LOCUS       NZ_CP094995             9294 bp    DNA     circular CON 10-JUN-2024
DEFINITION  Klebsiella pneumoniae strain SCPM-O-B-8919 (12PKP/19c) plasmid
            pB-8919_ColRNAI, complete sequence.
ACCESSION   NZ_CP094995
VERSION     NZ_CP094995.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18878040
            Assembly: GCF_018283575.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Fursova,N., Kuzina,E., Novikova,T., Kislichkina,A., Sizova,A.,
            Mukhina,T., Solomentsev,V. and Bogun,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2022) Science Department, SRCAMB, Building 1,
            Obolensk, Moscow Region 142279, Russia
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Fursova,N., Kuzina,E., Novikova,T., Kislichkina,A., Sizova,A.,
            Mukhina,T., Solomentsev,V. and Bogun,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2021) Science Department, SRCAMB, Building 1,
            Obolensk, Moscow Region 142279, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 58.16x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018283575.2-RS_2024_06_10
            Annotation Date                   :: 06/10/2024 05:04:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,920
            CDSs (total)                      :: 5,794
            Genes (coding)                    :: 5,644
            CDSs (with protein)               :: 5,644
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 60 of 150
            Pseudo Genes (incomplete)         :: 89 of 150
            Pseudo Genes (internal stop)      :: 22 of 150
            Pseudo Genes (multiple problems)  :: 18 of 150
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP094995.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SCPM-O-B-8919 (12PKP/19c)"
                     /isolation_source="intestine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pB-8919_ColRNAI"
                     /geo_loc_name="Russia: Moscow"
                     /collection_date="2019-10-31"
     gene            complement(128..547)
                     /locus_tag="KF986_RS29430"
                     /old_locus_tag="KF986_029430"
     CDS             complement(128..547)
                     /locus_tag="KF986_RS29430"
                     /old_locus_tag="KF986_029430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            950..2635
                     /locus_tag="KF986_RS29435"
                     /old_locus_tag="KF986_029435"
     CDS             950..2635
                     /locus_tag="KF986_RS29435"
                     /old_locus_tag="KF986_029435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            2645..2902
                     /locus_tag="KF986_RS29440"
                     /old_locus_tag="KF986_029440"
     CDS             2645..2902
                     /locus_tag="KF986_RS29440"
                     /old_locus_tag="KF986_029440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            2987..3136
                     /locus_tag="KF986_RS29445"
                     /old_locus_tag="KF986_029445"
     CDS             2987..3136
                     /locus_tag="KF986_RS29445"
                     /old_locus_tag="KF986_029445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            4617..6581
                     /locus_tag="KF986_RS29450"
                     /old_locus_tag="KF986_029450"
     CDS             4617..6581
                     /locus_tag="KF986_RS29450"
