LOCUS       NZ_CP094701            13720 bp    DNA     circular CON 20-OCT-2023
DEFINITION  Staphylococcus simulans strain IVB6241 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP094701
VERSION     NZ_CP094701.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26923386
            Assembly: GCF_025558185.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus simulans
  ORGANISM  Staphylococcus simulans
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13720)
  AUTHORS   Akarsu,H., Liljander,A., Younan,M., Brodard,I., Glucks,I.,
            Labroussaa,F., Overesch,G., Kuhnert,P., Perreten,V., Drexler,J.F.,
            Corman,V.M., Falquet,L. and Jores,J.
  TITLE     Comparative Genomics of East African Camel-Associated
            Staphylococcaceae spp.: Diversity and Inheritance of Traits Involved
            in Host-Pathogen Interactions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13720)
  AUTHORS   Akarsu,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2022) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 901x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/20/2023 05:20:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,729
            CDSs (total)                      :: 2,649
            Genes (coding)                    :: 2,615
            CDSs (with protein)               :: 2,615
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 15 of 35
            Pseudo Genes (incomplete)         :: 17 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 2 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP094701.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13720
                     /organism="Staphylococcus simulans"
                     /mol_type="genomic DNA"
                     /strain="IVB6241"
                     /isolation_source="Pus swab"
                     /host="Camel"
                     /db_xref="taxon:1286"
                     /plasmid="unnamed3"
                     /geo_loc_name="Kenya:Laikipia"
                     /collected_by="Joerg Jores, Anne Liljander, Mario Younan,
                     Jan Felix Drexler, Victor Max Corman, Ilona Glucks"
     gene            271..417
                     /locus_tag="MUA31_RS13595"
                     /old_locus_tag="MUA31_13590"
     CDS             271..417
                     /locus_tag="MUA31_RS13595"
                     /old_locus_tag="MUA31_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_199193149.1"
                     /translation="MRFGNYKIDSFWLIMIIGFLATSIFFPFMLLSVVLLLIFGLEKND
                     KKD"
                     /name="hypothetical protein"
     gene            729..830
                     /locus_tag="MUA31_RS13600"
                     /old_locus_tag="MUA31_13595"
     CDS             729..830
                     /locus_tag="MUA31_RS13600"
                     /old_locus_tag="MUA31_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000228874.1"
                     /translation="MVEALVHITTTVISGCIVALFTHWLRTRNNKKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            1395..1595
                     /locus_tag="MUA31_RS13605"
                     /old_locus_tag="MUA31_13600"
     CDS             1395..1595
                     /locus_tag="MUA31_RS13605"
                     /old_locus_tag="MUA31_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_105978674.1"
                     /translation="MIITILLLVLIVNLLESLYLGIKYLRLKKQNAADKEYTKMVEKVA
                     PLMYVTLVISVIALVISWIIS"
                     /name="hypothetical protein"
     gene            complement(1661..1861)
                     /locus_tag="MUA31_RS13610"
                     /old_locus_tag="MUA31_13605"
     CDS             complement(1661..1861)
                     /locus_tag="MUA31_RS13610"
                     /old_locus_tag="MUA31_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017174778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_105978673.1"
