LOCUS       NZ_CP095135             9294 bp    DNA     circular CON 03-JUN-2024
DEFINITION  Klebsiella pneumoniae strain Iso00267 plasmid pIso00267_03, complete
            sequence.
ACCESSION   NZ_CP095135
VERSION     NZ_CP095135.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17766602
            Assembly: GCF_022964795.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Pankok,F., Taudien,S., Dekker,D., Thye,T., Oppong,K., Akenten,C.W.,
            Lamshoeft,M., Jaeger,A., Kaase,M., Scheithauer,S., Tanida,K.,
            Frickmann,H., May,J. and Loderstaedt,U.
  TITLE     Microepidemiology of plasmids in Escherichia coli and Klebsiella
            pneumoniae with acquired extended spectrum beta-lactamase genes
            isolated from chronic wounds in Ghana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Pankok,F. and Taudien,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2022) Insitute of hospital hygiene and infection
            control, University Medical Center Goettingen (UMG), Humboldtallee
            34A, Goettingen, Lower Saxony 37073, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 16-FEB-2022
            Assembly Method                   :: Unicycler v. v0.4.8
            Assembly Name                     :: BNI_Kpneum_Iso00267_hybrid
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022964795.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 03:43:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,626
            CDSs (total)                      :: 5,501
            Genes (coding)                    :: 5,350
            CDSs (with protein)               :: 5,350
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 88 of 151
            Pseudo Genes (internal stop)      :: 21 of 151
            Pseudo Genes (multiple problems)  :: 22 of 151
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095135.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Iso00267"
                     /isolation_source="Chronic wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pIso00267_03"
                     /geo_loc_name="Ghana: Asante Akyem District"
                     /collection_date="2016"
     gene            join(7342..9294,1..12)
                     /locus_tag="MU415_RS27975"
     CDS             join(7342..9294,1..12)
                     /locus_tag="MU415_RS27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            12..743
                     /locus_tag="MU415_RS27980"
     CDS             12..743
                     /locus_tag="MU415_RS27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            750..1280
                     /locus_tag="MU415_RS27985"
     CDS             750..1280
                     /locus_tag="MU415_RS27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1308..1487)
                     /locus_tag="MU415_RS27990"
     CDS             complement(1308..1487)
                     /locus_tag="MU415_RS27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1556..1933
                     /locus_tag="MU415_RS27995"
     CDS             1556..1933
                     /locus_tag="MU415_RS27995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(1995..2294)
                     /locus_tag="MU415_RS28000"
