LOCUS       NZ_CP095168             4783 bp    DNA     circular CON 21-MAY-2024
DEFINITION  Enterobacter roggenkampii strain K475-2 plasmid pK475-2_4k, complete
            sequence.
ACCESSION   NZ_CP095168
VERSION     NZ_CP095168.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27363725
            Assembly: GCF_023375565.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Yin,Y.
  TITLE     Potential Transmission of mcr-10-Harboring Enterobacter spp. among
            Companion Animals and Humans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Yin,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2022) Baisc Veterinary, Yangzhou University, No.
            48, wenhui east road, hanjiang district, Yangzhou, Jiangsu 225000,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023375565.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 04:29:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,997
            CDSs (total)                      :: 4,879
            Genes (coding)                    :: 4,763
            CDSs (with protein)               :: 4,763
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 45 of 116
            Pseudo Genes (incomplete)         :: 79 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095168.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="K475-2"
                     /host="healthy cat"
                     /db_xref="taxon:1812935"
                     /plasmid="pK475-2_4k"
                     /geo_loc_name="China: Jinlin"
                     /collection_date="2019"
                     /collected_by="Jilin university"
     gene            join(4700..4783,1..132)
                     /locus_tag="MUY30_RS24900"
                     /old_locus_tag="MUY30_24900"
     CDS             join(4700..4783,1..132)
                     /locus_tag="MUY30_RS24900"
                     /old_locus_tag="MUY30_24900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(803..1045)
                     /locus_tag="MUY30_RS24905"
                     /old_locus_tag="MUY30_24905"
     CDS             complement(803..1045)
                     /locus_tag="MUY30_RS24905"
                     /old_locus_tag="MUY30_24905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            1232..1549
                     /locus_tag="MUY30_RS24910"
                     /old_locus_tag="MUY30_24910"
     CDS             1232..1549
                     /locus_tag="MUY30_RS24910"
                     /old_locus_tag="MUY30_24910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1752..2108
                     /locus_tag="MUY30_RS24915"
                     /old_locus_tag="MUY30_24915"
     CDS             1752..2108
                     /locus_tag="MUY30_RS24915"
                     /old_locus_tag="MUY30_24915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(<2428..2550)
                     /locus_tag="MUY30_RS25015"
                     /pseudo=""
     CDS             complement(<2428..2550)
                     /locus_tag="MUY30_RS25015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            3201..3524
                     /locus_tag="MUY30_RS24920"
                     /old_locus_tag="MUY30_24920"
     CDS             3201..3524
                     /locus_tag="MUY30_RS24920"
                     /old_locus_tag="MUY30_24920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_163063187.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3733..>4167
                     /locus_tag="MUY30_RS24925"
