LOCUS       NZ_CP093918             4991 bp    DNA     circular CON 18-MAY-2024
DEFINITION  Enterobacter cloacae strain SD21 plasmid pSD21_4kb, complete
            sequence.
ACCESSION   NZ_CP093918
VERSION     NZ_CP093918.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26686306
            Assembly: GCF_023238665.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Wang,S., Ju,X., Dong,N., Li,R., Zhang,R., Huang,Y. and Zhou,H.
  TITLE     Emergence of mcr-8.2 in an Enterobacter cloacae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Wang,S., Ju,X., Dong,N., Li,R., Zhang,R., Huang,Y. and Zhou,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAR-2022) Department of Clinical Laboratory, The
            Second Affiliated Hospital Zhejiang University School of Medicine,
            88 Jiefang Rd., Hangzhou 310009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. v3.15.1.; Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023238665.1-RS_2024_05_18
            Annotation Date                   :: 05/18/2024 04:44:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,963
            CDSs (total)                      :: 4,848
            Genes (coding)                    :: 4,775
            CDSs (with protein)               :: 4,775
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 51 of 73
            Pseudo Genes (internal stop)      :: 10 of 73
            Pseudo Genes (multiple problems)  :: 16 of 73
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093918.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="SD21"
                     /host="Homo sapiens"
                     /db_xref="taxon:550"
                     /plasmid="pSD21_4kb"
                     /geo_loc_name="China: Shandong"
                     /collection_date="2021-10"
     gene            complement(join(4637..4991,1..47))
                     /locus_tag="MQH03_RS24770"
                     /old_locus_tag="MQH03_24770"
     CDS             complement(join(4637..4991,1..47))
                     /locus_tag="MQH03_RS24770"
                     /old_locus_tag="MQH03_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032489494.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASVDQCEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(47..238)
                     /locus_tag="MQH03_RS24775"
                     /old_locus_tag="MQH03_24775"
     CDS             complement(47..238)
                     /locus_tag="MQH03_RS24775"
                     /old_locus_tag="MQH03_24775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032489493.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            654..820
                     /locus_tag="MQH03_RS24845"
                     /pseudo=""
     CDS             654..820
                     /locus_tag="MQH03_RS24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            1150..2208
                     /locus_tag="MQH03_RS24780"
                     /old_locus_tag="MQH03_24780"
     CDS             1150..2208
                     /locus_tag="MQH03_RS24780"
                     /old_locus_tag="MQH03_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508929.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_042109829.1"
                     /translation="MAIYAVWNNKGGVGKSYLTFQLASEYARQNPTKKVLVVDLCPQAN
                     SSSMLLGGMINGENTLNQIHSAIQRKTISGYIEDRIRSPYIPTNTGSSYVINVSTHNSK
                     VPNNLYLVTGDEQLELQASRVVGASFPGPHDAWRTVHLWINDLISDICNSWNNEDSCVF
                     IDCNPSFSIYTELALTAAERLLIPFSADGSSKRAVKAVLSLVYGERRHQNEPQSDFYLN
                     SNRYSMSLPQIYMYVGNRLTQMNNSSASAFKTVVTEIGDEIWKVWQTKPSLFCIHPNGS
                     STPNNKRSFRNMFQYEVNDANTASVVSGALGIPIATLTAGNKTVSGKIIKVNQTQLDKQ
                     VPNIVALAGMIE"
                     /name="ParA family protein"
     gene            complement(2238..2495)
                     /locus_tag="MQH03_RS24785"
                     /old_locus_tag="MQH03_24785"
     CDS             complement(2238..2495)
                     /locus_tag="MQH03_RS24785"