     CDS             complement(1995..2294)
                     /locus_tag="MU415_RS28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2284..2547)
                     /locus_tag="MU415_RS28005"
     CDS             complement(2284..2547)
                     /locus_tag="MU415_RS28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(2853..3272)
                     /locus_tag="MU415_RS28010"
     CDS             complement(2853..3272)
                     /locus_tag="MU415_RS28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            3675..5360
                     /locus_tag="MU415_RS28015"
     CDS             3675..5360
                     /locus_tag="MU415_RS28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5370..5627
                     /locus_tag="MU415_RS28020"
     CDS             5370..5627
                     /locus_tag="MU415_RS28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            5712..5861
                     /locus_tag="MU415_RS28025"
     CDS             5712..5861
                     /locus_tag="MU415_RS28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5965..6543)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          13..740
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.4e-150"
ORIGIN
        1 gggatttact gatggcgctg gagctgtaca acgtcagcca cgcgaaacga caggcgcgga
       61 acgcggagaa aacccggctg accctgcgct ggttgcggga agagctgtgt tccacggcgg
      121 agctggttgc gcggcggctg gggattgcgg cggtgcagcc ggtctaccgc ttcctcgaca
      181 gcctggtggc gaaagggctg ctggtccgga ccaaatatcc ggtggacggc cgtcaggtca
      241 gcgtgtgggg gctgacgccg catggcgtgg cgttctcgtt cgacgaggac gaaccgctgg
      301 cggatgttat tccgtttcag ccgtcaaggg tgtcggcagc acatctgccg caccggctgg
      361 ccgtgcaatc cctgcgcctt gatatggaag ccagaggggc caccggctgg cgcattctgc
      421 accgtctgag tcttaaaggc atgaaagtgc cggacgccct ggcggagctg gacggtaaaa
      481 cggtggcgtt tgaggtggag cgcaccgtca agagccgtcg ccggtatcag gaagtggtgt
      541 ccgggtatct gttcaaccgc aaagctaacg gcatcgatga aatctggtac atctgcccgg
      601 accgggcgac gcaggtcagg gtgcagcggg caatcctgtc ggtggatgaa atcgtgaacc
      661 cacagaccgg cgaggcccgc aaaacggcag agctggatcg tgaacggttg tttgcgtgct
      721 ttaaatttat gacaacggag tgagggatta tggcgctgta catggtattt attgaagacg
      781 cggaggaccg gaacggcgag cgctgcatca tgccgaccag cgagcatctg gaagatatgg
      841 tggtgctcgc ctggcagacc ggggcgtatg ttttctgttt tgtcgaccgg gcccagcatc
      901 tggcgatgat gaaatccatc gggatgtatg acgagcggct caacagcgac gaaaaagcca
      961 cgtacttttt cactggtaaa aatccgcaat gggaacggaa caaacccaca ggaatgaagc
     1021 tgtgtgatta cctctatcgc aaggtggagc ggttcgccat cgagaacggt gtgcaggtgg
     1081 ttgatgccgg gaagtgctca ccggagcaga ttaacgcgat gattgacgtg gtgagtgagc
     1141 ggcacgggga aagcgcggtg aacgagaagc tgaaagcgga gctgatgcgg gaatataaca
     1201 gccgctattt taacgtgccg gtacctgaac tgccggagga caaacctgcc cgccggtggt
     1261 ggccgttcgg caaaaaataa atgccagcac cggccatgcc ggtgctgtta ttcctccccg
     1321 agcttatccc gcatcacctg ccagagtgtt tccgcatcct cgtgtagccg ctcgcacatg
     1381 tcggccgggg tatccatatc cagctcattc agtttatcga gcaatagcag cgactgctcc
     1441 ttaataaatt tagccatatt gagggctgtt ttttcctgtt ttgtcatgat tctttccttc
     1501 cgggcatggt gtgcgcctgt caggtatact gtctcaaaaa tagcggggtg agacaatggc
