LOCUS       NZ_CP095502            16430 bp    DNA     linear   CON 20-JAN-2024
DEFINITION  Escherichia coli strain GN02050 plasmid p2050-3.
ACCESSION   NZ_CP095502
VERSION     NZ_CP095502.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27361453
            Assembly: GCF_026651085.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16430)
  AUTHORS   Bethke,J.H., Ma,H.R., Tsoi,R., Cheng,L., Xiao,M. and You,L.
  TITLE     Vertical and horizontal gene transfer tradeoffs direct plasmid
            fitness
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16430)
  AUTHORS   Bethke,J.H., Ma,H.R., Tsoi,R., Cheng,L., Xiao,M. and You,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2022) Molecular Genetics and Microbiology, Duke
            University, CIEMAS Building 101 Science Drive, Durham, NC 27705, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 6.0.0.47841
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 508x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/20/2024 04:55:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,269
            CDSs (total)                      :: 5,143
            Genes (coding)                    :: 4,917
            CDSs (with protein)               :: 4,917
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 89 of 226
            Pseudo Genes (incomplete)         :: 142 of 226
            Pseudo Genes (internal stop)      :: 46 of 226
            Pseudo Genes (multiple problems)  :: 46 of 226
            Pseudo Genes (short protein)      :: 3 of 226
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095502.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..16430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="GN02050"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2050-3"
                     /geo_loc_name="USA"
                     /lat_lon="36 N 78.9 W"
                     /collection_date="2002"
                     /collected_by="Vance G. Fowler Jr."
                     /genotype="144"
     gene            71..556
                     /locus_tag="MW398_RS00005"
                     /old_locus_tag="MW398_00005"
     CDS             71..556
                     /locus_tag="MW398_RS00005"
                     /old_locus_tag="MW398_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000605869.1"
                     /translation="MIMRCLTHITLVTVIQFIACYLAGWGNAETIFMLFFIVLWQGLFI
                     WLFSQIRKKRNVSDEFKFSKGVWYITIPVSSLLSPLLSLMVFIIGTLYELRRVSGCVSV
                     REWMQSQVNEQTNEDLHLDFDNMDFHRTNPATGLPMSGIGVDIGGNSYGYSSKRYDE"
                     /name="hypothetical protein"
     gene            605..1336
                     /gene="traT"
                     /locus_tag="MW398_RS00010"
                     /old_locus_tag="MW398_00010"
     CDS             605..1336
                     /gene="traT"
                     /locus_tag="MW398_RS00010"
                     /old_locus_tag="MW398_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="WP_000782451.1"
                     /translation="MKMKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIWL
                     EPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKADKM
                     DLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVEDVN
                     YTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNANKVNL
                     KFEEAKPVLEDQLAKSIANIL"
                     /name="traT"
     gene            1539..2276
                     /locus_tag="MW398_RS00015"
                     /old_locus_tag="MW398_00015"
     CDS             1539..2276
                     /locus_tag="MW398_RS00015"
                     /old_locus_tag="MW398_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000199901.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNID
                     TDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILEKISFPEKSGSNLLKPEAVSCNL
                     LSLCVLAPVLCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQGENITWSLSSQSPAD
