LOCUS       NZ_CP095252             5596 bp    DNA     circular CON 05-JUN-2024
DEFINITION  Klebsiella pneumoniae strain 21080534 plasmid p21080534_5, complete
            sequence.
ACCESSION   NZ_CP095252
VERSION     NZ_CP095252.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27363255
            Assembly: GCF_022982595.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   He,Z.
  TITLE     Comparative Genomic Analyses of Klebsiella pneumoniae in Anhui,
            China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   He,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2022) School of life science, University of
            Science and Technology of China, 1129 Huizhou Ave, Hefei, Anhui
            23005, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.4.9b; SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022982595.1-RS_2024_06_05
            Annotation Date                   :: 06/05/2024 03:17:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,799
            CDSs (total)                      :: 5,677
            Genes (coding)                    :: 5,504
            CDSs (with protein)               :: 5,504
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 66 of 173
            Pseudo Genes (incomplete)         :: 113 of 173
            Pseudo Genes (internal stop)      :: 20 of 173
            Pseudo Genes (multiple problems)  :: 25 of 173
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095252.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="21080534"
                     /isolation_source="Diversion fluid"
                     /host="Homo"
                     /db_xref="taxon:573"
                     /plasmid="p21080534_5"
                     /geo_loc_name="China: Anhui"
                     /lat_lon="31.85777 N 117.291451 E"
                     /collection_date="2021-08"
                     /collected_by="Zhien He"
     gene            490..714
                     /locus_tag="MU311_RS28835"
                     /old_locus_tag="MU311_28835"
     CDS             490..714
                     /locus_tag="MU311_RS28835"
                     /old_locus_tag="MU311_28835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            698..964
                     /locus_tag="MU311_RS28840"
                     /old_locus_tag="MU311_28840"
     CDS             698..964
                     /locus_tag="MU311_RS28840"
                     /old_locus_tag="MU311_28840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1034..1384)
                     /locus_tag="MU311_RS28845"
                     /old_locus_tag="MU311_28845"
     CDS             complement(1034..1384)
                     /locus_tag="MU311_RS28845"
                     /old_locus_tag="MU311_28845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1450..1995)
                     /locus_tag="MU311_RS28850"
                     /old_locus_tag="MU311_28850"
     CDS             complement(1450..1995)
                     /locus_tag="MU311_RS28850"
                     /old_locus_tag="MU311_28850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2021..2347)
                     /locus_tag="MU311_RS28855"
                     /old_locus_tag="MU311_28855"
     CDS             complement(2021..2347)
                     /locus_tag="MU311_RS28855"
                     /old_locus_tag="MU311_28855"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(2705..2863)
                     /locus_tag="MU311_RS29060"
     CDS             complement(2705..2863)
                     /locus_tag="MU311_RS29060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            3503..3679
                     /locus_tag="MU311_RS28860"
                     /old_locus_tag="MU311_28860"
     CDS             3503..3679
                     /locus_tag="MU311_RS28860"
                     /old_locus_tag="MU311_28860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3669..4013
                     /locus_tag="MU311_RS28865"
                     /old_locus_tag="MU311_28865"
     CDS             3669..4013
                     /locus_tag="MU311_RS28865"
