LOCUS       NZ_CP095246            10060 bp    DNA     circular CON 05-JUN-2024
DEFINITION  Klebsiella pneumoniae strain 21080237 plasmid p21080237_6, complete
            sequence.
ACCESSION   NZ_CP095246
VERSION     NZ_CP095246.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27363254
            Assembly: GCF_022982555.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   He,Z.
  TITLE     Comparative Genomic Analyses of Klebsiella pneumoniae in Anhui,
            China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   He,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2022) School of life science, University of
            Science and Technology of China, 1129 Huizhou Ave, Hefei, Anhui
            23005, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.4.9b; SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022982555.1-RS_2024_06_05
            Annotation Date                   :: 06/05/2024 03:31:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,923
            CDSs (total)                      :: 5,800
            Genes (coding)                    :: 5,625
            CDSs (with protein)               :: 5,625
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 68 of 175
            Pseudo Genes (incomplete)         :: 116 of 175
            Pseudo Genes (internal stop)      :: 24 of 175
            Pseudo Genes (multiple problems)  :: 33 of 175
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095246.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="21080237"
                     /isolation_source="Diversion fluid"
                     /host="Homo"
                     /db_xref="taxon:573"
                     /plasmid="p21080237_6"
                     /geo_loc_name="China: Anhui"
                     /lat_lon="31.85777 N 117.291450 E"
                     /collection_date="2021-08"
                     /collected_by="Zhien He"
     gene            complement(join(9196..10060,1..1100))
                     /locus_tag="MU300_RS29435"
                     /old_locus_tag="MU300_29430"
     CDS             complement(join(9196..10060,1..1100))
                     /locus_tag="MU300_RS29435"
                     /old_locus_tag="MU300_29430"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(2584..2733)
                     /locus_tag="MU300_RS29440"
                     /old_locus_tag="MU300_29435"
     CDS             complement(2584..2733)
                     /locus_tag="MU300_RS29440"
                     /old_locus_tag="MU300_29435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(2818..3075)
                     /locus_tag="MU300_RS29445"
                     /old_locus_tag="MU300_29440"
     CDS             complement(2818..3075)
                     /locus_tag="MU300_RS29445"
                     /old_locus_tag="MU300_29440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3085..4770)
                     /locus_tag="MU300_RS29450"
                     /old_locus_tag="MU300_29445"
     CDS             complement(3085..4770)
                     /locus_tag="MU300_RS29450"
                     /old_locus_tag="MU300_29445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5097..5342
