LOCUS       NZ_CP093924             8335 bp    DNA     circular CON 27-MAY-2024
DEFINITION  Escherichia coli strain ST167 plasmid pGA_Eco4, complete sequence.
ACCESSION   NZ_CP093924
VERSION     NZ_CP093924.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26656496
            Assembly: GCF_022699465.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Batisti Biffignandi,G., Piazza,A., Marchesini,F., Prati,P.,
            Mercato,A., Abualsha'ar,A., Andreoli,G., Sassera,D. and
            Migliavacca,R.
  TITLE     Genomic characterization of a O101:H9-ST167 NDM-5-producing
            Escherichia coli strain from a kitten in Italy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8335)
  AUTHORS   Batisti Biffignandi,G., Piazza,A., Marchesini,F., Prati,P.,
            Mercato,A., Abualsha'ar,A., Andreoli,G., Sassera,D. and
            Migliavacca,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2022) Clinical-Surgical, Diagnostic and Pediatric
            Sciences, University of Pavia, Viale Brambilla, 74, Pavia 27100,
            Italy
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unycicler v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022699465.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 04:43:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,051
            CDSs (total)                      :: 4,927
            Genes (coding)                    :: 4,655
            CDSs (with protein)               :: 4,655
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 272
            CDSs (without protein)            :: 272
            Pseudo Genes (ambiguous residues) :: 0 of 272
            Pseudo Genes (frameshifted)       :: 116 of 272
            Pseudo Genes (incomplete)         :: 170 of 272
            Pseudo Genes (internal stop)      :: 53 of 272
            Pseudo Genes (multiple problems)  :: 58 of 272
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093924.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ST167"
                     /isolation_source="liver"
                     /host="Felis catus"
                     /db_xref="taxon:562"
                     /plasmid="pGA_Eco4"
                     /geo_loc_name="Italy"
                     /lat_lon="45.30989 N 9.50085 E"
                     /collection_date="2020-06-19"
                     /collected_by="Istituto Zooprofilattico Sperimentale della
                     Lombardia e dell'Emilia Romagna 'B. Ubertini', Pavia,
                     Italy"
     gene            join(8300..8335,1..288)
                     /locus_tag="MOO49_RS25025"
     CDS             join(8300..8335,1..288)
                     /locus_tag="MOO49_RS25025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            497..>928
                     /locus_tag="MOO49_RS25030"
                     /pseudo=""
     CDS             497..>928
                     /locus_tag="MOO49_RS25030"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            966..1484
                     /locus_tag="MOO49_RS25035"
     CDS             966..1484
                     /locus_tag="MOO49_RS25035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            1491..1724
                     /locus_tag="MOO49_RS25040"
     CDS             1491..1724
                     /locus_tag="MOO49_RS25040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2042..2461)
                     /locus_tag="MOO49_RS25045"
     CDS             complement(2042..2461)
