LOCUS       NZ_CP095136             5835 bp    DNA     circular CON 03-JUN-2024
DEFINITION  Klebsiella pneumoniae strain Iso00267 plasmid pIso00267_04, complete
            sequence.
ACCESSION   NZ_CP095136
VERSION     NZ_CP095136.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17766602
            Assembly: GCF_022964795.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Pankok,F., Taudien,S., Dekker,D., Thye,T., Oppong,K., Akenten,C.W.,
            Lamshoeft,M., Jaeger,A., Kaase,M., Scheithauer,S., Tanida,K.,
            Frickmann,H., May,J. and Loderstaedt,U.
  TITLE     Microepidemiology of plasmids in Escherichia coli and Klebsiella
            pneumoniae with acquired extended spectrum beta-lactamase genes
            isolated from chronic wounds in Ghana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Pankok,F. and Taudien,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2022) Insitute of hospital hygiene and infection
            control, University Medical Center Goettingen (UMG), Humboldtallee
            34A, Goettingen, Lower Saxony 37073, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 16-FEB-2022
            Assembly Method                   :: Unicycler v. v0.4.8
            Assembly Name                     :: BNI_Kpneum_Iso00267_hybrid
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022964795.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 03:43:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,626
            CDSs (total)                      :: 5,501
            Genes (coding)                    :: 5,350
            CDSs (with protein)               :: 5,350
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 88 of 151
            Pseudo Genes (internal stop)      :: 21 of 151
            Pseudo Genes (multiple problems)  :: 22 of 151
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095136.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Iso00267"
                     /isolation_source="Chronic wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pIso00267_04"
                     /geo_loc_name="Ghana: Asante Akyem District"
                     /collection_date="2016"
     gene            complement(join(5594..5835,1..457))
                     /locus_tag="MU415_RS28030"
     CDS             complement(join(5594..5835,1..457))
                     /locus_tag="MU415_RS28030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_245146874.1"
                     /translation="MPVSLPFCSRIALLMLCELDCSSALIDVSSLANSRFTLCSVSCAC
                     CFDFSSKPFAKFKISLINHSFQPDISAAVRIGNADAAFRFPRHGQAIAFQLRHHVLPAA
                     DHALLHQVHQPGGNFLCRLLFVAGQIAGLRQRAAISRVVRVAQPQPVVGQGLPGVDAGA
                     VGAVKVGLQAFSALQFDVRDDKIQLKPSFVAVLHPQAGVLVAVEARQQRMLPFVHQARF
                     LRVCNVRFLE"
                     /name="hypothetical protein"
     gene            456..758
                     /locus_tag="MU415_RS28035"
     CDS             456..758
                     /locus_tag="MU415_RS28035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_245146875.1"
                     /translation="MLRASVSTAMAWIFICFALLIGILSGIIWFQGTIITSRLSEMDSY
                     SQQLESLKAKSGAGVAIYSDDKHRNTYLVVLPKQAHGVETYTSTTDNTVVKYTAK"
                     /name="hypothetical protein"
     gene            828..1052
                     /locus_tag="MU415_RS28040"
     CDS             828..1052
                     /locus_tag="MU415_RS28040"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_226939304.1"
                     /translation="MEKQLLSALESLQTSYEQQQQAWQDSYSSLQHMFEATSQALTHSD
                     RVCQHLSSQVESLRAQVESLSRNVSRLMR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1214..1546
