LOCUS       NZ_CP095130             3007 bp    DNA     circular CON 03-JUN-2024
DEFINITION  Escherichia coli strain Iso00270 plasmid pIso00270_05, complete
            sequence.
ACCESSION   NZ_CP095130
VERSION     NZ_CP095130.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17766603
            Assembly: GCF_022964775.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Pankok,F., Taudien,S., Dekker,D., Thye,T., Oppong,K., Akenten,C.W.,
            Lamshoeft,M., Jaeger,A., Kaase,M., Scheithauer,S., Tanida,K.,
            Frickmann,H., May,J. and Loderstaedt,U.
  TITLE     Microepidemiology of plasmids in Escherichia coli and Klebsiella
            pneumoniae with acquired extended spectrum beta-lactamase genes
            isolated from chronic wounds in Ghana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Pankok,F. and Taudien,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2022) Insitute of hospital hygiene and infection
            control, University Medical Center Goettingen (UMG), Humboldtallee
            34A, Goettingen, Lower Saxony 37073, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 16-FEB-2022
            Assembly Method                   :: Unicycler v. v0.4.8
            Assembly Name                     :: BNI_Ecoli_Iso00270_hybrid
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022964775.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 03:39:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,705
            Genes (coding)                    :: 4,489
            CDSs (with protein)               :: 4,489
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 68 of 216
            Pseudo Genes (incomplete)         :: 150 of 216
            Pseudo Genes (internal stop)      :: 44 of 216
            Pseudo Genes (multiple problems)  :: 42 of 216
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095130.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Iso00270"
                     /isolation_source="Chronic wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pIso00270_05"
                     /geo_loc_name="Ghana: Asante Akyem District"
                     /collection_date="2016"
     gene            complement(66..212)
                     /locus_tag="MU416_RS24000"
     CDS             complement(66..212)
                     /locus_tag="MU416_RS24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_077251022.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKAPLAAAIGKRVYKILLT
                     QSS"
                     /name="replication initiation protein"
     gene            complement(683..877)
                     /locus_tag="MU416_RS24005"
     CDS             complement(683..877)
                     /locus_tag="MU416_RS24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041619.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAENRTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1162..1485
                     /gene="mobA"
                     /locus_tag="MU416_RS24010"
     CDS             1162..1485
                     /gene="mobA"
                     /locus_tag="MU416_RS24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            2072..2404
                     /locus_tag="MU416_RS24015"
     CDS             2072..2404
                     /locus_tag="MU416_RS24015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013857758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_072842283.1"
                     /translation="MSNDDTEYTFKPVNAQALKALRSLPEWVQVAFVHALDSVFYGVDP
                     GMPIVHLTSIGPGVVELKINGRPAYRCVYRSKAPGQVDLLHAFVKTCQGPDKKNMATAT
                     SRYKLL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2421..2636)
                     /locus_tag="MU416_RS24020"
     CDS             complement(2421..2636)
                     /locus_tag="MU416_RS24020"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_193754969.1"
                     /translation="MIDNNWSQSEAAEKLGISQPRVSNMLNGKLDKFSIDTLLEMVFKM
                     GYKLDMDFNPLKTESPLTMVVKKAMV"
                     /name="helix-turn-helix transcriptional regulator"
     Typing          913..1153
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.76"
