LOCUS       NZ_CP095262             5596 bp    DNA     circular CON 05-JUN-2024
DEFINITION  Klebsiella pneumoniae strain 21080937 plasmid p21080937_9, complete
            sequence.
ACCESSION   NZ_CP095262
VERSION     NZ_CP095262.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27363256
            Assembly: GCF_022982615.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   He,Z.
  TITLE     Comparative Genomic Analyses of Klebsiella pneumoniae in Anhui,
            China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   He,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2022) School of life science, University of
            Science and Technology of China, 1129 Huizhou Ave, Hefei, Anhui
            23005, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.4.9b; SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022982615.1-RS_2024_06_05
            Annotation Date                   :: 06/05/2024 03:31:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,887
            CDSs (total)                      :: 5,766
            Genes (coding)                    :: 5,588
            CDSs (with protein)               :: 5,588
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 72 of 178
            Pseudo Genes (incomplete)         :: 118 of 178
            Pseudo Genes (internal stop)      :: 24 of 178
            Pseudo Genes (multiple problems)  :: 35 of 178
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095262.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="21080937"
                     /isolation_source="sputum"
                     /host="Homo"
                     /db_xref="taxon:573"
                     /plasmid="p21080937_9"
                     /geo_loc_name="China: Anhui"
                     /lat_lon="31.85777 N 117.291452 E"
                     /collection_date="2021-09"
                     /collected_by="Zhien He"
     gene            511..687
                     /locus_tag="MU320_RS29215"
                     /old_locus_tag="MU320_29215"
     CDS             511..687
                     /locus_tag="MU320_RS29215"
                     /old_locus_tag="MU320_29215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            677..1021
                     /locus_tag="MU320_RS29220"
                     /old_locus_tag="MU320_29220"
     CDS             677..1021
                     /locus_tag="MU320_RS29220"
                     /old_locus_tag="MU320_29220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1230..>1664
                     /locus_tag="MU320_RS29225"
                     /old_locus_tag="MU320_29225"
                     /pseudo=""
     CDS             1230..>1664
                     /locus_tag="MU320_RS29225"
                     /old_locus_tag="MU320_29225"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1699..2208
                     /locus_tag="MU320_RS29230"
                     /old_locus_tag="MU320_29230"
     CDS             1699..2208
                     /locus_tag="MU320_RS29230"
                     /old_locus_tag="MU320_29230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            2215..2427
                     /locus_tag="MU320_RS29235"
                     /old_locus_tag="MU320_29235"
     CDS             2215..2427
                     /locus_tag="MU320_RS29235"
                     /old_locus_tag="MU320_29235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3094..3318
                     /locus_tag="MU320_RS29240"
                     /old_locus_tag="MU320_29240"
     CDS             3094..3318
                     /locus_tag="MU320_RS29240"
                     /old_locus_tag="MU320_29240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            3302..3568
                     /locus_tag="MU320_RS29245"
                     /old_locus_tag="MU320_29245"
     CDS             3302..3568
                     /locus_tag="MU320_RS29245"
                     /old_locus_tag="MU320_29245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3638..3988)
                     /locus_tag="MU320_RS29250"
                     /old_locus_tag="MU320_29250"
     CDS             complement(3638..3988)
