LOCUS       NZ_CP093475             7218 bp    DNA     circular CON 12-JUN-2024
DEFINITION  Klebsiella pneumoniae strain VA591 plasmid pVA591-7, complete
            sequence.
ACCESSION   NZ_CP093475
VERSION     NZ_CP093475.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26642720
            Assembly: GCF_030061685.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Marcoleta,A.E., Hormazabal,J.C. and Araya,P.
  TITLE     Complete genome sequencing and analysis of carbapenem-resistant
            Klebsiella pneumoniae isolated in Chile
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7218)
  AUTHORS   Marcoleta,A.E., Hormazabal,J.C. and Araya,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2022) Biology, Grupo de Microbiologia Integrativa,
            Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425 Nunoa,
            Santiago, RM 780000, Chile
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: unicycler v. 0.4.9b; Canu v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 180X
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030061685.1-RS_2024_06_12
            Annotation Date                   :: 06/12/2024 05:21:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,517
            CDSs (total)                      :: 5,396
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 60 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 19 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093475.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="VA591"
                     /isolation_source="Isolated from blood of a 29 years old
                     male"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pVA591-7"
                     /geo_loc_name="Chile: Metropolitan Region"
                     /collection_date="2019-04-11"
                     /collected_by="Instituto de Salud Pblica de Chile"
     gene            join(7169..7218,1..2848)
                     /locus_tag="MO415_RS27490"
                     /old_locus_tag="MO415_27450"
     CDS             join(7169..7218,1..2848)
                     /locus_tag="MO415_RS27490"
                     /old_locus_tag="MO415_27450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_000091108.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPNSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            complement(3416..>3757)
                     /locus_tag="MO415_RS27495"
                     /old_locus_tag="MO415_27455"
                     /pseudo=""
     CDS             complement(3416..>3757)
                     /locus_tag="MO415_RS27495"
                     /old_locus_tag="MO415_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            <3756..4358
                     /locus_tag="MO415_RS27500"
                     /old_locus_tag="MO415_27460"
                     /pseudo=""
     CDS             <3756..4358
                     /locus_tag="MO415_RS27500"
                     /old_locus_tag="MO415_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="klebicin B-related nuclease bacteriocin"
                     /name="klebicin B-related nuclease bacteriocin"
     gene            4360..4617
                     /locus_tag="MO415_RS27505"
                     /old_locus_tag="MO415_27465"
     CDS             4360..4617
                     /locus_tag="MO415_RS27505"
                     /old_locus_tag="MO415_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_025714833.1"
                     /translation="MAGNKISDFTEEEFTAFVSKIIAADFPTDKENDDAIYSFAQLTEH
                     PAGWNLIFKPKVGEDSSAKGIVKTVKEWRAANGKPGFKKA"
                     /name="bacteriocin immunity protein"
     gene            4725..4880
