LOCUS       NZ_CP095239             5596 bp    DNA     circular CON 05-JUN-2024
DEFINITION  Klebsiella pneumoniae strain 21072329 plasmid p21072329_5, complete
            sequence.
ACCESSION   NZ_CP095239
VERSION     NZ_CP095239.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27363253
            Assembly: GCF_022982715.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   He,Z.
  TITLE     Comparative Genomic Analyses of Klebsiella pneumoniae in Anhui,
            China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   He,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2022) School of life science, University of
            Science and Technology of China, 1129 Huizhou Ave, Hefei, Anhui
            23005, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.4.9b; SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022982715.1-RS_2024_06_05
            Annotation Date                   :: 06/05/2024 03:13:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,790
            CDSs (total)                      :: 5,667
            Genes (coding)                    :: 5,520
            CDSs (with protein)               :: 5,520
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 147
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 0 of 147
            Pseudo Genes (frameshifted)       :: 47 of 147
            Pseudo Genes (incomplete)         :: 102 of 147
            Pseudo Genes (internal stop)      :: 17 of 147
            Pseudo Genes (multiple problems)  :: 18 of 147
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095239.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="21072329"
                     /isolation_source="sputum"
                     /host="Homo"
                     /db_xref="taxon:573"
                     /plasmid="p21072329_5"
                     /geo_loc_name="China: Anhui"
                     /lat_lon="31.85777 N 117.291449 E"
                     /collection_date="2021-07"
                     /collected_by="Zhien He"
     gene            629..787
                     /locus_tag="MU289_RS28985"
     CDS             629..787
                     /locus_tag="MU289_RS28985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1145..1471
                     /locus_tag="MU289_RS28810"
                     /old_locus_tag="MU289_28810"
     CDS             1145..1471
                     /locus_tag="MU289_RS28810"
                     /old_locus_tag="MU289_28810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            1497..2042
                     /locus_tag="MU289_RS28815"
                     /old_locus_tag="MU289_28815"
     CDS             1497..2042
                     /locus_tag="MU289_RS28815"
                     /old_locus_tag="MU289_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            2108..2458
                     /locus_tag="MU289_RS28820"
                     /old_locus_tag="MU289_28820"
     CDS             2108..2458
                     /locus_tag="MU289_RS28820"
                     /old_locus_tag="MU289_28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2528..2794)
                     /locus_tag="MU289_RS28825"
                     /old_locus_tag="MU289_28825"
     CDS             complement(2528..2794)
                     /locus_tag="MU289_RS28825"
                     /old_locus_tag="MU289_28825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2778..3002)
                     /locus_tag="MU289_RS28830"
                     /old_locus_tag="MU289_28830"
     CDS             complement(2778..3002)
                     /locus_tag="MU289_RS28830"
                     /old_locus_tag="MU289_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(3669..3881)
                     /locus_tag="MU289_RS28835"
                     /old_locus_tag="MU289_28835"
     CDS             complement(3669..3881)
                     /locus_tag="MU289_RS28835"
                     /old_locus_tag="MU289_28835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3888..4397)
                     /locus_tag="MU289_RS28840"
                     /old_locus_tag="MU289_28840"
     CDS             complement(3888..4397)