                     /old_locus_tag="MUY30_24925"
                     /pseudo=""
     CDS             3733..>4167
                     /locus_tag="MUY30_RS24925"
                     /old_locus_tag="MUY30_24925"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4202..4693
                     /locus_tag="MUY30_RS24930"
                     /old_locus_tag="MUY30_24930"
     CDS             4202..4693
                     /locus_tag="MUY30_RS24930"
                     /old_locus_tag="MUY30_24930"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032629998.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     Typing          2913..3086
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          complement(2438..2946)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          3202..3518
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.45e-59"
     Typing          3515..4782
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.647"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gccttcgcgg aatggcggag catgtgtggg cttatgcaac gggagaacgc ggtgctgaac
       61 gagcgcgtgt cggacttgag cacgcaggtg ctgagtttaa gcgagcagct gcgccgcttg
      121 tccgggaact gaatgccatc gagctccggc agagggagaa tgagtatcag aaaacactgg
      181 agctggagcg ccagccgcag aaaacctacc gcgggccgac actttaaacc atccaggcag
      241 ctgacaaccc gcacgaagag gggctcttat ttctcccgct aaataggttc gtgatcaaaa
      301 aggtcacgac ttattcagga gcgggaaatg aagctgttaa atctcattga atcattgctt
      361 aactgcctgt atctggcggc ggagctgtgg gactggctgg agaagcattt gtcggttttt
      421 aaggcattgg cgcggatttt agtgtgactt taacatccat gcatctggct gcatgactat
      481 gcaccagtgt aatattcagc ggctgcgttt ctggtgtttt ttcgttatgt ctgtcgccac
      541 tttctcgcgt ctggcaccgg aaacgcagcc aaccccgttt cagcggtgcg cccaacggat
      601 gttatggtaa atcagtatca gctgcagata taaaaaagcc gccaggctgg cggcttgctg
      661 ttattttcgt ggctttcctg catctgagcg ttttcctctg acccttccgt cattgttacg
      721 gtcacgaggt tgagttccgc cgcctggttt aggaacgaca acgtgtgggt tgagtggtcg
      781 acgatctgcc attttctata cctcactgag cataaacaaa caattgccaa cgaatgttcc
      841 cttgcgcggg ggtatgttgg tggtggtttt gtggtgggat tgttttcccg cctgcgatag
      901 ttacttcttt accgcaaccg gtacatttat aaatcccttc atattcacat gtttctcccg
      961 gcttatattc agtgtcaaaa accgagttaa acttcgtatc tgcttcaaaa atcttgcttt
     1021 tgtttcctga gcgaacaaga gccatgcaac ctccaaatta tcatttattt tgcacacctg
     1081 cactgtgcat gagtgcattc tacagcatag ccattgattg cacaagtgca ctgtgctgtt
     1141 gtgcatttga tatgtttgct gtactatatc ggaggtaaga aggtgatcga aaaaattgcc
     1201 aagttgcggc aaaaattgag gtaaaagaaa aatgcagcaa gatcatgaac atgatgattg
     1261 ccgcattcag tttcgagacg attggctata ccagttttac aaatatggta aagccaatcg
     1321 aaagattcca actgcgatta gtgcttcatt aggtcgcaag ctggatatgc tgaatgctgc
     1381 tgataaagtt ggagatttgc ggtctccccc gggaaaccga tttgagaatc tcaaccctcc
     1441 attgacaggg tattcatcca tacgggtgaa tgagaagtac cgcttgatat ttaagtgggg
     1501 tgaaaacgga gtcgaggatt tgtatctcga tccacatgat taccgataga taacaggtcg
     1561 tggctgttat cttataaaac aagtaggtga ggcgttccct cacctacatt aaaatgtcat
     1621 tacaaataat gattaataaa aaaaatgacg tgactatatt taagataatt actcatcgct
     1681 acagctggta atagacgaaa tacttatctt cctcatctaa cagagagaag taaaaaattt
     1741 aggagtaaag catgtcccaa gcaactagaa aaccatcaac tgtaggggat attattcttt
     1801 atgaatatct cgaaccaagt ggtttaaaaa tcaatgactt ggctgaaatg ttgaatgtgc
     1861 atagaaactc tgccagtgct ttagttaaca ataatagaaa gctgtctacc gatatggctt
     1921 ttaggttggc taaagccttc gataccactg caaagttctg gctaaatata cagcagtctg
