LOCUS       NZ_CP095143             4204 bp    DNA     circular CON 03-JUN-2024
DEFINITION  Klebsiella pneumoniae strain Iso00199 plasmid pIso00199_03, complete
            sequence.
ACCESSION   NZ_CP095143
VERSION     NZ_CP095143.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17766581
            Assembly: GCF_022964855.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Pankok,F., Taudien,S., Dekker,D., Thye,T., Oppong,K., Akenten,C.W.,
            Lamshoeft,M., Jaeger,A., Kaase,M., Scheithauer,S., Tanida,K.,
            Frickmann,H., May,J. and Loderstaedt,U.
  TITLE     Microepidemiology of plasmids in Escherichia coli and Klebsiella
            pneumoniae with acquired extended spectrum beta-lactamase genes
            isolated from chronic wounds in Ghana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Pankok,F. and Taudien,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2022) Insitute of hospital hygiene and infection
            control, University Medical Center Goettingen (UMG), Humboldtallee
            34A, Goettingen, Lower Saxony 37073, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 16-FEB-2022
            Assembly Method                   :: Unicycler v. v0.4.8
            Assembly Name                     :: BNI_Kpneum_Iso00199_hybrid
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022964855.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 03:43:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,613
            CDSs (total)                      :: 5,485
            Genes (coding)                    :: 5,358
            CDSs (with protein)               :: 5,358
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 56 of 127
            Pseudo Genes (incomplete)         :: 74 of 127
            Pseudo Genes (internal stop)      :: 15 of 127
            Pseudo Genes (multiple problems)  :: 16 of 127
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095143.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Iso00199"
                     /isolation_source="Chronic wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pIso00199_03"
                     /geo_loc_name="Ghana: Asante Akyem District"
                     /collection_date="2016"
     gene            complement(346..471)
                     /locus_tag="MU413_RS28120"
     CDS             complement(346..471)
                     /locus_tag="MU413_RS28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_105720471.1"
                     /translation="MHEPPVQSDRCALSGNYRLESKPERHDKAPLAAAIGNLVRR"
                     /name="replication initiation protein"
     gene            complement(1638..1919)
                     /locus_tag="MU413_RS27925"
     CDS             complement(1638..1919)
                     /locus_tag="MU413_RS27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_105720469.1"
                     /translation="MKNEDDYKTGWTTQTTNPATGKKCSGGAARNLRIYQAGGANSVRV
                     KAAIEGVQSIQPIIDMQQSQIEQQQAQIAMLTQSLSQAINELTKNGNQ"
                     /name="hypothetical protein"
     gene            complement(1929..2186)
                     /locus_tag="MU413_RS27930"
     CDS             complement(1929..2186)
                     /locus_tag="MU413_RS27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_105720468.1"
                     /translation="MPKKTSKPNDLSSTINNIKKEINSGFTELLNRVEALEASDAQHSM
                     AIRDLQIQARAARGDKRMDIARDFDLSEGRISQIVNAGRN"
