LOCUS       NZ_CP093479             6871 bp    DNA     circular CON 12-JUN-2024
DEFINITION  Klebsiella pneumoniae strain VA569 plasmid pVA569-7, complete
            sequence.
ACCESSION   NZ_CP093479
VERSION     NZ_CP093479.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26642719
            Assembly: GCF_030061715.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6871)
  AUTHORS   Marcoleta,A.E., Hormazabal,J.C. and Araya,P.
  TITLE     Complete genome sequencing and analysis of carbapenem-resistant
            Klebsiella pneumoniae isolated in Chile
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6871)
  AUTHORS   Marcoleta,A.E., Hormazabal,J.C. and Araya,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2022) Biology, Grupo de Microbiologia Integrativa,
            Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425 Nunoa,
            Santiago, RM 780000, Chile
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: unicycler v. 0.4.9b; Canu v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 164X
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030061715.1-RS_2024_06_12
            Annotation Date                   :: 06/12/2024 05:22:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,710
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 45 of 129
            Pseudo Genes (incomplete)         :: 81 of 129
            Pseudo Genes (internal stop)      :: 17 of 129
            Pseudo Genes (multiple problems)  :: 13 of 129
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093479.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6871
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="VA569"
                     /isolation_source="Isolated from peritoneal fluid of a 79
                     years old male"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pVA569-7"
                     /geo_loc_name="Chile: Metropolitan Region"
                     /collection_date="2019-04-06"
                     /collected_by="Instituto de Salud Pblica de Chile"
     gene            join(6711..6871,1..85)
                     /locus_tag="MO417_RS28430"
                     /old_locus_tag="MO417_28405"
     CDS             join(6711..6871,1..85)
                     /locus_tag="MO417_RS28430"
                     /old_locus_tag="MO417_28405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080090623.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNRTLPKAVKQQVRERV
                     LRSIQDEREARQAQEIRREKYEAAETTFTWQPRRRR"
                     /name="hypothetical protein"
     gene            complement(142..408)
                     /locus_tag="MO417_RS28435"
                     /old_locus_tag="MO417_28410"
     CDS             complement(142..408)
                     /locus_tag="MO417_RS28435"
                     /old_locus_tag="MO417_28410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000262326.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRILCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(392..616)
                     /locus_tag="MO417_RS28440"
                     /old_locus_tag="MO417_28415"
     CDS             complement(392..616)
                     /locus_tag="MO417_RS28440"
                     /old_locus_tag="MO417_28415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(1464..1676)
                     /locus_tag="MO417_RS28445"
                     /old_locus_tag="MO417_28420"
     CDS             complement(1464..1676)
                     /locus_tag="MO417_RS28445"
                     /old_locus_tag="MO417_28420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1680..2165)
                     /locus_tag="MO417_RS28450"
                     /old_locus_tag="MO417_28425"
     CDS             complement(1680..2165)
                     /locus_tag="MO417_RS28450"
                     /old_locus_tag="MO417_28425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_013631087.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(<2203..2880)
