /locus_tag="MW398_RS00110"
                     /old_locus_tag="MW398_00110"
     CDS             3773..4006
                     /locus_tag="MW398_RS00110"
                     /old_locus_tag="MW398_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001555310.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4553..4849)
                     /locus_tag="MW398_RS00115"
                     /old_locus_tag="MW398_00115"
     CDS             complement(4553..4849)
                     /locus_tag="MW398_RS00115"
                     /old_locus_tag="MW398_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047647.1"
                     /translation="MSKTKSGRHRLSKTDKRLLAALIAAGYEERTARDLIQKHVYTLTQ
                     ADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKFLNGKKPEEGLNAWKGDLP"
                     /name="hypothetical protein"
     gene            5061..5258
                     /locus_tag="MW398_RS00120"
                     /old_locus_tag="MW398_00120"
     CDS             5061..5258
                     /locus_tag="MW398_RS00120"
                     /old_locus_tag="MW398_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072251766.1"
                     /translation="MKEEKVLLHRFLFVVRNKNGCELSCSADLMGTRDDVYKYFSDSVS
                     ELDVELIDVSCESEWEEHSH"
                     /name="hypothetical protein"
     gene            5276..5560
                     /locus_tag="MW398_RS00125"
                     /old_locus_tag="MW398_00125"
     CDS             5276..5560
                     /locus_tag="MW398_RS00125"
                     /old_locus_tag="MW398_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Z C-terminal domain-related protein"
                     /protein_id="WP_000625497.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIAF
                     IWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
                     /name="colicin Z C-terminal domain-related protein"
     gene            complement(5624..6013)
                     /locus_tag="MW398_RS00130"
                     /old_locus_tag="MW398_00130"
     CDS             complement(5624..6013)
                     /locus_tag="MW398_RS00130"
                     /old_locus_tag="MW398_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000477210.1"
                     /translation="MFKSSVKSITSSTILTLLIGCSSTETNKNIVYKVGGDSIILNKYC
                     ILKMYPSQSEKNKGVIIEVNTDSLCKKKLNEITSKNIGKPLISYFDGKVISQPAIIHSA
                     LNPTHYYQPVNDETTVMEIIKAYSK"
                     /name="hypothetical protein"
     gene            complement(6420..6533)
                     /locus_tag="MW398_RS26200"
     CDS             complement(6420..6533)
                     /locus_tag="MW398_RS26200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001427889.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGN"
                     /name="hypothetical protein"
     gene            complement(7201..7392)
                     /locus_tag="MW398_RS00135"
                     /old_locus_tag="MW398_00135"
     CDS             complement(7201..7392)
                     /locus_tag="MW398_RS00135"
