LOCUS       NZ_CP094861             7305 bp    DNA     circular CON 20-APR-2024
DEFINITION  Staphylococcus epidermidis strain C99 plasmid pC99MK2, complete
            sequence.
ACCESSION   NZ_CP094861
VERSION     NZ_CP094861.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27160588
            Assembly: GCF_022832895.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Kopczyk,M., Mulroney,K., Chakera,A., Ramsay,J.P., Colombi,E.,
            Coombs,G.W. and Mowlaboccus,S.
  TITLE     Sequencing of Staphylococcus epidermidis isolated from peritoneal
            cavity
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7305)
  AUTHORS   Kopczyk,M., Mulroney,K., Chakera,A., Ramsay,J.P., Colombi,E.,
            Coombs,G.W. and Mowlaboccus,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2022) School of Pharmacy & Biomedical Sciences,
            Curtin University, Kent street, Bentley, WA 6102, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-MAR-2022
            Assembly Method :: Flye v. 2.9-b1774; Racon v. 1.4.15; Pilon v. 1.23
            Assembly Name                     :: C99MK-9-Mar-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1552.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022832895.1-RS_2024_04_19
            Annotation Date                   :: 04/19/2024 10:06:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,425
            CDSs (total)                      :: 2,341
            Genes (coding)                    :: 2,266
            CDSs (with protein)               :: 2,266
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 48 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 27 of 75
            Pseudo Genes (multiple problems)  :: 27 of 75
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP094861.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7305
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="C99"
                     /isolation_source="pertoneal cavity"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="pC99MK2"
                     /geo_loc_name="Australia: Perth"
                     /lat_lon="31.92851574 S 115.86399442 E"
                     /collection_date="2017-04-14"
                     /collected_by="Harry Perkins Institute of Medical Research"
     gene            1..861
                     /locus_tag="MUB20_RS11955"
                     /old_locus_tag="MUB20_11955"
     CDS             1..861
                     /locus_tag="MUB20_RS11955"
                     /old_locus_tag="MUB20_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456287.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_233918546.1"
                     /translation="MSGETVVYQNEMNLVPLRKFTSTEIDIFFSLCNKLKEQDVKEVTI
                     PFEELRHLSNYYHRSQKRFVKDLEHVYDKLLSLTYTERSGLSFKKFVLFTGYEVNVDTQ
                     ELIVSINPKLKHVLNEITADFTKFELKEMTHLKSTYSKNMFRLLKQYKHTGYFKIQIDD
                     FRERLDIPKSYRMTHINQKVLTPIIKELSMIFNNLHISKIKAKKGRKIEWLEFTFDAEK
                     RIHNKRQPQMTNIGKSRQYTNREKTPKWLDEKIYKQSQEIHNEDAKLKQDREAFQRQLE
                     EKWEE"
                     /name="RepB family plasmid replication initiator protein"
     gene            complement(1165..1260)
                     /locus_tag="MUB20_RS11960"
                     /old_locus_tag="MUB20_11960"
     CDS             complement(1165..1260)
                     /locus_tag="MUB20_RS11960"
                     /old_locus_tag="MUB20_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002501808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_002501808.1"
