LOCUS       NZ_CP093923            11208 bp    DNA     linear   CON 27-MAY-2024
DEFINITION  Escherichia coli strain ST167 plasmid pGA_Eco3.
ACCESSION   NZ_CP093923
VERSION     NZ_CP093923.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26656496
            Assembly: GCF_022699465.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11208)
  AUTHORS   Batisti Biffignandi,G., Piazza,A., Marchesini,F., Prati,P.,
            Mercato,A., Abualsha'ar,A., Andreoli,G., Sassera,D. and
            Migliavacca,R.
  TITLE     Genomic characterization of a O101:H9-ST167 NDM-5-producing
            Escherichia coli strain from a kitten in Italy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11208)
  AUTHORS   Batisti Biffignandi,G., Piazza,A., Marchesini,F., Prati,P.,
            Mercato,A., Abualsha'ar,A., Andreoli,G., Sassera,D. and
            Migliavacca,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2022) Clinical-Surgical, Diagnostic and Pediatric
            Sciences, University of Pavia, Viale Brambilla, 74, Pavia 27100,
            Italy
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unycicler v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022699465.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 04:43:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,051
            CDSs (total)                      :: 4,927
            Genes (coding)                    :: 4,655
            CDSs (with protein)               :: 4,655
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 272
            CDSs (without protein)            :: 272
            Pseudo Genes (ambiguous residues) :: 0 of 272
            Pseudo Genes (frameshifted)       :: 116 of 272
            Pseudo Genes (incomplete)         :: 170 of 272
            Pseudo Genes (internal stop)      :: 53 of 272
            Pseudo Genes (multiple problems)  :: 58 of 272
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093923.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ST167"
                     /isolation_source="liver"
                     /host="Felis catus"
                     /db_xref="taxon:562"
                     /plasmid="pGA_Eco3"
                     /geo_loc_name="Italy"
                     /lat_lon="45.30989 N 9.50085 E"
                     /collection_date="2020-06-19"
                     /collected_by="Istituto Zooprofilattico Sperimentale della
                     Lombardia e dell'Emilia Romagna 'B. Ubertini', Pavia,
                     Italy"
     gene            709..1059
                     /locus_tag="MOO49_RS24965"
     CDS             709..1059
                     /locus_tag="MOO49_RS24965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000493398.1"
                     /translation="MFTALNDKNTFGYPFEKIRNAIAVPSEKNVDAATSSGLEVLSRRY
                     DAFRQELDAAGELGNWEYDLDTYNHCIAVLQRYFTGNPSGLTERDARIYSHYLQTEHKG
                     FVKLAEELAADR"
                     /name="hypothetical protein"
     gene            1103..1258
                     /locus_tag="MOO49_RS24970"
     CDS             1103..1258
                     /locus_tag="MOO49_RS24970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_242806085.1"
                     /translation="MADWFIATEGVKVVKDSASVNATMFDRYGNADDFLDCFIIPARIV
                     GGTFWD"
                     /name="hypothetical protein"
     gene            complement(1218..2750)
                     /locus_tag="MOO49_RS24975"
