LOCUS       NZ_CP095174             4918 bp    DNA     circular CON 21-MAY-2024
DEFINITION  Enterobacter roggenkampii strain K666 plasmid pK666_4k, complete
            sequence.
ACCESSION   NZ_CP095174
VERSION     NZ_CP095174.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27363726
            Assembly: GCF_023375585.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Yin,Y.
  TITLE     Potential Transmission of mcr-10-Harboring Enterobacter spp. among
            Companion Animals and Humans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Yin,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2022) Baisc Veterinary, Yangzhou University, No.
            48, wenhui east road, hanjiang district, Yangzhou, Jiangsu 225000,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023375585.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 04:40:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,080
            CDSs (total)                      :: 4,962
            Genes (coding)                    :: 4,851
            CDSs (with protein)               :: 4,851
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 52 of 111
            Pseudo Genes (incomplete)         :: 48 of 111
            Pseudo Genes (internal stop)      :: 27 of 111
            Pseudo Genes (multiple problems)  :: 13 of 111
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095174.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="K666"
                     /host="healthy cat"
                     /db_xref="taxon:1812935"
                     /plasmid="pK666_4k"
                     /geo_loc_name="China: Jilin"
                     /collection_date="2019"
     gene            280..612
                     /locus_tag="MUY33_RS25300"
                     /old_locus_tag="MUY33_25300"
     CDS             280..612
                     /locus_tag="MUY33_RS25300"
                     /old_locus_tag="MUY33_25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_064401063.1"
                     /translation="MSHAIEFIETTMFTRQIKQIATDDELKELQKELIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSITDAEKAELKKLTQ
                     RLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            614..898
                     /gene="nadS"
                     /locus_tag="MUY33_RS25305"
                     /old_locus_tag="MUY33_25305"
     CDS             614..898
                     /gene="nadS"
                     /locus_tag="MUY33_RS25305"
                     /old_locus_tag="MUY33_25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_064401064.1"
                     /translation="MSFFDELKASLEEAVEIKNGTKAPARVTRYELADVRAIRENLNIT
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFHELAAH"
                     /name="nadS"
     gene            1094..1990
                     /locus_tag="MUY33_RS25310"
                     /old_locus_tag="MUY33_25310"
     CDS             1094..1990
                     /locus_tag="MUY33_RS25310"
                     /old_locus_tag="MUY33_25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_064401065.1"
                     /translation="MRNAALELFNDRLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRIGAAIDWSDRNAPAPTLTITNPENGHAHLLYALKTSIRTAPDGKMK
                     PLRYAAAVENALRKKLGADTGYSGLICKNPNHSHWKIAVWQPELYTLDWLADSLDLNAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEFDQWLQACYERASAYNLQFAAPLD
                     ENEVKWISKSVARWTHRKITKESFSDYVSRTHTSELQSFRGRKSHGGGRNKNPHSEREI
                     KPWDLLGISRATYYRRK"
                     /name="replication initiation protein"
     gene            complement(1999..2964)
                     /locus_tag="MUY33_RS25315"
                     /old_locus_tag="MUY33_25315"
     CDS             complement(1999..2964)
                     /locus_tag="MUY33_RS25315"
                     /old_locus_tag="MUY33_25315"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_086538494.1"
                     /translation="MWWSKIQIGIDLATSVTIFAAALTWWMNKVRERRVGVIDSARSTI
                     IENINNATNEMALSFNDFATFTTSIERKINTKLRFGEDKFIDEIASGRIDVVEIHTLFS
                     KSVDTMGVFYERGATLRYTIFPSLYSIGGEDEAVSILKKEINDVLVAYNKSQSGYIAYI