     1981 ttgatttatg ggaggttact agtagccctc gattgcaaga agaaatagac agagtgattc
     2041 ccgctcatga aatcatagaa aggaaacaca ttgttgatag ttctgagaag aaacatgcta
     2101 ttgcatgatt ttttttaaaa aaatataagt tatccctcaa aaagaaggct tctataacta
     2161 gaagccttct ttttgaggga taaaaacgct catgttaact ttcttcttac gatcttcttg
     2221 agatccgtgc tctgtacgcg taatctcttg ctctgtaaac gaaaaaaccg cctggggagg
     2281 cggtttttcg aaggttaagt cagttgggga actgcttaac ctggtaactg gcttcagcag
     2341 agcgcagata ccaaatactg tccttccagt gtagccgtag ttaggccacc acttcaagaa
     2401 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2461 tggcgttttg tcgtgtcttt ccgggttgga ctcaagtcga tagttaccgg aacaggcgca
     2521 gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc
     2581 ggaaccgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg
     2641 taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc atcaggggga aacgcctggt
     2701 atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt ctgtgatgct
     2761 cgtcaggggg gcggagccta tggaaaaccg gctctgccgt ggccttatcg cttccctgtt
     2821 aagtttcttc ctggcatctt ccaggaaatt tccgccccgt tcgtaagccc gcaccgctcg
     2881 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaat atatcctgta
     2941 tcgcatattc tgctgacgcg ccgttgccgc cttttttctc ctgccacatg aagcacttca
     3001 ctgatttccg tatccgtgcc aacatattaa gccagtatac actccgctag cgctacgtga
     3061 ctggttcagg gctgcgcccc gaaacccgcc aaaagccact gacgcgcctg cggcttgtcc
     3121 aaccccgcgc cgaccgggaa aggctttccc gcccgtcccg gcgttatcag gaggccggtt
     3181 tgtcagagaa acggaacaag atgctcacca tgtgggtgac ggagggcgag caccggcggc
     3241 tgcttgagcg ctgcgacggc aggccgctgg cggcgtggat gcggcagacc tgcctggacg
     3301 aaaagcctgc gcgcaccgga aagctcccct cactctcgcc ggcgctgctg cgccagcttg
     3361 ccggtatggg caataacctg aaccagatag cccgccgtgt taacgccggc ggcggcaccg
     3421 gccatgacag ggtacagatt gtggcggcgc tgatggccat cgatgccggg cttgagcggc
     3481 tgcggcacgc cgttcttgag aaaggaacgg acgatgatcg ttaaatttca tccacgcggt
     3541 cgcggcggtg gcgccgggcc ggtggactat ctgctgggta aagaccggca gcgtgaaggc
     3601 gccagcgtcc tgcaggggaa gccggaagaa gtccgggagc tgattgacgc gtcgccttac
     3661 gtgaagaaat acacctccgg cgtactgtcg tttgcggagg ccgatctgcc tcccggccag
     3721 cgggaaaaac tgatggccag cttcgagcgg gttctgatgc ccggactcga taaagatcag
     3781 tacagcattc tgtgggtgga gcacgcggac aaggggcgtc tggagctgaa ctttctgatc
     3841 cccaataccg aactgctgac cggcaggcgg ctccagccgt actacgaccg cgccgaccgg
     3901 ccgcgcatcg atgcctggca gaccatcgtg aacggcagac tggggctgca tgacccgaac
     3961 gcgccggaaa accgccgggc gctggtcacg ccgtccggac tgccgaaaac gaagcaggag
     4021 gccactgaag ccatcacgcg cggactgctg acccttgctt cgtccgggga gctgaaaaac
     4081 cgtcaggacg tcactgaggc gctggaacgg tcaggttttg aggtggtgcg caccacgaaa
     4141 agcagtatca gcattgccga cccggacggg gggcgaaaca tccgactgaa gggagccatc
     4201 tatgagcagt cttttaacgc tggcgaagga cttagagcag caatcgaaag cgcagcagca
     4261 gagtaccggc gagatgctga aagccgcatt cagcgagcac gagcagtctg tcagagcgga
     4321 actgagcgca agcgcgagga gaatcagcga cgccatcctc gcacacgagc agagtatgag
     4381 cgaggcgatg gagaaaaacc ggcggagcgt actgcggact gccgggcgga catggttcac
     4441 catcctgatg gtgtcagcgc tgctgatcgg cacgagcggg agcatactgt ggtggcaggg
     4501 gcagcagata accgacaatt acacgcatct tcgtcagcag gaagataccc tggcgaaaat
     4561 gactgcccgg acgtggggag tgcgctatca ggagagcagc gacggccgga ggtttcttat
     4621 cctgccgccc gggatgcaaa ccgaagccat cccctacgac gggacgacat ggatccgcct
     4681 gaaacaggag tagcggaaaa tgacagagct ggaaaagcag ttgctgagcg cattagagca
     4741 gctacagcag gactactcgc aaaggctgga cgaatgggag agc
//
