LOCUS       NZ_CP095268             5596 bp    DNA     circular CON 05-JUN-2024
DEFINITION  Klebsiella pneumoniae strain 21091025 plasmid p21091025_4, complete
            sequence.
ACCESSION   NZ_CP095268
VERSION     NZ_CP095268.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27363257
            Assembly: GCF_022982575.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   He,Z.
  TITLE     Comparative Genomic Analyses of Klebsiella pneumoniae in Anhui,
            China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   He,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2022) School of life science, University of
            Science and Technology of China, 1129 Huizhou Ave, Hefei, Anhui
            23005, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.4.9b; SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022982575.1-RS_2024_06_05
            Annotation Date                   :: 06/05/2024 03:14:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,609
            Genes (coding)                    :: 5,470
            CDSs (with protein)               :: 5,470
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 51 of 139
            Pseudo Genes (incomplete)         :: 90 of 139
            Pseudo Genes (internal stop)      :: 14 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095268.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="21091025"
                     /isolation_source="sputum"
                     /host="Homo"
                     /db_xref="taxon:573"
                     /plasmid="p21091025_4"
                     /geo_loc_name="China: Anhui"
                     /lat_lon="31.85777 N 117.291453 E"
                     /collection_date="2021-09"
                     /collected_by="Zhien He"
     gene            369..527
                     /locus_tag="MU321_RS28690"
     CDS             369..527
                     /locus_tag="MU321_RS28690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            885..1211
                     /locus_tag="MU321_RS28510"
                     /old_locus_tag="MU321_28510"
     CDS             885..1211
                     /locus_tag="MU321_RS28510"
                     /old_locus_tag="MU321_28510"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            1237..1782
                     /locus_tag="MU321_RS28515"
                     /old_locus_tag="MU321_28515"
     CDS             1237..1782
                     /locus_tag="MU321_RS28515"
                     /old_locus_tag="MU321_28515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            1848..2198
                     /locus_tag="MU321_RS28520"
                     /old_locus_tag="MU321_28520"
     CDS             1848..2198
                     /locus_tag="MU321_RS28520"
                     /old_locus_tag="MU321_28520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2268..2534)
                     /locus_tag="MU321_RS28525"
                     /old_locus_tag="MU321_28525"
     CDS             complement(2268..2534)
                     /locus_tag="MU321_RS28525"
                     /old_locus_tag="MU321_28525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2518..2742)
                     /locus_tag="MU321_RS28530"
                     /old_locus_tag="MU321_28530"
     CDS             complement(2518..2742)
                     /locus_tag="MU321_RS28530"
                     /old_locus_tag="MU321_28530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(3409..3621)
                     /locus_tag="MU321_RS28535"
                     /old_locus_tag="MU321_28535"
     CDS             complement(3409..3621)
                     /locus_tag="MU321_RS28535"
                     /old_locus_tag="MU321_28535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3628..4137)
                     /locus_tag="MU321_RS28540"
                     /old_locus_tag="MU321_28540"
     CDS             complement(3628..4137)
                     /locus_tag="MU321_RS28540"
                     /old_locus_tag="MU321_28540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4172..4876)