                     /locus_tag="MO417_RS28570"
                     /pseudo=""
     CDS             complement(<2203..2880)
                     /locus_tag="MO417_RS28570"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2843..3163)
                     /locus_tag="MO417_RS28460"
                     /old_locus_tag="MO417_28435"
     CDS             complement(2843..3163)
                     /locus_tag="MO417_RS28460"
                     /old_locus_tag="MO417_28435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_045265547.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLETGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3855..3989
                     /locus_tag="MO417_RS28465"
                     /old_locus_tag="MO417_28440"
     CDS             3855..3989
                     /locus_tag="MO417_RS28465"
                     /old_locus_tag="MO417_28440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_165451377.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPEKHGKTPLAAATGKQIEQEIC"
                     /name="replication initiation protein"
     gene            complement(4195..4344)
                     /locus_tag="MO417_RS28470"
                     /old_locus_tag="MO417_28445"
     CDS             complement(4195..4344)
                     /locus_tag="MO417_RS28470"
                     /old_locus_tag="MO417_28445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_080090625.1"
                     /translation="MKKAKTIFLFILIASGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(4429..4686)
                     /locus_tag="MO417_RS28475"
                     /old_locus_tag="MO417_28450"
     CDS             complement(4429..4686)
                     /locus_tag="MO417_RS28475"
                     /old_locus_tag="MO417_28450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_022644819.1"
                     /translation="MGLKLDLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGCFDVKNEWVSLLQPYFKHKINLSDSSYFVSFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(4696..6384)
                     /locus_tag="MO417_RS28480"
                     /old_locus_tag="MO417_28455"
     CDS             complement(4696..6384)
                     /locus_tag="MO417_RS28480"
                     /old_locus_tag="MO417_28455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_080090624.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNTMNDALSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKTRKGAKDYGHDYHPAPKTEDIKGLGDLKKGTPKTPMQGGGGRRKRWIGDKGRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQIKGPDPKGRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             1..6871
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(3271..3358)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          3381..3924
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.091"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1349..2853)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.618"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agccagacag gcgcaggaaa tacgtcggga gaaatacgag gcggcggaaa ccacctttac
       61 ctggcagccg cgacgccgcc ggtgagggcg attaaacata acatccgttg tcagcatcaa
      121 aaaaagcccg catagcgggc ttcagtcgta aacttcgcgg cgatggccaa tggtggcggc
      181 aaggataacc agctcgtcat cccggatctc acacagaatg cgataatctc ccacccgata
      241 gcgccagaac tcgcccagct cacctttgag cggcttccct gactggcgag gatcggcagc
      301 aactgcaatg cgtaaatcca taaaatccac aatccgtcgt gcgttctgtt tgtccatctt
      361 gcgtaacgat ttgagcgccc ggtcggaata gttaatcgtc cagaccaagt gacttcctca
      421 cgtcttcaga tgaatgcacg gcttcatcac cacggcgaac gcgtgcggca gtttctgctg
      481 aaagataata atcttccagg tcttccagat gcgccagtat tgcttcccgc gcatagaacg
      541 tctttgttct gccggtttgc ttcgccagcg agtccagacg ggactcaatc tcatcagata
      601 accggatagc aagcatataa acctcctgaa caccatttga tatacatgta tagcaatagt
