LOCUS       NZ_CP093505             6265 bp    DNA     linear   CON 12-JUN-2024
DEFINITION  Klebsiella pneumoniae strain VA04 plasmid pVA04-6.
ACCESSION   NZ_CP093505
VERSION     NZ_CP093505.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26642714
            Assembly: GCF_030061865.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Marcoleta,A.E., Hormazabal,J.C. and Araya,P.
  TITLE     Complete genome sequencing and analysis of carbapenem-resistant
            Klebsiella pneumoniae isolated in Chile
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6265)
  AUTHORS   Marcoleta,A.E., Hormazabal,J.C. and Araya,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2022) Biology, Grupo de Microbiologia Integrativa,
            Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425 Nunoa,
            Santiago, RM 780000, Chile
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: unicycler v. 0.4.9b; Canu v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 176X
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030061865.1-RS_2024_06_12
            Annotation Date                   :: 06/12/2024 05:32:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,708
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 167
            CDSs (without protein)            :: 167
            Pseudo Genes (ambiguous residues) :: 0 of 167
            Pseudo Genes (frameshifted)       :: 64 of 167
            Pseudo Genes (incomplete)         :: 111 of 167
            Pseudo Genes (internal stop)      :: 15 of 167
            Pseudo Genes (multiple problems)  :: 21 of 167
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093505.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="VA04"
                     /isolation_source="Isolated from cerebrospinal fluid of a
                     36 years old male"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pVA04-6"
                     /geo_loc_name="Chile: Metropolitan Region"
                     /collection_date="2018-12-05"
                     /collected_by="Instituto de Salud Pblica de Chile"
     gene            complement(<1..777)
                     /locus_tag="MO409_RS28475"
                     /old_locus_tag="MO409_28450"
                     /pseudo=""
     CDS             complement(<1..777)
                     /locus_tag="MO409_RS28475"
                     /old_locus_tag="MO409_28450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(1171..1596)
                     /locus_tag="MO409_RS28480"
                     /old_locus_tag="MO409_28455"
     CDS             complement(1171..1596)
                     /locus_tag="MO409_RS28480"
                     /old_locus_tag="MO409_28455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_097470724.1"
                     /translation="MALVSVTEAARLTGKNRTTIHRYIKQGKLSQISDATNTKKVDTSE
                     LIRVFGSLTATHATLSQSDAMQHDATPKTDQSVALLQQKIALLEQLLENKNKELQRQDE
                     HIDSLKQAMLLIESKLPTTPEPVTPVPAKKSWQFWKK"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1604..2560)
                     /locus_tag="MO409_RS28485"
                     /old_locus_tag="MO409_28460"
