LOCUS       NZ_CP093494            11818 bp    DNA     circular CON 12-JUN-2024
DEFINITION  Klebsiella pneumoniae strain VA126 plasmid pVA126-12, complete
            sequence.
ACCESSION   NZ_CP093494
VERSION     NZ_CP093494.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26642716
            Assembly: GCF_030061795.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11818)
  AUTHORS   Marcoleta,A.E., Hormazabal,J.C. and Araya,P.
  TITLE     Complete genome sequencing and analysis of carbapenem-resistant
            Klebsiella pneumoniae isolated in Chile
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11818)
  AUTHORS   Marcoleta,A.E., Hormazabal,J.C. and Araya,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2022) Biology, Grupo de Microbiologia Integrativa,
            Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425 Nunoa,
            Santiago, RM 780000, Chile
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: unicycler v. 0.4.9b; Canu v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 181X
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030061795.1-RS_2024_06_12
            Annotation Date                   :: 06/12/2024 05:22:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,709
            CDSs (total)                      :: 5,585
            Genes (coding)                    :: 5,432
            CDSs (with protein)               :: 5,432
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 53 of 153
            Pseudo Genes (incomplete)         :: 105 of 153
            Pseudo Genes (internal stop)      :: 14 of 153
            Pseudo Genes (multiple problems)  :: 17 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093494.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11818
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="VA126"
                     /isolation_source="Isolated from blood of a 33 years old
                     male"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pVA126-12"
                     /geo_loc_name="Chile: Metropolitan Region"
                     /collection_date="2019-01-16"
                     /collected_by="Instituto de Salud Pblica de Chile"
     gene            1..957
                     /locus_tag="MO410_RS28440"
                     /old_locus_tag="MO410_28410"
     CDS             1..957
                     /locus_tag="MO410_RS28440"
                     /old_locus_tag="MO410_28410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_128502104.1"
                     /translation="MSELVVFKANELAVSRYDLTEHETKLILFCVAKLNPTIENPTRDE
                     LTVKFSCSEYARTMGLSYENAWGRLNSATSDLFKRSVELIYPTGAVSKRIFNWTEYAEF
                     NREEQTVTLVFSSYIQPLLFHLKKFIKYNLEHVKAFENKYSMRIYEWLLKELSQRKTHR
                     GNIEISIKEFKFMLMLEKNYPLYAELNRWILKPVTNDLNTYSNMKLTIEKRGRPADTLI
                     FQVELDKQIDLVTELEKDPASKKEDKTIRLTPENRLHEGLKTTLHDALTAKIQLTGFEA
                     KFLSDMQSKYDLNGSFSWLTQKQRATLEKILAKYGRI"
                     /name="replication initiation protein"
     gene            965..1390
                     /locus_tag="MO410_RS28445"
                     /old_locus_tag="MO410_28415"
     CDS             965..1390
                     /locus_tag="MO410_RS28445"
                     /old_locus_tag="MO410_28415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_097470724.1"
                     /translation="MALVSVTEAARLTGKNRTTIHRYIKQGKLSQISDATNTKKVDTSE
                     LIRVFGSLTATHATLSQSDAMQHDATPKTDQSVALLQQKIALLEQLLENKNKELQRQDE
                     HIDSLKQAMLLIESKLPTTPEPVTPVPAKKSWQFWKK"
