LOCUS       NZ_CP095261             9789 bp    DNA     circular CON 05-JUN-2024
DEFINITION  Klebsiella pneumoniae strain 21080937 plasmid p21080937_8, complete
            sequence.
ACCESSION   NZ_CP095261
VERSION     NZ_CP095261.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27363256
            Assembly: GCF_022982615.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9789)
  AUTHORS   He,Z.
  TITLE     Comparative Genomic Analyses of Klebsiella pneumoniae in Anhui,
            China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9789)
  AUTHORS   He,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2022) School of life science, University of
            Science and Technology of China, 1129 Huizhou Ave, Hefei, Anhui
            23005, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.4.9b; SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022982615.1-RS_2024_06_05
            Annotation Date                   :: 06/05/2024 03:31:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,887
            CDSs (total)                      :: 5,766
            Genes (coding)                    :: 5,588
            CDSs (with protein)               :: 5,588
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 72 of 178
            Pseudo Genes (incomplete)         :: 118 of 178
            Pseudo Genes (internal stop)      :: 24 of 178
            Pseudo Genes (multiple problems)  :: 35 of 178
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095261.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9789
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="21080937"
                     /isolation_source="sputum"
                     /host="Homo"
                     /db_xref="taxon:573"
                     /plasmid="p21080937_8"
                     /geo_loc_name="China: Anhui"
                     /lat_lon="31.85777 N 117.291452 E"
                     /collection_date="2021-09"
                     /collected_by="Zhien He"
     gene            <16..633
                     /locus_tag="MU320_RS29150"
                     /old_locus_tag="MU320_29150"
                     /pseudo=""
     CDS             <16..633
                     /locus_tag="MU320_RS29150"
                     /old_locus_tag="MU320_29150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413030.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /name="replication protein"
     gene            1167..1445
                     /locus_tag="MU320_RS29155"
                     /old_locus_tag="MU320_29155"
     CDS             1167..1445
                     /locus_tag="MU320_RS29155"
                     /old_locus_tag="MU320_29155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_013213990.1"
                     /translation="MIHTANRTFHQLYREWIRERREHMHNVLTWERDRYGARLVGLFYR
                     YCKVANPFPRCTLNTRINYRAHAVNLPDWPARSLELNKMWLSWREKK"
                     /name="hypothetical protein"
     gene            1556..1981
                     /locus_tag="MU320_RS29160"
                     /old_locus_tag="MU320_29160"
     CDS             1556..1981
                     /locus_tag="MU320_RS29160"
                     /old_locus_tag="MU320_29160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="WP_013213989.1"
                     /translation="MMQTELNPLICSLVATPRRMAAMPRYVGRFYVVFESMLYQQMKGL
                     CREYRGAYWLMWELSNGGFYMAPGRRDEMLNIEAMNYFSGQMSADAAGITACLYLYSHL
                     SFHTEGADQERFSRLYHSLRDWACEHDEKEAILAAID"
                     /name="antirestriction protein"
     gene            2109..2264
