LOCUS       NZ_CP095149             4642 bp    DNA     circular CON 03-JUN-2024
DEFINITION  Klebsiella pneumoniae strain Iso00100 plasmid pIso00100_04, complete
            sequence.
ACCESSION   NZ_CP095149
VERSION     NZ_CP095149.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17766553
            Assembly: GCF_022964835.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Pankok,F., Taudien,S., Dekker,D., Thye,T., Oppong,K., Akenten,C.W.,
            Lamshoeft,M., Jaeger,A., Kaase,M., Scheithauer,S., Tanida,K.,
            Frickmann,H., May,J. and Loderstaedt,U.
  TITLE     Microepidemiology of plasmids in Escherichia coli and Klebsiella
            pneumoniae with acquired extended spectrum beta-lactamase genes
            isolated from chronic wounds in Ghana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Pankok,F. and Taudien,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2022) Insitute of hospital hygiene and infection
            control, University Medical Center Goettingen (UMG), Humboldtallee
            34A, Goettingen, Lower Saxony 37073, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 16-FEB-2022
            Assembly Method                   :: Unicycler v. v0.4.8
            Assembly Name                     :: BNI_Kpneum_Iso00100_hybrid
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022964835.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 03:33:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,536
            CDSs (total)                      :: 5,415
            Genes (coding)                    :: 5,258
            CDSs (with protein)               :: 5,258
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 60 of 157
            Pseudo Genes (incomplete)         :: 103 of 157
            Pseudo Genes (internal stop)      :: 12 of 157
            Pseudo Genes (multiple problems)  :: 17 of 157
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095149.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Iso00100"
                     /isolation_source="Chronic wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pIso00100_04"
                     /geo_loc_name="Ghana: Asante Akyem District"
                     /collection_date="2016"
     gene            complement(join(4635..4642,1..1675))
                     /locus_tag="MU412_RS27575"
     CDS             complement(join(4635..4642,1..1675))
                     /locus_tag="MU412_RS27575"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage dCTP deaminase"
                     /protein_id="WP_223862165.1"
                     /translation="MSLARKLREKRSNPIHSANEDSSGGDSINQITSRQSMDLVIGFCG
                     AVGSGIKSIVDVTEEELDDLGYDVYRIRVSELMQKLFSEWRDDIVTPFDRYHKLQDFGD
                     ALRNKYYPHILSEAVIHEIKIEKGIDTSSKKNKKAFLVDQLKHQDEIELLRMVYQHNFY
                     LLGVIRSEVERKRNLRDEGLSKSEVDIVIHHDRKSESDYGQQTAKAILDSDYFVKNNQG
                     QRAHLKAKVKRFIGLIHGLNGLTPTTYEKGMYAAHSASLQSACLSRQVGAAILDVNGNL
                     ISVGRNDVPKFNGGLYSADDGVNDHRCVYKGGKCYNEVRKLKIKSKISQILMSESACEL
                     GVKLTEDQAEQLADVIYKKTPVSSIIEYSRSIHAEMDAITSLARLGNGGFSDKVLYTTT
                     FPCHNCARHIVAVGITKVVYIEPYEKSLALELHDDAITEVNEHGKVIFESFEGVSPRRY
                     QKFFFSTDERKDSFGNAEKYSTKYKNHIDIQFIDSYLDFENRVVDIFRTNTNIDSGNHV
                     GRTDSTINLSSQPVPPVPPVPPVPPVPPVPPVPPVPPVPPDSK"
                     /name="anti-phage dCTP deaminase"
     gene            complement(1688..1870)
                     /locus_tag="MU412_RS27580"
     CDS             complement(1688..1870)
                     /locus_tag="MU412_RS27580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080882243.1"
