LOCUS       NZ_CP093500             6871 bp    DNA     circular CON 12-JUN-2024
DEFINITION  Klebsiella pneumoniae strain VA32 plasmid pVA32-7, complete
            sequence.
ACCESSION   NZ_CP093500
VERSION     NZ_CP093500.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26642715
            Assembly: GCF_030061815.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6871)
  AUTHORS   Marcoleta,A.E., Hormazabal,J.C. and Araya,P.
  TITLE     Complete genome sequencing and analysis of carbapenem-resistant
            Klebsiella pneumoniae isolated in Chile
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6871)
  AUTHORS   Marcoleta,A.E., Hormazabal,J.C. and Araya,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2022) Biology, Grupo de Microbiologia Integrativa,
            Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425 Nunoa,
            Santiago, RM 780000, Chile
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: unicycler v. 0.4.9b; Canu v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 202X
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030061815.1-RS_2024_06_12
            Annotation Date                   :: 06/12/2024 05:24:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,570
            Genes (coding)                    :: 5,428
            CDSs (with protein)               :: 5,428
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 51 of 142
            Pseudo Genes (incomplete)         :: 94 of 142
            Pseudo Genes (internal stop)      :: 16 of 142
            Pseudo Genes (multiple problems)  :: 19 of 142
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093500.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6871
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="VA32"
                     /isolation_source="Isolated from bronchoalveolar lavage of
                     an 81 years old female"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pVA32-7"
                     /geo_loc_name="Chile: Metropolitan Region"
                     /collection_date="2019-01-04"
                     /collected_by="Instituto de Salud Pblica de Chile"
     gene            complement(146..280)
                     /locus_tag="MO414_RS28340"
                     /old_locus_tag="MO414_28305"
     CDS             complement(146..280)
                     /locus_tag="MO414_RS28340"
                     /old_locus_tag="MO414_28305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_165451377.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPEKHGKTPLAAATGKQIEQEIC"
                     /name="replication initiation protein"
     gene            972..1292
                     /locus_tag="MO414_RS28345"
                     /old_locus_tag="MO414_28310"
     CDS             972..1292
                     /locus_tag="MO414_RS28345"
                     /old_locus_tag="MO414_28310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_045265547.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLETGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1501..>1932
                     /locus_tag="MO414_RS28350"
                     /old_locus_tag="MO414_28315"
                     /pseudo=""
     CDS             1501..>1932
                     /locus_tag="MO414_RS28350"
                     /old_locus_tag="MO414_28315"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1970..2455
                     /locus_tag="MO414_RS28355"
                     /old_locus_tag="MO414_28320"
     CDS             1970..2455
                     /locus_tag="MO414_RS28355"
                     /old_locus_tag="MO414_28320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_013631087.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            2459..2671
                     /locus_tag="MO414_RS28360"
                     /old_locus_tag="MO414_28325"
     CDS             2459..2671
                     /locus_tag="MO414_RS28360"
