LOCUS       NZ_CP095173             5966 bp    DNA     circular CON 21-MAY-2024
DEFINITION  Enterobacter roggenkampii strain K666 plasmid pK666_5k, complete
            sequence.
ACCESSION   NZ_CP095173
VERSION     NZ_CP095173.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27363726
            Assembly: GCF_023375585.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Yin,Y.
  TITLE     Potential Transmission of mcr-10-Harboring Enterobacter spp. among
            Companion Animals and Humans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   Yin,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2022) Baisc Veterinary, Yangzhou University, No.
            48, wenhui east road, hanjiang district, Yangzhou, Jiangsu 225000,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023375585.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 04:40:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,080
            CDSs (total)                      :: 4,962
            Genes (coding)                    :: 4,851
            CDSs (with protein)               :: 4,851
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 52 of 111
            Pseudo Genes (incomplete)         :: 48 of 111
            Pseudo Genes (internal stop)      :: 27 of 111
            Pseudo Genes (multiple problems)  :: 13 of 111
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095173.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="K666"
                     /host="healthy cat"
                     /db_xref="taxon:1812935"
                     /plasmid="pK666_5k"
                     /geo_loc_name="China: Jilin"
                     /collection_date="2019"
     gene            complement(join(4809..5966,1..345))
                     /locus_tag="MUY33_RS25460"
     CDS             complement(join(4809..5966,1..345))
                     /locus_tag="MUY33_RS25460"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_048702755.1"
                     /translation="MIVKFHPRGRGGGAGPVDYLLGKDRQREGASVLQGKPEEVRELID
                     ASPYVKKYTSGVLSFAEADLPPGQREKLMASFERVLMPGLDKDQYSILWVEHADKGRLE
                     LNFLIPNTELLTGRRLQPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSGL
                     PKTKQEAAEAITRGLLSLASSGELKSRQDVTDTLEGAGFEVVRTTKSSISIADPDGGRN
                     IRLKGAIYEQSFYGLEGLRGEIESAAAEYRRDAEGRIQRARAVCRSGTERKREENQRRH
                     QRPRAEYERGDGEKPAERAADGREDMAYHPDGFSAADRHEREHSVVAGAADNRQLHDPS
                     PAGGRPGEDERENMGCELPERQQRPVPGAAEGSESRNRLDSGQREAERGEAGTGVEQND
                     RAGKAVAERIRAATAGLLAKAGRMGERLRGMAEHVWAYATGERGAERARVGLEHAGAEF
                     KRAAAPLVRELNAIELRQRENEYQKTLELERQPQKTYRGPTL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(335..658)
                     /locus_tag="MUY33_RS25265"
                     /old_locus_tag="MUY33_25265"
     CDS             complement(335..658)
                     /locus_tag="MUY33_RS25265"
                     /old_locus_tag="MUY33_25265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_029593203.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1307..1465
                     /locus_tag="MUY33_RS25465"
     CDS             1307..1465
                     /locus_tag="MUY33_RS25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            1822..2421
                     /locus_tag="MUY33_RS25270"
                     /old_locus_tag="MUY33_25270"
