LOCUS       NZ_CP095131             2255 bp    DNA     circular CON 03-JUN-2024
DEFINITION  Escherichia coli strain Iso00270 plasmid pIso00270_06, complete
            sequence.
ACCESSION   NZ_CP095131
VERSION     NZ_CP095131.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17766603
            Assembly: GCF_022964775.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Pankok,F., Taudien,S., Dekker,D., Thye,T., Oppong,K., Akenten,C.W.,
            Lamshoeft,M., Jaeger,A., Kaase,M., Scheithauer,S., Tanida,K.,
            Frickmann,H., May,J. and Loderstaedt,U.
  TITLE     Microepidemiology of plasmids in Escherichia coli and Klebsiella
            pneumoniae with acquired extended spectrum beta-lactamase genes
            isolated from chronic wounds in Ghana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Pankok,F. and Taudien,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2022) Insitute of hospital hygiene and infection
            control, University Medical Center Goettingen (UMG), Humboldtallee
            34A, Goettingen, Lower Saxony 37073, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 16-FEB-2022
            Assembly Method                   :: Unicycler v. v0.4.8
            Assembly Name                     :: BNI_Ecoli_Iso00270_hybrid
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022964775.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 03:39:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,705
            Genes (coding)                    :: 4,489
            CDSs (with protein)               :: 4,489
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 68 of 216
            Pseudo Genes (incomplete)         :: 150 of 216
            Pseudo Genes (internal stop)      :: 44 of 216
            Pseudo Genes (multiple problems)  :: 42 of 216
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095131.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Iso00270"
                     /isolation_source="Chronic wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pIso00270_06"
                     /geo_loc_name="Ghana: Asante Akyem District"
                     /collection_date="2016"
     gene            complement(81..584)
                     /gene="ddp1"
                     /locus_tag="MU416_RS24025"
     CDS             complement(81..584)
                     /gene="ddp1"
                     /locus_tag="MU416_RS24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 1"
                     /protein_id="WP_000809479.1"
                     /translation="MKQIKFRLTDEEYNVAHINAISNGVNSVNAFAKRKVLDISATPSK
                     VIVSNEPSVPVKTCLYPHELELIKRNAALHGMSVSREIAIRLRKSVLKSEVCLYPEEVQ
                     VLRKLSTTINRIGRNIHFIISGDRFCTVNDPDFRNEIRELTALCETVDKTIEQLVFSVN
                     NRFG"
                     /name="ddp1"
     gene            complement(687..971)
                     /locus_tag="MU416_RS24030"
     CDS             complement(687..971)
                     /locus_tag="MU416_RS24030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000160398.1"
                     /translation="MTKHDAIRISQLHQIFYDDEGLIDSEKNVTILYSIGFTIDEIAYF
                     GNTTPGTVQGQLFNARAKLSCASASSLRPMILLRLLSLIIFEQDRLNRL"
                     /name="hypothetical protein"
     gene            complement(1046..1561)
                     /locus_tag="MU416_RS24035"
     CDS             complement(1046..1561)
