LOCUS       NZ_CP094877             2189 bp    DNA     circular CON 20-APR-2024
DEFINITION  Staphylococcus epidermidis strain C101 plasmid pC101MK5, complete
            sequence.
ACCESSION   NZ_CP094877
VERSION     NZ_CP094877.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27161490
            Assembly: GCF_022832935.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Kopczyk,M., Mulroney,K., Chakera,A., Ramsay,J.P., Colombi,E.,
            Coombs,G.W. and Mowlaboccus,S.
  TITLE     Sequencing of Staphylococcus epidermidis isolated from peritoneal
            cavity
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Kopczyk,M., Mulroney,K., Chakera,A., Ramsay,J.P., Colombi,E.,
            Coombs,G.W. and Mowlaboccus,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2022) School of Pharmacy & Biomedical Sciences,
            Curtin University, Kent street, Bentley, WA 6102, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-MAR-2022
            Assembly Method :: Flye v. 2.9-b1774; Racon v. 1.4.15; Pilon v. 1.23
            Assembly Name                     :: C101MK-10-Mar-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 306.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022832935.1-RS_2024_04_19
            Annotation Date                   :: 04/19/2024 10:01:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,338
            Genes (coding)                    :: 2,264
            CDSs (with protein)               :: 2,264
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 46 of 74
            Pseudo Genes (incomplete)         :: 36 of 74
            Pseudo Genes (internal stop)      :: 27 of 74
            Pseudo Genes (multiple problems)  :: 26 of 74
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP094877.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="C101"
                     /isolation_source="pertoneal cavity"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="pC101MK5"
                     /geo_loc_name="Australia: Perth"
                     /lat_lon="31.92851574 S 115.86399442 E"
                     /collection_date="2017-04-28"
                     /collected_by="Harry Perkins Institute of Medical Research"
     gene            join(1389..2189,1..123)
                     /locus_tag="MUG62_RS12040"
                     /old_locus_tag="MUG62_12040"
     CDS             join(1389..2189,1..123)
                     /locus_tag="MUG62_RS12040"
                     /old_locus_tag="MUG62_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002499696.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_031864528.1"
                     /translation="MEKYTEKKRRNQVFQKFIERHVKEGQMDLIRECNTFLSFVADKTL
                     EKQKLHKSNLCKNRFCPVCAWRKARKDALGLSLMMQYIKQKEDKQFIFLTLTTPNVTAE
                     HLESEIKAYNHSFQKMFKRKKVISATKGYVRKLEITYNKERDDYNPHFHVLIAVNKSYF
                     TDKRYYISQKEWLELWRDVTGISEITQVQVQKIRQNNNKELYEMAKYSGKDSDYLINQK
                     VFDAFYKSLKGKQVLVYSGLFKDAKKKLKNGDLDYLKEIDPTEYVYQIFYMWNKNEYLA
                     SEIFDLTDEEKQKINHQMINEIEEEK"
                     /name="protein rep"
     Typing          1390..2185
                     /name="rep_cluster_1281"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.359"
                     /coverage="86.0"
                     /evalue="0.0"
ORIGIN
        1 tatcaaattt tttacatgtg gaataaaaat gaatatttag cgagtgaaat ttttgattta
       61 acagacgaag aaaaacaaaa aataaatcat caaatgataa atgaaattga agaagaaaaa
      121 taaaaaacaa aacgctactc gaaaaaaaag agtgagcgtt ttttgttttg ttagaaaaaa
      181 ttaaagatgt ttttctgaaa aaggtttgag attttgaaat gaaaaatatg agggtataca
      241 ggtgtaatat tgcaactatt gctattaaag agcataagag gaatcctaca cagaaatgtc
      301 tccgtatgta gccattcggt agccatttct gtgaaaaata ttgatttgac agtgtcaaat
      361 gggattccta tttatgcgat aaaaattgtt atatgactcc cgaacggcta cacaaaagat
      421 accgaatagc tacacaaaat caaaaatgga taccaaatag ctacattatt aggtagccat
      481 ttgggagcta aaagggagcc aaatggtagc caaaatcaaa atgcactgct gccttttttg
      541 aatattatta atcgggtttt agcttttttc taagaagaat aattgagatt aaaaatatat
      601 ttttctttct gaaagtgttg agaaatgaat tttaaaatga ttcatagcct ctcttttgaa
      661 cgaaagaaat ttttgtttgt ttttatattc gaaatgatta aatgtcctag aaagggctta
      721 aaatgcaaaa taaaagaacc actcaaaaat gagtggtttg gagaattgct ataaaatgct
      781 gtgcgattga aaaagagcac tatattaagc ataccacacg catttatgcc gagaaaattt
      841 attgtgcgtt gagaagaacc cttaactaaa cttgaagacg aatgtcggca tagcgtgagc
      901 tattaagccg acgattcgac aagttttggg attgttaagg gttcagaggc tcaacgtcaa
      961 taaagcaatt ggaataaagc ataaaaaata gccactcaac aatttgagta gctatggtga
     1021 gggcgaagag ttacttcgta ttacaaataa cttatacata gttgggataa cgttatatta
     1081 ttattttaac acttatttat ttttatctca tttaaaaact atttaacgac gggatttttt
     1141 gaatttttga aacgagtgaa acgagtttct tcttgtcttg atactatagg taactattcc
     1201 cgtatggtta cctgtctcta gattagttga tatgactgtc tttgaacgca aaaaaagttg
     1261 ctttttcata cctagtatag tagagtttat ggtgaccaaa ccaaaacttt acatgaaagg
     1321 agaaaaagca acttttttgt tataaaaact ttgtatacaa cttagttgtt tttacagtga
     1381 tttaatatat ggaaaagtat actgagaaaa aacggagaaa tcaagtattt cagaaattta
     1441 ttgaacggca tgtcaaagaa ggacaaatgg atttgataag ggagtgcaat acattcttga
     1501 gttttgtggc agataaaaca ttagagaaac agaaattgca taaatctaat ttatgtaaaa
     1561 atcgattttg tcctgtatgt gcatggcgaa aagcgagaaa agatgcgtta ggtttatcat
     1621 tgatgatgca atatattaag caaaaagaag ataaacaatt catcttttta acacttacca
     1681 cgccaaatgt tacagctgag catttagaaa gtgaaattaa agcttataat cactcttttc
     1741 aaaagatgtt taagcgtaaa aaagtaattt ctgctacgaa aggttatgta agaaaattag
     1801 aaattactta caataaagaa cgtgatgact ataacccgca ttttcatgtt ttaattgcag
     1861 taaataagtc atattttaca gataaaagat attatattag ccaaaaagaa tggcttgaat
     1921 tatggcgtga cgtaacggga atttcagaaa ttacacaagt tcaagttcaa aaaataagac
     1981 aaaataacaa taaagagtta tatgaaatgg ctaagtattc tggtaaagat agtgattatt
     2041 taataaatca aaaagtattt gatgcattct ataaatctct taaaggtaaa caggtattag
     2101 tttattcagg attatttaaa gatgctaaaa agaaattaaa aaatggagat ttagattact
     2161 taaaagaaat tgatccaacc gaatacgtg
//