     CDS             complement(1218..2750)
                     /locus_tag="MOO49_RS24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_097741024.1"
                     /translation="MFTEEEKIRAIELYFKYGKKLAPVVRELGYPSKRNLRRWIRSWEA
                     GGGTKESIRHKHRYSDEQKQVAVEHYLNHGCCLAFTSRALGYPCTDVLARWVNELYPDR
                     RRIFTSKANPVVPFESEVKRQAVMALCTRQVSASEIARRIGVSRAVLYKWKDEIIGNSA
                     YQTMRKHNEPSLEAERDALREEVARLNQEIRRRQMELDILKKAEEIIKKDPGISISHLN
                     NREKTKIADALRQTYPLTELLHVLGLARSSYFYHRAALKAGDKYATIRTMLTDIFNSNY
                     QCYGYRRLHAMLRHEGVRLSEKVVRRLMVEEQLVVSRNRRRRYSSYCGEIGPAPDNLIA
                     RDFKAEQPNQKWLTDITEFQLPAGKVWLSPVVDCFDGKVVSWSLSTRPDAELVNTMLDS
                     AVETLNAGERPVIHSDRGGHYRWPGWLERVNAAGLIRSMSRKGCSPDNAACEGFFGRLK
                     TEMYYGRKWSGITPEKFMQQVDAYIRWYNERRIKLSLGAVSPKMYRQQCGLE"
                     /name="IS3 family transposase"
     gene            complement(2826..3623)
                     /gene="istB"
                     /locus_tag="MOO49_RS24980"
     CDS             complement(2826..3623)
                     /gene="istB"
                     /locus_tag="MOO49_RS24980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase IstB"
                     /protein_id="WP_000544830.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQE
                     WNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVILLG
                     PPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYARVLI
                     LDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAILDRLL
                     HHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVKSGQHQ"
                     /name="istB"
     gene            complement(3623..4795)
                     /gene="istA"
                     /locus_tag="MOO49_RS24985"
     CDS             complement(3623..4795)
                     /gene="istA"
                     /locus_tag="MOO49_RS24985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="WP_242806086.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIAAQIGCSERTVRRYLKYPEPPA
                     RKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKSMGYTGGRSMLRYYIQPKRKMRP
                     SKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRRFHVFAAPKQDAEHT
                     YESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRACRPRR
                     ARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKETPEQRF
                     ALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRISLDDEL
                     RIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
                     /name="istA"
     gene            5541..6518
                     /locus_tag="MOO49_RS24990"
     CDS             5541..6518
                     /locus_tag="MOO49_RS24990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_000361611.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNTL
                     SRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVELP
                     FVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSAYYD
                     TERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPAPISL
                     ARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPRLKAPN
                     DESKENPLQPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
                     /name="RepB family plasmid replication initiator protein"
     gene            complement(6803..7543)
