LOCUS       NZ_CP095176             2495 bp    DNA     circular CON 21-MAY-2024
DEFINITION  Enterobacter roggenkampii strain K666 plasmid pK666_2k, complete
            sequence.
ACCESSION   NZ_CP095176
VERSION     NZ_CP095176.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27363726
            Assembly: GCF_023375585.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Yin,Y.
  TITLE     Potential Transmission of mcr-10-Harboring Enterobacter spp. among
            Companion Animals and Humans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Yin,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2022) Baisc Veterinary, Yangzhou University, No.
            48, wenhui east road, hanjiang district, Yangzhou, Jiangsu 225000,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023375585.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 04:40:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,080
            CDSs (total)                      :: 4,962
            Genes (coding)                    :: 4,851
            CDSs (with protein)               :: 4,851
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 52 of 111
            Pseudo Genes (incomplete)         :: 48 of 111
            Pseudo Genes (internal stop)      :: 27 of 111
            Pseudo Genes (multiple problems)  :: 13 of 111
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095176.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="K666"
                     /host="healthy cat"
                     /db_xref="taxon:1812935"
                     /plasmid="pK666_2k"
                     /geo_loc_name="China: Jilin"
                     /collection_date="2019"
     gene            43..234
                     /locus_tag="MUY33_RS25355"
                     /old_locus_tag="MUY33_25355"
     CDS             43..234
                     /locus_tag="MUY33_RS25355"
                     /old_locus_tag="MUY33_25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021243041.1"
                     /translation="MTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQILSETEPAV
                     RTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(525..710)
                     /locus_tag="MUY33_RS25360"
                     /old_locus_tag="MUY33_25360"
     CDS             complement(525..710)
                     /locus_tag="MUY33_RS25360"
                     /old_locus_tag="MUY33_25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1076..1303)
                     /locus_tag="MUY33_RS25365"
                     /old_locus_tag="MUY33_25365"
     CDS             complement(1076..1303)
                     /locus_tag="MUY33_RS25365"
                     /old_locus_tag="MUY33_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          391..478
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(1719..2247)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 aacagtaggt aagccatgtt tggtttttct tccggagccc tgatgactct tagtgatgat
       61 gagcgacacc tgcttgtgtc cgttgtttcg gtctggctac ggcgtgccgg aggggatgcc
      121 ggagccatga tgctggatgc ataccggcag attttgtctg aaaccgaacc ggccgtaagg
      181 actgtcatgc tcgaatttct tgaatccgtt cgcattcatt acatttcgtc ctgagcattt
      241 tctgtatttc tgtccatact taaacgaggc ggccgtcacg gacggtggtc gtcgttccat
      301 gatgctgtta tggacgagtg agagtaatct aatctaactt gattgagttc cgggcgtgcc
      361 gcccggtttt ttttgctgtt tcccggtgtc cgttccggga caggatgtgc atttgtaata
      421 ccgcgcacgc gccggcatca caaataccct ggtcagggct gcgccccgac accccgccat
      481 cgcctcacgg ccgcgcactg aattgcgcac ccgttcaccg attttcacgc attttcctcg
      541 cctatctgtg cgttcagcat ggcgtaaagc gcttcggcct gatcgtgcag gtgctcacag
      601 cagccggcag cctcatccag atccatctgc tcgagccgct caaggaggag aagtgtctgt
      661 gcgcggataa atttggccat attgagggcg gcggattcct gtctggtcac tggtgatttt
      721 ccccgtctgg tgatgatttt tgcgtgaaaa gagcatggcg tcattctggt gcatagtcat
      781 gcatgccgtt aaaatttaac aggcgcgttt ctgacgggtt ccggggtggt ttgttgtggt
      841 ttttgtcatg gtttcgtcag gaacgcgctg aacgcgtctc aggcggtgcg ggcaacggat
      901 gttatggtaa attcttgggg gcttgattat aaaataccgc acttcctctt atgtcttgtg
      961 ctttttagac aaccctgaaa gcttttagct ttggtgatgc aatttgctgg gattttggcg
     1021 gagaaccaca ggtaaagaaa aaggccacat tagcggcctt tttcggagaa gatgctcagt
     1081 gcggaatttc ggagacttct ttaacgtcgg ttttattgat ctgctgtttg acaccattcg
     1141 cgtctgtata gccaagcagg cctgagtctg attctttagg tttgccgtcg ctgacaatag
     1201 tgcgaccgtc attggtgtgt atggcatagc ttgtgcgcgt gcagccggtg agggctgaaa
     1261 gcgcaactgc cgctgcgaac actgaaacaa aaatcttttt caaagccagc tccttttatc
     1321 catatcagtt ttggtcacta accatctaac tataggtcat tttttatgtg aaaagaggct
     1381 tgatggtggg ctgtatatcg agttaggcgc gaggtgctat ggtcaaagtg tggtgtcagg
     1441 cggggaggag gcttatatga gatgtaatcc ggccgataca tataccaaga tgatttaagt
     1501 attcctcttt gtgaggttgg atgtctaatt tataaaggat cttcttgaga tccttttttt
     1561 ccgcgcgtaa tctcttgccc tgtaaacgaa aaaaccaccc tggcaggtgg tttttcgaag
     1621 gttaggtaat cctggcagat cccctaaccg tggtaacagt cttgtgcgag acatgtcacc
     1681 aaatttgtcc tttcagtgta gcctcactaa ggccgccact tcaagaactc ttgagacatc
     1741 tctcgcacat cctgtttgcc aatggccgtt gccaatggcg attagtcgtg tctttccggg
     1801 ttggactcaa gttgatagtt accggataag gcgcagcggt cggactgaac ggggggttcg
     1861 tgcatacagt ccatcctgga gcgaactgcc ttcccggaac tgagtgtcag gcgtggaatg
     1921 agaaaccgcg gccataacag cggagtgaca ccggtaaacc gaaaggcagg aatgcggggg
     1981 agcacgaggg agccaccagg gggaaacgcc tggtatcttt aagccgcatc gggtttcgcc
     2041 accactgatt tgagcgtcag attctgtgat gcttgtcagg ggggcggagc ctatggaaaa
     2101 acggctttgg cgcggcctta tgctttcttc gttaagtatc ttcctggcat cccccaggaa
     2161 atttctgatc catccgtaag cccgtcccgc tcgccgcagc cgaacgaccg agcggagcga
     2221 gtcagtgagc gaggaagcgg aatatattct gtatcacatt ttctcctgac gcgttttctt
     2281 tcactttctg cgcctgtctt atgtggcatt aatgctatgt gttactgcca tgctacatct
     2341 taagccagta tacactccgc tagtgctccg tgactggtcc ggcgctgcgc ccggaacccg
     2401 cctgtaccgg ttcagcagcc gttccggcct gactgcaatt tttttttttt catccctgcc
     2461 cgctaccctg taaacctttc ttctgcgttg ccgtt
//