                     /name="helix-turn-helix domain-containing protein"
     gene            1784..2758
                     /locus_tag="MO410_RS28450"
                     /old_locus_tag="MO410_28420"
     CDS             1784..2758
                     /locus_tag="MO410_RS28450"
                     /old_locus_tag="MO410_28420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128502106.1"
                     /translation="MLKKSSVALALSLALSGYASAAEAPIVDSKTDLGTLRPGNGGYSY
                     ASGVSADGTVVVGESRTDSGDYNAFVWREGDTKMTGLGTLKADNSGYSSASGVSADGTV
                     VVGYSDTDEGDTHATVWKVKYPVPPVEPPVVTIVDATNSSKAMADTGRRGFKVLDLYQS
                     SLNSLSQSRCQLGQSDYCVGVFSQYDTVSSNHRLATGLFGAIRLPAENWTVGGAMNFAN
                     GTQLIDNYDTRGSNQPGIGVFTRYQENRDNTGLSAELSGVVIRCSRENELVRYSSNSCP
                     ECSIRWHVVDAVGQDIRPLEKTFHNACQESYWLKVNRQTHLPV"
                     /name="hypothetical protein"
     gene            complement(2767..3411)
                     /locus_tag="MO410_RS28455"
                     /old_locus_tag="MO410_28425"
     CDS             complement(2767..3411)
                     /locus_tag="MO410_RS28455"
                     /old_locus_tag="MO410_28425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     gene            complement(3505..>3654)
                     /locus_tag="MO410_RS28460"
                     /old_locus_tag="MO410_28430"
                     /pseudo=""
     CDS             complement(3505..>3654)
                     /locus_tag="MO410_RS28460"
                     /old_locus_tag="MO410_28430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3744..4328)
                     /locus_tag="MO410_RS28465"
                     /old_locus_tag="MO410_28435"
     CDS             complement(3744..4328)
                     /locus_tag="MO410_RS28465"
                     /old_locus_tag="MO410_28435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA modification GTPase"
                     /protein_id="WP_163621336.1"
                     /translation="MSDEIKQIAALIGHQQAQEKRVAALIDEFQAESAKLSRQTAQLAQ
                     VIHELDSASGKMTDTIRQSVNAALTQVEKELKQTGLAQQKPAVNALEQVVTAANASVSA
                     MRREMSRYTWKSAIYIVLTMLAVMGSCLWGMVCFIDSGYDRIAKMERMEAVWQKKAPLA
                     DISTCDGKPCVKVDTSQSYGDKKDTYLIIKQ"
                     /name="tRNA modification GTPase"
     gene            complement(4341..5456)
                     /gene="mobA"
                     /locus_tag="MO410_RS28470"
                     /old_locus_tag="MO410_28440"
     CDS             complement(4341..5456)
                     /gene="mobA"
                     /locus_tag="MO410_RS28470"
                     /old_locus_tag="MO410_28440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_097470721.1"
                     /translation="MASYHLSVKTGGKGKAASHADYIAREGKYAREKDSDLEHKESGNM
                     PAWAAHKPAEFWKAADTFERANGCTYREIEIALPRELKPEQRLELVRDFIRQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERMNDGIDRDPQQYFKRANSKEPERGGAKKVRFG
                     ETPTERREYLIAQRERWATLQNNHLERYQHPDRVDARSLKAQGIDREPERHLGAGQVQR
                     FDTDQLQAILERRDAERQVQQCCDERDSVIDVTTSLREALIERDTLTPKQAQQPEPEQD
                     AVSGRVFDFEKEPDKLNELVSDAMKDIQEEIDLQSLVNDAMAEFQGIHQEMVRQQERTA
                     LAEKQRQQEKERQRIAEQKQQKPDKGWSFSR"
                     /name="mobA"
     gene            5719..5808
                     /locus_tag="MO410_RS28475"
                     /old_locus_tag="MO410_28445"
     CDS             5719..5808
                     /locus_tag="MO410_RS28475"
                     /old_locus_tag="MO410_28445"