                     /old_locus_tag="MO414_28325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3519..3743
                     /locus_tag="MO414_RS28365"
                     /old_locus_tag="MO414_28330"
     CDS             3519..3743
                     /locus_tag="MO414_RS28365"
                     /old_locus_tag="MO414_28330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            3727..3993
                     /locus_tag="MO414_RS28370"
                     /old_locus_tag="MO414_28335"
     CDS             3727..3993
                     /locus_tag="MO414_RS28370"
                     /old_locus_tag="MO414_28335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000262326.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRILCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4050..4295)
                     /locus_tag="MO414_RS28375"
                     /old_locus_tag="MO414_28340"
     CDS             complement(4050..4295)
                     /locus_tag="MO414_RS28375"
                     /old_locus_tag="MO414_28340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080090623.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNRTLPKAVKQQVRERV
                     LRSIQDEREARQAQEIRREKYEAAETTFTWQPRRRR"
                     /name="hypothetical protein"
     gene            4622..6310
                     /locus_tag="MO414_RS28380"
                     /old_locus_tag="MO414_28345"
     CDS             4622..6310
                     /locus_tag="MO414_RS28380"
                     /old_locus_tag="MO414_28345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_080090624.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNTMNDALSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKTRKGAKDYGHDYHPAPKTEDIKGLGDLKKGTPKTPMQGGGGRRKRWIGDKGRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQIKGPDPKGRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            6320..6577
                     /locus_tag="MO414_RS28385"
                     /old_locus_tag="MO414_28350"
     CDS             6320..6577
                     /locus_tag="MO414_RS28385"
                     /old_locus_tag="MO414_28350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_022644819.1"
                     /translation="MGLKLDLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGCFDVKNEWVSLLQPYFKHKINLSDSSYFVSFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            6662..6811
                     /locus_tag="MO414_RS28390"
                     /old_locus_tag="MO414_28355"
     CDS             6662..6811
                     /locus_tag="MO414_RS28390"
                     /old_locus_tag="MO414_28355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_080090625.1"
                     /translation="MKKAKTIFLFILIASGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1..6871
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          778..865
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          complement(209..755)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.948"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1283..2787
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.618"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acgaaaaaac cgcctgggga ggcggtttga tcgaaggata agtcagttgg ggaactgctt
       61 aaccgggtaa cagtctgagc aagagacgtg tcaccaaaac tgtccttcca gtgtagccgc
      121 agttaggcca ccacttcaag aactctcaac atatctcttg ctcaatctgc ttaccagtgg
      181 ctgctgccag tggcgtttta ccgtgctttt ccgggttgga ctcgagacga tagttaccgg
      241 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      301 cgacctacac caagccgaga taccaacagc gtgagctatg agaaagcgcc acgcttcccg
      361 aagggagaaa ggcggacagg tatccggtaa gtggcagggt cggaacagga gagcgcacga
      421 gggagcttcc agggggaaac gcctggtatc tttaagtcct gtcgggtttc gccacctctg
      481 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaagcgcct
      541 ccggcgcgac ctttttcccg ggctttgtct tgttgcacac agctctttcc ggcgttatcc