                     /translation="MLLDILGYVFGIGFIVFGISALVICGSEVYKVISKSKKKGDYKKS
                     LYFAGFAIICAIALIIMASVF"
                     /name="hypothetical protein"
     gene            complement(2111..2416)
                     /locus_tag="MUA31_RS13615"
                     /old_locus_tag="MUA31_13610"
     CDS             complement(2111..2416)
                     /locus_tag="MUA31_RS13615"
                     /old_locus_tag="MUA31_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002490323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_262547472.1"
                     /translation="MIFIYIIFSAILLYYALKYGIRNGFVELEANKDGLVYYKKSASLL
                     EEIGNIYSRVSTSKSKEAKAIYNEAFDILLSEKKPKIILKELTEKKEEIFKLSIDD"
                     /name="hypothetical protein"
     gene            complement(3064..3282)
                     /locus_tag="MUA31_RS13620"
                     /old_locus_tag="MUA31_13615"
     CDS             complement(3064..3282)
                     /locus_tag="MUA31_RS13620"
                     /old_locus_tag="MUA31_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6007 family protein"
                     /protein_id="WP_105978671.1"
                     /translation="MENLKKSLLSLGWWDLLFTIPMFLLFIYLPTYNFLSIVLNIIIVF
                     FFSMGLVLTTHALWRYMKSTKKFDYKF"
                     /name="DUF6007 family protein"
     gene            complement(3298..3552)
                     /locus_tag="MUA31_RS13625"
                     /old_locus_tag="MUA31_13620"
     CDS             complement(3298..3552)
                     /locus_tag="MUA31_RS13625"
                     /old_locus_tag="MUA31_13620"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_262547473.1"
                     /translation="MQSTAVDNGVTTYAINKAALKWACIFSSYSACIGVSLGAGAAGAM
                     VSGPFGAATGFAGGAACRYIFQTAVEKFGGKDAACKVLS"
                     /name="hypothetical protein"
     gene            complement(3563..4915)
                     /gene="brnQ"
                     /locus_tag="MUA31_RS13630"
                     /old_locus_tag="MUA31_13625"
     CDS             complement(3563..4915)
                     /gene="brnQ"
                     /locus_tag="MUA31_RS13630"
                     /old_locus_tag="MUA31_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450219.1"
                     /GO_function="GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="WP_262547474.1"
                     /translation="MHNKLTWKENMYIGSMLFGLFFGAGNLIFPIHLGQASGPNVILAN
                     IGFLVTAIGLPFLGVIAAGFSQKKDIFAISMKVNRHYAYIFTITLYLVIGPIYALPRLA
                     STSFEVGLSPFISQSLYKPFLFIFSLLFFILTWVFSRNTAKILEYIGKILNPIFVFLLS
                     IILTLSFLQPMGTITSGSISNEYKQTPFFKGFVDGYNTLDALAALAFGMIIVKTIKDMG
                     ISEPLNITRETIKSGAISMVLMALIYSLLAIIGSMSLGEFEPHENGGITLTLIIKYYIG
                     EHGMIFLAVIITIACLKTAIGLNASFSETFSNLFPKFNYIFFTTLSCIVSFLISNTGLT
                     YIIKISTPILLLLYPLAIVLILLEIINFKFFCSKIVYNSTILFTLLAAVIDAIKHCPPF
                     ISDTNLFESITSVFEKYIPLSSIGMGWIPLSIIGFIFGVILRKMLNNYNYR"
                     /name="brnQ"
     gene            complement(5046..5612)
                     /locus_tag="MUA31_RS13635"
                     /old_locus_tag="MUA31_13630"
     CDS             complement(5046..5612)
                     /locus_tag="MUA31_RS13635"
                     /old_locus_tag="MUA31_13630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_107579019.1"
                     /translation="MFLLEFKQFSTRKMVGLFKTEDDVKSWISSIENIKIYKEKIDGRV
                     ITDYFLEYMEIPSYSEINWKGSKFILSKYMFSPEEGDISITWEALNIVDEIEGIISGQS
                     KVGSYVINNDELEDYIKSLEDMKNYLIRYFENKNHNVEVLGHGSEDGEYILVDDKFLCH
                     LEGDTVTKWQEKKSPKSFLDNFNYK"
                     /name="hypothetical protein"
     gene            6130..6261
                     /locus_tag="MUA31_RS13640"