                     /old_locus_tag="MW398_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165988.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDSENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            7457..7780
                     /locus_tag="MW398_RS00140"
                     /old_locus_tag="MW398_00140"
     CDS             7457..7780
                     /locus_tag="MW398_RS00140"
                     /old_locus_tag="MW398_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000956005.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7989..>8420
                     /locus_tag="MW398_RS00145"
                     /old_locus_tag="MW398_00145"
                     /pseudo=""
     CDS             7989..>8420
                     /locus_tag="MW398_RS00145"
                     /old_locus_tag="MW398_00145"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            8458..8976
                     /locus_tag="MW398_RS00150"
                     /old_locus_tag="MW398_00150"
     CDS             8458..8976
                     /locus_tag="MW398_RS00150"
                     /old_locus_tag="MW398_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023144181.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETILNDEFRKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSC
                     GTLWYLGGMIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPHQ
                     GKDWVQLME"
                     /name="MbeB family mobilization protein"
     gene            8983..9216
                     /locus_tag="MW398_RS00155"
                     /old_locus_tag="MW398_00155"
     CDS             8983..9216
                     /locus_tag="MW398_RS00155"
                     /old_locus_tag="MW398_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001555310.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(9763..10059)
                     /locus_tag="MW398_RS00160"
                     /old_locus_tag="MW398_00160"
     CDS             complement(9763..10059)
                     /locus_tag="MW398_RS00160"
                     /old_locus_tag="MW398_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047647.1"
                     /translation="MSKTKSGRHRLSKTDKRLLAALIAAGYEERTARDLIQKHVYTLTQ
                     ADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKFLNGKKPEEGLNAWKGDLP"
                     /name="hypothetical protein"
     gene            10481..10765
                     /locus_tag="MW398_RS00165"
                     /old_locus_tag="MW398_00165"
     CDS             10481..10765
                     /locus_tag="MW398_RS00165"
                     /old_locus_tag="MW398_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Z C-terminal domain-related protein"
                     /protein_id="WP_000625497.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIAF
                     IWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
                     /name="colicin Z C-terminal domain-related protein"
     gene            complement(10829..11218)
                     /locus_tag="MW398_RS00170"
                     /old_locus_tag="MW398_00170"
     CDS             complement(10829..11218)
                     /locus_tag="MW398_RS00170"
                     /old_locus_tag="MW398_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000477210.1"