      661 atcgcaatca acctaaaaga acaaaaaata atcttgctgg cccctcttga taatccgtaa
      721 cgctgtcaat atatccagct tgagggcttt cctcttaact attgaaagga taacgattat
      781 gggtcttaag ccgggtccga aacgaattgc cgattcaaca ggggaacctg ataaacgcca
      841 gcgtgacaat aaaaaaacgc cggggaatac tgacaaattg aaacccagca aatcctcaaa
      901 aaaataagtc agtagttaaa agccctccgg ggcttttttt gtacccgcag atcagtctga
      961 ttgaacataa catctgttgc ccgcaccgct ccggacaggt tcgccacaaa cggggcggca
     1021 gtcaggtaaa accggcaaca aaccacccga tacaccgccc cgttcgtgac agataaattt
     1081 tacccggctg catggtcatg cagccagatg catacggtct gatttcaggc cagaatccgg
     1141 atgaacgcct taaaaacagg aaaatgcttc tccagccagt cccagatctc cagcgccatg
     1201 tacaggcagt tcagcaccgt ctcaatcacg ttaaacagtc tcataatccc tccgttgtat
     1261 acgctgtgat ggaaacatca cgaccgtatt tagcgggaaa tttatgagcc cttcttcttg
     1321 gggtttgtca ctggatgcgc gcagtcagcc cagcgtcgga ccgttatagc tccgctgccg
     1381 ggaacgggtt tcagccagct ctttttcatg ctgtttctgc tcgtatgccc gttcggcggc
     1441 caccgccatt tcatgccgct gcactactgg ctcaaacgct gtaactgcct gctcaagttc
     1501 gccacctgct gactcaagcg cgtgacgcgc tcgttcagcg ccgcgttctc ccgttgcata
     1561 agaccagaca tcttctgcca ttccgcgaag gcgctctccc actcgtccag cctttgcgag
     1621 tagtcctgat gtaactgctc taatgcggtc agcaactgtt tttccagttc cgtcatgggc
     1681 tatttcctct ccagtaccat ccagttccct tcctccccgt aagctcccgc tttcgggttc
     1741 actttgacgc aggacagttt cttctccccg cagagcgaca gctgcacgcc gtggttcttc
     1801 tccgacagca tggcctgcgt ccgctcctgc gcccggatag tctggtaatt cccggtgatc
     1861 tgctgcccct gccaccacag aatgctcccg ctcgtggcga tcagcagtac cgagaccagg
     1921 gtgatggtca gccacgtccg gccaaccata cgcagcacgc tcagccggtt cgactgcatc
     1981 gccgcggtca taccctgctc gtgggcgctg atggcgtcgc tgattctctt cgcgcttgcg
     2041 ctcagttccg cttttacaga ctgctcgtgc tcgctgaatg cggctttcag catctcgccg
     2101 gtactctgct gctgcgcttt cgattgctgc tccaagtcct tcgccagcgc taaaagactg
     2161 ctcataaatg gctcccttca gtcggatgtt tcgccccccg tccgggtcgg caatgctgat
     2221 actgtttttt gtggtgcgga ccacctcaaa acctgccgct gtgagcgccc ctgtgacgtc
     2281 ctcgcgcgtt ttgagctccc cggatgaggc taaggccagt aaaccccgtg taatcgcctg
     2341 agcggcttcc agcttcgttt ccggcagtgc ggacggcgtg accagtaacc gccggttctc
     2401 cggggcgttt ggatcatgca gccccagtct gccgttcacc acggtctgcc aggcatcgat
     2461 gcgcggccgg tcggcgcgat cgtagtacgg ctgcaggcgt ttcccggtca gcagttcagt
     2521 gttggggatc aggaaattca gctccagccg ccccttgtcg gtgtgctcca cccacagaat
     2581 gctgtactgg tctttatcga gcccgggcat cagaacccgc tcaaagctgg ccatgatctg
     2641 ctcccgctgc ccgggcggca gttcagcctc cgcgaacgac agcacaccgg aggtgtactt
     2701 tttcgcatac ggcgaggcat cgataagctc ccggacttcc tccggctttc cctgcagcac
     2761 ggtcgccccc tcgcgctgcc ggtcttttcc cagcaggtaa tccaccggac cggcaccacc
     2821 accacgcccc cggggatgga atttaacgat catcatcccc ccctgtttcc agcaccgcat
     2881 gccgcagccg ctccagtccc gcgtcgattg ccatcagggc ggccacgatc tgcacccgat
     2941 catgaccggc accaccggca ttcactttcc gggcgatctg gttcaggttg ttacccatac
     3001 cggcaagctg gcgcagcagc gccggcgata tcgacggcag tctgccggca cgtgcaggct
     3061 tctcatcgag gcaggtctga cgcatccacg ccgccagctg cttgccgtcg caccgctcga
     3121 gcagccgccg gtgttcgtct tccgtcaccc acatcgtgag catcttgttg cgtttatctg
     3181 ccagagtagc ctcctgataa caccgggacg ggcgggaaat ttttcccggg cggcacggtg
     3241 ttggacaagc cgcaggcgcg tcagtcgctt ttagcgggtt tcggggcgca gccctgaacc
     3301 agtcacgtag cgctagcgga gtgtatactg gcttaattat gcggcatcag tgcggattgt
     3361 atgaaaagtg caccatgacc ggtgtgaaat accgcacaga tgcgtaagga gaacatgcag
     3421 gtaccggtgc tcttccgctt cctcgctcac tggctcgctg cgctcggtcg ttcggctgcg
     3481 gcgagcggtg tctgctcact caaaagcggc gatacggtta tccacagaat caggggataa
     3541 cgccggaaag agctgtgtgc aacaagacaa agcccgggaa aaaggtcgcg ccggaggcgc
     3601 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3661 tggcgaaacc cgacaggact taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3721 gctctcctgt tccgaccctg ccacttaccg gatacctgtc cgcctttctc ccttcgggaa
