LOCUS       NZ_CP094996             4352 bp    DNA     circular CON 10-JUN-2024
DEFINITION  Klebsiella pneumoniae strain SCPM-O-B-8919 (12PKP/19c) plasmid
            pB-8919_5, complete sequence.
ACCESSION   NZ_CP094996
VERSION     NZ_CP094996.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18878040
            Assembly: GCF_018283575.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Fursova,N., Kuzina,E., Novikova,T., Kislichkina,A., Sizova,A.,
            Mukhina,T., Solomentsev,V. and Bogun,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2022) Science Department, SRCAMB, Building 1,
            Obolensk, Moscow Region 142279, Russia
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Fursova,N., Kuzina,E., Novikova,T., Kislichkina,A., Sizova,A.,
            Mukhina,T., Solomentsev,V. and Bogun,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2021) Science Department, SRCAMB, Building 1,
            Obolensk, Moscow Region 142279, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 58.16x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018283575.2-RS_2024_06_10
            Annotation Date                   :: 06/10/2024 05:04:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,920
            CDSs (total)                      :: 5,794
            Genes (coding)                    :: 5,644
            CDSs (with protein)               :: 5,644
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 60 of 150
            Pseudo Genes (incomplete)         :: 89 of 150
            Pseudo Genes (internal stop)      :: 22 of 150
            Pseudo Genes (multiple problems)  :: 18 of 150
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP094996.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SCPM-O-B-8919 (12PKP/19c)"
                     /isolation_source="intestine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pB-8919_5"
                     /geo_loc_name="Russia: Moscow"
                     /collection_date="2019-10-31"
     gene            join(3617..4352,1..131)
                     /locus_tag="KF986_RS29485"
                     /old_locus_tag="KF986_029485"
     CDS             join(3617..4352,1..131)
                     /locus_tag="KF986_RS29485"
                     /old_locus_tag="KF986_029485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_117045258.1"
                     /translation="MFKIENNKDILKSQIPLKPYASNDLDFGLTIQNKNKALDMLYLQV
                     NHPLYLHTMVFDIDKENCFYEFENAHLPIPSFITKSPDSGRCHYGYMLNAPISSTEKSR
                     QKPVKFARALYYNMATRLGADLGYAGLITKNPYSPHWSPFWSGADLYELNDLADCFDDL
                     EEPKKRENTGLAFGRNVEMFDTIRQWAYKNVLKYQSESSFNDFHNELLLKCQMHNAYIN
                     ADDLLPYNEIKATAKSIAKFCWKEFSEEKLNKIQSKKQKARRSKRTEKQKLHTKTLLEK
                     AIMINEE"
                     /name="replication initiation protein"
     gene            135..407
                     /locus_tag="KF986_RS29490"
                     /old_locus_tag="KF986_029490"
     CDS             135..407
                     /locus_tag="KF986_RS29490"
                     /old_locus_tag="KF986_029490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="WP_097346504.1"
                     /translation="MTALHTVKRNKTAKEMAQRLNISDRTVRKLIALPREDYEKEAKQR
                     RLKAFVLRESGMKWADVGIALGTTKHGAIALYKRYQQIDAPIKEA"
                     /name="HTH domain-containing protein"
     gene            complement(416..1885)
                     /locus_tag="KF986_RS29495"
                     /old_locus_tag="KF986_029495"
     CDS             complement(416..1885)
                     /locus_tag="KF986_RS29495"
                     /old_locus_tag="KF986_029495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_117045257.1"
                     /translation="MKGNVAKTGKSFKNRVDYILKDDHSFICSNMASDYNNVSDLTDEF
                     KNVSSFRPDIKKPVFHAFLSLPKDEKLTDEQWQEIAKDYLKEMNIDIEKHQYICVRHKD