      601 cctgattctg tggataaccg tatcgccgct tttgagtgag cagacaccgc tcgccgcagc
      661 cgaacgaccg agcgcagcga gccagtgagc gaggaagcgg aagagcaccg gtacctgcat
      721 gttctcctta cgcatctgtg cggtatttca caccggtcat ggtgcacttt tcatacaatc
      781 cgcactgatg ccgcataatt aagccagtat acactccgct agcgctacgt gactggttca
      841 gggctgcgcc ccgaaacccg ctaaaagcga ctgacgcgcc tgcggcttgt ccaacaccgt
      901 gccgcccggg aaaaatttcc cgcccgtccc ggtgttatca ggaggctact ctggcagata
      961 aacgcaacaa gatgctcacg atgtgggtga cggaagacga acaccggcgg ctgctcgagc
     1021 ggtgcgacgg caagcagctg gcggcgtgga tgcgtcagac ctgcctcgat gagaagcctg
     1081 cacgtgccgg cagactgccg tcgatatcgc cggcgctgct gcgccagctt gccggtatgg
     1141 gtaacaacct gaaccagatc gcccggaaag tgaatgccgg tggtgccggt catgatcggg
     1201 tgcagatcgt ggccgccctg atggcaatcg acgcgggact ggagcggctg cggcatgcgg
     1261 tgctggaaac agggggggat gatgatcgtt aaattccatc cccgggggcg tggtggtggt
     1321 gccggtccgg tggattacct gctgggaaaa gaccggcagc gcgagggggc gaccgtgctg
     1381 cagggaaagc cggaggaagt ccgggagctt atcgatgcct cgccgtatgc gaaaaagtac
     1441 acctccggtg tgctgtcgtt cgcggaggct gaactgccgc ccgggcagcg ggagcagatc
     1501 atggccagct ttgagcgggt tctgatgccc gggctcgata aagaccagta cagcattctg
     1561 tgggtggagc acaccgacaa ggggcggctg gagctgaatt tcctgatccc caacactgaa
     1621 ctgctgaccg ggaaacgcct gcagccgtac tacgatcgcg ccgaccggcc gcgcatcgat
     1681 gcctggcaga ccgtggtgaa cggcagactg gggctgcatg atccaaacgc cccggagaac
     1741 cggcggttac tggtcacgcc gtccgcactg ccggaaacga agctggaagc cgctcaggcg
     1801 attacacggg gtttactggc cttagcctca tccggggagc tcaaaacgcg cgaggacgtc
     1861 acaggggcgc tcacagcggc aggttttgag gtggtccgca ccacaaaaaa cagtatcagc
     1921 attgccgacc cggacggggg gcgaaacatc cgactgaagg gagccattta tgagcagtct
     1981 tttagcgctg gcgaaggact tggagcagca atcgaaagcg cagcagcaga gtaccggcga
     2041 gatgctgaaa gccgcattca gcgagcacga gcagtctgta aaagcggaac tgagcgcaag
     2101 cgcgaagaga atcagcgacg ccatcagcgc ccacgagcag ggtatgaccg cggcgatgca
     2161 gtcgaaccgg ctgagcgtgc tgcgtatggt tggccggacg tggctgacca tcaccctggt
     2221 ctcggtactg ctgatcgcca cgagcgggag cattctgtgg tggcaggggc agcagatcac
     2281 cgggaattac cagactatcc gggcgcagga gcggacgcag gccatgctgt cggagaagaa
     2341 ccacggcgtg cagctgtcgc tctgcgggga gaagaaactg tcctgcgtca aagtgaaccc
     2401 gaaagcggga gcttacgggg aggaagggaa ctggatggta ctggagagga aatagcccat
     2461 gacggaactg gaaaaacagt tgctgaccgc attagagcag ttacatcagg actactcgca
     2521 aaggctggac gagtgggaga gcgccttcgc ggaatggcag aagatgtctg gtcttatgca
     2581 acgggagaac gcggcgctga acgagcgcgt cacgcgcttg agtcagcagg tggcgaactt
     2641 gagcaggcag ttacagcgtt tgagccagta gtgcagcggc atgaaatggc ggtggccgcc
     2701 gaacgggcat acgagcagaa acagcatgaa aaagagctgg ctgaaacccg ttcccggcag
     2761 cggagctata acggtccgac gctgggctga ctgcgcgcat ccagtgacaa accccaagaa
     2821 gaagggctca taaatttccc gctaaatacg gtcgtgatgt ttccatcaca gcgtatacaa
     2881 cggagggatt atgagactgt ttaacgtgat tgagacggtg ctgaactgcc tgtacatggc
     2941 gctggagatc tgggactggc tggagaagca ttttcctgtt tttaaggcgt tcatccggat
     3001 tctggcctga aatcagaccg tatgcatctg gctgcatgac catgcagccg ggtaaaattt
     3061 atctgtcacg aacggggcgg tgtatcgggt ggtttgttgc cggttttacc tgactgccgc
     3121 cccgtttgtg gcgaacctgt ccggagcggt gcgggcaaca gatgttatgt tcaatcagac
     3181 tgatctgcgg gtacaaaaaa agccccggag ggcttttaac tactgactta tttttttgag
     3241 gatttgctgg gtttcaattt gtcagtattc cccggcgttt ttttattgtc acgctggcgt
     3301 ttatcaggtt cccctgttga atcggcaatt cgtttcggac ccggcttaag acccataatc
     3361 gttatccttt caatagttaa gaggaaagcc ctcaagctgg atatattgac agcgttacgg
     3421 attatcaaga ggggccagca agattatttt ttgttctttt aggttgattg cgatactatt
     3481 gctatacatg tatatcaaat ggtgttcagg aggtttatat gcttgctatc cggttatctg
     3541 atgagattga gtcccgtctg gactcgctgg cgaagcaaac cggcagaaca aagacgttct
     3601 atgcgcggga agcaatactg gcgcatctgg aagacctgga agattattat ctttcagcag
     3661 aaactgccgc acgcgttcgc cgtggtgatg aagccgtgca ttcatctgaa gacgtgagga
     3721 agtcacttgg tctggacgat taactattcc gaccgggcgc tcaaatcgtt acgcaagatg
