LOCUS       NZ_CP095238            10060 bp    DNA     circular CON 05-JUN-2024
DEFINITION  Klebsiella pneumoniae strain 21072329 plasmid p21072329_4, complete
            sequence.
ACCESSION   NZ_CP095238
VERSION     NZ_CP095238.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27363253
            Assembly: GCF_022982715.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   He,Z.
  TITLE     Comparative Genomic Analyses of Klebsiella pneumoniae in Anhui,
            China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   He,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2022) School of life science, University of
            Science and Technology of China, 1129 Huizhou Ave, Hefei, Anhui
            23005, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.4.9b; SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022982715.1-RS_2024_06_05
            Annotation Date                   :: 06/05/2024 03:13:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,790
            CDSs (total)                      :: 5,667
            Genes (coding)                    :: 5,520
            CDSs (with protein)               :: 5,520
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 147
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 0 of 147
            Pseudo Genes (frameshifted)       :: 47 of 147
            Pseudo Genes (incomplete)         :: 102 of 147
            Pseudo Genes (internal stop)      :: 17 of 147
            Pseudo Genes (multiple problems)  :: 18 of 147
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095238.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="21072329"
                     /isolation_source="sputum"
                     /host="Homo"
                     /db_xref="taxon:573"
                     /plasmid="p21072329_4"
                     /geo_loc_name="China: Anhui"
                     /lat_lon="31.85777 N 117.291449 E"
                     /collection_date="2021-07"
                     /collected_by="Zhien He"
     gene            join(8617..10060,1..521)
                     /locus_tag="MU289_RS28745"
                     /old_locus_tag="MU289_28745"
     CDS             join(8617..10060,1..521)
                     /locus_tag="MU289_RS28745"
                     /old_locus_tag="MU289_28745"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            521..1252
                     /gene="mobC"
                     /locus_tag="MU289_RS28750"
                     /old_locus_tag="MU289_28750"
     CDS             521..1252
                     /gene="mobC"
                     /locus_tag="MU289_RS28750"
                     /old_locus_tag="MU289_28750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            1259..1789
                     /locus_tag="MU289_RS28755"
                     /old_locus_tag="MU289_28755"
     CDS             1259..1789
                     /locus_tag="MU289_RS28755"
                     /old_locus_tag="MU289_28755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1816..1995)
                     /locus_tag="MU289_RS28760"
                     /old_locus_tag="MU289_28760"
     CDS             complement(1816..1995)
