LOCUS       NZ_CP093474             9391 bp    DNA     circular CON 12-JUN-2024
DEFINITION  Klebsiella pneumoniae strain VA591 plasmid pVA591-9, complete
            sequence.
ACCESSION   NZ_CP093474
VERSION     NZ_CP093474.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26642720
            Assembly: GCF_030061685.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9391)
  AUTHORS   Marcoleta,A.E., Hormazabal,J.C. and Araya,P.
  TITLE     Complete genome sequencing and analysis of carbapenem-resistant
            Klebsiella pneumoniae isolated in Chile
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9391)
  AUTHORS   Marcoleta,A.E., Hormazabal,J.C. and Araya,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2022) Biology, Grupo de Microbiologia Integrativa,
            Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425 Nunoa,
            Santiago, RM 780000, Chile
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: unicycler v. 0.4.9b; Canu v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 180X
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030061685.1-RS_2024_06_12
            Annotation Date                   :: 06/12/2024 05:21:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,517
            CDSs (total)                      :: 5,396
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 60 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 19 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093474.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9391
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="VA591"
                     /isolation_source="Isolated from blood of a 29 years old
                     male"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pVA591-9"
                     /geo_loc_name="Chile: Metropolitan Region"
                     /collection_date="2019-04-11"
                     /collected_by="Instituto de Salud Pblica de Chile"
     gene            join(8996..9391,1..6)
                     /locus_tag="MO415_RS27440"
                     /old_locus_tag="MO415_27400"
     CDS             join(8996..9391,1..6)
                     /locus_tag="MO415_RS27440"
                     /old_locus_tag="MO415_27400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_022644821.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHLSGQVSEQRDTASADQNEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(385..990)
                     /locus_tag="MO415_RS27445"
                     /old_locus_tag="MO415_27405"
     CDS             complement(385..990)
                     /locus_tag="MO415_RS27445"
                     /old_locus_tag="MO415_27405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            1085..3982
                     /locus_tag="MO415_RS27450"
                     /old_locus_tag="MO415_27410"
     CDS             1085..3982
                     /locus_tag="MO415_RS27450"
                     /old_locus_tag="MO415_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_000091108.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPNSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            complement(4414..4659)
                     /locus_tag="MO415_RS27455"
                     /old_locus_tag="MO415_27415"
     CDS             complement(4414..4659)
                     /locus_tag="MO415_RS27455"
                     /old_locus_tag="MO415_27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023284667.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKSLPKAVKQQVRERV
                     LRAIQDERESRLAKKMRREKYEAAEDTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            4986..6671
                     /locus_tag="MO415_RS27460"
                     /old_locus_tag="MO415_27420"
     CDS             4986..6671
                     /locus_tag="MO415_RS27460"
                     /old_locus_tag="MO415_27420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_101972327.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPNMMSK
                     IMTSLPADTVTETPVSTLPLEQATVRVRQRVVDVVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKREYDKAKADLDA
                     EDKNIATLNGRITSTEKAIPGARTAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALSGAMETRKQKEKKAKDAENKLNE
                     EKEKSRKGTKDYGHDYHPAPKTEDIKGLGDLKKGTPKTPMQGGGGRRKRWIGDKGRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            6681..6938
                     /locus_tag="MO415_RS27465"
                     /old_locus_tag="MO415_27425"
     CDS             6681..6938
                     /locus_tag="MO415_RS27465"
