LOCUS       NZ_CP094776             4670 bp    DNA     circular CON 20-OCT-2023
DEFINITION  Staphylococcus aureus strain IVB6191 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP094776
VERSION     NZ_CP094776.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26923335
            Assembly: GCF_025559585.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Akarsu,H., Liljander,A., Younan,M., Brodard,I., Glucks,I.,
            Labroussaa,F., Overesch,G., Kuhnert,P., Perreten,V., Drexler,J.F.,
            Corman,V.M., Falquet,L. and Jores,J.
  TITLE     Comparative Genomics of East African Camel-Associated
            Staphylococcaceae spp.: Diversity and Inheritance of Traits Involved
            in Host-Pathogen Interactions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Akarsu,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2022) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 671x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/20/2023 05:17:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,698
            Genes (coding)                    :: 2,584
            CDSs (with protein)               :: 2,584
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 61 of 114
            Pseudo Genes (incomplete)         :: 39 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 15 of 114
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP094776.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="IVB6191"
                     /isolation_source="milk"
                     /host="Cattle"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed1"
                     /geo_loc_name="Kenya"
                     /collection_date="2009"
                     /collected_by="Joerg Jores, Anne Liljander, Mario Younan,
                     Jan Felix Drexler, Victor Max Corman, Ilona Glucks"
     gene            <3..1172
                     /gene="tet"
                     /locus_tag="MUA69_RS13860"
                     /old_locus_tag="MUA69_13835"
                     /pseudo=""
     CDS             <3..1172
                     /gene="tet"
                     /locus_tag="MUA69_RS13860"
                     /old_locus_tag="MUA69_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492283.1"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tet(L)/Tet(K)/Tet(45) family tetracycline efflux
                     MFS transporter"
                     /name="tet"
     gene            1359..2600
                     /gene="mobV"
                     /locus_tag="MUA69_RS13865"
                     /old_locus_tag="MUA69_13840"
     CDS             1359..2600
                     /gene="mobV"
                     /locus_tag="MUA69_RS13865"
                     /old_locus_tag="MUA69_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_262634552.1"
                     /translation="MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNY
                     DLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFF
                     EYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALTAFQ
                     DRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYKRESQKTDHIKQKND
                     KLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQKQIKAA
                     QDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIE
                     IALKLLKILLKELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQ
                     EQQKNQKRDRGMHL"
                     /name="mobV"
     gene            2787..2966
                     /locus_tag="MUA69_RS13870"
                     /old_locus_tag="MUA69_13845"
     CDS             2787..2966
                     /locus_tag="MUA69_RS13870"
                     /old_locus_tag="MUA69_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000042035.1"