                     /locus_tag="MU289_RS28760"
                     /old_locus_tag="MU289_28760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2021..2449)
                     /locus_tag="MU289_RS28765"
                     /old_locus_tag="MU289_28765"
     CDS             complement(2021..2449)
                     /locus_tag="MU289_RS28765"
                     /old_locus_tag="MU289_28765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(2613..2927)
                     /locus_tag="MU289_RS28770"
                     /old_locus_tag="MU289_28770"
     CDS             complement(2613..2927)
                     /locus_tag="MU289_RS28770"
                     /old_locus_tag="MU289_28770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2915..3193)
                     /locus_tag="MU289_RS28775"
                     /old_locus_tag="MU289_28775"
     CDS             complement(2915..3193)
                     /locus_tag="MU289_RS28775"
                     /old_locus_tag="MU289_28775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(3368..3733)
                     /locus_tag="MU289_RS28780"
                     /old_locus_tag="MU289_28780"
     CDS             complement(3368..3733)
                     /locus_tag="MU289_RS28780"
                     /old_locus_tag="MU289_28780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(3730..4101)
                     /gene="tolA"
                     /locus_tag="MU289_RS28785"
                     /old_locus_tag="MU289_28785"
     CDS             complement(3730..4101)
                     /gene="tolA"
                     /locus_tag="MU289_RS28785"
                     /old_locus_tag="MU289_28785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(4375..4620)
                     /locus_tag="MU289_RS28790"
                     /old_locus_tag="MU289_28790"
     CDS             complement(4375..4620)
                     /locus_tag="MU289_RS28790"
                     /old_locus_tag="MU289_28790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            4947..6632
                     /locus_tag="MU289_RS28795"
                     /old_locus_tag="MU289_28795"
     CDS             4947..6632
                     /locus_tag="MU289_RS28795"
                     /old_locus_tag="MU289_28795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            6642..6899
                     /locus_tag="MU289_RS28800"
                     /old_locus_tag="MU289_28800"
     CDS             6642..6899
                     /locus_tag="MU289_RS28800"
                     /old_locus_tag="MU289_28800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            6984..7133
                     /locus_tag="MU289_RS28805"
                     /old_locus_tag="MU289_28805"
     CDS             6984..7133
                     /locus_tag="MU289_RS28805"
                     /old_locus_tag="MU289_28805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(7237..7815)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          522..1249
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 tgaacgcgct ggggacgctg cgcagccggg actgcaacct gctgctcgcg caccagtcgc
