LOCUS       NZ_CP094864             2189 bp    DNA     circular CON 20-APR-2024
DEFINITION  Staphylococcus epidermidis strain C99 plasmid pC99MK5, complete
            sequence.
ACCESSION   NZ_CP094864
VERSION     NZ_CP094864.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27160588
            Assembly: GCF_022832895.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Kopczyk,M., Mulroney,K., Chakera,A., Ramsay,J.P., Colombi,E.,
            Coombs,G.W. and Mowlaboccus,S.
  TITLE     Sequencing of Staphylococcus epidermidis isolated from peritoneal
            cavity
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Kopczyk,M., Mulroney,K., Chakera,A., Ramsay,J.P., Colombi,E.,
            Coombs,G.W. and Mowlaboccus,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2022) School of Pharmacy & Biomedical Sciences,
            Curtin University, Kent street, Bentley, WA 6102, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-MAR-2022
            Assembly Method :: Flye v. 2.9-b1774; Racon v. 1.4.15; Pilon v. 1.23
            Assembly Name                     :: C99MK-9-Mar-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1552.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022832895.1-RS_2024_04_19
            Annotation Date                   :: 04/19/2024 10:06:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,425
            CDSs (total)                      :: 2,341
            Genes (coding)                    :: 2,266
            CDSs (with protein)               :: 2,266
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 48 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 27 of 75
            Pseudo Genes (multiple problems)  :: 27 of 75
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP094864.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="C99"
                     /isolation_source="pertoneal cavity"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="pC99MK5"
                     /geo_loc_name="Australia: Perth"
                     /lat_lon="31.92851574 S 115.86399442 E"
                     /collection_date="2017-04-14"
                     /collected_by="Harry Perkins Institute of Medical Research"
     gene            1..924
                     /locus_tag="MUB20_RS12045"
                     /old_locus_tag="MUB20_12045"
     CDS             1..924
                     /locus_tag="MUB20_RS12045"
                     /old_locus_tag="MUB20_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002499696.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_031864528.1"
                     /translation="MEKYTEKKRRNQVFQKFIERHVKEGQMDLIRECNTFLSFVADKTL
                     EKQKLHKSNLCKNRFCPVCAWRKARKDALGLSLMMQYIKQKEDKQFIFLTLTTPNVTAE
                     HLESEIKAYNHSFQKMFKRKKVISATKGYVRKLEITYNKERDDYNPHFHVLIAVNKSYF
                     TDKRYYISQKEWLELWRDVTGISEITQVQVQKIRQNNNKELYEMAKYSGKDSDYLINQK
                     VFDAFYKSLKGKQVLVYSGLFKDAKKKLKNGDLDYLKEIDPTEYVYQIFYMWNKNEYLA
                     SEIFDLTDEEKQKINHQMINEIEEEK"
                     /name="protein rep"
     Typing          2..920
                     /name="rep_cluster_1281"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.985"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atggaaaagt atactgagaa aaaacggaga aatcaagtat ttcagaaatt tattgaacgg
       61 catgtcaaag aaggacaaat ggatttgata agggagtgca atacattctt gagttttgtg
      121 gcagataaaa cattagagaa acagaaattg cataaatcta atttatgtaa aaatcgattt
      181 tgtcctgtat gtgcatggcg aaaagcgaga aaagatgcgt taggtttatc attgatgatg
      241 caatatatta agcaaaaaga agataaacaa ttcatctttt taacacttac cacgccaaat
      301 gttacagctg agcatttaga aagtgaaatt aaagcttata atcactcttt tcaaaagatg
      361 tttaagcgta aaaaagtaat ttctgctacg aaaggttatg taagaaaatt agaaattact
      421 tacaataaag aacgtgatga ctataacccg cattttcatg ttttaattgc agtaaataag
      481 tcatatttta cagataaaag atattatatt agccaaaaag aatggcttga attatggcgt
      541 gacgtaacgg gaatttcaga aattacacaa gttcaagttc aaaaaataag acaaaataac
      601 aataaagagt tatatgaaat ggctaagtat tctggtaaag atagtgatta tttaataaat
      661 caaaaagtat ttgatgcatt ctataaatct cttaaaggta aacaggtatt agtttattca
      721 ggattattta aagatgctaa aaagaaatta aaaaatggag atttagatta cttaaaagaa
      781 attgatccaa ccgaatacgt gtatcaaatt ttttacatgt ggaataaaaa tgaatattta
      841 gcgagtgaaa tttttgattt aacagacgaa gaaaaacaaa aaataaatca tcaaatgata
      901 aatgaaattg aagaagaaaa ataaaaaaca aaacgctact cgaaaaaaaa gagtgagcgt
      961 tttttgtttt gttagaaaaa attaaagatg tttttctgaa aaaggtttga gattttgaaa
     1021 tgaaaaatat gagggtatac aggtgtaata ttgcaactat tgctattaaa gagcataaga
     1081 ggaatcctac acagaaatgt ctccgtatgt agccattcgg tagccatttc tgtgaaaaat
     1141 attgatttga cagtgtcaaa tgggattcct atttatgcga taaaaattgt tatatgactc
     1201 ccgaacggct acacaaaaga taccgaatag ctacacaaaa tcaaaaatgg ataccaaata
     1261 gctacattat taggtagcca tttgggagct aaaagggagc caaatggtag ccaaaatcaa
     1321 aatgcactgc tgcctttttt gaatattatt aatcgggttt tagctttttt ctaagaagaa
     1381 taattgagat taaaaatata tttttctttc tgaaagtgtt gagaaatgaa ttttaaaatg
     1441 attcatagcc tctcttttga acgaaagaaa tttttgtttg tttttatatt cgaaatgatt
     1501 aaatgtccta gaaagggctt aaaatgcaaa ataaaagaac cactcaaaaa tgagtggttt
     1561 ggagaattgc tataaaatgc tgtgcgattg aaaaagagca ctatattaag cataccacac
     1621 gcatttatgc cgagaaaatt tattgtgcgt tgagaagaac ccttaactaa acttgaagac
     1681 gaatgtcggc atagcgtgag ctattaagcc gacgattcga caagttttgg gattgttaag
     1741 ggttcagagg ctcaacgtca ataaagcaat tggaataaag cataaaaaat agccactcaa
     1801 caatttgagt agctatggtg agggcgaaga gttacttcgt attacaaata acttatacat
     1861 agttgggata acgttatatt attattttaa cacttattta tttttatctc atttaaaaac
     1921 tatttaacga cgggattttt tgaatttttg aaacgagtga aacgagtttc ttcttgtctt
     1981 gatactatag gtaactattc ccgtatggtt acctgtctct agattagttg atatgactgt
     2041 ctttgaacgc aaaaaaagtt gctttttcat acctagtata gtagagttta tggtgaccaa
     2101 accaaaactt tacatgaaag gagaaaaagc aacttttttg ttataaaaac tttgtataca
     2161 acttagttgt ttttacagtg atttaatat
//