                     /translation="MSKNLDKGLVISTVLNVILCMLLILTMRVESLTVSVILAIITGVN
                     AVYLVAKVLKQKKT"
                     /name="hypothetical protein"
     gene            3185..4129
                     /locus_tag="MUA69_RS13875"
                     /old_locus_tag="MUA69_13850"
     CDS             3185..4129
                     /locus_tag="MUA69_RS13875"
                     /old_locus_tag="MUA69_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_000042122.1"
                     /translation="MSKNNHTNHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            4291..>4668
                     /locus_tag="MUA69_RS13880"
                     /old_locus_tag="MUA69_13855"
                     /pseudo=""
     CDS             4291..>4668
                     /locus_tag="MUA69_RS13880"
                     /old_locus_tag="MUA69_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492283.1"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /name="MFS transporter"
     ARG             4292..4668
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="99.21"
                     /coverage="27.45"
     ARG             4..1172
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="100.0"
                     /coverage="84.75"
     Typing          3191..4129
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.574"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1360..2597
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.758"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaaaattatc tgattatata aatataaaaa aattgttaat tattggtatt agtttgagct
       61 gtcttggttc attgattgct tttattggtc acaatcactt ttttattttg atttttggta
      121 ggttagtaca aggagtagga tctgctgcat tcccttcact gattatggtg gttgtagcta
      181 gaaatattac aagaaaaaaa caaggcaaag cctttggttt tataggatca attgtagctt
      241 taggtgaagg gttaggtcct tcaatagggg gaataatagc acattatatt cattggtctt
      301 acctacttat acttcctatg attacaatag taactatacc ttttcttatt aaagtaatgg
      361 tacctggtaa atcaacaaaa aatacattag atatcgtagg tattgtttta atgtctataa
      421 gtattatatg ttttatgtta tttacgacaa attataattg gactttttta atactcttca
      481 caatcttttt tgtgattttt attaaacata tttcaagagt ttctaaccct tttattaatc
      541 ctaaactagg gaaaaacatt ccgtttatgc ttggtttgtt ttctggtggg ctaatatttt
      601 ctatagtagc tggttttata tcaatggtgc cttatatgat gaaaactatt tatcatgtaa
      661 atgtagcgac aataggtaat agtgttattt ttcctggaac catgagtgtt attgtttttg
      721 gttattttgg tggtttttta gtggatagaa aaggatcatt atttgttttt attttaggat
      781 cattgtctat ctctataagt tttttaacta ttgcattttt tgttgagttt agtatgtggt
      841 tgactacttt tatgtttata tttgttatgg gcggattatc ttttactaaa acagttatat
      901 caaaaatagt atcaagtagt ctttctgaag aagaagttgc ttctggaatg agtttgctaa
      961 atttcacaag ttttttatca gagggaacag gtatagcaat tgtaggaggt ttattgtcac
     1021 tacaattgat taatcgtaaa ctagttctgg aatttataaa ttattcttct ggagtgtata
     1081 gtaatattct tgtagccatg gctatcctta ttattttatg ttgtcttttg acgattattg
     1141 tatttaaacg ttctgaaaag cagtttgaat agttatatta tattttggtt tagaactatg
     1201 agtggctagc attttgccac tcattttttg cgttagcaaa aacaggttta agcctcgcag
     1261 agcacacgta ttaacgactt attgtaaaat aagtctagtg tgttagactt aaactattaa
     1321 atacacatga aacctttgtg cttaggagtg atttttatat gtcttattcc attgttagag
     1381 tttcaaaagt taaatctgga acaaatacaa cgggcataca aaaacatgtt caaagagaaa
     1441 ataataatta tgaaaatgaa gatatagacc atagtaaaac ttacttaaat tatgatttgg
     1501 taaatgctaa taaacagaat tttaataact tgattgatga aaaaatcgaa cagaattata
     1561 caggcaaaag aaaaattaga acagacgcga ttaaacacat tgatggttta attacatcag
     1621 acaatgattt ctttgataat caaacgccag aagatacaaa gcagtttttt gaatatgcta
     1681 aagagttttt agaacaagaa tacggtaaag ataatttatt atatgcaaca gttcacatgg