                     /translation="MGKELSLNKIYDKNSRYINDSRGHELGKEKKVFDFEVEMIKKRRE
                     DAIRKIIEDSKSLKW"
                     /name="hypothetical protein"
     gene            2258..2467
                     /locus_tag="MU412_RS27585"
     CDS             2258..2467
                     /locus_tag="MU412_RS27585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_085808090.1"
                     /translation="MKSIKTFVAVVALSMMSFGAFAQSVSATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     gene            complement(3310..3711)
                     /locus_tag="MU412_RS27590"
     CDS             complement(3310..3711)
                     /locus_tag="MU412_RS27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3711..3902)
                     /locus_tag="MU412_RS27595"
     CDS             complement(3711..3902)
                     /locus_tag="MU412_RS27595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4318..4431
                     /locus_tag="MU412_RS27720"
     CDS             4318..4431
                     /locus_tag="MU412_RS27720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_080882241.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     Typing          4024..4431
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.355"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 agtctggtgg tactggtggt actggcggta cgggcggtac gggcggtacg ggcggtacgg
       61 gtggtacggg cggcacgggc ggcacgggct gggaagataa attaatagtc gaatcagttc
      121 tcccgacatg attgccagaa tcaatatttg tgttagttct aaaaatatca acgactcgat
      181 tttcgaaatc taaatagcta tcaataaact gtatatcaat gtgattttta tatttagtgg
      241 agtacttttc tgcgttaccg aaactatctt tcctttcatc tgtagaaaag aagaattttt
      301 gatacctacg aggtgataca ccttcaaagg actcaaatat aaccttccca tgctcattca
      361 cctcggttat agcatcatca tgcaattcta aagcgaggct tttttcatat ggctctatat
      421 aaacaacttt tgttattcca acagcaacaa tatgtctcgc gcagttatga caaggaaatg
      481 tggttgtata taaaacttta tcagaaaaac ctccattacc tagtctagct aatgatgtaa
      541 tagcatccat ctctgcatga attgagcgag agtactctat aatagaagat actggtgttt
      601 ttttatatat tacatcagcc agttgctctg cttgatcttc tgttaattta acaccaagtt
      661 cacaagcgct ttccgacata agtatttgtg aaattttgct tttaatcttt agctttctaa
      721 cttcattata acatttacca cctttgtata cacaacgatg atcattgaca ccatcatccg
      781 cactataaag cccaccattg aatttaggta catcattacg cccaacggaa atcagattcc
      841 cattaacatc caatatagca gcaccaactt gacgagaaag gcatgcagat tgcagagatg
      901 ctgaatgagc tgcatacata cctttttcat acgtggttgg agttaaacca tttagcccat
      961 gtatcaagcc aataaaacgc ttaaccttag ccttaaggtg cgccctttgt ccttgattat
     1021 tcttaacaaa ataatcagag tcaagtatag ctttcgcagt ctgctgacca taatcgctct
     1081 ctgattttct atcgtgatga ataactatat cgacttctga cttactcagc ccttcatctc
     1141 taagattacg ctttctttct acttcacttc taattacacc tagtaaataa aaattatgtt
     1201 ggtagaccat tcttaaaagt tcaatttcat cctgatgctt caactgatcg accaaaaaag
     1261 cctttttatt ttttttactt gaggtgtcaa tgcctttttc tatttttatt tcatggatta
     1321 cagcttcaga tagtatatgt gggtaatatt tatttcttaa cgcatcacca aaatcttgca
     1381 gcttatgata tctatcaaac ggagtaacaa tatcatcacg ccattctgaa aaaagttttt
     1441 gcataagctc gctaactctt atgcgataaa catcatatcc tagatcatct agttcttctt
     1501 cagtcacatc aacaattgac tttattcctg agccaacagc accgcaaaat cctataacaa
     1561 gatccataga ctgtctggag gttatttggt ttatgctatc ccctccagag gagtcctcgt
     1621 tagcactatg aatcggatta cttctttttt ctcttaactt tctagccagg ctcataaaag
     1681 acctcactta ccattttaat gattttgaat cctcaattat cttccttata gcgtcctctc
     1741 ttcttttttt aatcatttca acttcaaaat cgaaaacttt cttttccttt cccagctcat
