LOCUS       NZ_CP095148             8282 bp    DNA     circular CON 03-JUN-2024
DEFINITION  Klebsiella pneumoniae strain Iso00100 plasmid pIso00100_03, complete
            sequence.
ACCESSION   NZ_CP095148
VERSION     NZ_CP095148.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17766553
            Assembly: GCF_022964835.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Pankok,F., Taudien,S., Dekker,D., Thye,T., Oppong,K., Akenten,C.W.,
            Lamshoeft,M., Jaeger,A., Kaase,M., Scheithauer,S., Tanida,K.,
            Frickmann,H., May,J. and Loderstaedt,U.
  TITLE     Microepidemiology of plasmids in Escherichia coli and Klebsiella
            pneumoniae with acquired extended spectrum beta-lactamase genes
            isolated from chronic wounds in Ghana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8282)
  AUTHORS   Pankok,F. and Taudien,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2022) Insitute of hospital hygiene and infection
            control, University Medical Center Goettingen (UMG), Humboldtallee
            34A, Goettingen, Lower Saxony 37073, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 16-FEB-2022
            Assembly Method                   :: Unicycler v. v0.4.8
            Assembly Name                     :: BNI_Kpneum_Iso00100_hybrid
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022964835.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 03:33:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,536
            CDSs (total)                      :: 5,415
            Genes (coding)                    :: 5,258
            CDSs (with protein)               :: 5,258
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 60 of 157
            Pseudo Genes (incomplete)         :: 103 of 157
            Pseudo Genes (internal stop)      :: 12 of 157
            Pseudo Genes (multiple problems)  :: 17 of 157
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095148.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Iso00100"
                     /isolation_source="Chronic wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pIso00100_03"
                     /geo_loc_name="Ghana: Asante Akyem District"
                     /collection_date="2016"
     gene            complement(638..970)
                     /locus_tag="MU412_RS27520"
     CDS             complement(638..970)
                     /locus_tag="MU412_RS27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321410.1"
                     /translation="MEKQYTNELTAEILAGMDQSPFTPEQLAAMSDEARALIEEQEAFC
                     HAHPVTTIYRLAVAGCLTRRGGTGDEFNPNPEEGHKIRLENGLWVSVLTEGCTVTYPDG
                     TQARIL"
                     /name="hypothetical protein"
     gene            1124..1375
                     /locus_tag="MU412_RS27525"
     CDS             1124..1375
                     /locus_tag="MU412_RS27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321409.1"
                     /translation="MALVQKKTLNELATNASDDFFGKRFSAGDEVPHSGIYKCTKCNRE
                     VTVNAHSRDNTFPPHYPESPCKSPTWKPYVIADTKAEW"
                     /name="hypothetical protein"
     gene            1388..1642
                     /locus_tag="MU412_RS27530"
     CDS             1388..1642
                     /locus_tag="MU412_RS27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321408.1"
                     /translation="MNFKQLIPAQDWYFRHFTDQSDYPVIVYQLAAWALTEEGSITGLV
                     AIYDPKEGKAKLVTPPPVKGNYLHRDQLTDEELAASRKP"
                     /name="hypothetical protein"
     gene            complement(1673..2191)
                     /locus_tag="MU412_RS27535"