                     /locus_tag="MU300_RS29455"
                     /old_locus_tag="MU300_29450"
     CDS             5097..5342
                     /locus_tag="MU300_RS29455"
                     /old_locus_tag="MU300_29450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            5616..5987
                     /gene="tolA"
                     /locus_tag="MU300_RS29460"
                     /old_locus_tag="MU300_29455"
     CDS             5616..5987
                     /gene="tolA"
                     /locus_tag="MU300_RS29460"
                     /old_locus_tag="MU300_29455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            5984..6349
                     /locus_tag="MU300_RS29465"
                     /old_locus_tag="MU300_29460"
     CDS             5984..6349
                     /locus_tag="MU300_RS29465"
                     /old_locus_tag="MU300_29460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            6524..6802
                     /locus_tag="MU300_RS29470"
                     /old_locus_tag="MU300_29465"
     CDS             6524..6802
                     /locus_tag="MU300_RS29470"
                     /old_locus_tag="MU300_29465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            6790..7104
                     /locus_tag="MU300_RS29475"
                     /old_locus_tag="MU300_29470"
     CDS             6790..7104
                     /locus_tag="MU300_RS29475"
                     /old_locus_tag="MU300_29470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            7268..7696
                     /locus_tag="MU300_RS29480"
                     /old_locus_tag="MU300_29475"
     CDS             7268..7696
                     /locus_tag="MU300_RS29480"
                     /old_locus_tag="MU300_29475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            7722..7901
                     /locus_tag="MU300_RS29485"
                     /old_locus_tag="MU300_29480"
     CDS             7722..7901
                     /locus_tag="MU300_RS29485"
                     /old_locus_tag="MU300_29480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7928..8458)
                     /locus_tag="MU300_RS29490"
                     /old_locus_tag="MU300_29485"
     CDS             complement(7928..8458)
                     /locus_tag="MU300_RS29490"
                     /old_locus_tag="MU300_29485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_137970705.1"
                     /translation="MALYMVFIEDVEDKDGEHCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8465..9196)
                     /gene="mobC"
                     /locus_tag="MU300_RS29495"
                     /old_locus_tag="MU300_29490"
     CDS             complement(8465..9196)
                     /gene="mobC"
                     /locus_tag="MU300_RS29495"
                     /old_locus_tag="MU300_29490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     BGC             1..9770
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1903..2481
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8469..9196)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 gcaatcagct tcgccgcttt ctgctcgctg atgcggtagt ggtccgccac gtcgccggtc
