LOCUS       NZ_CP095175             3103 bp    DNA     circular CON 21-MAY-2024
DEFINITION  Enterobacter roggenkampii strain K666 plasmid pK666_3k, complete
            sequence.
ACCESSION   NZ_CP095175
VERSION     NZ_CP095175.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27363726
            Assembly: GCF_023375585.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Yin,Y.
  TITLE     Potential Transmission of mcr-10-Harboring Enterobacter spp. among
            Companion Animals and Humans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Yin,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2022) Baisc Veterinary, Yangzhou University, No.
            48, wenhui east road, hanjiang district, Yangzhou, Jiangsu 225000,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023375585.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 04:40:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,080
            CDSs (total)                      :: 4,962
            Genes (coding)                    :: 4,851
            CDSs (with protein)               :: 4,851
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 52 of 111
            Pseudo Genes (incomplete)         :: 48 of 111
            Pseudo Genes (internal stop)      :: 27 of 111
            Pseudo Genes (multiple problems)  :: 13 of 111
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095175.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="K666"
                     /host="healthy cat"
                     /db_xref="taxon:1812935"
                     /plasmid="pK666_3k"
                     /geo_loc_name="China: Jilin"
                     /collection_date="2019"
     gene            387..500
                     /locus_tag="MUY33_RS25335"
                     /old_locus_tag="MUY33_25335"
     CDS             387..500
                     /locus_tag="MUY33_RS25335"
                     /old_locus_tag="MUY33_25335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_094312007.1"
                     /translation="MHEPPVQSDCCVLSGNYRLESNPEKHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(726..1505)
                     /locus_tag="MUY33_RS25340"
                     /old_locus_tag="MUY33_25340"
     CDS             complement(726..1505)
                     /locus_tag="MUY33_RS25340"
                     /old_locus_tag="MUY33_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007367323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3825 domain-containing protein"
                     /protein_id="WP_218828005.1"
                     /translation="MDIELFKFAFFPDFENRIKELAENLADKENWDFSTGGRKNSILKN
                     YIFHTFSKIKEENKIVFSAGNAHACFNTGLVTSNWEEIVAFFEKTKNTKSRQPYFFKGF
                     FKISHRNITDKFNNTPQVADYFKNSGDFIFNPHLEIKADIAHIISDNIDRFPANFKTMP
                     EMQVRTILDGAIKTTVNRLKTNYTIAVPQYYGGSIQLLIPLHLTYSGANPDLALVVNRS
                     EGQQFYTSRTCLTLEMAYNNARLIVKPLSSWLSADKI"
                     /name="DUF3825 domain-containing protein"
     gene            complement(2482..2883)
                     /locus_tag="MUY33_RS25345"
                     /old_locus_tag="MUY33_25345"
     CDS             complement(2482..2883)
                     /locus_tag="MUY33_RS25345"
                     /old_locus_tag="MUY33_25345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_102776604.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKTGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2883..3074)
                     /locus_tag="MUY33_RS25350"
                     /old_locus_tag="MUY33_25350"
     CDS             complement(2883..3074)
                     /locus_tag="MUY33_RS25350"
                     /old_locus_tag="MUY33_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_249489063.1"
                     /translation="MTKQQKTALNMATFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          93..500
