LOCUS       NZ_CP094700             4447 bp    DNA     circular CON 20-OCT-2023
DEFINITION  Staphylococcus simulans strain IVB6241 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP094700
VERSION     NZ_CP094700.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26923386
            Assembly: GCF_025558185.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus simulans
  ORGANISM  Staphylococcus simulans
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Akarsu,H., Liljander,A., Younan,M., Brodard,I., Glucks,I.,
            Labroussaa,F., Overesch,G., Kuhnert,P., Perreten,V., Drexler,J.F.,
            Corman,V.M., Falquet,L. and Jores,J.
  TITLE     Comparative Genomics of East African Camel-Associated
            Staphylococcaceae spp.: Diversity and Inheritance of Traits Involved
            in Host-Pathogen Interactions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Akarsu,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2022) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 901x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/20/2023 05:20:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,729
            CDSs (total)                      :: 2,649
            Genes (coding)                    :: 2,615
            CDSs (with protein)               :: 2,615
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 15 of 35
            Pseudo Genes (incomplete)         :: 17 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 2 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP094700.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Staphylococcus simulans"
                     /mol_type="genomic DNA"
                     /strain="IVB6241"
                     /isolation_source="Pus swab"
                     /host="Camel"
                     /db_xref="taxon:1286"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Laikipia"
                     /collected_by="Joerg Jores, Anne Liljander, Mario Younan,
                     Jan Felix Drexler, Victor Max Corman, Ilona Glucks"
     gene            complement(join(4430..4447,1..372))
                     /gene="mobC"
                     /locus_tag="MUA31_RS13570"
                     /old_locus_tag="MUA31_13565"
     CDS             complement(join(4430..4447,1..372))
                     /gene="mobC"
                     /locus_tag="MUA31_RS13570"
                     /old_locus_tag="MUA31_13565"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_262547469.1"
                     /translation="MNEHKDNLASDLSVGGNRKPNRKEPKQISFRVSEQEYSKLKQSAE
                     TLNMTVPNFVKKKAQGSRLVSPKLDKETRQSIAKDLSHLGSNVNQIAKWFNQNKDKATH
                     LPEEKYHDLEKQFDDVKKELVVFFL"
                     /name="mobC"
     gene            complement(642..1490)
                     /gene="str"
                     /locus_tag="MUA31_RS13575"
                     /old_locus_tag="MUA31_13570"
     ARG             complement(646..1490)
                     /name="str"
                     /gene="str"
                     /gene_name="streptomycin adenylyltransferase Str"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(1498..2442)
                     /locus_tag="MUA31_RS13580"
                     /old_locus_tag="MUA31_13575"
     CDS             complement(1498..2442)
                     /locus_tag="MUA31_RS13580"
                     /old_locus_tag="MUA31_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011167206.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_011167206.1"
                     /translation="MSKKAEEIQAKQSLEKENSNFSKTGYSNSRLNRHIMYTPEPKLHF
                     DAMTIVGNLNKNNAHKLSEFMSIAPQIRLWDILQTKFKAKALQEKVYIEYDKVKADAWD
                     RRNMRVEFNPNKLTHEEMLWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKSVK
                     KTIFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADIEVMSEHLWRVEIELKRDMV
                     DYWNDCFNDLHILKPDWSSLEKVKDQAMIYMLIHEESTWGKLERRTKNKYREMLKSISE
                     IDLTDLMKLTLKENEKQLQKQIEFWQREFRFWE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(3003..3458)
                     /locus_tag="MUA31_RS13585"
                     /old_locus_tag="MUA31_13580"
     CDS             complement(3003..3458)
                     /locus_tag="MUA31_RS13585"
                     /old_locus_tag="MUA31_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="WP_000753824.1"
                     /translation="MKKTTEDFQGRGKKVHNDFVRLLQKNLNRVNAEDIEIEVRKDVYG
                     VREEIHSMLREVKQSHEHYQKRQKQLFTGIGAMLLVFMLFALIMTIGRDFMGFLHVDVL
                     QNAIASKIKASEGFISVLWYIAYGLPYILAIGLFIWLYEWIRARFHD"
                     /name="DUF334 domain-containing protein"
     gene            complement(3427..4389)
                     /locus_tag="MUA31_RS13590"
                     /old_locus_tag="MUA31_13585"
     CDS             complement(3427..4389)
                     /locus_tag="MUA31_RS13590"
                     /old_locus_tag="MUA31_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011167204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000246202.1"
                     /translation="MATTKLGNTKSASRAINYAEERAEEKSGLNCDVDYAKSYFKQTRA
                     LYGKENGVQAHTVIQSFKPGEVTAKECNEIGLELAKKIAPDYQVAVYTHTDKDHYHNHI
                     IINSVNLETGNKYQSNKEQRDFIKKANDQLCEERGLSVPEKSSEIRYTLAEQNMIDKDK
                     RSWKNDIRMAVEETKDNAVDFEEFNTLLKEKGVEITRVTKNNVTYRHIEEDKKVRGNKL
                     GDSYDKGVIENGFAIEKFRREREEEREYDEYADTFEVDWDAVAENSEDLRKRRIARTEE
                     TKQASNKIYIRDERTTGLERKGIAGNQVEFEKDDGGLSR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          344..578
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="80.0"
                     /coverage="100.0"
                     /evalue="1.4099999999999999e-40"
     Typing          complement(1499..2442)
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3431..4389)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caactccttt ttcacatcat caaattgctt ttctaaatca tgatatttct cttctggtaa
       61 atgtgtagcc ttatctttat tttgattaaa ccacttagca atctgattca cattactacc
      121 caaatgactt aaatctttgg caatcgattg tcgcgtttct ttgtctaatt ttggcgacac
      181 taatcgactg ccttgtgcct ttttcttaac gaaattaggc actgtcatat tcaaagtttc
      241 tgccgactgt ttcaactttg aatattcctg ttcactcact ctgaaactaa tttgttttgg
      301 ctctttgcgg ttgggtttac ggtttccccc aacagacaaa tcgctagcca aattatcttt
      361 atgttcgttc acaagtccca ccacctttct gacgaaaaat attccttttc ataaatatag
      421 cttataatat aatctatatt ttctcaagta atatagtggt ttggcaagcg tcgtatattc
      481 tagccacttc gttctgaata taccggcttg ctgggattaa tcccagaccc tttatgtttt
      541 tgctaacgca aaaaatgagt ggcaaaatgc tagccactca tagttcaaaa ccaaattcaa
      601 aatcaaaaca aaagcaacca aaaatgtggt tgctaataaa attaattctc ataattatta
      661 cgaataaaat tctcaatatt tttttcgtaa tttggatagt tataatttcc taaacaactt
      721 actttatttg aataatattt aaataattca caacaaagct taaaagattg gtatgtcttt
      781 ctatatccat tcatctcaaa agtagctaaa agcatattga attctttatc agttaaatat
      841 ttgtttataa acttataatt ctttcctaaa ctaaaatcaa aacccctatt aaagccaata
      901 taccaagaaa tcattcttaa taattcagga cgtaaaatat tattgaaatg atctaaagca
      961 aataatattt ctcttctaaa aacacccttt gctacatacg ttgagacact ccaaaactca
     1021 ttacagcaat cataaaattc tcgttctgtt ggttttttta accaataatt tgagtcatct
     1081 ggaacaattt cttgagttac taaattatct ttatcaacta aaatttttac aagaccatca
     1141 gaatcactaa aataacgatt taaatctttt aaattaatta atgtaatatc catttttatg
     1201 ccatctttaa aatacattat ataactgtaa ccataatcta aatcaggtgg gaataattcc
     1261 atatcttctg gtttctgtat aaacaataat tctccaaata aacttaacca actttcttca
     1321 tgtgtgaaat actcaatatc tgatacgaaa aaagcaaaat cataatcttg aaacttatct
     1381 ttttttatat tttcattagt tcttgaaccc tcgagagcaa taatttttac attgcttcgt
