LOCUS       NZ_CP095154             4350 bp    DNA     circular CON 03-JUN-2024
DEFINITION  Klebsiella pneumoniae strain Iso00073 plasmid pIso00073_04, complete
            sequence.
ACCESSION   NZ_CP095154
VERSION     NZ_CP095154.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17766548
            Assembly: GCF_022964875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Pankok,F., Taudien,S., Dekker,D., Thye,T., Oppong,K., Akenten,C.W.,
            Lamshoeft,M., Jaeger,A., Kaase,M., Scheithauer,S., Tanida,K.,
            Frickmann,H., May,J. and Loderstaedt,U.
  TITLE     Microepidemiology of plasmids in Escherichia coli and Klebsiella
            pneumoniae with acquired extended spectrum beta-lactamase genes
            isolated from chronic wounds in Ghana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Pankok,F. and Taudien,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2022) Insitute of hospital hygiene and infection
            control, University Medical Center Goettingen (UMG), Humboldtallee
            34A, Goettingen, Lower Saxony 37073, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 11-FEB-2022
            Assembly Method                   :: Unicycler v. v0.4.8
            Assembly Name                     :: BNI_Kpneum_Iso00073_hybrid
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022964875.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 03:40:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,710
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,426
            CDSs (with protein)               :: 5,426
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 62 of 160
            Pseudo Genes (incomplete)         :: 92 of 160
            Pseudo Genes (internal stop)      :: 30 of 160
            Pseudo Genes (multiple problems)  :: 20 of 160
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095154.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Iso00073"
                     /isolation_source="Chronic wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pIso00073_04"
                     /geo_loc_name="Ghana: Asante Akyem District"
                     /collection_date="2016"
     gene            complement(join(4195..4350,1..36))
                     /locus_tag="MU411_RS28460"
     CDS             complement(join(4195..4350,1..36))
                     /locus_tag="MU411_RS28460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1380..1949
                     /locus_tag="MU411_RS28465"
     CDS             1380..1949
                     /locus_tag="MU411_RS28465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_087828079.1"
                     /translation="MAKLPLPLPIASPPTVNNSGSERAKKSGRRTLAAKRKTLLEELWG
                     DELEQLKVWNRHEHNGYTTVPRTLTYISRILDYLGGTGLPLSQTYLALWCRVFDEGFVE
                     IRDKDGFAYEAGFSGQRAVTTWTGRMRKLRDLGFITTKPGTSGEFQYVILLNPLTVIKE
                     LYEGKEKDERYNALVGRMQEVGAKWE"
                     /name="hypothetical protein"
     gene            1953..2498
                     /locus_tag="MU411_RS28470"
     CDS             1953..2498
                     /locus_tag="MU411_RS28470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010450637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009310050.1"
