LOCUS       NZ_CP095263             5535 bp    DNA     circular CON 05-JUN-2024
DEFINITION  Klebsiella pneumoniae strain 21080937 plasmid p21080937_10, complete
            sequence.
ACCESSION   NZ_CP095263
VERSION     NZ_CP095263.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27363256
            Assembly: GCF_022982615.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   He,Z.
  TITLE     Comparative Genomic Analyses of Klebsiella pneumoniae in Anhui,
            China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   He,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2022) School of life science, University of
            Science and Technology of China, 1129 Huizhou Ave, Hefei, Anhui
            23005, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.4.9b; SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022982615.1-RS_2024_06_05
            Annotation Date                   :: 06/05/2024 03:31:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,887
            CDSs (total)                      :: 5,766
            Genes (coding)                    :: 5,588
            CDSs (with protein)               :: 5,588
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 72 of 178
            Pseudo Genes (incomplete)         :: 118 of 178
            Pseudo Genes (internal stop)      :: 24 of 178
            Pseudo Genes (multiple problems)  :: 35 of 178
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095263.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="21080937"
                     /isolation_source="sputum"
                     /host="Homo"
                     /db_xref="taxon:573"
                     /plasmid="p21080937_10"
                     /geo_loc_name="China: Anhui"
                     /lat_lon="31.85777 N 117.291452 E"
                     /collection_date="2021-09"
                     /collected_by="Zhien He"
     gene            complement(127..984)
                     /gene="repA"
                     /locus_tag="MU320_RS29265"
                     /old_locus_tag="MU320_29265"
     CDS             complement(127..984)
                     /gene="repA"
                     /locus_tag="MU320_RS29265"
                     /old_locus_tag="MU320_29265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator RepA"
                     /protein_id="WP_032495102.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSRF
                     DFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECGLA
                     TESDAGVLSITRATRALTSLAELGLITYQTEYDPLIGCNIPTDITFTPALFAALDVSEV
                     AVASARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIKRARA
                     RRDADRERQDIITLVKRQLTREIAEGRFTASREAVKREVERRVKERMILSRNRNYSRLA
                     TASP"
                     /name="repA"
     gene            complement(977..1051)
                     /gene="tap"
                     /locus_tag="MU320_RS29395"
     CDS             complement(977..1051)
                     /gene="tap"
                     /locus_tag="MU320_RS29395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375168.1"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]; GO:0031556 - transcriptional attenuation by ribosome
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="WP_001375168.1"
                     /translation="MPGKVQDFFLCSLLLCNISAGWCD"
                     /name="tap"
     gene            complement(1296..1544)
                     /locus_tag="MU320_RS29280"
                     /old_locus_tag="MU320_29280"
     CDS             complement(1296..1544)
                     /locus_tag="MU320_RS29280"
                     /old_locus_tag="MU320_29280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="WP_000083837.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKELR
                     VFIPAALKAQLQEMCDAEGVTQAEMIAELIKQKSAFS"