     1441 tgataagcaa attcagaaac taaatttaaa atttcttttt cagtcctcat tgttttttca
     1501 ctcccaaaat ctaaattcac gctgccaaaa ttcaatctgc ttttgcaatt gtttttcatt
     1561 ctcttttaaa gtcaatttca ttaagtccgt taaatcaatt tctgaaatac tttttaacat
     1621 ttctctgtat ttattttttg tacgtctttc taatttaccc catgtacttt cttcatgaat
     1681 tagcatataa atcattgctt ggtcttttac tttttctaaa ctagaccaat ctggtttcaa
     1741 aatatgtaaa tcattaaaac aatcgttcca ataatcaacc atatctcttt ttaattcaat
     1801 ttctacacgc cataagtgtt cagacataac ttcaatgtct gcattatcct tacgttcttg
     1861 ttttttatta taaattctaa taaatctatc actgtcacga acaccaaaat attttgtttc
     1921 tggtttaccg ttacgaccat aaaaaatagt tttcttaact gatttatcag tcatcgcata
     1981 ataatcgctc aaatcgtctt caaaatcaaa agccaaatct aatcttgtaa aaccatcatc
     2041 ttccatgtag tcaatgatgt tttgttttaa ccaaagcatt tcttcatgcg taagtttatt
     2101 aggattaaat tcaacacgca tattacgtct atcccacgca tctgctttta ctttatcata
     2161 ttcgatgtaa actttttctt gtagagcctt agctttaaat ttggtttgta atatatccca
     2221 aagtctaatt tgtggcgcaa tactcataaa ttcagacagt ttgtgagcat tgttcttatt
     2281 aagattccca acaattgtca tagcatcaaa gtgtaatttt ggttcggggg tgtacataat
     2341 atgtcggttt aaccggctat tagagtatcc ggttttagaa aaatttgagt tttccttttc
     2401 caaactttgt tttgcctgaa tttcttctgc ttttttactc ataaaaaatc gactccttat
     2461 tttgtttaag aagtcgctca cccgaatata tatcttgaag aataaaccaa tatcgtttaa
     2521 tatctagata tacaaaaatt aagaaaaaaa caccaactgt ttttctttaa ggtaagtcgc
     2581 attcacaaac tttggtcagg gagtgagcga cttctttttt tgttttttta tttgcatatt
     2641 aatataacac taacaaaagg cttggtctat tcagactaag tcttttttta ttaggctgaa
     2701 tattttttag aatatttata ttgctgataa gtgttataaa gtattaacgc ctgcactact
     2761 acttgagcaa ttgatattgc taaattaatc tttttcttct tactcatttt cttcaacccc
     2821 ctttgttgct ttattccaat tgctttattt acattgagcc tctgtaccct taacaaaccc
     2881 aaaacttgtc gaatggtcgg gctaatagct cacgctatgc cgacattcgt ctgcaagttt
     2941 agttaagggt acttctcaat ataaataaat tttctcggca taaaagcctg atcatacttt
     3001 gtttaatcat ggaatcttgc tcttatccac tcatacaacc aaataaatag ccctatagcg
     3061 agtatataag gtaaaccata agcaatgtac cataaaactg aaataaagcc ctcagaagcc
     3121 tttattttgc ttgctatggc attctgtaat acatctacat gtaaaaagcc cataaaatct
     3181 ctgccaatcg tcataatcaa agcaaaaagc ataaacacta ataacattgc accaatacca
     3241 gtaaataatt gcttttgtct tttttgataa tgttcatgtg attgtttaac ttcacgcaac
     3301 attgaatgaa tttcttctcg aactccataa acatcttttc gaacctctat ctcaatatct
     3361 tctgcattca ctcgatttaa attcttttgt aacaaacgaa caaaatcatt atggactttt
     3421 ttacctctac cttgaaagtc ctccgtcgtc tttttcaaac tcgacttgat tgcctgcaat
     3481 tccttttctc tcaagtcctg ttgttctttc atctctgata tatattttat tactagcttg
     3541 ttttgtttct tcagttcttg caattcttcg ttttctaagg tcttctgaat tttctgcgac
     3601 tgcgtcccag tcaacttcga acgtgtctgc atattcatcg tattctcgtt cttcttctcg
     3661 ttctcgtctg aatttctcga ttgcaaagcc attttcaatc actcctttgt cataagaatc
     3721 gcccaactta ttcccacgta cttttttatc ttcttcaatg tgtctatacg ttacgttgtt
     3781 tttcgttact ctcgtgattt caacaccttt ttcttttagc aaagtattaa attcttcaaa
     3841 gtctactgcg ttatcctttg tttcttcaac tgccattctt atatcatttt tccatgacct
     3901 tttatcttta tctatcatgt tctgttctgc caacgtatat ctgatttctg atgacttttc
     3961 tggcacagat aaaccacgtt cttcacataa ctgatcattc gcctttttta taaaatcacg
     4021 ttgttctttg tttgactgat acttgttgcc tgtttctaaa tttactgaat taataataat
     4081 gtgattgtga taatggtctt tgtctgtatg tgtataaact gcaacttgat aatctggtgc
     4141 aattttttta gctaattcta aacctatctc attacactct ttagctgtta cttcgccagg
     4201 cttaaaagat tgaatgactg tatgtgcttg aacaccattc tctttgccat ataacgccct
     4261 agtttgctta aaatatgatt tagcataatc aacatcacaa tttaagccgc ttttttcttc
     4321 tgcacgttct tctgcgtaat taatggcacg acttgccgac ttcgtattgc ctaatttagt
     4381 tgttgccata tcttatgcaa ctcctttaat aaaaaaaaaa aaaatcctat tataagaaaa
     4441 aaaccac
//