                     /translation="MASYFPTLDISGEEFDFTHLEPFIMHVNSDMAKKVLKVHVRFTNH
                     CFTKGYEAFYHPAGEQIIYDHSGNGRTFCPIRYRLSLALPEIINRLCDPAEKVYQTSAK
                     RNWAYSITIDDPEGPYHLFFEIRRASRDEKHLQDLNMVIESAYPEDRGYGPPSLLGRIG
                     FVGLCGRVFCGKPLATKR"
                     /name="hypothetical protein"
     gene            complement(3794..4195)
                     /locus_tag="MU411_RS28475"
     CDS             complement(3794..4195)
                     /locus_tag="MU411_RS28475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_087840457.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGSFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTEFPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          158..561
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.58"
                     /coverage="99.0"
                     /evalue="2.13e-170"
ORIGIN
        1 tgccatattc agcgctgttt tctgttgttt cgtcataaga ggcctcacag cgtcagtgct
       61 cagtgtaggg atgttctctt ttccgcttcc tcgctcactc agtcgctacg ctcctgccgt
      121 tcggctgcgg cgagcggcag gagcatgcgt acaaaagctc gagaaaaccg agcaatcgcg
      181 tgatggggaa aaggaaaggc cgcggcgttg ccgtttttcc ataggctccg cccccctgac
      241 aagcatcaca gaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga
      301 taccaggcgt ttccccctga tggctccctc gtgcgctctc ctgttcctgc ctttcggttt
      361 gccggtgtca ttccgctgtt atggccgcgt ttatctcatt ccacgccaga cactcagttc
      421 cgggaaggca gttcgctcca agctggactg tctgcacgaa ccccccgttc agtccgaccg
      481 ctgcgcctta accggtaact atcatcttga gtccaacccg gaaagacacg acaaagcgcc
      541 actggcagca gccattggta agagtcaatg tgcgagagat tttagagagt acttgaagtg
      601 atggccggac tacagctaca ctgaaagaac agttttggtg actcgtctcg cacaagacag
      661 ttacctggtt aagcagttcc ccaactaact taaccttcga aaaaaccacc tccccaggtg
      721 gttttttcgt tttcagagca agagattacg acgatacaaa tgggatctca agaagatcct
      781 taactaattg cagctcgctt cactatcaca ttcaactccc gtcaccgtcg attaaaacca
      841 tatacaacta cggtcaggtg attccgctca ctggagcgaa attacccgtt aagctcaaaa
      901 cgcttaacgt aggataattc ccgtttccaa gctaacccca tcaacatcca cacatagctc
      961 tcagacgcgc tcaggcaaga gccaagtcat ttcctgcccc aaaccacacc ataaccataa
     1021 aaaccaccga aaatcgaaca cagggcacta tgagcaggca tatcgatttg cattaaatgg
     1081 ctgccgtaaa attattacct tgcccccttg catccccatt cagcaccgcg agcacattca
     1141 gcacattgag cacaattaag cacagcgagc acagaaacct gtcataactc gttgaatatc
     1201 agtcaccata tgctgtattt attacaactc acagattgag ttgtaataaa tacatactca
     1261 caagatacct atacgttgaa aggcaacatc acaaccacac gcaaaacaca acataactca
     1321 ttgttttaaa agaaaaaaca cttaaaataa ccgtatgtta aatgattttg gagaatataa
     1381 tggcgaagtt accactgcca ctaccgatag cgtccccccc aacagtaaac aatagtggct
     1441 cagaaagagc gaagaagtca ggaagaagaa ctcttgcagc aaaaaggaaa accttgttag
     1501 aagaactatg gggagacgaa cttgaacaac tcaaggtatg gaatcgccat gagcataacg
     1561 ggtatacaac tgtgccaaga acgctgacat atatcagtcg aattctcgat tatctcggag
     1621 gaacgggact gccattgtcc caaacatatc tggctctttg gtgcagggta tttgatgagg
     1681 gctttgtgga aataagagat aaagatggat ttgcttatga agctggtttt agtggacagc
     1741 gagcagtaac cacatggaca gggaggatga gaaaactaag agatttaggt ttcataacta