                     /locus_tag="MU415_RS28045"
     CDS             1214..1546
                     /locus_tag="MU415_RS28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_094966193.1"
                     /translation="MSHAIEFIETPMFTRQIKQIATDEELRELQKTLIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSLTDAEKAALKTLTQ
                     QLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1548..1832
                     /gene="nadS"
                     /locus_tag="MU415_RS28050"
     CDS             1548..1832
                     /gene="nadS"
                     /locus_tag="MU415_RS28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_001697687.1"
                     /translation="MSFFDELKASLEEAVEIKNGVREPARVTRYELADVKAIRAQLNVS
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELAAH"
                     /name="nadS"
     gene            2025..2954
                     /locus_tag="MU415_RS28055"
     CDS             2025..2954
                     /locus_tag="MU415_RS28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_211661996.1"
                     /translation="MHNAALALFNDRLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGAAIDWSDRNAPAPTLTITNPDNGHAHLLYALKTSIRTAPDGKIK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHGHWKIGVWQPELYTLDWLADFLELNAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYDRWLQACYERASAYNLQFAAPLD
                     ENEVSGVSKSIAKWTFSKFTQSSFISYVERTHTSDIQSRRGQKGGKVSSGGGRSIASNS
                     ERQLKPWEDMSISRATFYRRRKNGNSII"
                     /name="replication initiation protein"
     gene            2935..4827
                     /locus_tag="MU415_RS28060"
     CDS             2935..4827
                     /locus_tag="MU415_RS28060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000407093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_245146873.1"
                     /translation="MEIVSFKVSQYRSISGEISSDNFNGLTIVGQNNSGKTNLLKAMRV
                     FFEGKFNSDLYDYEKDKPHGVKQGQTSFAVRFTFSESEINDVSEDLSQDDDDDDDDDSV
                     IDLGVLGEQEELGDSISLDDGGTKQQETKPTQSRGRKLSLRDRYLQLFNMIDDSRLNSP
                     QNEVTVHLTISNKNNYFYTVFKGYKRKPEVTSSMYTSVERRFIEKILSSFECVYIPSSK
                     SIDDIYHNLLVPYVRSEVASAMKTSVQVIHDKLLEVSEKITNEMTLSGIDDCSFMIKAP
                     PSLDELFGRFEVSIKDTIENVISSKGMGLQCLSLFSSFKSISERKNSLGKKTIWMVEEP
                     ESFLHPSLGRSCNHIFNSLMDVAYVLITTHSLSFVSDEPSKIIELYKDDAGVTKDKKHE
                     KRFNAVASIRENLGVRFSDFFNISSSAVFVEGITDKQYIESILKLTYESDDFKDRWPFL
                     RSAKVDEFGGVSQLGGFLKGCYEFISKEVPVISIFDGDEAGVKERTRLQSYFGKKQVRF
                     ESNKDYISVRSGFAIEGLFPDDFISDAMETHPSWFIGGKSVDADDVIEPFKVQDNKKTN
                     LSNFFLEKCRVQPICDWISRWEKVFNVIDSALRDKSESITDKKRTEDTSDNQAA"
                     /name="AAA family ATPase"
     gene            5104..5430
                     /locus_tag="MU415_RS28065"
     CDS             5104..5430
                     /locus_tag="MU415_RS28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_064161982.1"
                     /translation="MEKRTKEIKIRLTETEHQRLLDRCDRTHLAEWLRQLGLGEHTSRK
                     RRVPDVAPELLRQVSGIGNNLNQIARRLNQTDTLTPSERVSLLVVLTSLDRQLGDLLEQ
                     NRDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(5238..5430)
                     /name="Col(Ye4449)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.564"
                     /coverage="100.0"
                     /evalue="3.99e-40"
     Typing          3..1150
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.956"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5421..5833
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.101"
                     /coverage="99.0"
                     /evalue="1.58e-80"