       61 tcactcaggt taaacccggc aaccagcaga ttcttcaggg cgtactggtc gagatcgcgg
      121 aacagattca gctgcgccgg tttccccggc cgcagatcca gcagggtgaa gtccgggcag
      181 tgcgcccgga agacgtgcgg cgcccacgcg tcccctttgg ggtcgaagta caccacagcg
      241 tccccgaaat cgcgtacgca gcgaatgagc gcgttggtgg ccatcgcgct cttgccgctc
      301 ccgggcaggc cgaggatctg aatattggtc ttgcgccagg tatcacgagg gaccagcacc
      361 ggctggccct gctcatcctt acccagaaac accgcctgct gtttccgggc ctgctccgtg
      421 gaaaaatcgg ccagcgcgtt aaccggtacc ggctccccga aatgcaggtg gcggacgtct
      481 gtcatttctc cggtccgctg gctgctgcgc cggctctcct gattcagttt cgcgttcacc
      541 gcctcgtcag cggccgcaaa atgaccatca agcagcagga acgccagcag ccgcgccaga
      601 aggacgccgg cgacaagccc tccggcacac caggctatcc cgaacaggtt agccggctgc
      661 agcaggtaat aatctgcgtt cgcatagagg tccgggcgca ggacggtttc tccccagctc
      721 tcgataaaca cggcgacaac cagccccccc agaagggcca gaccggcagc gccggtcagc
      781 cagtacgcgt agttccagac atgtcgccgt gccctgacca tccgcccggg tgaatccagc
      841 gaggccagca ccgaaaatgg cacagtccgg ttattatcgc gcttatagcg atcggcatac
      901 tcccgccgcg ccttatggct cagccccaca aaacgcattc ggccggccca gagttcagcc
      961 agaccgaaaa acaggccgat aaccagaaaa aaaccggcca gactgtaata ctcccggtac
     1021 agccagctga cccacgggta aatctgccgg gccagctccg ggatatgcca ctgccaggca
     1081 agcgaatccg tattaaacat gcaatctctc tccttcagac gacgggttta ccggcgtgat
     1141 gcgcagcccc cgctgcacaa gccgcggaca tgccccaagg catggcggcg gcggtcacgt
     1201 ccgtggtgat tgcgttcagc tttcaccgcg gaggccagcc tctttccgcc ccatgcggaa
     1261 aaggcgcagc cccggaggaa caacgagcac gccccagcgt gtgagaggaa aaggcggcac
     1321 cccagtgcgg tcttttccgg tgttccggcc cccgtcagag ttcgccatag cgagcggctg
     1381 accacccgca aaatcaggag cagctttgct gcttcgtcag gtgcgtccag caccgtggat
     1441 ttttgtgggt ggtcaggcgt gagcctgacg ggggccgggt gaatttctgg cgtggccggc
     1501 gtccagccgg acagagccag aaagagggga aaagaccgtg cgtcggtggc gtccagccag
     1561 cgtaagcaag gtcttttccc ctgaagtcag ccacgcccca gcggggatga cttgcatttc
     1621 tccgcaagga gaaaaaagca acagagatag gttaacgtaa aatgttcagc gaaaaaccga
     1681 catggttcac ctatcctgat aattgatcgt cagggaatag aaagacttaa tcaggggaca
     1741 atatcccaca tcagcgcgtc cggtgaccgt cttgcaggtg ccggggaaat tgcgcctcct
     1801 cgctcactcg gtcgctacgc tccgggcgtg agactgcggc gggcgctgcg gacacataca
     1861 aagttaccca cagattccgt ggataagcag gggactaaca tgtgaggcaa aacggcaggg
     1921 ccgcgccggt ggcgtttttc cataggctcc gccctcctgc cagagttcac ataaacagac
     1981 gctttttcgg tgcatctgtg ggagccgtga ggctcaacca tgaatctgac agtacgggcg
     2041 aaacccgaca ggacttaaag atccccaccg tttccggtag gtcgctccct cttgcgctct
     2101 cctgttccga ccctgccgtt taccggatac ctgtccgccg ttctcccttg cgggaagtgt
     2161 ggcgctttct catagctcac acactggtat ctcggctcgg tgtaggtcgt tcgctccaag
     2221 ctgggctgta agcaagaact ccccgttcag cccgactgct gcgccttatc cggtaactgt
     2281 tcacttgagt ccaacccggg aaagcacgca aaacgccact ggcagcagcc attggtaact
     2341 gggagttcgc agaggatttg tttagctaaa cacgcggttg ctcttgaagt gtgcgccaaa
     2401 gtccggctac actggaagga cagatttggt tgctgtgctc tgcgaaagcc agttaccacg
     2461 gttaagcagt tccccaactg acttaacctt cgatcaaacc acctccccag gtggtttttt