     1801 caaaacctgg cacatcaggt gagtttcaat atgtgatttt gcttaaccca ctgactgtca
     1861 ttaaggagtt atacgaaggt aaggaaaaag atgaaagata caatgcatta gtgggccgta
     1921 tgcaggaggt tggtgctaaa tgggagtaat tcatggctag ctatttcccg acgttagaca
     1981 tatctggaga agagttcgat ttcactcacc ttgagccatt cattatgcac gtaaatagcg
     2041 atatggctaa aaaggtatta aaagtacacg ttaggttcac aaatcattgt tttacaaaag
     2101 gttatgaagc tttttatcac ccagcaggag aacagataat ttacgaccat agcgggaatg
     2161 ggagaacgtt ttgcccaatt agatatagat tatctttggc attacctgag attatcaacc
     2221 gtctatgcga tccagcagaa aaggtgtatc aaacatcagc aaagagaaac tgggcctact
     2281 cgattacgat tgatgatcca gaggggccat atcacttatt ttttgaaata agacgcgcct
     2341 cgcgtgatga gaaacaccta caagatttga acatggttat cgagagtgcc tatcctgaag
     2401 atcgagggta cgggcctccg tccttactag ggcgaattgg ctttgtaggg ttatgcggta
     2461 gggtattttg tgggaagcct ctggcgacta aaagatgaaa aaaaagcacc tgaaaggtgc
     2521 cttttttctg tttctttggt aatcgttccg gacaccacta gcttttgctt aggttcgcct
     2581 tgcgcgaaca caggaacaag atttgtcctg ctgggtagcg ctcaatctca tccggtcagt
     2641 cgctacgatg taaacaatat aacaaaacca ataacgcaat tcagcaccac cggttcaatg
     2701 caatgctttg attcttatgg agtaccatga gtacagaaac cataagtaac catctgaatt
     2761 taaatggcaa gatgcatttt ataaaacaac tcaacttatg agttgtttta taaaatgcat
     2821 ctattcttat gattcaccat gatttcacta caatagaaaa aaacatagat agttgttttc
     2881 attaaaaaaa taaatcccct cctgttttca tcaccttgat gcattggcaa tcgtcgcacg
     2941 gtagctttca ctgataccct cagagggagc tgccgaacag gtcgccggga ggcggcccgt
     3001 ttcgtccctt agcaggaaaa ccttcaggcc ccttcggggc ttttttgcat ctgtcattta
     3061 acataaaggc atgattgtac gcaccgctaa aacggcctca gggcgtttcc gacgggaaac
     3121 cggtaaaaca ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta
     3181 cggcatgcat gagtatgcac gcaagtgcat agcgcgaaaa ttcacgccat tcagccaggg
     3241 taagtcctgt ttccggcgtt cttcctatcc gaaaacggtg aacggggacg caatttattg
     3301 cgtggccgtg aagcgttgcc ggcgcagccg gaaaaccggt tttcagcacg atcaggaatc
     3361 gggttcgtcg acggccggca atcacgttat ggcacgtcgc cggacgactc accacacgat
     3421 tcaccggcga ttcgactcgt gacttgactc tagagtcgtc aaggtcaaaa aaacaccaaa
     3481 gtgacgattc gactctagag tcaagtcgtc ataagttact gatagtctta agtgagtcgt
     3541 cgatgactct atgacgagtc gtcggcaagt cgtacacgaa tcgccgacga cttggattca
     3601 ccttacagaa agtaatactg cgtaatttaa taacccacaa ctaactgata ttaatgagtt
     3661 atcttcaaaa ttttaatttt gcgtggggtg tgggtatttt tagtggtgag gagcgttcag
     3721 agatctgacg atttttggga aggttcagga actccccggg atggccggag aggaaaactg
     3781 aagcatgacg gggtcaggat tttttccaga atttccacca tggcgccggg aattcagtta
     3841 attttgcctg gtgattttca agaaggagca tggcctgttt caggctgtca atgtgcgaat
     3901 ccttggacaa aagtaaggtg ttcaggtgtt caatctcctg tttcagatga cgaatcacct
     3961 gttcactctg gtcagcagag gctgtatcac gctgttcaga gacctgtcca gaatggtgtt
     4021 cacccttcgg ttgctcaaag gaaccaaaga cacgtaaaag ctctgaagta tctaccattt
     4081 ttgcattacg ttcaccgcta caggtggaca ggtcacctgc tttaattaac ctgtgcagtg
     4141 ttgttctact cttacccgtc agtcgggcag cctcactaat agacactctg gccatcagtt
     4201 accatcctgt aatgcatcca gtctcgagga cagtgtacgg aaaagatgct ccgcatcatc
     4261 gtggagtttc tcacaaaggt cggcctcgac atcgagatca agctcgttaa gcttctccag
     4321 caggagcaga gactgattct ggatgaattt
//