ORIGIN
        1 caacgaccgg ctggggctgc gcgacccgaa cgacccgact tatcgccgcc cgctgacgca
       61 ggccagcgat ctgccccgcg acaaacagca ggcggcagag aaaattaccg ccgggctgat
      121 gaacctgatg cagcagggcg tgatccgcag ccggcaggac gtggtgacgc agctggaaag
      181 ctatggcctg accgtggcgc gggaaacgaa aagcagcatc agcattgccg atccggacgg
      241 cggccgaaat atccggctga aaggaatgat ttatgagcga gattttaaat ttggcgaagg
      301 gcttcgagga gaaatcgaag cagcaagcgc aggataccga gcagagcgtg aagcgcgagt
      361 tcgcgaggct ggagacgtct atcagcgcgg aactgcaatc aagctcgcag agcatcagca
      421 acgctatccg cgagcagaac ggcagactga cgggcatgct cagagcctct gtctcaacgg
      481 cgatggcgtg gatattcatc tgcttcgccc tgctgatcgg catcctgagc gggataatct
      541 ggtttcaggg cacgatcatc acctcgcgcc tgagcgaaat ggacagctac agccagcagc
      601 tggagagcct gaaagcgaaa agcggcgcgg gcgtggcgat ctacagcgac gacaagcacc
      661 ggaacaccta tctggtggtg ctgccgaagc aggcgcacgg cgtggaaacc tatacatcga
      721 cgaccgacaa caccgtagtg aaatacacgg cgaaataacc ccgtcagagc gccctgaaac
      781 gacacagcgc gtttttaaca ccgaggatga ccaaaaccat gaccgagatg gaaaaacagc
      841 ttctgagcgc cttagagagc ttacagacaa gctacgagca acagcagcag gcgtggcagg
      901 acagctacag cagcttgcag cacatgttcg aggctacttc acaggcgctg acgcacagcg
      961 accgggtgtg tcagcacttg agcagtcagg tagagagctt gcgcgcgcag gtcgagagct
     1021 tgagcaggaa cgtcagccgc ttgatgcgtt gatcaggcag cacgaccgcg cctacgccga
     1081 gaagctgcgt gagcagcagc cagagccgga gccgcagaaa aaaacgactc gttactatgg
     1141 cccctcgatg tgaccgcttt tccgaaatca gaattaagtg tactatgtac tccgttaaaa
     1201 acaggagaca gccgtgagtc acgcgattga gtttatcgag accccgatgt ttacccgtca
     1261 gataaaacag atcgccacgg atgaagagct cagggagctg caaaaaacgc tgattgagtc
     1321 gccggacaaa ggcgatctca tccagaaaac cggcgggctg cgaaaaatca gaatggcgac
     1381 gggaaaccag ggaaaaagcg gcagcgcgag ggtgatttac ttcctggcca ccgccgaggt
     1441 gatttatctg gtgatggcct acccgaaaag caccaaagac agcctgacgg acgcggagaa
     1501 agccgcgctg aagacgctga cccaacaact gaaagacgag gtgtgacatg agtttttttg
     1561 acgagctgaa agcatcgctg gaagaagccg ttgaaatcaa aaacggcgtc agagagcctg
     1621 cccgcgtcac ccgttacgag ctggcagacg tgaaggccat cagggcacag ctgaacgttt
     1681 cacagagcga gatggcaaaa gtgctgggaa ccagcctcga caccatcaag agctgggaaa
     1741 ccggacgccg taacccgacc ggactggcgg cgaaggtgct ggcaaccatc caggcgaacc
     1801 cgaaattctt ccaggagctg gcggcgcact aagccgttgt gacagaagga ttcgacagac
     1861 gaggccgttc gcgtgtaatt taacggcaac agaaaaaaca aagccccggc aatcatgagc
     1921 tatcttggcg gatgagcatg actaccaggg ccaggtcgaa accttgaaag gatattagca
     1981 catgaaccgt gccagacaac agcccgccat aagcgcaggg cacattgcat aacgccgcac
     2041 tcgcactctt caacgaccgc ttaccgcaca agccgtactt ttcggacgat ctgcattttg
     2101 gcgtacgcat tgccggtaag gaacgcgcca ttctggcgaa atacatccag ttcaatcagc
     2161 ctcacgccat gttctggctg ggattcgacg tcgatcgcgc cggtgccgcc atcgactgga
     2221 gcgaccgcaa cgcacccgcg cccacgctga ccatcaccaa ccccgacaac gggcacgcgc
     2281 atctgctgta cgcgctcaaa acctcgatac gcaccgcgcc ggacggaaaa attaaaccac
     2341 tgcgctacgc cgccgccgtg gagaacgcgc tacgtaaaaa attagaggcc gacgcggggt
     2401 attcggggct aatctgcaaa aatccgaacc acgggcactg gaaaatcggc gtatggcagc
     2461 cggaactcta cacgctggac tggctggctg attttctcga gctgaacgct gcgaacgaca
     2521 aagaaatcgt tgccgactac ggcttaggcc gtaactgcac tctgttcgac aaaacccgca
     2581 aatgggcata ccgcgctatc cgtcagggct ggcctgagta tgaccggtgg ctgcaagcct
     2641 gctatgaacg agccagcgcc tacaacctgc aatttgcagc gcctctggat gaaaatgagg
     2701 ttagtggagt ttcaaaaagt atagctaaat ggactttttc aaaattcact caaagctctt
     2761 ttatttctta tgttgaaaga actcatacga gtgacattca atcgcgtagg ggccagaaag
     2821 gcgggaaagt gagttccggt ggaggaaggt caattgcttc aaatagcgaa agacagttaa
     2881 agccttggga agacatgtcg ataagtcgcg ctacattcta tcgtaggagg aaaaatggaa