                     /old_locus_tag="MU311_28865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4222..>4656
                     /locus_tag="MU311_RS28870"
                     /old_locus_tag="MU311_28870"
                     /pseudo=""
     CDS             4222..>4656
                     /locus_tag="MU311_RS28870"
                     /old_locus_tag="MU311_28870"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4691..5200
                     /locus_tag="MU311_RS28875"
                     /old_locus_tag="MU311_28875"
     CDS             4691..5200
                     /locus_tag="MU311_RS28875"
                     /old_locus_tag="MU311_28875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            5207..5419
                     /locus_tag="MU311_RS28880"
                     /old_locus_tag="MU311_28880"
     CDS             5207..5419
                     /locus_tag="MU311_RS28880"
                     /old_locus_tag="MU311_28880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          3338..3417
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(2755..3152)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          4004..5535
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggctgcatg gctatgcagc caggtaaaat ttatctgtcg cgaacggggc ggtttatcgg
       61 gtggtttgtt gccggtttta cctgactgcc accccgttcg cggcgaaccc gtccggggcg
      121 gtgcgggcaa cgggtgttat gtttaatcag cgtgaacggc agatacaaaa aagccccgga
      181 gggcttttaa ctactgactt atttttttga agatttgctg ggtttcaatt tgtcagtatt
      241 tcccggcgtt tttttattgt cacgctggcg tttatcaggt tcccctgttg aatcggcaat
      301 tcgtttcgga cccggcttaa gacccataat cgttatcctt tcaatagtta agaggaaagc
      361 cctcaagcta gatatattga ccgcgttacg gattatcaag aggggctaac aagattattt
      421 tttgttcttt taggttgatt gtggtactgt tgatatacat gtatagcaaa tggtgttcgg
      481 gaggtttata tgcttgctat ccggttatct gatgagattg agtcccgtct ggactcgctg
      541 gcgaagcaaa ccggcagaac aaagacgttt tatgcgcggg aagcaatact ggcgcatctg
      601 gaagacctgg aggattatta tctttcagca gaaactgctg cacgcgttcg ccgtggtgat
      661 gaagcagtgc attcgtctga agacgtgagg aagtcacttg gtctggacga ttaactattc
      721 cgatcgggcg ctcaaatcgt tacgcaagat ggacaaacag aacgcacgac ggattgtgga
      781 ttttatgagt ttacgcattg cagttgctgc cgatcctcgc cagtcaggga agccgctcaa
      841 aggtgagctg ggcgagttct ggcgctatcg ggtgggagat tatcgcgttc tgtgtgagat
      901 ccgagatgac gagcttgtta tccttgccgc cacgattgga catcgccgcg aagtttacga
      961 ctgaagcccg ctatgcgggc ttttttgatg ccgacaacag gtattatggt aaatactcga
     1021 ctaactacgt gttctactgt ttgtcggttt tagttaacca gcaggttgtc ttaacgataa
     1081 tgtagccgtt atttttaact aataaggtca tattgttaac gtcaggcata ataaaagaca
     1141 ttatggcctc agagttatca tcagaagcag ctctctgcat tactacttcg attccctttt
     1201 ctttgaatct ataagtatcc agttgcctat taaacttaga aagacctaca accatactta
     1261 gtttgttatc tttaacagta agagatgctt cctgagtacc attacatcgg tagtaagcgg
     1321 tgtaaccgtc agcaaaagat cgaccgggga atataaatgc taggaacgca attaaccaat
     1381 acatagttga gaatcttggt attacacatt tcactatcct ctctttcatt tttcgttcac
     1441 catactttgt tatctgattt ttaatttttc tattttataa accaagtctc taccaaaaag
     1501 gcgaaaatta tcctcaaata ataataattg tttaagaaca tccatgaaag cagctgcttc
     1561 accctcagtt atgggagcta ctctatataa catataacct gagttaaggg tttcttttat
     1621 actttcttca atgctcatta taaatattag tggattatca tttttcttcc cccatgggtt
     1681 tctaattaat ccttcgatgt actcaggtcc tttgcgtcct attgcctgcg caatattttt
     1741 tatttcatct gcgtgagggc tttgttgtac agctttgttt atgcctgctt gagtgatttt
     1801 cagagactct acaccgtgta tgttgtatga gataaaatgt aataaattaa gatgcaatgc
     1861 aattaatcca tttttgttta catctgagta tttggataaa aaacgtaatg aagtgttaac
     1921 tctcccttga agcctaacaa ttgggagtgc tgccttgtat atttccttct ccgttaaata
     1981 ccgtttaaaa aacataattc tccgcctccg tatagcgtgc ctattttatt tcgtcgtaaa
     2041 acttagctct gaaatattcc tttagaacca tcatgtcatt aataattgaa tctatctcat
     2101 ccattttttc ttggtattct tttaatggtg aagggagcgg taactcaata tttgaaaagt
     2161 gattggcgag atagggatca aggtttctca tccattcagg cttttcgcca atgcgttctt
     2221 caactgaaag tgaacctgat aatctctggt taataagatc acttagactc attttggttt
     2281 tattcgaaag ccgttgtaag gattgcttta atcctatatc tagcatgata tttgttcttt
