LOCUS       NZ_CP095278             2496 bp    DNA     circular CON 03-APR-2024
DEFINITION  Enterobacter hormaechei subsp. xiangfangensis strain HD8642 plasmid
            pHD8642-2, complete sequence.
ACCESSION   NZ_CP095278
VERSION     NZ_CP095278.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27409789
            Assembly: GCF_026109755.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei subsp. xiangfangensis (Enterobacter
            xiangfangensis)
  ORGANISM  Enterobacter hormaechei subsp. xiangfangensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Du,H. and Zhu,Z.
  TITLE     Complete sequences of chromosome and plasmids of HD8642
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Du,H. and Zhu,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2022) Department of Clinical Laboratory, The
            Second Affiliated Hospital of Soochow University, Xihuan, Suzhou
            215004, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_026109755.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 06:52:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,852
            CDSs (total)                      :: 4,738
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 15 of 48
            Pseudo Genes (incomplete)         :: 29 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            Pseudo Genes (short protein)      :: 1 of 48
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095278.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Enterobacter hormaechei subsp. xiangfangensis"
                     /mol_type="genomic DNA"
                     /strain="HD8642"
                     /isolation_source="respiratory tract"
                     /host="Homo sapiens"
                     /sub_species="xiangfangensis"
                     /db_xref="taxon:1296536"
                     /plasmid="pHD8642-2"
                     /geo_loc_name="China: Sichuan, Chengdu"
                     /lat_lon="30.67 N 104.05 E"
                     /collection_date="2020"
                     /collected_by="Sichuan Provincial People's Hospital"
     gene            complement(join(2323..2496,1..54))
                     /locus_tag="MU851_RS23845"
                     /old_locus_tag="MU851_23810"
     CDS             complement(join(2323..2496,1..54))
                     /locus_tag="MU851_RS23845"
                     /old_locus_tag="MU851_23810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1256..1474
                     /locus_tag="MU851_RS23850"
                     /old_locus_tag="MU851_23815"
     CDS             1256..1474
                     /locus_tag="MU851_RS23850"
                     /old_locus_tag="MU851_23815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182407767.1"
                     /translation="MFGFSSGALMTFNDDERHLLVSVVSGWLRRAEGDAGAMMLDAYRQ
                     ILSETEPAARAVMLEFLESVRIHYVSS"
                     /name="hypothetical protein"
     gene            complement(1772..1957)
                     /locus_tag="MU851_RS23855"
                     /old_locus_tag="MU851_23820"
     CDS             complement(1772..1957)
                     /locus_tag="MU851_RS23855"
                     /old_locus_tag="MU851_23820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032673404.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEKDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          977..1146
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.698"
                     /coverage="98.0"