     2521 cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2581 tttctactga accgcgatcc ccgtcagttc agaggaggac gatggtgcca ccgttccgcc
     2641 ctgaacatcc cggatataat ttgcctgaca tgcggccaga agaaagccag caacaatcaa
     2701 tataaaaaga aaaatagtct ttaccttttt catatacttt ccctgtattt aaagactccg
     2761 gcattcatcc ctgaacaccg gaagtcagta tttcaaatca tccttgataa ttttttgtta
     2821 ccagttaccg tcccgataat caaaagctat ataacaatta tccacatcag gttttatttg
     2881 atttttaaaa aatggctgta gtaaaggaac ccattctttc ttcaaatcaa actcaccatt
     2941 attaatatta tcctttaaag gcattcccaa gctttcaatg acagaaccat cgtcaccaaa
     3001 gtctttagaa tactcttccc ctttaaactc ttcagttttc ttatcaaacc atgtcaaacg
     3061 taatttaagc cccatattta cctctcaaag atatttttta atgttccgtt ttggatctgg
     3121 ccctttaacc tgcttacccg ttttgggatc aaatgcgcct aggtgttcgc catcactggc
     3181 acgataccct tcaagctcac cgtgctggga atcccattca taaattttac gttttttatc
     3241 gccataccat cgggcccgct taccaccacc accttgttta ggtgttttag gatcacctcg
     3301 ctttaactct ccaagtccct ttatgtcttc tgttttaggt actggatgat aatcatggcc
     3361 gtagtcttta gtccccttgc gaggcttgtt cttttcctca tttaacttat tttctgcatc
     3421 cttcgctttt ttctcctttt gtttccggct ctccatcgcc ccggtaagtg cagccttatc
     3481 tctggataag gattcattca ttgcgtttaa agatgcctga gccgctttta atttctcatt
     3541 ctgattctga atatccttat caagatagcg aacctgatcc tgccacccgg agccatattc
     3601 atgaggcggg ttataagtca caaaatcatc tttatttgcc tctgcctctt ttaccttttt
     3661 atcggcttcc tgaacagcag cccttgcacc ggggagcgcc ttctctgtcg atgcaatgcg
     3721 gctgtttaag gtcgcaatat ttttatcttc ggcatccagc tcagctttcg ctttatcata
     3781 gtctcttttc agcccctctt ccggatgagc ggcgtcccat gcctgctggc gacgcttttc
     3841 ttcctcctga cgctgtttta cctgtgccgg ggtaagaaca tctgtcaccg acacataaac
     3901 cggcttctgt ccggtctctt tcgggaaacg aataacggcc tcacgggagt taccaccggc
     3961 tgtaaaacca gccggacgtg aatcaccttt ttcagcaaca atgccttttg gcggggcttt
     4021 cgctgtcgga acacctttcg gtacgctcac ctgcagagac gggagacccg gaatcgacac
     4081 actgaatacc cccggacgtt ttgtcggttt cgcatccacc acagggacac tcattggccg
     4141 acctgcgaca accgcaatat gctgccgctc atccttcacc acatccacaa cccgttgtct
     4201 gacacgaacc gtcgcctggt ccagtggtaa agtacttgcc ggagtctccg tcaccgcatc
     4261 ggccggtaat gacgtcataa tttttgacat cattttcgga tcatcttttg caatctcaga
     4321 aggcagcaca ccgtagatcg caatccccca cagaccaaac ttaaacggcc ccttcagggc
     4381 agccagcaca tcagcaacgg cggccgacaa cgcatcgccg gaaactgata aagccagtcc
     4441 ttcagccccc ggagtagcca gggcaggcag accgaaggcc atcgcagtgg ccgaagactg
     4501 tccgccacca gagctaccgc tatgattgcc gctgttacca ccattaccac cgggtttact
     4561 cccgccgtta ccaaactccc ccggattata gttatgaata tctccattcg gcgtatgcgt
     4621 cgtaccccag cccggcccgc tgtttggatc cgtaccacca cctgatgaag ggccaccttt
     4681 accagacgta ccgttcacat tcccactggc ctgaccacca ttatgtccca gacctgaatt
     4741 acccggacct cgaccgtctc caccactcat accgttttcc tctttgacat ttaaaacaat
     4801 aaattaaagc caacatactg tacatataca cagtataatt attaccttac tcctcccgtt
     4861 ttcaggtgtc aatatatgag attagttctc atcttcaggc acaaaaaagc gcccggagcc
     4921 acctgtaagc agatcgcgtc ttttcccgaa aatttctcat ggcaacaaat tttattattt
     4981 aagacattca tcacacttac atttcagatg gtatagacac atctgaaagc taactggcat