       61 tgggcgactt cggtcagtgc gggcaggatc tgcccgctga ttttgtgaaa accacggtac
      121 tggataacac gccgatccgc tggttctatc gcgcggccag ccaggagtcc gcgcagtggg
      181 cggcgggcca gaccggggag atccgggtgg acgtggagcg ccgccgggcc agccgggagg
      241 cggggaacgt ggagcacatc agcggcgaca gcttcatcca gaaggaggcg cggccgctgt
      301 ttgacgtgaa caccctgcaa cacctgcctg acgggttcgc ggtgatgacc gggctgggcg
      361 tggcgcggct ggcgttcagc agcccgctgc gggttgagcg ccgggaaatt tcgctgaaaa
      421 ccttcccggt gctggcgaaa acggacccgc tggcggagta cctgcctccg gccgacggcg
      481 gcggaccaaa agcggatgat gattttgcgg ggctttactg atgacactgg agcagtacaa
      541 cgtcagccac gcgaagcggc aggcgcggaa cgcggagaaa acacggctga ccctgcgctg
      601 gctgcgggaa gagctgtgtt ccacggcgga gctggttgcg cgacggctgg ggattgcggc
      661 ggtacagccg gtttaccgct tcctggacag tctggtggcg aaagggctgc tggtccgggc
      721 gaaatacccg gttgacggcc gtcaggtcag cgtgtggggg ctgacgccgc acggggtggc
      781 gttctcgttc gacgaggacg agccgctgac ggacgttatt ccgtttcagc cgtcaagggt
      841 gtcggcggcg cagctgccgc accggctggc cgtgcagtcc ctgcgcctct ccatggaggg
      901 ccggggggcc accggctggc ggtacctgca ccgtatggcc ctgaaaggga tgaaagtgcc
      961 ggacgcgctg gcggagctgg acggcagaac ggtggcattt gaggtagagc gcaccgtcaa
     1021 gagccgcagg cggtatcagg aagtggtgtc cgggtatctg ttcaaccgcc gggcgaacgg
     1081 catcgatgaa atctggtaca tctgccctga ccggccgacg cttgtccggg tacagcgggc
     1141 gattctggcg gtggatgaaa tcgtgaatcc acagaccggc gaggcccgca aaacggtgga
     1201 gctggaccgg gagaggctgt tcgcgtgctt taaatttatg acaacggagt gagggattat
     1261 ggcgctgtac atggtattta ttgaggacgt ggaagacaaa gacggcgagc gctgcattat
     1321 gccaaccagc gagcatctgg aagacatggt ggtgctcgcc tggcagaccg gggcgtatgt
     1381 tttctgtttt gtcgaccggg cccagcatct ggcgatgatg aaatccatcg ggatgtatga
     1441 cgagcggctc aacagcgacg aaaaagccac gtactttttc accggtgaaa atccgcaatg
     1501 ggaacgggac aaacccacag gaatgaagct gtgtgattac ctctatcgaa aggtggagcg
     1561 gttcgccatc gagaacggtg tgcaggtggt tgatgccggg aagtgctcac cggagcagat
     1621 taacgcgatg attgaggtgg tcagcgagcg gcacggggaa agcgcggtga acgagaagct
     1681 gaaagcggag ctgatgcggg aatataacag ccgctatttt aacgtgccgg tgcctgaact
     1741 gccggaggac aaacccgccc gccggtggtg gccgttcggc aaaaaataac gccagcaccg
     1801 gcatggccgg tgctgttatt cttccccgag cttatcccgc atcacctgcc agagtgtttc
     1861 cgcatcctcg tgaagccgtt cgcacatgtc ggccggggta tccatgtcca gctcgttcag
     1921 cttttccagc aacagcagcg actgatcctt aatgaattta gccatattga gggcagtttt
     1981 ttcctgcttc gtcatgttct ccattccctt cggtaagttc tcagctaaac cagccggacc
     2041 agaagcgttt tttcttctgt gtcagttctg ttgtcaatct gtccacttcg gcctggagtt
     2101 tccggcgctc atcatcgtgc tggcggcgtt cttcttcccg ggatgtttta tcttccagca
     2161 tcagggtgac agcctgccgg agatcggcca gttccttttg cattgccaga agctgttcct
     2221 gtgggaaatt tttttgtgta ttttcacggt gtgaaatgcc gggaatttgt ttctcaggga
     2281 tattcacatt tccgtataca cggatcagtt ctgacacatc cacgacaggg ttgtttttcc
     2341 cgtcacgtga cacggtaacc tttccctgtt ttacatgatt gtatagcgtt cttctggtaa
     2401 tgcccgcggc ttgtgctgcc tgtgaaaggt tgagcaatgt tttcgccact gtatacccct
     2461 gattttatcg ggtgtgaaag gtgtgtaatt tctcacggta aatgtatcgc tgaattttac
     2521 ccgtttttcc gttcaggatg tggttttctg acgcgctgcg cttgtcaaac cggcgacggc