     CDS             complement(1673..2191)
                     /locus_tag="MU412_RS27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044704828.1"
                     /translation="MQKVTDLYPPEIARHKLQNHFAGNTVMLELIQKLNKTSLCTFAAL
                     CDGNVVTTSGYNITADLCVNRASAVAHSLKQKYLPVTARTISTKADVGGAVKQAAFCID
                     EDDLARLKSEPEKMMKECERNLNSQKRTNAQKEMSRLYKEFGEDGILALLRNVARSNGT
                     PPPGAHPAS"
                     /name="hypothetical protein"
     gene            2965..3228
                     /locus_tag="MU412_RS27540"
     CDS             2965..3228
                     /locus_tag="MU412_RS27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            3218..3517
                     /locus_tag="MU412_RS27545"
     CDS             3218..3517
                     /locus_tag="MU412_RS27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010848978.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_009365129.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYEVDDPAQKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3579..3956)
                     /locus_tag="MU412_RS27550"
     CDS             complement(3579..3956)
                     /locus_tag="MU412_RS27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321415.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQGETHERDGLRLPDETEKTASEALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETVETPAETPRRWWHWGKR"
                     /name="hypothetical protein"
     gene            4026..4205
                     /locus_tag="MU412_RS27555"
     CDS             4026..4205
                     /locus_tag="MU412_RS27555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032934837.1"
                     /translation="MTKQEKTALNMAKFIRDQSLLLLEKLNELDMDDPADMCERLHEDA
                     EKLWLVMRGKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4233..4763)
                     /locus_tag="MU412_RS27560"
     CDS             complement(4233..4763)
                     /locus_tag="MU412_RS27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044704832.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMAVMAWQTGAYVFRFE
                     DQAQHLAMMKFIGMYHERLNSDDKATYYFTGKNPAWERDKPSGMKLCDYLYRKVEQFAI
                     ENGVQVLDAGKYTPEQIGAMIDVVSERHGESAVNEKLKAELMREYNSRYFNEPVPVLPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4770..5501)
                     /locus_tag="MU412_RS27565"
     CDS             complement(4770..5501)
                     /locus_tag="MU412_RS27565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="WP_008321412.1"
                     /translation="MALEQYNVSHAKRQARNAEKTRLVLRWLRDELCSTAETVARLLGI
                     GAVQPVYRFLDSLVAKGLLARAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLDMEARGATGWRTLHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            complement(5501..7462)
                     /locus_tag="MU412_RS27570"
     CDS             complement(5501..7462)
                     /locus_tag="MU412_RS27570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_044704830.1"
                     /translation="MLNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNDRAAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLVLLGGLVVAVFIESWGETVLRPGYYANADYYLLQPANLFGIAWCAGGLV
                     VGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARQQQVVFLGKDEDGQPVRVPREAWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLAETGDVADHYRISEQKAAKLIAEQFPHGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRAWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QAEAVRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     Typing          complement(175..354)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.659"