                     /old_locus_tag="MUA31_13635"
     CDS             6130..6261
                     /locus_tag="MUA31_RS13640"
                     /old_locus_tag="MUA31_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="WP_023016136.1"
                     /translation="MSGIVDAISQAVQSGLEQDWVTMGTNIAKALAQGIDVIAGFFG"
                     /name="beta-class phenol-soluble modulin"
     gene            complement(6566..7228)
                     /locus_tag="MUA31_RS13645"
                     /old_locus_tag="MUA31_13640"
     CDS             complement(6566..7228)
                     /locus_tag="MUA31_RS13645"
                     /old_locus_tag="MUA31_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274491.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_023016137.1"
                     /translation="MLKFEKVTKEFQDGNHMIKAVKPTSLSFEKGDFIAIVGPSGSGKS
                     TFLTMAGALQTPTSGEIYINDKKISKMNQKKLSRMRMKEIGFILQATNLVPFLTIKQQF
                     HLLKSYKKDVLSTQEHDKLLDDLGLKEIENKLPSEVSGGQKQRVAIAKAIYTQPSIILA
                     DEPTASLDTENAMAVMKILKEQSKQRQKICIIVTHDERLTKFCDKIFHMEDGYLTQK"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(7241..8290)
                     /locus_tag="MUA31_RS13650"
                     /old_locus_tag="MUA31_13645"
     CDS             complement(7241..8290)
                     /locus_tag="MUA31_RS13650"
                     /old_locus_tag="MUA31_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002466593.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="WP_023016138.1"
                     /translation="MFLAWNEIKYNKLKFGLIIGILVLISYLLFLLSGLANGLINMNTE
                     GIKKWQADAIVLNKDANQTVQQSVITTSDVKGKFKKEAPLKQMGIIASKGNSKENAILF
                     GVTSNSFLIPKIKEGKAFNKDNEVVIDQSLKDKGFKVNDIINLSQSDEKLHIVGVSESA
                     KYNASPVIFTNNKTVEKINPALSSDKTNSVVVKDNNWNDKKINSDLEVIGIDEFVENLP
                     GYKSQNLTMNFMITFLFVISATVIGVFLYVITLQKKSLFGVLKAQGFTDGFLMKMVLAQ
                     TFILSLIGSLIGLMLTLLTSLILPKAVPIQFDVITLIIFGIVLILISLVGSLFSVLSIR
                     KIDPLKAIG"
                     /name="ABC transporter permease"
     gene            8468..9076
                     /locus_tag="MUA31_RS13655"
                     /old_locus_tag="MUA31_13650"
     CDS             8468..9076
                     /locus_tag="MUA31_RS13655"
                     /old_locus_tag="MUA31_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436987.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_107543519.1"
                     /translation="MIVGYARVSSIDQNLERQLANLKTFGVEKIFTEKQSGKSVENRPI
                     FQEALNFVRMGDRFVVESIDRLGRNYDEVINTVNYLKDKEVQLMITSLPMMNEVIGNPL
                     LNKFMKDLIIQILAMVSEQERNESKRRQAQGIKVAKEKGVYKGRPLLYAPNAKDPQKRV
                     IYHRVVEMLEEGQAISKIAKEVNITRQTIYRIKKGNDLV"
                     /name="recombinase family protein"
     gene            complement(9267..10001)
                     /locus_tag="MUA31_RS13660"
                     /old_locus_tag="MUA31_13655"
     CDS             complement(9267..10001)
                     /locus_tag="MUA31_RS13660"
                     /old_locus_tag="MUA31_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_262547470.1"
                     /translation="MKSVKELSEKLEVSKQTVFNNIKRLNIETIKKDNTSFIKNDVDVE
                     KIIQRVTKNKKKYGFESTNTNSEKVKTSNQKNESKHNVQIIELLEKQIDTLNKQLEKQE
                     KRHEVTIEFYRKELQERSKLLENQQVLTLESNKKIQRLESELEEEKQLNYSFDTSAKER
                     QNVNVQEATFTRESKDTDQQQEWEQPTEAQHKDIPEEKEDEIINEEPSTIDDKQFEEID
                     SEYEEREEKPPKKGFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            10397..11353
                     /locus_tag="MUA31_RS13665"
                     /old_locus_tag="MUA31_13660"
     CDS             10397..11353
                     /locus_tag="MUA31_RS13665"
                     /old_locus_tag="MUA31_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_262547471.1"