                     /locus_tag="MO415_RS27510"
                     /old_locus_tag="MO415_27470"
     CDS             4725..4880
                     /locus_tag="MO415_RS27510"
                     /old_locus_tag="MO415_27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323621.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071561715.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     gene            complement(5038..5199)
                     /locus_tag="MO415_RS27600"
     CDS             complement(5038..5199)
                     /locus_tag="MO415_RS27600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049165871.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPERHDKAPLAAATGNGFRGVGLE
                     VMRLLRLN"
                     /name="hypothetical protein"
     gene            5604..5795
                     /locus_tag="MO415_RS27515"
                     /old_locus_tag="MO415_27475"
     CDS             5604..5795
                     /locus_tag="MO415_RS27515"
                     /old_locus_tag="MO415_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5795..6217
                     /locus_tag="MO415_RS27520"
                     /old_locus_tag="MO415_27480"
     CDS             5795..6217
                     /locus_tag="MO415_RS27520"
                     /old_locus_tag="MO415_27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714834.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(6469..7074)
                     /locus_tag="MO415_RS27525"
                     /old_locus_tag="MO415_27485"
     CDS             complement(6469..7074)
                     /locus_tag="MO415_RS27525"
                     /old_locus_tag="MO415_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     Typing          complement(5087..5556)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.834"
                     /coverage="92.0"
                     /evalue="2.73e-170"
ORIGIN
        1 tctgcccacg gacgaactgt cgctactgaa gttctacacg ctgggcgatg atgacctgga
       61 aaacattagg cagcgccgca gaccggaaaa caggattggc tttgccctgc aactttgtgc
      121 cttacgatat ccgggccgtg cactggctcc tggtgagatg atcccgcgtg aagtcctttc
      181 cttcgtcggt gctcagcttg gagttccggc tgatgcgctt ctcacttatg ccacacggcg
      241 ccaaacccgt cagcagcaca tggacacgct gcgcgaaatt tacggctaca agaccttcac
      301 gggccgtggt gcccgtgatc tgcgtgagtg gactttcggc caggccgaag atgccagatc
      361 aaacgaggat cttgctcatc gttttattgt gcggtgtcgg gaaacttcca ccattctgcc
      421 cgcagtatcg acaatcgagc gcttgtgcgc ggatgctctg gtcgccgctg agcggcggat
      481 tgaaacgcgg attgcggaaa atttaacagc ggatgttcgc gatcacctgg acaaacttct
      541 gagtgaaatg ctcgccggca atatcagtcg tttcatctgg cttcgcaact tcgaggttgg
      601 taacaactcg gctgctgcta accgtttgct cgacaggctc gaatttctgc gtaccctgaa
      661 tatcaatcat agtgctttgg ccagcatacc tgcccatcgc attgcccggc tgcgtcggca
      721 gggtgaacgc tacttcaccg acggtttgcg tgacatcact tcggaccgcc gctgggcgat
      781 ccttgccgtc tgtgttgtgg agtgggaagc ggcgattgct gatgccatag tcgaaaccca
      841 tgacaggatc gtaggaaaaa cctggcggga agcgaagcgc cagcatgacg aaacaatttc
      901 cggctctaaa gccacactca cggatacgat ccgtaccttc accgcgctgg gagcttcgtt
      961 gcttgaggcc cgcagtgacg gaaccccgct ggagatggct gtcgccagtt cggttgcatg
     1021 ggaccggctc gctcaactgg tagcgacagg gactcaactc agcaacacgc tagccgatga
     1081 gcctcttgca tatgtcgggc agggatacca tcgctttcgt cgttatgcgc cccgcatgtt
     1141 gcgctgtctg aagctcgaag ccgcgccggt cgccggacca ttggtagcag cagctttgtc
     1201 gatcggagag atgaaaggtg ttgcatcgcc agaaaggcgt ttcctgcggc ccaactccaa
     1261 atggaaccgt catttacgag ctcaggaaaa aggagatacc cgtctttggg aagtggcggt
     1321 actctttcac ctccgggatg cttttcgttc cggagatgtc tggctcgctc attcgcgccg