                     KEITSICEKIKKIKASDPSADLVSIIQDEYIGSIFSIVDDPDYGIFLQDFLDDGDDDLF
                     LLSLHKKSEEMTDKEKELRLNVAKFLIYMIANKPESLISQFLQRLAFRMQENRVQCKEF
                     LVTLSAISSKMQQRSSDFSIKKAYDVLSSEKYFNTKEEIR"
                     /name="hypothetical protein"
     gene            3267..3593
                     /gene="mobC"
                     /locus_tag="MUY33_RS25320"
                     /old_locus_tag="MUY33_25320"
     CDS             3267..3593
                     /gene="mobC"
                     /locus_tag="MUY33_RS25320"
                     /old_locus_tag="MUY33_25320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_064401068.1"
                     /translation="MEKRTKEIKIRMTETEHQRLLERCDRLHLAEWLRQLGLGEQTSRK
                     RPVPEVAPELLRQISGMGNNLNQIARRLNQSDSLTPSERVSLLSLLNSLDRQLGELLEQ
                     HRDR"
                     /name="mobC"
     gene            3802..>4224
                     /locus_tag="MUY33_RS25325"
                     /old_locus_tag="MUY33_25325"
                     /pseudo=""
     CDS             3802..>4224
                     /locus_tag="MUY33_RS25325"
                     /old_locus_tag="MUY33_25325"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4271..4756
                     /locus_tag="MUY33_RS25330"
                     /old_locus_tag="MUY33_25330"
     CDS             4271..4756
                     /locus_tag="MUY33_RS25330"
                     /old_locus_tag="MUY33_25330"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_064401061.1"
                     /translation="MSEILNLAKGFEEKSKQQAQDTAQSVKREFEKLETSICEDLKSSS
                     QSISTAIREQNDQLTGIVRYSLLSALGWIFLFAALLIMILGGIIWYQGTVITSRLDEMD
                     SYSQQLESLKAQSGAGVAIYKDEKQKNTYMVVLPKKAHGTETYTSTSGNTVLKYTAK"
                     /name="MbeB family mobilization protein"
     Typing          3584..4875
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="80.974"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 cgcatgttcg agactacctc acaggcgctg gagcgcaacg acagggtatg tctgaacttg
       61 agcaatcagc tgaacggttt gtacgagcag gtcgaacgct tgagcagcga tctgagccga
      121 ttaacacgct cgtaaagcag caggaacggc agttacagca ggagaagcaa gcaaccccct
      181 cacgaggttg gtctaggtaa cgtctgatgg tacagaaagc ggctttcctg agcgtggttg
      241 ttggtgtaca ttgtactcta aaatatacag gagacagccg tgagtcacgc gattgagttt
      301 atcgagacaa cgatgtttac ccgtcagata aagcaaatcg ccacggatga tgaactcaaa
      361 gagctccaga aagagcttat cgagtcaccg gacaaaggtg acctcatcca gaaaacgggc
      421 ggtctgcgaa agatcaggat ggcgacgggg aatcagggaa aaagcggcag tgcgcgggtg
      481 atttacttcc ttgccaccgc tgaagtgatt tatctggtga tggcctaccc taaaagcacc
      541 aaagacagca tcacagacgc agagaaagcc gaactgaaga agctgaccca acgactgaaa
      601 gacgaggtgt aacatgagtt tttttgacga gctgaaagcc tctctggaag aggccgttga
      661 aatcaaaaac ggaaccaaag cccccgcccg tgtgacccgc tacgagctgg cagacgtcag
      721 ggctatcagg gaaaacctga atattactca gagcgaaatg gcgaaagtgc tgggaaccag
      781 cctcgacacc atcaagagct gggaaaccgg acgccgtaac ccgaccggac tggccgcaaa
      841 agtgctggcc accattcagg ctaacccgaa attctttcat gaactggcgg cgcattaagc
      901 tgatgtgaca gaagaaattc gacaggcgag gccgttcgcg tgtaacttta acggcagcag
      961 aaaaaacgaa gccccggaaa tcatgagcta tcttggcgga tgagcatgat taccagggcc
     1021 aggtacgaaa ccttgaaagg atattagcac atgaaccgtg ccagacaaca gcccgccata
     1081 agcgcagggc acattgcgta acgcagcact cgaacttttc aacgaccgct taccgcacaa
     1141 accgtacttc tcggatgatt tgcactttgg tgttcgcatc gcagggaaag aacgcgccat
     1201 tctggcgaaa tacatccagt tcaaccagcc tcacgccatg ttctggctgg gatttgacgt
     1261 tgaccgcatc ggcgcagcta tcgactggag cgaccgcaac gctcccgcgc ctacactgac
     1321 catcaccaat ccagaaaacg gacatgcgca cctgctttac gcgctgaaaa cctcgatacg
     1381 caccgcgcca gacggaaaaa tgaaaccgtt acgctatgca gctgctgtag agaacgctct
     1441 acgtaaaaaa ttgggcgccg acacgggcta ttcggggcta atttgcaaga atccgaacca
     1501 cagtcactgg aaaattgccg tctggcagcc tgagctctac acgctggact ggttagctga
     1561 ctcactcgac ctgaacgccg cgaacgacaa agaaattgtc gctgattatg gcttaggccg
     1621 taactgcacc ctgttcgaca aaacccgcaa gtgggcatat cgtgccatcc gtcagggctg