                     /locus_tag="MU321_RS28695"
                     /pseudo=""
     CDS             complement(<4172..4876)
                     /locus_tag="MU321_RS28695"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4815..5159)
                     /locus_tag="MU321_RS28550"
                     /old_locus_tag="MU321_28550"
     CDS             complement(4815..5159)
                     /locus_tag="MU321_RS28550"
                     /old_locus_tag="MU321_28550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5149..5325)
                     /locus_tag="MU321_RS28555"
                     /old_locus_tag="MU321_28555"
     CDS             complement(5149..5325)
                     /locus_tag="MU321_RS28555"
                     /old_locus_tag="MU321_28555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     Typing          complement(5412..5488)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          81..478
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(3294..4825)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gctgcgctcc tgccgttcgg ctgcggcggg cgtgaccgac tcacggataa cgggataaag
       61 caaaggcgct caaatcagaa agggccggag aaaaggatgc ggcgttgccg tttttccata
      121 ggctccgcct cccctgacga gcatcacaga aatggacgct caagtcagag gaggacgaaa
      181 cccgacagga cttaaagatg ccggtgattt ctctccggcg gctccctcgt gcgctctcct
      241 gttccgaccc tgccgtttac cggatacctg ttccgccttt ttccctttcg ggaagcgtgg
      301 cgctttctca tagctcacgc tgctggtatc tcggctcggt ttaggtcgtt cgctccaagc
      361 tgggctgtat gcacgaaccc cccgttcagc ccgactgctg cgccctttcc ggtaactatc
      421 atcttgagtc caacccggac ggacacgcaa aaacgccact ggcagcagcc attggtaact
      481 ggatatatac gagagagata ttgagaggtc ttgaagtggg tgcctaactg cggctacact
      541 gaaagaacag ttttggtatc tgcggtctcg tatacccagt tacccggtta agcagttccc
      601 caactgactt aaccttcgat caaaccgcct ccccaggcgg ttttttcgtt tacagtgcaa
      661 gagattacgc gcagaaaaaa aggatctcaa gaagatcctt taaatgatcg tcagttctgt
      721 tatccccgga gctccatcca tcccacggaa aaacacccgc cactgatgcc ctcagcgcac
      781 tgcacatcat gtacaaacca ctccctccct ccttttttat tgcgtttctt aatgcgcaat
      841 ggtagctttg tgcgcaacaa aaaacgcaat agcaaggggg ggtaatgaga gaaagaacaa
      901 atatcatgct agatatagga ttaaagcaat ccttacaacg gctttcgaat aaaaccaaaa
      961 tgagtctaag tgatcttatt aaccagagat tatcaggttc actttcagtt gaagaacgca
     1021 ttggcgaaaa gcctgaatgg atgagaaacc ttgatcccta tctcgccaat cacttttcaa
     1081 atattgagtt accgctccct tcaccattaa aagaatacca agaaaaaatg gatgagatag
     1141 attcaattat taatgacatg atggttctaa aggaatattt cagagctaag ttttacgacg
     1201 aaataaaata ggcacgctat acggaggcgg agaattatgt tttttaaacg gtatttaacg
     1261 gagaaggaaa tatacaaggc agcactccca attgttaggc ttcaagggag agttaacact
     1321 tcattacgtt ttttatccaa atactcagat gtaaacaaaa atggattaat tgcattgcat
     1381 cttaatttat tacattttat ctcatacaac atacacggtg tagagtctct gaaaatcact
     1441 caagcaggca taaacaaagc tgtacaacaa agccctcacg cagatgaaat aaaaaatatt
     1501 gcgcaggcaa taggacgcaa aggacctgag tacatcgaag gattaattag aaacccatgg
     1561 gggaagaaaa atgataatcc actaatattt ataatgagca ttgaagaaag tataaaagaa
     1621 acccttaact caggttatat gttatataga gtagctccca taactgaggg tgaagcagct
     1681 gctttcatgg atgttcttaa acaattatta ttatttgagg ataattttcg cctttttggt
     1741 agagacttgg tttataaaat agaaaaatta aaaatcagat aacaaagtat ggtgaacgaa
     1801 aaatgaaaga gaggatagtg aaatgtgtaa taccaagatt ctcaactatg tattggttaa
     1861 ttgcgttcct agcatttata ttccccggtc gatcttttgc tgacggttac accgcttact
     1921 accgatgtaa tggtactcag gaagcatctc ttactgttaa agataacaaa ctaagtatgg
     1981 ttgtaggtct ttctaagttt aataggcaac tggatactta tagattcaaa gaaaagggaa
     2041 tcgaagtagt aatgcagaga gctgcttctg atgataactc tgaggccata atgtctttta
     2101 ttatgcctga cgttaacaat atgaccttat tagttaaaaa taacggctac attatcgtta
     2161 agacaacctg ctggttaact aaaaccgaca aacagtagaa cacgtagtta gtcgagtatt
     2221 taccataata cctgttgtcg gcatcaaaaa agcccgcata gcgggcttca gtcgtaaact
     2281 tcgcggcgat gtccaatcgt ggcggcaagg ataacaagct cgtcatctcg gatctcacac
     2341 agaacgcgat aatctcccac ccgatagcgc cagaactcgc ccagctcacc tttgagcggc