                     /locus_tag="MOO49_RS25045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2542..2979)
                     /locus_tag="MOO49_RS25050"
     CDS             complement(2542..2979)
                     /locus_tag="MOO49_RS25050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            3239..4807
                     /gene="cea"
                     /locus_tag="MOO49_RS25055"
     CDS             3239..4807
                     /gene="cea"
                     /locus_tag="MOO49_RS25055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(4804..5145)
                     /locus_tag="MOO49_RS25060"
     CDS             complement(4804..5145)
                     /locus_tag="MOO49_RS25060"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_000058759.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            5193..5279
                     /locus_tag="MOO49_RS25065"
     CDS             5193..5279
                     /locus_tag="MOO49_RS25065"
                     /inference="COORDINATES: protein motif:HMM:NF014457.4"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_112036014.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVHV"
                     /name="colicin release lysis protein"
     gene            complement(5443..6642)
                     /locus_tag="MOO49_RS25070"
     CDS             complement(5443..6642)
                     /locus_tag="MOO49_RS25070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="WP_000948429.1"
                     /translation="MLSGFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACGT
                     RILGVKEFGCDNPDCQHVKYLTNSCGSRACPSCGKKATDLWTATQLNRLPDCDWVHLVF
                     TLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLNWHPHVH
                     VSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGMAMPESLSHITTESQW
                     RSLVLKSGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLD
                     HKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKP
                     EPVAKVCYAQMVKQFLSRDPFECVLCGCRMVYRRAIAGLNVSGLKKNARDISLLRYMPA
                     "
                     /name="IS91 family transposase"
     gene            complement(6652..6840)
                     /locus_tag="MOO49_RS25075"
     CDS             complement(6652..6840)
                     /locus_tag="MOO49_RS25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000957857.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVTTSIL
                     KRRGDTAALTLLQDTGV"
                     /name="hypothetical protein"
     gene            6953..7018
                     /locus_tag="MOO49_RS25080"
     CDS             6953..7018
                     /locus_tag="MOO49_RS25080"
                     /inference="COORDINATES: protein motif:HMM:NF014457.4"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_242806088.1"
                     /translation="MVQGGTIAPSSSSKLTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(7217..7375)
                     /locus_tag="MOO49_RS25350"
     CDS             complement(7217..7375)
                     /locus_tag="MOO49_RS25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(8045..8236)
                     /locus_tag="MOO49_RS25085"
     CDS             complement(8045..8236)
                     /locus_tag="MOO49_RS25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          7873..7960
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(7304..7859)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.878"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          279..1828