                     /inference="COORDINATES: protein motif:HMM:NF044726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_283557650.1"
                     /translation="MNRWILKPVTNDLNTYSNMKLTIEKRSSC"
                     /name="hypothetical protein"
     gene            6116..6541
                     /locus_tag="MO410_RS28480"
                     /old_locus_tag="MO410_28450"
     CDS             6116..6541
                     /locus_tag="MO410_RS28480"
                     /old_locus_tag="MO410_28450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_097470724.1"
                     /translation="MALVSVTEAARLTGKNRTTIHRYIKQGKLSQISDATNTKKVDTSE
                     LIRVFGSLTATHATLSQSDAMQHDATPKTDQSVALLQQKIALLEQLLENKNKELQRQDE
                     HIDSLKQAMLLIESKLPTTPEPVTPVPAKKSWQFWKK"
                     /name="helix-turn-helix domain-containing protein"
     gene            7167..7586
                     /locus_tag="MO410_RS28485"
                     /old_locus_tag="MO410_28455"
     CDS             7167..7586
                     /locus_tag="MO410_RS28485"
                     /old_locus_tag="MO410_28455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_283557648.1"
                     /translation="MTGLGTLKADNSGYSSASGVSADGTVVVGYSDTDEGDTHATVWKV
                     KYPVPPVEPPVVTIVDATNSSKAMADTGRRGFKVLDLYQSSLNSLSQSRCQLGQSDYCV
                     GVFSQYDTVSSNHRLATGLFGAIRLPAENWTVGGR"
                     /name="hypothetical protein"
     gene            7583..7906
                     /locus_tag="MO410_RS28490"
                     /old_locus_tag="MO410_28460"
     CDS             7583..7906
                     /locus_tag="MO410_RS28490"
                     /old_locus_tag="MO410_28460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_283557649.1"
                     /translation="MNFANGTQLIDNYDTRGSNQPGIGVFTRYQENRDNTGLSAELSGV
                     VIRCSRENELVRYSSNSCPECSIRWHVVDAVGQDIRPLEKTFHNACQESYWLKVNRQTH
                     LPV"
                     /name="hypothetical protein"
     gene            complement(7915..8558)
                     /locus_tag="MO410_RS28495"
                     /old_locus_tag="MO410_28465"
                     /pseudo=""
     CDS             complement(7915..8558)
                     /locus_tag="MO410_RS28495"
                     /old_locus_tag="MO410_28465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="QnrB family quinolone resistance pentapeptide
                     repeat protein"
                     /name="QnrB family quinolone resistance pentapeptide repeat
                     protein"
     gene            complement(8652..>8800)
                     /locus_tag="MO410_RS28500"
                     /old_locus_tag="MO410_28470"
                     /pseudo=""
     CDS             complement(8652..>8800)
                     /locus_tag="MO410_RS28500"
                     /old_locus_tag="MO410_28470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(8890..9474)
                     /locus_tag="MO410_RS28505"
                     /old_locus_tag="MO410_28475"
     CDS             complement(8890..9474)
                     /locus_tag="MO410_RS28505"
                     /old_locus_tag="MO410_28475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA modification GTPase"
                     /protein_id="WP_163621336.1"
                     /translation="MSDEIKQIAALIGHQQAQEKRVAALIDEFQAESAKLSRQTAQLAQ
                     VIHELDSASGKMTDTIRQSVNAALTQVEKELKQTGLAQQKPAVNALEQVVTAANASVSA
                     MRREMSRYTWKSAIYIVLTMLAVMGSCLWGMVCFIDSGYDRIAKMERMEAVWQKKAPLA