                     /translation="MSRKNIKNQASQNFYMLHKALFVNEKYKKLSDSAKVTYAILNDRV
                     SLSIKNNWIDENGDIYFIFTNESLQNILDKSKNTITKIKKELQEVGLLEQIRTGFNKPN
                     KLYLHDIETNIGVEKNIQNSSESYDIKESQILGLQNPEIWDSRIPKFGTPESQILDPND
                     TDYIKTDYIKTENNDTDDLHDKKLTYHNDHTHHSNHYNSNFNDEALKFQLLEELPQNIQ
                     NYLNNFSVLEIKIIKSVLLKAKTSFNNSIDSYYLLEDMELEVIYVLKRFKAMLIQKNES
                     VQAMQGYLMTSLKSEFAEMHTLNKRREHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            <11360..11821
                     /locus_tag="MUA31_RS13670"
                     /old_locus_tag="MUA31_13665"
                     /pseudo=""
     CDS             <11360..11821
                     /locus_tag="MUA31_RS13670"
                     /old_locus_tag="MUA31_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002499696.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            12372..12560
                     /locus_tag="MUA31_RS13675"
                     /old_locus_tag="MUA31_13670"
     CDS             12372..12560
                     /locus_tag="MUA31_RS13675"
                     /old_locus_tag="MUA31_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_105977915.1"
                     /translation="MDKINYFLKALVLAIMLRCTMNYLFPTPEKLSFKLLDGLLFGVLV
                     CLLIIWFIDIFKKSSRK"
                     /name="hypothetical protein"
     gene            12835..13371
                     /locus_tag="MUA31_RS13680"
                     /old_locus_tag="MUA31_13675"
     CDS             12835..13371
                     /locus_tag="MUA31_RS13680"
                     /old_locus_tag="MUA31_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_105977884.1"
                     /translation="MINILQAVGSIGTFIMAILYLISVMIQIRQIKISFIPFLAFDQII
                     MEQEGKSLKLRNLTETDSDYVNTAFKLQNLGGGTAKNIDIKVHLNENAVIQEKHINILP
                     SDKYYLMPIGKKAFEEFQDTVKNNGYTTNMYINISYYSQISKKQNNTRYRVNIEEFVNL
                     DDKDLYELTFDTIEA"
                     /name="hypothetical protein"
     Typing          10321..11353
                     /name="rep_cluster_910"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.093"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agttcaatat ctttcttatt gttaatgaat ttgacaataa gaaaagttgt taactctcct
       61 tagttttaga gttaacgact aaagcgaaag ttactgatat taaacattaa tttagatact
      121 tgtttgtgta tatcttgtaa atttgtgttt ttaatatttt cggacgtgtc tttttgacac
      181 ttccttgtct tgcaaggttt ttgatgtttt tttcttctga attggaataa agtaattatg
      241 ttgttgtgat ttaaaaaaag gaggtcttgt atgagattcg gaaattataa aattgattct
      301 ttttggttaa ttatgattat aggtttctta gctacttcta tattttttcc ttttatgctt
      361 ttgtctgtag ttcttttatt gatttttggt ttagaaaaaa atgataaaaa ggattaaatg
      421 tgagtttttg gttttggttt ttgaaacgag tttcttcttt tcttgatata gaacagaaat
      481 tatccaataa ttgttcatca tttccttttc taaaaaaaga accactctga tatgccaatt
      541 gatactcatc ctttatttat tactaatata tcatagtatt agaaacacgt ataaaggaac
      601 ttttatcttt tttaacataa gatcacttag cggaagttaa ctattatcat atattcgaag
      661 atgatataat atataagtcg cctatctctc aggcgtcaat ttagacgcag agaggaggtg
      721 catatcagat ggtcgaagct cttgtacaca tcacgaccac agtcatcagt ggttgtattg
      781 ttgcgttatt tacgcattgg ctacgtacac gtaataataa gaaaaaatag gcgatattag
      841 ccaacaccta aaaataccct cactactcgc aatagtgagg gtattggtgc atataatgta
      901 cgaagctctt gttaattaaa ttataacact aattagtaga agaatgcaaa attagtatct
      961 atatggtaaa tagagttttt attaatgctc attatattta agcgagtaat gtcttttagt
     1021 taatataaaa gtcaacaagt ctatataaga tttgtcgaca atctggaatg catttacggt
     1081 ctacctaaat aatacaatta cttttaacca taccttatag atactttagt taactagcac
     1141 atgtgcaata aacacttgtg ttaagtgtac atgtgttgca cttctttttt aactacttat
     1201 tatcctagta tttcatcatt ttttctgtaa gaacgagttt atattttaat tcagctttac
     1261 gcattttcat tttttctaat tctttttttc tcttcattga ttaaaccaac cttaacattt