                     /translation="MFKSSVKSITSSTILTLLIGCSSTETNKNIVYKVGGDSIILNKYC
                     ILKMYPSQSEKNKGVIIEVNTDSLCKKKLNEITSKNIGKPLISYFDGKVISQPAIIHSA
                     LNPTHYYQPVNDETTVMEIIKAYSK"
                     /name="hypothetical protein"
     gene            complement(11624..11737)
                     /locus_tag="MW398_RS26205"
     CDS             complement(11624..11737)
                     /locus_tag="MW398_RS26205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001427889.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGN"
                     /name="hypothetical protein"
     Typing          1819..1906
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          7029..7116
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(1252..1808)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6462..7018)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11629..11795)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.619"
                     /coverage="100.0"
                     /evalue="8.7000000000000015e-75"
     Typing          2561..4110
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.555"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7771..9320
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.555"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttatagatg tttcttgcga gagtgaatgg gaagaacatt cgcattgaac taatcaggag
       61 attatatgat ttctcaaaat gtttgggctc ctcctttctt ttttggtcct tgggttgaac
      121 ttgaaaatca tactggtgat atcgcgtaca ctgtctattt tgatacattg tctaatgtac
      181 ccggtccatt tattgctttt atctggtatg cccctaatgg gccaagaaca gttaacgtta
      241 ttggtccagc atcttatcag atctcttgca agggcgctgg tgttgataaa attaaattta
      301 aaagtgtcag tgggacaggg cagattattc atgtatacac taccttataa taagagctga
      361 ataaatataa tcctgcctaa gctatacctt aagcaggatt atatctatta atattatttt
      421 gaatatgctt ttatgatttc catcactgtt gtttcatcgt tcacaggttg ataataatgg
      481 gttggattta gtgcagaatg aatgattgca ggttgggata ttacttttcc atcaaaatat
      541 gaaatcaggg gtttccctat atttttacta gttatttcgt ttagtttttt cttacataaa
      601 ctatctgtgt ttacttctat tatgacgcct ttgttttttt ctgattggga agggtacatt
      661 ttaagtatgc aatatttatt cagaatgatg ctgtcaccac caactttgta aacaatgttt
      721 ttgtttgttt cagtacttga acatcctata agtaaggtca gtatagtact cgatgttatt
      781 gactttactg atgatttgaa catattaact ccattttatt ttgttaaaat tccaatctat
      841 aaatacatat acacgtaaaa ataaacaatg cgaacgtttt cacttatatt atccccatga
      901 tgtgattgaa tggtggttcc atataagaga gggcaattat actatctttc tctcctgaac
      961 tgatgggatt gcagtttaga gaaaaaaaga tcaaaggatc ttcttgagat cctttttttc
     1021 tgcgcgtaat ctgctgcctg taaacgaaaa aaccaccctg gcaggtggtt tttcgaaggt
     1081 tagctaatcc tggcagatta cctaaccggg tagctggctt aagtggagca cagataccaa
     1141 aagatgtcct ttcagtgtag ccgctataag gcttcaactt caagagttct gcgtatctaa
     1201 atccactttt tagttaccaa tggctgctgc cagtggcgtt aagtcgtgtc ttaccgggtt
     1261 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1321 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1381 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taaacggtaa
     1441 ggtcggaaca ggagagcgca cgagggagct tccgggggga aacgcctggt atctttaagt