                     /locus_tag="MOO49_RS24995"
     CDS             complement(6803..7543)
                     /locus_tag="MOO49_RS24995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_001066953.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAYL
                     LAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLNGVRPFVRILSEKVR
                     ARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAVRRA
                     EADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFALDMAA
                     TLAVPFTGDGRDAAEILRTLPPLR"
                     /name="tyrosine-type recombinase/integrase"
     gene            complement(7664..7852)
                     /locus_tag="MOO49_RS25000"
     CDS             complement(7664..7852)
                     /locus_tag="MOO49_RS25000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001309252.1"
                     /translation="MASDTFSCRRRLSAGFFSVSACSWHYPNSPKNGKPALSPRHDWLN
                     TGSLLQNIPLRIWFYPQ"
                     /name="hypothetical protein"
     gene            8219..9388
                     /locus_tag="MOO49_RS25005"
     CDS             8219..9388
                     /locus_tag="MOO49_RS25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001072358.1"
                     /translation="MNQKWKTLIVSVLTPSSIISGIALIVLWGYFSRLDRLDIFFDVMN
                     IKSILVLVCCTTILSLIMIIFIFFITSFFIPIVIPYDIKNLPAYNKIQGNFLSVMMLSG
                     LFPMAFIYVLYCAFDFDQSVKDNSGWLSILSIGVLIVVISAIINTRYLEYDLSFKNNRM
                     KLLRRVQIYLIIPLSIALLVHLQVIPLEMVFRNIETSDKSINFKVIAELAFMSYFIFIL
                     TILPGLIYLKMNPQHKLSKRISSAFVASLMILLVISTQITVLPVIFTHSVIKLSGISDF
                     KIHSYIIKTSEYPEEFFSNAVWGKKNIKPGEYYSVQAVSMFTTNQFILLCPKDIIRFYR
                     ESWKFDLLNVDFDTNTRKKLQEEAAYCVPISAISVKRWDMPLQGSKPSN"
                     /name="hypothetical protein"
     gene            complement(<9614..>9789)
                     /locus_tag="MOO49_RS25010"
                     /pseudo=""
     CDS             complement(<9614..>9789)
                     /locus_tag="MOO49_RS25010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(10235..10507)
                     /locus_tag="MOO49_RS25015"
     CDS             complement(10235..10507)
                     /locus_tag="MOO49_RS25015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
                     /protein_id="WP_001322642.1"
                     /translation="MTPSPYPRNNFNTELIQSCMNGEHFSLLIEISPIRSKKNIMALKE
                     YLVDGYSKQESCERNNVSISYFCLCLKKLNYTHSIVSALKYFYRE"
                     /name="adhesin biosynthesis transcription regulatory family
                     protein"
     gene            complement(<11044..>11208)
                     /locus_tag="MOO49_RS25020"
                     /pseudo=""
     CDS             complement(<11044..>11208)
                     /locus_tag="MOO49_RS25020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     Typing          5542..6518
                     /name="IncFIB"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.569"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tattttgata actatatatt tttgattccg aaaacagata tttctttcag tgagtcaggg
       61 cttacactta aaatattaat attgtttgtg tgaagacata ttattcaata acgatggtga
      121 gaaatatctg taactttttc gcataatcta accatttact tgattgttca ttctgtgaac
      181 ataatctgtt tattatgtat caggttgatg atgtgattaa gcagtctgaa accagaggaa
      241 tatggagtca gaaattttca gttgtctttt aaaggcgtct gcaggatttt ttctccgttt