                     DISTCDGKPCVKVDTSQSYGDKKDTYLIIKQ"
                     /name="tRNA modification GTPase"
     gene            complement(9487..10602)
                     /gene="mobA"
                     /locus_tag="MO410_RS28510"
                     /old_locus_tag="MO410_28480"
     CDS             complement(9487..10602)
                     /gene="mobA"
                     /locus_tag="MO410_RS28510"
                     /old_locus_tag="MO410_28480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_097470721.1"
                     /translation="MASYHLSVKTGGKGKAASHADYIAREGKYAREKDSDLEHKESGNM
                     PAWAAHKPAEFWKAADTFERANGCTYREIEIALPRELKPEQRLELVRDFIRQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERMNDGIDRDPQQYFKRANSKEPERGGAKKVRFG
                     ETPTERREYLIAQRERWATLQNNHLERYQHPDRVDARSLKAQGIDREPERHLGAGQVQR
                     FDTDQLQAILERRDAERQVQQCCDERDSVIDVTTSLREALIERDTLTPKQAQQPEPEQD
                     AVSGRVFDFEKEPDKLNELVSDAMKDIQEEIDLQSLVNDAMAEFQGIHQEMVRQQERTA
                     LAEKQRQQEKERQRIAEQKQQKPDKGWSFSR"
                     /name="mobA"
     gene            10768..11223
                     /locus_tag="MO410_RS28515"
                     /old_locus_tag="MO410_28485"
     CDS             10768..11223
                     /locus_tag="MO410_RS28515"
                     /old_locus_tag="MO410_28485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_097470722.1"
                     /translation="MKNEKQAKWLTDRVQYIRGLKAPNEQQRLLVILHDKTDKTAQEIK
                     TLSLLIQAERAAEKAQEARTKVMNLIHAEKRAEAKAARKARDHALYQSAGLLIMAGLVD
                     SQTGKPVDDPAKLLGALASLHELSRDNPRWEEWKLKGQVLLNQKKED"
                     /name="conjugal transfer protein TraD"
     ARG             complement(7919..8535)
                     /name="qnrB"
                     /gene="qnrB"
                     /gene_name="QnrB family quinolone resistance pentapeptide
                     repeat protein"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="96.26"
     ARG             complement(2771..3411)
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(7916..8382)
                     /name="QnrB19"
                     /gene="QnrB19"
                     /gene_name="quinolone resistance protein (qnr)"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic"
                     /identity="100.0"
                     /coverage="72.43"
     Typing          2..957
                     /name="rep_cluster_1155"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.97"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5585..6108
                     /name="rep_cluster_1155"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4345..5456)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.367"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(9491..10602)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.367"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtctgaat tagttgtgtt taaagcaaat gaattagcag taagccgtta tgatctaact
       61 gaacatgaaa ccaagctaat tctgttttgc gttgcaaagt tgaaccctac aattgaaaac
      121 ccaacaaggg atgaattaac agttaaattc tcatgttctg aatatgcgcg cactatgggc
      181 ttaagttatg aaaatgcttg gggaagattg aacagtgcaa ctagtgattt atttaaacgc
      241 tctgttgaat taatttatcc tacaggagca gtttctaaac gaattttcaa ttggacggaa
      301 tacgctgagt tcaatagaga agaacaaact gtaacattag tatttagctc atatattcaa
      361 ccacttttat ttcatttaaa aaaattcatt aaatacaacc ttgagcatgt taaagccttt
      421 gaaaataaat actcaatgcg aatatatgaa tggttactaa aggagctttc acaaagaaaa
      481 acgcataggg gaaacattga gataagtatc aaggaattta agtttatgct catgcttgaa