     1321 attattgata tcaagacacg aagaaactcg ttttcaactc gttcaagaac ataaaacaag
     1381 gagaaaaatg tcatatgata attacaattt tattattggt attaatcgtc aatttactag
     1441 aaagtctgta tctaggcata aaatacttga gattaaagaa acaaaatgca gcagataaag
     1501 aatatacaaa aatggtggaa aaagtagccc cattaatgta cgtgactctc gttatctcag
     1561 tgattgcatt agttatatca tggattatca gctaattaaa aaagataagg atattattac
     1621 tttattaaca ataagaaaaa aagaaactag tttctaaaac ctaaaaaaca ctagccataa
     1681 taatcaaagc tattgcgcaa ataatggcga atccagcaaa ataaagactc tttttataat
     1741 cacctttttt cttgcttttg cttataactt tataaacttc agacccgcaa atcactaacg
     1801 ctgaaattcc aaatacaata aaacctatcc caaatacata acctaaaata tctaaaagca
     1861 taacttctaa cctccataaa ccttatttac actaaggtta acaaagaaaa gaaggggcaa
     1921 aactaacaat attgttatac tcaagattgc tttattgacg ttgagccgcg gaacccttaa
     1981 caatcccaaa acttgtcgaa tggtcggctt aatagctcac gctatgccga cattcgtctt
     2041 caagtttagt taagggttct tctcaacgat caataaattt tctcagcata aatgcgtcat
     2101 ttaaaactag ttagtcgtct atacttaatt taaaaatttc ttctttcttc tcagttaatt
     2161 ctttaagtat aatcttcggc tttttttcag aaagcaatat gtcaaaagcc tcattataga
     2221 tagctttcgc ttctttagat ttagacgtag aaactctact ataaatattt cctatctctt
     2281 caagtaaact cgcacttttt ttataataaa ctaaaccgtc tttattcgct tcaagttcaa
     2341 caaaaccatt tctaatccca tattttaaag cataataaag taaaatagcc gaaaatatta
     2401 tatatataaa tatcaaatta atacctcctt ttttaacaaa ctttatcaca agaactattt
     2461 aaaattttaa acgcctttat tttggatttt aaggggggat ttaaagcaaa gtctgtgttt
     2521 cttatatgtt tgttactcaa gtcccaatat aagcatttaa aacgtaaata tgggcttata
     2581 acagaaaaat atccctactt gatttataca taaaatatac accgtcaaaa acttaggata
     2641 tttgcgtagg atattttaca tgagcataag gaaatccagt accacccctg gtggtattca
     2701 gcatagctga aagattttcc taacattcgt tacactcatt tgctcatgca aacaccacca
     2761 aaaccttgat attaaagtaa ttgaagtaaa tccgtatttt ttagcctatg aaacatcaaa
     2821 gtgtattagt acatagaatg caatatgtta cacaaatacc actcagagct tcccatagga
     2881 tatttttttc attgatttga cggcttttat tcagggtcta ggatacgaat atcctagatt
     2941 ttaacaactc taggatatat aggatattca ttttttcgta ttttttgtat gaaacgctta
     3001 aatatctaat ttaaaaccaa cataaaaaac gctagctttt tcgctaacgt tttaaaaaga
     3061 atatcaaaat ttataatcaa atttcttcgt cgatttcata tatctccata atgcatgagt
     3121 tgttaaaact agtcccatag aaaaaaagaa aacaataata atatttaata ctatacttaa
     3181 gaaattatag gttggtaagt atataaataa aaggaacatc gggatagtaa acaataaatc
     3241 ccaccatcct aaagataata aagatttttt tagattttcc ataagatcac ctatttttta
     3301 tgagagtact ttacatgctg catctttccc gccgaatttt tcaacagctg tttggaaaat
     3361 gtatctacaa gcagctcctc cagcgaatcc agtagcagca ccaaatggtc cgctaaccat
     3421 agcaccagct gcaccagctc ctaatgacac gccaatgcaa gctgaataac tagagaaaat
     3481 acatgcccac ttaagtgcag ctttatttat agcatatgtt gtaacaccat tatctacagc
     3541 tgtactttgc aacagaaccc atttatctat agttataatt atttaacatt ttcctcaaga
     3601 taactccaaa gataaatcct ataatagata gaggaatcca tcccattcct attgaggata
     3661 aaggtatata tttctcaaac actgatgtta tactctcaaa taaatttgtg tcagaaatga
     3721 aaggtggaca atgtttaata gcatctatga ctgcagctaa aagagtgaat aatatagtag
     3781 agttatacac aatcttagaa caaaagaatt taaaatttat aatttctaat aaaattaata
     3841 ctattgctaa aggatatagc aatagtaata taggagttga aattttaatg atgtatgtta
     3901 aacccgtatt cgatattaag aaagaaacaa tacaagataa agtagtaaag aaaatataat
     3961 taaatttagg gaataagtta ctaaatgttt ctgaaaaaga agcgtttagt ccaatagctg
     4021 tttttaagca agctatagtg ataatgactg ctagaaatat catgccatgt tcccctatgt
     4081 agtatttaat aataagagtc aatgtgattc cgccattttc atgtggttca aactctccta
     4141 agctcattga tccgataata gctagtaaac tatatattaa tgccatgagt accatactaa
     4201 tagctccaga tttaattgtt tctctagtta tatttaatgg ttctgagatc cccatatctt
     4261 ttatggtttt tacaataatc atcccaaaag ccaatgctgc taatgcatct aaagtattat
     4321 agccgtctac aaaaccttta aaaaaaggag tttgtttata ttcattagaa atagagccgc
     4381 tagtgattgt acccataggt tgtaaaaagg acaaagttaa tataatactt aaaagaaata