     2941 atagtatcat ttaaagtatc tcagtatagg tctatttcag gagagatatc ctctgataac
     3001 tttaatggat taaccattgt tgggcagaac aatagtggta agacaaatct attaaaagcc
     3061 atgagggttt tttttgaagg taaatttaat tctgatctat atgactatga aaaagataag
     3121 ccacatggtg ttaagcaagg acagaccagt tttgctgtaa gatttacttt ttctgaaagt
     3181 gagattaatg atgtttctga ggatttgagc caagatgatg atgatgatga tgatgatgac
     3241 tcggttattg atcttggtgt tttaggagag caagaagaac ttggagattc tatttcactt
     3301 gatgatggag gcactaaaca acaagaaaca aagccaactc aatcaagagg aaggaagcta
     3361 agtttgagag ataggtattt gcaactgttt aacatgattg atgactctag gttaaattca
     3421 cctcaaaatg aagtgaccgt gcacttaaca atatctaata aaaataatta tttttatact
     3481 gtttttaaag gttacaagag gaagcccgaa gtcacttcct ccatgtatac atctgttgaa
     3541 agaagattca tagaaaaaat actttctagt tttgaatgtg tatacatacc atcctcaaaa
     3601 agtattgacg acatatatca taatctatta gtgccatatg ttaggagtga agtcgcttca
     3661 gccatgaaaa catcggttca ggtgattcat gataagctgc tggaagtgtc ggaaaagata
     3721 acaaatgaaa tgactctaag tggtatagat gactgtagtt ttatgattaa agctccacca
     3781 tctctggatg agttatttgg tcgctttgag gtttctataa aagatactat tgagaatgtg
     3841 atctcgagta aaggtatggg gctgcaatgc ttatctttat tttcttcatt taaatccatt
     3901 tcagagagaa aaaattcgct aggtaagaaa acgatttgga tggttgaaga gcctgagtct
     3961 ttcttacatc catcattagg gcggtcatgt aatcacatat tcaattcatt gatggatgtt
     4021 gcttatgttc ttattacaac tcattcctta tcatttgtca gtgatgagcc aagcaagata
     4081 atcgagctat ataaagatga tgccggtgtt acaaaagata aaaagcatga gaaaagattt
     4141 aatgcagttg caagcataag agaaaatctt ggggtcagat tttctgattt ctttaacata
     4201 tcaagcagtg cagtttttgt tgaaggtatc accgacaagc aatatataga gtcaatattg
     4261 aagttaacgt atgaaagtga tgactttaag gataggtggc ctttcctgag aagtgctaaa
     4321 gttgatgaat ttggcggtgt cagtcaactg ggcgggttct taaagggttg ctacgagttt
     4381 atttctaaag aagtccctgt gatatccatt tttgacggtg atgaagctgg ggttaaagaa
     4441 aggactagat tgcaaagtta ctttggtaaa aagcaagtta gatttgagtc aaataaagat
     4501 tatatctctg taagaagtgg ttttgcaatt gaaggcctct tccctgacga tttcatttct
     4561 gacgcaatgg aaacccatcc gtcgtggttt atcggaggaa aatctgttga tgccgatgat
     4621 gtaattgaac cattcaaggt gcaagataat aaaaaaacta acttatcaaa tttcttcttg
     4681 gaaaaatgtc gcgttcaacc tatatgcgat tggatttctc gatgggaaaa ggtgtttaac
     4741 gtcatagact cagccttgag agataaaagc gaatccatca cagacaaaaa gcgaaccgaa
     4801 gatacctcag ataatcaagc tgcataatcg attgcgagcg ccgaggctgc acatgcacct
     4861 gattaatcaa gagtgagacc ataacaagcc tatatcagat aacagccctc tgctaagagg
     4921 gctttttttt tgcagccagc accgaatctg acataatgca tagtacttta agccagtaca
     4981 cacagcgtcc cctctaatga ggagcccccg tgactggttc agggggctcg ccgccccccg
     5041 aaacccctgg catccactgc gaaaaattct cacggtggtg cgaatttttt ctcagcggag
     5101 cctatggaaa agcgaacgaa agagatcaaa atccggctca ccgaaaccga gcatcagcga
     5161 ctgcttgatc gctgcgaccg gactcacctt gcggagtggc tgcgtcagct tggtctgggt
     5221 gaacacactt cacgtaagcg ccgtgtaccc gatgtggcgc ccgaactgct gcgacaggtc
     5281 agcgggattg gtaacaacct gaaccagatc gcccgccgtt tgaaccagac tgacaccctg
     5341 acgccttctg aacgtgtctc gctgctcgtc gtactgacca gccttgaccg tcagcttggc
     5401 gacctgctgg agcagaaccg tgatcgttaa aattcactca cggggtgcgg gttcgggcag
     5461 cgggccggtg gattacctgc tgggaaaaga ccggcagcgc gaacaggcca gcgtgctgcg
     5521 cggcaatcct gagcacgtcc gcgagctgat cgacggctgc gactttgccc gcgcctacac
     5581 ttcaggcgtg ctgtcattcc aggaacctga cattgcagac gcggagaaat cgcgcctgat
     5641 ggacgaatgg gagcatacgc tgttgcctgg cctcgaccgc gaccagtacg cctgcctgtg
     5701 ggtggagcac cgcgacaaag gacggcttga gctgaatttt gtcatcccga acatcgaatt
     5761 gcagagcgga aaacgcctgc aaccctactt tgaccgcgcc gaccgccccc gcgtcaacgc
     5821 ctggcagacc ctgac
//