                     KYDLINKTSRVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIVTEKV
                     SSGRAAGGYHHRHTGYTWSVSVN"
                     /name="hypothetical protein"
     gene            2327..4578
                     /gene="traD"
                     /locus_tag="MW398_RS00020"
                     /old_locus_tag="MW398_00020"
                     /pseudo=""
     CDS             2327..4578
                     /gene="traD"
                     /locus_tag="MW398_RS00020"
                     /old_locus_tag="MW398_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /name="traD"
     gene            4578..9850
                     /gene="traI"
                     /locus_tag="MW398_RS00025"
                     /old_locus_tag="MW398_00025"
                     /pseudo=""
     CDS             4578..9850
                     /gene="traI"
                     /locus_tag="MW398_RS00025"
                     /old_locus_tag="MW398_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /GO_function="GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /name="traI"
     gene            9870..10615
                     /gene="traX"
                     /locus_tag="MW398_RS00030"
                     /old_locus_tag="MW398_00030"
                     /pseudo=""
     CDS             9870..10615
                     /gene="traX"
                     /locus_tag="MW398_RS00030"
                     /old_locus_tag="MW398_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /name="traX"
     gene            10670..11227
                     /locus_tag="MW398_RS00035"
                     /old_locus_tag="MW398_00035"
     CDS             10670..11227
                     /locus_tag="MW398_RS00035"
                     /old_locus_tag="MW398_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="WP_000139329.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLAE
                     KAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIREVL
                     LDEVFQRNIPLSHKKLSRALKAITRSESYLCAMKAGACRYDTEGYVTEHITQEEEQYAQ
                     ARLEKVRRQNRIKDELRAILAE"
                     /name="fertility inhibition protein FinO"
     gene            11379..11582
                     /locus_tag="MW398_RS00040"
                     /old_locus_tag="MW398_00040"
     CDS             11379..11582
                     /locus_tag="MW398_RS00040"
                     /old_locus_tag="MW398_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001336517.1"
                     /translation="MAVSSGLSEHSGIYRYQRVGRHAVRQEIRGFFREAAEAWQNAARL
                     APCEAWRDFALKRASACRCKCR"
                     /name="hypothetical protein"
     gene            12324..12785
                     /locus_tag="MW398_RS00045"
                     /old_locus_tag="MW398_00045"
     CDS             12324..12785
                     /locus_tag="MW398_RS00045"
                     /old_locus_tag="MW398_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="WP_019842128.1"
                     /translation="MKKYIPVVLFVFSCQVFSADIHGRVVRVLDGDTIDVMLSQHPVRV
                     RLVNIDAPEKKQDYGRWSEKIMKSLIAGKTVTVTYFQRDRYGRILGQVYAPDGMNVNHF
                     MVRAGAAWVYEQYNTDPVLPVLQGEARQQKRGLWADSAPVPPWVWRSRK"
                     /name="thermonuclease family protein"
     gene            13054..13272
                     /locus_tag="MW398_RS00050"
                     /old_locus_tag="MW398_00050"
     CDS             13054..13272
                     /locus_tag="MW398_RS00050"
                     /old_locus_tag="MW398_00050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150739.1"
                     /translation="MIMRLFFIRDALNASEHIIILNCTGWPDLNNESAVMQFARVARKS
                     GKQLIVAVREQDMKKMEAESGRIIKIH"
                     /name="hypothetical protein"
     gene            13425..13862
                     /locus_tag="MW398_RS00055"
                     /old_locus_tag="MW398_00055"
     CDS             13425..13862
                     /locus_tag="MW398_RS00055"