     5041 gaggagagga cctgcggtta aactgttcgg gaaacctgag acgcccggag gccactatga
     5101 cgcacctgaa actgactccc gctgaagaaa agcgcctggc ggacgccttt gtcgctcagg
     5161 cactggccat gggcctgaaa gggaacaaaa cgctgccaaa ggcggtaaaa caacaggtgc
     5221 gggaaagggt gcttcgtgcc attcaggacg aacgtgaagc caggcgggcg caggaaatgc
     5281 gtcgggagaa atacgaggct acggaaacca ccttcacctg gcagccacag cgccgccggt
     5341 aaggggtatt taccataacg tcgcattgcc cgcaccgctc agacggtctg agggcgtttc
     5401 cggcggctac gggataaaac aggcaacaaa ccacccggaa aaccgccgga aacgcgcccg
     5461 gtaaattgta acccgctgca tgcctatgca cgcaactgca tggcgggccg tttggtgtaa
     5521 aaacgatacg ataagccgtg tttatgtaaa tgaacgtggc cgtaaagagt gacaaatata
     5581 catgtaacat tttttactga caggtcatca acccgatgcg tttaataccc tttatcctgc
     5641 tggcattgtg cagcccctta ctcctgacgg cctgtaagca ttccaccacc cagccgccga
     5701 aaatttatac tctggataag tcatctaaaa tcagtagcta cgctgcggcc atcaagaccg
     5761 ctgttgaaat gcagatgttt gaaccagagg cctatgcggg aaaaatgtgc accgtgcgga
     5821 tatccataca acgtgatggc actctgaaca gcgccattgc tgaaggagga gatccgaaac
     5881 tctgtaaagc agcaatatca gccgttaccc acgccaaaat tccgccggca ccggatgaaa
     5941 atacctggca aatattcaaa aacgcccctc tggatttcag accatgaaag cttttactct
     6001 gacagtgctg gcggccatgt tcctcactcc ggtacatgcg ataccactaa aacccgtaac
     6061 cacactgcag ccaacctacc cggtacaggc cgcagcaaaa cgcatcgaag gtcaggtgac
     6121 cgccacgttt gatattctgg acgatggacg agtggacaac gtgtcgctta ccggctgtca
     6181 cctttttttt cgggaaacgc tgacggcaat gaggcactgg ctatatcccg cagggcaccc
     6241 ggcaatgcga caggttataa attttgaatt cagtctgaaa aatgacggct tacaggatcc
     6301 gcgaccagtc accacccaaa agctcaatga tgatggttgc cgccagtaac aggtccttta
     6361 acccgcaggc tgcaatagtc ctggatgatg agtgagcctg tagcagccct gattagtgga
     6421 agcgaagctg aaaaatgggc agcaaaagcc gtatttctgg caaatagcgc cgccacggat
     6481 taaagtagtc atgtgtaata catatgacta caaaggggct tttatgtcat cagcaacatc
     6541 catcaggctc gatgaagaac ttaaggaccg gctgaaaacg ctggccgatg accggcaccg
     6601 ctcagcgcac gcgctaatgc tggaggcaat caccgaatac atcgaccggg aagagaagcg
     6661 cagccagtac ctgcgggacg gacaggccgc atggcagcac tatcaggaaa cgggtctgca
     6721 ccttactgcg gaagaagcgg aagcctggat aagtacctgg ggaactgaga acgagcagga
     6781 tgctccgcca tgccacaggt aacgatttcc gcgctggcac agcgcgattt acaacgcctt
     6841 caggactttc tcaaaaccaa aaatcggctg gcagccagaa aaggaggtga ggtgatcgtc
     6901 cgggctatcc agcaactgaa aacattgcca gacattggcc gcccggtgcc gtttctgccg
     6961 ctggaatata aggaactggt gatcgggttt ggcgacagtg gctatgtgat gctctaccgc
     7021 cacgaccggg aaatggacca gattgtgatc gtcaccgtca ggcatcagaa agaatccggg
     7081 tatccggggg cagacagcct ctaataaccg atgacggggg cgcaattcat tgcgtggccg
     7141 tcgccggttt gacaagcgca gcgcgtcaga aaaccacatc ctgaacggaa aaacgggtaa
     7201 aattcagcga tacatttacc gtgagaaatt acacaccttt cacacccgat aaaatcaggg
     7261 gtatacagtg gcgaaaacat tgctcaacct ttcacaggca gcacaagccg cgggcattac
     7321 cagaagaacg ctatacaatc atgtaaaaca gggaaaggtt accgtgtcac gtgacgggaa
     7381 aaacaaccct gtcgtggatg tgtcagaact gatccgtgta tacggaaatg tgaatatccc
     7441 tgagaaacaa attcccggca tttcacaccg tgaaaataca caaaaaaatt tcccacagga
     7501 acagcttctg gcaatgcaaa aggaactggc cgatctccgg caggctgtca ccctgatgct