                     /name="hypothetical protein"
     gene            2731..3441
                     /locus_tag="MU413_RS27935"
     CDS             2731..3441
                     /locus_tag="MU413_RS27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000967646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_105720467.1"
                     /translation="MLYWKRMPSLWIGIKISEFNDLDTAKAIAALKIYLTFCLFCKESD
                     SGQRTVELTFSNLCEIASLSRSLVNEGLKILYAKELIKNLSTTVRKKIYTVDVLDKHDD
                     GWCKLPFKGIVGEDGKISAFQSMHNRYPFELLALQTYMYLLYARDNRNEYTLARKKTIC
                     KKLKCKLPELNKAITYLIHIGLLDKIVKKSIENKPLEMFHDSCYFYFRAGSNSALTYKR
                     KSEVLNLSEEDLPF"
                     /name="hypothetical protein"
     gene            complement(3552..4067)
                     /locus_tag="MU413_RS27940"
     CDS             complement(3552..4067)
                     /locus_tag="MU413_RS27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
                     /protein_id="WP_105720466.1"
                     /translation="MKFYTISSSYIRYLKGFDDKVPNSEDPTYNNPKAFIGIVLEIEGH
                     KYLAPLTSPKAWHANVKESSPAFFKLHENGVPDNQLGLINLKFMIPIIEAEVSLLDLDS
                     MPNTPYKRMLYKQLQFIRVNEDKISEKSKLLRNLALQGRMQGTCDFAVLEEKYQYFGKK
                     PEDMEIDD"
                     /name="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
     Typing          complement(133..653)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.642"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aatcacctga atttctagct gcacttaaag gtagcaaatc accttgaact aagtatacat
       61 gatcttcgct tgggcttaca actaggtgct tagcatggga tgttcagtca aatgacggca
      121 ctaatcaaaa ggatcttctt gagatcatct ttttctgcgc gtaatctctt ggtctgaaaa
      181 cggaaaaacc acccggcaag gtggtttttt cgaaggttca gtaaaccggc aagtttctga
      241 accgtggtaa ctggctttcg cagagcacag caaccaaatc tgtccttcca gtgtagccgt
      301 actgaggcgc acacttcaag agcaacctcg tgtttagcta acaaatcatc tgcgaaccaa
      361 gttaccaatg gctgctgcca gtggcgcttt gtcgtgtctt tccggtttgg actcaagtcg
      421 atagttaccg gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca tacagtccag
      481 cttggagcga actgccttcc cggaaccgag tgtcaggcgt ggaatgagat aaacgcggcc
      541 ataacagcgg agtgacaccg gtagaccgaa aggcaggaac aggagagcgc acgagggagc
      601 caccaggggg aaacgcctgg gatctttata gtcctgtcgg gtttcgccgc cactgatttg
      661 agcgtcagat tctgtgatgc tcgtcagggg ggcggagcct atggaaaacc ggcaacgccg
      721 cggcctcctc tttttcctct ccatcttctc tcatcgcctg tgcattaatg tctctgcccc
      781 ctccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaata
      841 tctgcgagct tccatcagga acaggacgcg ctgcctttgt ctttcttgat cgcgatgcag
      901 ggtagggaag agaccgcaaa atggcgccat tcgacgctct tgcattacaa aagttaaagc
      961 agcggacagg aaatgttttg tggccacagc cagtttgtcc gtgccaccaa acaagcggga
     1021 caagatgtgt ttttggagta ccgcccggac tccaaaacgc tatgatcagg ctccgcccga
     1081 tacctttagc gacaagtgtt tgccatctgt ctttaatctg tttgctgagt gttttgaatc
     1141 tgtttgctaa gtatttgcaa tgtgttttga atctgtttgc tcagtgttta tagcctgttt
     1201 tctccctgat ttactctgca tgcccaaggc acgttagcga tgtatacatc gccgcgccgc
     1261 aagggtgccc cttccgaatc ccgttcctct ctgtgcgctg atgaatccag gttaaatccg
     1321 accccttggc aataaatcct gccatgactg acggcctggc caacgcttca cgcccgcaca
     1381 gtaaactgcg cacccgttca ccgttatcag gagggttcat gatggctttc aggacgttcg
     1441 acggatttta gccgctaaca ctgcggtcat gcgttcgtgt gcatggctat gcacgcgcgc