     4441 caaaaatagg attaagtatt ttccctatgt attccaatat ttttgcagta tttcttgaga
     4501 acacccaagt taatatgaaa aataataaac tgaatataaa aagaaatggc ttgtataagc
     4561 tttgactaat aaaaggagat aacccaactt caaatgatgt agatgccaac ctaggtaaag
     4621 catatattgg accgatcact aagtataatg ttatagtaaa gatatatgca tagtgtctat
     4681 taactttcat tgatattgca aaaatatcct ttttttgtga aaaaccagct gctattactc
     4741 ctaaaaacgg taatcctatt gcagtaacta aaaagcctat attagctaat atgacgtttg
     4801 ggccggaggc ttgtcctaaa tgaataggaa aaatcaaatt tcctgctcca aaaaataatc
     4861 caaataacat agagccgata tacatatttt ctttccatgt taatttattg tgcataaaaa
     4921 tcccctcctt tattatatct ttgaaaccta taatatatta ataagcatca atgagaaagt
     4981 ttataatcct aagtcttcta agataataaa gattagttct ttatagaaat tattatgaaa
     5041 tgatgtcatt tatagttgaa gttgtcaaga aagcttttgg gagatttctt ttcttgccat
     5101 tttgttacgg tatcaccttc caaatggcat aaaaacttat catctactaa tatatactct
     5161 ccatcttctg atccatgacc taaaacttcc acattatgat tcttgttctc aaaatatcta
     5221 attaagtaat ttttcatatc ttctaatgat ttaatataat cttctaattc atcattatta
     5281 ataacatagg aacctacttt actttgtcca gaaattattc cttcaatttc atctactata
     5341 tttaacgctt cccaagttat tgatatatcc ccctcttctg gagaaaacat atatttacta
     5401 agtatgaact tgctgccttt ccaattaatt tctgagtatg aaggaatttc catgtattct
     5461 aaaaaatagt ctgtgataac ccttccatca attttttcct tataaatttt tatattttct
     5521 atagaactta tccatgactt aacatcatct tctgtcttaa ataatcccac catttttcta
     5581 gttgaaaatt gcttgaattc tagtaaaaac atctcgtatt ctcctcttaa tatgtataac
     5641 ttatggtata aatatatcga tagtgttttt aacgaaagta tatctcccta tagtttaagt
     5701 aaaaaattac tgtttatcca cttctttttt tgtgataaac agtaattttt acttatcgat
     5761 agaaaaatga taactatttt caaaactata ttttttatta cgtataaatt ttctggttct
     5821 gctgcaaagt tgattttata gcaaacttta acaaaaatat tctactcaga cacttaagca
     5881 tgcttttacg tctggtataa atacattatt taggttaaat aatcacttag gaacttcaaa
     5941 tgattattta gggaatttta aactataact cgaaaaaatt tgtataataa agttaacgat
     6001 agcgaaaggg gatgaagttt atgatggttg ctgagattat cggcggaatt attaaactta
     6061 ttaagacact cgttgacact tttgcttaac ttaaaattga atttctaata caagaggagg
     6121 aaatatttta tgtcaggtat tgtagatgct atcagtcaag cagttcaatc aggtctagag
     6181 caagattggg taacaatggg aactaatatc gctaaagcat tagcacaagg tattgatgtt
     6241 attgctggtt tctttggcta atataaataa ttaaagtagc cccaactttt taaagagttg
     6301 gggctacttt gattatttat gatgaaaaat caaaaaagat ggttaagcag tactttaaca
     6361 tgttggtaaa gtacttgtag caaatatatt gttgaagatt gatagataaa cagataggat
     6421 tcctctcaat caaagagtac actaaaaaat caacttcgct agttcggtct gtatacgatt
     6481 aatcaggcgt tcatctaaag tagatattat agttaaaaat ctaaatattt ttaattgatg
     6541 ataatatatt gatgctttaa ttattttatt tttgcgttaa gtaaccatct tccatatgaa
     6601 agattttgtc gcaaaattta gttaaccgtt catcgtgagt aacaataata catatttttt
     6661 gacgttgttt agactgttcc tttagtattt tcataactgc catcgcattt tcagtatcga
     6721 gtgatgcggt tggctcatca gctaaaataa tagatggctg tgtataaatt gctttagcaa
     6781 tcgcaacgcg ttgtttctgt cctcctgaca cttcagaagg taatttattc tcgatttctt
     6841 tcaaacctaa atcgtctaac aatttatcat gttcttgagt acttaacaca tcttttttat
     6901 aactctttaa taagtgaaat tgttgtttga ttgttaagaa tggaactaag ttagtcgctt
     6961 gtaatataaa tccaatttct ttcattctca ttctggataa ttttttctga ttcatttttg
     7021 agattttctt atcgttaata taaatttctc cagatgtagg tgtttgtaag gcaccagcca
     7081 ttgttaggaa tgtactttta ccagaaccag aaggaccaac aatagctata aaatcgcctt
     7141 tttcaaaaga taatgatgtt ggtttaactg ctttaatcat atgatttcca tcttgaaatt
     7201 cttttgtcac tttctcgaat tttaacataa tgaaccctcc ttatccgatt gccttaagtg
     7261 gatcaatttt tctaatagat agtactgaga aaagactacc cacaagtgaa ataagtatca
     7321 gaacaatgcc gaaaataata agtgttataa catcaaattg aatcggtact gctttgggta
     7381 aaattagact tgtaagcagg gtcaacatta atccaattaa tgagccgatt aaagataata
     7441 taaaggtttg tgctaacacc atcttcatta agaatccatc cgtaaatcct tgcgctttta
     7501 atacaccgaa taaactcttt ttttgtagag taataacata taagaatact ccaataactg