                     /locus_tag="MU289_RS28840"
                     /old_locus_tag="MU289_28840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4432..5136)
                     /locus_tag="MU289_RS28990"
                     /pseudo=""
     CDS             complement(<4432..5136)
                     /locus_tag="MU289_RS28990"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5075..5419)
                     /locus_tag="MU289_RS28850"
                     /old_locus_tag="MU289_28850"
     CDS             complement(5075..5419)
                     /locus_tag="MU289_RS28850"
                     /old_locus_tag="MU289_28850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5409..5585)
                     /locus_tag="MU289_RS28855"
                     /old_locus_tag="MU289_28855"
     CDS             complement(5409..5585)
                     /locus_tag="MU289_RS28855"
                     /old_locus_tag="MU289_28855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     Typing          complement(76..152)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          341..738
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(3554..5085)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgcaaacgcc ggacggcacg gaaaagtttt ccgggtcgct ccggcgttgg acaagcgcca
       61 gcgcgtcagt ggtgttagcg ggtttcgggg cgcagccctg aaccagtcac agcgtttacg
      121 ctgtgtatac tggcttacta tgttggcatg agagcacgat tcatgaaagt gcctcatgtg
      181 gcaggtgtga tatgtggctc aggagcgtcc gcaataccat gcacggacag atgaaatgac
      241 gcctcctcgc tcactcggta gctgcgctcc tgccgttcgg ctgcggcggg cgtgaccgac
      301 tcacggataa cgggataaag caaaggcgct caaatcagaa agggccggag aaaaggatgc
      361 ggcgttgccg tttttccata ggctccgcct cccctgacga gcatcacaga aatggacgct
      421 caagtcagag gaggacgaaa cccgacagga cttaaagatg ccggtgattt ctctccggcg
      481 gctccctcgt gcgctctcct gttccgaccc tgccgtttac cggatacctg ttccgccttt
      541 ttccctttcg ggaagcgtgg cgctttctca tagctcacgc tgctggtatc tcggctcggt
      601 ttaggtcgtt cgctccaagc tgggctgtat gcacgaaccc cccgttcagc ccgactgctg
      661 cgccctttcc ggtaactatc atcttgagtc caacccggac ggacacgcaa aaacgccact
      721 ggcagcagcc attggtaact ggatatatac gagagagata ttgagaggtc ttgaagtggg
      781 tgcctaactg cggctacact gaaagaacag ttttggtatc tgcggtctcg tatacccagt
      841 tacccggtta agcagttccc caactgactt aaccttcgat caaaccgcct ccccaggcgg
      901 ttttttcgtt tacagtgcaa gagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      961 taaatgatcg tcagttctgt tatccccgga gctccatcca tcccacggaa aaacacccgc
     1021 cactgatgcc ctcagcgcac tgcacatcat gtacaaacca ctccctccct ccttttttat
     1081 tgcgtttctt aatgcgcaat ggtagctttg tgcgcaacaa aaaacgcaat agcaaggggg
     1141 ggtaatgaga gaaagaacaa atatcatgct agatatagga ttaaagcaat ccttacaacg
     1201 gctttcgaat aaaaccaaaa tgagtctaag tgatcttatt aaccagagat tatcaggttc
     1261 actttcagtt gaagaacgca ttggcgaaaa gcctgaatgg atgagaaacc ttgatcccta
     1321 tctcgccaat cacttttcaa atattgagtt accgctccct tcaccattaa aagaatacca
     1381 agaaaaaatg gatgagatag attcaattat taatgacatg atggttctaa aggaatattt
     1441 cagagctaag ttttacgacg aaataaaata ggcacgctat acggaggcgg agaattatgt
     1501 tttttaaacg gtatttaacg gagaaggaaa tatacaaggc agcactccca attgttaggc
     1561 ttcaagggag agttaacact tcattacgtt ttttatccaa atactcagat gtaaacaaaa
     1621 atggattaat tgcattgcat cttaatttat tacattttat ctcatacaac atacacggtg
     1681 tagagtctct gaaaatcact caagcaggca taaacaaagc tgtacaacaa agccctcacg
     1741 cagatgaaat aaaaaatatt gcgcaggcaa taggacgcaa aggacctgag tacatcgaag
     1801 gattaattag aaacccatgg gggaagaaaa atgataatcc actaatattt ataatgagca
     1861 ttgaagaaag tataaaagaa acccttaact caggttatat gttatataga gtagctccca
     1921 taactgaggg tgaagcagct gctttcatgg atgttcttaa acaattatta ttatttgagg
     1981 ataattttcg cctttttggt agagacttgg tttataaaat agaaaaatta aaaatcagat
     2041 aacaaagtat ggtgaacgaa aaatgaaaga gaggatagtg aaatgtgtaa taccaagatt
     2101 ctcaactatg tattggttaa ttgcgttcct agcatttata ttccccggtc gatcttttgc
     2161 tgacggttac accgcttact accgatgtaa tggtactcag gaagcatctc ttactgttaa
     2221 agataacaaa ctaagtatgg ttgtaggtct ttctaagttt aataggcaac tggatactta
     2281 tagattcaaa gaaaagggaa tcgaagtagt aatgcagaga gctgcttctg atgataactc
