LOCUS       NZ_CP095259            11970 bp    DNA     circular CON 05-JUN-2024
DEFINITION  Klebsiella pneumoniae strain 21080937 plasmid p21080937_6, complete
            sequence.
ACCESSION   NZ_CP095259
VERSION     NZ_CP095259.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27363256
            Assembly: GCF_022982615.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   He,Z.
  TITLE     Comparative Genomic Analyses of Klebsiella pneumoniae in Anhui,
            China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   He,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2022) School of life science, University of
            Science and Technology of China, 1129 Huizhou Ave, Hefei, Anhui
            23005, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.4.9b; SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022982615.1-RS_2024_06_05
            Annotation Date                   :: 06/05/2024 03:31:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,887
            CDSs (total)                      :: 5,766
            Genes (coding)                    :: 5,588
            CDSs (with protein)               :: 5,588
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 72 of 178
            Pseudo Genes (incomplete)         :: 118 of 178
            Pseudo Genes (internal stop)      :: 24 of 178
            Pseudo Genes (multiple problems)  :: 35 of 178
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095259.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="21080937"
                     /isolation_source="sputum"
                     /host="Homo"
                     /db_xref="taxon:573"
                     /plasmid="p21080937_6"
                     /geo_loc_name="China: Anhui"
                     /lat_lon="31.85777 N 117.291452 E"
                     /collection_date="2021-09"
                     /collected_by="Zhien He"
     gene            complement(join(11263..11970,1..24))
                     /gene="mobC"
                     /locus_tag="MU320_RS28965"
                     /old_locus_tag="MU320_28965"
     CDS             complement(join(11263..11970,1..24))
                     /gene="mobC"
                     /locus_tag="MU320_RS28965"
                     /old_locus_tag="MU320_28965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(24..1988)
                     /locus_tag="MU320_RS28970"
                     /old_locus_tag="MU320_28970"
     CDS             complement(24..1988)
                     /locus_tag="MU320_RS28970"
                     /old_locus_tag="MU320_28970"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3472..3621)
                     /locus_tag="MU320_RS28975"
                     /old_locus_tag="MU320_28975"
     CDS             complement(3472..3621)
                     /locus_tag="MU320_RS28975"
                     /old_locus_tag="MU320_28975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3706..3963)
                     /locus_tag="MU320_RS28980"
                     /old_locus_tag="MU320_28980"
     CDS             complement(3706..3963)
                     /locus_tag="MU320_RS28980"