     CDS             1822..2421
                     /locus_tag="MUY33_RS25270"
                     /old_locus_tag="MUY33_25270"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049002646.1"
                     /translation="MKLINSFALSCFFFSISALAAQELVINNSALHIIFADGTEANVSS
                     CSDFISLNQAGMKIKELPDLSDPDYQQAHMELSKCLMHSWAIQNGYTQKTSSAITLDEV
                     VKHFPATADLSPASGYKTMLQTIPDLKPKDDRFVSHTKEEGYVINDAWTFKNSSGQEAD
                     FVMLVGYATGGTMAVARLWRIDSKTTTPWGVTEIKP"
                     /name="hypothetical protein"
     gene            complement(2448..3230)
                     /locus_tag="MUY33_RS25275"
                     /old_locus_tag="MUY33_25275"
     CDS             complement(2448..3230)
                     /locus_tag="MUY33_RS25275"
                     /old_locus_tag="MUY33_25275"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224753151.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVT
                     VGIGRMLPNSQAGYNLGFTKTHHIPRGHGDSEDVEISASHGDIDSAFNATKEKRASNVH
                     LSDQSMVADCVNQLDKDRTTLRNMYTDFDHFPQPAKTALHDMLYNLGEGKLHSEFPNFN
                     AAVNRKDWTRAATESHRTGIGDSRNKDTHDQFIAAGNK"
                     /name="hypothetical protein"
     gene            3448..3888
                     /locus_tag="MUY33_RS25280"
                     /old_locus_tag="MUY33_25280"
     CDS             3448..3888
                     /locus_tag="MUY33_RS25280"
                     /old_locus_tag="MUY33_25280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(3962..4366)
                     /locus_tag="MUY33_RS25285"
                     /old_locus_tag="MUY33_25285"
     CDS             complement(3962..4366)
                     /locus_tag="MUY33_RS25285"
                     /old_locus_tag="MUY33_25285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="WP_008321396.1"
                     /translation="MTILVTIQAQLVVGEEQVIKSLAPEGMLAAVFEDDGRTGYFYALD
                     ESVEGNPIMDAVHIYNAEDVSDGHIPSDVKIGWSEDSQKCVLLINGYPHGAFDFVGKNG
                     YCRSGYPPPINKVWSLSGHEWSDSVDDFFR"
                     /name="DUF2251 domain-containing protein"
     Typing          complement(772..945)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          869..1474
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2..345)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.13"
                     /coverage="87.0"
                     /evalue="4.859999999999996e-69"
     Typing          complement(342..658)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.34"
                     /coverage="99.0"
                     /evalue="1.7899999999999997e-61"
ORIGIN
        1 cagcagctcg gtattgggga tcaggaagtt cagctccaga cgccccttgt ccgcgtgctc
       61 cacccacaga atgctgtact gatctttatc gagtccgggc atcagaaccc gctcgaagct
      121 ggccatcagt ttttcccgct ggccgggagg cagatcggcc tccgcaaacg acaggacgcc
      181 ggaggtgtat ttcttcacgt aaggcgacgc gtcaatcagc tcccggacct cttccggctt
      241 cccctgcagg acgctggcgc cttcacgctg ccggtcttta cccagcagat agtccactgg
      301 accggcgcca ccgccgcgac cccgaggatg aaatttaacg atcatcgtcc gttcctttct
      361 caagaacggc gtgccgcagc cgctcaagtc cggcatcaat ggccatcagc gccgccacaa
      421 tctgcaccct gtcatgaccg gtaccgccgc cggcgttaac gcggcgggct atctggttca
      481 ggttgttgcc catgccagca agctggcgca gcagcgccgg cgagagtgag gggagctttc
      541 cggtgcgcgc aggcttttcg tccaggcagg tctgcctcat ccacgctgcc agctgcctgc