                     /old_locus_tag="KF986_029450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            6581..7312
                     /locus_tag="KF986_RS29455"
                     /old_locus_tag="KF986_029455"
     CDS             6581..7312
                     /locus_tag="KF986_RS29455"
                     /old_locus_tag="KF986_029455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            7319..7849
                     /locus_tag="KF986_RS29460"
                     /old_locus_tag="KF986_029460"
     CDS             7319..7849
                     /locus_tag="KF986_RS29460"
                     /old_locus_tag="KF986_029460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7877..8056)
                     /locus_tag="KF986_RS29465"
                     /old_locus_tag="KF986_029465"
     CDS             complement(7877..8056)
                     /locus_tag="KF986_RS29465"
                     /old_locus_tag="KF986_029465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8125..8502
                     /locus_tag="KF986_RS29470"
                     /old_locus_tag="KF986_029470"
     CDS             8125..8502
                     /locus_tag="KF986_RS29470"
                     /old_locus_tag="KF986_029470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(8564..8863)
                     /locus_tag="KF986_RS29475"
                     /old_locus_tag="KF986_029475"
     CDS             complement(8564..8863)
                     /locus_tag="KF986_RS29475"
                     /old_locus_tag="KF986_029475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8853..9116)
                     /locus_tag="KF986_RS29480"
                     /old_locus_tag="KF986_029480"
     CDS             complement(8853..9116)
                     /locus_tag="KF986_RS29480"
                     /old_locus_tag="KF986_029480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     BGC             1..7635
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(3240..3818)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6582..7309
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 taaaatttat cgggcgcgtt tccggcagtt ttccgggggg gttgttgcct gttttatccc
       61 gtagccgccg gaaacgccct gagcccgtct gagcggtgcg cgtaatgacg cgttatggta
      121 aatccactta ctgtctgcct tcatagccat cgagataaac agctgaaatg gtgtcagcca
      181 gtagccttga ttttttcggt gtgaggtctt tgctgcggta atcggtaccc gcaaattgtg
      241 ttgtgccttg gagtgagttt aaaaattcct gactgtgaaa ctgctgaatg cgttgctgca
      301 catcactccg tgattttccg gtcgctttat cctttgcgcc catatcccag aactgccgga
      361 aaaaaggcac catcattcgt gttgtgtcag ctttctgggt ggaaaaattg ggatcaaaac
      421 cgtcatctga agcatatacg taatgccggg tatgctgttc cacgctgccc tttggagcac
      481 aggcagtgag agttgctgtc gctgttatca tggcgataag acacagtttg ctgccggaac
      541 gtctcatgag cgtctcctta cctttaattc ttttttctca ttgagttata tatccgcagg
      601 ctatcttatc atcagtttct gaccgtgttc agtgaccgaa caacccgaac cagacccccc
      661 gtttcttttc ctgtcccgga gggggctttc tggtatctca tataatgatt tcagtaaaac
      721 atactgaggc cggagaacgg cgccgtttgt gctggttttt gttgcatctg gaggggattg
      781 cgcatggtgt gagtggctgt ttttttgatc attttcacaa aaacgcaaat ttacagactt
      841 gacatgaaaa acaaggcagc gtaattttta tactgtatat aaaaccagtg gttatgtgta
      901 cagtatttta ttttagttta ttgttttaaa agtcaaagag gaaaagtata tgagtggcgg
      961 agacggacga ggtccgggta attcaggtct gggacacaat ggtggtcagg ccagtgggaa
     1021 tgtgaacggt acatcaggta aaggtggccc ttcatcaggt ggtggtacgg atccaaacag