     1801 gccctctaga atcattaata tatctagaat tcttatcata aattttattt agagatagct
     1861 ctttacccat cacaccctcc tgcaagacgc atggatttta ctgttatctt tataacaggc
     1921 aatagccaag aaacgtaatc gatagagatt tttttttctt atcctaaaca ttaggaaatt
     1981 cggcaagaga aaagcaagag agatcacgcc aaactaacca ttatttttca atttgttaaa
     2041 ataaaatcat caatgttaac atttctatcc accacctcat ttggtaccta gaatccagag
     2101 ataaacctct ggtcttgatg cgctcaattc ttgttctttt tttttgaatg atatccgcaa
     2161 attgtttcga ttttaatcag tccatggtcg tcctatcatt tatcacatca gggcaacaac
     2221 gccccataca gaacaaaatt acttaaggaa tacgaccatg aaatccatca aaactttcgt
     2281 tgcagttgtc gcactatcca tgatgtcttt cggtgccttt gctcagagcg tcagcgctac
     2341 cgcctccacc ctggatcgtg ctgaagcaaa aattgccgca caggccgctg aacaaggtgc
     2401 gtcatacaaa atcaccagcg ctcagttcaa taaccgtgtc cacatgacgg ctgaactgac
     2461 caaataagat ggtcgcagag ggagctgccg aacaggtcgc cgggaggcgg cccgtttcgt
     2521 cccttagcag gaaaaccttc aggccccttc gggggctttt ttgcatctgt catttaacat
     2581 aaaggtatga ttgtacgcac cgctaaaacg gtctcagggc gtttctgacg ggaaaccggt
     2641 aaaacaggca acaaaccacc cgaaaacccg ccagaaacgc gcaggctctg tttttacggc
     2701 atgcatgagt atgcacgcaa gtgcatagcg cgaaaattca cgccattcag ccagagtaag
     2761 tcctgtttcc ggcgttcttt ccatccgaaa acggtgaacg gggacgcaat ttattgcgtg
     2821 gccgtgaagc gttgccggcg cagccggaaa accggttttc agcacgttca ggaatcgggt
     2881 tcgtcgacgg ccggcaatca cgttatggca agtcgccgga cgactcacca cacgattcac
     2941 cggcgactct tcactagagt cgtacccaga gtcgccgcgt ctaaaaaaca accaaacggg
     3001 gagtcgtgtc tagagtcggg tcgttattaa cttattgttt ttatagtcct gagtcgttac
     3061 cgactctatg acgactcgcc ggggactcta tgacgagtcg tcatagagtc ggatctatgt
     3121 aaataaattt taaaaaccga aatttcagga aagtaagcta ctgatattaa atacaaatct
     3181 gcaaaatttt aattttgcgt agtgtgtggg tatttttagt cgtgaggagc gttcagagat
     3241 ctggcgattt ttgggaaggt tcaggaactc cccgggatgg ccggagagga aaactgaagc
     3301 atgacggggt caggattttt tccagaattt ccaccatggc gccggggatt cagttaattt
     3361 tgcctggtga ttttcaagaa ggagcatggc ctgtttcagg ctgtcaatgt gcgaatcctt
     3421 ggacaaaagt aaggtgttca ggtgttcaat ctcctgtttc agatgacgaa tcacctgttc
     3481 actctggtca gcagaggctg tatcacgttg ttcagagacc tgtccagaat ggtgttcacc
     3541 cttcggttgc tcaaagggac caaagacacg taaaagctct gaagtatcta ccatttttgc
     3601 attgcgttca ccgctacagg tggacaggtc acctgcttta atcaacctgt gcagtgttgt
     3661 tctactctta cccgtcagtc gggcagcctc actaatagac actctggcca tcagttacca
     3721 tcctgtaatt catccagtct cgaggacagt gtacggaaaa gatgctccgc atcatcgtgg
     3781 agtttctcac aaaggtcggc ctcaacatcg agatcaagct cgttaagctt ctccagcagg
     3841 agcagagact gattctggat gaatttagcc atgttcagcg ctgttttctg ttgtttcgtc
     3901 ataagaagcc tcagagcgtc agtgctcagt gtaagaacag tctcttttcc gcttcctcgc
     3961 tcactcagtc gctacgctcc tgccgttcgg ctgcggcgag cggcaggagc atgcgtgtaa
     4021 aagctcgaga aaaccgagcg gtctcgtgat ggggaaaagg aaaggccgca cagcggccgt
     4081 ttttccatag gctccgcccc cctgacgagc atcacggaat ctgacgctca aatcagtggc
     4141 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctgatggc tccctcgtgc
     4201 gctctcctgt tcctgccatt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt
     4261 ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg
     4321 cacgaacccc ccgttcagtc cgactgctgc gtcttatccg gtaactatca tcttgagtcc
     4381 aacccggtaa gacatgcaaa agcaccactg gcagcagcca ccggtaactg atttagagga
     4441 gttagtcttg aagttatgcg ccagttaagg ctaaactgaa agaacaagtt ttggtgactg
     4501 cgctcctcca agccagttac cttggttcat agagccgcaa actctagaac cttaaaaaaa
     4561 ccgccccaca aaggcggttt ttttctttct cgtctcaagg ttaagctctg tataacatca
     4621 gagtcaacag atcttcattt cg
//