      601 cgtcgcagcg ctcgagcagc cgccggtgct cgtcctccgt cacccacatg gtgagcatct
      661 tgttccgttt ctctgacaaa ccggcctcct gataacgccg ggacggacgg gaaaggcttt
      721 cccggtcggc gcggggttgg acaagccgca ggcgcgtcag gcttttggcg ggtttcgggg
      781 cgcagccctg aaccagtcac gtagcgctag cggagtgtat actggcttac tatgttgcca
      841 cgattgcgaa aatcagtgaa gtgcttcatg tggcaggaga aaaaaggcag caccggcgcg
      901 tcagcagaat atgcgataca ggagatattc cgcttcctcg ctcactgact cgctacgctc
      961 ggtcgttcgg ctgcggcgag cggtaccggc ttacggacgg ggacggaaat ttcctggaag
     1021 ataccaggaa gacacttagc aaagaagaga taaggccacg gcgaagccgt ttttccatag
     1081 gctccgcccc cctgacaagc atcgcaaaat ctgacgctca aatcagtggt ggcgaaaccc
     1141 gacaggacta taaagatccc aggcgtttcc ccctggtagc tccctcgtgc gctctcctgt
     1201 tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcggtttc tcattccacg
     1261 cctgacactc agttccgtga aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     1321 cgttcagtcc gaccgctgcg ccttgtccgg taactatcaa cttgagtcca acccggaaag
     1381 acacgacaaa acgccactgg cagcagccat tagtaacagg agtgcacgag aagatagatt
     1441 aagaggtctt gaagtagagg cctgactgcg gctacactga aagaacagtt ttggtaatgg
     1501 cggtctcgtg aaccctgtta ccgggttaag cagttcccca actgacttaa ccttcgaaca
     1561 aaccgcctcc ccaggcggtt ttttcgttta cagagcaaga gattacgatt cattgaaaag
     1621 gatctcaaga agatcctttg tagattacta tcaattaatc ttccgaacat gacaccagtc
     1681 actgttgatt acggttaaac cgctccgtaa caggtttccc tcgtattggt gacttgcagc
     1741 accaaagaag caaaaaagat aaatatatct acttattgag agatgatata tttatttcca
     1801 aaatgctaca gatacatcat catgaaacta attaactcat tcgccctgtc atgtttcttc
     1861 ttttcaatta gcgccctcgc cgcacaagag ctggtaatta acaattcagc cctacacatt
     1921 atctttgctg acggcacaga ggctaatgta tcctcatgca gtgactttat ctctctaaat
     1981 caagcaggta tgaagattaa ggagctaccg gatctgtcag accctgatta ccagcaggcg
     2041 catatggagc tcagcaaatg cctaatgcat tcttgggcta tccagaacgg atatacccaa
     2101 aaaaccagtt cggcaatcac acttgatgag gtagtgaagc acttccctgc gacagcagac
     2161 ctgagcccag cctctggtta taaaactatg ctgcaaacaa taccggactt aaaaccgaag
     2221 gatgaccgct ttgtgtcaca taccaaagag gaagggtatg tgattaatga tgcttggaca
     2281 tttaagaact catccggcca agaggcagat ttcgtgatgt tggtgggcta tgcaaccgga
     2341 ggtacaatgg ctgttgcccg tctctggcga atagattcaa aaaccaccac cccctggggt
     2401 gttaccgaaa taaagccata aaaaaagccg cattatgcgg ctttttctta tttattacct
     2461 gcagcaataa actggtcgtg tgtgtctttg ttacgggaat caccgatgcc agtgcgatgg
     2521 ctttcagtcg cagctctggt ccagtcttta cgatttactg ccgcattgaa attagggaat
     2581 tcagagtgca atttcccttc tcccaaatta tacagcatat catgcaatgc agtcttggct
     2641 ggttgcggga agtggtcgaa gtcagtatac atgttgcgca gggtggtacg atccttgtcc
     2701 agttgattca cgcaatccgc aaccattgac tgatctgaga gatggacatt ggatgccctt
     2761 ttctctttgg ttgcgttaaa cgcgctatct atatcaccat ggcttgcgct tatttctaca
     2821 tcttctgaat ctccatgacc acgcggaata tggtgtgtct tagtaaatcc cagattatag
     2881 cctgcttgcg aattcgggag catgcgacca atccccactg ttacattgtt cgcagtatct
     2941 aggtacatgt tattgcgtgc accttcatga tgtataatgt tattaatgac agcagaaaga
     3001 tcgacagaat taatattctt cgtacctggg acagttatcg ggtgtcggcc agtgatgaga
     3061 ccaaaggatg cagtataaga tgaaccacta ctattatcat ggccaccatt accaccaccg
     3121 ttaaaactac ctcccgaact tccagagccg ctatgaccat cattgtaggg gtcatttggt
     3181 cccatgtgac caaaaccacc gacagtaccg ctatttaact ctgtactcat aaaattatcc
     3241 tctttgactt ttaaaaacag caaaaactgt atttataaca atgtgtttat atacagtatt