                     /old_locus_tag="MQH03_24785"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042109830.1"
                     /translation="MGSNNNQNKDLQDKQADLLDQTYTLGWMDGYAEGYDDAIEDTQNA
                     LNKLENENELLTEVVESKDNVIKELASSAGRALQAVKKNK"
                     /name="hypothetical protein"
     gene            2913..3344
                     /locus_tag="MQH03_RS24790"
                     /old_locus_tag="MQH03_24790"
     CDS             2913..3344
                     /locus_tag="MQH03_RS24790"
                     /old_locus_tag="MQH03_24790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_042109831.1"
                     /translation="MKIPAHLKHKPIIQVENYDRIDGPHADDTDAMGLSVGIAQWNTPG
                     WTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICITMDYSKNGRLSSSDDFPVTRST
                     DNPELAHHTEFMKKELKQNKSNLDVSLKRLSTELKRLGY"
                     /name="DUF6530 family protein"
     gene            complement(3438..3842)
                     /locus_tag="MQH03_RS24795"
                     /old_locus_tag="MQH03_24795"
     CDS             complement(3438..3842)
                     /locus_tag="MQH03_RS24795"
                     /old_locus_tag="MQH03_24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="WP_008321396.1"
                     /translation="MTILVTIQAQLVVGEEQVIKSLAPEGMLAAVFEDDGRTGYFYALD
                     ESVEGNPIMDAVHIYNAEDVSDGHIPSDVKIGWSEDSQKCVLLINGYPHGAFDFVGKNG
                     YCRSGYPPPINKVWSLSGHEWSDSVDDFFR"
                     /name="DUF2251 domain-containing protein"
     Typing          360..762
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.857"
                     /coverage="99.0"
                     /evalue="7.72e-165"
ORIGIN
        1 ctcttacccg tcagtcgggc tgcctcacta atagacactc tggccatcag ttaccatcct
       61 gtaatgcatc cagtctcgag gacagtgtac ggaaaagatg ctccgcatca tcgtggagtt
      121 tctcacaaag gtcggcctcg acatcaagat caagctcgtt aagcttctcc agcaggagca
      181 gagattgatg ctggatgaat ttagccatgt tcagcgctgt tttctgttgt ttcgtcataa
      241 gaggcctcag agcgtcagtg ctcagtgtag ggatgttctc ttttccgctt cctcgctcac
      301 tcagtcgcta cgctcctgcc gttcggctgc ggcgagcggc aggagcatgc gtatgaaagc
      361 tcgagaaaac cgagcgatca cgtaatttag aaaagggaag gccgcacagc ggccgttttt
      421 ccataggctc cgcccccctg acaagcatca cagaaacgga cgctcaaatc agtggtggcg
      481 aaacccgaca ggactataaa gatatcaggc gtttccccct gatggctccc tcgtgcgctc
      541 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca
      601 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg
      661 aaccccccgt tcagtccgac tgctgcgcct gttccggtaa ctttcgactt gagtccaacc
      721 cggaaggcac gacaaaacgc cactggcagc agccactggt aaggggtgta caagaattac
      781 tggtgcagag ttcttgaagt tacagcctaa ctacagctaa actgaaagga cagatttggt
      841 ggcagcggtc ttgtacaccc tgttaccacg gttcagaagt tccccaactt actgatcctt
      901 cgaaaaacca cctccccagg tggttttttc gtttacagag caaaagatta catcccgcag
      961 gttaggatct caagaagatc cttttaatcg gaattcgaca actccagata agtcatggtg
     1021 agccagccaa ccccttcgac tgaatatcgt tccaaaaaaa ggcagtcagc tgaaagaaac
     1081 taacagtaga acaataatct ctagtaccct gaatctaagc taacaacaaa actagattag
     1141 gagttataga tggcgatcta tgccgtatgg aataacaagg gtggtgtagg gaaaagttac
     1201 cttacttttc agttagcctc tgaatatgcc agacagaacc ctactaaaaa ggtcctggtg
     1261 gttgacctgt gcccacaagc gaactcgtcc tctatgttac ttggtggaat gataaatggc
     1321 gaaaacacac taaaccagat tcattcagca atacagagaa agacaatatc tggatatatt
     1381 gaggatagga tcagaagtcc atatattcct accaacactg gttctagcta tgtaataaat
     1441 gtatctacac acaatagtaa agttccaaat aatctatatc ttgtcactgg cgatgaacaa
     1501 ttagaactcc aagcatcaag agttgttggg gcatcatttc caggtcccca tgatgcatgg
     1561 cgtactgttc acctctggat taatgattta atcagtgaca tttgcaactc atggaataat
     1621 gaagatagct gcgtattcat tgactgcaac cctagcttct ctatttatac agagttagct
     1681 ttaaccgcag cagagcgcct tttaattcct ttctctgccg atggttcatc aaagagagca
     1741 gttaaggctg tactttcttt agtttatggt gagcgtcgcc atcagaatga accacaatca