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cggttacttg aacgctgtga gggtaaacaa ctggcggtat ggatgcggcg ggtctgcctg
       61 ggggagccgg ttgcccgttc cggaaaactg ccgacactgg caccgccgtt actgcgtcag
      121 ctggccgcca tcggaaataa cctgaatcag acagcccgta aggtgaacag cgggcagtgg
      181 tcttccggtg accgggttca ggtggtggcc gcactgatgg ccatcgggga tgagctgcgc
      241 cggctgcgtc tggccgtcag ggaacagggg gcgcgggatg atagttaaat ttcatgccag
      301 gggaaaaggt ggtggcagtg gtccggttga ttacctgctg gggagggagc gtaaccgcga
      361 aggcgcaacg gtgcttcagg gtaatccgga agaagtccgg gaactcatcg atgccacgcc
      421 atttgcgaag aaatacacgt ccggtgttct gtcgttcgcg gagaaggagc tgccgccggg
      481 aggacgtgaa aaagtgatgg cgagctttga gcgtgtactg atgcccggtc tcgaaaaaaa
      541 tcagtacagc atcctgtggg tggagcacca ggacaaggga cggcttgagc tgaattttgt
      601 cattccgaac atggagctac agaccggaaa acgcctccag ccgtactacg accgcgcaga
      661 caggcctaga attgatgcct ggcagacgct ggtgaatcac cattacgggc tgcatgaccc
      721 gaacgccccg gagaaccgca ggacgctgac actccctgat aacctgcctg aaacgaaaca
      781 ggcgcttgct gagggcgtca cgcgaggtat agatgcactt taccatgccg gagagataaa
      841 aggccgtcag gatgtgattc aggcgctcac tgaggcgggg ctggaagtgg tcagggtgac
      901 gcgaagcagt atcagcattg cagatccgaa cggcgggaag aatatcaggc tgaaaggagc
      961 attttatgag caatcttttg cagacgggcg cggagttcga gaaaaagctg aaagagagag
     1021 ccgaatctac agagaaaatg ctgaacaacg agttcaggag gctcggcgaa tctgtaagcg
     1081 aggctgtgac atcaaacgag acgaaaatca gagacgctat agccctgttc acagcctcga
     1141 cagaggaatc gctggaaaaa caccgggaag gggtgaaaga ggcgatgatg cagcacagga
     1201 gggacgtgtt aaagctggca gggaatacgg gcatgatgtt actggggata gtctttctcc
     1261 tgtttaccgc gagtggcggg acgctctggt atcttggagg gaggatacag gcgaacctgg
     1321 aagaaatcag gaagcaggaa gagacattgc agaaactgaa cgcgaagaca tggggcgtgg
     1381 agtttgtgca ggacgggaac aggaaattcc ttgtccttcc gtacgggaaa tcagcggagg
     1441 tgattccctt tcaggggaaa gagtgggtac atctgaagga gtgacacaga gtgacagagc
     1501 tggaaacaca tttgctgaac gccttagagc agctgcaaca ggactatatg cagcggctga
     1561 gcgaatggga gagcgccttc gtggaattgc agaagatgtt ttcgcttacg caacgggaca
     1621 acgcgatgct gaacgagcgg gtcatgcagt tgagtcagca ggtgcagcac ttgagcgagc
     1681 agacagaacg cttgagccag ttatacagcg agaactggag ataagagagg aacggctgat
     1741 acaggagcgc gaacatgtgt tatccctgga acgggagcgt cagccggaaa tacaggaacg
     1801 cacgctggat ggcccttcgc tgggatggtg aaaccatgaa aaatggcagc ttcagtggat
     1861 taagtggggg taatgtggcc tgtaccctct ggttgcatag gtattcatac ggttaaaatt
     1921 tatcaggcgc gatcgcggca gtttttcggg tggtttgttg ccatttttac ctgtctgctg
     1981 ccgtgatcgc gctgaacgcg ttttagcggt gcgtacaatt aagggattat ggtaaatcca
     2041 cttactgtct gccctcgtag ccatcgagat aaaccgcaga aatcgtgtca gccagcagcc
     2101 tggatttttt cggggtaagg tctttgctgc ggtaatcagt acccgcaaat tgagttgtgc
     2161 cccggagtga gtttaaaaat tcctggctgt gaaactgctg aatgcgttgt tgcacatcac
     2221 tccgtgattt tcctgtcgct ttatctttag ctcccatatc ccagaactgc cgaaaaaaag
     2281 gcaccatcat tcgtgttgtg tcggcttttt gggtggaaaa gttgggatca aaaccgtcat
     2341 cagaagcata tacgtaatgc cgggtatgtt gttccacgct gccctttggg gtacaggctg
     2401 tgagagctac tgttgctgtt atcatggcga taagacaaag tttgtttccg gtacgtctca
     2461 tgagtgtctc cctaccttaa agtatttatt ctcggtgagt tattatccga agactatttt
     2521 atcatcagtc tctgacagcg ttcagcgacc gaacaacctg gaccagaatc cccgtttctt
     2581 ttccagttcc agtgcctgcc tgagctgggc tatctcattt tgtagctgtt cccgttctgc
     2641 ttcctggcgt ctgcgatcca tatcctgtgc ctgtttatcc tcaagcatca gcgtcaggca