                     /old_locus_tag="MW398_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000872609.1"
                     /translation="MKYDDVSELMIKCEHNILIVGNTYSRVDNFFKGYSVPDSKYYDFT
                     QINSDSDAERNDHAVSFIRDAMNSEEHIIIFNCVGWPDLGGGSAVSQFAMMARKFGKQL
                     IVAVSEKDAIKLKDNFNIIGTLSYGKENFIAMSHTKSEFCS"
                     /name="hypothetical protein"
     gene            14061..14210
                     /locus_tag="MW398_RS00060"
                     /old_locus_tag="MW398_00060"
     CDS             14061..14210
                     /locus_tag="MW398_RS00060"
                     /old_locus_tag="MW398_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="WP_001336447.1"
                     /translation="MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAY
                     EARK"
                     /name="Hok/Gef family protein"
     gene            14495..14752
                     /locus_tag="MW398_RS00065"
                     /old_locus_tag="MW398_00065"
     CDS             14495..14752
                     /locus_tag="MW398_RS00065"
                     /old_locus_tag="MW398_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="WP_000084404.1"
                     /translation="MSQTVNAETSSSGKKRPYRRGNPVPARERQKASLARRSATHKAFH
                     AVIQLRLKEKLSELADEDGITQAQMLEWLIESEVKRRKSL"
                     /name="replication regulatory protein RepA"
     gene            14988..15062
                     /gene="tap"
                     /locus_tag="MW398_RS00070"
                     /old_locus_tag="MW398_00070"
     CDS             14988..15062
                     /gene="tap"
                     /locus_tag="MW398_RS00070"
                     /old_locus_tag="MW398_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]; GO:0031556 - transcriptional attenuation by ribosome
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="WP_001365705.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
                     /name="tap"
     gene            15055..15928
                     /gene="repA"
                     /locus_tag="MW398_RS00075"
                     /old_locus_tag="MW398_00075"
                     /pseudo=""
     CDS             15055..15928
                     /gene="repA"
                     /locus_tag="MW398_RS00075"
                     /old_locus_tag="MW398_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311752.1"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator RepA"
                     /name="repA"
     Typing          15086..15912
                     /name="IncFIA"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.276"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          4579..9410
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.883"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9541..9860
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="85.981"
                     /coverage="100.0"
                     /evalue="2.01e-51"
     Typing          3128..4575
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="93.375"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tccacaggaa cttatgaaaa gggcaaagga atatcaggat aagtataagc aataatgctg
       61 tttttgggag atgataatga gatgtttaac ccatattact ctggtaacgg tgatacagtt
      121 tatcgcctgt tatctggctg gttgggggaa tgccgaaaca atttttatgc tgttcttcat
      181 agtactatgg cagggactgt ttatatggtt gttctctcaa atacgaaaaa aacggaatgt
      241 gtcggatgag tttaagttta gtaaaggcgt ttggtatata acgataccag tatcttcctt
      301 actctctcca ttattaagcc tgatggtttt tatcattggt acactatacg aattaaggcg
      361 agttagtggt tgtgtaagcg taagagaatg gatgcaatct caggtaaatg aacaaactaa
      421 tgaagatttg catctggatt ttgataatat ggattttcat cgtactaatc ctgcaacagg
      481 gttacctatg tcaggtattg gtgtggatat cggtggtaat tcatatggtt atagttcaaa
      541 acgatatgat gagtgaatct taatttgtat attatgacgt tttattcaat atgaaggaac
      601 attgatgaaa atgaaaaaat tgatgatggt tgcactggtc agttccactc tggccctttc