     1561 gtgggtgacg gtgagacagg cgacggaact gacgggtaaa gcccgaagtt ccctgtatcg
     1621 ggatatggcg aaaggccgcg tatcataccg tacggaagct gatggcggcc gggtggtgga
     1681 taccagcgag cttatccggg tgtacggtga gttaagacag gatgagactc acgagcggga
     1741 cgagttgaga ctgtcgggtg agactgaaaa aatggcctcc gacgcgttga tcgcggagat
     1801 aaaagcactc agggaggagg tggccgggct acggcaggaa atgcagtcca tgagactgct
     1861 ggaacacaaa ccggagacag cagagacccc ggccggaaca ccgcgccggt ggtggccgtg
     1921 gggaaaacgc tgacgcgctg tgcttgtcca accggcgacg gccacgcaat gaattgcgtc
     1981 cccgtcaccg gttattagag aacaatatgg ggattctggc gctggccgac cacggccaga
     2041 acccggactt ttctggtctg gtcatcaaca tcatagagga catgctgtcc catcagactg
     2101 atccggcgaa cgtcctcgcc atagcgtgag tccacttcat agagtcgcgg gaagtcggcc
     2161 agtttgtccg ccgcagcgag aacccggtcg gtaacgttct gcgcatccca cagggatgaa
     2221 ttttccgcga tatagtcaaa cagcgtcgtt acatcctcga tggctccctg cttccattcc
     2281 acgtcatacg gcatcgcgca gttcctgata acggccatgc aggcgctggc ggagttgttc
     2341 cgtggtcata cccggacgcg tgtcagcacg ggcggcggtc actttggcgc gaacctgatc
     2401 ttcatactct gcctgggcca gttcttcgtt agccagtcgc tgcaacagcg cagctttgtc
     2461 cgggccacct tccggcagta tgcggggcag gctttcagcc agttcccgaa gaaagttggc
     2521 gttttcgtag tgtgtagtgt tgctcattgc ggagcctccc gggttaatgc ggcaatatga
     2581 aggaagttta gcagggggcg gtctgaaaga atatggtcgt gtcagtcagg ctgatgaagc
     2641 agaaagtcgg taaaaaaggg cttacggcat ccattttacg tcaaaacata tgccatgcag
     2701 ttgcgtgcat aggcatgcat taggataaaa tttatcgggc gcgtttccgg cagttttccg
     2761 ggggggttgt tgcctgtttt atcccgtagc cgccggaaac gccctgagcc cgtctgagcg
     2821 gtgcgcgtaa tgacgcgtta tggtaaatcc acttactgtc tgccttcata gccatcgaga
     2881 taaacagctg aaatggtgtc agccagtagc cttgattttt tcggtgtgag gtctttgctg
     2941 cggtaatcgg tacccgcaaa ttgtgttgtg ccttggagtg agtttaaaaa ttcctgactg
     3001 tgaaactgct gaatgcgttg ctgcacatca ctccgtgatt ttccggtcgc tttatccttt
     3061 gcgcccatat cccagaactg ccggaaaaaa ggcaccatca ttcgtgttgt gtcagctttc
     3121 tgggtggaaa aattgggatc aaaaccgtca tctgaagcat atacgtaatg ccgggtatgc
     3181 tgttccacgc tgccctttgg agcacaggca gtgagagttg ctgtcgctgt tatcatggcg
     3241 ataagacaca gtttgctgcc ggaacgtctc atgagcgtct ccttaccttt aattcttttt
     3301 tctcattgag ttatatatcc gcaggctatc ttatcatcag tttctgaccg tgttcagtga
     3361 ccgaacaacc cgaaccagac cccccgtttc ttttcctgtc ccggaggggg ctttctggta
     3421 tctcatataa tgatttcagt aaaacatact gaggccggag aacggcgccg tttgtgctgg
     3481 tttttgttgc atctggaggg gattgcgcat ggtgtgagtg gctgtttttt tgatcatttt
     3541 cacaaaaacg caaatttaca gacttgacat gaaaaacaag gcagcgtaat ttttgtactg
     3601 tatataaaac cagtggttat atgtacagta ttttatttta gtttattgtt ttaaaagtca
     3661 aagaggaaaa gtatatgagt ggcggagacg gacgaggtcc gggtaattca ggtctgggac
     3721 acaatggtgg tcaggccagt gggaatgtga acggtacatc aggtaaaggt ggcccttcat
     3781 caggtggtgg tacggatcca aacagcgggc cgggctgggg tacgacgcat acacctaacg
     3841 gggatattca taactataac ccgggggagt ttggtaacgg cgggagtaaa cccggtggta
     3901 atggcggtaa cagcggcaat catagcggga gctctggtgg cggacagtct tcggccaccg
     3961 cgatggcctt cggtctgcct gccctggcca ctccgggggc tgaaggactg gctttatccg
     4021 tttccggcga tgcgttgtcg gccgctgttg ctgatgtgct ggctgccctg aaaggtccgt
     4081 ttaagtttgg tctgtggggg attgcgattt acggagtgct gccttctgag atagcaaaag