                     /coverage="90.0"
                     /evalue="1.1199999999999999e-60"
     Typing          complement(4774..5501)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.239"
                     /coverage="100.0"
                     /evalue="1.12e-141"
ORIGIN
        1 ctccgccctc ctgccagagt tcacataaac agacgctttt taggtgcatc tgtgggagcc
       61 gtgaggctca accatgaatc tggcagtacg ggcgaaaccc gacaggactt aaagatcccc
      121 acagtttccg gtgggtcgct ccctcttgcg ctctcctgtt ccgaccctgc cgtttaccgg
      181 atacctgttc cgcctttctc ccttgcggga agtgtggcgc tttctcatag ctcacacact
      241 ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg ctgtaagcaa gaactccccg
      301 ttcagcccga ctgctgcgcc ttatccggta actgttcact tgagtccaac ccggaaaagc
      361 acggtaaaac gccactggca gcagccactg gtaactggag ttcgcaggaa cgtaagagtg
      421 aaaaagtaac tttttcgctt gaagaagcca gtttgaagca gctaaactga ctgtactgtt
      481 ttggtgactg ttcctgcgaa gatcagttac cgcccggttt cggcgagttt gccgctcgac
      541 gaacccgaac taacccgcct tacggcgggt tttttcgttt acagagcaag agattacgcg
      601 cagtggtaaa cgatctcaag tggatctttt taagatccta cagaatccgg gcttgcgtgc
      661 catcaggata cgtcacggta caaccttcag tcagcacgct gacccacaac ccgttctcca
      721 gccggatttt atggccttcc tcagggttcg gattgaactc atcgccggta ccaccgcgcc
      781 gggtcagaca gcccgccacc gccagtcggt aaatggtcgt caccggatgc gcatggcaga
      841 atgcctcctg ctcctcaatc aacgcgcgtg cttcatcgct catcgcagcc agttgttcag
      901 gagtaaaagg tgattgatcc attccggcca gaatctccgc ggtcagttca ttggtgtact
      961 gcttctccat actaatttct tgtactttaa atggttaaaa tcacgccatc atactcataa
     1021 tcctgactta accaaaactc agccgcttga aaaaacgcca caaagaccac atttataaaa
     1081 tgggtaagaa tgcccgaaaa atatcgatgg gaaggaaagc agaatggcct tagtacagaa
     1141 aaaaacgctg aatgaactgg caacaaacgc cagcgatgac ttcttcggta agcgattttc
     1201 agccggagat gaagttccac actcggggat ctacaaatgc acgaaatgta atcgtgaagt
     1261 gacggtaaat gcccactcca gagacaatac atttccgcca cattacccgg aaagcccgtg
     1321 taaatcaccg acgtggaaac cctatgtcat tgcagacaca aaagcggaat ggtgaaggag
     1381 ctaatacatg aactttaagc aactcatccc cgcacaggac tggtattttc gtcattttac
     1441 cgaccagtct gattatccgg tgatcgttta tcagcttgct gcatgggcac tgactgagga
     1501 aggaagcatt accggccttg tggcgatata cgatccgaaa gaaggcaaag caaaactggt
     1561 gacaccaccg ccggtaaaag ggaactatct gcaccgagat cagctgacag atgaagagct
     1621 ggcggcatcc agaaaacctt aaaaataaag cccctgaggg gctttactgg tatcagcttg
     1681 cagggtgtgc gcctggcggc ggtgtaccgt tcgaccgggc aacattgcgt aacagcgcca
     1741 gtatgccgtc ttcaccaaac tccttgtaca accgactcat ttccttctga gcattggttc
     1801 gcttctggct gttcaggtta cgttcgcact ctttcatcat cttttccggc tctgatttca
     1861 gccttgcaag atcgtcttca tcgatacaga aagccgcctg ctttaccgca ccacctacat
     1921 cggccttagt cgaaatcgtt ctggccgtaa ccggcagata tttttgctta agcgaatggg
     1981 ccacggctga cgcacggtta acacacagat cggccgtaat gttatagccg gaagtggtca
     2041 cgacgttgcc gtcacagagc gccgcaaaag tacacaggct ggttttattt agtttctgga
     2101 ttaactccag cataacggta ttaccggcga agtgattctg gagcttatgg cgagcaattt
     2161 caggaggata cagatcggtt actttttgca tttatagcac ctgtttacag aggataaata
     2221 acaggtgacg gtgtccggat gagccttcgc acatcaaaaa actgtgaaaa atgcctggca
     2281 agtcggtacc aagttactac ataatgcagc accacttgac aaacgcccta atcgggcgtt
     2341 tgttgatcag gtaagattca gttttcgcga actgagtcac cggcagctat cagaatcctt
     2401 agtttctcag gccgaagatc tgatagctgc ctcgtttttc actgacaacc tcacgatcat
     2461 cagaatccct tattctaccc tattccataa gcgcacacat agcttttttg ctgatctttc
     2521 gcgcttgatt tacaaggaaa ttacctgtcg ggtagatgaa attagcaaca gcgctatcag