      661 agggtgtggt gcgatgagca cagcaatcaa gaagcgtaac cttgaggtga agactcagat
      721 gagtgagacc atctggcttg aacccgccag cgaacgcacg gtatttctgc agatcaaaaa
      781 cacgtctgat aaagacatga gtgggctgca gggcaaaatt gctgatgctg tgaaagcaaa
      841 aggatatcag gtggtgactt ctccggataa agcctactac tggattcagg cgaatgtgct
      901 gaaggccgat aagatggatc tgcgggagtc tcagggatgg ctgaaccgtg gttatgaagg
      961 cgcagcagtt ggtgcagcgt taggtgccgg tattaccggc tataactcaa attctgccgg
     1021 tgccacactc ggtgtaggcc ttgctgctgg tctggtgggt atggctgcag atgcgatggt
     1081 ggaagatgtg aactatacca tgatcacgga tgtacagatt gcagagcgta ctaaggcaac
     1141 ggtgacaacg gataatgttg ccgccctgcg tcagggcaca tccggtgcga aaattcagac
     1201 cagtactgaa acgggtaacc agcataaata ccagacccgt gtggtctcaa atgcgaacaa
     1261 ggttaacctg aaatttgaag aggcgaagcc tgttctcgaa gaccagctgg ccaaatcaat
     1321 cgcaaatatt ctctgaacct cgtcaggagg ctgatctgac cggcctcctt gctgacgcct
     1381 cgcgttgctc gttgtccaac cccgggatgt aacaaaataa attttgtcac ctcccggcgt
     1441 ttccgaaata aatatgaaaa cactgagcgg ttgtgcaaat tctttgtgcg accgtgaagt
     1501 gttgccggcg aagccgggat cttccggaga aagtcattat gacatcagac aatatcgctg
     1561 aatatgttgg tgagaataat caatttactg agaagcctgt aactgaatac cccccggtga
     1621 tacaggataa tacagtgacc actaaagact atctgtacag caataatatt gatactgacc
     1681 ttttagtggt tatcggaggg cttattgtta tattgtttct catgatgctt atcatgtact
     1741 ttattaattc tctcaggcat aaggaattaa ctgaaatact ggaaaaaata tcttttcctg
     1801 aaaaatccgg gagcaatttg cttaaaccgg aggcggtcag ctgtaatttg ttgtccctgt
     1861 gtgtgctggc accggtactc tgtacaccca gaaagctggc agaagtctcc agggagatca
     1921 tcgtggcgag tgcgcaggaa attgcatgga cgccggggag cagtcgtatt gatggtcatc
     1981 agggcgaaaa tattacctgg tctctgtcat cgcagtctcc tgccgataag tatgatctca
     2041 tcaataaaac ctcccgtgtt tctcctgaca gtggccgttt ttatgaaccg gacacagttc
     2101 tggatcaccg tgaacgggta cgccttttta ttcagatcca gtttccgcac gggagtgact
     2161 ggtctgaagt attgcgactg actgacattg ttacagagaa ggtctcttcc ggcagggctg
     2221 caggcgggta tcaccaccgg catacagggt acacctggtc tgtttctgtg aattaacccc
     2281 acatcatcct tttctgccct gttccagatt ttcccggagt ttcatcatga gttttaacgc
     2341 aaaggatatg acccagggcg gtcagattgc gtccatgcgt atccgcatgt tcagccagat
     2401 cgccaatatc atgctttact gcctgtttat ttttttctgg atactcgttg gtctgatttt
     2461 atgggtaaaa ataagctggc agacgtttgt gaacggctgt atttactggt ggtgtacgtc
     2521 acctggaagg catgcgggat ttaataaagt cccagccagg tatatgagat ccagtattac
     2581 ggcaaaacct tccggatgaa cgctgcccag gtactgcatg ataaatatat gatctggtgc
     2641 ggagagcagc tatggtctgc attcgttctg gccagtctgt cgtggccctg gttatttgcc
     2701 tgatcacctt ctttgttgtc tcctggattc tggggcatca ggggaaacag cagagtgaaa
     2761 atgaaggtgc acaggcgggc gtcagctgac ggataatccg aaagacgtgg cccggatgct
     2821 gaaaaaagac ggcaaggact ccgatatccg gattggcgac ctgccgatta tccgggattc
     2881 cgaaatccag aacttctgtc tgcacggcac ggtcggggcc ggtaagtcgg aggttatccg
     2941 tcgtctggcc aactactgcc cgtcagcgcg gtgatatggt ggtgatttat gaccgttcag
     3001 gggaatttgt taaaagttac tatgatccct ccatcgataa aatcctgaat ccgctggatg
     3061 cccgctgtgc cgcatgggat ttgtggaagg agtgcctgac acagccggat tttgataata
     3121 ccgcgaacta cgctgatccc tatgggtaca aaagaggacc cgttctggca gggttcagga
     3181 cgtaccattt ttgcggaagc ggcgtacctg atgcgtaatg accccaaccg cagctacagc
     3241 aaactggtgg acacactgct ttccatcaaa attgaaaaac tgcgtacctt cctgcgtaat
     3301 tcaccggcag ccaacctggt ggaagagaaa attgagaaaa cggcgatttc catccgtgct
     3361 gtgctgacca actacgtgaa agccatccgt tacctgcagg ggattgagca taacggtgag
     3421 tcctttacca tccgtgactg gatgcgtggt gtccgggaag atcagaaaaa cggctggctg
     3481 tttatttcgt cgaacgccga cacccatgcc tccctgaagc cggtgatctc catgtggctg
     3541 tccattgcca ttcgtggtct gctggcaatg ggagaaaacc gtaaccgtcg tgtgtggttt
     3601 ttctgtgacg agttacccac gttacacaaa ctgccggacc tggtggagat cctgccggaa
     3661 gcccgtaagt tcggtggctg ttatgtgttt ggtatccagt cctatgccca gctggaagat
     3721 atctacggtg agaaagcggc ggccacgctg tttgacgtca tgaacacccg tgcctttttc
     3781 cgttctccca gccataagat tgcagagttc gctgccggtg aaattggtga gaaagagcac