     7561 tagcagaaat aacaaacaaa aatgtaatca tgaaattcat tgttaaattt tgagatttat
     7621 aacctggtag attttctaca aattcatcaa taccaatcac ttctaaatca ctatttatct
     7681 ttttatcatt ccaattatta tctttgacaa ctactgaatt ggttttgtct gacgataatg
     7741 ctgggttaat tttttccacc gttttattat tagtaaaaat aacaggagaa gcattatatt
     7801 tcgcactttc tgacactcca acgatatgta atttctcgtc agattgagat aaattgatta
     7861 tgtcattaac tttaaatcct ttatctttca atgattgatc aatgacaacc tcattatctt
     7921 tattaaacgc tttgccttcc ttgatcttcg gaatcaagaa tgaatttgat gttaccccaa
     7981 agagtatcgc attctcttta ctatttccct ttgaagcgat aatgcccatt tgctttaaag
     8041 gagcctcttt tttaaatttc cctttaacat ccgatgttgt tattactgat tgttgtaccg
     8101 tttgattagc atctttattt aagacaattg catctgcttg ccacttctta ataccttctg
     8161 tattcatatt aattaaacca ttcgctaatc cagataataa aaaaagtaag taactaatta
     8221 atacaagaat gcctataatt aatccaaatt tcagcttatt gtatttaatt tcgttccatg
     8281 ccaaaaacat ttaatcctct cctttaaaca cgatatcaat aagtataaca ataaaagctt
     8341 ttatatatat attagaatca catttttgta tgattatggt acaccctaat catacaaatg
     8401 cggttattga aattacaata actagaacgt atgattaggg tgtatattaa ttatgaggtg
     8461 gaaatcaatg attgttggtt atgcgagagt atcatctata gaccaaaatt tagaaagaca
     8521 gctagctaat ttgaaaacat ttggcgtaga gaaaatattt acagagaaac aatcgggaaa
     8581 gtcagtagaa aacagaccta tatttcaaga agcacttaac tttgtgagaa tgggcgatag
     8641 atttgtagta gaatcaattg atcgcttagg tcgtaattat gatgaagtga ttaacacagt
     8701 taattattta aaagataaag aagtccaatt gatgattact agtttaccta tgatgaatga
     8761 agtcattggc aatcctttat taaataaatt tatgaaagac ttaatcatcc aaatattagc
     8821 aatggtttca gaacaagaac gaaatgaaag taaacgtaga caagcacaag gtattaaagt
     8881 tgcgaaagaa aaaggggtat ataaaggacg tcctttactc tatgcaccga atgctaaaga
     8941 tcctcaaaaa cgtgttattt atcatagagt tgtagaaatg ttagaagaag gccaagcaat
     9001 tagtaaaatt gcgaaagaag taaatataac aaggcagaca atttacagaa ttaaaaaagg
     9061 taatgatttg gtttaataaa ccttgtttca acgcaaatga gatcatctca tagcatccta
     9121 gaaatcacta taatatttta ttaataaaat aaaaagagag caaacacgct ctcttttttt
     9181 tactttacat aatcgattta tcagaagtgc caataataaa aataatatcc ttacaatctt
     9241 ataaacttgt aaggatattt actgtgttaa ttaccaaaca atcggctcca gaaacctttt
     9301 ttaggtggtt tttcttccct ttcttcgtat tcactatcta tttcttcgaa ttgtttatcg
     9361 tctattgtgg atggttcttc gttaataatt tcatcctctt tctcttcagg tatatcttta
     9421 tgctgcgcct ctgtaggttg ttcccattct tgttgttgat cagtatcttt agactctcta
     9481 gtaaatgttg cttcttgtac attaacattt tgtctctctt tagcagacgt atcaaaagaa
     9541 taattaagtt gtttttcttc ttctaactca gactccaacc tttgtatttt tttgttactt
     9601 tctaatgtta atacttgttg gttttcaagt aattttgacc tttcttgcaa ttctttccta
     9661 taaaattcta ttgtcacttc atgacgcttt tcttgttttt caagttgttt atttaaagtg
     9721 tctatttgct tttcaagcag ttcaattatt tgaacattat gtttagattc atttttttga
     9781 ttactcgttt taactttttc tgagtttgta ttagtacttt caaacccata tttttttttg
     9841 tttttggtta ctctttgaat aatcttttct acatcaacat cattttttat gaatgaagta
     9901 ttatcctttt ttattgtttc tatattcagt ctttttatat tattaaatac agtttgttta
     9961 ctaacttcta atttttcact gagttctttg acactcttca tattatcaac cctttataaa
    10021 tcctttatca accctttatc aaccctttat aaatcaattt tatcataaat gtttttataa
    10081 cggtgatttt aagaattcta aaaacccttt acaaacactt gacgatattt ccatcaagtg
    10141 tttgtaaagg gtttttagtg cgcctataat atcttttata tgttctcttt taatgatata
    10201 atattcataa tacgttttag tcccaactac tggtaatagt tgggactggg taataaaaac
    10261 gttacaatac gtcagctatt taatttgata tatgtattat atataacaat atcgacagtt
    10321 tgcaagccaa aaagttatgt ggatttgtaa tgttttatta cctctattca aatttaattg
    10381 gaaagaggtt tttattgtgt ctcgtaaaaa tattaaaaat caagctagtc aaaattttta
    10441 tatgctacat aaagcattat ttgttaacga aaaatataaa aaattaagtg atagcgctaa
    10501 agtcacttat gcaattctca atgatagagt gagcttatcc attaaaaata attggatcga
    10561 tgagaacggg gatatatatt ttatttttac aaatgaaagt ctccaaaata tattagacaa