     1501 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1561 cggagcctat ggaaaaacgc cagcggcgcg gccttcttcc tgttcctggt cttttgctca
     1621 catgtttttt ccggtgttat cccctgattc tgtggataac agcatttcgt ttttgagtga
     1681 gtagataccg ctcgccgcag ccgaacgacc gagcgtagcg agtcagtgag cgaggaagcg
     1741 gaaaagcgcc tggacgtgca ttttctcctt acgcatctgt gcggcatttc acacccggta
     1801 tggcgcacgt ttcatacaat ccgcactgat gccgcatggt taagccagta tacactccgc
     1861 tatcgctacg tgactggttc agggctgcgc cccgaaaccc gctaaaacac cctgacgcgc
     1921 cctggcgggc ttgtctgctc ccggcatccg ctcacaggca agctgtgacc gtctccggga
     1981 gctgcatatg tcagaggttt tcactgtcat ccccgaagcg cgcgaggcag ctgcggtaaa
     2041 gctcatcggc gtggtcgtga agcgattcac agatatctgc ctgttcatcc gcgtccagtt
     2101 cgttgagctt ctccagcagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg
     2161 cggttttttc ctgtttagtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg
     2221 taatgatacc gatgaaacgc gagaggatgc tcacgatacg ggttactgat gatgaacatg
     2281 cccggttact ggaacgctgt gagggtaaac aactggcggt atggatgcgg cgggtctgcc
     2341 tgggtgagcc ggttgcccgt tccgggaggc tgccgacact ggcaccgccg ttactgcgtc
     2401 agctggcagc catcgggaat aacctgaacc agacagcccg taaggtgaac agcggacagt
     2461 ggtcttccgg cgaccgggtt caggtggtgg ccgcactgat ggccatcggg gatgagctgc
     2521 gccggttgcg tctggccgtc agggaacagg ggacgcggga tgatagttaa gtttcatgcc
     2581 cggggaaaag gtggtggcag tggtccggtt gattacctgc tgggaaggga gcgtaaccgg
     2641 gaaggggcaa cggtgcttca gggtaatccg gaagaagtac gggaactcat cgatgccacg
     2701 ccatttgcga agaaatacac gtccggtgtg ctgtcgttcg cggagaagga gctgccaccg
     2761 ggaggacgtg aaaaggtgat ggcgagcttt gagcgtgtac tgatgcccgg tctcgaaaaa
     2821 aatcagtaca gcatcctgtg ggtggagcac caggacaagg gacggcttga gctgaatttt
     2881 gtcattccga acatggagct acagaccgga aaacgcctcc agccgtacta cgaccgggca
     2941 gacaggccga gaattgatgc ctggcagacg ctggtgaatc accattacgg gctgcatgac
     3001 cccaacgccc cggagaaccg ccggatactg actctctctg ataacctgcc ggaaacgaaa
     3061 caggcgcttg cagagagcgt cacacgcggg atagacgctc tttatcatgt cggggagata
     3121 aaaggccgtc aggatgtgat tcaggcgctc acagaggcag ggcttgaagt ggtcagggtg
     3181 acgcgaagca gtatcagcat tgcagacccg aacggtggga agaatatcag gctgaaagga
     3241 gcattttatg agcaatcttt tacagacggg cgcggagttc gagaaaaagc tgaaagagag
     3301 agccgaatct acagagacaa tactgaacga cgagttcagg aagctcgaaa aatctgtaag
     3361 caaggctgtg acatcaaacg agacgaaaat caaagacgct atagccctgt tcacagcttc
     3421 gacagaggaa tcactgaaaa agcaccggga aggggtgaaa gaggcgatga tgcagcacag
     3481 gaaggacgtg ttaaagctgg cagggaatac gggcatgatg ttactgggga tggtcatttt
     3541 cctgtttacc gtgagttgcg ggacgctctg gtatcttggc gggatgatac aggcgaacct
     3601 ggaagatatc aggaagcagg aagagacatt gcagaaactg aacgcgaaga catggggcgt
     3661 ggagttcgta caggacggga acaggaaatt tctggtcctt ccgtacggga aatcagcaga
     3721 ggtgattccc catcagggga aggactgggt acaactgatg gagtgacaca gagtgacgga
     3781 gctggaaaga catttgctga acgcattaga gcaactgcaa caggactata tgcagcggct
     3841 gaacgaatgg gagagcgcct tcgtggaatt gcagaaaatg ttttcgctta cgcaacggga
     3901 caacgcgatg ctgaacgagc gggtcatgaa gttgagtcag caggtgcagc acttgagcga
     3961 gcggacagaa cgcttgagcc agttatacag cgagaacagg agataaggga cgaacggctg
     4021 atacaggaac gcgaacatga gttatccctg gagcgtgagc gccagctgga aatacaggaa