     2341 tgaggccata atgtctttta ttatgcctga cgttaacaat atgaccttat tagttaaaaa
     2401 taacggctac attatcgtta agacaacctg ctggttaact aaaaccgaca aacagtagaa
     2461 cacgtagtta gtcgagtatt taccataata cctgttgtcg gcatcaaaaa agcccgcata
     2521 gcgggcttca gtcgtaaact tcgcggcgat gtccaatcgt ggcggcaagg ataacaagct
     2581 cgtcatctcg gatctcacac agaacgcgat aatctcccac ccgatagcgc cagaactcgc
     2641 ccagctcacc tttgagcggc ttccctgact ggcgaggatc ggcagcaact gcaatgcgta
     2701 aactcataaa atccacaatc cgtcgtgcgt tctgtttgtc catcttgcgt aacgatttga
     2761 gcgcccgatc ggaatagtta atcgtccaga ccaagtgact tcctcacgtc ttcagacgaa
     2821 tgcactgctt catcaccacg gcgaacgcgt gcagcagttt ctgctgaaag ataataatcc
     2881 tccaggtctt ccagatgcgc cagtattgct tcccgcgcat aaaacgtctt tgttctgccg
     2941 gtttgcttcg ccagcgagtc cagacgggac tcaatctcat cagataaccg gatagcaagc
     3001 atataaacct cccgaacacc atttgctata catgtatatc aacagtacca caatcaacct
     3061 aaaagaacaa aaaataatct tgttagcccc tcttgataat ccgtaacgcg gtcaatatat
     3121 ctagcttgag ggctttcctc ttaactattg aaaggataac gattatgggt cttaagccgg
     3181 gtccgaaacg aattgccgat tcaacagggg aacctgataa acgccagcgt gacaataaaa
     3241 aaacgccggg aaatactgac aaattgaaac ccagcaaatc ttcaaaaaaa taagtcagta
     3301 gttaaaagcc ctccggggct tttttgtatc tgccgttcac gctgattaaa cataacaccc
     3361 gttgcccgca ccgccccgga cgggttcgcc gcgaacgggg tggcagtcag gtaaaaccgg
     3421 caacaaacca cccgataaac cgccccgttc gcgacagata aattttacct ggctgcatag
     3481 ccatgcagcc aggtgaatga catccggttt cagcgtaaat gcccgatgag aaccctgttt
     3541 tcctgcattt cagcccagcg acggaccgtc atagcttcgc tgccgggaac gcgcttccgt
     3601 cagttccttt tcgtgctgcc gttgctcgtg cgcccgttct gcggccacgg ccatctcatg
     3661 acgctgcact accggctcaa acgctgcagt tgcccgctca agcgttccac ctgctgactc
     3721 aagcgcgtca cgcgctcgct cagcgccgcg ttctcccgtt gcataagccc tgacattttc
     3781 cgccattccc cgaaggcgct ctcccactcg tccagccttt tcgagtagtc ctgctgtagc
     3841 tgctctaatg cgctcagcaa ctgtttttcc agctcggtca tcggcactta ctccttcacc
     3901 agtctcagcg cgttctgctt cgaccgcccg ttcccgaccg tccagctctg cgtcctgtct
     3961 acggccgtcc ccttcggcag caccagaaaa cgtccgttgc tgtcttccag gtacgtcacg
     4021 ccccaggtct tcgcgttcag cttcgacagc gctgcgttct gccggtcgat ttcggccaga
     4081 tttgacgcta tcaagctccc ctgataccac aacaccccgc tcaggctggc gaacagcagt
     4141 cctgacacca tcgtgatggt cagccatgtc cgcccgacca tacgcatcac gctcaggcgg
     4201 ttcgactgca tggccgcgct cataccctgc tcgtgggcgc ggatggcggc gctgattctc
     4261 ttcccgcttt cgctcagttc cgctctgaca gacttctcgt gctcgctgaa tgcggctttc
     4321 agcatttcgc cggtactctg ctgctgcgct ttcgatttct gctctaagtc cttcgccagc
     4381 gttaaaagac tgttcataga tggctccctt aagtcggatg tttcgccctc cgtccgggtc
     4441 ggcaatgctg atgctgctct ttgtggtgcg cacgacctca aaacctgcgc tttccagcgc
     4501 ctcagtgacg tcctgacgcg ttttaagctc ccctgacgag gcgagggcca gtaagccccg
     4561 cgtaatagcc tctgcggctt cctgcttcgt tttgggtaac gaagatggcg tcaccagcgc
     4621 gcgccggttc tccggtgcgt tcgggtcgtg cagccccagt cgcccgttca ccacggtctg
     4681 ccacgcatcg atgcgcggac ggtctgcccg gtcgtagtac ggctggagac gcctgccggt
     4741 cagcagttcc gtgttcggta ccagaaaatt cagttccagc cgccccttgt cccggtgttc
     4801 aacccacagc acgctgtact ggtctttatc gagtccgggc atcagaaccc gctcgaagct
     4861 cgccatcagc ttttcacgct ggccgggcgg taaatcctgt tcagcaaaag acaggacgcc
     4921 ggaggtgtac tttttggcgt agggcgaggc gtcgataagc tcccggacct cctccggctt
     4981 tccctgcagg acgatggccc cgtcgcgctg gcggtctttc cccagcagat aatccaccgg
     5041 cccggcaccg ccgcctcgcc ctcgcggatg aaacttaacg atcatcgtct gccccctttt
     5101 ccagcacggc atgccgcagc cgctcgagtt cggcatcaat ggccatcagc gcggccacca
     5161 cctgaacccg gtcagcgcct gaccactggc cgccgttaat tttccgggcg atctggttga
     5221 ggttgttccc catcccggca agctggcgca gcaaaaccgg atcgatggac ggcagccgca
     5281 acgaccgcgc cgggcgcgta tcgagacagg tttcccgcat ccacaccgcc agttgccttc
     5341 cattgcagcg ctccagcagc tgctggtact ccccatcggt cacccggata cctaaaaaac
     5401 gggtacgctt aaccgccatc ctctcctccg atatccgcaa aatgtgaccg ggctgaacgc
     5461 tcaagctctg ccatctgctc ccggcggtaa tcttcctcct gtgcctgacg catcagattt
     5521 gccatcactg ccggatgcca ggatcttttt tttacgacga gcggcttctt cttcggcttc
     5581 tgcattatcc ctcctt
//