                     /evalue="2.0600000000000002e-57"
     Typing          1638..1725
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.382"
                     /coverage="100.0"
                     /evalue="1.5000000000000001e-30"
     Typing          complement(470..1052)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.331"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 gagggctgaa agcgcaactg ccgctgcgaa cactgaaaca aaaatctttt tcaaagccag
       61 ctccttttat ccatatcagt tttggtcact aaccatctaa ctataggtta ttttttatgt
      121 gaaaagaggc ttgatggtgg gctgtatatc gagttaggcg cgaggtgcta tggtcaaagt
      181 gtggtgtcaa gcggggagga ggcttatatg agatgtaatc cagcagatac atataccaag
      241 atgatttaag tattcctctt tgtgaggttg gatgtctaat ttataaagga tcttcttgag
      301 atcctttttt ttcgcgcgta atctcttgcc ctgtaaacga aaaaaccacc ctggcaggtg
      361 gtttttcgaa ggttaggtaa tcctggcaga tcccctaacc gtggtaacag tcttgtgcga
      421 gacatgtcac caaatttgtc ctttcagtgt agcctcacta aggccgccac ttcaagaact
      481 cttgatacat ctctcgcaca tcctgtttac caatggccgt tgccaatggc gattagtcgt
      541 gtctttccgg gttggactca agttgatagt taccggataa ggcgcagcgg tcggactgaa
      601 cggggggttc gtgcatacag tccatcctgg agcgaactgc cttcccggaa ctgagtgtca
      661 ggcgtggaat gagaaaccgc ggccataaca gcggagtgac accggtaaac cgaaaggcag
      721 gaatgcgggg gagcacgagg gagccaccag ggggaaacgc ctggtatctt taagccgcat
      781 cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag
      841 cctatggaaa aacggctttg ccgtggcctt atgccttttt cgctaagaac cttcctggca
      901 ttctccagga aatttctgct ctgtacgtaa gcccgtaccg ctcgccgcag ccgaacgacc
      961 gagcgcagcg agtcagtgag cgaggaagcg gaatatctcc tgtatcacat attctgctga
     1021 cgcaccgatg ccgccttttt ctcctgccac atgaagcact taactgacat ccgcatccgt
     1081 gccaacatat taagccagta tacactccgc tagcgctgcg tgactggtcc ggcgctgcgc
     1141 ccggaacccg cctgtaccgg ttaagcagcc gttctggtct gactgcgatt tttttcattc
     1201 ctgcccgcta ccctgtaaac ctttcttctg cgttgccgtt aacaggaggt aagccatgtt
     1261 tggtttttcc tccggagccc tgatgacttt taatgatgat gagcgacacc tgcttgtgtc
     1321 cgttgtttcg ggctggctcc ggcgtgccga aggggatgcc ggagccatga tgctggatgc
     1381 ataccggcag attttgtctg aaaccgaacc ggccgcaagg gcggtcatgc tcgaatttct
     1441 tgaatcagtt cgcattcatt acgtttcgtc ctgagcattt cctgaagcat ttcctgaatt
     1501 tttgtccata ctcaatccag gcggccgtca cggacggtgg tcgtcgttcc atgatgctgt
     1561 tatggaagat tgagagtaat ctaacttgat tgagttccgg gcgtgtcgcc cggttttttt
     1621 tgctgtttcc cggtgtccgc tcccggacag gatgtgcatt tgtaataccg cgcacgcggc
     1681 ggcatcacaa ataccccgtt cagggctgcg ccccgacacc ccgccatcgc ctcacggccg
     1741 cgcactgaat tgcgcacccg ttcaccgatt ttcacgcatc tttctcgcct atctgtgcgt
     1801 tcagcatggc gtaaagcgct tcagcctgat cgtgcagatg ctcacagcag ccggctgcct
     1861 catccagatc catctgttcg agccgttcga ggaggaggag tgtctgtgag cggataaatt
     1921 tggccatgtt gagggcggcg gattcctgtc tggtcactgg tgattttccc cgtctggtga
     1981 tgatttttgc gtgaaaagag catgccgtca ttctggtgca tagtcatgca tgccgttaaa
     2041 atttaacagg cgcgtttctg acgggttccg gggtggtttg ttgtggtttt tgtcatggtt
     2101 ccgtcagaaa cgcgctgagc gcgtctgagg cggtgcgggc aacggatgtt atggtaaatt
     2161 cttgggggct tgattataaa ataccgcact tcctcttatg tcttgtgctt tttagacaac
     2221 cctgaaagct tttagctttg gtgatgcaat ttgctgggat tttggcggag aaccacaggt
     2281 aaagaaaaag gccacattag cggccttttt cggagaatat gctcagtgcg gaatttcgga
     2341 gacttcttta acgtcggttt tattgatctg ctgtttgaca ccattcgcgt ctgtatagcc
     2401 aagcaggcct gagtctgatt ctttaggttt gccgtcgctg acaatagtgc gaccgtcatt
     2461 ggtgtgtatg gcatagcttg tgcgcgtgca gccggt
//