     CDS             complement(1604..2560)
                     /locus_tag="MO409_RS28485"
                     /old_locus_tag="MO409_28460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_128502104.1"
                     /translation="MSELVVFKANELAVSRYDLTEHETKLILFCVAKLNPTIENPTRDE
                     LTVKFSCSEYARTMGLSYENAWGRLNSATSDLFKRSVELIYPTGAVSKRIFNWTEYAEF
                     NREEQTVTLVFSSYIQPLLFHLKKFIKYNLEHVKAFENKYSMRIYEWLLKELSQRKTHR
                     GNIEISIKEFKFMLMLEKNYPLYAELNRWILKPVTNDLNTYSNMKLTIEKRGRPADTLI
                     FQVELDKQIDLVTELEKDPASKKEDKTIRLTPENRLHEGLKTTLHDALTAKIQLTGFEA
                     KFLSDMQSKYDLNGSFSWLTQKQRATLEKILAKYGRI"
                     /name="replication initiation protein"
     gene            complement(3156..3611)
                     /locus_tag="MO409_RS28490"
                     /old_locus_tag="MO409_28465"
     CDS             complement(3156..3611)
                     /locus_tag="MO409_RS28490"
                     /old_locus_tag="MO409_28465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_097470722.1"
                     /translation="MKNEKQAKWLTDRVQYIRGLKAPNEQQRLLVILHDKTDKTAQEIK
                     TLSLLIQAERAAEKAQEARTKVMNLIHAEKRAEAKAARKARDHALYQSAGLLIMAGLVD
                     SQTGKPVDDPAKLLGALASLHELSRDNPRWEEWKLKGQVLLNQKKED"
                     /name="conjugal transfer protein TraD"
     gene            3777..4892
                     /gene="mobA"
                     /locus_tag="MO409_RS28495"
                     /old_locus_tag="MO409_28470"
     CDS             3777..4892
                     /gene="mobA"
                     /locus_tag="MO409_RS28495"
                     /old_locus_tag="MO409_28470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_097470721.1"
                     /translation="MASYHLSVKTGGKGKAASHADYIAREGKYAREKDSDLEHKESGNM
                     PAWAAHKPAEFWKAADTFERANGCTYREIEIALPRELKPEQRLELVRDFIRQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERMNDGIDRDPQQYFKRANSKEPERGGAKKVRFG
                     ETPTERREYLIAQRERWATLQNNHLERYQHPDRVDARSLKAQGIDREPERHLGAGQVQR
                     FDTDQLQAILERRDAERQVQQCCDERDSVIDVTTSLREALIERDTLTPKQAQQPEPEQD
                     AVSGRVFDFEKEPDKLNELVSDAMKDIQEEIDLQSLVNDAMAEFQGIHQEMVRQQERTA
                     LAEKQRQQEKERQRIAEQKQQKPDKGWSFSR"
                     /name="mobA"
     gene            4905..5489
                     /locus_tag="MO409_RS28500"
                     /old_locus_tag="MO409_28475"
     CDS             4905..5489
                     /locus_tag="MO409_RS28500"
                     /old_locus_tag="MO409_28475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA modification GTPase"
                     /protein_id="WP_163621336.1"
                     /translation="MSDEIKQIAALIGHQQAQEKRVAALIDEFQAESAKLSRQTAQLAQ
                     VIHELDSASGKMTDTIRQSVNAALTQVEKELKQTGLAQQKPAVNALEQVVTAANASVSA
                     MRREMSRYTWKSAIYIVLTMLAVMGSCLWGMVCFIDSGYDRIAKMERMEAVWQKKAPLA
                     DISTCDGKPCVKVDTSQSYGDKKDTYLIIKQ"
                     /name="tRNA modification GTPase"
     gene            <5579..5728