     3841 ctgaaagcca gcgagcagta ttcctacggt gctgacccgg tacgtgacgg ggtatcgacc
     3901 ggtaaggata tggagcgtca gacgctggtc agttattccg acattcagtc tctgccggat
     3961 ctgacctgct atgtcactct gcccggaccg tatccggcag tcaaactctc tctgaaatat
     4021 caggcacgac cgaaggtcgc tccggagttt attccgcgtg acatcaaccc ggaaatggag
     4081 aaccgtctga gtgccgtact tgctgcaagg gaagcagaag gtcgccagat ggccagcctc
     4141 ttcgaaccgg aggttgcctc cggagaaggc gtgactcagg ctgaacagcc acaacagcca
     4201 caacagccac aacagccaca acagccacaa cagccacaac agccacaaca gccacaacag
     4261 ccacaacagc cacaacagcc acaacagcca caacagccag tgtcttctgt catcaatgat
     4321 aagaagtcag atgcaggtgt gagtgttccg gcagggggga tcgagcagga gctgaaaatg
     4381 aaaccggaag aagagatgga acagcaactg ccacccggga tcagtgaatc cggtgaagtg
     4441 gtggatatgg ccgcttatga ggcatggcaa caggaaaatc atccggacat ccagcagcag
     4501 atgcagcgtc gtgaagaggt gaacattaat gtgcaccggg agcgtgggga ggatgttgag
     4561 ccgggagatg atttctgatg atgagtatcg cgcaggtcag atcggccgga agtgccggta
     4621 actattatac cgacaaggat aattactatg tactgggcag catgggagaa cgctgggccg
     4681 gcaggggggc tgaacagctg ggactgcagg gcagtgtcga taaggatgtt tttacccgtc
     4741 ttctggaggg aaggctgccg gacggagcgg atctaagccg catgcaggat ggcagtaata
     4801 agcatcgtcc cggctacgac ctgaccttct ccgcccccaa aagtgtctcc atgatggcca
     4861 tgttaggtgg cgataagcgc ctgattgatg cacataacca ggccgtggac tttgctgttc
     4921 gtcaggtgga ggcgctggcc tccacacggg tgatgacgga cggacagtca gaaacggtgc
     4981 tgaccggtaa tctggtgatg gcactgttta accacgacac cagtcgcgat caggaaccac
     5041 agttacacac gcatgcggtg gtggctaatg tcacgcagca taacggtgag tggaagacac
     5101 tgagcagtga caaagtgggg aaaacggggt tcattgagaa tgtgtacgct aatcagattg
     5161 cctttggcag gctctaccgg gaaaaactga aagagcaggt tgaggcgctg ggctatgaaa
     5221 ccgaagtggt gggtaagcac ggtatgtggg agatgccggg cgtaccggtg gaggcctttt
     5281 ccggacgctc acaggctatc cgggaggccg tcggggaaga cgcctcgctg aaatcccggg
     5341 atgtggcggc actggatacg cgtaaatcca aacagcacgt cgatccggaa atcagaatgg
     5401 ctgaatggat gcagacgctg aaggaaaccg ggttcgacat ccgggcatat cgtgacgcgg
     5461 cggatcagcg tgcggagacc cgcacgcagg cgcccggggc tgtttcacag gaggggccgg
     5521 atgtgcagca ggcggtgaca caggccattg ccggattaag tgaacgaaaa gtgcagttta
     5581 cgtacacgga cgtgctggcc agaacggtcg gtatactgcc gccggaaaat ggtgtgattg
     5641 aacgggcacg cgccggtatc gatgaggcca tcagccgtga gcagcttatc cccctcgacc
     5701 gtgagaaggg gctgttcaca tcaggaattc atgtgctcga tgagctgtcc gtccgggcac
     5761 tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca tccggagaaa agtgtccccc
     5821 ggacggctgg ttacagcgat gcggtcagtg ttctggcaca ggaccgtccg tcgctggcca
     5881 ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg ggtggctgaa ctggtcatga
     5941 tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc tgaccgtcgc tcgcagatga
     6001 acctgaagca ggatgaacgg ctgtccggtg agctgataac cggacgtcgt cagctgcagg
     6061 agggcatggt cttcacgccg ggcagtactg ttatcgtcga ccagggcgaa aaactctccc
     6121 tgaaagagac gttaaccctg ctggacggtg ccgcacgtca taacgtacag gtcctgataa
     6181 ccgacagcgg gcagcgaacc ggtacaggca gtgcgctgat ggccatgaag gatgccgggg
     6241 tgaacacgta tcgctggcag ggcggagaac agcgaccggc aaccatcatc agtgaaccgg
     6301 accggaatgt ccgctatgac cggctggccg gagattttgc ggccagcgtg aaagccggag
     6361 aagagagcgt ggcacaggtc agcggggtac gggaacaggc catactgaca caggccattc
     6421 gcagtgagct gaaaacacag ggcgtgctcg gacacccgga ggtgaccatg accgccctgt
     6481 caccggtctg gctggacagc cggagccgtt atctgcggga tatgtaccgc ccggggatgg
     6541 tgatggagca gtggaacccg gagacacgca gtcatgaccg ctatgttatc gaccgggtga
     6601 cggcgcagag tcacagcctg accctgcggg atgcgcaggg cgaaacgcag gtggtgcgta
     6661 tttcctccct ggacagcagc tggtcgctgt tccggccgga aaaaatgccg gtggcagacg
     6721 gcgagcgact gagggtgaca gggaaaattc ccggactccg cgtctccggc ggtgaccgcc
     6781 tgcaggtggc atccgtcagt gaagatgcga tgacggttgt tgtgccgggg cgggctgaac
     6841 cggcctccct gcctgtgagc gattcaccgt tcacggcact gaagctggag aacggctggg
     6901 tggaaacgcc cgggcattcc gtcagtgaca gtgcgacggt gtttgcctcc gtcacacaga