                     /translation="MLAIFVYITVSIFIECCVALFKFWLEQRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(1595..1960)
                     /locus_tag="MUB20_RS11965"
                     /old_locus_tag="MUB20_11965"
     CDS             complement(1595..1960)
                     /locus_tag="MUB20_RS11965"
                     /old_locus_tag="MUB20_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_196309543.1"
                     /translation="MNWLKIIILTLSLVPIEFIGLFTDYQTGLLIGYIPFIIVAILIST
                     SIFKFGLKNNISIIISRCIGIFLSWECVRWFMSHYEPEFYFEPFMADDFALFLGVIHFI
                     VIMLIYLAIYGFSHRNQ"
                     /name="hypothetical protein"
     gene            complement(2074..2442)
                     /locus_tag="MUB20_RS11970"
                     /old_locus_tag="MUB20_11970"
     CDS             complement(2074..2442)
                     /locus_tag="MUB20_RS11970"
                     /old_locus_tag="MUB20_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="WP_196309542.1"
                     /translation="MNKILKILITSIIVVIIILTVWTFSVIIYQKHKSEEIIDNVIKRK
                     GWNKKIKNEKMSFNIMMGYAEKDIIFKDQPHSEYEYNVTPAPAPWTDDKEYKVWGETDL
                     QKKDSYYKYLLESEPYRK"
                     /name="DUF3139 domain-containing protein"
     gene            complement(2446..3150)
                     /locus_tag="MUB20_RS11975"
                     /old_locus_tag="MUB20_11975"
     CDS             complement(2446..3150)
                     /locus_tag="MUB20_RS11975"
                     /old_locus_tag="MUB20_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020368010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_196309541.1"
                     /translation="MKNFAKLILMGILVSGSGIASVQTNIIHAKESHDSTPQNIKLVGT
                     YDTSQVDSKTMKQFKEIEKEDNNFHITKHGNKVVVEDKLPNPENKTSSYSADGSAENNT
                     KVINFSDFVGNMDGKDDGKIPDGITFYSGKSYNGQHDGQKVKKGTHVHCNRFNGTKSDH
                     RYWSKKHPRAYVDFYKSDCWYHAKAYKCSSLGKMTKCDGLNSIYRKGVKDCSSWKGKPK
                     HKNWPKTAWYRN"
                     /name="hypothetical protein"
     gene            3255..3614
                     /locus_tag="MUB20_RS11980"
                     /old_locus_tag="MUB20_11980"
     CDS             3255..3614
                     /locus_tag="MUB20_RS11980"
                     /old_locus_tag="MUB20_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002501804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049387235.1"
                     /translation="MKKVFYGIGIIIFLAICYIFVNFFYFDSWACHSSEKQLNNYVKHE
                     DTKKLKEVAKDNKTYQFLKKQNKISIDGKSDNQGSGHIGYYRIDINGKPAALFIKIKHS
                     FLPEIPKVKTVELDK"
                     /name="hypothetical protein"
     gene            complement(3674..4126)
                     /locus_tag="MUB20_RS11985"
                     /old_locus_tag="MUB20_11985"
     CDS             complement(3674..4126)
                     /locus_tag="MUB20_RS11985"
                     /old_locus_tag="MUB20_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002958643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_196309540.1"
                     /translation="MPKKQSSKEKILTVRVSQAQYKKIQDLAYIRSMNVTEYIRQTAVG
                     NRIKPTVIEYPKEQTTIDDYNSNNDTNDWHEIIEQLKNDNETLKSNNQELQQHIHQLED
                     EIDPMRQENDVFHHLLQHFDSTAFMNFNTYRDDRPLKNAIKRLKER"
                     /name="hypothetical protein"
     gene            complement(5463..5681)
                     /locus_tag="MUB20_RS11990"