                     TDKDHIHIVANRVGLDGSVWLGQHSAFNTIAACERLEVKHGLTITDGLKGQKSEVSAPT
                     KNEIEQALRKGEKPARIVLQNALQAAMLGKPDLQTFIDRVQAVGIEPRFNVASTGNVAG
                     CSFSVGDVAFKGSQLGKKYSWNTIKNKVIYDKNRDDELVRRFSARKADDENSIGRSFVE
                     SNINDSRAVDGIDDDIIRISQHSIKSDTTDTYEGKQLVRSNNHSFKLSEKYNRPTNKKI
                     GRATHKAAQILNRVKKQYNKEPIKTYNKSSFNRGSNTFSGSSISRILDHAETATTATNN
                     FRLKGRRSFNSRATKIEEIRGRLGIEKPVIQPRKIVKEEIKKKPLGLSMNENRFKSKLK
                     EALKKEKPKNETTPLQQEDDQHLKPRGFKPR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1882..2250)
                     /gene="mobC"
                     /locus_tag="KF986_RS29500"
                     /old_locus_tag="KF986_029500"
     CDS             complement(1882..2250)
                     /gene="mobC"
                     /locus_tag="KF986_RS29500"
                     /old_locus_tag="KF986_029500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_058685419.1"
                     /translation="MDTDRKRGRIGLNHEVRRDISIQFRVNKAEFDYLKELVEASGLER
                     GHYIRKEILNSEPVIKRKMPEFEQSLYREIQHQGNNINQIAKKLNTNEDFKQSVAAEAN
                     FAIKQLMKRILKACEVYV"
                     /name="mobC"
     gene            complement(2263..2520)
                     /locus_tag="KF986_RS29505"
                     /old_locus_tag="KF986_029505"
     CDS             complement(2263..2520)
                     /locus_tag="KF986_RS29505"
                     /old_locus_tag="KF986_029505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058685420.1"
                     /translation="MKIVLSVSKCLYINDLDRLLPCDRFFVHLDYKDISKKESFEMNRH
                     DQESYKLIFDKIVNNDVMLAIDEDIYSEVIENLLYYSNEK"
                     /name="hypothetical protein"
     gene            complement(3101..3316)
                     /locus_tag="KF986_RS29510"
                     /old_locus_tag="KF986_029510"
     CDS             complement(3101..3316)
                     /locus_tag="KF986_RS29510"
                     /old_locus_tag="KF986_029510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_117045259.1"
                     /translation="MQKPKRKLGFMVGKSNATLPPDDETEAELDQEVLKLFYPNQCTKD
                     TIERSEAGTDVFTASGSDDLFKKLKL"
                     /name="hypothetical protein"
ORIGIN
        1 aattcagcga agaaaaactc aacaagatac aaagtaaaaa acagaaagca agaagatcta
       61 aaagaacaga aaaacaaaag ctacatacaa aaacactttt agaaaaagca ataatgatta
      121 atgaggagta aaatatgacg gcattacata cagtaaaaag aaataaaaca gccaaagaaa
      181 tggctcaaag attaaatatc agtgatagaa ccgtcagaaa gttgattgct ttacctcgtg
      241 aagactatga gaaagaagca aaacaaagac gattaaaagc ctttgtatta cgtgaaagcg
      301 ggatgaaatg ggcagatgtt ggtattgctc ttggaactac aaaacacggt gctatagctc
      361 tttataaacg ttatcaacaa atagacgcac caataaaaga ggcttaaagc ctcttttatc
      421 ttggtttaaa acctcttggt tttaaatgct gatcgtcttc ctgctgtagt ggtgttgttt
      481 cattttttgg tttttctttt ttaagagctt cttttaactt cgatttaaat ctattttcat
      541 tcatactcaa acctaaaggt tttttcttta tttcttcttt aactattttc cttggttgaa
      601 taactggttt ttctatccca agtctacctc ttatttcttc gatcttagtt gctctactat
      661 tgaaactgcg acggcctttg agtctaaagt tattcgtggc tgttgtggct gtttctgcat
      721 gatcaagtat cctactgata gaactgccgc taaatgtatt gctcccacga ttaaagctac
      781 ttttattata agttttgatt ggttctttat tgtattgttt tttaactctg ttgagtattt
      841 gtgcagcttt atgtgttgct cttccaatct ttttgtttgt tggtctatta tatttttcgc
      901 tgagtttaaa gctgtggttg ttgcttctaa cgagttgttt accctcgtaa gtgtctgttg
      961 tgtctgattt aatgctgtgc tgactgattc ggataatatc atcatcgatt ccgtcaacgg
     1021 ctctactgtc attgatattt gattcaacaa atgatcgtcc aatactattt tcgtcatctg
     1081 cttttcttgc tgagaatctt ctaacgagtt catcatctct atttttgtca tatatcacct
     1141 tgttttttat agtgttccaa ctgtattttt tacctaactg cgaacctttg aaggccacat
     1201 ctcccacact gaaagaacaa cccgccacgt tgccagtgct ggcaacattg aaccttggtt