     2581 cacgcaatga attgcgcccc cgtcatcggt tattagaggc tgtctgcccc cggatacccg
     2641 gattctttct gatgcctgac ggtgacgatc acaatctggt ccatttcccg gtcgtggcgg
     2701 tagagcatca catagccact gtcgccaaac ccgatcacca gttccttata ttccagcggc
     2761 agaaacggca ccgggcggcc aatgtctggc aatgttttca gttgctggat agcccggacg
     2821 atcacctcac ctccttttct ggctgccagc cgatttttgg ttttgagaaa gtcctgaagg
     2881 cgttgtaaat cgcgctgtgc cagcgcggaa atcgttacct gtggcatggc ggagcatcct
     2941 gctcgttctc agttccccag gtacttatcc aggcttccgc ttcttccgca gtaaggtgca
     3001 gacccgtttc ctgatagtgc tgccatgcgg cctgtccgtc ccgcaggtac tggctgcgct
     3061 tctcttcccg gtcgatgtat tcggtgattg cctccagcat tagcgcgtgc gctgagcggt
     3121 gccggtcatc ggccagcgtt ttcagccggt ccttaagttc ttcatcgagc ctgatggatg
     3181 ttgctgatga cataaaagcc cctttgtagt catatgtatt acacatgact actttaatcc
     3241 gtggcggcgc tatttgccag aaatacggct tttgctgccc atttttcagc ttcgcttcca
     3301 ctaatcaggg ctgctacagg ctcactcatc atccaggact attgcagcct gcgggttaaa
     3361 ggacctgtta ctggcggcaa ccatcatcat tgagcttttg ggtggtgact ggtcgcggat
     3421 cctgtaagcc gtcatttttc agactgaatt caaaatttat aacctgtcgc attgccgggt
     3481 gccctgcggg atatagccag tgcctcattg ccgtcagcgt ttcccgaaaa aaaaggtgac
     3541 agccggtaag cgacacgttg tccactcgtc catcgtccag aatatcaaac gtggcggtca
     3601 cctgaccttc gatgcgtttt gctgcggcct gtaccgggta ggttggctgc agtgtggtta
     3661 cgggttttag tggtatcgca tgtaccggag tgaggaacat ggccgccagc actgtcagag
     3721 taaaagcttt catggtctga aatccagagg ggcgtttttg aatatttgcc aggtattttc
     3781 atccggtgcc ggcggaattt tggcgtgggt aacggctgat attgctgctt tacagagttt
     3841 cggatctcct ccttcagcaa tggcgctgtt cagagtgcca tcacgttgta tggatatccg
     3901 cacggtgcac atttttcccg cataggcctc tggttcaaac atctgcattt caacagcggt
     3961 cttgatggcc gcagcgtagc tactgatttt agatgactta tccagagtat aaattttcgg
     4021 cggctgggtg gtggaatgct tacaggccgt caggagtaag gggctgcaca atgccagcag
     4081 gataaagggt attaaacgca tcgggttgat gacctgtcag taaaaaatgt tacatgtata
     4141 tttgtcactc tttacggcca cgttcattta cataaacacg gcttatcgta tcgtttttac
     4201 accaaacggc ccgccatgca gttgcgtgca taggcatgca gcgggttaca atttaccggg
     4261 cgcgtttccg gcggttttcc gggtggtttg ttgcctgttt tatcccgtag ccgccggaaa
     4321 cgccctcaga ccgtctgagc ggtgcgggca atgcgacgtt atggtaaata ccccttaccg
     4381 gcggcgctgt ggctgccagg tgaaggtggt ttccgtagcc tcgtatttct cccgacgcat
     4441 ttcctgcgcc cgcctggctt cacgttcgtc ctgaatggca cgaagcaccc tttcccgcac
     4501 ctgttgtttt accgcctttg gcagcgtttt gttccctttc aggcccatgg ccagtgcctg
     4561 agcgacaaag gcgtccgcca ggcgcttttc ttcagcggga gtcagtttca ggtgcgtcat
     4621 agtggcctcc gggcgtctca ggtttcccga acagtttaac cgcaggtcct ctcctcatgc
     4681 cagttagctt tcagatgtgt ctataccatc tgaaatgtaa gtgtgatgaa tgtcttaaat
     4741 aataaaattt gttgccatga gaaattttcg ggaaaagacg cgatctgctt acaggtggct
     4801 ccgggcgctt ttttgtgcct gaagatgaga actaatctca tatattgaca cctgaaaacg
     4861 ggaggagtaa ggtaataatt atactgtgta tatgtacagt atgttggctt taatttattg
     4921 ttttaaatgt caaagaggaa aacggtatga gtggtggaga cggtcgaggt ccgggtaatt
     4981 caggtctggg acataatggt ggtcaggcca gtgggaatgt gaacggtacg tctggtaaag
