LOCUS       NZ_CP095144             3643 bp    DNA     circular CON 03-JUN-2024
DEFINITION  Klebsiella pneumoniae strain Iso00199 plasmid pIso00199_04, complete
            sequence.
ACCESSION   NZ_CP095144
VERSION     NZ_CP095144.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17766581
            Assembly: GCF_022964855.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Pankok,F., Taudien,S., Dekker,D., Thye,T., Oppong,K., Akenten,C.W.,
            Lamshoeft,M., Jaeger,A., Kaase,M., Scheithauer,S., Tanida,K.,
            Frickmann,H., May,J. and Loderstaedt,U.
  TITLE     Microepidemiology of plasmids in Escherichia coli and Klebsiella
            pneumoniae with acquired extended spectrum beta-lactamase genes
            isolated from chronic wounds in Ghana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Pankok,F. and Taudien,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2022) Insitute of hospital hygiene and infection
            control, University Medical Center Goettingen (UMG), Humboldtallee
            34A, Goettingen, Lower Saxony 37073, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 16-FEB-2022
            Assembly Method                   :: Unicycler v. v0.4.8
            Assembly Name                     :: BNI_Kpneum_Iso00199_hybrid
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022964855.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 03:43:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,613
            CDSs (total)                      :: 5,485
            Genes (coding)                    :: 5,358
            CDSs (with protein)               :: 5,358
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 56 of 127
            Pseudo Genes (incomplete)         :: 74 of 127
            Pseudo Genes (internal stop)      :: 15 of 127
            Pseudo Genes (multiple problems)  :: 16 of 127
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095144.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Iso00199"
                     /isolation_source="Chronic wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pIso00199_04"
                     /geo_loc_name="Ghana: Asante Akyem District"
                     /collection_date="2016"
     gene            complement(join(3630..3643,1..328))
                     /locus_tag="MU413_RS27945"
     CDS             complement(join(3630..3643,1..328))
                     /locus_tag="MU413_RS27945"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_049087621.1"
                     /translation="MFTLQKPMHPGEFIREVYLEPLEIQISTFAEKIGVDTSTISRLVN
                     QKADLSYEMAIRLSKAFGRSAESWMKMQIAYGLYRAQDKVNLDEVETIYTPRNLDAAEM
                     QDHEPYINL"
                     /name="HigA family addiction module antitoxin"
     gene            595..870
                     /locus_tag="MU413_RS27950"
     CDS             595..870
                     /locus_tag="MU413_RS27950"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_049087620.1"
                     /translation="MIKSFKSKKLKQFSKGDTSKVAQAHKKKIEIILNALDTATSILDL
                     DLPGLDLHSYAEFTPVRWSLKVSANYRIFFEWDGANVNNVDYDDPH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1179..1292)
                     /locus_tag="MU413_RS27955"
     CDS             complement(1179..1292)
                     /locus_tag="MU413_RS27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_077259703.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPKKHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            1705..1896
                     /locus_tag="MU413_RS27960"
     CDS             1705..1896
                     /locus_tag="MU413_RS27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1896..2297
                     /locus_tag="MU413_RS27965"
     CDS             1896..2297
                     /locus_tag="MU413_RS27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3140..3349)
                     /locus_tag="MU413_RS27970"
     CDS             complement(3140..3349)
                     /locus_tag="MU413_RS27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_009310047.1"
                     /translation="MKSIKTFVAVAALSMMSFGAFAQSVSATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     Typing          complement(1180..1585)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.195"
                     /coverage="99.0"
                     /evalue="4.64e-162"
ORIGIN
        1 aaggttcatg atcttgcatt tcagcggcat ctaggttccg cggagtgtaa atggtttcaa