                     /name="replication regulatory protein RepA"
     gene            complement(1828..1977)
                     /locus_tag="MU320_RS29285"
                     /old_locus_tag="MU320_29285"
     CDS             complement(1828..1977)
                     /locus_tag="MU320_RS29285"
                     /old_locus_tag="MU320_29285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="WP_001312851.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAY
                     EARK"
                     /name="Hok/Gef family protein"
     gene            complement(2282..2614)
                     /locus_tag="MU320_RS29290"
                     /old_locus_tag="MU320_29290"
     CDS             complement(2282..2614)
                     /locus_tag="MU320_RS29290"
                     /old_locus_tag="MU320_29290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_152916585.1"
                     /translation="MAFISMTRQKAPYRYASKTRLSIRRTDSGSAGCYVTVAVRGKQEF
                     IIAELDSEIRKIRLKLTDSYEEGVRLSSGTFTLPARFCREILPDDVRSITIILEKSDDE
                     WWYGSY"
                     /name="hypothetical protein"
     gene            complement(2676..3275)
                     /locus_tag="MU320_RS29295"
                     /old_locus_tag="MU320_29295"
     CDS             complement(2676..3275)
                     /locus_tag="MU320_RS29295"
                     /old_locus_tag="MU320_29295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="WP_032083981.1"
                     /translation="MATGKSEKQQVIAFIDYENCSNLTEISLNIYTELIIFAGAKQNNV
                     ALPVNAFPEAVKITLRHVSEVSRNNVDFHLVLELGQCVCHYGPEKEYHIVSADKGYDGI
                     VRTLQARGIRCRRVSPDKASSVKMAAPDMDIVIRWANKIQQAAREVRNMPVTEKTFNNY
                     LKSQMRGEWRDVLTRGIRDELVRRGVMKIQGNKIIW"
                     /name="PIN domain-containing protein"
     gene            complement(3661..3861)
                     /locus_tag="MU320_RS29300"
                     /old_locus_tag="MU320_29300"
     CDS             complement(3661..3861)
                     /locus_tag="MU320_RS29300"
                     /old_locus_tag="MU320_29300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015059022.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVSLEVRGFRKEAAEAWLLAARV
                     APRTDWQQFARQRAERCQRRR"
                     /name="hypothetical protein"
     gene            complement(3993..4553)
                     /locus_tag="MU320_RS29305"
                     /old_locus_tag="MU320_29305"
     CDS             complement(3993..4553)
                     /locus_tag="MU320_RS29305"
                     /old_locus_tag="MU320_29305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="WP_000139328.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLAE
                     KAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRDVL
                     LEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEAYAA
                     ARLDKIRRQNRIKAELQAVLDEK"
                     /name="fertility inhibition protein FinO"
     gene            complement(4608..5354)
                     /gene="traX"
                     /locus_tag="MU320_RS29310"
                     /old_locus_tag="MU320_29310"
     CDS             complement(4608..5354)
                     /gene="traX"
                     /locus_tag="MU320_RS29310"
                     /old_locus_tag="MU320_29310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="WP_000205770.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLMV
                     LDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQFAY
                     YLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAGLLM
                     LAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSCAGKS
                     LPRFWPGDFFPVFYACHLAVLGVLAL"
                     /name="traX"
     gene            complement(5374..>5535)