     1501 agaaatcatc tggcgcgttt ttcacggtac gggtgggata aagatgccgg aaacgaccat
     1561 ccggccgcag aaacgcgctg agggcctctc aggcggtgcg gccaacggat gttatggtaa
     1621 ataacatccg ttgtagccta ttgattcccg ttttttgtta gttcattgat tgcctgagac
     1681 aaagattgag tcagcattgc tatttgagct tgttgttgct cgatttgtga ctgttgcata
     1741 tcgatgattg gctgtattga ctggacgccc tcgattgcag cttttacccg cacggagttt
     1801 gctccaccgg cttgatatat ccttaagttt ctggctgcgc ctccagaaca ttttttccct
     1861 gttgccgggt ttgttgtctg agtcgtccat cctgttttgt agtcgtcttc atttttcata
     1921 agtctgcctt agttgcgacc cgcattaacg atctggctaa ttcgtccctc gctgaggtca
     1981 aagtctcttg ctatatccat acgtttgtct ccgcgagcgg ctctggcctg aatttgtaaa
     2041 tcacgtatcg ccatgctgtg ttgtgcgtca ctagcctcta aggcttctac gcgattaagt
     2101 aactcagtaa aacctgagtt aatttctttc tttatgttgt tgatagtgct gcttaaatcg
     2161 tttggttttg atgttttttt aggcatggca tatttctcta tactcggtgg ctaaggccgc
     2221 taaagatttt tagttttgtt aaagttgtaa ggtagggatt attgctcttt ttggcaagat
     2281 gaatttaatg agataaagaa atttagtgct ttatacaggg tggcttgcaa ggcacaacgc
     2341 tttgtttgtt cggatctaaa gtcctaatgt gaaaacattt aacgtaacca aaaaagcaac
     2401 agcactgcat ttatggttca ccatgaacca taatattctt tctaagaata ccaaaaacgg
     2461 aataaaattc tttaattaac atacagatat ctttttgttg ccatatttta tctttctaga
     2521 tagatttttg tgaaggcgtt acaacgcctt cacagcctga gataatcaaa aaaacaaagc
     2581 tatcaggagg gagaaaaaat gtttgagaaa ggtgtgtttt tggctgaaag gcttgcgaag
     2641 gtcgcagtgg tgctaactaa tctggtgcta tctctgtctg tcttggggtt ggtctatttc
     2701 atttttcaaa ttctgggaat gaggtaattt atgctgtact ggaaaagaat gccttcacta
     2761 tggataggta tcaaaatatc tgaattcaac gatcttgata cagcaaaagc catcgccgcg
     2821 ttaaagattt atttaacatt ttgccttttc tgcaaagagt cagattcagg acagagaact
     2881 gttgagctaa cttttagtaa cctatgtgag atagcatcat taagccgatc gttagttaac
     2941 gaagggctta aaattcttta tgcaaaagag ttgataaaaa atttatcaac gactgtacgt
     3001 aaaaaaattt ataccgtaga tgtattagat aagcatgatg atggttggtg taagcttccc
     3061 tttaaaggaa ttgtaggtga ggacggaaaa ataagtgcgt ttcaatccat gcacaacaga
     3121 tatccatttg agttgctagc tttacaaacc tacatgtacc ttctttatgc tcgtgataat
     3181 agaaatgagt atactcttgc taggaaaaaa acaatatgta aaaaactgaa atgcaaacta
     3241 cctgaactaa ataaagcgat tacctatctt attcatattg gtttgcttga taagattgtt
     3301 aaaaaatcaa tagaaaacaa gcctttggaa atgtttcatg attcatgtta cttctacttt
     3361 agagcgggaa gtaacagcgc tctcacttac aaaaggaaat cagaggtgct taatctttca
     3421 gaggaggact tgcctttttg attacttctc gaaatcaaaa ttagaatatt ttattcttat
     3481 attggctgag agcagaaaat taggtccaaa accgcttgat tttggacctt caatgccata
     3541 aaaacgaatg gttaatcgtc tatttccatg tcttcaggtt ttttaccgaa atattgatat
     3601 ttttcctcta acacggcaaa gtcgcatgtt ccttgcattc tgccttgcaa ggcaaggttt
     3661 cttaatagtt ttgatttttc agatatttta tcttcattta cacgaataaa ttgtaactgt
     3721 ttatatagca ttcttttgta aggcgtatta ggcatgctat ccagatcaag taaggacact
     3781 tcagcttcga ttattggaat cataaatttc agattaatca atccaagctg attatcaggg
     3841 acaccgtttt catgaagttt gaagaaggca ggtgatgact cttttacatt agcatgccat
     3901 gcttttggcg acgttaaagg tgctaaatat ttatgtcctt caatctctaa tactatgcca
     3961 atgaaagcct taggattgtt gtatgtagga tcttcgctat tgggaacttt gtcatcaaag
     4021 cccttcaggt atcttatgta actgcttgat attgtgtaaa atttcatttt tttctcgtaa
     4081 aaaaggcgac tgtgtatagt cgcctttttt actttattac tttaaggtag taaatgacct
     4141 aaatttctag ctgcacttaa aggtagcaaa tcacctgaat ttctagctgc acttaaaggt
     4201 agca
//