                     /locus_tag="MU320_RS29165"
                     /old_locus_tag="MU320_29165"
     CDS             2109..2264
                     /locus_tag="MU320_RS29165"
                     /old_locus_tag="MU320_29165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_013213988.1"
                     /translation="MNTDIVSLAKHIGDALTARRPVHMPRMKMRELGLLLIELKAYREA
                     AATVTH"
                     /name="hypothetical protein"
     gene            2310..2606
                     /locus_tag="MU320_RS29170"
                     /old_locus_tag="MU320_29170"
     CDS             2310..2606
                     /locus_tag="MU320_RS29170"
                     /old_locus_tag="MU320_29170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="WP_013213987.1"
                     /translation="MIRPETLRPFAEDWQAPTADEIKEVLELIRQRKGLSKPLSGVDVA
                     DLVGLPGERGSGKGTRTFRRWVSKTNPSPIAYGAWSILAHLAGFGAIWDADRD"
                     /name="transcriptional regulator"
     gene            <2710..3591
                     /locus_tag="MU320_RS29175"
                     /old_locus_tag="MU320_29175"
                     /pseudo=""
     CDS             <2710..3591
                     /locus_tag="MU320_RS29175"
                     /old_locus_tag="MU320_29175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152397.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISKpn6 family transposase"
                     /name="IS1182-like element ISKpn6 family transposase"
     gene            complement(3841..4722)
                     /locus_tag="MU320_RS29180"
                     /old_locus_tag="MU320_29180"
     CDS             complement(3841..4722)
                     /locus_tag="MU320_RS29180"
                     /old_locus_tag="MU320_29180"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /protein_id="WP_004199234.1"
                     /translation="MSLYRRLVLLSCLSWPLAGFSATALTNLVAEPFAKLEQDFGGSIG
                     VYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKNALVPW
                     SPISEKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIGDTTFRLDR
                     WELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNHRIRA
                     AVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKHSEAVIAAAA
                     RLALEGLGVNGQ"
                     /name="carbapenem-hydrolyzing class A beta-lactamase KPC-2"
     gene            complement(4998..5978)
                     /locus_tag="MU320_RS29185"
                     /old_locus_tag="MU320_29185"
     CDS             complement(4998..5978)
                     /locus_tag="MU320_RS29185"
                     /old_locus_tag="MU320_29185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213985.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481-like element ISKpn27 family transposase"
                     /protein_id="WP_013213985.1"
                     /translation="MTQALHSQARTTHLIREEIRNSTLPQAELARMYNVTRQTIRKWQN
                     RESPEDKSHAPNKMYTTLTPEQELIVVELRKTLLLPTDDLLAVTREFINPAVSRAGLGR
                     CLRRHGVSDLRNLVEQEGTAPATKKTFKDYEPGFVHIDIKYLPQMPDETARRYLFVAID
                     RATRWVFIELYADQTDGSSGDFLNKVQQACPVKIVKLLTDNGSQFTDRFTAGGKKKEPS
                     GTHVFDRLCKQLGIEHRLIPPRHPQTNGMVERFNGRISDIVNQTRFGSAAELESTLRNY
                     VKIYNHSIPQRALQHKTPVQALKEWHEKRPELFRKRVYNQPGLDI"
                     /name="IS481-like element ISKpn27 family transposase"
     gene            complement(6101..>6562)
                     /locus_tag="MU320_RS29190"
                     /old_locus_tag="MU320_29190"