                     /locus_tag="MU320_RS29250"
                     /old_locus_tag="MU320_29250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(4054..4599)
                     /locus_tag="MU320_RS29255"
                     /old_locus_tag="MU320_29255"
     CDS             complement(4054..4599)
                     /locus_tag="MU320_RS29255"
                     /old_locus_tag="MU320_29255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(4625..4951)
                     /locus_tag="MU320_RS29260"
                     /old_locus_tag="MU320_29260"
     CDS             complement(4625..4951)
                     /locus_tag="MU320_RS29260"
                     /old_locus_tag="MU320_29260"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(5309..5467)
                     /locus_tag="MU320_RS29510"
     CDS             complement(5309..5467)
                     /locus_tag="MU320_RS29510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     Typing          346..425
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          5390..5579
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.67"
                     /coverage="94.0"
                     /evalue="3.08e-71"
     Typing          1012..2543
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggagagcgca cgagggagcc gccggagaga aatcaccggc atctttaagt cctgtcgggt
       61 ttcgtcctcc tctgacttga gcgtccattt ctgtgatgct cgtcagggga ggcggagcct
      121 atggaaaaac ggcaacgccg catccttttc tccggccctt tctgatttga gcgcctttgc
      181 tttatcccgt tatccgtgag tcggtcacgc ccgccgcagc cgaacggcag gagcgcagct
      241 accgagtgag cgaggaggcg tcatttcatc tgtccgtgca tggtattgcg gacgctcctg
      301 agccacatat cacacctgcc acatgaggca ctttcatgaa tcgtgctctc atgccaacat
      361 agtaagccag tatacacagc gtaaacgctg tgactggttc agggctgcgc cccgaaaccc
      421 gctaacacca ctgacgcgct ggcgcttgtc caacgccgga gcgacccgga aaacttttcc
      481 gtgccgtccg gcgtttgcga aggagggata atgcagaagc cgaagaagaa gccgctcgtc
      541 gtaaaaaaaa gatcctggca tccggcagtg atggcaaatc tgatgcgtca ggcacaggag
      601 gaagattacc gccgggagca gatggcagag cttgagcgtt cagcccggtc acattttgcg
      661 gatatcggag gagaggatgg cggttaagcg tacccgtttt ttaggtatcc gggtgaccga
      721 tggggagtac cagcagctgc tggagcgctg caatggaagg caactggcgg tgtggatgcg
      781 ggaaacctgt ctcgatacgc gcccggcgcg gtcgttgcgg ctgccgtcca tcgatccggt
      841 tttgctgcgc cagcttgccg ggatggggaa caacctcaac cagatcgccc ggaaaattaa
      901 cggcggccag tggtcaggcg ctgaccgggt tcaggtggtg gccgcgctga tggccattga
      961 tgccgaactc gagcggctgc ggcatgccgt gctggaaaag ggggcagacg atgatcgtta
     1021 agtttcatcc gcgagggcga ggcggcggtg ccgggccggt ggattatctg ctggggaaag
     1081 accgccagcg cgacggggcc atcgtcctgc agggaaagcc ggaggaggtc cgggagctta
     1141 tcgacgcctc gccctacgcc aaaaagtaca cctccggcgt cctgtctttt gctgaacagg
     1201 atttaccgcc cggccagcgt gaaaagctga tggcgagctt cgagcgggtt ctgatgcccg
     1261 gactcgataa agaccagtac agcgtgctgt gggttgaaca ccgggacaag gggcggctgg
     1321 aactgaattt tctggtaccg aacacggaac tgctgaccgg caggcgtctc cagccgtact
     1381 acgaccgggc agaccgtccg cgcatcgatg cgtggcagac cgtggtgaac gggcgactgg
     1441 ggctgcacga cccgaacgca ccggagaacc ggcgcgcgct ggtgacgcca tcttcgttac
     1501 ccaaaacgaa gcaggaagcc gcagaggcta ttacgcgggg cttactggcc ctcgcctcgt
     1561 caggggagct taaaacgcgt caggacgtca ctgaggcgct ggaaagcgca ggttttgagg
     1621 tcgtgcgcac cacaaagagc agcatcagca ttgccgaccc ggacggaggg cgaaacatcc
     1681 gacttaaggg agccatctat gaacagtctt ttaacgctgg cgaaggactt agagcagaaa
     1741 tcgaaagcgc agcagcagag taccggcgaa atgctgaaag ccgcattcag cgagcacgag
     1801 aagtctgtca gagcggaact gagcgaaagc gggaagagaa tcagcgccgc catccgcgcc
     1861 cacgagcagg gtatgagcgc ggccatgcag tcgaaccgcc tgagcgtgat gcgtatggtc
     1921 gggcggacat ggctgaccat cacgatggtg tcaggactgc tgttcgccag cctgagcggg
     1981 gtgttgtggt atcaggggag cttgatagcg tcaaatctgg ccgaaatcga ccggcagaac
     2041 gcagcgctgt cgaagctgaa cgcgaagacc tggggcgtga cgtacctgga agacagcaac
     2101 ggacgttttc tggtgctgcc gaaggggacg gccgtagaca ggacgcagag ctggacggtc
     2161 gggaacgggc ggtcgaagca gaacgcgctg agactggtga aggagtaagt gccgatgacc
     2221 gagctggaaa aacagttgct gagcgcatta gagcagctac agcaggacta ctcgaaaagg
