LOCUS       NZ_CP094762             4711 bp    DNA     circular CON 20-OCT-2023
DEFINITION  Staphylococcus aureus strain IVB6232 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP094762
VERSION     NZ_CP094762.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26923342
            Assembly: GCF_025559225.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Akarsu,H., Liljander,A., Younan,M., Brodard,I., Glucks,I.,
            Labroussaa,F., Overesch,G., Kuhnert,P., Perreten,V., Drexler,J.F.,
            Corman,V.M., Falquet,L. and Jores,J.
  TITLE     Comparative Genomics of East African Camel-Associated
            Staphylococcaceae spp.: Diversity and Inheritance of Traits Involved
            in Host-Pathogen Interactions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Akarsu,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2022) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 696x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/20/2023 06:14:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,693
            CDSs (total)                      :: 2,611
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 43 of 99
            Pseudo Genes (incomplete)         :: 38 of 99
            Pseudo Genes (internal stop)      :: 35 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP094762.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="IVB6232"
                     /isolation_source="Pus swab"
                     /host="Camel"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed3"
                     /geo_loc_name="Kenya:Laikipia"
                     /collection_date="2014"
                     /collected_by="Joerg Jores, Anne Liljander, Mario Younan,
                     Jan Felix Drexler, Victor Max Corman, Ilona Glucks"
     gene            <2..1105
                     /gene="tet"
                     /locus_tag="MUA14_RS13450"
                     /old_locus_tag="MUA14_13425"
                     /pseudo=""
     CDS             <2..1105
                     /gene="tet"
                     /locus_tag="MUA14_RS13450"
                     /old_locus_tag="MUA14_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492283.1"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tet(L)/Tet(K)/Tet(45) family tetracycline efflux
                     MFS transporter"
                     /name="tet"
     gene            1291..2532
                     /gene="mobV"
                     /locus_tag="MUA14_RS13455"
                     /old_locus_tag="MUA14_13430"
     CDS             1291..2532
                     /gene="mobV"
                     /locus_tag="MUA14_RS13455"
                     /old_locus_tag="MUA14_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000122374.1"
                     /translation="MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNY
                     DLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFF
                     EYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALTAFQ
                     DRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERESQKTDHIKQKND
                     KLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQKQIKAA
                     QDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIE
                     IALKLLKILLKELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQ
                     EQQKNQKRDRGMHL"
                     /name="mobV"
     gene            3059..4003
                     /locus_tag="MUA14_RS13460"
                     /old_locus_tag="MUA14_13435"