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.577"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tagggatgtt ctcttttccg cttcctcgct cactcagtcg ctacgctcct gccgttcggc
       61 tgcggcgagc ggcaggagta tgcgtataaa agctcgagaa aaccgagcga tagcgtgatg
      121 gggagaagga gaggccgcac agcggccgtt tttccatagg ctccgccccc ctgacaagca
      181 tcacagaaac tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatatca
      241 ggcgtttccc cctgatggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg
      301 tgtcattccg ctgttatggc cgcgtttatc tcattccacg cctgacactc agttccgggt
      361 aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gactgctgcg
      421 tcttatccgg taactatcgt cttgagtcca acccggaaaa acatgcaaaa gcgccactgg
      481 cagcagccac tggtaactga tttagaggag ttagtcttga agttatgcgc cagttaaggc
      541 taaactaaac gaacagtttt ggtgactgag ctcctctata ctagccagtt acctcggttc
      601 agaaacttgc cggttgctga accctcgaag aaaccacctt gccgggtggt tttttcgttt
      661 aacgaccaac aaaatacgct gctactgata aatccgtcaa tagcaccata tcatgttgtg
      721 gctttttata tcttatcggc agacaaccaa ctgctaagtg gtttcactat caacctagca
      781 ttgttatatg ccatctctaa tgttaagcat gttctggatg tgtaaaattg ctgcccctct
      841 gaccggttca cgacaagagc caaatcaggg tttgcccccg agtaagtcag atgcaaaggg
      901 ataagtagct gaatgctacc gccatagtac tgcggaacag caatagtata atttgtctta
      961 agcctattca ctgtggtttt tattgcgcca tccaatatag tcctaacctg catttcaggc
     1021 attgttttga aattagcagg aaacctgtct atattatcag atataatgtg agctatatcc
     1081 gcctttatct caagatgagg gttaaatata aaatcacctg agttcttaaa ataatcggca
     1141 acctgtggtg tattattaaa tttatcagtt atatttctgt ggcttatttt gaaaaaacct
     1201 ttaaagaaat aaggctgcct tgactttgta ttttttgtct tctcaaagaa agcgactatc
     1261 tcttcccagt tgcttgtaac aagcccggta ttgaaacacg catgtgcatt cccagcagaa
     1321 aagacgattt tattctcttc ttttattttg ctaaaggtat gaaagatata attcttcaaa
     1381 attgaatttt ttctaccgcc tgttgagaag tcccagtttt ctttatcagc cagattctcg
     1441 gccagttctt ttatcctatt ttcaaagtca ggaaaaaaag caaatttaaa aagctcaata
     1501 tccataattt tctccaaaaa gaaatgccac cagttaactg gtggcattct atatgattaa
     1561 atgtattttt gcttgattgc caagcattct tggatagacc caagcgtctt tataaactga
     1621 acacagtgta atcgataaca aatgatactg tcaaagctca aaaacgtatg taagcccttg
     1681 cggttctatg gtcttcactg gaagtaaacg cttgacctct tcaagttctt ttttgcactc
     1741 gttatttaac ataaagccct gattgtacgc accgctaaaa cggcctcagg gcgtttccga
     1801 cgggaaaccg gtaaaacagg caacaaacca cccgaaaacc cgccagaaac gcgcaggctc
     1861 tgtttttacg gcatgcatga gtatgcacgc aagtgcatag cgcgaaaatt cacgccattc
     1921 agccagggta agtcctgttt ccggcgctct tcctatccga aaacggtgaa cggggacgca
     1981 atttattgcg tggccgtgaa gcgttgccgg cgcagccgga aaaccggttt tcagcacgat
     2041 caggaatcgg gttcgtcgac ggccggcaat cacgttatgg caagtcgccg gacgactcac
     2101 cacacgattc accgacgatt cgactcgtga cttgactcta gagtcgtcaa ggtcaaaaaa
     2161 acaccaaaat gacgattcga ctctagagtc aagtcgtcat aagttactga tagtcttaag
     2221 tgagtcgtcg atgactctat gacgagtcgt cggcaagtcg tacacgaatc gccgacgact
     2281 tggattcacc ttacagaaag taatactgcg taatgtaata acccacaact agctgatatt
     2341 aatgagttat cttcaaaatt ttaattttgc gtggggtgtg ggtattttta gtggtgagga
     2401 gcgttcagag atctgacgat ttttgggaag gttcaggaac tcctcggaat ggccggagag
     2461 gaaaactgaa gcatgacggg gtcaggattt tttccagaat ttccaccatg gcgccgggga
     2521 ttcagttaat tttgcctggt gattttcaag aaggagcatg gcctgtttca ggctgtcaat
     2581 gtgcgaatcc ttggacaaaa gtaaggtgtt caggtgttca atctcctgtt tcagatgacg
     2641 aatcacctgt tcactctggt cagcagaggc tgtatcacgc tgttcagaga cctgtccaga
     2701 atggtgttca cccttcggtt gctcaaaggg accaaagaca cgcaaaagct ctgaagtatc
     2761 taccattttt gcattgcgct caccactaca ggtggacagg tcacctgttt taattaacct
     2821 gtgcagtgtt gttctactct tacccgtcag tcgggcagcc tcactaatag acactctggc
     2881 catcagttac catcctgtaa tgcatccagt ctcgaggaca gtgtacggaa aagatgctcc
     2941 gcatcatcgt ggagtttctc acaaaggtcg gcctcgacat cgagatcaag ctcgttaagc
     3001 ttctccagca gaagcagaga ctgattctgg atgaatgtag ccatgttcag cgctgttttc
     3061 tgttgtttcg tcataagagg cctcagagcg tcagtgctca gtg
//