      541 aaaaactacc ctttatatgc agaattgaat cgctggatcc taaaaccagt tacaaatgac
      601 ttaaacactt acagcaatat gaagttgact attgaaaaac gcggtcgtcc tgctgacaca
      661 ctgatctttc aagttgaact ggataagcaa attgaccttg tgactgaact agaaaaagat
      721 ccagcatcaa aaaaagaaga taagacaatc cgtttaacac ctgaaaatcg tcttcatgag
      781 gggctaaaaa caacattgca tgatgcttta actgcaaaaa ttcagcttac cggctttgaa
      841 gctaaatttc tgagtgacat gcagagcaaa tatgatctaa acggctcgtt ctcatggctg
      901 actcaaaaac aacgagccac gctagagaaa atattggcaa aatacggacg gatctgaggt
      961 atctatggca ctggtatcag ttacagaggc agccagacta actggaaaaa acagaacaac
     1021 aatacatcgc tatattaagc aaggaaaact gtcgcaaatt tctgatgcaa caaacacaaa
     1081 aaaagtagat acatcagagc ttattcgtgt attcggctca ctaacggcga cacatgcaac
     1141 actgtcgcaa agcgatgcaa tgcagcacga tgcaacacct aaaacagatc aatccgttgc
     1201 actgttacaa caaaaaattg cacttttaga gcaactgctt gaaaacaaaa ataaagaatt
     1261 acaacgtcag gacgagcata tagacagcct gaaacaagca atgctgttaa tcgaatccaa
     1321 gctgccgaca acacctgaac ccgttacacc agtaccagcc aaaaaatcgt ggcaattctg
     1381 gaaaaaatag ccggttggcg gcattttcac cctgaaaccg aaatcgtcat tttgataaca
     1441 aattggcaac ataaaaatcg ggctttttac agggagaaaa cagggcattt tgcgcggttg
     1501 agaaaaaatt caggagcaaa gcctaaacgg aagtgcgctt aagcaccctt atgttaaatc
     1561 tgagcaatag cagaacggta atcatttaat ttataacaaa aaaaaaaaaa taaatatttg
     1621 tttgtttata tcaaaatact ttaaaatttt attttaaacc ccccgccact atagccaacc
     1681 aggatgacct ctacccggac catcgctgcc cccacaccgc agcggtacaa ataaaaaatt
     1741 ttattttgtc acgtaagtga cttctttttg gagcgataac aatatgttaa aaaaaagcag
     1801 tgtagccctg gcattaagcc tggcattaag tgggtatgcc tccgcggcag aagcccccat
     1861 tgtggacagt aaaactgacc tgggaacttt acgcccgggg aatggagggt attcctatgc
     1921 cagtggggtc tcggcggacg gcacagtagt agtgggagag tccaggactg acagcggcga
     1981 ctataatgcc tttgtgtggc gtgagggaga caccaaaatg accggtctcg gcacccttaa
     2041 agccgacaac agcgggtatt cctctgccag tggggtctcg gcggacggga ctgtagtagt
     2101 cgggtactca gatactgatg aaggtgatac ccatgccacc gtgtggaaag tgaaataccc
     2161 ggtgccgccg gtagagccgc cggtagtaac gatagtcgat gcaaccaatt catccaaagc
     2221 gatggcggat accggtcgtc gcggttttaa agtgttagac ctgtaccaga gcagcttaaa
     2281 cagcttaagt cagagccgtt gtcagttagg ccagagtgat tattgcgtcg gggttttcag
     2341 ccagtatgat accgtcagca gtaatcaccg tctggcaacc ggtctgttcg gggcaatccg
     2401 tttaccggcc gaaaactgga cggtgggcgg ggcgatgaac ttcgccaacg gcactcagct
     2461 tattgataac tacgacacgc gcggcagcaa tcagccgggc atcggggtat tcacccgtta
     2521 tcaggaaaac cgcgataata cgggtctgag tgccgagtta tcgggagtgg ttattcgttg
     2581 ttctcgcgaa aatgaattgg ttagatattc atcaaattcc tgtccagaat gcagcattag
     2641 atggcatgtc gttgatgcgg taggtcagga tattcgtcca ctggagaaaa cgtttcacaa
     2701 tgcttgccag gagtcgtact ggttaaaagt aaatcgtcaa acgcatctcc cggtgtaaac
     2761 gctgaactaa ccaatcacag cgatgccaag ccgctccatg agcaacgatg cctggtagct
     2821 gtctaactta acgccttgta aatcaacacc ccgaatatct aagtcaccta actccgaatt
     2881 ggtcagatcg caatgtgtga agtttgctgc tcgccagtcg aaagtcgaaa actcgccgcc
     2941 ggagagatct gaaccactga acgtcgcacc cagtacctga gtccccatcc agcggttttc
     3001 ccacagctca cacttttcca acacgacttt cgaaaaattg gcgtagctta gattagtatt
     3061 agtgatatat gcgctgcaaa accaggtgcg cgtggtgatc atattcataa agcttgcacc
     3121 gcggaaatct gcgccttgtg cgcggcagtg gcgaatttca atgcccaagg cactgacgtt
     3181 gcggaaatct gccattgata aatcacagct tttgaaaatg gcatctttca gcattgcgcg
     3241 actaaaattg caccctttct gactttcgcg atcatagaac tggcagccga taaattcagt
     3301 gccgctcagg tcggcacctg aaaaatcgca gttaaaaaat gtactatttt caactttctc