                     /pseudo=""
     CDS             complement(6101..>6562)
                     /locus_tag="MU320_RS29190"
                     /old_locus_tag="MU320_29190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     gene            complement(6614..7318)
                     /locus_tag="MU320_RS29195"
                     /old_locus_tag="MU320_29195"
     CDS             complement(6614..7318)
                     /locus_tag="MU320_RS29195"
                     /old_locus_tag="MU320_29195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            <7383..7973
                     /locus_tag="MU320_RS29200"
                     /old_locus_tag="MU320_29200"
                     /pseudo=""
     CDS             <7383..7973
                     /locus_tag="MU320_RS29200"
                     /old_locus_tag="MU320_29200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            8172..>8666
                     /locus_tag="MU320_RS29205"
                     /old_locus_tag="MU320_29205"
                     /pseudo=""
     CDS             8172..>8666
                     /locus_tag="MU320_RS29205"
                     /old_locus_tag="MU320_29205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /name="IS6-like element IS26 family transposase"
     gene            8730..9434
                     /locus_tag="MU320_RS29210"
                     /old_locus_tag="MU320_29210"
     CDS             8730..9434
                     /locus_tag="MU320_RS29210"
                     /old_locus_tag="MU320_29210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     ARG             complement(3845..4722)
                     /name="blaKPC-2"
                     /gene="blaKPC-2"
                     /gene_name="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          74..633
                     /name="IncU"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attctgcggt tccccctgga agacctacgc aagttgggcc agctcagagg tggaatcaac
       61 gaaggcgagc gaatgaggca tcttttctgg cgtcttaact ttctccttct ctccggggca
      121 acaaacagcg cccagatgta ccatgaggcc gccgctcttt cctccgagct ggatactcgt
      181 tggtcttacc gctccaaaga gcttatgacg ctctacagca aggcgaaatc ctatgaggct
      241 ggggaacgtg tcgagttcgg cggaaagtcg tttgcaccgc tatacacgcc caaaaacgac
      301 acgctgatca acctattcca gatctcacag caagaacaag agcaactgaa gacgattatc
      361 agcactgacg aggctcagaa gcgccgcaga gagcgcgata gactgcgtga tgaagaacgt
      421 cgccgcgccg ctggccagct tgagcgtgaa gcctatgaag ccaactcatt gagcaggcaa
      481 aagccctggg aagctatggg catgagccgg gctaaatggt atcgcctggg caagccttca
      541 cctcaacaaa acagtgagac aagtccctcc cctattacta atggcgaagc ctcagcggtt
      601 gcccttcagg cggccaggtg taaagccgtt tagatggcta tttcaaagtg agtgagattt
      661 cgtaacggat ggcagcccta gaggctgctt tcttttgagg ggttagcagg cgtatagcca
      721 tgctggatct cagtctatca ctacctgctt aatattccac ctttagatcc ggcagcagtt
      781 cgaccaggtg gccaacttac caggcaccgg cagcagctcg accgggtggc caagttcaga
      841 tctggacgcc agaaggaaat caaccaggtg gccaacttac caggcaccgg cagcagctcg
      901 accaggtggc caagttcaga tctggacgcc agaaggaaat caaccaggtg gccaactcac
      961 caggcatcgg cagcagctcg accaggtggc ccatttcgta cgtacgaaat caggcgacgc
     1021 tatgcgaaat cttggcggca atcccccgcg actacgtgat ttttttaacg tagagcgcgc
     1081 tttttcttgc catagtaggc cattttggcc tataatctta attaagaaga ccgggcaccc
     1141 gccacggtca ataccggaga actccgatga tccacacagc taaccgcacc tttcaccaac
     1201 tctatcgaga atggatacgc gaacgccgcg agcatatgca caacgttttg acctgggagc
     1261 gtgatcgtta cggtgctcgc ctcgtcggtc tgttttatcg ttactgcaag gtggccaacc