     2401 ttccctgact ggcgaggatc ggcagcaact gcaatgcgta aactcataaa atccacaatc
     2461 cgtcgtgcgt tctgtttgtc catcttgcgt aacgatttga gcgcccgatc ggaatagtta
     2521 atcgtccaga ccaagtgact tcctcacgtc ttcagacgaa tgcactgctt catcaccacg
     2581 gcgaacgcgt gcagcagttt ctgctgaaag ataataatcc tccaggtctt ccagatgcgc
     2641 cagtattgct tcccgcgcat aaaacgtctt tgttctgccg gtttgcttcg ccagcgagtc
     2701 cagacgggac tcaatctcat cagataaccg gatagcaagc atataaacct cccgaacacc
     2761 atttgctata catgtatatc aacagtacca caatcaacct aaaagaacaa aaaataatct
     2821 tgttagcccc tcttgataat ccgtaacgcg gtcaatatat ctagcttgag ggctttcctc
     2881 ttaactattg aaaggataac gattatgggt cttaagccgg gtccgaaacg aattgccgat
     2941 tcaacagggg aacctgataa acgccagcgt gacaataaaa aaacgccggg aaatactgac
     3001 aaattgaaac ccagcaaatc ttcaaaaaaa taagtcagta gttaaaagcc ctccggggct
     3061 tttttgtatc tgccgttcac gctgattaaa cataacaccc gttgcccgca ccgccccgga
     3121 cgggttcgcc gcgaacgggg tggcagtcag gtaaaaccgg caacaaacca cccgataaac
     3181 cgccccgttc gcgacagata aattttacct ggctgcatag ccatgcagcc aggtgaatga
     3241 catccggttt cagcgtaaat gcccgatgag aaccctgttt tcctgcattt cagcccagcg
     3301 acggaccgtc atagcttcgc tgccgggaac gcgcttccgt cagttccttt tcgtgctgcc
     3361 gttgctcgtg cgcccgttct gcggccacgg ccatctcatg acgctgcact accggctcaa
     3421 acgctgcagt tgcccgctca agcgttccac ctgctgactc aagcgcgtca cgcgctcgct
     3481 cagcgccgcg ttctcccgtt gcataagccc tgacattttc cgccattccc cgaaggcgct
     3541 ctcccactcg tccagccttt tcgagtagtc ctgctgtagc tgctctaatg cgctcagcaa
     3601 ctgtttttcc agctcggtca tcggcactta ctccttcacc agtctcagcg cgttctgctt
     3661 cgaccgcccg ttcccgaccg tccagctctg cgtcctgtct acggccgtcc ccttcggcag
     3721 caccagaaaa cgtccgttgc tgtcttccag gtacgtcacg ccccaggtct tcgcgttcag
     3781 cttcgacagc gctgcgttct gccggtcgat ttcggccaga tttgacgcta tcaagctccc
     3841 ctgataccac aacaccccgc tcaggctggc gaacagcagt cctgacacca tcgtgatggt
     3901 cagccatgtc cgcccgacca tacgcatcac gctcaggcgg ttcgactgca tggccgcgct
     3961 cataccctgc tcgtgggcgc ggatggcggc gctgattctc ttcccgcttt cgctcagttc
     4021 cgctctgaca gacttctcgt gctcgctgaa tgcggctttc agcatttcgc cggtactctg
     4081 ctgctgcgct ttcgatttct gctctaagtc cttcgccagc gttaaaagac tgttcataga
     4141 tggctccctt aagtcggatg tttcgccctc cgtccgggtc ggcaatgctg atgctgctct
     4201 ttgtggtgcg cacgacctca aaacctgcgc tttccagcgc ctcagtgacg tcctgacgcg
     4261 ttttaagctc ccctgacgag gcgagggcca gtaagccccg cgtaatagcc tctgcggctt
     4321 cctgcttcgt tttgggtaac gaagatggcg tcaccagcgc gcgccggttc tccggtgcgt
     4381 tcgggtcgtg cagccccagt cgcccgttca ccacggtctg ccacgcatcg atgcgcggac
     4441 ggtctgcccg gtcgtagtac ggctggagac gcctgccggt cagcagttcc gtgttcggta
     4501 ccagaaaatt cagttccagc cgccccttgt cccggtgttc aacccacagc acgctgtact
     4561 ggtctttatc gagtccgggc atcagaaccc gctcgaagct cgccatcagc ttttcacgct
     4621 ggccgggcgg taaatcctgt tcagcaaaag acaggacgcc ggaggtgtac tttttggcgt
     4681 agggcgaggc gtcgataagc tcccggacct cctccggctt tccctgcagg acgatggccc
     4741 cgtcgcgctg gcggtctttc cccagcagat aatccaccgg cccggcaccg ccgcctcgcc
     4801 ctcgcggatg aaacttaacg atcatcgtct gccccctttt ccagcacggc atgccgcagc
     4861 cgctcgagtt cggcatcaat ggccatcagc gcggccacca cctgaacccg gtcagcgcct
     4921 gaccactggc cgccgttaat tttccgggcg atctggttga ggttgttccc catcccggca
     4981 agctggcgca gcaaaaccgg atcgatggac ggcagccgca acgaccgcgc cgggcgcgta
     5041 tcgagacagg tttcccgcat ccacaccgcc agttgccttc cattgcagcg ctccagcagc
     5101 tgctggtact ccccatcggt cacccggata cctaaaaaac gggtacgctt aaccgccatc
     5161 ctctcctccg atatccgcaa aatgtgaccg ggctgaacgc tcaagctctg ccatctgctc
     5221 ccggcggtaa tcttcctcct gtgcctgacg catcagattt gccatcactg ccggatgcca
     5281 ggatcttttt tttacgacga gcggcttctt cttcggcttc tgcattatcc ctccttcgca
     5341 aacgccggac ggcacggaaa agttttccgg gtcgctccgg cgttggacaa gcgccagcgc
     5401 gtcagtggtg ttagcgggtt tcggggcgca gccctgaacc agtcacagcg tttacgctgt
     5461 gtatactggc ttactatgtt ggcatgagag cacgattcat gaaagtgcct catgtggcag
     5521 gtgtgatatg tggctcagga gcgtccgcaa taccatgcac ggacagatga aatgacgcct
     5581 cctcgctcac tcggta
//