     4081 cggacactgg atggcccttc gctgggatgg taatcagaga caggctaaaa tgttgtcagc
     4141 gcgtttccgg tgcatttttt gggataatgg ttggttcggg gcaccgggaa cgcgcttcag
     4201 tcataaacct tgctgaaaac acaggaacgc gttaaaacgc atacgggcgc gattctggtg
     4261 tgtctttcat agtcgtgttt ttgcctctat tgccagatcg cgctcattcc cctgaacttc
     4321 tgttttcctg ctcaaatccc cgtcttcttc gcttccgaaa gtatgaaaaa aatccgtttc
     4381 agcggtctct gactggcaat tcctgcgtga ttcacctggc tgcataggta tgcatgtggt
     4441 taaaatttac cgggtgcgat cgcggcagtt ttccgggtgg tttgttgcca tttttacctg
     4501 tctgctgccg tgatcgcacc gaacgcgttt cggcggtgcg tacaataagt gattatggta
     4561 aatcaccctt ccaggcgttc agcccttctt ccggtttttt tccgttcagg aatttacggg
     4621 cgagacgaat gcgtcttgcc tggtaaatcg catcgtaggc ctgtgactgt cccacaccgt
     4681 tactgatttc actgaccaga tggcgcaggt cggcctgtgt cagtgtgtaa acgtgtttct
     4741 ggatgaggtc acgggctgtc cgctcttcgt atccggcagc gataagtgca gccagcaggc
     4801 gtttgtctgt tttgctcagt cggtggcgac cagattttgt tttgctcacg ttatctcctc
     4861 tctgtttttt ctcctgatgt tatccggtgc tgtttttctg ctgcgaatct tcccttcctg
     4921 atttttataa gggtgattca gatcacaaaa atgaaaattc atatcattga caggaaaaat
     4981 aatgcggcgt aatttatact gtatataaaa ctagtattta tatatacagt atattctgtg
     5041 taaataaagg gagggataaa atgaaagaag aaaaagtatt gcttcaccgt ttcttatttg
     5101 tagttaggaa taagaatggt tgtgaactat catgttcggc agaccttatg ggaacaaggg
     5161 atgatgttta taagtatttc tctgattctg ttagtgagct agatgttgag cttatagatg
     5221 tttcttgcga gagtgaatgg gaagaacatt cgcattgaac taatcaggag attatatgat
     5281 ttctcaaaat gtttgggctc ctcctttctt ttttggtcct tgggttgaac ttgaaaatca
     5341 tactggtgat atcgcgtaca ctgtctattt tgatacattg tctaatgtac ccggtccatt
     5401 tattgctttt atctggtatg cccctaatgg gccaagaaca gttaacgtta ttggtccagc
     5461 atcttatcag atctcttgca agggcgctgg tgttgataaa attaaattta aaagtgtcag
     5521 tgggacaggg cagattattc atgtatacac taccttataa taagagctga ataaatataa
     5581 tcctgcctaa gctatacctt aagcaggatt atatctatta atattatttt gaatatgctt
     5641 ttatgatttc catcactgtt gtttcatcgt tcacaggttg ataataatgg gttggattta
     5701 gtgcagaatg aatgattgca ggttgggata ttacttttcc atcaaaatat gaaatcaggg
     5761 gtttccctat atttttacta gttatttcgt ttagtttttt cttacataaa ctatctgtgt
     5821 ttacttctat tatgacgcct ttgttttttt ctgattggga agggtacatt ttaagtatgc
     5881 aatatttatt cagaatgatg ctgtcaccac caactttgta aacaatgttt ttgtttgttt
     5941 cagtacttga acatcctata agtaaggtca gtatagtact cgatgttatt gactttactg
     6001 atgatttgaa catattaact ccattttatt ttgttaaaat tccaatctat aaatacatat
     6061 acacgtaaaa ataaacaatg cgaacgtttt cacttatatt atccccatga tgtgattgaa
     6121 tggtggttcc atataagaga gggcaattat actatctttc tctcctgaac tgatgggatt
     6181 gcagtttaga gaaaaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6241 ctgctgcctg taaacgaaaa aaccaccctg gcaggtggtt tttcgaaggt tagctaatcc
     6301 tggcagatta cctaaccggg tagctggctt aagtggagca cagataccaa aagatgtcct
     6361 ttcagtgtag ccgctataag gcttcaactt caagagttct gcgtatctaa atccactttt
     6421 tagttaccaa tggctgctgc cagtggcgtt aagtcgtgtc ttaccgggtt ggactcaaga
     6481 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6541 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6601 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taaacggtaa ggtcggaaca