     1321 cctttccccg ttgcactctg aatactcgga tcaactaccg cgcccatgcg gtgaatctgc
     1381 cagattggcc agcccgcagc ctggagctga ataaaatgtg gctcagttgg agagaaaaaa
     1441 agtaggccga tttggcctat tttttattgt acccgcttga cacaataggc caaaatggcc
     1501 tattatatat ctcagaaggc cgggcacccg ccacggtcaa taccggagaa ctccgatgat
     1561 gcaaacagaa cttaatcccc tgatctgctc cctggtcgcc acccctcgcc gtatggctgc
     1621 aatgccccgc tatgtaggcc gtttctatgt ggttttcgag tcgatgcttt accagcaaat
     1681 gaaagggctc tgccgcgagt atcgcggggc ttattggctt atgtgggagc tgtcgaacgg
     1741 cggcttttat atggctcctg ggcgtcgtga tgaaatgctg aacatcgagg ccatgaacta
     1801 ctttagtggc caaatgagcg ccgatgctgc cgggattacc gcttgcctct acctctatag
     1861 ccatctgtca tttcacacag aaggcgctga ccaggagcgg ttttcaaggc tgtatcacag
     1921 cttgcgggat tgggcttgtg agcatgacga aaaagaggcc atcttggccg caatagacta
     1981 gcaacagaaa gccccggtga tggccggggc tcttaaagga ggtgcatagg tagaaaaaga
     2041 aatggtgccc ctggtatggc tgcaaccaat cacaggggct ttcaccggtc aatttgagga
     2101 taaacgcgat gaatacggat attgtatcac ttgcaaaaca cattggcgac gctctgacgg
     2161 cccgccgccc cgttcatatg ccgcgcatga agatgcggga gcttggcttg ctcttgattg
     2221 aactgaaggc gtatcgggag gccgccgcga ccgttaccca ttgataaaaa agccccggca
     2281 ttgaccgggg ctatttgagg atttatcgca tgattagacc tgaaacgctt aggccgttcg
     2341 ctgaagattg gcaggctcca accgccgatg aaatcaaaga ggtgttggag ctgattaggc
     2401 aacgaaaagg gcttagtaag cctctcagtg gcgttgatgt cgctgatctc gttggtttgc
     2461 ctggtgagag aggcagcgga aagggaaccc gaaccttcag gcgttgggtg agcaaaacca
     2521 accctagccc tattgcttat ggtgcctggt ctatcttggc ccacctggca ggtttcgggg
     2581 ctatatggga cgctgaccgg gattgaaacc atgaccgaac ataaggccga acgtgctccc
     2641 tggggagact tcccggcagt agtgaggaac ggcgatctaa aggatctctc taaagaacct
     2701 gaatacgaag ccgccaaaca tggcgaccac aaggcgatga gctataagcg catgaagccg
     2761 gccgaggacg aattgcattg cgagatcaag gcgctgcttg atcgcgccaa ggctaccgac
     2821 gaccaggagc gtaacgagcc ggagctggac attcctgccg agatttctcg ccgcgagaag
     2881 cgcctggagg cgatccaggc ggcaaaggcg cgcctggaag cgcgccagcg tgaagcggac
     2941 caggcccggg ggcgcagcga agacgatggc cgccggcctc gccatccgga tggctcggac
     3001 aagggcggtg gctcgtacaa acgcgagttt ggtgtgccgg atgaccgtga tcaggaaagc
     3061 ttcaccgatc cggacagccg gatcatgaaa cacgccggtg gtggctccga gcagagctac
     3121 aacgggtaca cagcggtcga tgccgagcac cagatcatcg tggcggcgga gttgaccaac
     3181 tgcgccgcgg acagtcaggc gctgctgggc atgctggcag cagttcaggc caacaccgga
     3241 gaaatgccgg cccagacgct ggcggatgcg ggattccgta gtgaggctgt tctggcaaag
     3301 gtcgccgatc accacggcga tgtcatcgtt gccctcggcc gcgagggacg tgaagatgcc
     3361 aaggtcaatg ccaagaccca tccgcatacg gcggcgattg cggcgaaatt gaaaacggag
     3421 caaggcgatg cagcttaccg ccggcgcaag tcgatcgtgg aggctccgaa tggttggatc
     3481 aaggcggtga tgggattgcg tcagttcagc atgaggggcc tggacaaggt gcaagccgag
     3541 tggaagctcg tctgcatggc gctaaatctg aggcgaatgg cgtatctgtg agggcgaagg
     3601 ttaaatgggg cggctcaaat gcaccccagt cgtcataaca cgccgcgcgc cgcaagattg
     3661 gtatccttgg cgccgacgcc ttgccaattg cagagagcgc cgccgccatc gtcagtgctc
     3721 tacagaaaac cggtcacacg gctctgccgc gcagactcct agcctaaatg tgacagtggt
     3781 tggtaatcca tgccgcccgc aagggcggcg gtggtgggcc aatagatgat tttcagagcc
     3841 ttactgcccg ttgacgccca atccctcgag cgcgagtcta gccgcagcgg cgatgacggc
     3901 ctcgctgtgc ttgtcatcct tgttaggcgc ccgggtgtag acggccaaca caataggtgc
     3961 gcgcccagtg ggccagacga cggcatagtc atttgccgtg ccatacactc cgcaggttcc
     4021 ggttttgtct ccgactgccc agtctgccgg caccgccgcg cggatgcggt ggttgccggt
     4081 cgtgtttccc tttagccaat caacaaactg ctgccgctgc ggcgcagcca gtgcagagcc