                     /locus_tag="MO409_RS28505"
                     /old_locus_tag="MO409_28480"
                     /pseudo=""
     CDS             <5579..5728
                     /locus_tag="MO409_RS28505"
                     /old_locus_tag="MO409_28480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            5822..>6265
                     /locus_tag="MO409_RS28510"
                     /old_locus_tag="MO409_28485"
     CDS             5822..>6265
                     /locus_tag="MO409_RS28510"
                     /old_locus_tag="MO409_28485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Qnr family pentapeptide repeat protein"
                     /protein_id="WP_164525329.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLG"
                     /name="Qnr family pentapeptide repeat protein"
     ARG             5787..6265
                     /name="QnrB10"
                     /gene="QnrB10"
                     /gene_name="quinolone resistance protein (qnr)"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic"
                     /identity="99.38"
                     /coverage="70.8"
     Typing          complement(1605..2560)
                     /name="rep_cluster_1155"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.97"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3778..4889
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.367"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taactcggca ctcagacccg tattatcgcg gttttcctga taacgggtga ataccccgat
       61 gcccggctga ttgctgccgc gcgtgtcgta gttatcaata agctgagtgc cgttggcgaa
      121 gttcatcgcc ccgcccaccg tccagttttc ggccggtaaa cggattgccc cgaacagacc
      181 ggttgccaga cggtgattac tgctgacggt atcatactgg ctgaaaaccc cgacgcaata
      241 atcactctgg cctaactgac aacggctctg acttaagctg tttaagctgc tctggtacag
      301 gtctaacact ttaaaaccgc gacgaccggt atccgccatc gctttggatg aattggttgc
      361 atcgactatc gttactaccg gcggctctac cggcggcacc gggtatttca ctttccacac
      421 ggtggcatgg gtatcacctt catcagtatc tgagtacccg actactacag tcccgtccgc
      481 cgagacccca ctggcagagg aatacccgct gttgtcggct ttaagggtgc cgagaccggt
      541 cattttggtg tctccctcac gccacacaaa ggcattatag tcgccgctgt cagtcctgga
      601 ctctcccact actactgtgc cgtccgccga gaccccactg gcataggaat accctccatt
      661 ccccgggcgt aaagttccca ggtcagtttt actgtccaca atgggggctt ctgccgcgga
      721 ggcataccca cttaatgcca ggcttaatgc cagggctaca ctgctttttt ttaacatatt
      781 gttatcgctc caaaaagaag tcacttacgt gacaaaataa aattttttat ttgtaccgct
      841 gcggtgtggg ggcagcgatg gtccgggtag aggtcatcct ggttggctat agtggcgggg
      901 ggtttaaaat aaaattttaa agtattttga tataaacaaa caaatattta tttttttttt
      961 tttgttataa attaaatgat taccgttctg ctattgctca gatttaacat aagggtgctt
     1021 aagcgcactt ccgtttaggc tttgctcctg aattttttct caaccgcgca aaatgccctg
     1081 ttttctccct gtaaaaagcc cgatttttat gttgccaatt tgttatcaaa atgacgattt
     1141 cggtttcagg gtgaaaatgc cgccaaccgg ctattttttc cagaattgcc acgatttttt
     1201 ggctggtact ggtgtaacgg gttcaggtgt tgtcggcagc ttggattcga ttaacagcat
     1261 tgcttgtttc aggctgtcta tatgctcgtc ctgacgttgt aattctttat ttttgttttc