                     /old_locus_tag="MU320_28980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3973..5658)
                     /locus_tag="MU320_RS28985"
                     /old_locus_tag="MU320_28985"
     CDS             complement(3973..5658)
                     /locus_tag="MU320_RS28985"
                     /old_locus_tag="MU320_28985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(5763..7250)
                     /gene="ltrA"
                     /locus_tag="MU320_RS28990"
                     /old_locus_tag="MU320_28990"
     CDS             complement(5763..7250)
                     /gene="ltrA"
                     /locus_tag="MU320_RS28990"
                     /old_locus_tag="MU320_28990"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            7895..8140
                     /locus_tag="MU320_RS28995"
                     /old_locus_tag="MU320_28995"
     CDS             7895..8140
                     /locus_tag="MU320_RS28995"
                     /old_locus_tag="MU320_28995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8414..8785
                     /gene="tolA"
                     /locus_tag="MU320_RS29000"
                     /old_locus_tag="MU320_29000"
     CDS             8414..8785
                     /gene="tolA"
                     /locus_tag="MU320_RS29000"
                     /old_locus_tag="MU320_29000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            8782..9147
                     /locus_tag="MU320_RS29005"
                     /old_locus_tag="MU320_29005"
     CDS             8782..9147
                     /locus_tag="MU320_RS29005"
                     /old_locus_tag="MU320_29005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            9322..9600
                     /locus_tag="MU320_RS29010"
                     /old_locus_tag="MU320_29010"
     CDS             9322..9600
                     /locus_tag="MU320_RS29010"
                     /old_locus_tag="MU320_29010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            9588..9902
                     /locus_tag="MU320_RS29015"
                     /old_locus_tag="MU320_29015"
     CDS             9588..9902
                     /locus_tag="MU320_RS29015"
                     /old_locus_tag="MU320_29015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            10066..10494
                     /locus_tag="MU320_RS29020"
                     /old_locus_tag="MU320_29020"
     CDS             10066..10494
                     /locus_tag="MU320_RS29020"
                     /old_locus_tag="MU320_29020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            10520..10699
                     /locus_tag="MU320_RS29025"
                     /old_locus_tag="MU320_29025"
     CDS             10520..10699
                     /locus_tag="MU320_RS29025"
                     /old_locus_tag="MU320_29025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(10726..11256)
                     /locus_tag="MU320_RS29030"
                     /old_locus_tag="MU320_29030"
     CDS             complement(10726..11256)
