LOCUS       NZ_CP095453             8910 bp    DNA     circular CON 05-JUN-2024
DEFINITION  Escherichia coli strain LS45 plasmid pLS45-5, complete sequence.
ACCESSION   NZ_CP095453
VERSION     NZ_CP095453.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27413057
            Assembly: GCF_023022925.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8910)
  AUTHORS   Ma,J.
  TITLE     The mcr-1, tet(X) and blaNDM postive Escherichia coli strains
            isolated from pig and chicken in Zhejiang
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8910)
  AUTHORS   Ma,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2022) Institute of Agro-Product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198
            Shiqiao Road, Hangzhou, China 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. June-2021
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023022925.1-RS_2024_06_05
            Annotation Date                   :: 06/05/2024 03:47:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,413
            CDSs (total)                      :: 5,298
            Genes (coding)                    :: 4,924
            CDSs (with protein)               :: 4,924
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 374
            CDSs (without protein)            :: 374
            Pseudo Genes (ambiguous residues) :: 0 of 374
            Pseudo Genes (frameshifted)       :: 225 of 374
            Pseudo Genes (incomplete)         :: 174 of 374
            Pseudo Genes (internal stop)      :: 55 of 374
            Pseudo Genes (multiple problems)  :: 65 of 374
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095453.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LS45"
                     /host="chicken"
                     /db_xref="taxon:562"
                     /plasmid="pLS45-5"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2020"
     gene            complement(362..595)
                     /locus_tag="MWN28_RS26945"
                     /old_locus_tag="MWN28_26955"
     CDS             complement(362..595)
                     /locus_tag="MWN28_RS26945"
                     /old_locus_tag="MWN28_26955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029400396.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTEHLSQLYSESRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(602..1120)
                     /locus_tag="MWN28_RS26950"
                     /old_locus_tag="MWN28_26960"
     CDS             complement(602..1120)
                     /locus_tag="MWN28_RS26950"
                     /old_locus_tag="MWN28_26960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_029400393.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKVLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMRQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1158..1808)
                     /locus_tag="MWN28_RS26955"
                     /old_locus_tag="MWN28_26965"
                     /pseudo=""
     CDS             complement(<1158..1808)
                     /locus_tag="MWN28_RS26955"
                     /old_locus_tag="MWN28_26965"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1798..2121)
                     /locus_tag="MWN28_RS26960"
                     /old_locus_tag="MWN28_26970"
     CDS             complement(1798..2121)
                     /locus_tag="MWN28_RS26960"
                     /old_locus_tag="MWN28_26970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955996.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2185..2376
                     /locus_tag="MWN28_RS26965"
                     /old_locus_tag="MWN28_26975"
     CDS             2185..2376