     6661 ggagagcgca cgagggagct tccgggggga aacgcctggt atctttaagt cctgtcgggt
     6721 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6781 ggaaaaacgc cagcggcgcg gccttcttcc tgttcctggt cttttgctca catgtttttt
     6841 ccggtgttat cccctgattc tgtggataac agcatttcgt ttttgagtga gtagataccg
     6901 ctcgccgcag ccgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaaaagcgcc
     6961 tggacgtgca ttttctcctt acgcatctgt gcggcatttc acacccggta tggcgcacgt
     7021 ttcatacaat ccgcactgat gccgcatggt taagccagta tacactccgc tatcgctacg
     7081 tgactggttc agggctgcgc cccgaaaccc gctaaaacac cctgacgcgc cctggcgggc
     7141 ttgtctgctc ccggcatccg ctcacaggca agctgtgacc gtctccggga gctgcatatg
     7201 tcagaggttt tcactgtcat ccccgaagcg cgcgaggcag ctgcggtaaa gctcatcggc
     7261 gtggtcgtga agcgattcac agatatctgc ctgttcatcc gcgtccagtt cgttgagctt
     7321 ctccagcagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc
     7381 ctgtttagtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc
     7441 gatgaaacgc gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact
     7501 ggaacgctgt gagggtaaac aactggcggt atggatgcgg cgggtctgcc tgggtgagcc
     7561 ggttgcccgt tccgggaggc tgccgacact ggcaccgccg ttactgcgtc agctggcagc
     7621 catcgggaat aacctgaacc agacagcccg taaggtgaac agcggacagt ggtcttccgg
     7681 cgaccgggtt caggtggtgg ccgcactgat ggccatcggg gatgagctgc gccggttgcg
     7741 tctggccgtc agggaacagg ggacgcggga tgatagttaa gtttcatgcc cggggaaaag
     7801 gtggtggcag tggtccggtt gattacctgc tgggaaggga gcgtaaccgg gaaggggcaa
     7861 cggtgcttca gggtaatccg gaagaagtac gggaactcat cgatgccacg ccatttgcga
     7921 agaaatacac gtccggtgtg ctgtcgttcg cggagaagga gctgccaccg ggaggacgtg
     7981 aaaaggtgat ggcgagcttt gagcgtgtac tgatgcccgg tctcgaaaaa aatcagtaca
     8041 gcatcctgtg ggtggagcac caggacaagg gacggcttga gctgaatttt gtcattccga
     8101 acatggagct acagaccgga aaacgcctcc agccgtacta cgaccgggca gacaggccga
     8161 gaattgatgc ctggcagacg ctggtgaatc accattacgg gctgcatgac cccaacgccc
     8221 cggagaaccg ccggatactg actctctctg ataacctgcc ggaaacgaaa caggcgcttg
     8281 cagagagcgt cacacgcggg atagacgctc tttatcatgt cggggagata aaaggccgtc
     8341 aggatgtgat tcaggcgctc acagaggcag ggcttgaagt ggtcagggtg acgcgaagca
     8401 gtatcagcat tgcagacccg aacggtggga agaatatcag gctgaaagga gcattttatg
     8461 agcaatcttt tacagacggg cgcggagttc gagaaaaagc tgaaagagag agccgaatct
     8521 acagagacaa tactgaacga cgagttcagg aagctcgaaa aatctgtaag caaggctgtg
     8581 acatcaaacg agacgaaaat caaagacgct atagccctgt tcacagcttc gacagaggaa
     8641 tcactgaaaa agcaccggga aggggtgaaa gaggcgatga tgcagcacag gaaggacgtg
     8701 ttaaagctgg cagggaatac gggcatgatg ttactgggga tggtcatttt cctgtttacc
     8761 gtgagttgcg ggacgctctg gtatcttggc gggatgatac aggcgaacct ggaagatatc
     8821 aggaagcagg aagagacatt gcagaaactg aacgcgaaga catggggcgt ggagttcgta
     8881 caggacggga acaggaaatt tctggtcctt ccgtacggga aatcagcaga ggtgattccc
     8941 catcagggga aggactgggt acaactgatg gagtgacaca gagtgacgga gctggaaaga
     9001 catttgctga acgcattaga gcaactgcaa caggactata tgcagcggct gaacgaatgg
     9061 gagagcgcct tcgtggaatt gcagaaaatg ttttcgctta cgcaacggga caacgcgatg
     9121 ctgaacgagc gggtcatgaa gttgagtcag caggtgcagc acttgagcga gcggacagaa
     9181 cgcttgagcc agttatacag cgagaacagg agataaggga cgaacggctg atacaggaac