                     /locus_tag="MU416_RS24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097059.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000097059.1"
                     /translation="MSSLSLQRYETNPFLELMTIPMKGKRIQVSRLGRDDNVLVNQNTG
                     EKFGTHVTTFKRVDSEQFVKLFTANIGLTFDLSSAGIKAFNVLLWAVQKQAFAKDQVLL
                     DSRALDDFLKSYDSLKLSYATLKRGINELEKAQIIAKTMRKGFYFINPNFVFNGDRIAF
                     TTLIERDC"
                     /name="replication/maintenance protein RepL"
     Typing          1831..2082
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.466"
                     /coverage="97.0"
                     /evalue="3.95e-106"
ORIGIN
        1 caaacgccgc aaaatgacgt ctctgacgca attctgcaac gtttgcccat gacctacagt
       61 cggttcttga taatacgttt ttacccaaaa cgattattaa cactaaaaac taattgctct
      121 attgttttat cgactgtttc gcataatgct gtaagttccc ttatctcatt tctgaaatca
      181 ggatcgttaa ctgtgcaaaa acgatcgcca ctaattataa aatgaatatt tcgaccaatt
      241 ctattaatag ttgttgaaag ctttctgagc acctgcactt cttcgggata aagacaaact
      301 tcgcttttca acactgattt acgtaagcgt attgctattt ctcggctaac gctcatcccg
      361 tgtaatgcag cattacgctt tattaactcc agttcatgtg ggtataaaca tgtttttact
      421 ggtacagatg gttcattcga tacgataacc tttgaaggtg ttgcgctaat atctaaaacc
      481 ttcctctttg caaaagcatt tacactatta acgccatttg aaatagcatt aatatgagca
      541 acgttgtatt cctcgtcggt caatctgaat ttaatttgct tcatagaacc tcctaagagc
      601 ctgcggcagg cacaggcaaa aaccccgttt ttggacaatc atggaaaatg gtacccattt
      661 gaccatgatt gtctatcctg cattgattac agcctattta agcggtcttg ctcaaagatt
      721 atgagtgaaa gcaatctcaa caaaatcatc ggtcttaaag atgaagctga agcacaactt
      781 aatttagctc ttgcattaaa taactgtccc tgaactgttc ccggtgttgt attcccaaaa
      841 taagctattt catcaatagt aaatcctatg ctgtataaaa tggtcacgtt cttttctgaa
      901 tcaattaatc cctcgtcatc gtaaaagatt tgatgaagtt gagaaatgcg tatagcatca
      961 tgcttagtca taataacctc cctaataggt tgttgtgatt agcagtcgaa caaaagtgga
     1021 gttttgttcg actgcgacga ttatattaac aatccctctc gatcagagtt gtaaacgcta
     1081 ttctatcgcc attaaataca aagttaggat ttatgaaata aaatcctttt cgcattgtct
     1141 ttgctataat ctgagctttt tctaactcgt tgatccctcg ctttaacgta gcgtaactta
     1201 gttttaagct atcatagctt ttcaaaaagt catcaagagc tctggaatct aaaagaacct
     1261 gatcttttgc aaaagcctgt ttttgaacag cccataacag gacattaaaa gctttaattc
     1321 ctgccgatga taaatcaaat gttagcccaa tattcgcagt aaacaattta acaaattgct
     1381 cactatccac acgcttaaaa gtggttacat gcgtcccaaa cttttctccc gtgttttgat
     1441 taaccaaaac gttgtcatca cgtccaagac gagatacttg tatccgtttc cccttcatcg
     1501 ggattgtcat aagctctaaa aatgggttcg tttcgtatct ctgcaagctt agtgagctca
     1561 ttattgcatc cttttttggg ctttggagat tcacaataaa tcctcataag ctcaaaatca
     1621 atgatttttt gagcctgcaa ggtaccggat ctgagcttac aaggtacaga tcctgatcac
     1681 cactttctag gattcatgcg ggtttcaaga aattcccttc ttagttctta actacgagcg
     1741 taagtgctca atatggcttc tggttctctt ccgcttcctc gcccactcgc tcactacgtt
     1801 cggtcgctgg ggctgcggcg agcggtgtca cctctcgcat gttgtcggtg atcccgttgt
     1861 tattctttcg gggatgagtc gtcagatagc ccctgaaacg cgctcagaag ctctcagaca
     1921 cacgagacgg ggtttaaatg cccgtcaggg catggaaggc gcgaaaaacg cttacagagg
     1981 gtttcaggag gtcaggcgcg ccgatacccc taaccagggg ctttcaggtc gccaggaacc
     2041 gcgtcagccc cctgcaaagc ccatttgcac ggaacaggca gccacgcggt tggtaggtgg
     2101 tcgtagaccg tcggtgttgc cgcgatgggg gtctggggtt ctccccagtc ggtttccggt
     2161 tgcgctcaag gcgcaatcgg aaccgtggcg gcttcggtcg ttccgaagaa cggggaataa
     2221 aaatcttcgt ggtttaaccc cgatgcagga taggg
//