     2701 ttgtttcagc tcattcagct cccggagaat gcgttctgtc tgctgatcat gtggattttc
     2761 tgcatgtccc tcactgtgcc tttctggtgt ctcattctgc tttaattcgc cgtatgcccg
     2821 gatcagttca ctggtttcaa actcccgtcg tccgtccctg ccggtacggt aactgacaag
     2881 gcctgcgcgc atatcacggt aaaactgtga acgcgatctg cctgtcattt ttagtgcgtc
     2941 ccggagtgtg tgccatgcca taaagtgaca gtgtcccata gatgtctcat ctcatagttt
     3001 cagtaaaaca taatgaggtc tgagaacggt aatgtttgtg ctggtttttg tggcatcggg
     3061 cgagaatagc gcgtggtgtg aaagactgtt tttttgatcg ttttcacaaa aatggaagtc
     3121 cacagtcttg acagggaaaa tgcagcggcg tagcttttat gctgtatata aaaccagtgg
     3181 ttatatgtac agtatttatt tttaacttat tgttttaaaa gtcaaagagg attttataat
     3241 ggaaaccgcg gtagcgtact ataaagatgg tgttccttat gatgataagg gacaggtaat
     3301 tattactctt ttgaatggta ctcctgacgg gagtggctct ggcggcggag gtggaaaagg
     3361 aggcagtaaa agtgaaagtt ctgcagctat tcatgcaact gctaaatggt ctactgctca
     3421 attaaagaaa acacaggcag agcaggctgc ccgggcaaaa gctgcagcgg aagcacaggc
     3481 gaaagcaaag gcaaacaggg atgcgctgac tcagcgcctg aaggatatcg tgaatgaggc
     3541 tcttcgtcac aatgcctcac gtacgccttc agcaacagag cttgctcatg ctaataatgc
     3601 agctatgcag gcggaagcag agcgtttgcg ccttgcgaaa gcagaagaaa aagcccgtaa
     3661 agaagcggaa gcagcagaaa aggcttttca ggaagcagaa caacgacgta aagagattga
     3721 acgggagaag gctgaaacag aacgccagtt gaaactggct gaagctgaag agaaacgact
     3781 ggctgcattg agtgaagaag ctaaagctgt tgagatcgcc caaaaaaaac tttctgctgc
     3841 acaatctgaa gtggtgaaaa tggatggaga gattaagact ctcaattctc gtttaagctc
     3901 cagtatccat gcccgtgatg cagaaatgaa aacgctcgct ggaaaacgaa atgaactggc
     3961 tcaggcatcc gctaaatata aagaactgga tgagctggtc aaaaaactat caccaagagc
     4021 caatgatccg cttcagaacc gtcctttttt tgaagcaacc agacgacggg ttggggccgg
     4081 taagattaga gaagaaaaac aaaaacaggt aacagcatca gaaacacgta ttaaccggat
     4141 aaatgctgat ataactcaga tccagaaggc tatttctcag gtcagtaata atcgtaatgc
     4201 cggtatcgct cgtgttcatg aagctgaaga aaatttgaaa aaagcacaga ataatctcct
     4261 taattcacag attaaggatg ctgttgatgc aacagttagc ttttatcaaa cgctgactga
     4321 aaaatatggt gaaaaatatt cgaaaatggc acaggaactt gctgataagt ctaaaggtaa
     4381 gaaaatcggc aatgtgaatg aagctctcgc tgcttttgaa aaatacaagg atgttttaaa
     4441 taagaaattc agcaaagccg atcgtgatgc tatttttaat gcgttggcat cggtgaagta
     4501 tgatgactgg gctaaacatt tagatcagtt tgccaagtac ttgaagatta cggggcatgt
     4561 ttcttttgga tatgatgtgg tatctgatat cctaaaaatt aaggatacag gtgactggaa
     4621 gccactattt cttacattag agaagaaagc tgcagatgca ggggtgagtt atgttgttgc
     4681 tttacttttt agcttgcttg ctggaactac attaggtatt tggggtattg ctattgttac
     4741 aggaattcta tgctcctata ttgataagaa taaacttaat actataaatg aggtgttagg
     4801 gatttaatta ttctttattg atataaaaag tcctagcaat ccaaatggga ttgctaggac
     4861 caaacaaagt agattatata gcataaatag gtttaatttt gctacggggg cgttatttag
     4921 gttttttctt ctttcgaaaa aatctttctt tatgaagtta aaagctatgt gttcaatagc
     4981 atattttgaa tatggacata gaatagtgct tatcactatt gcatatagca tcttatctga
     5041 cacaaggaaa taataccctt cgctgttttt tgttataagg tatatatata taagtgtgca
     5101 gtacaggcca aataaaatat tttttatgta gtatcttaag ctcataaatt aaacctcgcc
     5161 atatattctt ttcattttat aaggatcgag ttatgaggaa aagatttttt gtgggaatat
     5221 tcgcgataaa cctccttgtt ggatgtcagg ctaactatat acgtgatgtt cacgtatagg
     5281 aaattgaaaa accagtcgat accattgtcc acgccttatg ccacgtgagc ctttctgctt
     5341 ttcccaccac tccggtggtt tccctctctg tatggtcgat ttttataccg taacaggctg
     5401 ttttttatac tttcaccgat tcccgggcac acttagcctg tgtcaggccg gcatatacct
     5461 cagtagactg atatcccgcg cgtttttctt cagccctgat acattcagcc ccgcaatggc