                     /locus_tag="MWN28_RS26965"
                     /old_locus_tag="MWN28_26975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3046..3204
                     /locus_tag="MWN28_RS26970"
                     /old_locus_tag="MWN28_26980"
     CDS             3046..3204
                     /locus_tag="MWN28_RS26970"
                     /old_locus_tag="MWN28_26980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001354639.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAAVGNLVSRAMMA
                     QMVQQSS"
                     /name="hypothetical protein"
     gene            3700..3927
                     /locus_tag="MWN28_RS26975"
                     /old_locus_tag="MWN28_26985"
     CDS             3700..3927
                     /locus_tag="MWN28_RS26975"
                     /old_locus_tag="MWN28_26985"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_015056439.1"
                     /translation="MLYAIVISTILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(3924..5489)
                     /locus_tag="MWN28_RS26980"
                     /old_locus_tag="MWN28_26990"
     CDS             complement(3924..5489)
                     /locus_tag="MWN28_RS26980"
                     /old_locus_tag="MWN28_26990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_000447001.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEAEEKRLAALNEEARAVEVAQKNLAAAQSELAKVDEEIKTLNTRLSSSI
                     HARDAEMNTLSGKRNELAQASAKYKELDELVKKLSPRANDPLQSRPFFYATSRRTGAGK
                     IMEEKQKQVTASETRINQLNAEINGIQGAMSQANNNRNTAVLRVHESEENLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALESVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKTVDAGVSYIVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTIND
                     VLGI"
                     /name="colicin-like pore-forming protein"
     gene            5767..6186
                     /locus_tag="MWN28_RS26985"
                     /old_locus_tag="MWN28_26995"
     CDS             5767..6186
                     /locus_tag="MWN28_RS26985"
                     /old_locus_tag="MWN28_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312320.1"
                     /translation="MAWHTLQNALKMTGRSRSQFYRDMRAGLVSYRTGQDGRREFETSE
                     LIRAYGELKQDETPERHSAGQAENPHDQQTERILRELNELRQCLTLMLEDKQAQATERR
                     RQEAEREQLHKEIEQLRQALEQEKKRGFWARLFSR"
                     /name="hypothetical protein"
     gene            6270..6689
                     /locus_tag="MWN28_RS26990"
                     /old_locus_tag="MWN28_27000"
     CDS             6270..6689
                     /locus_tag="MWN28_RS26990"
                     /old_locus_tag="MWN28_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254598.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            7215..>7391
                     /locus_tag="MWN28_RS26995"
                     /old_locus_tag="MWN28_27005"
                     /pseudo=""
     CDS             7215..>7391
                     /locus_tag="MWN28_RS26995"
                     /old_locus_tag="MWN28_27005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     Typing          complement(2462..2543)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          2572..3118
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.841"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(259..1808)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.893"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atcagaagaa gcattaaacg cattctggcg tcgattacag aagaagcgga acgcgttcca
       61 ggctcctgtt gctatacaca ctacccacac taaccccatg gtttttgagc gccagaaacg
      121 cgtccgtatg cgttttaacg cgttcctgtg ttttcagcaa ggcctgactg aagctagttc