     2581 atgacgctaa tgttaaactc atgtttaaac atacagcgaa aagttgacgc aaaaatcgtc
     2641 agctggatgc ctcacaatcc cgtcatttac cataacatcc gttgcccgca ccccctgaga
     2701 gcctctgagg gcatttctga cgggaaaccg ataaaacaga caacaaacca cccgcaacac
     2761 ccccggaaac gcgccggata aattgtacct gaacgcatgg ctatgcacgt aactgcatgg
     2821 catgggattt gacgtaaaat ggatgccgta agcccttttt taccgacttt ctgcttcatc
     2881 agcctgactg acacgagcat attctttcag gccgcctcct gctaaacttt ttcatattgc
     2941 cgcattaacc cgggaggctc cgcaatgagc aacacgacac actacgaaaa cgccaacttt
     3001 cttcgggaac tggctgaaag cctgccccgc atactgccgg aaggtggccc ggacaaagct
     3061 gcgctgttgc agcgcctggc taacgaagaa ctggcccagg cagagtatga agatcaggtt
     3121 cgcgccaaag tgaccgccgc ccgtgctgac acgcgtccgg gcatgaccac ggaacaactc
     3181 cgccagcgcc tgcatggccg ttatcaggaa ctgcgcgatg ccgtatgacg tggaatggaa
     3241 gcagggagcc atcgaagatg taacgacact gtttgactat atcgcggaaa attcatcgct
     3301 gtgggatgcg cagaacgtta ccgaccgggt tctcgctgct gcggacaaac tggcggactt
     3361 cccgcgcctc tacgaagtgg actcacgtta tggcgaagac gttcgccgga tcagcctgat
     3421 gggacaacat gtcctctatg aagtggacga ccccgcgcag aaagtccggg ttctggccgt
     3481 ggtcggccag cgccagaatc cccataccgt tcgctaataa ccggtgacag gggcgcaatt
     3541 cattgcgtgc ccgtcgccgg ttggacaagc gcagcgcgtc agcgttttcc ccaatgccac
     3601 caccggcgcg gcgtttcggc cggtgtctct acggtctccg gtttgtgttc cagcagtctc
     3661 atggcctgca tttcctgccg caacccggcc acctcctccc tgagtgcttt tatctccgcg
     3721 atcaacgctt cagaggccgt tttttcagtc tcatctggca gtctcaaccc gtcccgctcg
     3781 tgagtctcac cctgtcttaa ctcaccgtac acccggataa gctcgctggt atccacaacc
     3841 cgaccgccat cagcctccgt ccggtatgat acgcggcctt tcgccatatc ccggtacagg
     3901 gaactccggg ctttccccgt cagttccgtc gcctgtctca ccgtcaccca cgccattgtc
     3961 tcaccccgct atttttgaga cagtataccc gactggcgca caccatgccc ggaagggaaa
     4021 aaatcatgac gaagcaggaa aaaaccgccc tcaacatggc aaaattcatc agggatcagt
     4081 cgctgctgtt gctggaaaag ctgaacgagc tggacatgga cgacccggcc gacatgtgcg
     4141 aacgacttca cgaggatgcg gaaaaactct ggctggtaat gcggggtaag ctcggggaag
     4201 aataacagca ccggcatggc cggtgctggc gtttattttt tgccgaacgg ccaccaccgg
     4261 cgggcgggtt tatcctccgg caatacgggc accggctcgt taaaataccg gctgttatac
     4321 tctcgcatca gctccgcttt cagcttctcg ttcaccgcac tctcaccgtg ccgctcactc
     4381 accacgtcaa tcatcgcgcc gatctgctcc ggggtgtact tccctgcgtc cagtacctgc
     4441 acgccgttct cgatggcgaa ctgctcaacc ttgcggtaaa gataatcaca cagcttcatt
     4501 ccggacggtt tatcacgttc ccacgccgga tttttgccgg tgaagtaata cgtggctttg
     4561 tcatcgctgt tcagccgttc gtgatacatg ccgataaact tcatcatcgc cagatgctgg
     4621 gcctgatctt caaaccggaa tacatatgcc ccggtctgcc aggccatgac cgccatgtct
     4681 tccagatgct cgctggttgg cataatgcag cgctcgccgt ctttgtcttc cacatcctca
     4741 ataaaaacca tgtacagcgc cataatccct cactctgttg tcataaattt aaagcacgcg
     4801 aacagcctct cccggtccag ttccgccgtt ttgcgggctt cgccggtctg cgggttcacg
     4861 atttcatcca ccgccagaat cgcccgctgt acccggacaa gcgtcggccg gtcggggcag
     4921 atgtaccaga tttcatcgat gccgttcgcc ttacggttga acagataccc ggacaccact
     4981 tcctgatacc ggcgacggct cttgacggtg cgctccacct caaacgccac cgttttaccg
     5041 tccagctctg ccagcgcatc cggcaccttc atacctttaa gactcaggcg gtgcagggtg
     5101 cgccagccgg tggcccctct ggcttccata tcaagacgca gggactgcac ggccagccgg
     5161 tgcggcagct gcgccgccga cacccgcgag ggctgaaacg gaataacgtc cgtcagcggc
     5221 tcgtcctcgt cgaacgagaa cgccacgccg tgcggggtca gcccccacac gctgacctga
     5281 cgaccgtcaa ccgggtattt cgcccgggcc agcagtcctt tcgccaccag gctgtccaga
     5341 aagcggtaaa ccggctgcac cgccccgatc cccagcagac gggctacggt ttcagccgtc