     5041 gtggcccttc atcaggtggt ggtacggatc caaacagcgg gccgggctgg ggtacgacgc
     5101 atacgccgaa tggagatatt cataactata atccggggga gtttggtaac ggcgggagta
     5161 aacccggtgg taatggtggt aacagcggca atcatagcgg tagctctggt ggcggacagt
     5221 cttcggccac tgcgatggcc ttcggtctgc ctgccctggc tactccgggg gctgaaggac
     5281 tggctttatc agtttccggc gatgcgttgt cggccgccgt tgctgatgtg ctggctgccc
     5341 tgaaggggcc gtttaagttt ggtctgtggg ggattgcgat ctacggtgtg ctgccttctg
     5401 agattgcaaa agatgatccg aaaatgatgt caaaaattat gacgtcatta ccggccgatg
     5461 cggtgacgga gactccggca agtactttac cactggacca ggcgacggtt cgtgtcagac
     5521 aacgggttgt ggatgtggtg aaggatgagc ggcagcatat tgcggttgtc gcaggtcggc
     5581 caatgagtgt ccctgtggtg gatgcgaaac cgacaaaacg tccgggggta ttcagtgtgt
     5641 cgattccggg tctcccgtct ctgcaggtga gcgtaccgaa aggtgttccg acagcgaaag
     5701 ccccgccaaa aggcattgtt gctgaaaaag gtgattcacg tccggctggt tttacagccg
     5761 gtggtaactc ccgtgaggcc gttattcgtt tcccgaaaga gaccggacag aagccggttt
     5821 atgtgtcggt gacagatgtt cttaccccgg cacaggtaaa acagcgtcag gaggaagaaa
     5881 agcgtcgcca gcaggcatgg gacgccgctc atccggaaga ggggctgaaa agagactatg
     5941 ataaagcgaa agctgagctg gatgccgaag ataaaaatat tgcgacctta aacagccgca
     6001 ttgcatcgac agagaaggcg ctccccggtg caagggctgc tgttcaggaa gccgataaaa
     6061 aggtaaaaga ggcagaggca aataaagatg attttgtgac ttataacccg cctcatgaat
     6121 atggctccgg gtggcaggat caggttcgct atcttgataa ggatattcag aatcagaatg
     6181 agaaattaaa agcggctcag gcatctttaa acgcaatgaa tgaatcctta tccagagata
     6241 aggctgcact taccggggcg atggagagcc ggaaacaaaa ggagaaaaaa gcgaaggatg
     6301 cagaaaataa gttaaatgag gaaaagaaca agcctcgcaa ggggactaaa gactacggcc
     6361 atgattatca tccagtacct aaaacagaag acataaaggg acttggagag ttaaagcgag
     6421 gtgatcctaa aacacctaaa caaggtggtg gtggtaagcg ggcccgatgg tatggcgata
     6481 aaaaacgtaa aatttatgaa tgggattccc agcacggtga gcttgaaggg tatcgtgcca
     6541 gtgatggcga acacctaggc gcatttgatc ccaaaacggg taagcaggtt aaagggccag
     6601 atccaaaacg gaacattaaa aaatatcttt gagaggtaaa tatggggctt aaattacgtt
     6661 tgacatggtt tgataagaaa actgaagagt ttaaagggga agagtattct aaagactttg
     6721 gtgacgatgg ttctgtcatt gaaagcttgg gaatgccttt aaaggataat attaataatg
     6781 gtgagtttga tttgaagaaa gaatgggttc ctttactaca gccatttttt aaaaatcaaa
     6841 taaaacctga tgtggataat tgttatatag cttttgatta tcgggacggt aactggtaac
     6901 aaaaaattat caaggatgat ttgaaatact gacttccggt gttcagggat gaatgccgga
     6961 gtctttaaat acagggaaag tatatgaaaa aggtaaagac tatttttctt tttatattga
     7021 ttgttgctgg ctttcttctg gccgcatgtc aggcaaatta tatccgggat gttcagggcg
     7081 gaacggtggc accatcgtcc tcctctgaac tgacggggat cgcggttcag tagaaaagat
     7141 caaaggatct tcttgagatc ctttttttct gcgcgtaatc ttttgccctg taaacgaaaa
     7201 aaccacctgg ggaggtggtt tgatcgaagg ttaagtcagt tggggaactg cttaaccgtg
     7261 gtaactggct ttcgcagagc acagcaacca aatctgtcct tccagtgtag ccggactttg
     7321 gcgcacactt caagagcaac cgcgtgttta gctaaacaaa tcctctgcga actcccagtt
     7381 accaatggct gctgccagtg gcgttttgcg tgctttcccg ggttggactc aagtgaacag
     7441 ttaccggata aggcgcagca gtcgggctga acggggagtt cttgcttaca gcccagcttg
     7501 gagcgaacga cctacaccga gccgagatac cagtgtgtga gctatgagaa agcgccacac