     7561 ggaagataaa acatcccggg aagaagaacg ccgccagcac gatgatgagc gccggaaact
     7621 ccaggccgaa gtggacagat tgacaacaga actgacacag aagaaaaaac gcttctggtc
     7681 cggctggttt agctgagaac ttaccgaagg gaatggagaa catgacgaag caggaaaaaa
     7741 ctgccctcaa tatggctaaa ttcattaagg atcagtcgct gctgttgctg gaaaagctga
     7801 acgagctgga catggatacc ccggccgaca tgtgcgaacg gcttcacgag gatgcggaaa
     7861 cactctggca ggtgatgcgg gataagctcg gggaagaata acagcaccgg ccatgccggt
     7921 gctggcgtta ttttttgccg aacggccacc accggcgggc gggtttgtcc tccggcagtt
     7981 caggcaccgg cacgttaaaa tagcggctgt tatattcccg catcagctcc gctttcagct
     8041 tctcgttcac cgcgctttcc ccgtgccgct cgctgaccac ctcaatcatc gcgttaatct
     8101 gctccggtga gcacttcccg gcatcaacca cctgcacacc gttctcgatg gcgaaccgct
     8161 ccacctttcg atagaggtaa tcacacagct tcattcctgt gggtttgtcc cgttcccatt
     8221 gcggattttc accggtgaaa aagtacgtgg ctttttcgtc gctgttgagc cgctcgtcat
     8281 acatcccgat ggatttcatc atcgccagat gctgggcccg gtcgacaaaa cagaaaacat
     8341 acgccccggt ctgccaggcg agcaccacca tgtcttccag atgctcgctg gttggcataa
     8401 tgcagtgctc gccgtctttg tcttccacgt cctcaataaa taccatgtac agcgccataa
     8461 tccctcactc cgttgtcata aatttaaagc acgcgaacag cctctcccgg tccagctcca
     8521 ccgttttgcg ggcctcgccg gtctgtggat tcacgatttc atccaccgcc agaatcgccc
     8581 gctgtacccg gacaagcgtc ggccggtcag ggcagatgta ccagatttca tcgatgccgt
     8641 tcgcccggcg gttgaacaga tacccggaca ccacttcctg ataccgcctg cggctcttga
     8701 cggtgcgctc tacctcaaat gccaccgttc tgccgtccag ctccgccagc gcgtccggca
     8761 ctttcatccc tttcagggcc atacggtgca ggtaccgcca gccggtggcc ccccggccct
     8821 ccatggagag gcgcagggac tgcacggcca gccggtgcgg cagctgcgcc gccgacaccc
     8881 ttgacggctg aaacggaata acgtccgtca gcggctcgtc ctcgtcgaac gagaacgcca
     8941 ccccgtgcgg cgtcagcccc cacacgctga cctgacggcc gtcaaccggg tatttcgccc
     9001 ggaccagcag ccctttcgcc accagactgt ccaggaagcg gtaaaccggc tgtaccgccg
     9061 caatccccag ccgtcgcgca accagctccg ccgtggaaca cagctcttcc cgcagccagc
     9121 gcagggtcag ccgtgttttc tccgcgttcc gcgcctgccg cttcgcgtgg ctgacgttgt
     9181 actgctccag tgtcatcagt aaagccccgc aaaatcatca tccgcttttg gtccgccgcc
     9241 gtcggccgga ggcaggtact ccgccagcgg gtccgttttc gccagcaccg ggaaggtttt
     9301 cagcgaaatt tcccggcgct caacccgcag cgggctgctg aacgccagcc gcgccacgcc
     9361 cagcccggtc atcaccgcga acccgtcagg caggtgttgc agggtgttca cgtcaaacag
     9421 cggccgcgcc tccttctgga tgaagctgtc gccgctgatg tgctccacgt tccccgcctc
     9481 ccggctggcc cggcggcgct ccacgtccac ccggatctcc ccggtctggc ccgccgccca
     9541 ctgcgcggac tcctggctgg ccgcgcgata gaaccagcgg atcggcgtgt tatccagtac
     9601 cgtggttttc acaaaatcag cgggcagatc ctgcccgcac tgaccgaagt cgcccagcga
     9661 ctggtgcgcg agcagcaggt tgcagtcccg gctgcgcagc gtccccagcg cgttcagcac
     9721 gtacttcgac agcagatatt taatctcatc gagcatgatg ctcgcgtgcg gccacctccg
     9781 gaactcatcc cgcgcaatga tgatttgcgc gcagcgggca aacagcatct tctgcacccg
     9841 gatcaccgcc tcatcgtcca tcgagccgat gacgtacagg cagccgccgc cgttgattat
     9901 ccccgccaca tcgataccgc tgtccgtctg caggacgctc aggtcggcca cgttctccag
     9961 cttggtgatc agccccttca cgtccttttt cagggcctcc ggcaacgcgt acgccgccgc
    10021 cagcacctgc tgaatgtttg ccccctgcgg gaactgctcc
//