     CDS             3059..4003
                     /locus_tag="MUA14_RS13460"
                     /old_locus_tag="MUA14_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_000277654.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            4165..>4710
                     /locus_tag="MUA14_RS13465"
                     /old_locus_tag="MUA14_13440"
                     /pseudo=""
     CDS             4165..>4710
                     /locus_tag="MUA14_RS13465"
                     /old_locus_tag="MUA14_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492283.1"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /name="MFS transporter"
     ARG             4166..4710
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="100.0"
                     /coverage="39.65"
     ARG             3..1105
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="100.0"
                     /coverage="79.96"
     Typing          3060..4003
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1292..2529
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttcattgatt gcttttattg gtcacaatca cttttttatt ttgatttttg gtaggttagt
       61 acaaggagta ggatctgctg cattcccttc actgattatg gtggttgtag ctagaaatat
      121 tacaagaaaa aaacaaggca aagcctttgg ttttatagga tcaattgtag ctttaggtga
      181 agggttaggt ccttcaatag ggggaataat agcacattat attcattggt cttacctact
      241 tatacttcct atgattacaa tagtaactat accttttctt attaaagtaa tggtacctgg
      301 taaatcaaca aaaaatacat tagatatcgt aggtattgtt ttaatgtcta taagtattat
      361 atgttttatg ttatttacga caaattataa ttggactttt ttaatactct tcacaatctt
      421 ttttgtgatt tttattaaac atatttcaag agtttctaac ccttttatta atcctaaact
      481 agggaaaaac attccgttta tgcttggttt gttttctggt gggctaatat tttctatagt
      541 agctggtttt atatcaatgg tgccttatat gatgaaaact atttatcatg taaatgtagc
      601 gacaataggt aatagtgtta tttttcctgg aaccatgagt gttattgttt ttggttattt
      661 tggtggtttt ttagtggata gaaaaggatc attatttgtt tttattttag gatcattgtc
      721 tatctctata agttttttaa ctattgcatt ttttgttgag tttagtatgt ggttgactac
      781 ttttatgttt atatttgtta tgggcggatt atcttttact aaaacagtta tatcaaaaat
      841 agtatcaagt agtctttctg aagaagaagt tgcttctgga atgagtttgc taaatttcac
      901 aagtttttta tcagagggaa caggtatagc aattgtagga ggtttattgt cactacaatt
      961 gattaatcgt aaactagttc tggaatttat aaattattct tctggagtgt atagtaatat
     1021 tcttgtagcc atggctatcc ttattatttt atgttgtctt ttgacgatta ttgtatttaa
     1081 acgttctgaa aagcagtttg aatagttata ttatattttg gtttagaact atgagtggct
     1141 agcattttgc cactcatttt ttgcgttagc aaaaacaggt ttaagcctcg cagagcacac
     1201 gtattaacga cttattaaaa ataagtctag tgtgttagac ttaaactatt aaatacacat
     1261 gaaacctttg tgcttaggag tgatttttat atgtcttatt ccattgttag agtttcaaaa
     1321 gttaaatctg gaacaaatac aacgggcata caaaaacatg ttcaaagaga aaataataat
     1381 tatgaaaatg aagatataga ccatagtaaa acttacttaa attatgattt ggtaaatgct
     1441 aataaacaga attttaataa cttgattgat gaaaaaatcg aacagaatta tacaggcaaa
     1501 agaaaaatta gaacagacgc gattaaacac attgatggtt taattacatc agacaatgat
     1561 ttctttgata atcaaacgcc agaagataca aagcagtttt ttgaatatgc taaagagttt
     1621 ttagaacaag aatacggtaa agataattta ttatatgcaa cagttcacat ggacgaaaaa
     1681 acaccacata tgcattatgg cgttgttcca ataactgatg atggtcgttt aagtgctaaa
     1741 gaagttgtag gtaataaaaa agctttaaca gcgtttcaag atagatttaa tgagcatgtt
     1801 aaacaacgag gatatgattt agaacgtggg caatcaagac aagtaacaaa tgctaaacat
     1861 gagcaaataa gtcagtataa acaaaaaaca gaatatcata agcaagaata tgaacgtgag