     3781 gcgtggcgct ttctcatagc tcacgctgtt ggtatctcgg cttggtgtag gtcgttcgct
     3841 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3901 actatcgtct cgagtccaac ccggaaaagc acggtaaaac gccactggca gcagccactg
     3961 gtaagcagat tgagcaagag atatgttgag agttcttgaa gtggtggcct aactgcggct
     4021 acactggaag gacagttttg gtgacacgtc tcttgctcag actgttaccc ggttaagcag
     4081 ttccccaact gacttatcct tcgatcaaac cgcctcccca ggcggttttt tcgtttacag
     4141 ggcaaaagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttaatc ttttctactg
     4201 aaccgcgatc cccgtcagtt cagaggagga cgatggagcc accgttccgc cctgaacatc
     4261 ccggatatag tttgcctggc atgcaaccag aagaaagcca gaagcaatca atataaaaag
     4321 aaaaatagtc tttgcctttt tcatatactt tccctgtatt taaagactcc agcattcctc
     4381 cctgaacacc ggaagtctgt atttcacatc atccttgata cattttgatt accagttacc
     4441 atcccgataa tcaaatgaaa caaaatatga actatcagaa agattgattt tatgtttaaa
     4501 gtagggttgc aataatgaaa cccactcatt tttcacatca aaacaaccat tgttaatatt
     4561 atcctttaaa ggcatcccaa gactttcaat gacagaacca tcatcaccaa agtctttaga
     4621 atactcttcc cctttaaact cttctgtttt cttatcaaac caggttaaat ctaatttaag
     4681 ccccatactt acctcttaaa gatatttttt aatgtttcgc cctttcggat ccggaccttt
     4741 aatttgctta cccgttttag gatcaaatgc cccgaggtgt tcgccatcac tggcacgata
     4801 cccttcaagc tcaccgtgct gggagtccca ttcataaatc ttacggcctt tatcaccaat
     4861 ccagcgttta cgcctacctc cacctccctg cattggtgtt ttaggtgtac cttttttgag
     4921 gtcacccagt ccctttatgt cttcagtttt cggggctgga tgataatcat ggccgtaatc
     4981 tttagctccc ttgcgagttt ttttcttttc ctcatttaat ttattttctg catccttcgc
     5041 ttttttctcc ttttgtttcc ggctctccat cgccccggaa agcgcagcct tatctctgga
     5101 taaggcatca ttcattgtgt ttaaagatgc ctgagccgct tttaatttct cattctgatt
     5161 ctgaatatcc ttatcaagat agcgaacctg atcctgccac ccggagccat attcatgagg
     5221 cgggttataa gtcacaaaat catctttatt tgcctctgcc tcttttacct ttttatcggc
     5281 ttcctgaaca gcagcccttg caccggggat cgccttctct gtcgatgcaa tgcggctgtt
     5341 taaggtcgca atatttttat cttcggcatc cagctcggct ttcgctttat catagtctct
     5401 tttcagcccc tcttccggat gagcggcgtc ccatgcctgc tggcgacgct tttcttcctc
     5461 ctgacgctgt tttacctgtg ccggggtaag aacatctgtc accgacacat aaaccggctt
     5521 ctgtccggtc tctttcggga aacgaataac ggcctcacgg gagttaccac cggctgtaaa
     5581 accagccgga cgtgaatcac ctttttcagc aacaatgcct tttggcgggg ctttcgctgt
     5641 cggaacacct ttcggtacgc tcacctgcag agacgggaga cccggaatcg acacactgaa
     5701 tacccccgga cgttttgtcg gtttcgcatc caccacaggg acactcattg gccgacctgc
     5761 gacaaccgca atatgctgcc gctcatcctt caccacatcc acaacccgtt gtctgacacg
     5821 aaccgtcgcc tggtccagtg gtaaagtact tgccggagtc tccgtcaccg catcggccgg
     5881 taatgacgtc ataatttttg acatcatttt cggatcgtct tttgcaatct cagaaggcag
     5941 tacaccatag atcgcaatcc cccacagacc aaacttaaac ggccccttca gggcagccag
     6001 cacatcagca acggcggccg acaacgcatc gccggaaact gataaagcca gtccttcagc
     6061 ccccggagta gccagggcag gcagaccaaa ggccatcgca gtggccgaag actgtccgcc
     6121 accagagcta ccgctatgat tgccgctgtt accaccatta ccaccgggtt tactcccgcc
     6181 gttaccaaac tcccccggat tgtagttatg aatatctccg ttaggcgtat gcgtcgtacc
     6241 ccagcccggc ccgctgtttg gatccgtacc cccacctgat gaagggccgc ctttaccaga
     6301 cgtaccgttc acattcccac tggcctgacc accattatgt cccagacctg aattacccgg
     6361 acctcgaccg tctccgccac tcatatcgtt ttcctctttg acatttaaaa caataaatta
     6421 aaatcaacat actgtatata catacagtgc aattattacc ttactcctgc cgttttcagg
     6481 tgtcaatata tgagattagt tctcatcttc aggcacaaaa aagcacccgg acccggctgt
     6541 aagcagattg cgtcttttcc ctaaaatttc tcacggcaac aaattttatt atttgagatg
     6601 tacatcacac ttacatttca ggtggtatag acacatctga aagctaactg gtatgagggg
     6661 aggacccgcg gttaaactgt tcaggaaacc tgagacgccc ggaggccacc atgacacacc
     6721 tgaaactgac tcccgctgaa gaaaagcgcc tggcggacgc ctttgtcgct caggcactgg
     6781 ccatgggcct gaaagggaac agaacgctgc caaaggcggt aaaacaacag gtgcgggaaa
     6841 gggtgctccg gtccattcag gacgaacgtg a
//