                     /locus_tag="MU320_RS29030"
                     /old_locus_tag="MU320_29030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     BGC             1..10658
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2791..3369
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11267..11970)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.043"
                     /coverage="97.0"
                     /evalue="3.82e-136"
ORIGIN
        1 gacgttgtac tgctccagtg tcatcagtaa agccccgcaa aatcatcatc cgcttttggt
       61 ccgccgccgt cggccggagg caggtactcc gccagcgggt ccgttttcgc cagcaccggg
      121 aaggttttca gcgaaatttc ccggcgctca acccgcagcg ggctgctgaa cgccagccgc
      181 gccacgccca gcccggtcat caccgcgaac ccgtcaggca ggtgttgcag ggtgttcacg
      241 tcaaacagcg gccgcgcctc cttctggatg aagctgtcgc cgctgatgtg ctccacgttc
      301 cccgcctccc ggctggcccg gcggcgctcc acgtccaccc ggatctcccc ggtctggccc
      361 gccgcccact gcgcggactc ctggctggcc gcgcgataga accagcggat cggcgtgtta
      421 tccagtaccg tggttttcac aaaatcagcg ggcagatcct gcccgcactg accgaagtcg
      481 cccagcgact ggtgcgcgag cagcaggttg cagtcccggc tgcgcagcgt ccccagcgcg
      541 ttcagcacgt acttcgacag cagatattta atctcatcga gcatgatgct cgcgtgcggc
      601 cacctccgga actcatcccg cgcaatgatg atttgcgcgc agcgggcaaa cagcatcttc
      661 tgcacccgga tcaccgcctc atcgtccatc gagccgatga cgtacaggca gccgccgccg
      721 ttgattatcc ccgccacatc gataccgctg tccgtctgca ggacgctcag gtcggccacg
      781 ttctccagct tggtgatcag ccccttcacg tcctttttca gggcctccgg caacgcgtac
      841 gccgccgcca gcacctgctg aatgtttgcc ccctgcggga actgctccgc aatcagcttc
      901 gccgctttct gctcgctgat gcggtagtgg tccgccacgt cgccggtctc actcaggtta
      961 aacccggcaa ccagcagatt cttcagggcg tactggtcga gatcgcggaa cagattcagc
     1021 tgcgccggtt tccccggccg cagatccagc agggtgaagt ccgggcagtg cgcccggaag
     1081 acgtgcggcg cccacgcgtc ccctttgggg tcgaagtaca ccacagcgtc cccgaaatcg
     1141 cgtacgcagc gaatgagcgc gttggtggcc atcgcgctct tgccgctccc gggcaggccg
     1201 aggatctgaa tattggtctt gcgccaggta tcacgaggga ccagcaccgg ctggccctgc
     1261 tcatccttac ccagaaacac cgcctgctgt ttccgggcct gctccgtgga aaaatcggcc
     1321 agcgcgttaa ccggtaccgg ctccccgaaa tgcaggtggc ggacgtctgt catttctccg
     1381 gtccgctggc tgctgcgccg gctctcctga ttcagtttcg cgttcaccgc ctcgtcagcg
     1441 gccgcaaaat gaccatcaag cagcaggaac gccagcagcc gcgccagaag gacgccggcg
     1501 acaagccctc cggcacacca ggctatcccg aacaggttag ccggctgcag caggtaataa
     1561 tctgcgttcg catagaggtc cgggcgcagg acggtttctc cccagctctc gataaacacg
     1621 gcgacaacca gcccccccag aagggccaga ccggcagcgc cggtcagcca gtacgcgtag
     1681 ttccagacat gtcgccgtgc cctgaccatc cgcccgggtg aatccagcga ggccagcacc
     1741 gaaaatggca cagtccggtt attatcgcgc ttatagcgat cggcatactc ccgccgcgcc
     1801 ttatggctca gccccacaaa acgcattcgg ccggcccaga gttcagccag accgaaaaac
     1861 aggccgataa ccagaaaaaa accggccaga ctgtaatact cccggtacag ccagctgacc
     1921 cacgggtaaa tctgccgggc cagctccggg atatgccact gccaggcaag cgaatccgta
     1981 ttaaacatgc aatctctctc cttcagacga cgggtttacc ggcgtgatgc gcagcccccg
     2041 ctgcacaagc cgcggacatg ccccaaggca tggcggcggc ggtcacgtcc gtggtgattg