     6961 tggcgatgga taacgcgacc ctgaacggtc tggcccgcag cggtcgtgat gtccggctgt
     7021 attcctcact ggatgaaacc cgtactgcgg aaaaacttgc ccgccatccc tcctttacgg
     7081 tggtttctga gcagataaag gcgcgtgccg gtgagacatc gctggaaacc gctatcagtc
     7141 tgcagaaaac cgggctgcac acgccggcac agcaggccat tcatctggcc cttcctgtgc
     7201 tggaaagtaa aaacctggcc ttcagcatgg tggacctgct gacagaggcg aagtcgtttg
     7261 ctgcagaagg aaccggtttt actgaactgg gaggggaaat caatgcgcag ataaaacgcg
     7321 gtgacttact gtatgtggat gtggcaaaag gctatggcac aggcctgctg gtttcccgtg
     7381 cgtcgtatga ggcagaaaaa agtattcttc gccatattct cgaaggtaag gaggcggtca
     7441 cgccgctgat ggagagagta cccggcgaac tcatggagac gttaacgtcg ggacagcgtg
     7501 ccgccacccg tatgatactg gaaacgtccg accgtttcac ggtggtacag ggttatgccg
     7561 gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc cgtgaacatg ctgccggcga
     7621 gtgagcgtcc ccgagttgtg gggctggggc ccacgcaccg tgcggtcggt gagatgcgca
     7681 gcgccggcgt ggatgcacag acgctggcgt cctttctgca tgacacgcag ctgcagcagc
     7741 gcagcggaga aacgccggat ttcagcaaca cgctgttcct gctcgatgag agctcaatgg
     7801 tgggcaatac cgacatggca cgggcatacg ccctgattgc ggccggtggc ggtcgtgccg
     7861 tggccagtgg tgacacggac cagctgcagg ccatcgcgcc cggtcagcct ttccgtctcc
     7921 agcagacgcg cagtgctgcc gatgtggtca tcatgaagga gattgtgcgt cagacgccgg
     7981 aactgcggga ggcggtatac agcctgatta accgggatgt ggaaagggca ctgtccgggc
     8041 ttgagagtgt gaaaccgtct caggttcccc gtcaggaggg cgcatgggca ccggagcact
     8101 ccgtgacgga gttcagtcac agccaggaag cgaaactggc agaagcgcag cagaaggcga
     8161 tgctgaaagg tgagactttt ccggatgtcc ccatgacact gtatgaagcc attgtccgcg
     8221 actataccgg caggacaccg gaagcacggg agcagacgct gattgtcacg cacctgaatg
     8281 aggaccggcg cgtactgaac agcatgattc atgatgcacg ggaaaaggcc ggtgagctgg
     8341 ggaaagagca ggtcatggtg cctgtcctga acacagcgaa tatacgtgac ggggagctgc
     8401 gtcgtctctc cacctgggag aataacccgg atgcccttgc cctggtggat agtgtgtatc
     8461 accggattgc cggtatcagc aaggatgacg ggctgataac cctggaggat gcggaaggta
     8521 acacgcggct gatttcgccc cgggaggcgg tggctgaagg tgtcacactg tacaccccgg
     8581 acaaaatccg ggtggggacc ggcgaccgga tgcgcttcac gaagagtgac cgggagcgcg
     8641 gttatgtggc caacagcgtc tggacggtga cagcggtttc cggtgacagt gtcacgctgt
     8701 cagacggaca gcagacccgg gtgattcgcc ccggccagga gcgggcagag caacatattg
     8761 acctggctta tgccatcacc gcccacggtg cgcagggggc gagtgaaacc tttgccatcg
     8821 cgcttgaagg cacggaaggt aaccggaaac tgatggccgg ctttgagtca gcctacgtgg
     8881 ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga taaccgtcag ggctggacgg
     8941 atgccattaa caatgccgta cagaaaggaa ctgcccacga tgtgctggag ccgaaaccgg
     9001 accgggaggt catgaatgcg cagcggctgt tcagtacggc acgggaactg cgggacgtgg
     9061 cggcaggccg tgccgttctc cgtcaggcag ggctggccgg gggagacagc cctgcacggt
     9121 ttattgcacc gggacgtaaa tatccgcagc cgtatgtggc actgccggcg tttgaccgta
     9181 acggcaagtc agctggtatc tggctgaacc cactgaccac ggatgacgga aacgggctgc
     9241 ggggattcag tggtgaagga cgggtgaaag gcagcgggga tgcgcagttc gtggccctgc
     9301 agggcagccg taacggagag agcctgctgg ctgataatat gcaggatggc gtccggattg
     9361 cccgtgataa tcctgacagc ggtgtggtgg tgagaattgc cggtgaaggt ccgtccgtgg
     9421 aatcccggtg ccatcaccgg tggccgcgtg tggggggata tcccggacta acagcgtcca
     9481 gccgggggcc ggaaatggcg aaccggtcac ggcagaggta ctggcacagc ggcaggctga
     9541 agaggccatc cgccgtgaaa cggaacgccg cgcagatgaa attgtcagga aaatggcaga
     9601 gaacaaacct gacctgccgg atggcaaaac agagcaggct gtcagggaaa ttgccgggca
     9661 ggagcgtgac cgggctgaca taactgaacg ggaagccgcg ctgccggaga gtgtgctgcg
     9721 tgaatcacaa cgggagcagg aagcggtccg ggaggttgcc cgggaaaatc tgctgcagga
     9781 gcgactgcag cagatggagc gggatatggt tcgtgacctg cagaagagaa aaccctgggg
     9841 cggagactga tacaggaaga taaacgctga tgacaacgga taacacgaac acgacacgta
     9901 acgattcact ggctgcacgg accgatacct ggtttcagtc atttctggtc tggtcccccg
     9961 gacagcggga tatcatcaaa acggtggcac tggtgctgat ggtactggac cacatcaatc
    10021 tgatattcca gctgaagcag gaatggatgt ttctggccgg gcgaggagcc tttccgctgt
    10081 ttgccctggt gtgggggctg aatctgtccc gtcatgcgca tatcaggcag tcagccatta