      301 ctgcctgtcc tggcattacc ccaattctcc caattctccc aattctccca gttacggatc
      361 ttcctctccc ccccacacaa cgccacttcc tgtaagcaca acatgtggtg ccggattcag
      421 ctgctgatga cactatatgt tgtgttattt ccctgacctg tgatgcgccg tgcaggggcg
      481 gaaaacagcg atatgattat ttcctcagcg tggtacactt ccggaagttg tgatattcaa
      541 gaaagtcgga tctgacggaa acggctctcc ggtaatttaa cggcgtggtt atatggatgc
      601 ttattatttc agtgtgtgaa agtcactttc taaacagagt ggcactcatc tgacgatgat
      661 tgaggtgtgg taaaattgca cggctaacta ttcaactgag ggaaggacat gtttactgcc
      721 ctgaacgata aaaacacttt cggttacccg tttgagaaga tccgtaatgc tatcgctgta
      781 cctagtgaaa aaaacgttga tgctgccacc agttccggtc ttgaagtcct cagccggagg
      841 tatgatgctt ttcgccagga gctggatgcg gccggtgagc tgggaaactg ggaatacgat
      901 ctcgatacct acaatcactg tattgcggtg ctgcaacgtt acttcaccgg caatccatcc
      961 gggctcacag aacgtgatgc ccggatttac agccattacc tgcagactga gcacaaaggc
     1021 tttgtgaagc tggctgaaga actggctgcc gaccgctgaa tacatacaga aggcgggatt
     1081 tctcaacaat aaaggaataa acatggctga ctggtttatt gcgaccgaag gcgtgaaggt
     1141 ggtgaaggac agcgccagtg tcaacgccac gatgtttgac cgttatggga atgcggacga
     1201 tttcctggac tgctttatca ttccagcccg cattgttggc ggtacatttt gggactgacg
     1261 gcacccagcg ataattttat acgccgctcg ttataccatc tgatgtaagc atccacttgc
     1321 tgcatgaact tttctggcgt gatgcccgac catttacgcc cataatacat ttccgttttc
     1381 agtctgccga aaaagccttc gcatgcggca ttatcaggtg aacatccttt acgggacatt
     1441 gagcgaataa gacctgccgc attcactctt tccagccagc ctggccagcg ataatgccca
     1501 cctctgtcac tgtgtatcac cggtcgttcg ccagcattta acgtttcgac agcgctatcc
     1561 agcatagtgt tgaccagttc agcatcaggg cgtgtactga gagaccagct cacaactttt
     1621 ccatcgaagc agtccaccac tggtgacagc cagactttac ctgcagggag ctggaactcc
     1681 gtgatatctg tcagccattt ctgattaggt tgctccgcct taaaatctct ggcgataagg
     1741 ttatccggag ccggtccgat ttctccgcaa tatgagctgt agcgacgacg acggttacga
     1801 ctgacgacaa gctgttcctc caccataagt ctgcgtacaa ccttttctga tagccgaacc
     1861 ccctcatgcc tgagcatcgc atgcaggcga cgatagccat aacactgata attactgtta
     1921 aagatatctg tcagcatcgt acgtatcgtg gcgtatttat caccggcttt cagtgcagcc
     1981 cggtgataaa aataactgct acgggcaagg cccagaacat gcagtaattc tgtcagggga
     2041 tatgtttgtc tcagggcatc agcgatcttt gttttttctc tgttgttcag gtgactgata
     2101 ctgatgcccg gatctttttt tatgatttcc tccgcctttt tcagaatatc cagctccatc
     2161 tgccggcggc gtatttcctg attcagtcgg gcgacttcct cccgcaacgc atcgcgttct
     2221 gcctccaggg aaggttcgtt atgtttacgc atagtctggt aagcactgtt gccgataatt
     2281 tcatctttcc atttatacaa taccgcacga ctgacaccaa tacgcctggc gatttcactg
     2341 gctgatactt gtcgtgtaca gagtgccatg acagcctgac gtttgacttc tgattcaaag
     2401 ggcacaaccg ggttggcctt actggtaaat atacgtcgtc tgtcgggata aagctcatta
     2461 acccagcgag cgagcacatc agtgcaggga taacccaggg ctctgctggt aaacgccagg
     2521 cagcagccgt ggttaagata atgttcaacg gcaacctgtt tttgctcatc ggagtaacgg
     2581 tgcttatgac gaatggattc ttttgtgcca ccacctgctt cccatgagcg gatccaacgc
     2641 cgcagatttc ttttcgaagg gtagcccagt tcgcgtacga cgggagccag cttcttacca
     2701 tatttgaaat aaagctcaat ggcgcggatt ttttcttctt cagtaaacat gttttatctc
     2761 ctgagagtcc aggaaatcgt ccgcagcccc tatttgccat tttcatgccc gctaagatac
     2821 tttcgttact gatgctgtcc gcttttcacc atttcatcat cactgattgg cgttgtgttt
     2881 ttggtcagca ctccagcttt acgtttctct tttaaccggt aactctctcc tttgatattc
     2941 agcgtggttg agtgatgtag caaccgatcc agtatcgctg ttgccagcac gtgatctccg