     1921 agccaaaaaa cagaccatat aaagcaaaag aacgataaat taatgcaaga gtaccaaaaa
     1981 tcgttaaata cgcttaaaaa gcctataaat gttccgtatg agcaagaaac tgaaaaagta
     2041 ggtggtttat ttagcaaaga aatacaagaa actggaaatg ttgtaataag ccaaaaagat
     2101 ttcaatgaat ttcagaaaca gataaaagct gctcaagata tttcggaaga ttacgagtat
     2161 ataaagtctg gtagagcctt agatgataaa gataaggaaa tacgagagaa agatgattta
     2221 ttaaataaag cagttgagcg tattgaaaac gcagacgata attttaacca actttacgaa
     2281 aatgcaaagc cacttaaaga gaatatagaa atagcgttaa agcttttaaa aatcttacta
     2341 aaagagttag aacgagtttt aggaagaaat acctttgcgg aaagagttaa taagttaaca
     2401 gaagatgaac caaaactaaa tggtttagca ggaaacttag ataaaaaaat gaatccagaa
     2461 ttatattcag aacaggaaca gcaacaagaa caacaaaaga atcaaaaacg agatagaggt
     2521 atgcacttat agaacatgca tttatgccga gaaaacttat tggttggaat gggctatgtg
     2581 ttagctaact tgttagcgag ttggttggac ttgaattggg attaatccca agaaagtacc
     2641 aactcaacaa cacataaagc cctgtaggtt ccgaccaata aggaaattgg aataaagcaa
     2701 taaaaggagt tgaagaaatg aaattcagag aagcctttga gaattttata acaagtaagt
     2761 atgtacttgg tgttttagta gttttaactg tttaccagat aatacaaatg cttaaataaa
     2821 aaaagacttg atctgattag accaagtctt ttgatagtgt tatattaata acaaaataaa
     2881 aaggagtcgc tcacgccctg accaaagttt gtgaactaca tcattcaaag aaaaaaacac
     2941 tgagttgttt ttataatctt gtatatttag atattaaacg atatttaaat atacatcaag
     3001 atatatattt gggtgagcga ttccttaaac gaaattgaga ttaaggagtc gattttttat
     3061 gtataaaaac aatcatgcaa atcattcaaa tcatttggaa aatcacgatt tagacaattt
     3121 ttctaaaacc ggctactcta atagccggtt ggacgcacat actgtgtgca tatctgatcc
     3181 aaaattaagt tttgatgcaa tgacgatcgt tggaaatctc aaccgagaca acgctcaagc
     3241 cctttctaaa tttatgagtg tagagcccca aataagactt tgggatattc ttcaaacaaa
     3301 gtttaaagct aaagcacttc aagaaaaagt ttatattgaa tatgacaaag tgaaagcaga
     3361 tagttgggat agacgtaata tgcgtattga atttaatcca aacaaactta cacgagatga
     3421 aatgatttgg ttaaaacaaa atataataag ctacatggaa gatgacggtt ttacaagatt
     3481 agatttagcc tttgattttg aagatgattt gagtgactac tatgcaatgt ctgataaagc
     3541 agttaagaaa actatttttt atggtcgtaa tggtaagcca gaaacaaaat attttggcgt
     3601 gagagatagt aatagattta ttagaattta taataaaaag caagaacgta aagataatgc
     3661 agatgctgaa gttatgtctg aacatttatg gcgtgtagaa atcgaactta aaagagatat
     3721 ggtggattac tggaatgatt gctttagtga tttacatatc ttgcaaccag attggaaaac
     3781 tatccaacgc actgcggata gagcaatagt ttttatgtta ttgagtgatg aagaagaatg
     3841 gggaaagctt cacagaaatt ctagaacaaa atataagaat ttgataaaag aaatttcgcc
     3901 agtcgattta acggacttaa tgaaatcgac tttaaaagcg aacgaaaaac aattgcaaaa
     3961 acaaatcgat ttttggcaac atgaatttaa attttggaaa tagtgtacat attaatatta
     4021 ctgaacaaaa atgatatatt taaactattc taatttagga ggattttttt atgaagtgtc
     4081 tatttaaaaa tttggggaat ttatatgagg tgaaagaata atttacccct ataaacttta
     4141 gtcacctcaa gtaaagaggt aaaattgttt agtttatata aaaaatttaa aggtttgttt
     4201 tatagcgttt tattttggct ttgtattctt tcatttttta gtgtattaaa tgaaatggtt
     4261 ttaaatgttt ctttacctga tattgcaaat cattttaata ctactcctgg aattacaaac
     4321 tgggtaaaca ctgcatatat gttaactttt tcgataggaa cagcagtata tggaaaatta
     4381 tctgattata taaatataaa aaaattgtta attattggta ttagtttgag ctgtcttggt
     4441 tcattgattg cttttattgg tcacaatcac ttttttattt tgatttttgg taggttagta
     4501 caaggagtag gatctgctgc attcccttca ctgattatgg tggttgtagc tagaaatatt
     4561 acaagaaaaa aacaaggcaa agcctttggt tttataggat caattgtagc tttaggtgaa
     4621 gggttaggtc cttcaatagg gggaataata gcacattata ttcattggtc ttacctactt
     4681 atacttccta tgattacaat agtaactata c
//