     1381 ctatggtgac ctcaagcagg tactggtgcc gatgatcgcg gcgcaggaaa atgcaaaact
     1441 ggccgtgcct tccaacccac aggattggct ggcagacaga aaggcgcgac tcacgatcgc
     1501 tcttaagcgg ctggcccggg ctgcccgtaa cggcactatt ccgcacggta gcatagaaga
     1561 tggaacgttg cggatcgaca ggttgacagc agacgtgccg gatggtgccg aggcactcat
     1621 actggatctg tatcgccgaa tgccgtccgt tcggattacc gacatgctgc ttgaagttga
     1681 tgcagccctt ggtttcacag atgcgtttac ccatctgaga accggggctc catgtcgcga
     1741 ccggatcggt ctgctcaacg tcctgctcgc tgaagggctc aatctgggcc tgcgtaagat
     1801 ggcggaagct acaaacacgc atgattactg gcagctctca cgccttgccc gctggcatgt
     1861 tgaaagcgaa gccatgaacc aggcattggc aattgtggtg gccgcgcagg gtaaactgcc
     1921 gatgtcacgc gtctggggga tgggcacgtc agcatcgagc gatggtcagt ttttcccgac
     1981 agcgcggcat ggcgaagcca tgaacatggt caatgccaaa tatggttctg ttcccggcct
     2041 caaagcgtat actcacgtaa gcgaccagtt cgcgccattc gcttgtcagt cgatcccggc
     2101 gaccgtgagc gaggcaccgt atattctcga tggactactg atgaacgagg tcggtcgcca
     2161 tgttcgcgaa cagtatgccg atacagcagg attcaccgac catttgttcg gagccagtag
     2221 cctgctcggc tacaatctcg ttctgcgaat cagggatctg ccatcgaagc ggttgtacgt
     2281 atttaatccc gatacgaccc ccagggagtt acgcaagttg gtaggtggaa aagcccggga
     2341 ggatcttatc gttgcgaact ggcctgatat tttccgttgt gccgcgacga tgaccgctgg
     2401 caaaatcagg cccagccaac tcctgcgcaa gctcgcttct tacccacgac aaaacaacct
     2461 tgcagttgcg cttcgtgaag ttggtcgtat tgaacggacc cttttcatta ttgagtggat
     2521 cctggatacg gacatgcagc ggcgtgctca gatcggtctt aacaagggag aggcccacca
     2581 tgcgctcaaa aatgcgctcc gtatcgggag gcagggggaa attcgcgatc gcacgacaga
     2641 ggggcagcac taccgaatcg ctgggctcaa tttattgact gcggtgatca tttactggaa
     2701 taccgtccat cttggtcatg ccgtcacgga gcggcggaac gaagggttgg atgttccccc
     2761 tgaatttctt ccccacatat ccccattggg ctgggcgcac attctactga ctggcgaata
     2821 tctttggccc aaggaaccga aagcttaggg tgtcatttcg ccctcagccg gaaccgaccc
     2881 ctctatggcg aatcgtttac gaattgccca cgattcatat gtgaatcacg ctttattatc
     2941 atgttgttat gatgaatcga ctcttgattc aattcatcaa tttgggcgtt ttttgttcat
     3001 ggtgactctt gattcaattc acgattcgaa tcgccggtga atcgtgtggt gagtcgtccg
     3061 gcgacttgcc ataacgtgat tgccggccgt cgacgaaccc gattcctgaa cgtgctgaaa
     3121 accggttttc cggctgcgcc ggcaacgcct cacggccacg caataaattg cgtccccgtt
     3181 caccgttttc ggatcagaag aacgccggaa acaggactta ccctggctga atggcgtgaa
     3241 tttccgcgct atgcacttgc gcgcatactc atgcatgccg taaaaacaga gcctgcgcgt
     3301 ttctggcggg ttttcgggtg gtttgttgcc tgttttaccg gtttcccgtc agaaacgtcc
     3361 tgaggccgtt ttagcggtgc gtacaattaa gggattatgg taaatggtga cttggttaat
     3421 cataaacatc ctgggggatg gcatacacca gttggatatc cggtgcatcg ttgagccatc
     3481 gacctgattt tttcattaag tagccaccaa tttcttcggt agtcagccgg gcatgaaaca
     3541 atgcttcatc attgaaccag cagtcagggt gatagatgag atccagtggg tttgccttga
     3601 aattaatctc cagtaactgg agtgcataac tgtggtcaga tgcactacct tctgctttgc
     3661 gtatgaagtt gataacgtcg ttcagttctt tccatgtaaa gtcatcgagg tatttttcct
     3721 gattaagcgc catcctggtg aaatctagtg cacttgacgc acctggtgca aacggaccac
     3781 tgacgctaag cgggccggta ccactaccgg agcgtattct gcataccggt gatcagattt
     3841 cggcgccaca ggctacagat aaaaccgtga cgccggtagc ggatgacctc gattttgacg
     3901 atattattct ggtgtttcct ccggaatccg gcctaaaacc gctgtatgtg atgtaccgta
     3961 gccctcgtaa catgcccggt acagtgagcg gcaaagggca gaacgttggc aataactgga
     4021 tggggggggc cagtaccggg gatggtgctc ctgttccttc ccagattgcg gataaattac
     4081 gcgggaagac tttcggtagt tttgattctt tccgtcgggc gttctggaaa gcggttgctg
     4141 acgattctgc actgagcaag caatttagtg aagctgatat taatcagatg aaagctggaa
     4201 gggcacctac tgcggacttt ttagaatcag tagggaaacg tgttaaaatt gaattacacc
     4261 acgaaaaaga aatatctcaa ggaggggctg ttatggatgt cgataacatt aaggcgctaa