                     /old_locus_tag="MUB20_11990"
     CDS             complement(5463..5681)
                     /locus_tag="MUB20_RS11990"
                     /old_locus_tag="MUB20_11990"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_196309538.1"
                     /translation="MRNFSEKEIDKYIEFVDEEMIDISEIQGQCHICGKPLKDLKLPKG
                     PEKNVVCYNDLDVFIETIEELEEENLL"
                     /name="hypothetical protein"
     gene            <6121..6270
                     /locus_tag="MUB20_RS11995"
                     /old_locus_tag="MUB20_11995"
                     /pseudo=""
     CDS             <6121..6270
                     /locus_tag="MUB20_RS11995"
                     /old_locus_tag="MUB20_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495128.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(6667..>6834)
                     /locus_tag="MUB20_RS12155"
                     /pseudo=""
     CDS             complement(6667..>6834)
                     /locus_tag="MUB20_RS12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002494720.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /name="TIGR00730 family Rossman fold protein"
     Typing          2..775
                     /name="rep_cluster_2222"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.046"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 atgtctggag aaactgttgt atatcaaaac gaaatgaatt tagttccttt acgtaaattt
       61 acaagtacag aaattgatat tttttttagt ctttgcaata aactcaaaga acaagatgta
      121 aaagaagtta ctattccatt tgaagaatta cgtcatttaa gtaattacta tcaccgttct
      181 caaaaaagat ttgttaaaga tttagaacat gtttatgata aattgctaag tttaacttac
      241 acagaacgaa gtggtttatc atttaaaaaa tttgtattat ttactggtta tgaagttaat
      301 gtagatacac aagaacttat cgttagtatt aatcctaaat taaaacatgt tttaaacgag
      361 ataacagcag atttcactaa attcgaatta aaagaaatga cacaccttaa atctacctat
      421 tctaaaaata tgtttagact tctaaaacaa tataagcata caggttattt taaaatacaa
      481 attgatgatt ttagagaacg cttagatatt ccgaaaagtt atcgcatgac acatattaat
      541 caaaaagttt taactccaat tataaaagaa ctttctatga tatttaataa tcttcatatt
      601 agtaaaatca aagctaaaaa aggacgtaaa attgaatggt tagaatttac ctttgacgct
      661 gagaaacgca ttcacaacaa gcgacaacca caaatgacta atataggtaa gtcgcgccaa
      721 tataccaatc gtgaaaaaac acctaaatgg ttagacgaaa agatatataa acaatctcaa
      781 gagatacata atgaagatgc aaaattaaaa caagatcgag aggcctttca acgtcaatta
      841 gaagaaaaat gggaggaata ataatctaaa tgagctacat ctatatttta cttttatttc
      901 ctttaatagt gggctttttc ttatcaataa aaaagaaaaa gtatgataaa aaataattaa
      961 ttcactattg taaagaaatt acaaattttc atgagtaaga cttgtaataa aaatacagaa
     1021 ttttatgagt aaaacttgta attttattgc aagtgaagaa gttacaagat atactgaaac
     1081 taacaaatat tgctaacatt tacgcaccaa accccagcct attgcagtag gctggggttt
     1141 ttcttcggtg ttggctacgc cgtcttattt tttattacgt tgctctagcc aaaatttaaa
     1201 caacgcaaca cagcattcta taaaaataga tactgttata taaacaaata tcgctaacat
     1261 ttcatcgcac cccctctcta cgtcaatttg acgtctgaga gatagacgac ctcaatagta
     1321 taacagaatc tgattttaat agattctccg tttttgaata aagatacaaa gtgatcgtaa
     1381 atgaaaaagt atgaaaacgc gtggttaagg gcatgtataa aatatgaata atcgataatc
     1441 caaaaacgaa gggacaaacg ctgaaatgac ggcgtttgtc cctttgtgtt aagttgacat
     1501 aattaactat tataggtagt ggaattgtat aaatactaaa aaacacaaac attgttaaat
     1561 taatctttgt gtcctttaat aagaaacata ataattactg attacgatga gaaaaaccat
     1621 agatagctaa atatataagc ataataacta tgaaatgaat aactcctaaa aataatgcaa
     1681 aatcgtcagc cataaaaggt tcaaagtaaa attccggctc ataatggctc ataaaccaac