     1081 cgggccgggc tggggtacga cgcatacacc taacggggat attcataact ataacccggg
     1141 ggagtttggt aacggcggga gtaaacccgg tggtaatggc ggtaacagcg gcaatcatag
     1201 cgggagctct ggtggcggac agtcttcggc caccgcgatg gccttcggtc tgccggcctt
     1261 ggccactccg ggggctgaag gactggcttt atccgtttcc ggcgatgcgt tgtcggccgc
     1321 tgttgctgat gtgctggctg ccctgaaagg tccgtttaag tttggtctgt gggggattgc
     1381 gatttacgga gtgctgcctt ctgagatagc aaaagatgat ccgaaaatga tgtcaaaaat
     1441 cgtgacgtca ttgccggcag acacggtaac ggagacgccg gtcagctccc tgccgctgga
     1501 ccaggcgacg gtcagcgtca ctaaacgtgt ggcggatatt gtgaaggacg agcggcagca
     1561 tattgcggtt gtcaccggcc ggccaatgag tgttcctgtg gtggatgcga aaccgacaaa
     1621 acgtccgggg gtattcagtg tgtcgattcc gggtctcccg tctctgcagg tgagcgtacc
     1681 taaaggtgtt ccggccgcga aagccccgcc aaaaggcatt attgctgaaa aaggtgattc
     1741 acgtccggct ggttttacgg ccggtggtaa ctcccgtgaa gccgttattc gtttcccgaa
     1801 agagagcgga cagaaaccgg tctatgtgtc ggtgacggat gttcttactc cggcacaggt
     1861 aaaacagcgt ctggaggaag aaaagcgtcg ccagcaggca tgggacgctg ctcacccgga
     1921 agaggggctg aaaagagagt atgataaagc gaaagctgag ctggatgctg aagataaaaa
     1981 tattgcgacc ttaaacagtc gcattgcatc gacagagaag gcgatccccg gtgcaagggc
     2041 tgctgttcag gaagccgata aaaaggtaaa agaggcagag gcaaataaag atgattttgt
     2101 gacttataac ccgcctcatg aatatggctc cgggtggcag gatcaggttc gctatcttga
     2161 taaggatatt cagaatcaga atgagaaatt aaaagctgcc cagacatctt taaacgaaat
     2221 gaatgaatcc ttatccaggg ataaggctgc gctttccggg gcgatggaga gccggaaaca
     2281 aaaggagaaa aaagcgaagg atgcagaaaa taaattaaat gaggaaaaga aaaaacctcg
     2341 taagggaact aaagattacg gccatgatta ttttcctgat cccaagactg aagatattaa
     2401 ggggttggga gagttgaaag agggtaaacc taaaacccct aaacaaggtg gtggtggtaa
     2461 gcgggcccga tggtatggtg ataaaaagcg taaaatttat gaatgggatt cccagcacgg
     2521 tgagcttgaa gggtaccgcg ccagtgatgg cgaacacctc ggggcattcg atccaaaaac
     2581 gggtaagcag gttaaagggc cggatccaaa acgaaacatt aaaaaatatc tttaagaggt
     2641 aaatatgggg cttaaattac atattcattg gtttgataag aaaaccgaag agtttaaagg
     2701 tggtgaatac tcaaaagact tcggtgatga tggttctgtc attgaaagtc tggggatgcc
     2761 tttaaaggat aatattaata atggttggtt tgatgttgaa aaatcatggg tttcgatatt
     2821 acagccacac tttaaaaatg taatcgatat tagtaaattt gattactttg tatcctttgt
     2881 ttaccgggat ggtaactggt aagcaaaagt tatcaaggat gatttgaaat acagacttcc
     2941 ggtgttcagg gatgaatacc ggggttttta aatacaggga aggtatatga aaaaggtaaa
     3001 ggttgttttt ctttttatat tgattttttc aggctttctt ctggtcgcat gtcaggcaaa
     3061 ctatatccgg gatgttcagg gtggaacggt ggcaccatcg tcctcctctg aactgacggg
     3121 gatcgcggtt cagtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3181 atcttttgcc ctgtaaacga aaaaaccacc tggggaggtg gtttgatcga aggttaagtc
     3241 agttggggaa ctgcttaacc gtggtaactg gctttcgcag agcacagcaa ccaaatctgt
     3301 ccttccagtg tagccggact ttggcgcaca cttcaagagc aaccgcgtgt ttagctaaac
     3361 aaatcctctg cgaactccca gttaccaatg gctgctgcca gtggcgtttt gcgtgctttt
     3421 ccgggttgga ctcaagtgaa cagttaccgg ataaggcgca gcagtcgggc tgaacggggg
     3481 gttcttgctt acagcccagc ttggagcgaa cgacctacac cgagccgaga taccagagtg
     3541 tgagctatga gaaagcgcca cacttcccgc aagggagaaa ggcggaacag gtatccggga
     3601 aacggcaggg ttggaacagg agagcgcaag agggagcgat tcatcggaaa cggtgatgat
     3661 ctttaagtcc tgtcgggttt cgccactcct gactgattca tggttgagcc accggctccc
     3721 acagatgcac cgaaaaagcg tctgtttatg tgaactcagt caggagggcg gagcctatgg
     3781 aaaaacgcca cctgcgtggc cttgccgttt tgcctcacat gttagtcccc tgcttattca