     3781 gacaaacaga acgcacgacg gattgtggat tttatggatt tacgcattgc agttgctgcc
     3841 gatcctcgcc agtcagggaa gccgctcaaa ggtgagctgg gcgagttctg gcgctatcgg
     3901 gtgggagatt atcgcattct gtgtgagatc cgggatgacg agctggttat ccttgccgcc
     3961 accattggcc atcgccgcga agtttacgac tgaagcccgc tatgcgggct ttttttgatg
     4021 ctgacaacgg atgttatgtt taatcgccct caccggcggc gtcgcggctg ccaggtaaag
     4081 gtggtttccg ccgcctcgta tttctcccga cgtatttcct gcgcctgtct ggcttcacgt
     4141 tcgtcctgaa tggaccggag caccctttcc cgcacctgtt gttttaccgc ctttggcagc
     4201 gttctgttcc ctttcaggcc catggccagt gcctgagcga caaaggcgtc cgccaggcgc
     4261 ttttcttcag cgggagtcag tttcaggtgt gtcatggtgg cctccgggcg tctcaggttt
     4321 cctgaacagt ttaaccgcgg gtcctcccct cataccagtt agctttcaga tgtgtctata
     4381 ccacctgaaa tgtaagtgtg atgtacatct caaataataa aatttgttgc cgtgagaaat
     4441 tttagggaaa agacgcaatc tgcttacagc cgggtccggg tgcttttttg tgcctgaaga
     4501 tgagaactaa tctcatatat tgacacctga aaacggcagg agtaaggtaa taattgcact
     4561 gtatgtatat acagtatgtt gattttaatt tattgtttta aatgtcaaag aggaaaacga
     4621 tatgagtggc ggagacggtc gaggtccggg taattcaggt ctgggacata atggtggtca
     4681 ggccagtggg aatgtgaacg gtacgtctgg taaaggcggc ccttcatcag gtgggggtac
     4741 ggatccaaac agcgggccgg gctggggtac gacgcatacg cctaacggag atattcataa
     4801 ctacaatccg ggggagtttg gtaacggcgg gagtaaaccc ggtggtaatg gtggtaacag
     4861 cggcaatcat agcggtagct ctggtggcgg acagtcttcg gccactgcga tggcctttgg
     4921 tctgcctgcc ctggctactc cgggggctga aggactggct ttatcagttt ccggcgatgc
     4981 gttgtcggcc gccgttgctg atgtgctggc tgccctgaag gggccgttta agtttggtct
     5041 gtgggggatt gcgatctatg gtgtactgcc ttctgagatt gcaaaagacg atccgaaaat
     5101 gatgtcaaaa attatgacgt cattaccggc cgatgcggtg acggagactc cggcaagtac
     5161 tttaccactg gaccaggcga cggttcgtgt cagacaacgg gttgtggatg tggtgaagga
     5221 tgagcggcag catattgcgg ttgtcgcagg tcggccaatg agtgtccctg tggtggatgc
     5281 gaaaccgaca aaacgtccgg gggtattcag tgtgtcgatt ccgggtctcc cgtctctgca
     5341 ggtgagcgta ccgaaaggtg ttccgacagc gaaagccccg ccaaaaggca ttgttgctga
     5401 aaaaggtgat tcacgtccgg ctggttttac agccggtggt aactcccgtg aggccgttat
     5461 tcgtttcccg aaagagaccg gacagaagcc ggtttatgtg tcggtgacag atgttcttac
     5521 cccggcacag gtaaaacagc gtcaggagga agaaaagcgt cgccagcagg catgggacgc
     5581 cgctcatccg gaagaggggc tgaaaagaga ctatgataaa gcgaaagccg agctggatgc
     5641 cgaagataaa aatattgcga ccttaaacag ccgcattgca tcgacagaga aggcgatccc
     5701 cggtgcaagg gctgctgttc aggaagccga taaaaaggta aaagaggcag aggcaaataa
     5761 agatgatttt gtgacttata acccgcctca tgaatatggc tccgggtggc aggatcaggt
     5821 tcgctatctt gataaggata ttcagaatca gaatgagaaa ttaaaagcgg ctcaggcatc
     5881 tttaaacaca atgaatgatg ccttatccag agataaggct gcgctttccg gggcgatgga
     5941 gagccggaaa caaaaggaga aaaaagcgaa ggatgcagaa aataaattaa atgaggaaaa
     6001 gaaaaaaact cgcaagggag ctaaagatta cggccatgat tatcatccag ccccgaaaac
     6061 tgaagacata aagggactgg gtgacctcaa aaaaggtaca cctaaaacac caatgcaggg
     6121 aggtggaggt aggcgtaaac gctggattgg tgataaaggc cgtaagattt atgaatggga
     6181 ctcccagcac ggtgagcttg aagggtatcg tgccagtgat ggcgaacacc tcggggcatt
     6241 tgatcctaaa acgggtaagc aaattaaagg tccggatccg aaagggcgaa acattaaaaa
     6301 atatctttaa gaggtaagta tggggcttaa attagattta acctggtttg ataagaaaac
     6361 agaagagttt aaaggggaag agtattctaa agactttggt gatgatggtt ctgtcattga
     6421 aagtcttggg atgcctttaa aggataatat taacaatggt tgttttgatg tgaaaaatga
     6481 gtgggtttca ttattgcaac cctactttaa acataaaatc aatctttctg atagttcata
     6541 ttttgtttca tttgattatc gggatggtaa ctggtaatca aaatgtatca aggatgatgt
     6601 gaaatacaga cttccggtgt tcagggagga atgctggagt ctttaaatac agggaaagta
     6661 tatgaaaaag gcaaagacta tttttctttt tatattgatt gcttctggct ttcttctggt
     6721 tgcatgccag gcaaactata tccgggatgt tcagggcgga acggtggctc catcgtcctc
     6781 ctctgaactg acggggatcg cggttcagta gaaaagatta aaggatcttc ttgagatcct
     6841 ttttttctgc gcgtaatctt ttgccctgta a
//