     3001 aacatttctc cccagtctgc gaaccctttg tttgacgtca gtatgatgct cgctttttca
     3061 tatcgacggt tcagtagccg gaagaacagg ctggcttcct ctctgttcat cggcagatag
     3121 cctatttcat ccaggatcaa cacccgggca taactcagtt gctgcagctg acgctccagc
     3181 cggttttcct gtttcgcttt catcagtgtc gcgatcagtc tgtccagtgg cataaacagt
     3241 acccgatgtc ccgcatccac tgctttcacg ccaagagcta tggccagatg agtttttccg
     3301 acaccaggag gtcccagcag gatcacgttt tcgctgcgct ccacgaacgc cagaccagcc
     3361 agttcccgga caaccttacg gtcgatgccc ggctggaagg taaagtcgaa ctgctccagc
     3421 gttttgaccc acggcagacg agcctgcttc agcctggact ccataccgcg ctgatgcctg
     3481 ccgttccatt cctgctgtag cgccatgcac aggaactccc ggtagttcag ctctttttta
     3541 gctgcctgtt ccagcaggct ttcaacgtga taactcagat gctccatttt caggcgactc
     3601 agcaggactt ccagctcatg catcacaaca gctcctcata ggcactcagt ggtcgatgtt
     3661 ccacctgact gacctgctgc cagagcgggg cgtgatgctc cggcactgtc tgccagccag
     3721 acgatgccga acagaggcga tgtgaggcca ccagtttctc attactgtag atccgcaact
     3781 cgtcatccag cgatattcgt atcgataccg gctgaccaca cagcgcttcg ggaacgctgt
     3841 aacgattacc accaacctcg atatagctgt cccaggacac atggcggata tcgaagtaac
     3901 tggtatcgaa gtccgtatcc ggtaacggct gcagatgttc ctgctccagc gcgaagcgct
     3961 gttccggcgt ttctttgaac tggcgaagtt cccgtttgtc agccacatcg gctatccatt
     4021 gctccagttg ttgattaaca tgagtgaagc tgtcgaacct gcggtaccgg acgaagaagt
     4081 tctccttgag gtatttcacc atccgctcaa ccttaccttt tgttctggct ctgcgtggac
     4141 ggcatgcccg tggcaggaag ttatagtggt cggccagcaa caggaatccg gagttgaaca
     4201 cgactttccc gttgttattt ttcagcaccg cagccttctg gttatcgacc agcaccgttt
     4261 tcacacaacc accgaagtag cggaaggcgc gaaccagtga ttcgtaggta tgctcagcat
     4321 cctgttttgg tgcggcgaag acatggaagc ggcgggagaa ccccagcgta ttaaccgcaa
     4381 agttaacttt gcaccgttgc ccggcaacct ccacctcaac ttcgccccag tcatgctgga
     4441 gctggtatcc aggctgagtt tcgaagcgaa ctgttctttt tgacggacgc attttacgtt
     4501 tgggctggat gtagtaacgc agcatggaac gtccgcccgt atagcccatt gacttaatct
     4561 ccgcgaagat aacctcgcta ttccagacat tctctgccag gcgcatgtcg atgtaatcca
     4621 taaacggttt cagcttaacc attttgtggc gggtctttct ggctggcggt tcagggtatt
     4681 tgaggtagcg tctgaccgtc cgttcagagc aaccaatctg agccgcaata tcgacaatgt
     4741 acgcgccctg ctggcgcatt tgctttatca tgtaaaagtc ctctctgctc agcatgttga
     4801 tgtcctttct ggtgtgagaa ccttaaggaa acaacatgtt gggtggagcg gacaatccaa
     4861 atggtgaatt accgtcttat atcactggcg ctgacacctg tagtttgtat acacaatccc
     4921 tggcattgaa acacacatat gaagtgtact tcggatatga ccgtacgctc aggaagtacg
     4981 gcagattccg tcaaaaatta cctgtaaaga cagacatcag aaaaaacagt gctcgccgca
     5041 gaccggtgac cgggcgaagc ccgcgaaccg gcgaggaagc gcaatgtatc tgtagttaca
     5101 gggagcaggt acgccctgaa gccctttcat aaaacggttg tgtgcgctgt ccttctggaa
     5161 tgatttatcc acatatccgg aggcccgatc cagtaaacag atccatgaat gatcaacaaa
     5221 ggatccatta aagatcccca tatcgctgca aaccttgtca ctcatgggcc gggaccacga
     5281 tcacataagc tgtggcatgt tactgataaa ctgtaacatg ctaatgataa gctgtattca
     5341 gtaatccata tactgaagta agttaatgac ataaactatg gtcagtatgc cagactcagt
     5401 tgttaaatac aggctgcagg tttttcttca gtcagttagc ggagttctga cacacgattt
     5461 tctgtttatt cttttactgt ccacaggctg gaggctttct ggaaaacgaa aattcagaca
     5521 tcaaaaaact gttcggcgag gtggataagt cctccggtga gctggtgaca ctgacaccaa
     5581 acaataacaa caccgtacaa cctgtggcgc tgatgcgtct gggcgttttt gtaccgaccc
     5641 ttaaatcact gaagaacagt aaaaaaaata cactgtcacg tactgatgcc acggaagagc
     5701 tgacacgtct ttccctggcc cgtgctgagg gattcgataa ggttgagatc accggccccc