    10621 aagtaaaaat acaattacta aaataaaaaa agaactgcaa gaagtagggt tgcttgaaca
    10681 aattcgtaca ggctttaaca agccgaacaa attatatttg catgatatag aaactaatat
    10741 tggtgtggaa aaaaatattc aaaactcctc tgagtcttat gatatcaagg agtcccaaat
    10801 tttgggactc cagaatcccg aaatttggga ctccagaatc ccgaaatttg ggactccaga
    10861 atcccaaatt ttagacccta atgatactga ttatattaag actgattata ttaagactga
    10921 gaataatgat acggatgatt tgcatgataa aaaattaaca tatcataatg atcatacaca
    10981 tcattcaaat cactacaatt caaactttaa tgatgaagct ttaaaattcc aattactcga
    11041 agaattaccg caaaacattc aaaattattt aaacaatttt tctgtacttg aaattaagat
    11101 tattaagtct gtactcttaa aagccaagac ttcttttaac aattcgattg attcatatta
    11161 cttattggaa gatatggagt tagaagtgat ttatgtactt aaacgtttta aagctatgct
    11221 tattcaaaaa aatgaaagcg tccaagcaat gcaaggttat cttatgacat ctctcaaatc
    11281 tgaattcgca gagatgcata cacttaataa acgacgtgag catttaccaa ttacttcttt
    11341 atttaatcaa taacttactt taattactgt taacaaatcg tattttacag ataaacggta
    11401 ttatattagt caaaaagaat ggctgaattt atggcaagat gtaacgggta acgatgaaat
    11461 tacacaagta catgttcaaa aaataaaaca aaataacaac aaagaattat acgaaatagc
    11521 gaagtattct ggtaaagata gcgactactt aattaatcaa aaagtgttcg atacatttta
    11581 taaatcactc aaagggaaac aaattcttgt ttattctgga ctgtttaaag aggcaagaaa
    11641 gaaattgaaa aatggagatt tagattatct taaagaagta gatccgacag aatatattta
    11701 tcaaattttt tatcattgga accaaaaaga gtacttagcg agtgaaattt ttgatttaac
    11761 cgaagaagaa aaaagtaaga ttaatcatca aatgattgat gaaattgacg aagaaatttg
    11821 agtataaatt ttgatatttt ttctaagacg ttagcgaaaa agctagcgtt tttttattga
    11881 aaagtgctaa aaaatgtttg ggtacatttt gggtacaaaa gtaaaaaaat gattgcactt
    11941 tagaaatcgc tgtatccctt gaaatgtctg gcttcgccag accaaccttt tttgaaaggt
    12001 tgcaaatttc ccttatgctc ttacaaagca ttttgggcaa aatcaagatt tcggtatttt
    12061 ttacagaaat ttgcccctgc aggaactaaa aacccaactc actttttgga ggattatatg
    12121 caaaatttta gtttgcatgt aactgggcag tgtctaaaaa tcgtcactgg ttttgctaaa
    12181 aatttgtgat gtgaaatcaa aatatattta tttggctctc atttgcgttt taagcgctta
    12241 tatagaattt cggatataaa actacgtaaa tatttagtgt tcttcaaaat gcccctttaa
    12301 aattaaaaat aaaggcactc aaaatttttg taagagttat gataaagttt gcttaaaaag
    12361 gagtggaata ggtggataag attaattact ttttaaaagc attagtatta gcaattatgc
    12421 ttcgttgtac aatgaattat ttatttccaa cacctgaaaa attatctttc aaacttttag
    12481 atggacttct ttttggtgtg ttagtgtgtc ttttaattat ttggtttatt gatattttta
    12541 agaaatcttc acgaaagtag aacacgcatt tatgccgaga aaatttattg atcaatgaga
    12601 agaaccctta actaaacttg aagacgaatg tcggcatagc gtgagctatt aagccgacca
    12661 ttcgacaagt tttgggtttg ttaagggttc agaggctcgt tgtcaataaa gcaattggaa
    12721 taaaacattt aaatttaatc tgtttattat tggttatcaa atattattaa tgtattaaca
    12781 atacattgca ttatgataga atcaaaagac tacttatttc aaaaggagaa ttggatgata
    12841 aatattttac aagcagttgg ttcaattgga acatttatca tggcaatttt atatcttatt
    12901 tcggtaatga tacaaattag acaaattaaa atttctttta tcccattctt ggctttcgat
    12961 caaattatta tggaacaaga aggaaaaagt ttaaaattaa gaaatttaac tgaaactgat
    13021 tctgattatg taaatactgc ttttaaacta caaaatttag gcggtggaac tgctaaaaat
    13081 atagatataa aagtccattt aaatgaaaac gcagttatac aagaaaaaca cattaatatt
    13141 ttacctagtg ataaatatta tttaatgccc attggtaaaa aagcgtttga agaatttcaa
    13201 gatacagtta aaaataacgg ttatacaacg aatatgtata ttaatataag ctactatagt
    13261 caaatctcta aaaagcaaaa taatactaga taccgagtaa atattgaaga attcgttaat
    13321 ttggatgata aagatttata tgaactaaca tttgatacta ttgaggctta aatacgtaaa
    13381 taaaaaactg cggttttatt atcagaagtt ccgtcaatgt aaaaggcgta tcgagggttt
    13441 ataaaccctt gatacgcctt ttatattcat tttttgatta acatctaatt taacatcttg
    13501 ttgtgattca ttattttgtt gtttaatcta ctttgtttat ttttatttgt agtagtcttg
    13561 ttctttctta gtattattag ttctctctgt atgtgaatct ggattccctt tgtgtgtgaa
    13621 tccttgatat gactgagttc aatatctttc ttattgttaa tgaatttgac aataagaaaa
    13681 gttgttaact ctccttagtt ttagagttaa cgactaaagc
//