      181 ctagtgccct gaaccaacca ttatccaaaa aaatgcaccg gaaacgcgct gacaacactt
      241 tagcctgtct ttggttacca tcccagcgac gggccatcca gtgtccgttc ctgtatttcc
      301 agatggcgct cacgctctag gaatagctca tgttcacgtt cctgtatcag tagttcgtcc
      361 cttatctcct gctctcgctg tataactggc tcaagtgttc tgtctgctcg ctcaagtgct
      421 gcacctgctg actcaactgc atgacccgct cgttcagcat cgcgttgtcc cgttgcgtaa
      481 gcgaaaacat cttctgcaat tccacgaagg cgctctccca ttcgttcagc cgctgcatat
      541 agtcctgttg cagctgctct aaggcgttca gcaaatgtgt ttccagctct gtcactctgt
      601 gtcactcctt cagatgtacc cactctttcc cctgaaaggg aatcacctct gctgatttcc
      661 cgtacggaag gaccagaaat ttcctgttcc cgtcctgcac gaactccacg ccccatgtct
      721 tcgcgttcag tttctgcaat gtctcttcct gcttcctgat atcttccagg ttcgcctgta
      781 tcctcccgcc aagataccag agcgtcccgc cactcgcggt aaacaggaga aagactatcc
      841 ccagtaacat catgcccgta ttccctgcca gctttaacac gtccttcctg tgctgcctca
      901 tcgcctcttt caccccttcc cggtgtttct ccagcgattc ctctgtcgag gctgtgaaca
      961 gggctatagc gtctctgatt ttcgtctcgt ttgatgtcac agcctcgctt acagattcgc
     1021 cgagcctcct gaactcgttg ttcagcactt tctctgtaga ttcggctctc tctttcagct
     1081 ttttctcgaa ctccgcgccc gtctgtaaaa gattgctcat aaaacgctcc tttcagcctg
     1141 atattcttcc cgccgttcgg gtctgcaatg ctgatactgc ttcgcgtcac cctgacaacc
     1201 tcaactccca catctgtgag tgcctgaatc acatcctgac ggtcttttat ctctccggca
     1261 cggtaaagca catcaagccc ccgtgtaacg ctctcagcaa tcgcctgttt cgtttccggc
     1321 aggttatcag ggagtgtcag cgtcctgcgg ttctccgggg cattcgggtc atgcagcccg
     1381 taatggtgat tcaccagcgt ctgccaggca tcaattctcg gcctgtctgc gcggtcatag
     1441 tacggctgga ggcgttttcc gctctgtagc tccatgttcg gaatgacaaa attcagctca
     1501 agccgtccct tgtcctggtg ctcaacccac aggatgctgt actgattttt ttcgagaccg
     1561 ggcatcagta cacgctcaaa gctcgccatc actttttcac gtcctcccgg cggcagctcc
     1621 ttctccgcga acgacagaac acctgacgtg tatttcttcg caaatggcgt ggcatcgatg
     1681 agttcccgga cttcttccgg attaccctga agcaccgttg ccccttcccg gttacgctcc
     1741 cttcccagca ggtaatcaac cggaccactg ccaccgcctt ttcccctggc atgaaattta
     1801 actatcatcc cgcgctccct gttccctgac gacctgccgt aagctgcgca actctctctc
     1861 gatggccatc agtgcggcca ccacctgaac ccggtcaccg gaagaccact gcccgctgtt
     1921 caccttcctc gccgtctgat tcaggttatt cccgatggca gccagctgac gcagtaacgg
     1981 cggtgccagt gtcggcagct tcccggaacg ggcaaccggc tcacccaggc atacccgccg
     2041 catccatacc gccagttgtt taccctcaca gcgttcaagt aacctggcat gttcatcatc
     2101 agtaacccgt atcgtgagca tcctctcgcg tttcatcggt atcattaccc catgaacaga
     2161 aatccccctt acacggaggc atcagtgact aaacaggaaa aaacagccct taacatggcc
     2221 cgctttatca gaagccagac attaacgctg ctggaaaagc tcaacgaact ggacgcggat
     2281 gaacaggcag acatctgtga atcgcttcac gaccacgccg atgagcttta ccgcagctgc
     2341 ctcgcacgtt tcggggatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     2401 acagcttgtc tgtgagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcaggt
     2461 tttagggggt gtcggggcgc agccctgacc cagtcacgta gcgatagcgg agtgtatact
     2521 ggcttagtca tgcggcatcg gtgcggattg tatgaaaagt gcaccatgta cggtgtgaaa
     2581 tgccgcacag atgcgtaagg agaacatgca gatgccgatg ctcttccgct tcctcgctca
     2641 ctgactcgct gcgctcggtc gttcggctgt ggcgagcggt gtctgctcac tcaaaagcgg
     2701 tgatactgtt atccacacaa tcaggggata acgccggaaa gaacatgtga gcaaaaaacg
     2761 aagaccccag aaaaggccgc gccggaggcg ctttttccat aggctccgcc cccctgacga
     2821 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac ttaaagatac
     2881 caggcgtttc cccccggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2941 ggatacctct ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     3001 tggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     3061 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     3121 cacgacaaag cgccactggc agcagccgtt ggtaacttgg ttagcagagc gatgatggcg