     1321 aagcagttgc tctaaaagtg caattttttg ttgtaacagt gcaacggatt gatctgtttt
     1381 aggtgttgca tcgtgctgca ttgcatcgct ttgcgacagt gttgcatgtg tcgccgttag
     1441 tgagccgaat acacgaataa gctctgatgt atctactttt tttgtgtttg ttgcatcaga
     1501 aatttgcgac agttttcctt gcttaatata gcgatgtatt gttgttctgt tttttccagt
     1561 tagtctggct gcctctgtaa ctgataccag tgccatagat acctcagatc cgtccgtatt
     1621 ttgccaatat tttctctagc gtggctcgtt gtttttgagt cagccatgag aacgagccgt
     1681 ttagatcata tttgctctgc atgtcactca gaaatttagc ttcaaagccg gtaagctgaa
     1741 tttttgcagt taaagcatca tgcaatgttg tttttagccc ctcatgaaga cgattttcag
     1801 gtgttaaacg gattgtctta tcttcttttt ttgatgctgg atctttttct agttcagtca
     1861 caaggtcaat ttgcttatcc agttcaactt gaaagatcag tgtgtcagca ggacgaccgc
     1921 gtttttcaat agtcaacttc atattgctgt aagtgtttaa gtcatttgta actggtttta
     1981 ggatccagcg attcaattct gcatataaag ggtagttttt ttcaagcatg agcataaact
     2041 taaattcctt gatacttatc tcaatgtttc ccctatgcgt ttttctttgt gaaagctcct
     2101 ttagtaacca ttcatatatt cgcattgagt atttattttc aaaggcttta acatgctcaa
     2161 ggttgtattt aatgaatttt tttaaatgaa ataaaagtgg ttgaatatat gagctaaata
     2221 ctaatgttac agtttgttct tctctattga actcagcgta ttccgtccaa ttgaaaattc
     2281 gtttagaaac tgctcctgta ggataaatta attcaacaga gcgtttaaat aaatcactag
     2341 ttgcactgtt caatcttccc caagcatttt cataacttaa gcccatagtg cgcgcatatt
     2401 cagaacatga gaatttaact gttaattcat cccttgttgg gttttcaatt gtagggttca
     2461 actttgcaac gcaaaacaga attagcttgg tttcatgttc agttagatca taacggctta
     2521 ctgctaattc atttgcttta aacacaacta attcagacat gccaatcacc attatgtagt
     2581 aatttaaatc gccacataaa tcataataat gtagcaatta aaatcaacta cattttttat
     2641 gtttgattgt gaataaccta atttttccta catctttccc actgctagcc taatttttcc
     2701 tacatccagc ctaattttcc ctacatttaa tgattttttt tttattaaaa aacaatgatt
     2761 taaaatcact agaataaaga aagaataata aagataaaaa aagaatagat cccagccctg
     2821 tggataactc agaaaactgc cgtcgatttg ccgtagttaa aaggatgtcg caaacaaggt
     2881 tttctccttt tcaaaacaaa cctaaaacac ctcagtagct ccctcgcttt gcccgggcaa
     2941 ctcgctgacc ctttcttttg tctccgacca tccagccact gcgccgctgt tttttccgtg
     3001 tcattgagtt cgcttcgctc acggctcaat caggaaagtg gctgtatgcg cttctgtgcc
     3061 acgctttcag tgttaggcga tgaaatccat taccgagcct gtaaaacctc gccacggact
     3121 tcctgtggcg ttttagaggg tattaacacc ctgttctaat cttccttttt ctgattgagt
     3181 aatacctgcc ccttcaattt ccattcttcc cacctgggat tatcacggga taattcatgc
     3241 aagctggcta atgcgcccag tagtttggcg gggtcatcca ccggtttacc cgtctgactg
     3301 tccactaacc ctgccataat cagtaatccc gccgattgat aaagggcatg gtcacgcgct
     3361 ttacgggcgg ctttggcttc tgctcgtttc tcagcgtgta tcaggttcat cactttcgtg
     3421 cgggcttcct gtgctttttc tgctgcacgc tctgcctgaa ttaacaatga gagcgtcttt
     3481 atttcctgag cggttttatc cgttttatcg tgcaatatca ccaataaacg ttgttgttca
     3541 ttcggtgctt ttaaaccacg gatatactgc actctgtctg tgagccactt agcctgtttt
     3601 tcgtttttca tctttaccac tcatgctgtt tttcgaatgg aataattatg ctcattattg
     3661 gcaactcagg tcaaattcca ctacaatccc tgtcatcctt tttattgaca gtgcgcactt
     3721 agacgtttct tcactatgtt cagaaacggt gcgcgcctgc ggcgggcgaa aacactatgg