     5761 gcctggatat ggataatgat ttcaagacct gggtggggat cattcattcc tttgcccgcc
     5821 ataacgtgat tggtgacaaa gttgaactgc cttttgttga gtttgcaaaa ctgtgtggta
     5881 taccttcaag ccagtcatcc cgcaggctgc gtgagcgcat cagcccttcc ctgaagcgca
     5941 ttgccggtac cgtgatttcg ttttcccgta ccgatgagaa gcacacccgg gaatatatca
     6001 cccatctggt acagtcagct tactacgata ctgagcggga tattgtgcag ttacaggccg
     6061 atccccgcct gtttgaactg taccagtttg acagaaaagt ccttctccag cttaaggcga
     6121 ttaatgccct gaagcgacgg gagtccgccc aggcactcta cacctttata gagagcctgc
     6181 cccgggatcc ggcaccgata tcgctggcgc ggctgcgtgc acgcctcaat ctgaagtctc
     6241 ctgtattttc ccagaaccag acggtcagac gggcaatgga gcagttgcgc gagattggat
     6301 atcttgatta cacggagatc cagcgggggc ggacaaaatt cttctgtatt cactaccggc
     6361 gtccccggtt aaaagcgccg aatgacgaga gtaaggaaaa tccgttgcaa ccttcacctg
     6421 cggaaaaagt cagtccggag atggcggaga agcttgccct gcttgagaaa ctgggcatca
     6481 cgctggatga cctggaaaaa ctcttcaaat cccgctgaac ataaactgta gtcagtgaag
     6541 agcgctcctt tactgactac agcttatatt aaccggtgca gtgagtggtc tgctcactgc
     6601 agtttatatt ctgtttcctg cagtgctgtc tgtagctgag ctgccatctg cctgtccctt
     6661 acgtaagtga tcccgtaacc tgatgctgag gcattgctcc cttcataaaa catgacttac
     6721 tcactacagc ttatatacat gctccagctt atgttatgtc tgttctgctg accacagctt
     6781 atataaggaa gcgcgtatgc cgtcacctca ggggaggcag tgtacgcagg atctccgcag
     6841 catcccgccc gtcacctgtg aaaggcactg ccagcgtggc agccatatcc agtgcaaaca
     6901 ctctggtata aacctccatc gaacgtggat ccctgtgacc agccagtgcc tggatgactt
     6961 tccggggctg gcggtgatag agcatgtgca tgatatagct gtgccggaag gtgtgtggtg
     7021 tgaccggaat cgaaaagtgt accccgtcag cttcggcccg tctgacagcc tgcttcagcc
     7081 agttgcgcat ggtttcgtcg gtcacggccc ataatggttc acgacgacgg ggccgggtgg
     7141 tgatcatcca gctttccatc tgcctgacat agcttatatc tgtcagcggc accagtcgca
     7201 cttcatcttt tggcgggcgt ccgcgtcgcg cacgcacttt ttcggacagg atccgcacaa
     7261 acggtcttac tccattcagg tcaaatgatt ccggcgtcag catccgggct tcgccaatac
     7321 gcattccggt attccagagg gtggcgaaca gcatatggtg acgctgatcc ggcatataga
     7381 agagaagggc actcacttcc ggggccagca ggtaggccgg tgtggccccc gtggaagtgg
     7441 ccattcttct cagggagagc gctgttgcaa aatccacccc cggcgcaatg ggtaacaggg
     7501 agacgcgttc tggtgaattc tgcaggggaa tgacattgtt catgagttat ttttcacgta
     7561 gcacacgcac tgtttatgga tacaggtatt ctgacctgaa cgcccggatc cttccacgac
     7621 aataaatcgc agcgcgcgga tccttttgtt tgcttatcac gcattattgc gggtaaaacc
     7681 aaatacgtaa aggtatgttt tgcagcaggg atcccgtatt aagccagtcg tggcgcgggc
     7741 tgagggctgg tttgccgttt ttgggagaat tggggtaatg ccaggaacag gcagatacgg
     7801 agaaaaatcc tgcagataac cttctacgac aactgaaggt gtctgacgcc atattcttag
     7861 ctacttctca gttttaaggc ggcttagtca caacataagg ttatgcataa taagcatatt
     7921 ttattatgtt atgttatggt cttgttcatg catgaactgt tatctattca gtcactttta
     7981 acgttttata tcatactgca tatgacacaa tcatcttcta tattcagatt aaaaatatta
     8041 tgacgacgat caaataaatt tagtgaaatc attacttaca ataatatgtt tatgacataa
     8101 tttatcaaaa atatccgaca accttcctga aaggttactg cgaggagtca aacaatgaag
     8161 tgtaataact tattcactct tttttatgct ctctttatag caaaaccagg aagttaaaat
     8221 gaatcagaag tggaagacgc taatagtttc agtattaaca ccatcaagta ttatatcagg
     8281 gattgcgttg atagttctct ggggatattt cagtcgtctg gatcgacttg atattttctt
     8341 tgatgtgatg aatattaaaa gcatcttggt attggtttgt tgtacaacta ttctatcatt
     8401 gattatgatc atttttattt tttttataac atcttttttt ataccaatag ttattcctta