     2101 cgttcagctt tcaccgcgga ggccagcctc tttccgcccc atgcggaaaa ggcgcagccc
     2161 cggaggaaca acgagcacgc cccagcgtgt gagaggaaaa ggcggcaccc cagtgcggtc
     2221 ttttccggtg ttccggcccc cgtcagagtt cgccatagcg agcggctgac cacccgcaaa
     2281 atcaggagca gctttgctgc ttcgtcaggt gcgtccagca ccgtggattt ttgtgggtgg
     2341 tcaggcgtga gcctgacggg ggccgggtga atttctggcg tggccggcgt ccagccggac
     2401 agagccagaa agaggggaaa agaccgtgcg tcggtggcgt ccagccagcg taagcaaggt
     2461 cttttcccct gaagtcagcc acgccccagc ggggatgact tgcatttctc cgcaaggaga
     2521 aaaaagcaac agagataggt taacgtaaaa tgttcagcga aaaaccgaca tggttcacct
     2581 atcctgataa ttgatcgtca gggaatagaa agacttaatc aggggacaat atcccacatc
     2641 agcgcgtccg gtgaccgtct tgcaggtgcc ggggaaattg cgcctcctcg ctcactcggt
     2701 cgctacgctc cgggcgtgag actgcggcgg gcgctgcgga cacatacaaa gttacccaca
     2761 gattccgtgg ataagcaggg gactaacatg tgaggcaaaa cggcagggcc gcgccggtgg
     2821 cgtttttcca taggctccgc cctcctgcca gagttcacat aaacagacgc tttttcggtg
     2881 catctgtggg agccgtgagg ctcaaccatg aatctgacag tacgggcgaa acccgacagg
     2941 acttaaagat ccccaccgtt tccggtaggt cgctccctct tgcgctctcc tgttccgacc
     3001 ctgccgttta ccggatacct gtccgccgtt ctcccttgcg ggaagtgtgg cgctttctca
     3061 tagctcacac actggtatct cggctcggtg taggtcgttc gctccaagct gggctgtaag
     3121 caagaactcc ccgttcagcc cgactgctgc gccttatccg gtaactgttc acttgagtcc
     3181 aacccgggaa agcacgcaaa acgccactgg cagcagccat tggtaactgg gagttcgcag
     3241 aggatttgtt tagctaaaca cgcggttgct cttgaagtgt gcgccaaagt ccggctacac
     3301 tggaaggaca gatttggttg ctgtgctctg cgaaagccag ttaccacggt taagcagttc
     3361 cccaactgac ttaaccttcg atcaaaccac ctccccaggt ggttttttcg tttacagggc
     3421 aaaagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctactgaac
     3481 cgcgatcccc gtcagttcag aggaggacga tggtgccacc gttccgccct gaacatcccg
     3541 gatataattt gcctgacatg cggccagaag aaagccagca acaatcaata taaaaagaaa
     3601 aatagtcttt acctttttca tatactttcc ctgtatttaa agactccggc attcatccct
     3661 gaacaccgga agtcagtatt tcaaatcatc cttgataatt ttttgttacc agttaccgtc
     3721 ccgataatca aaagctatat aacaattatc cacatcaggt tttatttgat ttttaaaaaa
     3781 tggctgtagt aaaggaaccc attctttctt caaatcaaac tcaccattat taatattatc
     3841 ctttaaaggc attcccaagc tttcaatgac agaaccatcg tcaccaaagt ctttagaata
     3901 ctcttcccct ttaaactctt cagttttctt atcaaaccat gtcaaacgta atttaagccc
     3961 catatttacc tctcaaagat attttttaat gttccgtttt ggatctggcc ctttaacctg
     4021 cttacccgtt ttgggatcaa atgcgcctag gtgttcgcca tcactggcac gatacccttc
     4081 aagctcaccg tgctgggaat cccattcata aattttacgt tttttatcgc cataccatcg
     4141 ggcccgctta ccaccaccac cttgtttagg tgttttagga tcacctcgct ttaactctcc
     4201 aagtcccttt atgtcttctg ttttaggtac tggatgataa tcatggccgt agtctttagt
     4261 ccccttgcga ggcttgttct tttcctcatt taacttattt tctgcatcct tcgctttttt
     4321 ctccttttgt ttccggctct ccatcgcccc ggtaagtgca gccttatctc tggataagga
     4381 ttcattcatt gcgtttaaag atgcctgagc cgcttttaat ttctcattct gattctgaat
     4441 atccttatca agatagcgaa cctgatcctg ccacccggag ccatattcat gaggcgggtt
     4501 ataagtcaca aaatcatctt tatttgcctc tgcctctttt acctttttat cggcttcctg