     9241 gcgaacatga gttatccctg gagcgtgagc gccagctgga aatacaggaa cggacactgg
     9301 atggcccttc gctgggatgg taatcagaga caggctaaaa tgttgtcagc gcgtttccgg
     9361 tgcatttttt gggataatgg ttggttcggg gcaccgggaa cgcgcttcag tcataaacct
     9421 tgctgaaaac acaggaacgc gttaaaacgc atacgggcgc gattctggtg tgtctttcat
     9481 agtcgtgttt ttgcctctat tgccagatcg cgctcattcc cctgaacttc tgttttcctg
     9541 ctcaaatccc cgtcttcttc gcttccgaaa gtatgaaaaa aatccgtttc agcggtctct
     9601 gactggcaat tcctgcgtga ttcacctggc tgcataggta tgcatgtggt taaaatttac
     9661 cgggtgcgat cgcggcagtt ttccgggtgg tttgttgcca tttttacctg tctgctgccg
     9721 tgatcgcacc gaacgcgttt cggcggtgcg tacaataagt gattatggta aatcaccctt
     9781 ccaggcgttc agcccttctt ccggtttttt tccgttcagg aatttacggg cgagacgaat
     9841 gcgtcttgcc tggtaaatcg catcgtaggc ctgtgactgt cccacaccgt tactgatttc
     9901 actgaccaga tggcgcaggt cggcctgtgt cagtgtgtaa acgtgtttct ggatgaggtc
     9961 acgggctgtc cgctcttcgt atccggcagc gataagtgca gccagcaggc gtttgtctgt
    10021 tttgctcagt cggtggcgac cagattttgt tttgctcacg ttatctcctc tctgtttttt
    10081 ctcctgatgt tatccggtgc tgtttttctg ctgcgaatct tcccttcctg atttttataa
    10141 gggtgattca gatcacaaaa atgaaaattc atatcattga caggaaaaat aatgcggcgt
    10201 aatttatact gtatataaaa actagtattt atatatacag tatattctgt gtaaataaag
    10261 ggagggataa aatgaaagaa gaaaagaggg ataaataaga aaaagaaaag ggaaaaggag
    10321 atggttgtga actatcatgt tcggcagacc ttatgggaac aagggatgat gtttataagt
    10381 atttctctga ttctgttagt gagctagatg ttgagcttat agatgtttct tgcgagagtg
    10441 aatgggaaga acattcgcat tgaactaatc aggagattat atgatttctc aaaatgtttg
    10501 ggctcctcct ttcttttttg gtccttgggt tgaacttgaa aatcatactg gtgatatcgc
    10561 gtacactgtc tattttgata cattgtctaa tgtacccggt ccatttattg cttttatctg
    10621 gtatgcccct aatgggccaa gaacagttaa cgttattggt ccagcatctt atcagatctc
    10681 ttgcaagggc gctggtgttg ataaaattaa atttaaaagt gtcagtggga cagggcagat
    10741 tattcatgta tacactacct tataataaga gctgaataaa tataatcctg cctaagctat
    10801 accttaagca ggattatatc tattaatatt attttgaata tgcttttatg atttccatca
    10861 ctgttgtttc atcgttcaca ggttgataat aatgggttgg atttagtgca gaatgaatga
    10921 ttgcaggttg ggatattact tttccatcaa aatatgaaat caggggtttc cctatatttt
    10981 tactagttat ttcgtttagt tttttcttac ataaactatc tgtgtttact tctattatga
    11041 cgcctttgtt tttttctgat tgggaagggt acattttaag tatgcaatat ttattcagaa
    11101 tgatgctgtc accaccaact ttgtaaacaa tgtttttgtt tgtttcagta cttgaacatc
    11161 ctataagtaa ggtcagtata gtactcgatg ttattgactt tactgatgat ttgaacatat
    11221 taactccatt ttattttgtt aaaattccaa tctataaata catatacacg taaaaataaa
    11281 caatgcgaac gttttcactt atattatccc catgatgtga ttgaatggtg gttccatata
    11341 agagagggca attatactat ctttctctcc tgaactgatg ggattgcagt ttagagaaaa
    11401 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcctgtaaac
    11461 gaaaaaaccg cctgggcagg tggttttcga aggttagcta atcctggcag attacctaac
    11521 cgggtagctg gcttaagtgg agcacagata ccaaaagatg tcctttcagt gtagccgcta
    11581 taaggcttca acttcaagag ttctgcgtat ctaaatccac tttttagtta ccaatggctg
    11641 ctgccagtgg cgttaagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    11701 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    11761 cctacaccga actgagatac ctacagcgtg agcta
//