     1741 gaacacattc ccaagaaaga aatattccaa tgcatcgact tattataata cttatattat
     1801 tttttaagcc gaatttaaaa atagatgtac ttattaatat agcaactatt ataaatggga
     1861 tgtaacctat taataaacct gtttgataat cagtaaataa tccgataaat tctattggaa
     1921 ctaaagataa agttaatata atgattttta accagttcat tttaaaattt ctcttttcaa
     1981 tatcatttta tttaacataa ttaggtagaa gtctacagcg tctttaggtg gtagatgaat
     2041 gccttctatt ttttattatg ttatacattt atattatttt ctgtaaggtt ctgattctaa
     2101 aagatattta taataggagt ctttcttttg taaatctgtt tctccccaca ccttatattc
     2161 tttatcatct gtccatggtg ctggtgctgg tgtcacgtta tactcatact cactatgtgg
     2221 ttgatcttta aaaataatat ctttttcagc atatcccatc ataatattaa agctcatttt
     2281 ttcgtttttt atttttttat tccaaccctt acgttttata acgttatcga taatttcttc
     2341 actcttatgt ttctgataaa taatcacact aaaagtccaa actgttaaga taataatgac
     2401 aacaataata gaagttatta atatttttaa gattttattc attttttaat ttctatacca
     2461 tgctgtttta ggccagtttt tatgtttagg tttacctttc catgatgagc aatctttgac
     2521 accttttcta taaatactat tcaaaccatc gcatttagtc atttttccca aggaagaaca
     2581 tttataagct ttggcgtgat accagcaatc acttttataa aaatctacat aagctctagg
     2641 atgttttttt gaccagtatc tatgatcaga ttttgttccg ttaaatctat tacaatgtac
     2701 atgagtccct ttttttactt tttgaccatc gtgttgtccg ttatatgatt taccactata
     2761 aaaggttatc ccatccggta tttttccatc atctttccca tccatattac caacaaaatc
     2821 agagaaatta attacttttg tattattttc agcactacca tcagctgaat aacttgaagt
     2881 tttattctct ggattaggta atttgtcttc tacaacgact ttatttccat gtttagttat
     2941 gtggaaatta ttatcttctt tttctatttc tttaaattgt ttcatcgttt tggaatcaac
     3001 ttgagaagta tcatacgttc ccactaattt aatattttga ggagttgaat cgtgactttc
     3061 ttttgcgtga attatatttg tttgtacact cgctatccct gaccccgata ctaaaatgcc
     3121 catcaaaatt agttttgcga aatttttcaa cttttttaca tctccttgaa aattaaatat
     3181 attatgtaga aaaaatatac acttttaagt tatcataaat tataaaaatt tttttgaaaa
     3241 ctaggtgaac tttaatgaaa aaagtattct atggcattgg tataataatt tttttagcaa
     3301 tttgttatat ttttgtaaac tttttctatt ttgattcatg ggcgtgtcat tcaagtgaaa
     3361 agcaattaaa taattatgtt aagcatgaag atactaagaa attaaaagag gttgctaaag
     3421 ataataaaac gtatcaattc ttaaaaaagc aaaataaaat ttctatagat ggaaaatctg
     3481 ataatcaagg aagtgggcat ataggttatt atcgtattga tatcaatgga aaaccagctg
     3541 cattatttat aaaaattaag cattcatttc ttccagaaat accaaaggta aaaacagtag
     3601 agttagataa ataaaatatc tgaaaagaac gataaaaagc aagcgactaa tatgattatt
     3661 agtcgcttgc tttttatcgt tctttcaatc gtttaatcgc attttttaat gggcgatcat
     3721 cacgatatgt attgaagttc ataaatgctg tactatcaaa atgttgtaat aaatgatgaa
     3781 aaacgtcatt ttcttgtctc attgggtcta tctcatcttc cagctgatga atatgttgct
     3841 gaagttcttg attgttagat ttgagtgtct cgttatcgtt cttcagctgt tcaatgattt
     3901 catgccaatc attcgtatca ttattagaat tataatcatc tatcgttgtt tgttcttttg
     3961 gatattcaat gaccgtcggt ttaatccgat tacccacagc tgtttggcgt atatattctg
     4021 tgacattcat actacgtatg taggctaaat cttgtatctt tttgtattgc gcttgactga
     4081 cacgaacggt taagattttt tctttcgaac tttgcttttt tggcatttaa tcacctcata
     4141 attaatatat aataagcata caggtgtgtg cttatattta tattaatata acatacatat
     4201 gaatgctaat atatgtgtat atttcgaggt gttatgagat aacaaatgac atacacttaa
     4261 tcaaacaaat atatttaatg aagtgtatgt catttgttag ctatataacg aaattcaaaa
     4321 gtatactttt gaatgcagtg aggacctcgc cagagctcga tatactctta ctgtaaaaca
     4381 gactcacatt tttgaggcgt cactttgaga gtatccgtca ttgtccatca acgaagtatt
     4441 gtcgaaagta gagaaccaca agcaaatcaa aaacgatgag attgatttga aacatacaaa