     1261 caatgcctac ggcctgcact ctgtcaataa acgtctgcag atctggttta cccaacatag
     1321 ccgcctgtaa tgcgttctgt agaacgatac gagctggttt ctcacctttg cgtagggctt
     1381 gctcgatctc gttcttggtt ggtgctgata cctctgattt ttgacctttt aagccatccg
     1441 tgatggttaa gccgtgtttt acttctaaac gttcacaagc tgctattgtg ttaaatgctg
     1501 agtgctgacc aagccatacc gaaccatcca gcccaaccct atttgctaca atatgaatat
     1561 ggtctttatc tgtatcttta tgacgcacac aaatatattg atgtttttct atatcgatat
     1621 tcatttcctt caaatagtct tttgctattt cttgccattg ttcatctgtg agtttctcat
     1681 cttttggaag agataaaaaa gcatgaaaaa caggtttttt tatatcaggt ctaaagctgc
     1741 ttacgttttt aaactcatca gttaaatcac taacattatt ataatcactt gccatattgc
     1801 tacaaataaa actgtgatca tcctttaata tataatcaac tctattttta aaagacttgc
     1861 ccgtcttggc tacatttcct ttcatacata aacctcacaa gcttttaata ttcttttcat
     1921 aagttgcttt atagcaaagt tagcttctgc ggctacagat tgtttaaagt cttcattcgt
     1981 atttagcttt ttagcaatct gatttatatt attaccttga tgttgtattt ctctgtataa
     2041 tgattgttca aactcaggca tttttctttt tataacgggt tcagagttta aaatctcttt
     2101 tcggatgtaa tgacctcttt caagccctga tgcttccaca agctctttta aataatcaaa
     2161 ctctgctttg tttactctaa actgtataga tatatctcta cgtacttcgt gatttaatcc
     2221 tatacgccct ctttttctat ctgtatccat atatataact ccttatttct catttgaata
     2281 ataaagtaag ttttcgataa cttcgctgta gatgtcttca tcaatagcaa gcattacgtc
     2341 attatttaca atcttatcaa agataagttt gtaactttct tggtcgtgtc tattcatctc
     2401 aaatgattcc tttttgctaa tatccttata atctaaatga acaaaaaaac gatcacacgg
     2461 aagtaagcga tctaaatcgt ttatgtataa acattttgat actgataaaa ctattttcat
     2521 atttattcct ttttgttttg aagtgaactg taaaactatt taccgcttcc ccttgatagg
     2581 ggaataagcg gaagggtaga tcttttaaca accctccgaa cgaagtgaga gggggcaacc
     2641 tgaaaggttg ggtataatac cggctacggg attacacctt gccttagttt ccgaaaaaca
     2701 aaaataaagc tgaactcaac ctatatttaa ataaataacc gatctaaaaa gtttgtcaat
     2761 atagcggcta cataaataaa taccctgcac aacaaataaa atgcaaataa aaaatagggt
     2821 aaataatata ttgtcttgat gggtagctgt gcgtaatagg tagctttgcg taaaaaatgc
     2881 cgctgctacg tgaaatctgg aaaagttcgt gaccatcaga tcaccagatt ttaaggaatg
     2941 gggtttgatc ggcggcgaca gaaacgggca aaacttgctt ttgtgcgttt gctggcgtct
     3001 atgggtttaa aatggatata ctgcatcgag ggttggcctt ttcatcgtgt ccggggtttg
     3061 gtttgttcca actggtgaag atggccatct ctcccctgcc ctacagcttc agttttttga
     3121 acaggtcatc agaaccggag gccgtgaaaa cgtctgtacc agcttctgag cgttctatgg
     3181 tgtctttggt gcattggtta ggataaaaca gcttcaggac ttcctgatca agctctgctt
     3241 ctgtttcgtc atctggtggc aatgttgcat tgctcttacc aaccatgaag cccagcttac
     3301 gctttggttt ctgcatctca tgctctccta tccagcacag ccagatctta catagagttt
     3361 agtatacgtt acagtgtgac ttactctgta gatggccttc ggcctgtgct tcgctgttgt
     3421 gtagttttcc aaaaactccg tctaatacct gatattggta gacttcctac ttaacaactt
     3481 tttttgccgg atctattgac aagttttttt ggtatagtaa aactgtaaga gattataaat
     3541 aaaagaaaag cccgcgttct ctgattagtt tggcgaccag tgagagaacg gggcattaca
     3601 gaaaggatac tgctatatgt tcaagataga aaataacaaa gatatattaa aaagtcaaat
     3661 acctttaaag ccgtatgcat caaatgatct tgattttggt ttgactattc aaaataagaa
     3721 taaagcatta gatatgcttt atttacaagt aaatcacccg ctttatcttc atacaatggt
     3781 atttgatata gacaaagaaa actgctttta cgagtttgaa aatgcccatt tgcctatacc
     3841 gtcttttata acaaaaagcc ctgatagtgg acgttgccat tatggttata tgcttaatgc
     3901 tcctatatct tctacagaga aaagcagaca gaaacctgta aagtttgcca gagcattata
     3961 ttataatatg gctactcgtt taggtgctga tcttggttat gctggactga ttactaaaaa
     4021 tccatacagt ccgcattggt cgccgttctg gtcaggtgct gatttatacg aacttaatga
     4081 tttagctgat tgttttgatg atttagaaga accaaaaaaa cgagaaaata cggggcttgc
     4141 ttttgggcgt aatgttgaaa tgttcgacac cataaggcaa tgggcttata aaaatgtgct
     4201 aaaatatcag agtgaaagct catttaatga ttttcacaat gaactattgc taaagtgtca
     4261 aatgcataat gcatatataa atgctgatga tttactgcca tataatgaga taaaagccac
     4321 agcaaaaagt atagcaaagt tctgttggaa ag
//