     3181 caaatggtgc aacagagttc ttgaagtagt ggcccgacta cggctacact ggaaggacag
     3241 tatttggtat ctgcgctctg ctgaagccag ttacctcggt taagcagttc cccaactgac
     3301 ttaaccttcg atcaaaccgc ctccccaggc ggttttttcg tttacaggca gcagattacg
     3361 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctactgaaca gagatccccg
     3421 tcagtttaga agaagatgat ggagccacag tccctccctg aacatcacgt atatagttag
     3481 cctgacatcc aacaaggagg tttatagcta atattcccac aaaaaatctt ttcctcataa
     3541 ctcgatcctt ataaaatgaa aagaatatat aacgaggttt aatttatgag tttaagatac
     3601 tacataaaaa acattttgtt tggcctatat tgcacactta tatatatata ccttataaca
     3661 aaaaacaacg aagggtatta tttcctagcg tcagataaga tgctatacgc aatcgtgata
     3721 agcactattc tatgcccata ttcaaaatat gctattgaac acatattttt taagttcata
     3781 aagaaagatt ttttcagaaa aagaaaaaat ctaaataatg cccccgtggc aaaattaaac
     3841 ctatttatgc tatacaatct actttgtttg gtcctagcaa tcccatttgg attgctagga
     3901 ctgtttatat caataaagaa taattaaata cccaacacat catttatagt attaagttta
     3961 ttcttgtcaa taaaggcaca tagaatgcct gtaacaatag caatccccca gatacctaat
     4021 gtagttccag caagcacact aaaaagtaaa acaacaatat aactaactcc agcatctaca
     4081 gttttcttct ctaatgtaag aaatagtggc ttccagtcac ctgtatcctt aatttttagg
     4141 atatcagata ccacatcata tccaaaagaa acatgtcccg taatcttcaa gtacttagca
     4201 aactgatcta aatgcttagc ccagtcttca tacttaaccg attccagtgc attgaaaatc
     4261 gcatcacggt ctgctttgct gaatttctta tttaaaacat ccttgtattt ttcaaaagca
     4321 gctagagctt cattcacatt gctgattttc ttacctttag acttatcagc aagttcctgt
     4381 gccatttttg aatatttttc accatatttt tcagatagcg tttgataaaa gctaactgtt
     4441 gcatccacag catccttaat ctgcgagttc aggagattag tctgcgctgt tttcaaattt
     4501 tcttcagatt catgaacacg taaaacagct gtattacgat tattattagc ctgagacata
     4561 gccccctgaa ttccatttat ctcagcatta agttggttaa tacgcgtttc tgatgctgtt
     4621 acctgttttt gtttttcctc cataatctta ccggctcccg tccgtcgact ggtggcatag
     4681 aaaaaaggac gactctgaag cggatcatta gctcttggtg acaatttttt gaccagttca
     4741 tcaagttcct tatatttagc agatgcctga gccagctcat ttcgttttcc ggacagcgta
     4801 ttcatttctg catcacgggc atgaatactg gagcttaaac gggtattgag agtcttaatc
     4861 tcttcatcca ctttcgccag ctcagattgt gcagcagcaa gatttttttg tgccacctcc
     4921 actgcccggg cctcttcatt caatgctgcc agccgtttct cttcagcttc agccagtttc
     4981 aactggcgtt ctgtttcagc ctgctccttc tctatctctt tacgtcgttg ttctgcctcc
     5041 tgaaaagcct tttccgcagc ttccgcttct ttacgggctt tttcttctgc ttttgcaagg
     5101 cgcaaccgct ctgcttctgc ctgcatcgct gcattattgg catgagccag gtcaataacc
     5161 tccggatgag tggaattatg acgaagcgcc tcattcacaa tatccttcag atgttgagtc
     5221 agcgcatccc ggtttgcttt tgctttagcc tgtgcttctg cggcagcttt tgcccgggca
     5281 gcctgttctg cctgcgtttt cttcaattga gcagtagacc atttagctgt ggcatgaatg
     5341 gctgcagaac tttcactttt gctacctcca gttccaccac cgccgccaga gccactcccg
     5401 tctggattac cattcaaaag agtaatgatt acctgcccct tatcatcata aggaacacca
     5461 tctttatagt acgctacagc tgtttccatt ataaaatcct ctttgacatt aaaaacaaat
     5521 agttaaaagc aatatactgt atatataacc actggtttta tatacagctt aaaattacgc
     5581 cgctgcattt tccctgtcaa ccctgtggat tttcattttt gtgaaaacga tcaaaaaaac
     5641 agccacccac accacgtgct attcccaccc gatgccacaa aaaccagcac aaacattacc
     5701 gttctccggc cccagtatgt tttactgaaa ccatgatatg agacattaat gggacactgt
     5761 cacttcatgg catggcacac actccagaac gcactgaaaa tgacaggcag atcgcgttca
     5821 cagttttacc gtgatatgcg cgcaggcctt gtcagttatc gtacaggcca ggacggacgg
     5881 cgggagtttg aaaccagtga actgatccgg gcatacggcg aattaaagca ggatgagaca
     5941 ccagaaaggc acagtgcggg acaggcagaa aatccacatg atcagcagac agaacgcatt