     1741 acgaaaaaac accacatatg cattatggcg ttgttccaat aactgatgat ggtcgtttaa
     1801 gtgctaaaga agttgtaggt aataaaaaag ctttaacagc gtttcaagat agatttaatg
     1861 agcatgttaa acaacgagga tatgatttag aacgtgggca atcaagacaa gtaacaaatg
     1921 ctaaacatga gcaaataagt cagtataaac aaaaaacaga atatcataag caagaatata
     1981 aacgtgagag ccaaaaaaca gaccatataa agcaaaagaa cgataaatta atgcaagagt
     2041 accaaaaatc gttaaatacg cttaaaaagc ctataaatgt tccgtatgag caagaaactg
     2101 aaaaagtagg tggtttattt agcaaagaaa tacaagaaac tggaaatgtt gtaataagcc
     2161 aaaaagattt caatgaattt cagaaacaga taaaagctgc tcaagatatt tcggaagatt
     2221 acgagtatat aaagtctggt agagccttag atgataaaga taaggaaata cgagagaaag
     2281 atgatttatt aaataaagca gttgagcgta ttgaaaacgc agacgataat tttaaccaac
     2341 tttacgaaaa tgcaaagcca cttaaagaga atatagaaat agcgttaaag cttttaaaaa
     2401 tcttactaaa agagttagaa cgagttttag gaagaaatac ctttgcggaa agagttaata
     2461 agttaacaga agatgaacca aaactaaatg gtttagcagg aaacttagat aaaaaaatga
     2521 atccagaatt atattcagaa caggaacagc aacaagaaca acaaaagaat caaaaacgag
     2581 atagaggtat gcacttatag aacatgcatt tatgccgaga aaacttattg gttggaatgg
     2641 gctatgtgtt agctaacttg ttagcgagtt ggttggactt gaattgggat taatcccaag
     2701 aaagtaccaa ctcaacaaca cataaagccc tgtaggttcc gaccaataag gaaattggaa
     2761 taaagcaata aaaggagttg aagaagatga gtaaaaattt agataaggga ttggttatat
     2821 caacagtttt aaacgttatt ctttgcatgc ttttaatatt aactatgcga gtagaaagtt
     2881 taactgtatc agttatatta gcaataatca ctggagtaaa tgcagtatat ttagttgcta
     2941 aggttttaaa acagaaaaag acctgatctg attagaccaa gtcttttgat agtgttatat
     3001 taataaacaa ataaaaaaga agtcgctcac tccctgacca aagtttgtga tagcgactta
     3061 cccaaagaaa aaacactaag ttgttttttt atattttgta tatctagata ttaaacgata
     3121 tttaaatata catcaagata tatattcggg tgagcgactt cttaaattaa ggagtcgatt
     3181 ttttatgagt aaaaataatc atacaaatca ttcaaatcac ttagaaaatc acgatttaga
     3241 caatttttct aaaaccggct actctaatag ccggttggac gcacatactg tgtgcatatc
     3301 tgatccaaaa ttaagttttg atgcaatgac gatcgttgga aatctcaacc gagacaacgc
     3361 tcaagccctt tctaaattta tgagtgtaga gccccaaata agactttggg atattcttca
     3421 aacaaagttt aaagctaaag cacttcaaga aaaagtttat attgaatatg acaaagtgaa
     3481 agcagatagt tgggatagac gtaatatgcg tattgaattt aatccaaaca aacttacacg
     3541 agatgaaatg atttggttaa aacaaaatat aataagctac atggaagatg acggttttac
     3601 aagattagat ttagcctttg attttgaaga tgatttgagt gactactatg caatgtctga
     3661 taaagcagtt aagaaaacta ttttttatgg tcgtaatggt aagccagaaa caaaatattt
     3721 tggcgtgaga gatagtaata gatttattag aatttataat aaaaagcaag aacgtaaaga
     3781 taatgcagat gctgaagtta tgtctgaaca tttatggcgt gtagaaatcg aacttaaaag
     3841 agatatggtg gattactgga atgattgctt tagtgattta catatcttgc aaccagattg
     3901 gaaaactatc caacgcactg cggatagagc aatagttttt atgttattga gtgatgaaga
     3961 agaatgggga aagcttcaca gaaattctag aacaaaatat aagaatttga taaaagaaat
     4021 ttcgccagtc gatttaacgg acttaatgaa atcgacttta aaagcgaacg aaaaacaatt
     4081 gcaaaaacaa atcgattttt ggcaacatga atttaaattt tggaaatagt gtacatatta
     4141 atattactga acaaaaatga tatatttaaa ctattctaat ttaggaggat ttttttatga
     4201 agtgtctatt taaaaatttg gggaatttat atgaggtgaa agaataattt acccctataa
     4261 actttagtca cctcaagtaa agaggtaaaa ttgtttagtt tatataaaaa atttaaaggt
     4321 ttgtttttta gcgttttatt ttggctttgt attctttcat tttttagtgt attaaatgaa
     4381 atggttttaa atgtttcttt acctgatatt gcaaatcatt ttaatactac tcctggaatt
     4441 acaaactggg taaacactgc atatatgtta actttttcga taggaacagc agtatatgga
     4501 aaattatctg attatataaa tataaaaaaa ttgttaatta ttggtattag tttgagctgt
     4561 cttggttcat tgattgcttt tattggtcac aatcactttt ttattttgat ttttggtagg
     4621 ttagtacaag gagtaggatc tgctgcattc ccttcactga ttatggtggt
//