     5401 gagcagagtt cgtcccgcag ccaccgcagg actagccggg ttttctccgc gttccgcgcc
     5461 tgtcgcttcg cgtggctgac gttgtattgc tccagagcca tcagtacagc cccgcaaaat
     5521 catcgtcacc cgggcgctgc cgcactgcct cagcctggta ctccgccagc gggtccgttt
     5581 tcgccagcac cgggaaggtt ttcagcggaa tttcccggcg atcaacccgc agcgggctgc
     5641 tgaacgccag ccgcgccacg cccagcccgg tcatgaccgc gaacccgtcc ggcaggtgtt
     5701 gcagggtgtt cacgtcgaac agcggccgcg cttccttctg gatgaagctg tccccgctga
     5761 tgtgctccac gttccccgcc tcccggctgg cccggcggcg ctccacgtcc acccggattt
     5821 ccccggtctg tccggccgcc cactgcgcgg actcctggct ggccgcgcga tagaaccagc
     5881 ggattggcgt gttatccagt accgtggttt tcacaaaatc agcgggcaga tcctgcccgc
     5941 actggccgaa gtcgcccagc gactggtgag ccagcagcag gttacagtcc cggctgcgca
     6001 gcgtccccag cgcgttgagt acgtacttcg agagcagata tttgatttca tcgagcatca
     6061 cgctgacgtg cggccacgcg cggaactcgt cccgcgcaat gatgatttgc gcgcagcggg
     6121 caaacagcat tttctgcacc cggatcaccg cctcgtcgtc catcgatccg atgacgtaca
     6181 gacagccgcc gccgttgata atccccgcca catcaatacc ggaatccgtc tgcaggacgc
     6241 tcaggtccgc gacgttctcc agcttggtga tcagcccctt cacgtccttt ttcagcgcct
     6301 ccggcagcgc atacgccgcc gccagcacct gctgaatgtt cgcgccgtgc gggaactgct
     6361 ccgcgataag cttcgccgct ttctgctcgc tgatgcggta gtggtcggcc acatcgccgg
     6421 tttccgccag gttaaacccg gcaaccagca gatttttcag tgcgtactgg tcaaggtcgc
     6481 ggaacaggtt cagctgcgcc ggtttccccg gccgcaggtc cagcagggtg aagtccgggc
     6541 agtgcgcccg aaagacgtgt ggcgcccatg cgtcgccttt cgggtcgaag tacaccaccg
     6601 catcgccaaa atcgcgcacg cagcggatca gcgcgttggt ggccatcacg ctcttgccgc
     6661 tcccgggtag gccgaggatc tgaatattgg tcttgcgcca cgcctcgcgc ggaacccgca
     6721 ccggctggcc gtcctcgtcc ttacccagga acaccacctg ctgctgccgg gcctgctccg
     6781 tggaaaaatc ggccagcgcg ttgaccggca ccggctcccc gaaatgcagg tggcgcacgt
     6841 cagtcatttc gccggtgcgc tggctgctgc gccggctctc ctgattcagt tttgcattga
     6901 ccgcctcgtc agcggccgca aaatgaccgt caagcagcag gaatgccagt agccgcgcca
     6961 gaagtatccc gacgaccaga ccaccggcac accaggctat cccgaacaga ttagcgggtt
     7021 gcagcaggta ataatcggca ttcgcgtagt agcccggacg caacaccgtt tctccccagc
     7081 tctcgataaa cacggcgaca accagccccc ccagcagaac cagaccggca gcgccggtca
     7141 gccagtacgc gtagttccag acatgtcgac gagccctgac catccgcccg ggagaatcca
     7201 gcgaggccaa caccgaaaat ggcgcagccc tgtcattatc gcgcttatag cgatcggcat
     7261 actcccgccg ctccttatgg ctcaggccca caaaacgcat ccgtccggcc cagagttcag
     7321 ccagaccgaa aaacaggccg ataaccagaa aagccccggc cagaccgtaa tactcccggt
     7381 acagccagct gacccacggg taaatctgcc gggccagctc cgggatatgc cactgccagg
     7441 caagcgaatc cgtattcaac atgcaacctc tctcctacag acgacggatt taccggcgtg
     7501 atgcgcagcc catctgcgca agctgacgac atgccccaag gcatggcggc ggcgttcacg
     7561 tccgtggtga aagcgttcag cgctcgccac ggaggccagc ctctttccgc cccatgcgga
     7621 aaaggcgcag ccccggagga acaacgagca cgcccacagc gtgcgagagg aaaaggcggc
     7681 accccagtgc tgtcttttcc ggtgttccgg cccccgtcag agtaagcgac agcgagcggc
     7741 tgaccaccca taaaatcagg agcagctttg ctgcttcgtc aggtgcgtcc agcaccgtgg
     7801 atttttgtgg gtggtcaggc gtgagcctga cgggggccgg gtgaatttct ggcgtgaccg
     7861 gcgtccagcc gggctaagac agaaagaggg gtaaagacgc tgcgtcggtg gcgttcagcc
     7921 agcgtaagca ggggatttac ccctgaagtc agtcccgccc tagcggggat gacttgcatt
     7981 tctccgcaag gagaaaaaga aacagagata ggttaacgta aaatgtgtat cagaaaaccg
     8041 atatacttca cctatcctga tgatttatcg acagggaaca gaaaggcttt aatcaggagc
     8101 aaaatgtcac atcagcgcgt ccggtggccg taggcaaaaa cgggagatat tgcgcctcct
     8161 cgctcactcg gtcgctacgc tccggccgtg aaactgcggc gggcgctgcg gactcacgag
     8221 cagggcaaac aggcatagag agaaaataat acgaagctgc gccggtggcg tttttccata
     8281 gg
//