     1801 gatttctatc ttaatagcaa tcgttattct atgtcgctac cacaaatata tatgtatgtt
     1861 ggaaatagat taacgcaaat gaacaattca tctgccagtg catttaagac ggtagtaacc
     1921 gaaattggtg atgaaatttg gaaagtatgg caaacaaaac caagtctgtt ttgtattcac
     1981 ccaaatggga gctcaacgcc aaataataaa agatctttca ggaatatgtt tcagtatgaa
     2041 gtaaatgatg caaacacagc atctgtcgta tctggagcct taggtattcc aatcgcgaca
     2101 ttaactgcag gaaataaaac tgtttcagga aaaataataa aggttaatca gacgcagcta
     2161 gataaacaag tccccaacat tgtagctctc gctggtatga tcgaataaaa tagcaaggcc
     2221 cttaaaaggg ccttttttta tttgtttttc tttactgctt gcagcgccct gccagcacta
     2281 gaggccaact ctttgattac attatcttta ctttcaacaa cctcagtaag caactcgttt
     2341 tcgttttcca gtttattcaa agcgttctga gtatcttcaa tagcatcatc atacccttcg
     2401 gcatagccat ccatccaacc aagagtatac gtttggtcca aaagatcagc ttgcttatcc
     2461 tgcagatctt tattctgatt attgttagag cccatgtttt ttcctcattt gttggcgcat
     2521 ctgagttttc aaaccaggag gaattccatt tctaagtttc tcacttagtc tgactggtgg
     2581 tgtgtttcct gagactggat tacagtcagc atccaccccg acatctttct ttttatagtc
     2641 gccaatattc ctaacgttat ctgtagtcat agaaaaccac gcccattgct gtgaataacc
     2701 gaactataaa ccagaaaaaa aacgtacggc ttatcataac acttgacaca aaaaaacacc
     2761 gctactctcc aagcaaaaaa cataaaattg gaaaaacaaa atcacaagca catgataaca
     2821 atagatatta atatttactt acatttagtg agttttataa ttagaaaaac aatgtaattt
     2881 aataatcaca aacattacct aaggacgaac tgatgaaaat acccgcgcat ttgaagcata
     2941 aaccaattat tcaagtcgaa aattacgacc gcattgacgg cccacatgcc gatgacactg
     3001 atgctatggg gctatctgta ggtattgctc aatggaacac tcctggatgg actgagcttt
     3061 cggcaaaggt ttggcgaaac acaggagaaa aatggtcaag acaatcagaa gagctgcctc
     3121 ttcaccgggt aatcgacctt gcaactctga tctgtatcac catggactac agtaaaaatg
     3181 ggcgactttc atccagtgat gattttcccg tgactcgctc aactgataac cctgagctag
     3241 cacatcacac tgagtttatg aaaaaagagt taaaacaaaa taaaagcaat cttgatgtaa
     3301 gcctcaaacg cctctcgact gaattgaaga gacttggcta ttaaaactag ctttagaaat
     3361 gtctacctac ttcacagacg aatttaccat aacatccgtt tttcgaacca taatggcagg
     3421 ctatagcctg ccgtaattca ccggaaaaaa tcatccacag agtcgctcca ctcatgcccg
     3481 gaaagagacc acaccttgtt tataggggga ggataaccac tcctgcagta cccgtttttt
     3541 cccacgaaat cgaatgcccc gtgcgggtat ccattgatca ggagtacgca cttctgactg
     3601 tcttctgacc agccaatttt aacatcagaa ggaatgtgac cgtctgaaac gtcttcggca
     3661 ttgtagatgt gtactgcgtc cataatcggg tttccctcga cggactcatc gagcgcatag
     3721 aaatacccgg tccggccatc gtcttcgaac acggcggcca gcatgccctc aggagcgagg
     3781 ctctttataa cctgttcctc cccgacaacg agctgggcct gtatcgtcac taaaatggtc
     3841 atcaggtctg ttctcctttc cttggttgct tcggcagcca gctttcagac aagcggataa
     3901 tttaccataa catccgttat cagcatcgtc agaaacgcgc tgagggcgtt tctgacggga
     3961 aaccggtaaa acaggcaaca aaccacccga aaacccgcca gaaacgcgca ggctctgttt
     4021 ttatggcatg catgagtatg cacgcaagtg catagcgcga atattcacgc cattcagaca
     4081 gggtaagtcc tgtttccggc gttcttccca tccgaaaacg gtgaacgggg acgcaattta
     4141 ttgcgtggcc gtgaagcgtt gccggcgcag ccggaaaacc ggttttcagc acgttcagga
     4201 atcgggttcg tcgacggccg gcaatcacgt tatggcaagt cgccggacga ctcaccacac
     4261 gattcaccgg cgactctgca ctagagtcgt acctagagtc gccgcgacta aaaaacaacc
     4321 aaacggggag tcgggtctag agtcgggtcg ttattaactc attgttttta tagccctgag
     4381 tcgttaccga ctctatgacg actcgccggg gactctatga cgagtcgtca tagagtcgga
     4441 tctatataaa taaaatttaa aaaacgaaat ctcaggaaag taaactactg atattaaata
     4501 caaatctgca aaattttaat tttgcgtggg gtgtgggtat ttttagtggt gaggagcgtt
     4561 cagagatctg actatttttg ggaaggttca ggaactcccc gggatggccg gagaggaaaa
     4621 ctgaagcatg acagggtcag gattttttcc agaatttcca ccatggcgcc ggggattcag
     4681 ttaattttgc ctggtgattt tcaagaagga gcatggcctg tttcaggctg tcaatgtgcg
     4741 aatctttgga caaaagtaaa gtgttcaggt gttcaatctc ctgtttcaga tgacgaatca
     4801 cctgttcaca ctggtcaaca gaggctgtat cgcgctgttc agagacctgt ccagaatggt
     4861 gttcaccctt cagttgttca aagggaccaa agacacgtaa aagctctgaa gtatctacca
     4921 tttttgcatt gcgctcaccg ctacaggtgg acaggtcacc tgctttaatt aacctgtgca
     4981 gtgttgttct a
//