     3361 accggtgaag cgatttctgt caattttttc gccaactaat gccagagtca tatctatgcc
     3421 tgtgttttta tacagtaatg acgccatggt aaaccttatg acgttatgcg tcaaacccgc
     3481 cagtatgaca cctgcaaatg cgtattagat ctggtgtttc ttcagcaagg cacgtagctg
     3541 atgataagtc agacccagta attcagcggc ttttttctgg ttaaatttag cctgctgtaa
     3601 gctggtttgt agaaagtctt tctcttgctg ctgctggaat tcacgcagat ccagcgtaaa
     3661 tggcttttat aatccacgcc gaagacattc aacactcggc tggaaatcgc cggtggcatt
     3721 tgaggcatat tcagcgccgt tttttattgt ttgataatca gatacgtgtc ttttttatcg
     3781 ccatatgact gactggtatc cactttcacg caaggttttc catcacacgt ggagatatca
     3841 gccagcgggg cttttttctg ccagaccgct tccatgcgct ccatttttgc aattcggtca
     3901 tagccactgt cgataaaaca gaccatcccc caaagacagc tccccatgac cgcaagcatc
     3961 gtcagaacaa tataaattgc agacttccac gtatagcgcg acatctcacg tcgcattgca
     4021 ctcacagacg cattagccgc tgtaaccacc tgttccagcg cattgacggc gggtttctgc
     4081 tgcgccaatc ccgtttgttt gagttctttt tcaacctgcg ttaaagcggc attcaccgat
     4141 tgcctgatcg tgtcggtcat cttaccggag gcactgtcca gttcatgaat tacctgcgcc
     4201 aattgtgctg tctggcggct taatttggct gattccgcct gaaattcatc aatcagcgcg
     4261 gctacgcgtt tttcctgtgc ctgctgatgc ccgataaggg cagcaatctg tttaatctcg
     4321 tcgctcatgt aaaatcctgt ctatcgtgaa aatgaccagc ctttgtctgg tttttgttgt
     4381 ttctgttcgg caatacgctg ccgttctttc tcctgctgtc tttgcttttc agcaagcgcg
     4441 gtgcgttcct gctgcctgac catctcctga tgtatgccct gaaactctgc catcgcatcg
     4501 ttaacaagtg actgaaggtc gatttcttct tgtatgtcct tcatggcatc gctgaccagt
     4561 tcattcagct tgtcaggctc tttttcaaaa tcgaacaccc tgccggaaac ggcgtcctgt
     4621 tcaggctccg gctgctgcgc ctgttttggc gtcagagtgt cccgctcaat aagggcttcc
     4681 cgtaacgaag tggtcacgtc aatcacgctg tcacgttcat cacagcactg ctgaacctgt
     4741 ctttcggcat ccctgcgttc aagaatggcc tgtaactggt cagtatcgaa gcgttgaacc
     4801 tgtcccgcgc ccagatgccg ctccggctca cgatcgatac cctgtgcttt cagggaacgc
     4861 gcatccacac ggtcaggatg ctgataacgc tcaagatggt tattttgtaa tgttgcccag
     4921 cgttcccgct gcgcgataag gtattccctg cgttcggtcg gcgtttcgcc gaaccgcacc
     4981 tttttcgcgc cgccacgctc aggctcttta ctgttcgccc gtttaaaata ctgctgcgga
     5041 tcacggtcaa tgccgtcatt catccgttca gagaacatga tatgcgcatg cggctgttcg
     5101 ccaccggcta tcgctgcttt cgggttatgg atagcaaact gatacgcatg acggtcgcca
     5161 atttcctgcc ggataaaatc gcgaaccagc tcaagccgct gctcaggttt cagctctcgt
     5221 ggcagggcga tttcaatctc gcggtaggtg cagccgttgg cacgttcaaa cgtgtcagcg
     5281 gctttccaga actcagccgg tttatgcgct gcccatgcgg gcatattgcc ggactcctta
     5341 tgctcaaggt cggagtcttt ttcacgggca tattttccct cacgcgcaat ataatcggca
     5401 tgggaagcgg ctttgccttt cccgccggtt ttgacgctga ggtgataaga ggccatagtg
     5461 ttttcgcccg ccgcaggcgt gcaccgtttc tgaacatagt gaagaaacgt ctaagtgcgc
     5521 actgtcaata aaaaggatga cagggattgt agtggaattt gacctgagtt gccaataata
     5581 ggcataatta ttccattcga aaatatgaat ggttactaaa ggagctttca caaagaaaaa
     5641 cgcataggga aacattgaga taagtatcaa ggaatttaag tttatgctca tgaaaaaaac
     5701 taccctttat atgcagaatt gaatcgctgg atcctaaaac cagttacaaa tgacttaaac
     5761 acttacagca atatgaagtt gactattgaa aaacggtcgt cctgctgaca cactgatctt
     5821 tcaagttgaa ctggataagc aaattgacct tgtgactgaa ctagaaaaag atccagcatc
     5881 aaaaaagaag ataagacaat ccgtttaaca cctgaaaatc gtcttcatga ggggctaaaa
     5941 acaacattgc atgatgcttt aactgcaaaa attcagctta ccggctttga agctaaattt
     6001 ctgagtgaca tgcagagcaa atatgatcta aacggctcgt tctcatggct gactcaaaaa
     6061 caacgagcca cgctagagaa aatattggca aaatacggac ggatctgagg tatctatggc
     6121 actggtatca gttacagagg cagccagact aactggaaaa aacagaacaa caatacatcg