     4561 aacagcagcc cttgcaccgg ggagcgcctt ctctgtcgat gcaatgcggc tgtttaaggt
     4621 cgcaatattt ttatcttcgg catccagctc agctttcgct ttatcatagt ctcttttcag
     4681 cccctcttcc ggatgagcgg cgtcccatgc ctgctggcga cgcttttctt cctcctgacg
     4741 ctgttttacc tgtgccgggg taagaacatc tgtcaccgac acataaaccg gcttctgtcc
     4801 ggtctctttc gggaaacgaa taacggcctc acgggagtta ccaccggctg taaaaccagc
     4861 cggacgtgaa tcaccttttt cagcaacaat gccttttggc ggggctttcg ctgtcggaac
     4921 acctttcggt acgctcacct gcagagacgg gagacccgga atcgacacac tgaatacccc
     4981 cggacgtttt gtcggtttcg catccaccac agggacactc attggccgac ctgcgacaac
     5041 cgcaatatgc tgccgctcat ccttcaccac atccacaacc cgttgtctga cacgaaccgt
     5101 cgcctggtcc agtggtaaag tacttgccgg agtctccgtc accgcatcgg ccggtaatga
     5161 cgtcataatt tttgacatca ttttcggatc atcttttgca atctcagaag gcagcacacc
     5221 gtagatcgca atcccccaca gaccaaactt aaacggcccc ttcagggcag ccagcacatc
     5281 agcaacggcg gccgacaacg catcgccgga aactgataaa gccagtcctt cagcccccgg
     5341 agtagccagg gcaggcagac cgaaggccat cgcagtggcc gaagactgtc cgccaccaga
     5401 gctaccgcta tgattgccgc tgttaccacc attaccaccg ggtttactcc cgccgttacc
     5461 aaactccccc ggattatagt tatgaatatc tccattcggc gtatgcgtcg taccccagcc
     5521 cggcccgctg tttggatccg taccaccacc tgatgaaggg ccacctttac cagacgtacc
     5581 gttcacattc ccactggcct gaccaccatt atgtcccaga cctgaattac ccggacctcg
     5641 accgtctcca ccactcatac cgttttcctc tttgacattt aaaacaataa attaaagcca
     5701 acatgtcgcg gtagggatac ccgttaccgg ataccccccg cacagatccc ggcgtgcgcg
     5761 atttacgcac cgggctcctg cctcgggtgt ctggcggtga accgctccac aggccatgga
     5821 tgaagaaccc gaacccttgg tagccatgcg gctgccagtt tgtttgcttt cgtccaggtc
     5881 gtatcatcct tctggctcct gcgcctgagc gcccggcgcc agaggtttgt tacgtgtgtc
     5941 ctgaacttct gcatggtggg gaagttgccc ggtaccgagt gatagttcag gtatccctga
     6001 accactctcc tgagccattt tccctgttcg gggattgagt aatgccagcg ccttcgcaga
     6061 ccgtctttga tggctttcag agttgccgtc atccgatccc ggcgggtctt tcgtatcagc
     6121 atgaacctgc cgttgcgatc tttcccgctg atgtgcgtga acccgaggaa gttgaacgtt
     6181 tctggtttgc cttttcccct gatggcacgg ttttcggcag cgaagcggcc gaactccatc
     6241 agacgggttt tctccgggtg aaccgtgagt ccgaactccc tcagtctgcg ctgcatggct
     6301 atacggaagc gccgggcatc gtatcgtttg tcgaacccga tgacgatgtc atcggcgtat
     6361 ctgaccatta ccacattgcc tgtggcatag cgacgtcgcc actgatgcgc ccacagatcg
     6421 aagacgtagt ggaggtatat gtttgccagc agcggtgaga tgaccgcacc ctgtggggtg
     6481 ccttcctccg ttgctcgcca ttgaccctcc tccgacgtcc cggctgtgag ccacttacgt
     6541 atgagcctga ttaccctccg gtcgccgatc cgatgctctg tgaacctgat cagccattcg
     6601 tggctcaccc tgtcgaagaa ctgactgatg tcggcatcca gtacccagtt tacgttagtg
     6661 cgtaccagcc ctgtggccag tgcgtccagt gcatcgtgct ggcttcgccc gggtctgaac
     6721 ccgtatgaga accccataaa gtcgttttca tagactgcgt tcaggatttt caccagcgca
     6781 tactggacga tcttgtcctc cagcgaggcg atgccgagcg ggcgttgttt tccatccgct
     6841 tttgggatgt agtgacgcct gccgggctgc gccctgtagc tgccctgatg tagcctccgg
     6901 tgcagatctg ttatgttgtt cttcatgttt ccggcgtagt ccatccacct gatgccatcc
     6961 actccggcgg ccgctttcct gctcagggag aggaatgcgg cttccagtgc ttcgactgtc