    10141 accggttatg gggctgggca gttttttccc agttcgcata ttatctggcc ggatttccct
    10201 ggtatgaggg gaatatcctg tttgcctttg cagtggcggc acaggtgctg acatggtgtg
    10261 aaacgcgcag tgggtggcgt acagcagcgg ccattcttct gatggccctg tgggggcctt
    10321 tgtccggcac cagttacggc attgccgggc tgctgatgct ggcagtcagc caccggctgt
    10381 accgggcgga agacagagcg gaacgtctgg cgctggtcgc ctgcctgctg gctgttattc
    10441 cggcgcttaa ccttgccacc agtgatgcag cggcggtggc tggtcttgtg atgacggtgc
    10501 tgaccgttgg tctggtgtcc tgtgcaggaa aatcattacc ccgtttctgg cccggggatt
    10561 tttcccggta ttctacgcct gtcatctggc tgtgctgggc gttctggcgc tgtgacgggt
    10621 gtggtatctt tgaccgtaac aggatgattc gtcagaggca gaacacagca tgacagagca
    10681 gaagcgaccg gtactgacac tgaagcggaa aacggaaggg gagacgcctg tccgcaggcg
    10741 gaaaaccatc atcaatgtca ccacgccacc aaagtggaag gtgaaaaagc agaagctggc
    10801 cgagaaagca gcccgggaag cggagctggc ggcaaaaaaa gcgcaggcca gacaggcgct
    10861 gtccatttat ctgacgctgc cctcgctgga tgaggcggtg aacactctga aaccctggtg
    10921 gccgggattg tttgacggta acacgcccag gcttcttgcc tgtggtatcc gggaggtttt
    10981 actggatgag gtgtttcagc ggaatatccc gctctctcat aaaaaactca gccgggcgct
    11041 gaaagctatc acccgttcgg aaagttatct gtgtgcaatg aaagccggcg cctgccgcta
    11101 tgatacggaa ggctatgtga cagaacatat cacacaggaa gaagaacagt atgcgcaggc
    11161 gcgcctggaa aaggtgcgca gacagaaccg gatcaaggat gagctgcggg cgatcctggc
    11221 ggaataaaag agccctctgt atgacagagg gcaacagcga gaaaaaatcg ggaaaacaga
    11281 aatccagagt tttgatggag aaaacaccca accgttcagg gatgtttcag aatgtttctg
    11341 acataatccg ggagccgggt caagaggggg gataaaaaat ggctgtatcc agtggattat
    11401 ctgagcacag cggtatttat cgctatcagc gcgtggggcg tcatgccgtc cgtcaggaga
    11461 ttcgtggttt tttcagggaa gccgctgagg cctggcagaa tgcggcccgg ctggcaccct
    11521 gtgaagcatg gcgggatttt gccctgaaga gggcatctgc atgccggtgt aagtgccgat
    11581 aaaaataacc cccgtcgcgg agctccgggg gatcaggctt atatggcaga tcgttattat
    11641 tatagtggtg taagaaggag ttgccctgtc tttcaaacgg acatcacaga gttaacatat
    11701 ttcacatccc gggtaagcca gcagggataa aaaatgtgat gaatttcaag aagcagaagg
    11761 tgacagtagc atgcataaca gtacggttat gtccggtgaa tctgacagac agccgggctg
    11821 tattcgcggc aggataaaga aaaaccggga gatgactctc ccggtccgtc ttatcgctaa
    11881 tcccctgagc agggcatgta tgacttatct atgaccgtta tattcagcgc cacatccggt
    11941 agcctgcgat ataacacagt tatcctatca gtaagagcag gtatgatgaa tacacggaga
    12001 ttaaaaatat aatctccgtc acatgttacc cccggaaacc ggtaacgccg tcacgggaaa
    12061 tacgtgacag aaaaagcagc ctccgttact ccaggtatca gaaggctgaa taatgcatga
    12121 gactttgttt cttatgtgtc tggtcaggct ggctgaccag cgtttttagt aacacatagc
    12181 catatgtgta ttaagccacc tcatcttatt ttgtcgaatt gatcacaatt caacggttta
    12241 tttttggcgt gatacgtgag ccgttattca tcccggcatg gaattcatgc aatcacgaaa
    12301 agtaatcagt gacggagagt aaaatgaaga aatatatacc agtggtatta tttgtttttt
    12361 catgtcaggt attttctgct gatattcatg gacgggttgt gcgtgttctg gatggtgata
    12421 ccattgacgt catgttatct cagcatccgg tgcgtgtccg tcttgttaat attgatgcac
    12481 ctgaaaagaa acaggactat ggccggtggt ctgaaaaaat aatgaaatct ctgattgcgg
    12541 gaaaaacggt tactgtcaca tattttcagc gagatcgtta tggtcgtatt ctcgggcagg
    12601 tttatgcacc agacggaatg aatgttaatc attttatggt ccgtgcaggc gctgcatggg
    12661 tttatgagca gtacaacacc gatcctgttc ttcccgtcct gcagggagaa gcacgacagc
    12721 agaaacgtgg tctgtgggca gacagcgctc ctgttccgcc ctgggtatgg cgctcacgga
    12781 aataatgata tttctgtggc tattgagttc tcattgaaat gcgatattta aaagcatgaa
    12841 gcatattaca ttattaactt atttctctgt taatgaaagg tgaacaaatg aactatcagg
    12901 cagtatcaga attaatcaca tcgtctaatc ataatgttct tatagtgtgg gactcagctt
    12961 ctgaagttga tggtttttta aataaactga atatcactga ttataagtat tatgatttca
    13021 gccagattta cagttgttct gacagaacat taaatgatta tgcggttatt ttttatcagg
    13081 gatgcactga acgccagtga gcatattatt attttaaatt gtacaggatg gcctgatttg
    13141 aataatgaat ctgccgtcat gcagtttgcc agagtggcac gaaaatcagg aaaacaactg
    13201 atagtcgcag taagagaaca ggatatgaaa aaaatggaag cagagtctgg aaggatcata