       61 cttcgtcaag gtttacttta tcctgagcgc ggtataagcc gtacgctatc tgcattttca
      121 tccatgactc ggcggaacga ccgaaagctt ttgataggcg aatagccatc tcgtaggaca
      181 ggtctgcttt ttggttaacc aaccgactga ttgttgatgt atcaaccccg atcttctctg
      241 caaaagtgct tatctgaatc tctaaaggtt ctaaatatac ttcgcggatg aactcgccgg
      301 ggtgcatagg tttttgcaat gtaaacatgc aacctcctgg ttttttgcta tttgcgcctt
      361 gcgcaaagcg caaatctatt tacttttaaa atcatttgtg gtaaaatgat ttagttaagg
      421 gaagtgtttt ttttcttagc ggcaacacac acggttcttc agttgtgttc ttgatgtgtc
      481 tatttgatag ttctaaagct tcgcaggcgt agaatactac ctataaataa gactgagtca
      541 agaacattta ccttgcatga agaacttcta aaaaaaacta tatggagaag ttcaatgatt
      601 aaaagtttta aatccaagaa gctaaagcag ttcagtaaag gcgatacaag taaggtagca
      661 caagcgcata aaaagaaaat agagattatc ttaaacgcgt tagatacagc tacttccatt
      721 cttgatctcg accttcctgg tttagatctt cactcgtatg ctgagttcac tccagttcgc
      781 tggtcactga aggtgtcagc aaactatcgt attttctttg agtgggatgg ggcaaatgtt
      841 aataatgtgg attacgatga cccacattaa tcaataaaat caatgactta ggtcacggtc
      901 ttggttcggt tatccgaacc aagaccgtga ggtctattat atgcgttttt tttattaagt
      961 caatggcttg gtgaggataa tttcttgaga ttgatgttgg tgcagagtat ctttaaacca
     1021 caaacgaaaa aaccacccgg caaggtggtt ttttcgaagg ttcagtaacc ggcaagtttc
     1081 tgaaccaagg taactggctt ggaggagctt agtcaccaaa acttgttctt tcagtttagc
     1141 cttaactgac gcataacttc aagactaact cctctaaatc agttaccagt ggctgctgcc
     1201 agtggcgctt tgtcgtgctt tttcgggttg gactcaagac gatagttacc ggataaggcg
     1261 cagcagtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgcctac
     1321 ccggaatcga gtgtctggcg tggtatgaga aaccgcggcc ataactgcgg aatgacaccg
     1381 ataaaccgaa aggcaggaac aggagagcgc acgagggagc taccaggggg aaacgcctgg
     1441 gatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat tttgtgatgc
     1501 ttgtcagggg ggcggagcct atggaaaaac ggccgccatg cggcctttcc ttttcccctt
     1561 acgcgatcgc tcggttttct cgagctttat acgcatgttc ctgccgctcg ccgcagccga
     1621 acggcaggag cgtagcgact gagtgagcga ggaagcggaa aagagaacat ccctacactg
     1681 ggcactgacg ctctgaggct tcttatgacg aaacaacaga aaacagcgct gaatatggct
     1741 aaattcatcc agaaccagtc tctgctcctg ttggagaagc ttaacgagct tgatctcgat
     1801 gtcgaagccg acctttgtga gaaactccac gatgatgcgg agcatctttt ccgtacactg
     1861 tcctcgagac tggatgcatt acaggatggt aactgatggc cagagtgtct attagtgagg
     1921 ctgcccgact gacgggtaag agtagaacaa cactgcacag gttaattaaa gcaggtgacc
     1981 tgtccacctg tagcggtgaa cgcaatgcaa aaatggtaga tacttcagag cttttacgtg
     2041 tctttggtcc ctttgaacaa ccgaagggtg aacaccattc tggacaggtc tctgaacagc
     2101 gtgatacagc ctctgctgac cagagtgaac aggtgattcg tcatctgaaa caggagattg
     2161 aacacctgaa caccttactt ttgtccaagg attcgcacat tgacagcctg aaacaggcca
     2221 tgctccttct tgaaaatcac caggcaaaat taaccgaatc cccggcgcca tggtggaaat
     2281 tctggaaaaa atcctgaccc cgtcatgctt cagttttcct cttcggccat cccggggagt
     2341 tcctgaacct tcccaaaaat cgtcagatct ctgaacgctc ctcatcacta aaaataccca
     2401 caccccacgc aaaattaaaa ttttgaagat aactcattaa tatcagttag ttgtgggtta
     2461 ttaaattacg cagtattact ttctgtaagg tgaatccaag tcgtcggcga ttcgtgtacg
     2521 acttgccgac gactcgtcat agagtcatcg acgactcact taagactatc agtaacttat
     2581 gacgacttga ctctagagtc gaatcgtcat tttggtgttt ttttgacctt gacgactcta
     2641 gagtcaagtc acgagtcgaa tcgccggtga attgtctggt gagtcgtccg gcgacttgcc
     2701 ataacgtgat tgccggccgt cgacgaaccc gattcctgat cgtgctgaaa accggttttc
     2761 cggctgcgcc ggcaacgctt cacggccacg caataaattg cgtccccgtt caccgttttc
     2821 ggataggaag aacgccggaa acaggactta cccaggctga atggcgtgaa ttttcgcgct
     2881 atgcacttgc gtgcatactc atgcatgccg taaaaacaga gcctgcgcgt ttctggcggg
     2941 ttttcgggtg gtttgttgcc tgttttaccg gtttcccgtc agaaacgccc tgaggccgtt
     3001 ttagcggtgc gtacaatcat gcctttatgt taaatgacag gtgcaaaaaa gcccccgaag
     3061 gggcctgaag gttttcctgc taagggacga aacgggccgc ctcccggcga cctgttcggc
     3121 agctccctct gcgaccatct tatttggtca gttcagccgt catgtggaca cggttattga
     3181 actgagcgct ggtgattttg tatgacgcac cttgttcagc ggcctgtgcg gcaatttttg
     3241 cttcagcacg atccagggtg gaggcggtag cgctgacgct ctgagcaaag gcaccgaaag
     3301 acatcatgga tagagcggca actgcaacga aagttttgat ggatttcatg gtcgtattcc
     3361 ttaagtcatt ttgttctgta tggggcgttg ttgccctgat gtgataaatg ataggacgac
     3421 catgaactga ttaaaatcga aacaatttgc gaatgtcatt caaaaaaata gaataaaaag
     3481 tgagcatata aaagctaagt atgattcttt ggttccaggt ggactgaatg gagagaattg
     3541 aaagtgtggg catgataaat agagagggta taacgaagac agaaaagccc tctatagggc
     3601 ttttctgtct atgcaaaacc atgcgttgct tacagattta tat
//