     4141 atgatccgaa aatgatgtca aaaatcgtga cgtcattgcc ggcagacacg gtaacggaga
     4201 cgccggtcag ctccctgccg ctggaccagg cgacggtcag cgtcactaaa cgtgtggcgg
     4261 atattgtgaa ggacgagcgg cagcatattg cggttgtcac cggccggcca atgagtgttc
     4321 ctgtggtgga tgcgaaaccg acaaaacgtc cgggggtatt cagtgtgtcg attccgggtc
     4381 tcccgtctct gcaggtgagc gtacctaaag gtgttccggc cgcgaaagcc ccgccaaaag
     4441 gcattattgc tgaaaaaggt gattcacgtc cggctggttt tacggccggt ggtaactccc
     4501 gtgaggccgt tattcgtttc ccgaaagaga gcggacagaa accggtctat gtgtcggtga
     4561 cggatgttct tactccggca caggtaaaac agcgtctgga ggaagaaaag cgtcgccagc
     4621 aggcatggga cgctgctcac ccggaagagg ggctgaaaag agagtatgat aaagcgaaag
     4681 ctgagctgga tgctgaagat aaaaatattg cgaccttaaa cagtcgcatt gcatcgacag
     4741 agaaggcgat ccccggtgca agggctgctg ttcaggaagc cgataaaaag gtaaaagagg
     4801 cagaggcaaa taaagatgat tttgtgactt ataacccgcc tcatgaatat ggctccgggt
     4861 ggcaggatca ggttcgctat cttgataagg atattcagaa tcagaatgag aaattaaaag
     4921 ctgcccagac atctttaaac gaaatgaatg aatccttatc cagggataag gctgcgcttt
     4981 ccggggcgat ggagagccgg aaacaaaagg agaaaaaagc gaaggatgca gaaaataaat
     5041 taaatgagga aaagaaaaaa cctcgtaagg gaactaaaga ttacggccat gattattttc
     5101 ctgatcccaa gactgaagat attaaggggt tgggagagtt gaaagagggt aaacctaaaa
     5161 cccctaaaca aggtggtggt ggtaagcggg cccgatggta tggtgataaa aagcgtaaaa
     5221 tttatgaatg ggattcccag cacggtgagc ttgaagggta ccgcgccagt gatggcgaac
     5281 acctcggggc attcgatcca aaaacgggta agcaggttaa agggccggat ccaaaacgaa
     5341 acattaaaaa atatctttaa gaggtaaata tggggcttaa attacatatt cattggtttg
     5401 ataagaaaac cgaagagttt aaaggtggtg aatactcaaa agacttcggt gatgatggtt
     5461 ctgtcattga aagtctgggg atgcctttaa aggataatat taataatggt tggtttgatg
     5521 ttgaaaaatc atgggtttcg atattacagc cacactttaa aaatgtaatc gatattagta
     5581 aatttgatta ctttgtatcc tttgtttacc gggatggtaa ctggtaagca aaagttatca
     5641 aggatgattt gaaatacaga cttccggtgt tcagggatga ataccggggt ttttaaatac
     5701 agggaaggta tatgaaaaag gtaaaggttg tttttctttt tatattgatt ttttcaggct
     5761 ttcttctggt cgcatgtcag gcaaactata tccgggatgt tcagggtgga acggtggcac
     5821 catcgtcctc ctctgaactg acggggatcg cggttcagta gaaaagatca aaggatcttc
     5881 ttgagatcct ttttttctgc gcgtaatctt ttgccctgta aacgaaaaaa ccacctgggg
     5941 aggtggtttg atcgaaggtt aagtcagttg gggaactgct taaccgtggt aactggcttt
     6001 cgcagagcac agcaaccaaa tctgtccttc cagtgtagcc ggactttggc gcacacttca
     6061 agagcaaccg cgtgtttagc taaacaaatc ctctgcgaac tcccagttac caatggctgc
     6121 tgccagtggc gttttgcgtg cttttccggg ttggactcaa gtgaacagtt accggataag
     6181 gcgcagcagt cgggctgaac ggggggttct tgcttacagc ccagcttgga gcgaacgacc
     6241 tacaccgagc cgagatacca gagtgtgagc tatgagaaag cgccacactt cccgcaaggg
     6301 agaaaggcgg aacaggtatc cgggaaacgg cagggttgga acaggagagc gcaagaggga
     6361 gcgattcatc ggaaacggtg atgatcttta agtcctgtcg ggtttcgcca ctcctgactg
     6421 attcatggtt gagccaccgg ctcccacaga tgcaccgaaa aagcgtctgt ttatgtgaac
     6481 tcagtcagga gggcggagcc tatggaaaaa cgccacctgc gtggccttgc cgttttgcct
     6541 cacatgttag tcccctgctt attcacggaa tctgtgggta actttgcatg tgtccgcagc
     6601 gcccgccgca gtctcacggc cggagcgtag cgaccgagtg agcgaggagg cgcaatttcc
     6661 ccggcaccta caagacggac accggacgcg ctgatgtggc atattgtccc ctgattaagt