     6181 ctatattaag caaggaaaac tgtcgcaaat ttctgatgca acaaacacaa aaaaagtaga
     6241 tacatcagag cttattcgtg tattcggctc actaacggcg acacatgcaa cactgtcgca
     6301 aagcgatgca atgcagcacg atgcaacacc taaaacagat caatccgttg cactgttaca
     6361 acaaaaaatt gcacttttag agcaactgct tgaaaacaaa aataaagaat tacaacgtca
     6421 ggacgagcat atagacagcc tgaaacaagc aatgctgtta atcgaatcca agctgccgac
     6481 aacacctgaa cccgttacac cagtaccagc caaaaaatcg tggcaattct ggaaaaaata
     6541 gccggttggc ggcattttca ccctgaaacc gaaatcgtca ttttgataac aaattggcaa
     6601 cataaaaatc gggcttttta cagggagaaa acagggcatt ttgcgcggtt gagaaaaaat
     6661 tcaggagcaa agcctaaacg gaagtgcgct taagcaccct tatgttaaat ctgagcaata
     6721 gcagaacggt aatcatttaa tttataacaa aaaaaaaaat aaatatttgt ttgtttatat
     6781 caaaatactt taaaatttta ttttaaaccc cccgccacta tagccaacca ggatgacctc
     6841 tacccggacc atcgctgccc ccacaccgca gcggtacaaa taaaaaattt tattttgtca
     6901 cgtaagtgac ttctttttgg agcgataaca atatgttaaa aaaaagcagt gtagccctgg
     6961 cattaagcct ggcattaagt gggtatgcct ccgcggcaga agcccccatt gtggacagta
     7021 aaactgacct gggaacttta cgcccgggga atggagggta ttcctatgcc agtggggtct
     7081 cggcggacgg cacagtagta gtgggagagt ccaggactga cagcggcgac tataatgcct
     7141 ttgtgtggcg tgagggagac accaaaatga ccggtctcgg cacccttaaa gccgacaaca
     7201 gcgggtattc ctctgccagt ggggtctcgg cggacgggac tgtagtagtc gggtactcag
     7261 atactgatga aggtgatacc catgccaccg tgtggaaagt gaaatacccg gtgccgccgg
     7321 tagagccgcc ggtagtaacg atagtcgatg caaccaattc atccaaagcg atggcggata
     7381 ccggtcgtcg cggttttaaa gtgttagacc tgtaccagag cagcttaaac agcttaagtc
     7441 agagccgttg tcagttaggc cagagtgatt attgcgtcgg ggttttcagc cagtatgata
     7501 ccgtcagcag taatcaccgt ctggcaaccg gtctgttcgg ggcaatccgt ttaccggccg
     7561 aaaactggac ggtgggcggg cgatgaactt cgccaacggc actcagctta ttgataacta
     7621 cgacacgcgc ggcagcaatc agccgggcat cggggtattc acccgttatc aggaaaaccg
     7681 cgataatacg ggtctgagtg ccgagttatc gggagtggtt attcgttgtt ctcgcgaaaa
     7741 tgaattggtt agatattcat caaattcctg tccagaatgc agcattagat ggcatgtcgt
     7801 tgatgcggta ggtcaggata ttcgtccact ggagaaaacg tttcacaatg cttgccagga
     7861 gtcgtactgg ttaaaagtaa atcgtcaaac gcatctcccg gtgtaaacgc tgaactaacc
     7921 aatcacagcg atgccaagcc gctccatgag caacgatgcc tggtagctgt ctaacttaac
     7981 gccttgtaaa tcaacacccc gaatatctaa gtcacctaac tccgaattgg tcagatcgca
     8041 atgtgtgaag tttgctgctc gccagtcgaa agtcgaaaac tcgccgccgg agagatctga
     8101 accactgaac gtcgcaccca gtacctgagt ccccatccag cggttttccc acagctcaca
     8161 cttttccaac acgactttcg aaaaattggc gtagcttaga ttagtattag tgatatatgc
     8221 gctgcaaaac caggtgcgcg tggtgatcat attcataaag cttgcaccgc ggaaatctgc
     8281 gccttgtgcg cggcagtggc gaatttcaat gcccaaggca ctgacgttgc ggaaatctgc
     8341 cattgataaa tcacagcttt tgaaaatggc atctttcagc attgcgcgac taaaattgca
     8401 ccctttctga ctttcgcgat catagaactg gcagccgata aattcagtgc cgctcaggtc
     8461 ggcacctgaa aaatcgcagt taaaaaatgt actattttca actttctcac cggtgaagcg
     8521 atttctgtca atttttcgcc aactaatgcc agagtcatat ctatgcctgt gtttttatac
     8581 agtaatgacg ccatggtaaa ccttatgacg ttatgcgtca aacccgccag tatgacacct
     8641 gcaaatgcgt attagatctg gtgtttcttc agcaaggcac gtagctgatg ataagtcaga
     8701 cccagtaatt cagcggcttt ttctggttaa atttagcctg ctgtaagctg gtttgtagaa
     8761 agtctttctc ttgctgctgc tggaattcac gcagatccag cgtaaatggc ttttataatc
     8821 cacgccgaag acattcaaca ctcggctgga aatcgccggt ggcatttgag gcatattcag
     8881 cgccgttttt tattgtttga taatcagata cgtgtctttt ttatcgccat atgactgact
     8941 ggtatccact ttcacgcaag gttttccatc acacgtggag atatcagcca gcggggcttt
