LOCUS       NZ_CP095179             2493 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain K528 plasmid pK528_2k, complete
            sequence.
ACCESSION   NZ_CP095179
VERSION     NZ_CP095179.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27363748
            Assembly: GCF_023375605.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Yin,Y.
  TITLE     Potential Transmission of mcr-10-Harboring Enterobacter spp. among
            Companion Animals and Humans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Yin,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2022) Baisc Veterinary, Yangzhou University, No.
            48, wenhui east road, hanjiang district, Yangzhou, Jiangsu 225000,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023375605.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:43:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,682
            CDSs (total)                      :: 4,568
            Genes (coding)                    :: 4,468
            CDSs (with protein)               :: 4,468
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 34 of 100
            Pseudo Genes (incomplete)         :: 63 of 100
            Pseudo Genes (internal stop)      :: 17 of 100
            Pseudo Genes (multiple problems)  :: 13 of 100
            Pseudo Genes (short protein)      :: 1 of 100
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095179.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="K528"
                     /host="healthy dog"
                     /db_xref="taxon:158836"
                     /plasmid="pK528_2k"
                     /geo_loc_name="China: Jilin"
                     /collection_date="2019"
     gene            complement(47..274)
                     /locus_tag="MUY31_RS23385"
                     /old_locus_tag="MUY31_23385"
     CDS             complement(47..274)
                     /locus_tag="MUY31_RS23385"
                     /old_locus_tag="MUY31_23385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1480..1698
                     /locus_tag="MUY31_RS23390"
                     /old_locus_tag="MUY31_23390"
     CDS             1480..1698
                     /locus_tag="MUY31_RS23390"
                     /old_locus_tag="MUY31_23390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(1989..2174)
                     /locus_tag="MUY31_RS23395"
                     /old_locus_tag="MUY31_23395"
     CDS             complement(1989..2174)
                     /locus_tag="MUY31_RS23395"
                     /old_locus_tag="MUY31_23395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          1855..1942
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(690..1218)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 aggtaaagaa aaaggccaca ttagcggcct ttttcggaga agatgctcag tgcggaattt
       61 cggagacttc tttaacgtcg gttttattga tctgctgttt gacaccattc gcgtctgtat
      121 agccaagcag gcctgagtct gattctttag gtttgccgtc gctgacaata gtgcgaccgt
      181 cattggtgtg tatggcatag cttgtgcgcg tgcagccggt gagggctgaa agcgcaactg
      241 ccgctgcgaa cactgaaaca aaaatctttt tcaaagccag ctccttttat ccatatcagt
      301 tttggtcact aaccatctaa ctataggtca ttttttatgt gaaaagaggc ttgatggtgg
      361 gctgtatatc gagttaggcg cgaggtgcta tggtcaaagt gtggtgtcag gcggggagga
      421 ggcttatatg agatgtaatc cggccgatac atataccaag atgatttaag tattcctctt
      481 tgtgaggttg gatgtctaat ttataaagga tcttcttgag atcctttttt tccgcgcgta
      541 atctcttgcc ctgtaaacga aaaaaccacc ctggcaggtg gtttttcgaa ggttaggtaa
      601 tcctggcaga tcccctaacc gtggtaacag tcttgtgcga gacatgtcac caaatttgtc
      661 ctttcagtgt agcctcacta aggccgccac ttcaagaact cttgagacat ctctcgcaca
      721 tcctgtttgc caatggccgt tgccaatggc gattagtcgt gtctttccgg gttggactca
      781 agttgatagt taccggataa ggcgcagcgg tcggactgaa cggggggttc gtgcatacag
      841 tccatcctgg agcgaactgc cttcccggaa ctgagtgtca ggcgtggaat gagaaaccgc
      901 ggccataaca gcggagtgac accggtaaac cgaaaggcag gaatgcgggg gagcacgagg
      961 gagccaccag ggggaaacgc ctggtatctt taagccgcat cgggtttcgc caccactgat
     1021 ttgagcgtca gattctgtga tgcttgtcag gggggcggag cctatggaaa aacggctttg
     1081 gcgcggcctt atgctttctt cgttaagtat cttcctggca tcccccagga aatttctgat
     1141 ccatccgtaa gcccgtcccg ctcgccgcag ccgaacgacc gagcggagcg agtcagtgag
     1201 cgaggaagcg gaatatattc tgtatcacat tttctcctga cgcgttttct ttcactttct
     1261 gcgcctgtct tatgtggcat taatgctatg tgttactgcc atgctacatc ttaagccagt
     1321 atacactccg ctagtgctcc gtgactggtc cggcgctgcg cccggaaccc gcctgtaccg
     1381 gttcagcagc cgttccggcc tgactgcaat tttttttttc atccctgccc gctaccctgt
     1441 aaacctttct tctgcgttgc cgttaacagt aggtaagcca tgtttggttt ttcttccgga
     1501 gccctgatga ctcttagtga tgatgagcga cacctgcttg tgtccgttgt ttcggtctgg
     1561 ctacggcgtg ccggagggga tgccggagcc atgatgctgg atgcataccg gcagattttg
     1621 tctgaaaccg aaccggccgt aaggactgtc atgctcgaat ttcttgaatc cgttcgcatt
     1681 cattacattt cgtcctgagc attttctgta tttctgtcca tacttaaacg aggcggccgt
     1741 cacggacggt ggtcgtcgtt ccatgatgct gttatggacg agtgagagta atctaatcta
     1801 acttgattga gttccgggcg tgccgcccgg ttttttttgc tgtttcccgg tgtccgttcc
     1861 gggacaggat gtgcatttgt aataccgcgc acgcgccggc atcacaaata ccctggtcag
     1921 ggctgcgccc cgacaccccg ccatcgcctc acggccgcgc actgaattgc gcacccgttc
     1981 accgattttc acgcattttc ctcgcctatc tgtgcgttca gcatggcgta aagcgcttcg
     2041 gcctgatcgt gcaggtgctc acagcagccg gcagcctcat ccagatccat ctgctcgagc
     2101 cgctcaagga ggagaagtgt ctgtgcgcgg ataaatttgg ccatattgag ggcggcggat
     2161 tcctgtctgg tcactggtga ttttccccgt ctggtgatga tttttgcgtg aaaagagcat
     2221 ggcgtcattc tggtgcatag tcatgcatgc cgttaaaatt taacaggcgc gtttctgacg
     2281 ggttccgggg tggtttgttg tggtttttgt catggtttcg tcaggaacgc gctgaacgcg
     2341 tctcaggcgg tgcgggcaac ggatgttatg gtaaattctt gggggcttga ttataaaata
     2401 ccgcacttcc tcttatgtct tgtgcttttt agacaaccct gaaagctttt agctttggtg
     2461 atgcaatttg ctgggatttt ggcggagaac cac
//