     3781 cctcttatca cctcagcgtc aaaaccggcg ggaaaggcaa agccgcttcc catgccgatt
     3841 atattgcgcg tgagggaaaa tatgcccgtg aaaaagactc cgaccttgag cataaggagt
     3901 ccggcaatat gcccgcatgg gcagcgcata aaccggctga gttctggaaa gccgctgaca
     3961 cgtttgaacg tgccaacggc tgcacctacc gcgagattga aatcgccctg ccacgagagc
     4021 tgaaacctga gcagcggctt gagctggttc gcgattttat ccggcaggaa attggcgacc
     4081 gtcatgcgta tcagtttgct atccataacc cgaaagcagc gatagccggt ggcgaacagc
     4141 cgcatgcgca tatcatgttc tctgaacgga tgaatgacgg cattgaccgt gatccgcagc
     4201 agtattttaa acgggcgaac agtaaagagc ctgagcgtgg cggcgcgaaa aaggtgcggt
     4261 tcggcgaaac gccgaccgaa cgcagggaat accttatcgc gcagcgggaa cgctgggcaa
     4321 cattacaaaa taaccatctt gagcgttatc agcatcctga ccgtgtggat gcgcgttccc
     4381 tgaaagcaca gggtatcgat cgtgagccgg agcggcatct gggcgcggga caggttcaac
     4441 gcttcgatac tgaccagtta caggccattc ttgaacgcag ggatgccgaa agacaggttc
     4501 agcagtgctg tgatgaacgt gacagcgtga ttgacgtgac cacttcgtta cgggaagccc
     4561 ttattgagcg ggacactctg acgccaaaac aggcgcagca gccggagcct gaacaggacg
     4621 ccgtttccgg cagggtgttc gattttgaaa aagagcctga caagctgaat gaactggtca
     4681 gcgatgccat gaaggacata caagaagaaa tcgaccttca gtcacttgtt aacgatgcga
     4741 tggcagagtt tcagggcata catcaggaga tggtcaggca gcaggaacgc accgcgcttg
     4801 ctgaaaagca aagacagcag gagaaagaac ggcagcgtat tgccgaacag aaacaacaaa
     4861 aaccagacaa aggctggtca ttttcacgat agacaggatt ttacatgagc gacgagatta
     4921 aacagattgc tgcccttatc gggcatcagc aggcacagga aaaacgcgta gccgcgctga
     4981 ttgatgaatt tcaggcggaa tcagccaaat taagccgcca gacagcacaa ttggcgcagg
     5041 taattcatga actggacagt gcctccggta agatgaccga cacgatcagg caatcggtga
     5101 atgccgcttt aacgcaggtt gaaaaagaac tcaaacaaac gggattggcg cagcagaaac
     5161 ccgccgtcaa tgcgctggaa caggtggtta cagcggctaa tgcgtctgtg agtgcaatgc
     5221 gacgtgagat gtcgcgctat acgtggaagt ctgcaattta tattgttctg acgatgcttg
     5281 cggtcatggg gagctgtctt tgggggatgg tctgttttat cgacagtggc tatgaccgaa
     5341 ttgcaaaaat ggagcgcatg gaagcggtct ggcagaaaaa agccccgctg gctgatatct
     5401 ccacgtgtga tggaaaacct tgcgtgaaag tggataccag tcagtcatat ggcgataaaa
     5461 aagacacgta tctgattatc aaacaataaa aaacggcgct gaatatgcct caaatgccac
     5521 cggcgatttc cagccgagtg ttgaatgtct tcggcgtgga ttataaaagc catttacgct
     5581 ggatctgcgt gaattccagc agcagcaaga gaaagacttt ctacaaacca gcttacagca
     5641 ggctaaattt aaccagaaaa aagccgctga attactgggt ctgacttatc atcagctacg
     5701 tgccttgctg aagaaacacc agatctaata cgcatttgca ggtgtcatac tggcgggttt
     5761 gacgcataac gtcataaggt ttaccatggc gtcattactg tataaaaaca caggcataga
     5821 tatgactctg gcattagttg gcgaaaaaat tgacagaaat cgcttcaccg gtgagaaagt
     5881 tgaaaatagt acatttttta actgcgattt ttcaggtgcc gacctgagcg gcactgaatt
     5941 tatcggctgc cagttctatg atcgcgaaag tcagaaaggg tgcaatttta gtcgcgcaat
     6001 gctgaaagat gccattttca aaagctgtga tttatcaatg gcagatttcc gcaacgtcag
     6061 tgccttgggc attgaaattc gccactgccg cgcacaaggc gcagatttcc gcggtgcaag
     6121 ctttatgaat atgatcacca cgcgcacctg gttttgcagc gcatatatca ctaatactaa
     6181 tctaagctac gccaattttt cgaaagtcgt gttggaaaag tgtgagctgt gggaaaaccg
     6241 ctggatgggg actcaggtac tgggt
//