     9001 tttctgccag accgcttcca tgcgctccat ttttgcaatt cggtcatagc cactgtcgat
     9061 aaaacagacc atcccccaaa gacagctccc catgaccgca agcatcgtca gaacaatata
     9121 aattgcagac ttccacgtat agcgcgacat ctcacgtcgc attgcactca cagacgcatt
     9181 agccgctgta accacctgtt ccagcgcatt gacggcgggt ttctgctgcg ccaatcccgt
     9241 ttgtttgagt tctttttcaa cctgcgttaa agcggcattc accgattgcc tgatcgtgtc
     9301 ggtcatctta ccggaggcac tgtccagttc atgaattacc tgcgccaatt gtgctgtctg
     9361 gcggcttaat ttggctgatt ccgcctgaaa ttcatcaatc agcgcggcta cgcgtttttc
     9421 ctgtgcctgc tgatgcccga taagggcagc aatctgttta atctcgtcgc tcatgtaaaa
     9481 tcctgtctat cgtgaaaatg accagccttt gtctggtttt tgttgtttct gttcggcaat
     9541 acgctgccgt tctttctcct gctgtctttg cttttcagca agcgcggtgc gttcctgctg
     9601 cctgaccatc tcctgatgta tgccctgaaa ctctgccatc gcatcgttaa caagtgactg
     9661 aaggtcgatt tcttcttgta tgtccttcat ggcatcgctg accagttcat tcagcttgtc
     9721 aggctctttt tcaaaatcga acaccctgcc ggaaacggcg tcctgttcag gctccggctg
     9781 ctgcgcctgt tttggcgtca gagtgtcccg ctcaataagg gcttcccgta acgaagtggt
     9841 cacgtcaatc acgctgtcac gttcatcaca gcactgctga acctgtcttt cggcatccct
     9901 gcgttcaaga atggcctgta actggtcagt atcgaagcgt tgaacctgtc ccgcgcccag
     9961 atgccgctcc ggctcacgat cgataccctg tgctttcagg gaacgcgcat ccacacggtc
    10021 aggatgctga taacgctcaa gatggttatt ttgtaatgtt gcccagcgtt cccgctgcgc
    10081 gataaggtat tccctgcgtt cggtcggcgt ttcgccgaac cgcacctttt tcgcgccgcc
    10141 acgctcaggc tctttactgt tcgcccgttt aaaatactgc tgcggatcac ggtcaatgcc
    10201 gtcattcatc cgttcagaga acatgatatg cgcatgcggc tgttcgccac cggctatcgc
    10261 tgctttcggg ttatggatag caaactgata cgcatgacgg tcgccaattt cctgccggat
    10321 aaaatcgcga accagctcaa gccgctgctc aggtttcagc tctcgtggca gggcgatttc
    10381 aatctcgcgg taggtgcagc cgttggcacg ttcaaacgtg tcagcggctt tccagaactc
    10441 agccggttta tgcgctgccc atgcgggcat attgccggac tccttatgct caaggtcgga
    10501 gtctttttca cgggcatatt ttccctcacg cgcaatataa tcggcatggg aagcggcttt
    10561 gcctttcccg ccggttttga cgctgaggtg ataagaggcc atagtgtttt cgcccgccgc
    10621 aggcgcgcac cgtttctgaa catagtgaag aaacgtctaa gtgcgcactg tcaataaaaa
    10681 ggatgacagg gattgtagtg gaatttgacc tgagttgcca ataatgagca taattattcc
    10741 attcgaaaaa cagcatgagt ggtaaagatg aaaaacgaaa aacaggctaa gtggctcaca
    10801 gacagagtgc agtatatccg tggtttaaaa gcaccgaatg aacaacaacg tttattggtg
    10861 atattgcacg ataaaacgga taaaaccgct caggaaataa agacgctctc attgttaatt
    10921 caggcagagc gtgcagcaga aaaagcacag gaagcccgca cgaaagtgat gaacctgata
    10981 cacgctgaga aacgagcaga agccaaagcc gcccgtaaag cgcgtgacca tgccctttat
    11041 caatcggcgg gattactgat tatggcaggg ttagtggaca gtcagacggg taaaccggtg
    11101 gatgaccccg ccaaactact gggcgcatta gccagcttgc atgaattatc ccgtgataat
    11161 cccaggtggg aagaatggaa attgaagggg caggtattac tcaatcagaa aaaggaagat
    11221 tagaacaggg tgttaatacc ctctaaaacg ccacaggaag tccgtggcga ggttttacag
    11281 gctcggtaat ggatttcatc gcctaacact gaaagcgtgg cacagaagcg catacagcca
    11341 ctttcctgat tgagccgtga gcgaagcgaa ctcaatgaca cggaaaaaac agcggcgcag
    11401 tggctggatg gtcggagaca aaagaaaggg tcagcgagtt gcccgggcaa agcgagggag
    11461 ctactgaggt gttttaggtt tgttttgaaa aggagaaaac cttgtttgcg acatcctttt
    11521 aactacggca aatcgacggc agttttctga gttatccaca gggctgggat ctattctttt
    11581 tttatcttta ttattctttc tttattctag tgattttaaa tcattgtttt ttaataaaaa
    11641 aaaaatcatt aaatgtaggg aaaattaggc tggatgtagg aaaaattagg ctagcagtgg
    11701 gaaagatgta ggaaaaatta ggttattcac aatcaaacat aaaaaatgta gttgatttta
    11761 attgctacat tattatgatt tatgtggcga tttaaattac tacataatgg tgattggc
//