     1681 gcctgagttc gaccagtggc tgcaagcctg ttacgaacgt gccagcgcct ataatctgca
     1741 atttgcagct cctttagatg aaaatgaggt taaatggatt tcgaaaagtg ttgccagatg
     1801 gactcataga aaaatcacaa aggaatcatt ttctgattat gtgtccagaa cacatacgtc
     1861 agaactacaa tctttcagag ggagaaaaag ccatggcggg gggcggaata aaaatcctca
     1921 ctcagaaaga gaaattaagc catgggatct gttagggata agcagggcta cttactacag
     1981 gcgtaagtag cctttaaatt atcttatttc ttcttttgtg ttgaaatatt tttcagaact
     2041 taacacgtca tatgcttttt ttatggagaa gtcactggaa cgttgctgca ttttacttga
     2101 gatagcactt agtgttacaa gaaactcctt acattgcact cggttttctt gcattcgaaa
     2161 agccagcctt tgcaggaatt gagatatcaa agattcaggt ttgtttgcaa tcatatatat
     2221 taagaatttc gccacgttca atcttaattc tttttctttg tcggtcattt cttccgattt
     2281 tttatgtaat gataataaaa acaaatcatc atcaccatca tctaagaaat cttgtaaaaa
     2341 gatgccatag tcaggatcgt caacaatact aaatatagat ccaatgtatt cgtcttgaat
     2401 gatcgacacc aagtctgcgc ttggatcgct ggcttttatt ttttttattt tttcacatat
     2461 ggatgttatc tcttttatgt atgcaatata cccggattga cttttattat acgcgactaa
     2521 gacatcattt atttcttttt ttagaataga taccgcctca tcttcccccc caattgagta
     2581 taatgatggg aagattgtat atcttaatgt tgcccccctt tcataaaaca ctcccattgt
     2641 gtctacgctt tttgagaaaa gtgtgtgtat ttcaaccaca tctattctcc ctgatgctat
     2701 ttcatcaatg aatttatctt ctccaaatcg taatttggtg tttatttttc gctcgatcga
     2761 tgttgtgaat gtggcaaaat cattaaaaga taatgccatt tcatttgtcg cattgttgat
     2821 attttctatt attgtggatc gagcactgtc aataacgccc actctcctct ccctcacctt
     2881 gttcatccac caggttaagg ctgccgcaaa gatagttaca cttgtcgcaa ggtcgatgcc
     2941 aatctgaatc ttactccacc acataaacaa ttttccctta tagttagcta ccgcagattc
     3001 aaaaaatacc caaagctaaa aactaatcaa cacgatttga gaccaaaaca acctatatca
     3061 gataacagcc tcttttgcag ggtccgtttt tttgcagctt ccatagaatc tggcatactg
     3121 aaaataccct ttaagccagt acacacagcg tcccctctac gaggttccgt ctgtgactgg
     3181 ttcagggggc tcgccgcccc ccgaaacccc cggcatccac tgcgaaaaat tctcacagtg
     3241 gtgcgaattt tttctcagcg gagcctatgg aaaagcgaac gaaagagatc aaaatccgta
     3301 tgaccgagac tgagcatcag cgtctgcttg aacggtgcga ccgtcttcac cttgcagagt
     3361 ggttgcgtca gcttggtctg ggagaacaga catcacgtaa acggcctgtt cctgaggttg
     3421 cgcccgaact gcttcgacag atcagtggta tgggcaataa cctgaaccag atcgctcgtc
     3481 gactgaatca gtccgattcc ctgacgcctt ctgagcgtgt ctcactgctt tccctcctga
     3541 acagcctcga ccgtcagcta ggggagttac tggagcagca ccgtgatcgt taaaatccat
     3601 tcccgtggtg caggttctgg cagtggtccg gttgattacc tgctgggaaa agaccgtcag
     3661 cgagagcagg ccaccgtatt acggggtgat cctgaccgtg tatgcgaact gatagacagc
     3721 tgcgactttg cccgttcgta tacctctggc gtgctgtctt ttcaggagcc ggatattgcc
     3781 gactctgaga aatcccgcct gatggacgag tgggaacaga ccctgatgac gggattagac
     3841 cgtgaccagt acaactgtct ctgggtagag caccgcgaca agggacgact tgagctgaat
     3901 tttgtcatcc cgaacatcga attgcagagc ggaaaacggc tacaacccta ctttgaccgc
     3961 gccgaccgcc ccagagtgaa tgcgtggcag accctgacca atgaccgcct cggattacgt
     4021 gatcctaatg acccccttaa ccgccgtccg atgacgcagg ccagcgactt accccgcgac
     4081 aaacagcagg ccgcagagaa aatcaccgcc ggtctgatga acctgatgca gcagggtgag
     4141 atccgcagcc ggcaggatgt ggttaagcag ctggagagct acggcctgac tgtggcgcgg
     4201 gaaaccaaaa acagtatcag cattgccgat ccggacggcg ggaaaaatat ccgacttaaa
     4261 ggaatgattt atgagcgaga ttttaaattt ggcgaagggc ttcgaggaga aatcgaagca
     4321 gcaggcgcag gataccgcgc agagcgtgaa gcgcgagttc gagaagctgg aaacgtctat
     4381 ctgcgaggac ttgaaatcaa gctcgcagag catcagcacc gctatccgcg aacagaacga
     4441 ccaactgacg ggcatagtca gatattccct cctgagcgca ctgggctgga tattcctgtt
     4501 cgccgcgctg ctgatcatga tactgggcgg gataatctgg tatcagggaa cggtcatcac
     4561 ttcccgcctg gacgaaatgg acagctacag ccagcaactg gagagcctga aagcccagag
     4621 cggcgcgggg gtagcgattt acaaggacga aaagcagaag aacacctaca tggtggtact
     4681 gccgaagaaa gcgcatggca cagagacata cacatcgaca tccggaaaca ccgtgttgaa
     4741 gtacaccgcg aaataacccc atcagacacc acagaaacgc cacagcacgt ttttatcact
     4801 gaggacaggc aaaaccatga ccgaaatgga aaggcagctt ctgagcgcct tagaaagctt
     4861 acagacaagc tacgaacaac agcagcaggc gtggcaggac agttacagca acttgcaa
//