     4501 tttaaatttt gattgggttg aaaatcaaaa taacttgtac aattgtgtga aagatagagt
     4561 cgatgactat caagaacagc gatgaattgc caaacagtta atgagataca tgacgcgtta
     4621 atagactatc tatgccagca tggctttgat gttgaacgta gggagacgaa aaagcatatt
     4681 caattacagc aatttggaat ttcgaaaagc aaaattactt cttaaaaaat ttgtaatgct
     4741 gtctaaatat aataatattt tttgtaagga tttcataaaa atgtatacca aatggctcca
     4801 tttttatgtc tccattcggt agccattttt aatttttggg atctattcag tatcttttta
     4861 gtatacattc ggtctccata taacaaattt catcgcataa ataggaatcc tatttgacac
     4921 tgtcaaatca atgcttttaa caaaaatggc taccgaatgg agactttttg tgcaggattc
     4981 ctcttatgct ctttaaaata caaaagttac attatacacc tacttttcag gttttatctt
     5041 ttttctaaaa agaacaattg agataaaaaa tatatttctc tttctgaaag tgttgagaaa
     5101 tgaattttaa agtgatttat agcctctctg ttgaacgaaa gaaatttttg tttgttttta
     5161 tattcgaaat gattaaatgt cctagaaagg gcttaaaatg caaaataaaa aaaccactca
     5221 aaaatgagtg gtctggagaa tagctataat gctgtgcaat taaaaatgag cactgtattg
     5281 agcataccac acgcatttat gccgagaaaa tttattgatg ttgagaagaa cccttaacta
     5341 aacttgcaga cgaatgtctg tatagcgtga gctattaaac cggccattcg acaagttttg
     5401 ggattgttaa gggttccgag gctcaacgtc aataaagcaa ttggaataaa aattagattg
     5461 gtttagagca aattttcttc ttctaattcc tctattgttt caataaatac gtctaaatca
     5521 ttgtagcaaa ctacattctt ttctggtcct tttggtagtt ttaaatcttt taaaggttta
     5581 ccgcatatat ggcattgacc ttgtatttcg cttatgtcta tcatctcttc atcaacaaac
     5641 tctatgtatt tatctatttc tttttcgcta aaattacgca tttcgatact cctttttaga
     5701 aacgttttta gtgagtttct tcttgtcttg atactatata gaaataacgt tcttttttat
     5761 attcatctta aaacactgat tttacggcgt ttttaacaat ttttggtgta taaataaaaa
     5821 tttgacataa aaaaagatcg gtcatattag atcaagtttt tagacagtgt tatatttata
     5881 ataaaattaa aaaagaagtc gcccactccc cgaccaaagt ttgtgaatgc gacttacctt
     5941 aaagaaaaac agttgatgtt tttgttttta atttgtatat ctagatatta aatgatatta
     6001 gtttattctt caagatatat atttggttga gcgacttctt aaattaaagt aaggagtcga
     6061 ttttttatga gcaaaaaaca tcagaaaatc ttttcaatct ttggtttaag tgagtgtgag
     6121 ttgaatgatt atttgctgga attaacaatg aatgaaaata aggtaactaa aaaagacttt
     6181 gaagagtttt tatgtagtat aagaaatgtt cctgaaattc cgaatagaca agttaatgat
     6241 attttggaag gttataaaaa tagtgattaa taaaaaaccc cttagcattt actcgctaag
     6301 ggatttttga cttacaagaa gaaatcaaaa attctagtaa gcttaattaa cttattaaga
     6361 gctatcgtaa ctttaattat agaattacta agataattct gtaaggagga gcccaatctc
     6421 ctccttcaat tttattgtcg ataatgcaat tggaataaag cattaaaaat agcactcaat
     6481 ttatgaacaa ctatgtgttg gggaagattt acttcacatt aaaagactaa atgttgaatg
     6541 aggataatgc tatattctta ctttaacact tatttatttt cacctcatcc aaaaaccatt
     6601 taacaattca acaagtttag cgattgttaa gagctcggag gttcaacgtt aataaagcaa
     6661 ttttgattaa taagttctta cttttggtgc tttatagttt tctataaatt cttcaatttc
     6721 ttggaatgag tttgagaata gtattttatc tctatcttct taagttaaaa actcttttga
     6781 aaccatttga ttgtagaatt gttcgataag agaataataa ttatttgtgt tgaaaatatc
     6841 attaaataaa gaattgcaat tagtttagaa aaaagctttt aataagttaa ttaagagcaa
     6901 taataacttt aattactaaa ttagtagatt agttcagtca atctatcttt atttagcatg
     6961 gctttgaatt ttatgatggg agagacccct gaccttttta atatattttt tataaatctt
     7021 tttaaaacct ttttaggtac tgtttgcccc ttagattgac aaggctttta gaatgttaaa
     7081 ggcaactttt tgtaactaca aaggcaactt tttgtaacta caaaggcaac tttttgtaac
     7141 tataaaggca actttttgta actacaaagg caactttttt attttaagtt tcctttgtag
     7201 ttacaagttt gataatataa tgttcgtaaa gataaatata ttatttatga gaaacctaaa
     7261 taatattaaa gtgtccttac aatgtacatg ggagtgtgtc aaatt
//