     7021 agcaggtgga acaatgctgt aaaccgttct ttcttccgct gcttcgcagc ttcccgcacg
     7081 cgtgacagcc tctgtgacat gctttcccgg ctctgtgtcc ggcgcatgtg tggctgttcc
     7141 gcgttcccct tggccccgct ccttcgctcc actgactccg ctcctttcgg gttgttcgcc
     7201 tgcttcgccg ctactatgag cgagtccgac ttctcctctc cgtacatcac cggctatgac
     7261 tcctcgtctt cccggtgcgg gccatctccg acactggcag atggtcagag gggagatctc
     7321 ccggttcccg cgtagagatc gtattgacat gccagggtct cagaccccgc cgggtccatg
     7381 tggcactcgc agtatcgcac cctatgatgt tgccttccgt taacagtaca acgtcggcac
     7441 ccggtaattt aatatacatt tcgtggctca atggctggcc tgtcaacacc cctgtcaacg
     7501 cttcgcccca tacctcgcgg tatgcaacgc atgactcggg gaccttgtgg attgctggtc
     7561 cttcaatggt cggggacttt caccccttga tctctaaccg gtctcccggc gcacactgta
     7621 catatacaca gtataattat taccttactc ctcccgtttt caggtgtcaa tatatgagat
     7681 tagttctcat cttcaggcac aaaaaagcgc ccggagccac ctgtaagcag atcgcgtctt
     7741 ttcccgaaaa tttctcatgg caacaaattt tattatttaa gacattcatc acacttacat
     7801 ttcagatggt atagacacat ctgaaagcta actggcatga ggagaggacc tgcggttaaa
     7861 ctgttcggga aacctgagac gcccggaggc cactatgacg cacctgaaac tgactcccgc
     7921 tgaagaaaag cgcctggcgg acgcctttgt cgctcaggca ctggccatgg gcctgaaagg
     7981 gaacaaaacg ctgccaaagg cggtaaaaca acaggtgcgg gaaagggtgc ttcgtgccat
     8041 tcaggacgaa cgtgaagcca ggcgggcgca ggaaatgcgt cgggagaaat acgaggctac
     8101 ggaaaccacc ttcacctggc agccacagcg ccgccggtaa ggggtattta ccataacgtc
     8161 gcattgcccg caccgctcag acggtctgag ggcgtttccg gcggctacgg gataaaacag
     8221 gcaacaaacc acccggaaaa ccgccggaaa cgcgcccggt aaattgtaac ccgctgcatg
     8281 cctatgcacg caactgcatg gcgggccgtt tggtgtaaaa acgatacgat aagccgtgtt
     8341 tatgtaaatg aacgtggccg taaagagtga caaatataca tgtaacattt tttactgaca
     8401 ggtcatcaac ccgatgcgtt taataccctt tatcctgctg gcattgtgca gccccttact
     8461 cctgacggcc tgtaagcatt ccaccaccca gccgccgaaa atttatactc tggataagtc
     8521 atctaaaatc agtagctacg ctgcggccat caagaccgct gttgaaatgc agatgtttga
     8581 accagaggcc tatgcgggaa aaatgtgcac cgtgcggata tccatacaac gtgatggcac
     8641 tctgaacagc gccattgctg aaggaggaga tccgaaactc tgtaaagcag caatatcagc
     8701 cgttacccac gccaaaattc cgccggcacc ggatgaaaat acctggcaaa tattcaaaaa
     8761 cgcccctctg gatttcagac catgaaagct tttactctga cagtgctggc ggccatgttc
     8821 ctcactccgg tacatgcgat accactaaaa cccgtaacca cactgcagcc aacctacccg
     8881 gtacaggccg cagcaaaacg catcgaaggt caggtgaccg ccacgtttga tattctggac
     8941 gatggacgag tggacaacgt gtcgcttacc ggctgtcacc ttttttttcg ggaaacgctg
     9001 acggcaatga ggcactggct atatcccgca gggcacccgg caatgcgaca ggttataaat
     9061 tttgaattca gtctgaaaaa tgacggctta caggatccgc gaccagtcac cacccaaaag
     9121 ctcaatgatg atggttgccg ccagtaacag gtcctttaac ccgcaggctg caatagtcct
     9181 ggatgatgag tgagcctgta gcagccctga ttagtggaag cgaagctgaa aaatgggcag
     9241 caaaagccgt atttctggca aatagcgccg ccacggatta aagtagtcat gtgtaataca
     9301 tatgactaca aaggggcttt tatgtcatca gcaacatcca tcaggctcga tgaagaactt
     9361 aaggaccggc tgaaaacgct ggccgatgac cggcaccgct cagcgcacgc gctaatgctg
     9421 gaggcaatca ccgaatacat cgaccgggaa gagaagcgca gccagtacct gcgggacgga
