LOCUS       NZ_CP094235             2426 bp    DNA     circular CON 11-APR-2024
DEFINITION  Enterococcus faecium strain NY13320 plasmid pNY13320-5, complete
            sequence.
ACCESSION   NZ_CP094235
VERSION     NZ_CP094235.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26755017
            Assembly: GCF_022749455.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of strain NY13320
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2022) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.
            20, Dongdajie, Fengtai, Beijing, Beijing 100071, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022749455.1-RS_2024_04_11
            Annotation Date                   :: 04/11/2024 17:08:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 70 of 145
            Pseudo Genes (incomplete)         :: 66 of 145
            Pseudo Genes (internal stop)      :: 35 of 145
            Pseudo Genes (multiple problems)  :: 20 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP094235.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NY13320"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pNY13320-5"
     gene            333..554
                     /locus_tag="MPN36_RS15250"
                     /old_locus_tag="MPN36_15250"
     CDS             333..554
                     /locus_tag="MPN36_RS15250"
                     /old_locus_tag="MPN36_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299940.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKEY
                     RSAGRRNYLFSPEALKKLEKVITTKEGE"
                     /name="hypothetical protein"
     gene            555..812
                     /locus_tag="MPN36_RS15255"
                     /old_locus_tag="MPN36_15255"
     CDS             555..812
                     /locus_tag="MPN36_RS15255"
                     /old_locus_tag="MPN36_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002377879.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTNI
                     LELNSEEQKEHKGKLIAPSYRKAAKSAVPTGFLTKQPYIF"
                     /name="hypothetical protein"
     gene            complement(1182..1865)
                     /locus_tag="MPN36_RS15260"
                     /old_locus_tag="MPN36_15260"
     CDS             complement(1182..1865)
                     /locus_tag="MPN36_RS15260"
                     /old_locus_tag="MPN36_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_220577559.1"
                     /translation="MNMKMTKARHFGFLLYPDSIPENWREQLESLGVSMAVSPLHDKDK
                     NSDGTLKKPHYHVIYIARNPVTIESVRKKIKRKLGNSSVAHIEIIDHIKGSYGYLTHES
                     KDAKAKNKYVYDKKDILHINDFDIDRYVTLDESQKKELRNQLFDLINHCGIVNMKELAA
                     FLDWRGQEYGIANKRDVIDVIASNSNLFRMLFDANYQNGFRPQYAKRIDAETGEMIEGA
                     GNHVE"
                     /name="replication protein"
     gene            complement(1945..2094)
                     /locus_tag="MPN36_RS15265"
                     /old_locus_tag="MPN36_15265"
     CDS             complement(1945..2094)
                     /locus_tag="MPN36_RS15265"
                     /old_locus_tag="MPN36_15265"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_220577560.1"
                     /translation="MADSERLVVILPSEIKRKLEEKAKDKNLSMSTYVKLLVTEDLKKL
                     EKGN"
                     /name="hypothetical protein"
     Typing          715..890
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
ORIGIN
        1 cctcctcctt ccgaaatata cggacgctac cgctatttat ttcgtttagt tatcggctac
       61 acaatctaac acgaccgttc cgctaccagt cgccttacag gctccatttt cgctcctctc
      121 acgtgttttg acgattgcgc cgataactaa acgaaataga cgctaaatcg cttaaaacgc
      181 aaaatagacc ctttttagag cattgatttt ttctactagt catttcgata atataaaatt
      241 gaccacaaca cagaaaactt atattataag cttatgtcgt aagtccgacg tactcacgta
      301 atatagacgg acgttaaatg gaaggagcat aaatgagagt gataaaatca gaaaaattca
      361 ctttggccga aattatgacg accagcgaac ttgcacaaga acttgattac aaccaagctt
      421 acgttttgcg cttagcgaaa gaacatctga ctgaaggaaa agaatatcgc tctgccggta
      481 gaagaaacta tcttttcagc ccagaagccc ttaaaaaact agaaaaagtc ataaccacca
      541 aagaaggtga gtaaatgcct aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc
      601 tagaatcata ttccaatgat tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg
      661 acctctttga cctacttctc acaaatattc tggaattaaa ctctgaagaa caaaaagagc
      721 ataagggaaa actgatagca ccttcctaca ggaaggcggc taagtcggct gtgccaactg
      781 gttttcttac aaaacaacct tatatttttt gatatcacaa aaaaatccat tttggaccta
      841 ttttcggtca taatatagta cctacttttg gtcatagttt cgtccgtagt tttggtcata
      901 gtgactaaga ctttcacgca caaaaaaaga gctgaaatta acagctctta tcctttattt
      961 atttttgaat ttacacttcc accatgacgt ttcaatttca aaacttccgc tgttaccgca
     1021 gcatgataca ttcttttaaa tcttcctgag cttgccataa atatgacctc caaaattttt
     1081 ttattttaga agccccatac aatatgctag aatatactag caatctccca tgattgtttg
     1141 gggcttactc tctctttagt cggacgggtg taagcccctt tttattcgac gtggttaccc
     1201 gcgccttcaa tcatttctcc agtttcagca tcgattcttt tcgcgtactg tggacgaaat
     1261 ccattctgat aattagcatc aaacaacata cggaaaagat tgctattcga agctatcaca
     1321 tcaataacat ctcgtttatt agcgattcca tactcttgac ctctccagtc aagaaaagca
     1381 gccaactctt tcatattgac tattccgcaa tggttaatca aatcaaacaa ttgatttctc
     1441 aattctttct tttgactctc atctaaagtt acataacgat caatatcaaa atcattaata
     1501 tgcaaaatat cttttttatc atacacatac ttattttttg cttttgcatc ttttgattca
     1561 tgggttaaat atccatatga tcccttaatg tgatcaatta tttctatatg agcaactgaa
     1621 ctatttccta gttttctctt aattttcttt cttacactct cgattgttac agggtttcta
     1681 gcaatataaa tcacatgata atgtggtttt tttagcgttc catccgaatt tttatcttta
     1741 tcatgcaacg gactaaccgc catcgaaact cctaaactct caagttgttc tctccaattt
     1801 tccggaatag aatcgggata tagtaaaaaa ccgaaatgtc tagctttcgt cattttcata
     1861 ttcatgatat acttacctca attaaacact aacagtcaag cagaaacagt gcgccaacac
     1921 ttaagtttct gctctttttt gcgctcaatt tcctttctct aatttcttca aatcttctgt
     1981 tactaataat ttaacatacg tagacatact caaattttta tcttttgctt tttcttcaag
     2041 cttacgtttg atttcactcg gtaaaataac taccaatctt tcagaatccg ccatttttcc
     2101 aaccccttaa ttagttgtta acatcatgat aaggttttga caatagtttt gtcaatattt
     2161 ttttcatatt ttcaaaacgg cggtcaaaac ggcggtcaaa acggcggtcg tttttaaatg
     2221 gctcaatgcc ttgatagagt aagggttaag tcgttttttt aggaaatttg gcactcggca
     2281 ctttatgggg gggtcgtagt acggaagcaa aattcgcttc ctcgcccccc agttgtccca
     2341 aattccaaat ttttttcaaa ccagtagata acaaaaaaat caaaaacaca agacctcagg
     2401 gaatttcatt ccccgaaccc ctcgag
//