                     /locus_tag="MU320_RS29315"
                     /old_locus_tag="MU320_29315"
                     /pseudo=""
     CDS             complement(5374..>5535)
                     /locus_tag="MU320_RS29315"
                     /old_locus_tag="MU320_29315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein traI"
                     /name="protein traI"
     Typing          complement(128..882)
                     /name="IncFIA"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          983..1241
                     /name="IncFII"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.615"
                     /coverage="100.0"
                     /evalue="1.05e-131"
ORIGIN
        1 ccggatgctc cagaaggtgg atagtgaggt tgttttttgt atgtggtgct gattttttgt
       61 tcgctggcag gcttcaggcg tgcgaatgcc tccggttcgg gccggattat tcagaagagg
      121 tcactttcag ggggaagctg tggccagccg gctgtaattg cggttacgtg acagaatcat
      181 gcgctccttc acacgacgct ccacttcacg ttttaccgcc tcacgactgg cagtgaagcg
      241 cccttccgcg atttcgcgcg tcagctgccg tttcaccagg gtgataatat cctgacgttc
      301 cctgtccgca tcacgacgcg cacgggcacg ctttattccc cgggacttaa gctctgtctg
      361 gtaactgcgg aaacgctcac gaacaaaacg ccaggctttc gctatcagtt catccatgcc
      421 cagggtatcc agcccctgct ttttgcgttg tctgttttcc cattccacac ggctgcggcg
      481 tgcggaagcc accgccacct cagacacatc gagagcggca aacagcgccg gtgtgaacgt
      541 gatatcggtc ggaatgttgc aaccaataag cgggtcatat tccgtctggt aggtaatcag
      601 acccagctca gccagggacg tcagggcacg ggtggcacgg gtgatggaga gtactccggc
      661 atcagactcc gtcgccagtc cacactcaat ggccagcgtg gtgatggagc actggacacg
      721 gttggccagc ggatcatagt gaaaacagag cccctgcagc agcgcatcaa tagcccgacg
      781 acgcagtacc ggtggcatgc gccgacgcag accacgcgaa cgggcatgtg ccacatgaat
      841 ggcgaaatca aaacgggagg tgaagcctac cgccttttcc atcagttttt cgcaaaactt
      901 cagcgttccg gcaccttcac ggggtgtaaa caccggattc gggttcttta cctggcggta
      961 ataagtttgt tgaagatcag tcacaccatc ctgcacttat gttgcacaga aggagtgagc
     1021 acagaaagaa gtcttgaact tttccgggca tataactata ctccccgcat agagcaacag
     1081 cttctatgca gtttcttgtt agccccggta atctttctga ctcgacaaag ttaaagaaga
     1141 ttatcggggt tttcgctttt ctggctcctg tacatccaca tcagaaccag ttccctgcca
     1201 ccttatggcg tggccagcca caaaattcct taaacgatca gcaatctatc acgcacacca
     1261 caaacaggca agaatgtgat gtcgctacat tttagttagc tgaaagcact cttctgcttt
     1321 atcagttcag caatcatctc agcctgagta acaccttcag cgtcacacat ctcctgtaat
     1381 tgcgctttta acgcagcagg aatgaacact ctgagttctt tatgtgtggc acgttttctt
     1441 gccagagatg cctgctgacg ttctgcaagc gtcagaggat taccttttcg gtatgctcgc
     1501 tttgtacctg atgaggaagt cactgcattt tctgtctgcg acatctcgcc tcctcaattc
     1561 tcaaacaggg atcgtttcgc agaggatact acagtttttt gaaatcagcg acttgaaagt
     1621 tgtgatgatg atctgtgttg gcgcacaaat catgtctgat tacgaccgtt taatgtgatt
     1681 ttgatgatga aatgactgaa aaacaggaaa tgccttaacc tggatgccgg ccccgggcgg
     1741 gaccggctgt ttctggcatg tcgggtgtct gcccctgaaa tacctcacac ttccggaaag
     1801 cggggacttc cgtccccgcc cggcagctta cttccgtgct tcgtaggcca gagttacctg
     1861 caccactgta tttcccctgt gaatattcag ttcacataac cgttccctga atatcagtga
     1921 aaaacacaat accgtggcac acacggcgag caacccgata agggcatatt tcgtcataaa
     1981 ctttgacctc tctgcaaaga agaggctaca atcagtagtg ctaagtactt catgcagacc
     2041 tcacgagtta atggattaac gagtggggtc ttcgcatttc tggtacctgc gtcttgcctg
     2101 aaccagaaag gctcagacac ccgccgcgat aataccacat tcctgtctgg ttacgacgac
     2161 ctccatgccc ctgcttttta ttgcagaaac gccgggagcg gggaaaactt gctttttccc
     2221 gtttccgggg ttggacaact gagcaacgcg aaggcgtcag tgtccggagt tctgccacat
     2281 atcagtaact cccgtaccac cactcatcgt cgcttttttc cagaataatg gtgatactcc
     2341 tgacgtcgtc cggcagtatt tcccggcaga atctggccgg aagcgtaaac gtgcccgaag
     2401 acagccttac cccttcttca taggagtccg tcagtttcag cctgattttc cggatttctg