     3841 cggaatctgt gggtaacttt gcatgtgtcc gcagcgcccg ccgcagtctc acggccggag
     3901 cgtagcgacc gagtgagcga ggaggcgcaa tttccccggc acctacaaga cggacaccgg
     3961 acgcgctgat gtggcatatt gtcccctgat taagtcttta tattccctga taataaatta
     4021 ccaggatagg tgaatcatat cggtttttcg ctgaacattt tacgttaacc tatctctgtt
     4081 gcttttttct ccttacggag aaatacaggt catccccgcg agggcgtggc tgacctcagg
     4141 gggatatccc ctgcttacgc tggctggacg ccaccgacgc acggtctctc cccctctttc
     4201 tggctccgtc cggctggacg ccggccacgc cagaaattca cccggccccc gtcaggctca
     4261 cgcctgacca cccacaaaaa tccacggtgc tggacgcacc tggcgaagca gcaaagctgc
     4321 tcctgatttt gcgggtggtc agccgctcgc tatggcgaac tctgacgggg gccggaacac
     4381 cggaaaagac cgcactgggg tgccgccttt tcctctcaca cgctggggcg tgctcgttgt
     4441 tcctccgggg ctgcgccttt tccgcatggg gcggaaagag gctggcctcc gtggtgaaag
     4501 ctgaacgcaa tcaccacgga cgtgaccgcc gccgccatgc cttggggcat gtccgcggct
     4561 tgtgcagcgg gggctgcaca tcacgccggc aaaccatcgt ctgaaggaga gagaacatgt
     4621 ttaatacgga ttcgcttgcc tggaagtggc acattccgga gctggcccgg cagatttatc
     4681 cgtgggtcag ctggctgtac caggagtatt acggtctggc cggggctttc ctagttatcg
     4741 gcctgttttt cggtctggct gaactttggg ccggacggat gcgttttgtg gggctgagtc
     4801 ataaggagcg acgggagtat gccgatcgct ataagcgcga taataaacgg ccagcgccat
     4861 tttcggtgct ggcctcgctg gattcgcccg ggcggatggt cagggcacgt cgacatgtct
     4921 ggaactacgc gtactggctg accggcgcgg ccggtctggc ccttctgggg gggctggttg
     4981 tcgccgtgtt tatcgagagc tggggagaaa ccgtcctgcg tccggacctc tacgcaaacg
     5041 cagattatta cctgctgcag ccggctaacc tgttcgggat tgcctggtgc gcgggcgggc
     5101 tggctgtcgg gattgtgttg gcgcggctgc tggcgttcct gctgcttgac ggtcattttg
     5161 cggctgctga cgaggcggtg aacgcgaaac tgaatcagga gagccggcgc agcagccagc
     5221 ggacaggaga gatgaccgac gtgcgccacc tgcatttcgg ggagccggta ccggttaatg
     5281 cgctggccga tttttcctcg gagcaggccc ggcaggagca ggcggtgttt ctgggtaagg
     5341 atgagcaggg caagccggtg ctggttccgc gtgatacctg gcgcaagacc aacattcaga
     5401 tcctcggcct gcccgggagc ggcaagagcg tgatggccac caacgcgctc attcgctgcg
     5461 tacgcgattt cggggacgcg gtggtgtact tcgaccccaa aggggacgca tgggcgccgc
     5521 acgtcttccg ggcgcactgt ccggacttca ccctgctgga tctgcggccg gggaaaccgg
     5581 cgcagctgaa tctgttccgc gatctcgacc agtacgcgct gaaaaacctg ctgattgccg
     5641 ggcttaacct gggcgagacc ggcgacgtgg cggaccacta ccgcatcagc gagcagaaag
     5701 cggcgaagct gattgcggag cagtttccgc agggggcaaa cattcagcag gtgctggcgg
     5761 cggcgtacgc gctgccggag gccctgaaaa aggacgtgaa ggggttgatc actaggctgg
     5821 aaaacgtcgc tgacctgagc gtcctgcaga cggacagtgg catcgatgtg gcggggatta
     5881 tcaacggcgg cggctgcctg tacgttatcg gctcgatgga cgacgaggcg gtgatccgtg
     5941 tgcaaaaaat gctgtttgcc cgctgcgcgc aaatcatcat tgcgcgggac gagttccggg
     6001 agtggccaca cgtcagcgtg atgctcgatg aaattaaata tctgctgtcg aagtatgtac
     6061 tgaacgcact ggggacgctg cgcagccggg actgcaatct gttgctcgcg caccagtcgc
     6121 tgggcgattt cgggcagtgc gggcaggatc tgcccgctga atttgtgaaa accacggtac
     6181 tggataacac gccgatccgc tggttttacc gggcggccag cctggagtcc gcgcagtggg
     6241 cggcggggca gaccggggaa atcagggtgg acgtggagcg ccgccgtgcc agccgggagg
     6301 cggggaacgt ggagcatatc agcggggaca gcttcatcca gaaggaggcg cggccgctgt
     6361 ttgacgtgaa caccctgcaa cacctgccgg acgggttcgc ggtcatgacc gggctgggtg
     6421 tggcccggat ggcgttcagc agtccgctgc gggttgagcg ccgggaaatc ccgttgcagc
     6481 gtttcccggt gctggcgaaa accgatcccc tggcggagtt ccggcccccg gccgacggcg