     7561 ttcccgcaag ggagaacggc ggacaggtat ccggtaaacg gcagggtcgg aacaggagag
     7621 cgcaagaggg agcgacctac cggaaacggt ggggatcttt aagtcctgtc gggtttcgcc
     7681 cgtactgtca gattcatggt tgagcctcac ggctcccaca gatgcaccga aaaagcgtct
     7741 gtttatgtga actctggcag gagggcggag cctatggaaa aacgccaccg gcgcggccct
     7801 gccgttttgc ctcacatgtt agtcccctgc ttatccacgg aatctgtggg taactttgta
     7861 tgtgtccgca gcgcccgccg cagtctcacg cccggagcgt agcgaccgag tgagcgagga
     7921 ggcgcaattt ccccggcacc tgcaagacgg tcaccggacg cgctgatgtg ggatattgtc
     7981 ccctgattaa gtctttctat tccctgacga tcaattatca ggataggtga accatgtcgg
     8041 tttttcgctg aacattttac gttaacctat ctctgttgct tttttctcct tgcggagaaa
     8101 tgcaagtcat ccccgctggg gcgtggctga cttcagggga aaagaccttg cttacgctgg
     8161 ctggacgcca ccgacgcacg gtcttttccc ctctttctgg ctctgtccgg ctggacgccg
     8221 gccacgccag aaattcaccc ggcccccgtc aggctcacgc ctgaccaccc acaaaaatcc
     8281 acggtgctgg acgcacctga cgaagcagca aagctgctcc tgattttgcg ggtggtcagc
     8341 cgctcgctat ggcgaactct gacgggggcc ggaacaccgg aaaagaccgc actggggtgc
     8401 cgccttttcc tctcacacgc tggggcgtgc tcgttgttcc tccggggctg cgccttttcc
     8461 gcatggggcg gaaagaggct ggcctccgcg gtgaaagctg aacgcaatca ccacggacgt
     8521 gaccgccgcc gccatgcctt ggggcatgtc cgcggcttgt gcagcggggg ctgcgcatca
     8581 cgccggtaaa cccgtcgtct gaaggagaga gattgcatgt ttaatacgga ttcgcttgcc
     8641 tggcagtggc atatcccgga gctggcccgg cagatttacc cgtgggtcag ctggctgtac
     8701 cgggagtatt acagtctggc cggttttttt ctggttatcg gcctgttttt cggtctggct
     8761 gaactctggg ccggccgaat gcgttttgtg gggctgagcc ataaggcgcg gcgggagtat
     8821 gccgatcgct ataagcgcga taataaccgg actgtgccat tttcggtgct ggcctcgctg
     8881 gattcacccg ggcggatggt cagggcacgg cgacatgtct ggaactacgc gtactggctg
     8941 accggcgctg ccggtctggc ccttctgggg gggctggttg tcgccgtgtt tatcgagagc
     9001 tggggagaaa ccgtcctgcg cccggacctc tatgcgaacg cagattatta cctgctgcag
     9061 ccggctaacc tgttcgggat agcctggtgt gccggagggc ttgtcgccgg cgtccttctg
     9121 gcgcggctgc tggcgttcct gctgcttgat ggtcattttg cggccgctga cgaggcggtg
     9181 aacgcgaaac tgaatcagga gagccggcgc agcagccagc ggaccggaga aatgacagac
     9241 gtccgccacc tgcatttcgg ggagccggta ccggttaacg cgctggccga tttttccacg
     9301 gagcaggccc ggaaacagca ggcggtgttt ctgggtaagg atgagcaggg ccagccggtg
     9361 ctggtccctc gtgatacctg gcgcaagacc aatattcaga tcctcggcct gcccgggagc
     9421 ggcaagagcg cgatggccac caacgcgctc attcgctgcg tacgcgattt cggggacgct
     9481 gtggtgtact tcgaccccaa aggggacgcg tgggcgccgc acgtcttccg ggcgcactgc
     9541 ccggacttca ccctgctgga tctgcggccg gggaaaccgg cgcagctgaa tctgttccgc
     9601 gatctcgacc agtacgccct gaagaatctg ctggttgccg ggtttaacct gagtgagacc
     9661 ggcgacgtgg cggaccacta ccgcatcagc gagcagaaag cggcgaagct gattgcggag
     9721 cagttcccgc agggggcaaa cattcagcag gtgctggcgg cggcgtacgc gttgccggag
     9781 gccctgaaaa aggacgtgaa ggggctgatc accaagctgg agaacgtggc cgacctgagc
     9841 gtcctgcaga cggacagcgg tatcgatgtg gcggggataa tcaacggcgg cggctgcctg
     9901 tacgtcatcg gctcgatgga cgatgaggcg gtgatccggg tgcagaagat gctgtttgcc
     9961 cgctgcgcgc aaatcatcat tgcgcgggat gagttccgga ggtggccgca cgcgagcatc
    10021 atgctcgatg agattaaata tctgctgtcg aagtacgtgc
//