     6721 ctttatattc cctgataata aattaccagg ataggtgaat catatcggtt tttcgctgaa
     6781 cattttacgt taacctatct ctgttgcttt tttctcctta cggagaaata caggtcatcc
     6841 ccgcgagggc gtggctgacc tcagggggat atcccctgct tacgctggct ggacgccacc
     6901 gacgcacggt ctctccccct ctttctggct ccgtccggct ggacgccggc cacgccagaa
     6961 attcacccgg cccccgtcag gctcacgcct gaccacccac aaaaatccac ggtgctggac
     7021 gcacctggcg aagcagcaaa gctgctcctg attttgcggg tggtcagccg ctcgctatgg
     7081 cgaactctga cgggggccgg aacaccggaa aagaccgcac tggggtgccg ccttttcctc
     7141 tcacacgctg gggcgtgctc gttgttcctc cggggctgcg ccttttccgc atggggcgga
     7201 aagaggctgg cctccgtggt gaaagctgaa cgcaatcacc acggacgtga ccgccgccgc
     7261 catgccttgg ggcatgtccg cggcttgtgc agcgggggct gcacatcacg ccggcaaacc
     7321 atcgtctgaa ggagagagaa catgtttaat acggattcgc ttgcctggaa gtggcacatt
     7381 ccggagctgg cccggcagat ttatccgtgg gtcagctggc tgtaccagga gtattacggt
     7441 ctggccgggg ctttcctagt tatcggcctg tttttcggtc tggctgaact ttgggccgga
     7501 cggatgcgtt ttgtggggct gagtcataag gagcgacggg agtatgccga tcgctataag
     7561 cgcgataata aacggccagc gccattttcg gtgctggcct cgctggattc gcccgggcgg
     7621 atggtcaggg cacgtcgaca tgtctggaac tacgcgtact ggctgaccgg cgcggccggt
     7681 ctggcccttc tgggggggct ggttgtcgcc gtgtttatcg agagctgggg agaaaccgtc
     7741 ctgcgtccgg acctctacgc aaacgcagat tattacctgc tgcagccggc taacctgttc
     7801 gggattgcct ggtgcgcggg cgggctggct gtcgggattg tgttggcgcg gctgctggcg
     7861 ttcctgctgc ttgacggtca ttttgcggct gctgacgagg cggtgaacgc gaaactgaat
     7921 caggagagcc ggcgcagcag ccagcggaca ggagagatga ccgacgtgcg ccacctgcat
     7981 ttcggggagc cggtaccggt taatgcgctg gccgattttt cctcggagca ggcccggcag
     8041 gagcaggcgg tgtttctggg taaggatgag cagggcaagc cggtgctggt tccgcgtgat
     8101 acctggcgca agaccaacat tcagatcctc ggcctgcccg ggagcggcaa gagcgtgatg
     8161 gccaccaacg cgctcattcg ctgcgtacgc gatttcgggg acgcggtggt gtacttcgac
     8221 cccaaagggg acgcatgggc gccgcacgtc ttccgggcgc actgtccgga cttcaccctg
     8281 ctggatctgc ggccggggaa accggcgcag ctgaatctgt tccgcgatct cgaccagtac
     8341 gcgctgaaaa acctgctgat tgccgggctt aacctgggcg agaccggcga cgtggcggac
     8401 cactaccgca tcagcgagca gaaagcggcg aagctgattg cggagcagtt tccgcagggg
     8461 gcaaacattc agcaggtgct ggcggcggcg tacgcgctgc cggaggccct gaaaaaggac
     8521 gtgaaggggt tgatcactag gctggaaaac gtcgctgacc tgagcgtcct gcagacggac
     8581 agtggcatcg atgtggcggg gattatcaac ggcggcggct gcctgtacgt tatcggctcg
     8641 atggacgacg aggcggtgat ccgtgtgcaa aaaatgctgt ttgcccgctg cgcgcaaatc
     8701 atcattgcgc gggacgagtt ccgggagtgg ccacacgtca gcgtgatgct cgatgaaatt
     8761 aaatatctgc tgtcgaagta tgtactgaac gcactgggga cgctgcgcag ccgggactgc
     8821 aatctgttgc tcgcgcacca gtcgctgggc gatttcgggc agtgcgggca ggatctgccc
     8881 gctgaatttg tgaaaaccac ggtactggat aacacgccga tccgctggtt ttaccgggcg
     8941 gccagcctgg agtccgcgca gtgggcggcg gggcagaccg gggaaatcag ggtggacgtg
     9001 gagcgccgcc gtgccagccg ggaggcgggg aacgtggagc atatcagcgg ggacagcttc
     9061 atccagaagg aggcgcggcc gctgtttgac gtgaacaccc tgcaacacct gccggacggg
     9121 ttcgcggtca tgaccgggct gggtgtggcc cggatggcgt tcagcagtcc gctgcgggtt
     9181 gagcgccggg aaatcccgtt gcagcgtttc ccggtgctgg cgaaaaccga tcccctggcg
     9241 gagttccggc ccccggccga cggcggcggg ccgaaagcgg atgatgattt tgcg
//