    13261 aaaatccact gacgccttcg cgttgctcag ttgtccaacc ccggggacgg gaaaaagcaa
    13321 gttttccccg tccccggcgt ttctgaataa aaaccgggaa atggtggcac agggggaaag
    13381 ccggaaccac ctgtatccag attttatctc ataatggtga atatatgaaa tatgatgatg
    13441 tgtcagaatt gatgattaaa tgtgaacaca acattcttat tgttggtaat acttactccc
    13501 gagttgataa tttttttaag gggtattctg taccagattc taaatattat gatttcactc
    13561 agattaacag tgattcagat gcagagagaa atgatcatgc cgtctccttt atcagggatg
    13621 caatgaatag tgaagagcat ataattattt ttaattgtgt tgggtggcct gatttaggtg
    13681 gtggatcagc agtatcgcag tttgcaatga tggccagaaa atttggtaaa caacttattg
    13741 tcgctgtaag tgaaaaggat gccataaaat taaaagacaa ctttaatatt attggaacgt
    13801 tgtcatatgg taaagaaaat tttattgcca tgagccatac gaaaagcgag ttctgtagtt
    13861 aaaagcggga aatggaggta atggtcagca acagggaatg tggtattatc gcggcgggtg
    13921 tctgagcctt tctggttcag gcaagacgca ggtaccagaa atgcgaagac cccacttgtt
    13981 aatccattaa ctcgtgaggc ctgcatgaag tacgtagcac tactgattgt agcctcttcc
    14041 ttgcagagag gtcaaagttt atgacgaaat atacccttat cgggttgctc gccgtgtgcg
    14101 ccacggtgtt gtgtttttca ctgatattca gggaacggtt atgtgagctg aatattcaca
    14161 ggggaaatac agtggtgcag gtaactctgg cctacgaagc acggaagtaa gctgccgggc
    14221 gggggcggaa gtccccgctt tccggaagtg tggggtattt caggggcaga cacccgacat
    14281 gccagaaacc gccggtcccg cccggggccg gcacacaggt taaggcattt cctgtttttt
    14341 cagccatttc gcaacaaaaa tcacattaaa caacagtaat cagacatgat ttgtgcgcca
    14401 acacagatct tcgtcacaat tctcaagtcg ctgatttcaa aaaactgtag tatcctctgc
    14461 gaaacgatcc ctgtctgaga agagaggagg cgagatgtcg cagacagtaa atgcagagac
    14521 ttcctcatcg ggaaaaaaac gaccttacag aaggggtaat cctgttcctg cgagagaacg
    14581 acaaaaagcg tctcttgcaa gaaggagtgc cactcataaa gcgtttcatg ccgtcattca
    14641 gcttcgactg aaagaaaagt taagtgagct tgctgatgaa gacgggatca ctcaggcaca
    14701 gatgcttgag tggctgatag agtcagaggt taagcgcaga aaatctttgt gaatatttgc
    14761 gtttcttgct gtttcagtga tgagatgtta gattgctgat cgttttaagg aattttgtgg
    14821 ctggccacgc cgtaaggtgg cagggaactg gttctgatgt ggatttacag gagccagaaa
    14881 agcaaaaacc ccgataatct tcttcaactt tggcgagtac gaaaagatta ccggggccca
    14941 cttaaaccgt atagccaaca attcagctat gcggggagta tagttatatg cccggaaaag
    15001 ttcaagactt ctttctgtgc tcgctccttc tgcgcattgt aagtgcagga tggtgtgact
    15061 gatcttcacc aaacgtatta ccgccaggta aagaacccga atccggtgtt tacaccccgt
    15121 gaaggtgcag gaacgctgaa gttctgcgaa aaactgatgg aaaaggcggt gggcttcact
    15181 tcccgttttg atttcgccat tcatgtggcg catgcccgtt cgcgtggtct gcgtcgacgc
    15241 atgccaccag tgctgcgtcg acgggctatt gatgcgctcc tgcaggggct gtgtttccac
    15301 tatgacccgc tggccaaccg cgtccagtgc tccatcacca cgctggccat tgagtgcgga
    15361 ctggcgacgg agtctgctgc cggaaaactc tccatcaccc gtgccacccg ggccctgacg
    15421 ttcctgtcag agctgggact gattacctac cagacggaat atgacccgct tatcgggtgc
    15481 tacattccga ccgatatcac gttcacatct gcactgtttg ctgccctcga tgtatcagag
    15541 gaggcagtgg ccgccgcgcg ccgcagccgt gtggtatggg aaaacaaaca acgcaaaaag
    15601 caggggctgg ataccctggg catggatgaa ctgatagcga aagcctggcg ttttgttcgt
    15661 gagcgttttc gcagttatca gacaggagct taagtcccgg ggaataaagc gtgcccgtgc
    15721 gcgtcgtgat gcggacaggg aacgtcagga tattgtcacc ctggtgaaac ggcagctgac
    15781 gcgcgaaatc gcggaagggc gcttcactgc caatcgtgag gcggtaaaac gcgaagttga
    15841 gcgtcgtgtg aaggagcgca tgattctgtc acgtaaccgt aattacagcc ggctggccac
    15901 agcttcccct gaaagtgacc tcctctgaat aatccggccc gcgctggagg cattcgcacg
    15961 cctgaagccc gtcgatgcac aaaaaaacag caccacatac aaaaacaacc tcatcatcca
    16021 gcttctggtg catccggccc cccctgtttt tcgatacaaa acacgcctca cagacgggga
    16081 attttgctta tccacattaa actgcaaggg acttccccat aaggttacaa ccgtttcatg
    16141 tcataaagcg ccagccgcca gtcttacagg gtgcaatgta tcttttaaac acctgtttat
    16201 atctccttta aactacttaa ttacattcat ttaaaaagaa aacctgttca ctgcctgtcc
    16261 tgtggacaga cagatatgca cctcccaccg caagcggcgg gccccgaccg gagccacttt
    16321 agttacaaca cacaaaaaca acctccagaa aaaaaccccc ggaccagcgc agaactggag
    16381 ccgcaaagcc cctccctcat aactgaaaag cggcccccgc cccggccgaa
//