     8461 tgatataaaa aatcttcctg cttacaataa gattcagggt aattttttat ctgtaatgat
     8521 gttatctggc ctcttcccca tggcctttat atacgtgtta tactgtgcat ttgattttga
     8581 ccagagtgtt aaagataact ctggctggtt atctatttta agtataggag tactaatagt
     8641 tgttatttca gccataataa acacgaggta tcttgaatat gacttatcgt ttaaaaataa
     8701 tagaatgaaa ctgttgagac gtgttcagat ctatcttatc attccgttgt ctattgcttt
     8761 actggtgcat cttcaggtaa ttccactgga gatggttttt aggaatattg aaacttcaga
     8821 taagagtatt aatttcaaag tgatagctga actggctttc atgtcatatt ttatttttat
     8881 tctgacaata cttcccggcc tcatttatct aaaaatgaat ccacagcata aactttctaa
     8941 aagaattagt tctgcatttg tcgcatcctt gatgatatta ttagttatat cgactcaaat
     9001 aaccgtatta ccagtaattt ttacccattc agttattaag ctctcaggta taagtgactt
     9061 caaaatacat agctacatta ttaaaacaag tgaatatccg gaggagtttt tttccaatgc
     9121 agtatggggt aaaaaaaata taaaaccggg ggaatattac tctgtgcagg ctgtttctat
     9181 gtttacgaca aaccagttta ttctcctttg tcccaaggat attataaggt tttatcggga
     9241 aagctggaag ttcgacctgc ttaatgtaga cttcgataca aataccagga agaaactcca
     9301 ggaagaagca gcatattgcg taccaatttc tgcaatatct gtaaaacgat gggatatgcc
     9361 tcttcagggc tcgaaaccct caaattagaa ggccgctcgt gcgactgtat tagatagtaa
     9421 tgcggtatgg aaaaatagtg atggcaagat gaacttgact tggctaatca aataccggcg
     9481 tagtgattag gaaacagggc tcggataaca ttattgcgcg gttcattgaa cctactgatg
     9541 acgccggata tatagtcgta aaccgtttcg aaccagtact tatatcacca ggagagtcca
     9601 tatatgacca ccgtttacag taaaaacggt atctgttttc tgatccctcc agcgtcaaac
     9661 taccgttgta atggtgaggt ctggtgctat tgtaataatt cagaatataa tctctgattt
     9721 atttccgaac ctcgcccttg cctgcgtaac catccgtcgc ccccatcctg ttttcagact
     9781 gcggaaaaag gcaagttttt atccccccac agagattcaa tcatatatcg cgtgttttgg
     9841 aagtgacagt tcttattcct gagaatactg catgtgcaac acactcggca tccatcgata
     9901 ccacttttat ctccaatagg caggataaaa aacaagactg tgaattaaag taatcggcaa
     9961 atgaatgagt tagaagatac tgaaaatgaa tcaaggacta accccgtagg gaatgaaaat
    10021 aataaaagaa catcttgttt ttattttaat gaatgagcat gttcttttat attaataaca
    10081 aaacctatca ttaaacaaaa tgatgaatga cagagttttc tttaaaatag cttatattat
    10141 cttacagtag ctgacatagt gcaatataaa ttatcagcta tgccataacg gcttagcagt
    10201 gtctaagcgt aagaatcaag acttcagtta cagtttactc acggtagaaa tatttaagag
    10261 cactaacaat actgtgtgta tagtttagtt tttttaggca taggcaaaaa taactaatgg
    10321 aaacattgtt cctttcacaa ctttcttgct ttgaataacc atcaaccaaa tactccttaa
    10381 gagccatgat gtttttttta cttctgattg gtgatatttc aattaaaagt gaaaagtgtt
    10441 ctccattcat acatgattga attagctctg tattaaagtt atttcttgga tagggggagg
    10501 gagtcatata ttatctaacc ttatttttct attaaagaat tgaaatagaa tagacagaaa
    10561 tgctattgga gtaagtattt gatagtaggt aatttacgct cctatataga ctattgttac
    10621 tcatataata ttttggggtg gcttatgttg tgacagcgcc agttgttaaa aacgttgtac
    10681 cgtcatacgg gataatatag aacaaccaat aacgattact ggtctatgac caccgccaga
    10741 gatacagaaa agctttatct gtaatgagct caactatata tcccgtcaac tgctggttca
    10801 gagcgttgtc gtaaaaatcc tgtgcaaata aattagtcat atatcaagcc atttacgcgg
    10861 taactggcag gtctgaaatt taacctcctg acgatgcagg cttgcataca tccatagttt
    10921 tactttggta tgtgactcta agtatgtaga tcatcagtcc ggcatggaac gctgttttga
    10981 cccgctaaaa tccttacgga aaagtaagga tgtggatttg cccctatatt tccagtcatc
    11041 ggttcgtctg agaatgacgt gcaactgcgc acgcgacacc cggagacaac ggctgactaa
    11101 gcttactccc catccccggg caataagggc gcgtgcgcta tccacttttt tgcacgccca
    11161 tattcaacgg cttctttaag gagttcattt tccatcgttt ttttgccg
//