     5521 ccggcggtat accatccggc agccacacag cacgcattcg aacgggtcac gactcaggaa
     5581 ctgtttcacc atttgtgcat agcacacttt cgccactggt tccggtttat ccatccccag
     5641 tgcacggtac acctgcggca gcttctctcc acacacacgg ttggcaagga acccgaagta
     5701 cctcaccatc ttaaaaaact tctccgggat gtgctgtttc agcctcgcga ccagctcacg
     5761 ctgtgtcagc gtttccgtcg ccgtttctcc cgttttgtgg tccaggtaac ggaagctcag
     5821 gcttgcccct ccgttgtaat gagccagtcg ggaagccgct attggtggct tcttcagata
     5881 acgacccagg tagcgtgccg tattccgccc tccggctgtc ttcttcgaca tgtacacatg
     5941 ccagtatttt ccgccggatt tcagcaccag gcttctccac tgtgattccg tcgtgatatg
     6001 tgacaacgac tccggcattg ccatcccctc tgaccacgct ttcagaagca gctgccgcat
     6061 attccacatc caccgtgaac gcatcgcgtc tttcaggaag ctcagctttt tccactgacc
     6121 atgcttattc agacctccac aggttacaga cacatgtaca tgcggatgcc agttgagacg
     6181 acggccatac gtgtggatgg cgcagaagat accgggttcc agcccccgtt ttcgggcggc
     6241 atacagcaga ttctccaccg ccagacggca cacgtcattc agcagccagc ggttgctttc
     6301 gaacaccggc cacagcgtgt ccggcagggt gaagaccaga tgtacccagt cgcagtcagg
     6361 aagacgattc agctgtgttg ctgtccacag gtctgtggcc ttctttccgc aggacgggca
     6421 ggcacggctg ccgcatgagt tggtcaggta ctttacgtgc tgacagtccg ggttatcaca
     6481 cccgaactct tttacaccca gtatccgtgt gccgcaggcc agcattttgg tgacggcttc
     6541 aacctcgata tcgcgcagac cgcccgcatc caggaaggac gtccagcact ggttggctgt
     6601 gaacagacgt ttcagagggc ggggagtaaa accggacaac atgaggatgg attaaacccc
     6661 ggtatcctgt agcagggtaa gggctgctgt atcgccacgg cgcttcagga tggaggtcgt
     6721 caccatcgca ggtggtgggt ccatgggttt gagcattttc atccagtccc attctcgttg
     6781 tgagagcttc tcagcaccaa cagagagcag aactgtatcg atacttttgc gggtcaacat
     6841 aaggggatga gtccttaata aaacaggtcc tcagagcata catattctgc ggggcgtgcg
     6901 gcaacaacag gacgaaccgg ggcggttttc aatttgcagc cgccaggctg ccgtggttca
     6961 gggagggacc atcgcaccat cctcctcttc taaactgacg gggatcgcgg ttcagtagaa
     7021 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     7081 aaaaaaccac cgctaccaac ggtggtttgt ttgccggatc aagagctacc aactcttttt
     7141 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     7201 tagtcgggcc actacttcaa gaactctgta gcaccgtttg tgccatcatc gctctgctaa
     7261 tccggttacc agtggctgct gccagtggcg ttaaggcgtg ccttaccggg ttggactcaa
     7321 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7381 ccagcttgga gcgaacgacc tacaccgaac tgagatacca acagcgtgag ctatgagaaa
     7441 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7501 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7561 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7621 tatggaaaaa cgcctgctac gtggccttct tcctgttcct ggtcttttgc tcacatgttc
     7681 tttccggcct tatcccctga ttctgtggat aactgtgtta ccgtttttgt gtgagtcagt
     7741 accgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga agcggaaaag
     7801 cgcctggacg tgcattttct ccttacgcat ctgtgcggca tttcacaccc ggcatggcgt
     7861 acttttcata caatccgcac tgatgccgca tggttaagcc agtatacact ccgctatcgc
     7921 tacgtgactg ggtcagggct gcgccccgac acccgctaaa acctgctgac gcgccctgac
     7981 gggcttgtca gctcccggca tccgctcaca gacaagctgt gaccgtctcc gggagctgca
     8041 tgtatcagag gttttcaccg tcatccccga aacgtgcgag gcagctgcgg taaagctcat
     8101 cggcgtggtc gtgaagcgat tcacaaatat cggcctgttc atctgcgtcc agttcgttga
     8161 gcttctccag cagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt
     8221 tttcctgttt agtcactgat gcctccgtgt aagggggatt tctgttcatg gggtaatgat
     8281 accgatgaaa cgcgagagga tgctcacaat acgggttact gatgatgaac atgcc
//