     3301 cgaaatacta cgcccgtcaa aaccacaccg tcaacaggtt ttttgtgtat ttttcacgcc
     3361 tcacgggcag cctcagacct tttgtgttaa aacagtaata tgttctgctg ttacaatacg
     3421 gtacacacag gagacactgt cacttttatg gcatggcaca cagttcaaga gacactgaaa
     3481 ttaacaggaa agtcgcgctc gcaattatat cgtgacatgg ctaaaggcct tgtcagctat
     3541 cgcacaggaa aaaatgatcg ccgggagttc gagaccagtg aactgattcg ggcctatggc
     3601 gagctgcgca cttctgagac accaaaaagg cacaaggagg gacactccaa atctgtcgtt
     3661 agccaggcgg aaaatgctca gctagacgtc attcagcaga aactggacgc tctgcagcag
     3721 actgttaccc taatgcttga agacaagatg gcgcgggaag cagaaaaccg cctgcgaaat
     3781 gaggagaggg aacaactaca ggccgaaatt agccgtctgg ctgacgcgct ggcacaggaa
     3841 aaaaagcggg gattctggtc gcggctgttt cgctccggtt cagagtaaca gtgcaggtga
     3901 ctctgattta ccataacatc cgttgttcga accataatgg caggctatag cctgccgtaa
     3961 ttcaccggaa aaaatcatcc accgagtcgc tccactcatg cccggaaaga gaccacacct
     4021 tgtttatagg gggaggataa ccactcctgc agtacccgtt ttttcccacg aaatcgaatg
     4081 ccccgtgcgg gtatccattg atcaggagta cgcacttctg actgtcttct gaccagccaa
     4141 ttttaacatc agaaggaatg tgaccgtctg aaacgtcttc ggcattgtag atgtgtactg
     4201 cgtccataat cgggtttccc tcgacggact catcgagcgc atagaaatac ccggtccggc
     4261 catcgtcttc gaacacggcg gccagcatgc cctcaggagc gaggctcttt ataacctgtt
     4321 cctccccgac aacgagctgg gcctgtatcg tcactaaaat ggtcatcagg tctgttctcc
     4381 tttccttggt tgcttcggca gcccgctttc agacaagcgg ataatttacc ataacatctg
     4441 ttgggcgcac cgctgaaacg gggttggctg cgtttccggt gccagacgcg agaaagtggc
     4501 gacagacata acgaaaaaac accagaaacg cagccgctga atattacact ggtgcatagt
     4561 catgcagcca gatgcatgga tgttaaagtc acactaaaat ccgcgccaat gccttaaaaa
     4621 ccgacaaatg cttctccagg cagtcccaca gctccgccgc cagatacagg cagttaagca
     4681 atgattcaat aagatttaac agcttcattt cccgctcctg aataagtcgt gacctttttg
     4741 atcacgaacc tatttagcgg gagaaataag agcccctctt cgtgcgggtt gtcagctgcc
     4801 tggatggttt aaagtgtcgg cccgcggtag gttttctgcg gctggcgctc cagctccagt
     4861 gttttctgat actcattctc cctctgccgg agctcgatgg cattcagttc ccggacaagc
     4921 ggcgcagctg ctcgcttaaa ctcagcacct gcgtgctcaa gtccgacacg cgctcgttca
     4981 gcaccgcgtt ctcccgttgc ataagcccac acatgctccg ccattccgcg aaggcgctct
     5041 cccattcgtc cagcctttgc gagtagtcct gctgtagctg ctctaatgcg ctcagcaact
     5101 gcttttccag ctctgtcatt ctgctctact cctgtaccag cttcaccgcg ttccgcttcc
     5161 cgctgtccac tgtccagcct gtttcggctt tcgctccctt cggcagcacc aggaaccggc
     5221 cgttgctgtc gctctggtag ctcacacccc atgttttcgc gctcatcttc gccagggcgt
     5281 cctcctgctg gcgaagggtc gtgtaattgt cggttatctg ctgcccctgc caccacagaa
     5341 tgctcccgct cgtgccgatc agcagcgctg aaaccatcag gatggtaagc catgtcctcc
     5401 ctgccgtcag cagcacgctc cgccggtttt tctccatcgc ctcgctcata ctctgctcgt
     5461 gggcgctgat ggcgtcgctg attctcctcg cgcttgcgct cagttccgct ccgacagact
     5521 gctcgtgctc gctgaatgcg gccttcagca tctcgccggt actctgctgc tgcgctttca
     5581 atttctcctc gaagtccctc gaggccgtaa aaagattgct catagatggc tcccttcagt
     5641 cggatgtttc gccccccgtc cgggtcggcg atgctgatac tgcttttcgt ggtgcgcacc
     5701 acctcaaaac ctgcaccttc aagagtgtca gtgacgtcct gacggctttt cagctcccct
     5761 gacgaggcta agctcagcag cccccgggta atggcttctg cggcctcctg cttcgttttc
     5821 ggcaggccgg acggcgtgac cagcgcccgg cggttttccg gcgcgttcgg gtcgtgcagc
     5881 cccagtctgc cgttcacgat ggtctgccag gcatcgatgc gcggccggtc ggctcggtcg
     5941 tagtatggct ggagccgtct gccggt
//