     4141 cagtgtcagt ttttgtaagc tttccgtcac ggcgcgcggc gatgaggtat cgcgcgcatc
     4201 gcctgggatg gcggagttca gctccagctc ccagcggtcc agacggaacg tggtatcgcc
     4261 gatagagcgc atgaaggccg tcagcccggc cgggccgccc aactccttca gcaacaaatt
     4321 ggcggcggcg ttatcactgt attgcacggc ggccgcggac agctccgcca ccgtcatgcc
     4381 tgttgtcaga tatttttccg agatgggtga ccacggaacc agcgcatttt tgccgtaacg
     4441 gatgggtgtg tccagcaagc cggcctgctg ctggctgcga gccagcacag cggcagcaag
     4501 aaagcccttg aatgagctgc acagtgggaa gcgctcctca gcgcggtaac ttacagttgc
     4561 gcctgagccg gtatccatcg cgtacacacc gatggagccg ccaaagtcct gttcgagttt
     4621 agcgaatggt tccgcgacga ggttggtcag cgcggtggca gaaaagccag ccagcggcca
     4681 tgagagacaa gacagcagaa ctagacggcg atacagtgac atcaacgata ttccttgttt
     4741 gaaggtggag ctaggtgtag cggccggatg taatcaggcc gctacagctg ggattagggg
     4801 tctgacgctc agtggaacga aaactcacgt taagaagtca tttttcaata ttattgaagc
     4861 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     4921 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     4981 attatcatga cattaaccta tatgtcaaga cccggctggt tatacacgcg tttcctgaac
     5041 aattcagggc gtttttcatg ccattccttg agcgcctgaa cgggtgtttt gtgttggagc
     5101 gcgcgttgcg gaatgctgtg gttgtagatc ttgacgtaat tgcgcagcgt cgattccagt
     5161 tcggcagctg aaccaaaacg ggtctggttg acgatgtcgc tgatacgacc gttgaagcgc
     5221 tccaccatgc cgttggtctg cggatgacga ggcgggatga gccggtgctc gatgccgagc
     5281 tgcttgcaca ggcggtcgaa cacgtgtgtg ccgctgggtt ccttcttctt gccgccagcc
     5341 gtgaagcggt cggtgaactg gctgccgttg tcggtcagaa gcttgacgat cttgacggga
     5401 caggcttgct ggactttgtt gaggaagtcg ccactgctgc catcggtctg gtcggcatag
     5461 agctcgatga agacccagcg cgtagcacgg tcgatggcaa cgaagagata gcgccttgcc
     5521 gtctcgtcgg gcatctgcgg caggtacttg atgtcgatgt gcacaaagcc cggctcgtag
     5581 tccttgaagg tctttttcgt ggcgggcgca gtgccttcct gctcgaccag gttacgtaga
     5641 tccgagacac cgtggcggcg caggcaacgt cccaggccgg cacgcgagac ggctggattg
     5701 atgaactcgc gcgtgaccgc cagcaggtcg tccgtgggca gcagcaacgt cttgcgcagc
     5761 tccaccacga tgagctcctg ctcgggcgtg agcgtcgtgt acatcttgtt cggcgcatgc
     5821 gacttgtctt caggagactc gcggttttgc cactttcgga tggtttggcg ggtgacgttg
     5881 tacatcctgg ccagttcggc ctgcgggagc gtcgagttcc tgatttcctc acggatcagg
     5941 tgggtagtac gggcttggct gtgcagtgcc tgggtcatcg agttgccagt gaagagtcgg
     6001 attggaacag tgtacgcaaa acgtgtctgg ccttgaagag cggccagcta cgcatgggta
     6061 catgatccca cgagtccaga cacctataaa aataggcgta tcacgaggcc ctttcgtctt
     6121 caagaatttt ataaaccgtg gagcgggcaa tactgagctg atgagcaatt tccgttgcac
     6181 cagtgccctt ctgatgaagc gtcagcacga cgttcctgtc cacggtacgc ctgcggccaa
     6241 atttgattcc tttcagcttt gcttcctgtc ggccctcatt cgtgcgctct aggatcctcc
     6301 ggcgttcagc ttgtgccaca gccgacagga tggtgaccac catttgcccc atatcaccgt
     6361 cggtactgat cccgtcgtca ataaaccgaa ccgcgacacc ctgagcatca aactctttta
     6421 tcagttggat catgtcggcg gtgtcgcggc caagacggtc gagcttcttc accagaatga
     6481 catcaccttc ctccaccttc atcctcagca aatccagccc ttcccgatct gttgaactgc
     6541 cggatgcctt gtcggtaaag aggcactgtt gcaaagttag cgatgaggca gccttttgtc
     6601 ttattcaaag gccttacatt tcaaaaactc tgcttaccag gcgcatttcg cccaggggat
     6661 caccataata aaatgctgag gcctggcctt tgcgtagtgc acgcatcacc tcaatacctt
     6721 tgatggtggc gtaagccgtc ttcatggatt taaatcccag cgtggcgccg attatccgtt
     6781 tcagtttgcc atgatcgcat tcaatcacgt tgttccggta cttaatctgt cggtgttcaa
     6841 cgtcagacgg gcaccggcct tcgcgtttga gcagagcaag cgcgcgacca taggcgggcg
     6901 ctttatccgt gttgatgaat cgcgggatct gccacttctt cacgttgttg aggattttac