     4321 cacctaaaaa tcatattgag acacataaag gaaaataaaa tggctggtaa taaaatttca
     4381 gattttacgg aagaagaatt tactgctttt gttagtaaaa taatcgctgc tgattttcca
     4441 actgacaaag agaacgatga tgctatttat tcgttcgccc aactaacaga gcatcctgct
     4501 ggttggaacc tcatcttcaa gcctaaggta ggcgaagatt cttcggcgaa aggaattgtt
     4561 aaaaccgtaa aggaatggcg agctgctaac ggtaagccag gttttaaaaa agcgtgattg
     4621 aatttgcggg ttcaagttat gagcccgctc atttgtaact ctggatttga agaataatta
     4681 ctagatatcc atttgcctag aataataaaa ataaaaggtg atatatgaaa aaattacttt
     4741 tcctaggggt tgttaccgtt tctgtggttc tggcagcatg tcaggtcaat aatgttcgag
     4801 acaccggagg tggggctgtt tcaccttcat cgacggtgac cggagtcacg cttgatagcg
     4861 gtacgaacgg caatccgtaa aggatcttct tgagatccca tttggatcgt cgtaatctct
     4921 tgctctgtaa acgaaaaaac cgccttgcag ggcggttttt ccgaaggttc aaacacttgc
     4981 aggtgattga accaaggtaa ctggcttaga ggagcgcagt caccaaaact tgttctttca
     5041 gtttagcctt aacaggcgca tgacttcaag accaactcct ctaaatccgt taccagtggc
     5101 tgctgccagt ggcgctttgt cgtgtctttc cgggttggac tcaagatgat agttaccgga
     5161 taagacgcag cagtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
     5221 tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat aacagcggaa
     5281 tgacaccggt aaaccgaatg gcaggaacag gagagcgcac gagggagcca tcagggggaa
     5341 acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag cgtcaaattc
     5401 tgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ccgctgtgct gccttctccc
     5461 tctccgagct gtctgctcta tgtcgttatg tgcgcgttgc gaccgctcgc cgcagccgaa
     5521 cggcaggagc gtagcgactg agtgagcgag gaagcggaaa agagatctgt ctgacattga
     5581 gcactgacgc tctgaggccc tttatgacga aacaacagaa aacagcgctg aacatggcta
     5641 aattcatcca gaatcagtct ctgctcctgc tggagaagct taacgagctt gatctcgatg
     5701 ttgaggccga cctttgtgag aaactccacg atgatgcgga gcatcttttc cgtacactgt
     5761 cctcgagact ggatgaatta caggatggca attgatggcc agagtgtcta ttagtgaagc
     5821 ggcacggctt gttaaggtta gccgtcccac aatttacaag atgattaaca gtgggaagtt
     5881 aagttacacc tctgttgtaa agcacgggaa ggctattaaa gttattgata cttcagagct
     5941 tattagagtt tttggttctc ttgatggtgt catcgatact gtaaaatatg atgtaaaaag
     6001 tgacgcagaa tctacaagta ttaacagcgt tggtttacat gatttacaac atagaatcgc
     6061 tctacttgaa gctgaaaacg atggtcttaa aggggctgtt aaggctcgag atgagcatat
     6121 tgattccctt cgtcaggcta tgcagcttct ggagcacaag cgcgagcctt cttcaccacc
     6181 ccattccccg tggtggaaat tctggaaaaa atcctgatcc tgccatgctt gagttttcct
     6241 ctccggccat cccagggagt tcctgaacct tcccaaaaat cgccagatct ctgaacgctc
     6301 ctcacgacta aaaataccca cacactacgc aaaattaaaa ttttgcagat tccttattta
     6361 aaacaattgg ttaatgaaaa attaatgtgc agtgatgtgt tacaaagtgc agatccaagt
     6421 cgtggacgac tctatggggt cggttccggc tgagggcgaa atgacaccct aagcgttagc
     6481 tctgtgtcgt tgcacgatgt cagcgacggt attcttgctg ataccgagct cgcgtgcgat
     6541 ccagcgatag ctgcgtccct cggccctcat cgcaaccacc ttaggcaaaa gtcggtctga
     6601 ttttggtcgc actccggcct gacgaccaag cctcttacca cgtgccttcg caacagcaag
     6661 gcctgacttg acccgctcgc tgatgagatc ccgctcaaac tccgcaatgc cggaaagaaa
     6721 cgtcgccagc attcgtccat acggcgacga aagatcgaac gccattccat tcatggctat
     6781 cacggaaacc ttccagttct ccagttcacg tagcgtattg agcagatcga gcgtcgagcg
     6841 cccccaccgg gaaagctcag tgaccaggat tgcatcaatt tgtctggact gggcaagcgc
     6901 caggactttc tttcgctcgg cccggtcgag tttagttcct gaacctgttt ccttaaatat
     6961 tcccaccacg tcgtagccgg cacggccggc gaaggctcgc agatcaaatt cctggcgttc
     7021 acaagactga tccgctgttg aaacccggca gtaaatggcg gcacgatgtc ccaattgaac
     7081 cctcctggat ttttgtatcg gaacgccctg atttatatgg gctggctgtt gtccaaaaca
     7141 gactatactt caaaagggac gaatttgtat gtcacgacgc catattttca ccgaacggca
     7201 gcgagcagcg ctgttcga
//