     6541 gcgggccgaa agcggatgat gattttgcgg ggatttactg atggcgctgg agctgtacaa
     6601 cgtcagccac gcgaaacgac aggcgcggaa cgcggagaaa acccggctga ccctgcgctg
     6661 gttgcgggaa gagctgtgtt ccacggcgga gctggttgcg cggcggctgg ggattgcggc
     6721 ggtgcagccg gtctaccgct tcctcgacag cctggtggcg aaagggctgc tggtccggac
     6781 caaatatccg gtggacggcc gtcaggtcag cgtgtggggg ctgacgccgc atggcgtggc
     6841 gttctcgttc gacgaggacg aaccgctggc ggatgttatt ccgtttcagc cgtcaagggt
     6901 gtcggcagca catctgccgc accggctggc cgtgcaatcc ctgcgccttg atatggaagc
     6961 cagaggggcc accggctggc gcattctgca ccgtctgagt cttaaaggca tgaaagtgcc
     7021 ggacgccctg gcggagctgg acggtaaaac ggtggcgttt gaggtggagc gcaccgtcaa
     7081 gagccgtcgc cggtatcagg aagtggtgtc cgggtatctg ttcaaccgca aagctaacgg
     7141 catcgatgaa atctggtaca tctgcccgga ccgggcgacg caggtcaggg tgcagcgggc
     7201 aatcctgtcg gtggatgaaa tcgtgaaccc acagaccggc gaggcccgca aaacggcaga
     7261 gctggatcgt gaacggttgt ttgcgtgctt taaatttatg acaacggagt gagggattat
     7321 ggcgctgtac atggtattta ttgaagacgc ggaggaccgg aacggcgagc gctgcatcat
     7381 gccgaccagc gagcatctgg aagatatggt ggtgctcgcc tggcagaccg gggcgtatgt
     7441 tttctgtttt gtcgaccggg cccagcatct ggcgatgatg aaatccatcg ggatgtatga
     7501 cgagcggctc aacagcgacg aaaaagccac gtactttttc actggtaaaa atccgcaatg
     7561 ggaacggaac aaacccacag gaatgaagct gtgtgattac ctctatcgca aggtggagcg
     7621 gttcgccatc gagaacggtg tgcaggtggt tgatgccggg aagtgctcac cggagcagat
     7681 taacgcgatg attgacgtgg tgagtgagcg gcacggggaa agcgcggtga acgagaagct
     7741 gaaagcggag ctgatgcggg aatataacag ccgctatttt aacgtgccgg tacctgaact
     7801 gccggaggac aaacctgccc gccggtggtg gccgttcggc aaaaaataaa tgccagcacc
     7861 ggccatgccg gtgctgttat tcctccccga gcttatcccg catcacctgc cagagtgttt
     7921 ccgcatcctc gtgtagccgc tcgcacatgt cggccggggt atccatatcc agctcattca
     7981 gtttatcgag caatagcagc gactgctcct taataaattt agccatattg agggctgttt
     8041 tttcctgttt tgtcatgatt ctttccttcc gggcatggtg tgcgcctgtc aggtatactg
     8101 tctcaaaaat agcggggtga gacaatggcg tgggtgacgg tgagacaggc gacggaactg
     8161 acgggtaaag cccgaagttc cctgtatcgg gatatggcga aaggccgcgt atcataccgt
     8221 acggaagctg atggcggccg ggtggtggat accagcgagc ttatccgggt gtacggtgag
     8281 ttaagacagg atgagactca cgagcgggac gagttgagac tgtcgggtga gactgaaaaa
     8341 atggcctccg acgcgttgat cgcggagata aaagcactca gggaggaggt ggccgggcta
     8401 cggcaggaaa tgcagtccat gagactgctg gaacacaaac cggagacagc agagaccccg
     8461 gccggaacac cgcgccggtg gtggccgtgg ggaaaacgct gacgcgctgt gcttgtccaa
     8521 ccggcgacgg ccacgcaatg aattgcgtcc ccgtcaccgg ttattagaga acaatatggg
     8581 gattctggcg ctggccgacc acggccagaa cccggacttt tctggtctgg tcatcaacat
     8641 catagaggac atgctgtccc atcagactga tccggcgaac gtcctcgcca tagcgtgagt
     8701 ccacttcata gagtcgcggg aagtcggcca gtttgtccgc cgcagcgaga acccggtcgg
     8761 taacgttctg cgcatcccac agggatgaat tttccgcgat atagtcaaac agcgtcgtta
     8821 catcctcgat ggctccctgc ttccattcca cgtcatacgg catcgcgcag ttcctgataa
     8881 cggccatgca ggcgctggcg gagttgttcc gtggtcatac ccggacgcgt gtcagcacgg
     8941 gcggcggtca ctttggcgcg aacctgatct tcatactctg cctgggccag ttcttcgtta
     9001 gccagtcgct gcaacagcgc agctttgtcc gggccacctt ccggcagtat gcggggcagg
     9061 ctttcagcca gttcccgaag aaagttggcg ttttcgtagt gtgtagtgtt gctcattgcg
     9121 gagcctcccg ggttaatgcg gcaatatgaa ggaagtttag cagggggcgg tctgaaagaa
     9181 tatggtcgtg tcagtcaggc tgatgaagca gaaagtcggt aaaaaagggc ttacggcatc
     9241 cattttacgt caaaacatat gccatgcagt tgcgtgcata ggcatgcatt agga
//