     2281 ctggacgagt gggagagcgc cttcggggaa tggcggaaaa tgtcagggct tatgcaacgg
     2341 gagaacgcgg cgctgagcga gcgcgtgacg cgcttgagtc agcaggtgga acgcttgagc
     2401 gggcaactgc agcgtttgag ccggtagtgc agcgtcatga gatggccgtg gccgcagaac
     2461 gggcgcacga gcaacggcag cacgaaaagg aactgacgga agcgcgttcc cggcagcgaa
     2521 gctatgacgg tccgtcgctg ggctgaaatg caggaaaaca gggttctcat cgggcattta
     2581 cgctgaaacc ggatgtcatt cacctggctg catggctatg cagccaggta aaatttatct
     2641 gtcgcgaacg gggcggttta tcgggtggtt tgttgccggt tttacctgac tgccaccccg
     2701 ttcgcggcga acccgtccgg ggcggtgcgg gcaacgggtg ttatgtttaa tcagcgtgaa
     2761 cggcagatac aaaaaagccc cggagggctt ttaactactg acttattttt ttgaagattt
     2821 gctgggtttc aatttgtcag tatttcccgg cgttttttta ttgtcacgct ggcgtttatc
     2881 aggttcccct gttgaatcgg caattcgttt cggacccggc ttaagaccca taatcgttat
     2941 cctttcaata gttaagagga aagccctcaa gctagatata ttgaccgcgt tacggattat
     3001 caagaggggc taacaagatt attttttgtt cttttaggtt gattgtggta ctgttgatat
     3061 acatgtatag caaatggtgt tcgggaggtt tatatgcttg ctatccggtt atctgatgag
     3121 attgagtccc gtctggactc gctggcgaag caaaccggca gaacaaagac gttttatgcg
     3181 cgggaagcaa tactggcgca tctggaagac ctggaggatt attatctttc agcagaaact
     3241 gctgcacgcg ttcgccgtgg tgatgaagca gtgcattcgt ctgaagacgt gaggaagtca
     3301 cttggtctgg acgattaact attccgatcg ggcgctcaaa tcgttacgca agatggacaa
     3361 acagaacgca cgacggattg tggattttat gagtttacgc attgcagttg ctgccgatcc
     3421 tcgccagtca gggaagccgc tcaaaggtga gctgggcgag ttctggcgct atcgggtggg
     3481 agattatcgc gttctgtgtg agatccgaga tgacgagctt gttatccttg ccgccacgat
     3541 tggacatcgc cgcgaagttt acgactgaag cccgctatgc gggctttttt gatgccgaca
     3601 acaggtatta tggtaaatac tcgactaact acgtgttcta ctgtttgtcg gttttagtta
     3661 accagcaggt tgtcttaacg ataatgtagc cgttattttt aactaataag gtcatattgt
     3721 taacgtcagg cataataaaa gacattatgg cctcagagtt atcatcagaa gcagctctct
     3781 gcattactac ttcgattccc ttttctttga atctataagt atccagttgc ctattaaact
     3841 tagaaagacc tacaaccata cttagtttgt tatctttaac agtaagagat gcttcctgag
     3901 taccattaca tcggtagtaa gcggtgtaac cgtcagcaaa agatcgaccg gggaatataa
     3961 atgctaggaa cgcaattaac caatacatag ttgagaatct tggtattaca catttcacta
     4021 tcctctcttt catttttcgt tcaccatact ttgttatctg atttttaatt tttctatttt
     4081 ataaaccaag tctctaccaa aaaggcgaaa attatcctca aataataata attgtttaag
     4141 aacatccatg aaagcagctg cttcaccctc agttatggga gctactctat ataacatata
     4201 acctgagtta agggtttctt ttatactttc ttcaatgctc attataaata ttagtggatt
     4261 atcatttttc ttcccccatg ggtttctaat taatccttcg atgtactcag gtcctttgcg
     4321 tcctattgcc tgcgcaatat tttttatttc atctgcgtga gggctttgtt gtacagcttt
     4381 gtttatgcct gcttgagtga ttttcagaga ctctacaccg tgtatgttgt atgagataaa
     4441 atgtaataaa ttaagatgca atgcaattaa tccatttttg tttacatctg agtatttgga
     4501 taaaaaacgt aatgaagtgt taactctccc ttgaagccta acaattggga gtgctgcctt
     4561 gtatatttcc ttctccgtta aataccgttt aaaaaacata attctccgcc tccgtatagc
     4621 gtgcctattt tatttcgtcg taaaacttag ctctgaaata ttcctttaga accatcatgt
     4681 cattaataat tgaatctatc tcatccattt tttcttggta ttcttttaat ggtgaaggga
     4741 gcggtaactc aatatttgaa aagtgattgg cgagataggg atcaaggttt ctcatccatt
     4801 caggcttttc gccaatgcgt tcttcaactg aaagtgaacc tgataatctc tggttaataa
     4861 gatcacttag actcattttg gttttattcg aaagccgttg taaggattgc tttaatccta
     4921 tatctagcat gatatttgtt ctttctctca ttaccccccc ttgctattgc gttttttgtt
     4981 gcgcacaaag ctaccattgc gcattaagaa acgcaataaa aaaggaggga gggagtggtt
     5041 tgtacatgat gtgcagtgcg ctgagggcat cagtggcggg tgtttttccg tgggatggat
     5101 ggagctccgg ggataacaga actgacgatc atttaaagga tcttcttgag atcctttttt
     5161 tctgcgcgta atctcttgca ctgtaaacga aaaaaccgcc tggggaggcg gtttgatcga
     5221 aggttaagtc agttggggaa ctgcttaacc gggtaactgg gtatacgaga ccgcagatac
     5281 caaaactgtt ctttcagtgt agccgcagtt aggcacccac ttcaagacct ctcaatatct
     5341 ctctcgtata tatccagtta ccaatggctg ctgccagtgg cgtttttgcg tgtccgtccg
     5401 ggttggactc aagatgatag ttaccggaaa gggcgcagca gtcgggctga acggggggtt
     5461 cgtgcataca gcccagcttg gagcgaacga cctaaaccga gccgagatac cagcagcgtg
     5521 agctatgaga aagcgccacg cttcccgaaa gggaaaaagg cggaacaggt atccggtaaa
     5581 cggcagggtc ggaaca
//