     9481 caggccgcat ggcagcacta tcaggaaacg ggtctgcacc ttactgcgga agaagcggaa
     9541 gcctggataa gtacctgggg aactgagaac gagcaggatg ctccgccatg ccacaggtaa
     9601 cgatttccgc gctggcacag cgcgatttac aacgccttca ggactttctc aaaaccaaaa
     9661 atcggctggc agccagaaaa ggaggtgagg tgatcgtccg ggctatccag caactgaaaa
     9721 cattgccaga cattggccgc ccggtgccgt ttctgccgct ggaatataag gaactggtga
     9781 tcgggtttgg cgacagtggc tatgtgatgc tctaccgcca cgaccgggaa atggaccaga
     9841 ttgtgatcgt caccgtcagg catcagaaag aatccgggta tccgggggca gacagcctct
     9901 aataaccgat gacgggggcg caattcattg cgtggccgtc gccggtttga caagcgcagc
     9961 gcgtcagaaa accacatcct gaacggaaaa acgggtaaaa ttcagcgata catttaccgt
    10021 gagaaattac acacctttca cacccgataa aatcaggggt atacagtggc gaaaacattg
    10081 ctcaaccttt cacaggcagc acaagccgcg ggcattacca gaagaacgct atacaatcat
    10141 gtaaaacagg gaaaggttac cgtgtcacgt gacgggaaaa acaaccctgt cgtggatgtg
    10201 tcagaactga tccgtgtata cggaaatgtg aatatccctg agaaacaaat tcccggcatt
    10261 tcacaccgtg aaaatacaca aaaaaatttc ccacaggaac agcttctggc aatgcaaaag
    10321 gaactggccg atctccggca ggctgtcacc ctgatgctgg aagataaaac atcccgggaa
    10381 gaagaacgcc gccagcacga tgatgagcgc cggaaactcc aggccgaagt ggacagattg
    10441 acaacagaac tgacacagaa gaaaaaacgc ttctggtccg gctggtttag ctgagaactt
    10501 accgaaggga atggagaaca tgacgaagca ggaaaaaact gccctcaata tggctaaatt
    10561 cattaaggat cagtcgctgc tgttgctgga aaagctgaac gagctggaca tggatacccc
    10621 ggccgacatg tgcgaacggc ttcacgagga tgcggaaaca ctctggcagg tgatgcggga
    10681 taagctcggg gaagaataac agcaccggcc atgccggtgc tggcgttatt ttttgccgaa
    10741 cggccaccac cggcgggcgg gtttgtcctc cggcagttca ggcaccggca cgttaaaata
    10801 gcggctgtta tattcccgca tcagctccgc tttcagcttc tcgttcaccg cgctttcccc
    10861 gtgccgctcg ctgaccacct caatcatcgc gttaatctgc tccggtgagc acttcccggc
    10921 atcaaccacc tgcacaccgt tctcgatggc gaaccgctcc acctttcgat agaggtaatc
    10981 acacagcttc attcctgtgg gtttgtcccg ttcccattgc ggattttcac cggtgaaaaa
    11041 gtacgtggct ttttcgtcgc tgttgagccg ctcgtcatac atcccgatgg atttcatcat
    11101 cgccagatgc tgggcccggt cgacaaaaca gaaaacatac gccccggtct gccaggcgag
    11161 caccaccatg tcttccagat gctcgctggt tggcataatg cagcgctcgc cgtctttgtc
    11221 ttccacgtcc tcaataaata ccatgtacag cgccataatc cctcactccg ttgtcataaa
    11281 tttaaagcac gcgaacagcc tctcccggtc cagctccacc gttttgcggg cctcgccggt
    11341 ctgtggattc acgatttcat ccaccgccag aatcgcccgc tgtacccgga caagcgtcgg
    11401 ccggtcaggg cagatgtacc agatttcatc gatgccgttc gcccggcggt tgaacagata
    11461 cccggacacc acttcctgat accgcctgcg gctcttgacg gtgcgctcta cctcaaatgc
    11521 caccgttctg ccgtccagct ccgccagcgc gtccggcact ttcatccctt tcagggccat
    11581 acggtgcagg taccgccagc cggtggcccc ccggccctcc atggagaggc gcagggactg
    11641 cacggccagc cggtgcggca gctgcgccgc cgacaccctt gacggctgaa acggaataac
    11701 gtccgtcagc ggctcgtcct cgtcgaacga gaacgccacc ccgtgcggcg tcagccccca
    11761 cacgctgacc tgacggccgt caaccgggta tttcgcccgg accagcagcc ctttcgccac
    11821 cagactgtcc aggaagcggt aaaccggctg taccgccgca atccccagcc gtcgcgcaac
    11881 cagctccgcc gtggaacaca gctcttcccg cagccagcgc agggtcagcc gtgttttctc
    11941 cgcgttccgc gcctgccgct tcgcgtggct
//