                     /coverage="100.0"
                     /evalue="2.14e-118"
     Typing          complement(141..648)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.629"
                     /coverage="95.0"
                     /evalue="4.94e-158"
     Typing          1163..1482
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="1.42e-62"
ORIGIN
        1 agggtgtaca agaccgctga aaccaaattc tgtcttttta gtttagctgt agtttggctc
       61 tgacttcaag aactctgcgt cagtaatatc ttgtacaccc tcttcccaat ggctgctgcc
      121 agtggcgctt tgtcgtgtct ttccgggttg gactcaagtt gatagttacc ggacaaggcg
      181 cagcggtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgcctac
      241 ccggaactga gtgtcaggcg tggaatgaga caaacgcggc cataacagcg gaatgacatt
      301 ggtaaaccga aaggcaggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
      361 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcggt ttttgtgatg
      421 ctcgtcaggg gggcggagcc tatggaaaaa cgcctgcggc gctggcttct tccggtgctt
      481 tgctgtttgc tcacatgttc tttccggctt tatcccctga ttctgtggat aaccgtatta
      541 ccgcttttga gtgagctgac accgctcgcc gcagtcgaac gaccgagcgt agcgagtcag
      601 tgagcgagga agcggaagag cgccttatgt gacattttct ccttacgctc ctctatgccg
      661 ttctgcatcc tgtccggatg cgttatatcc cggtgcgatt ttccgcttca aagcgtgtct
      721 gtatgctgtt ctgaagttct tccgcgagtt cgtgcagctt ctcacacatg gcggcctgtt
      781 cgtcagcatc gagtgcgtcc agtttttcga gcagcgtcag gctctggctt tttatgaatc
      841 cggccatgtt gagtacggct tgctgctgct tgttcatctt ttcgttttct ccgttctgtc
      901 tgtcatctgc gttgtgtgat tatatcgcgt accacttttc gactgtttta ccgccggtat
      961 tctgccgtct gactctttga cgggcatttc tgcctgacaa tactgtcact gccaaaaaac
     1021 tgccgtgcct ttgtcggtaa ttcgaacttg ctgacaggac aggatgtgca attgttatac
     1081 cgcgcataca tgcacgctat tacaattacc ctggtcaggg cttcgccccg acaccccatg
     1141 tcagatacgg agccatgttt tatgacaaaa cgaagtggaa gtaatacgcg caggcgggct
     1201 atcagtcgcc ctgttcgtct gacggcagaa gaagaccagg aaatcagaaa aagggctgct
     1261 gaatgcggca agaccgtttc cggtttttta cgggcggcag ctctcggtaa gaaagttaac
     1321 tcactgactg atgatcgggt actgaaagaa gttatgagac tgggggcgtt acagaaaaaa
     1381 ctctttatcg acggcaagcg tgtcggggac agggagtatg cggaggtgct gatcgctatt
     1441 acggagtatc accgtgccct gttatccagg cttatggcag attagcttcc cggagagaaa
     1501 actgtcgaaa acagacggta tgaacaccgt aagctcccaa agtgatcacc attcgctttc
     1561 atgcatagct atgcagcgag ctgaaaacga tcctgacgca tttttccggt tttcgcttgg
     1621 taacacatct ctttttgcgg tgtctgcggc agaatcccgt tcagcgcgtt tctgcggtgc
     1681 gtacaattaa gggattatgg taaatctatc attggcgagg tttcttcatt ggtttttatc
     1741 tagcaatcgg gcttctctgg ctacagttct gtttgcctaa ataaccctct ttagtgtcat
     1801 gacttaaggt aaaccgcttt ttttttgagc ggttagtttt gtataccttt gataaaactt
     1861 gattgcatat aagtatttgt atactgtgtc agtgttggcg agattgccaa taattattgg
     1921 ttctacgagc taatgaccat acgcgcactg acctaaaaaa tagggcggtg tggttttttt
     1981 atgctcacag atcaattaat cggagtattt atgcgaaata tcaatacggt agttaatttg
     2041 ttaaacatca ataacctaag agaggattgc tatgtcaaac gacgacactg aatatacgtt
     2101 caaacctgtt aatgcccagg ctttaaaggc attgagatca ctccctgagt gggttcaggt
     2161 tgcattcgtg cacgcattgg attcagtttt ctacggagtg gatccgggga tgcccatcgt
     2221 gcacttgacg tccatcggac ccggtgttgt tgagttgaaa atcaatggtc gccctgctta
     2281 tcgttgcgtt tatcgcagca aagctccagg tcaagtggac ttgctgcatg cgttcgtgaa
     2341 aacgtgtcaa ggacccgata agaagaatat ggcaaccgca acatcacgct acaagttgtt
     2401 gtaatccata aaggcgatgc ttacaccatc gcctttttta caaccattgt gagtggtgat
     2461 tctgttttta atgggttgaa atccatatct agcttgtaac ccatcttgaa caccatttct
     2521 aacaatgtgt cgatggagaa tttgtctagc ttcccgttca gcatgttact tactcggggc
     2581 tgactgatac ctaacttttc agctgcttca gattgagacc aattgttatc gatcattaac
     2641 tttctgatta ttattatgag ttttgatttt atcgaccatg tgttgaattt caccgggtcg
     2701 gtacttatca gctcaaatga atttttcata gaacagctcc atgttggatt acctgtacct
     2761 cgatgataaa agttccgaca aggaactcct tatatatata tatatatatg ggcggggcat
     2821 tatacaagta atgatatctc tttcaagaga aaaagatgtg aattgcattt gatcttgcaa
     2881 gtaacaatga tggtattgac gtgactctta agcaaagagt agtcttgttc aagaaacaaa
     2941 aaaaccgcct ggggaggcgg tttttttgag ggttcagtga gttggggaac ttctgaacca
     3001 tggtaac
//