     6001 ctccgggagc tgaatgagct gagacaatgc ctgacactga tgcttgagga caaacaggca
     6061 caggctacgg aacgccggcg ccaggaagcc gaacgggaac agctacataa agagatagag
     6121 cagctcaggc aggcactgga gcaggaaaaa aaacggggat tctgggccag actgttcagc
     6181 cggtgagcac cgactgcaag tgatgataag atagtcagcg gttaaatata agtcactgag
     6241 tacaaagatt taaaggtaag gagacactca tgagacgttc cggcggcaca ctgtatctta
     6301 tcgccctgct gacagcggca acggtcctga cagcctgcac gccaaagggc agtgtggaac
     6361 aacatacccg gcattacgtt tatgcatcag acgatggttt tgatcctaac ttttccaccc
     6421 agaaggccga cacaacacga atgatggtgc cttttttccg gcagttctgg gatatgggag
     6481 caaaggataa agcgacagga aaatcacgga gtgatgtgca gcaacgcatt cagcagtttc
     6541 acagccagga atttttaaac tcactccggg gcacaactca atttgcgggt actgattacc
     6601 gcagcaaaga ccttaccccg aaaaaatcca ggctgctggc tgagacgatt tctgcggttt
     6661 atctcgatgg ctacgaggga agacagtaag tggatttacc ataatccctt aattgtacgc
     6721 accgctgaaa tgcattcagc gcaatcacgg cagcagacag gtaaaaatcg caacaacccc
     6781 cccgaaaaac taccgcgatc gcgcctgata aattttaacc ttatgcatac ctatgcatcc
     6841 aggtgaatca caagcgaatt tccttactga gacgactgaa acggtttttt ttcacaattt
     6901 tcagctgttt tcgattccac aaaatagaag ttacaggata gggattaatc cccttcgccg
     6961 ccaaggggag caggatgagt caggggaatg tctattcacc tgcgctgtac aggctgccag
     7021 gtaaaagtat tcgctgcggc acgatactct tttgccctca tagcctttgc gcttcttact
     7081 tttcgttctt ccagaatcat tcttaagaca ttttcccgca cttttctcta acttcccgtg
     7141 gcaatttatc tctgccggac tgaaccgccc cggaaatcct ggagactaaa cttcctgaga
     7201 aagatgtaaa caggatgact aaaaatactc gtttttcccc cgaagtccgt caacgggcag
     7261 ttcgtatggt tctggaaagc cagggcgaat atgactcaca atgggcggca atttgttcca
     7321 ttgctccaaa gattggctgt acgccagaga ccctgcgtgt ctgggttcgc cagcatgagc
     7381 gggataccgg gtattacctt tcgaagaaat ttttgacatc ctctacgccc tgaaggaccg
     7441 ggttttacgg cgtaccagat aaaaaaaccc ctctactgaa gaggggtagt gtaaggaact
     7501 tgctgatttt cttgatatta ttaaccatca ataactgagc atacaaaaaa taacaaaact
     7561 ctgacacaga tacaaccatc cctctttgta taagaccgat gtgcatctca aaaaagaagc
     7621 gtgtttctgg aacacagaac aacaatgcac attgtagcca acaaattcac accagaaaca
     7681 cgctctgttt tttaatttac acaagaatat atgtctggaa aatcaggaag aagaaattaa
     7741 tgggcattct gtatacgaag ggagagcgcg tttccggtgc cccatattag atattatccc
     7801 tgcaacaaac accagagacg cgctcataat tcttctagcg taaatgatcc tttcaggcaa
     7861 gaaaaaagcc gtgattctgt atcacggcct taattttatc gaaaacacgg attttagtat
     7921 gcacatgact gtactcctgt ggagtacaag gaaatcataa tcagcgacca aaccatttat
     7981 caacagaaaa ccaacatatt gatgttattt ttgtgacaaa tgttacaaat tcgaaaccac
     8041 tgtattttta ctgcaacaaa atctggttga tctacaagtt acactggggt aaaaagttgt
     8101 ccggaatgtt tctgcgtagt aaagcctcta gccggattgc ctttgcaaaa acgcaggaac
     8161 gttcccccct tcatcagtac tcctgacgct gtaaggagct gactgatgca gacagggctg
     8221 atgatatcta ataaaagcta tctcattttt gcgcgtacat aaaataaatc aggcacttcc
     8281 ctacactccc cctccggaat tgagggggtt ttctttacaa tgtaaataaa ttgcttgcgt
     8341 aaagttcaaa aaaactgcaa atttttactg tagtgtcgag cttcatgtag ctcaccaggg
     8401 catggactaa aaagaagtaa acccatcttt taccccccct tatacggggg gttctttata
     8461 gcacaaagaa cacttataat ttatcatctc acagctataa atataaaaga ataatattct
     8521 aaactacagt tatttatgca gaacataaca tcaaactcac aataaatata aaacaattat
     8581 tttataactg ttcacttatt atattcagac atatcagaaa cacgactcaa tttctttttt
     8641 tatttaacaa aatccataaa aaacttatgg ttgaaatcac atttaaactg tgaaggatat
     8701 catccggaga aatttttcaa tccgacactc catagaatgg acctttaaga ctttatcctc
     8761 atcatgtatg aggatttttt tctggcaaac agtatctttc agtaatcctt gctcagttcc
     8821 gattctgacg tatccccgtg tggatctgaa aaggagataa ttacgctctg tggccaacag
     8881 gaataactca agcagctcct ctctgatacc
//