     6961 ccagaaaccg gtatgcagct ttgctgttac gacgggagga gagataaaaa tcgacagtgc
     7021 ggccccggct gtcgacggcc cggtacagat acgcccagcg gccattgacc ttcacgtagg
     7081 tttcatccat gtgccacggg caaagatcgg aagggttacg ccagtaccag cgcagccgtt
     7141 tttccatttc aggcgcataa cgctgaaccc agcggtaaat cgtggagtga tcgacattca
     7201 ctccgcgttc agccagcatc tcctgcagct cacggtaact gatgccgtat ttgcagtacc
     7261 agcgtacggc ccacagaatg atgtcacgct gaaaatgccg gcctttgaat gggttcatgt
     7321 gcagctccat cagcaaaagg ggatgataag tttatcacca ccgactattt gcaacagtgc
     7381 cgtcagaggg gatggcaatg ccggagtcgt tgtcacatat cacgacggaa tcacagtgga
     7441 gaagcctggt gctgaaatcc ggcggaaaat actggcatgt gtacatgtcg aagaagacag
     7501 ccggagggcg gaatacggca cgctacctgg gtcgttatct gaagaagcca ccaatagcgg
     7561 cttcccgact ggctcattac aacggagggg caagcctgag cttccgttac ctggaccaca
     7621 aaacgggaga aacggcgacg gaaacgctga cacagcgtga gctggtcgcg aggctgaaac
     7681 agcacatccc ggagaagttt tttaagatgg tgaggtactt cgggttcctt gccaaccgtg
     7741 tgtgtggaga gaagctgccg caggtgtacc gtgcactggg gatggataaa ccggaaccag
     7801 tggcgaaagt gtgctatgca caaatggtga aacagttcct gagtcgtgac ccgttcgaat
     7861 gcgtgctgtg tggctgccgg atggtatacc gccgggccat tgcggggctg aatgtatcag
     7921 ggctgaagaa aaacgcgcgg gatatcagtc tactgaggta tatgccggcc tgacacaggc
     7981 taagtgtgcc cgggaatcgg tgaaagtata aaaaacagcc tgttacggta taaaaatcga
     8041 ccatacagag agggaaacca ccggagtggt gggaaaagca gaaaggctca cgtggcataa
     8101 ggcgtggagg cactgttgca aatagtcggt ggtgataaac ttatcatccc cttttgctga
     8161 tggagctgca catgaaccca ttcaaaggcc ggcattttca gcgtgacatc attctgtggg
     8221 ccgtacgctg gtactgcaaa tacggcatca gttaccgtga gctgcaggag atgctggctg
     8281 aacgcggagt gaatgtcgat cactccacga tttaccgctg ggttcagcgt tatgcgcctg
     8341 aaatggaaaa acggctgcgc tggtactggc gtaacccttc cgatctttgc ccgtggcaca
     8401 tggatgaaac ctacgtgaag gtcaatggcc gctgggcgta tctgtaccgg gccgtcgaca
     8461 gccggggccg cactgtcgat ttttatctct cctcccgtcg taacagcaaa gctgcatacc
     8521 ggtttctggg taaaatcctc aacaacgtga agaagtggca gatcccgcga ttcatcaaca
     8581 cggataaagc gcccgcctat ggtcgcgcgc ttgctctgct caaacgcgaa ggccggtgcc
     8641 cgtctgacgt tgaacaccga cagattggca ctgttgcaaa tagtcggtgg tgataaactt
     8701 atcatcccct tttgctgatg gagctgcaca tgaacccatt caaaggccgg cattttcagc
     8761 gtgacatcat tctgtgggcc gtacgctggt actgcaaata cggcatcagt taccgtgagc
     8821 tgcaggagat gctggctgaa cgcggagtga atgtcgatca ctccacgatt taccgctggg
     8881 ttcagcgtta tgcgcctgaa atggaaaaac ggctgcgctg gtactggcgt aacccttccg
     8941 atctttgccc gtggcacatg gatgaaacct acgtgaaggt caatggccgc tgggcgtatc
     9001 tgtaccgggc cgtcgacagc cggggccgca ctgtcgattt ttatctctcc tcccgtcgta
     9061 acagcaaagc tgcataccgg tttctgggta aaatcctcaa caacgtgaag aagtggcaga
     9121 tcccgcgatt catcaacacg gataaagcgc ccgcctatgg tcgcgcgctt gctctgctca
     9181 aacgcgaagg ccggtgcccg tctgacgttg aacaccgaca gattaagtac cggaacaacg
     9241 tgattgaatg cgatcatggc aaactgaaac ggataatcgg cgccacgctg ggatttaaat
     9301 ccatgaagac ggcttacgcc accatcaaag gtattgaggt gatgcgtgca ctacgcaaag
     9361 gccaggcctc agcattttat tatggtgatc ccctgggcga aatgcgcctg gtaagcagag
     9421 tttttgaaat gtaaggcctt tgaataagac aaaaggctgc ctcatcgcta actttgcaac
     9481 agtgccctga aatctataac cttcgcgggc atgtgtcaaa aaatgggaaa gcagactcta
     9541 ttctgacgaa gcggcgcggc cctgcctgac atcaagttag ggtatagcct agattgacat
     9601 gcgcgatgca acccttaact tgcttgcacc tatcgtttcc atgctagctt tatcgtaacg
     9661 ctaaggaagc tctgaaaaag cccatgtttc gcgaaatcac cgctctgtaa cccgcatggt
     9721 tgctgggatg catttctcga gcgggggctt tttcagacct tccttagctt agcgtacgat
     9781 tttttccga
//