     2341 ctctcattac ccccccttgc tattgcgttt tttgttgcgc acaaagctac cattgcgcat
     2401 taagaaacgc aataaaaaag gagggaggga gtggtttgta catgatgtgc agtgcgctga
     2461 gggcatcagt ggcgggtgtt tttccgtggg atggatggag ctccggggat aacagaactg
     2521 acgatcattt aaaggatctt cttgagatcc tttttttctg cgcgtaatct cttgcactgt
     2581 aaacgaaaaa accgcctggg gaggcggttt gatcgaaggt taagtcagtt ggggaactgc
     2641 ttaaccgggt aactgggtat acgagaccgc agataccaaa actgttcttt cagtgtagcc
     2701 gcagttaggc acccacttca agacctctca atatctctct cgtatatatc cagttaccaa
     2761 tggctgctgc cagtggcgtt tttgcgtgtc cgtccgggtt ggactcaaga tgatagttac
     2821 cggaaagggc gcagcagtcg ggctgaacgg ggggttcgtg catacagccc agcttggagc
     2881 gaacgaccta aaccgagccg agataccagc agcgtgagct atgagaaagc gccacgcttc
     2941 ccgaaaggga aaaaggcgga acaggtatcc ggtaaacggc agggtcggaa caggagagcg
     3001 cacgagggag ccgccggaga gaaatcaccg gcatctttaa gtcctgtcgg gtttcgtcct
     3061 cctctgactt gagcgtccat ttctgtgatg ctcgtcaggg gaggcggagc ctatggaaaa
     3121 acggcaacgc cgcatccttt tctccggccc tttctgattt gagcgccttt gctttatccc
     3181 gttatccgtg agtcggtcac gcccgccgca gccgaacggc aggagcgcag ctaccgagtg
     3241 agcgaggagg cgtcatttca tctgtccgtg catggtattg cggacgctcc tgagccacat
     3301 atcacacctg ccacatgagg cactttcatg aatcgtgctc tcatgccaac atagtaagcc
     3361 agtatacaca gcgtaaacgc tgtgactggt tcagggctgc gccccgaaac ccgctaacac
     3421 cactgacgcg ctggcgcttg tccaacgccg gagcgacccg gaaaactttt ccgtgccgtc
     3481 cggcgtttgc gaaggaggga taatgcagaa gccgaagaag aagccgctcg tcgtaaaaaa
     3541 aagatcctgg catccggcag tgatggcaaa tctgatgcgt caggcacagg aggaagatta
     3601 ccgccgggag cagatggcag agcttgagcg ttcagcccgg tcacattttg cggatatcgg
     3661 aggagaggat ggcggttaag cgtacccgtt ttttaggtat ccgggtgacc gatggggagt
     3721 accagcagct gctggagcgc tgcaatggaa ggcaactggc ggtgtggatg cgggaaacct
     3781 gtctcgatac gcgcccggcg cggtcgttgc ggctgccgtc catcgatccg gttttgctgc
     3841 gccagcttgc cgggatgggg aacaacctca accagatcgc ccggaaaatt aacggcggcc
     3901 agtggtcagg cgctgaccgg gttcaggtgg tggccgcgct gatggccatt gatgccgaac
     3961 tcgagcggct gcggcatgcc gtgctggaaa agggggcaga cgatgatcgt taagtttcat
     4021 ccgcgagggc gaggcggcgg tgccgggccg gtggattatc tgctggggaa agaccgccag
     4081 cgcgacgggg ccatcgtcct gcagggaaag ccggaggagg tccgggagct tatcgacgcc
     4141 tcgccctacg ccaaaaagta cacctccggc gtcctgtctt ttgctgaaca ggatttaccg
     4201 cccggccagc gtgaaaagct gatggcgagc ttcgagcggg ttctgatgcc cggactcgat
     4261 aaagaccagt acagcgtgct gtgggttgaa caccgggaca aggggcggct ggaactgaat
     4321 tttctggtac cgaacacgga actgctgacc ggcaggcgtc tccagccgta ctacgaccgg
     4381 gcagaccgtc cgcgcatcga tgcgtggcag accgtggtga acgggcgact ggggctgcac
     4441 gacccgaacg caccggagaa ccggcgcgcg ctggtgacgc catcttcgtt acccaaaacg
     4501 aagcaggaag ccgcagaggc tattacgcgg ggcttactgg ccctcgcctc gtcaggggag
     4561 cttaaaacgc gtcaggacgt cactgaggcg ctggaaagcg caggttttga ggtcgtgcgc
     4621 accacaaaga gcagcatcag cattgccgac ccggacggag ggcgaaacat ccgacttaag
     4681 ggagccatct atgaacagtc ttttaacgct ggcgaaggac ttagagcaga aatcgaaagc
     4741 gcagcagcag agtaccggcg aaatgctgaa agccgcattc agcgagcacg agaagtctgt
     4801 cagagcggaa ctgagcgaaa gcgggaagag aatcagcgcc gccatccgcg cccacgagca
     4861 gggtatgagc gcggccatgc agtcgaaccg cctgagcgtg atgcgtatgg tcgggcggac
     4921 atggctgacc atcacgatgg tgtcaggact gctgttcgcc agcctgagcg gggtgttgtg
     4981 gtatcagggg agcttgatag cgtcaaatct ggccgaaatc gaccggcaga acgcagcgct
     5041 gtcgaagctg aacgcgaaga cctggggcgt gacgtacctg gaagacagca acggacgttt
     5101 tctggtgctg ccgaagggga cggccgtaga caggacgcag agctggacgg tcgggaacgg
     5161 gcggtcgaag cagaacgcgc tgagactggt gaaggagtaa gtgccgatga ccgagctgga
     5221 aaaacagttg ctgagcgcat tagagcagct acagcaggac tactcgaaaa ggctggacga
     5281 gtgggagagc gccttcgggg aatggcggaa aatgtcaggg cttatgcaac gggagaacgc
     5341 ggcgctgagc gagcgcgtga cgcgcttgag tcagcaggtg gaacgcttga gcgggcaact
     5401 gcagcgtttg agccggtagt gcagcgtcat gagatggccg tggccgcaga acgggcgcac
     5461 gagcaacggc agcacgaaaa ggaactgacg gaagcgcgtt cccggcagcg aagctatgac
     5521 ggtccgtcgc tgggctgaaa tgcaggaaaa cagggttctc atcgggcatt tacgctgaaa
     5581 ccggatgtca ttcacc
//