     2461 aatcaagttc agcaataata aattcctgct tcccccgaac ggcaaccgtc acataacacc
     2521 cggcagagcc tgagtctgtt cgtcttatcg ataaccgggt cttgcttgca tatctgtacg
     2581 gcgctttctg tcttgtcatg gaaataaatg ccattattga ttttcgacct ttctgttttt
     2641 ataaataaaa taaatatcat tttattctgg cagtcttacc agatgatttt attcccctga
     2701 attttcatta ccccgcgacg gaccagttca tcccggatac ctctggtcag gacatcacgc
     2761 cattcaccgc gcatctgtga ttttaaataa ttattaaatg tcttttctgt taccggcata
     2821 ttacgaacct cccgcgctgc ctgttgaatt ttgtttgccc accggataac gatatccatg
     2881 tcgggtgcag ccatctttac tgatgacgcc ttatccgggg agacgcgacg gcagcgaatc
     2941 ccgcgcgcct gcagtgtccg gacaataccg tcatatccct tgtcagcgga aacaatatgg
     3001 tattccttct ccggcccgta gtggcataca cactgcccca gctccagcac cagatgaaaa
     3061 tcaacattgt tccgggagac ttcactgaca tgccggagtg tgatttttac tgcttcaggg
     3121 aaagcgttta ctggcagtgc cacattattc tgtttcgcac ccgcgaatat aattaactcc
     3181 gtatatatat ttaaggaaat ttctgtcagg ttactgcagt tctcgtaatc gataaaggca
     3241 atcacctgtt gcttctctga ttttccggtc gccattttct cttcctgatt aacagacttc
     3301 acaaccagat aaaagagtca taaataacac ttaacatcgc ctgatattaa cagccgggca
     3361 acgtgatgtt attattttgc tctccttact gaatgcattt gtccctgaca ggacaacact
     3421 gaccgtcaca tgacggtctt ttttatccat ctgacagttt atggtcacaa aaaacattct
     3481 gacagatatt aaccacgtaa tgactgtgta ttatctttct tcccgcaacc tcccggcatt
     3541 aaccggtggt agcttcacag ggttgatgta cagagcattc attcaatgtc agtaatacgc
     3601 tatgaatgat ttttggcctc ctttgctcag gggaagtctt ttttttgccg ttttttttca
     3661 ttatctccgt cgctgacagc gttcggctct ctgccgtgca aactgctgcc agtctgtacg
     3721 gggagcaaca cgggcagcca ggagccacgc ttcagcggcc tccttccgga atcccctgac
     3781 ctccaggctg acggcacggc ttccggcacg ctgccagcgc cagacctgac cgttccgtga
     3841 actgtttctg aatccattca tctttatcac tcctctttaa cccgtcagta ttatggtgtt
     3901 aaataatcta ctgaattggc ttcctttcgg gtacggaagt tatttccttt taagccttat
     3961 ttccctgcct cctctctgat ggaggctttt ttttatttct catcaagcac ggcctgaagt
     4021 tctgccttta tccggttctg gcggcggatt ttatccagac gcgctgccgc atacgcttcc
     4081 tcctcctgag aaatatgctc cgtcacatac ccttccgtgt cataccggca ggcaccggct
     4141 ttcatggcac acagatagct ttctgaacgg gtgatggcct tcagcgccct gcgcagtttt
     4201 ttatgcgaga gcgggatatt ccgctgcgcc acgtcttcca gtaacacgtc ccggataccg
     4261 caggccagaa gccggggcgt gtcaccgtca aacaatcccg gccaccaggg tttcagggta
     4321 ttcacggcct catccagcgt gggcagattc agataaatgg acagtgcctg tctggcctgc
     4381 gctttttttg ccgccagctc tgcttcccgg gctgctttct cggccagctt ctgctttttc
     4441 accttccact ttggtggcgt ggtgacattg atgatggttt tccgcctgcg gacaggcgtc
     4501 tccccttccg ttttccgctt cagtgtcagt accggtcgct tctgctctgt catgctgtgt
     4561 tctgcctctg acgaatcatc ctcttgcggt caaagatacc acacccgtca cagcgccaga
     4621 acgcccagca cagccagatg acaggcgtag aataccggga aaaaatcccc gggccagaaa
     4681 cggggtaatg attttcctgc acacgacacc agaccaacgg tcagcaccgt catcacaaga
     4741 ccggccaccg ccgctgcatc actggtggca aggttaagcg ccggaataac agccagcagg
     4801 caggcgacca gcgccagacg ttccgctctg tcttccgccc ggtacagccg gtggctgact
     4861 gccagcatca gcagcccggc aatgccgtaa ctggtgccgg acaaaggccc ccacagcgcc
     4921 atcagaagaa tggccgctgc tgtacgccac ccactgcgcg tttcacacca cgtcagcacc
     4981 tgtgccgcca ctgcaaaggc aaacaggata ttcccctcat accagggaaa gcctgccaga
     5041 taatatgcga actgggcaat aatcccccat ccccacagcc ggttaatggc cggttgccgg
     5101 atatgcgcat gacgggacag attcagcccc cacaccaggg caaacagcgg aaaggctcca
     5161 cgcccggcca gaaacatcca ttcctgcttc agctggaata tcagattgat gtggtccagt
     5221 accatcagca ccagtgccac cgttttgatg atatcccgct gtccgggtga ccagaccagc
     5281 aacgactgca accaggtatc ggtccgggca gccagtgaat cgttacgtgt cgtgttcgtg
     5341 ttatccgttg tcatcagcgt ttgccttcct gtatcagtct ccgcccaggg ttttctcttt
     5401 ctgcaggtca cggaccatat cccgctccat ctgctgcaga cgctcctgca gcagattttc
     5461 ccgggcaacc tcccggaccg cttcccgctc ccgttgtggt tcacgcagca cactctccgg
     5521 cagcgcagtt tcccg
//