                     /old_locus_tag="MO415_27425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_022644819.1"
                     /translation="MGLKLDLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGCFDVKNEWVSLLQPYFKHKINLSDSSYFVSFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            7023..7172
                     /locus_tag="MO415_RS27470"
                     /old_locus_tag="MO415_27430"
     CDS             7023..7172
                     /locus_tag="MO415_RS27470"
                     /old_locus_tag="MO415_27430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_029497523.1"
                     /translation="MKKVKAIFLFILIVSGFLIVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(7398..7511)
                     /locus_tag="MO415_RS27475"
                     /old_locus_tag="MO415_27435"
     CDS             complement(7398..7511)
                     /locus_tag="MO415_RS27475"
                     /old_locus_tag="MO415_27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_223178531.1"
                     /translation="MQEPPFQPYCCACSGNCSLGSNPERHDKSPLAAATGN"
                     /name="replication initiation protein"
     gene            8176..8325
                     /locus_tag="MO415_RS27480"
                     /old_locus_tag="MO415_27440"
     CDS             8176..8325
                     /locus_tag="MO415_RS27480"
                     /old_locus_tag="MO415_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_165383652.1"
                     /translation="MPSEYDEQRDATDTQLPGEYDLFDLMAQVTEENLHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            8805..8996
                     /locus_tag="MO415_RS27485"
                     /old_locus_tag="MO415_27445"
     CDS             8805..8996
                     /locus_tag="MO415_RS27485"
                     /old_locus_tag="MO415_27445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_022644820.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLNASQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     BGC             1..9391
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7964..8183
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="81.696"
                     /coverage="100.0"
                     /evalue="2.7300000000000002e-42"
     Typing          7410..7595
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.617"
                     /coverage="99.0"
                     /evalue="6.160000000000001e-73"
ORIGIN
        1 tcctgatcct gccatggttg agttttcctc tccggccatt ccggggagtt cctgaacctt
       61 cccagatatc gccagatccg ggaacactcc tcaccacgaa aaatacccac acaccacgca
      121 aaattaaaat tttgcagata actccttatt atcagttggt taggttttat taaattacgc
      181 tgtattacct tctgtaaggt aaatccaagt cgtcggcgat tcgcgtacga cttgccgacg
      241 actcgtcata gagtcatcga cgactcactc catactatca gtaacttatg acgacttgac
      301 tctagagttg agtcgttcat ttggtgtttt tctgaccttg gtgactctag aggggtcggt
      361 tccggctgag ggcgaaatga caccctaagc gttagctctg tgtcgttgca cgatgtcagc
      421 gacggtattc ttgctgatac cgagctcgcg tgcgatccag cgatagctgc gtccctcggc
      481 cctcatcgca accaccttag gcaaaagtcg gtctgatttt ggtcgcactc cggcctgacg
      541 accaagcctc ttaccacgtg ccttcgcaac agcaaggcct gacttgaccc gctcgctgat
      601 gagatcccgc tcaaactccg caatgccgga aagaaacgtc gccagcattc gtccatacgg
      661 cgacgaaaga tcgaacgcca ttccattcat ggctatcacg gaaaccttcc agttctccag
      721 ttcacgtagc gtattgagca gatcgagcgt cgagcgcccc caccgggaaa gctcagtgac
      781 caggattgca tcaatttgtc tggactgggc aagcgccagg actttctttc gctcggcccg
      841 gtcgagttta gttcctgaac ctgtttcctt aaatattccc accacgtcgt agccggcacg
      901 gccggcgaag gctcgcagat caaattcctg gcgttcacaa gactgatccg ctgttgaaac
      961 ccggcagtaa atggcggcac gatgtcccaa ttgaaccctc ctggattttt gtatcggaac
     1021 gccctgattt atatgggctg gctgttgtcc aaaacagact atacttcaaa agggacgaat
     1081 ttgtatgtca cgacgccata ttttcaccga acggcagcga gcagcgctgt tcgatctgcc
     1141 cacggacgaa ctgtcgctac tgaagttcta cacgctgggc gatgatgacc tggaaaacat
     1201 taggcagcgc cgcagaccgg aaaacaggat tggctttgcc ctgcaacttt gtgccttacg
     1261 atatccgggc cgtgcactgg ctcctggtga gatgatcccg cgtgaagtcc tttccttcgt
     1321 cggtgctcag cttggagttc cggctgatgc gcttctcact tatgccacac ggcgccaaac
     1381 ccgtcagcag cacatggaca cgctgcgcga aatttacggc tacaagacct tcacgggccg
     1441 tggtgcccgt gatctgcgtg agtggacttt cggccaggcc gaagatgcca gatcaaacga
     1501 ggatcttgct catcgtttta ttgtgcggtg tcgggaaact tccaccattc tgcccgcagt
     1561 atcgacaatc gagcgcttgt gcgcggatgc tctggtcgcc gctgagcggc ggattgaaac
     1621 gcggattgcg gaaaatttaa cagcggatgt tcgcgatcac ctggacaaac ttctgagtga
     1681 aatgctcgcc ggcaatatca gtcgtttcat ctggcttcgc aacttcgagg ttggtaacaa
     1741 ctcggctgct gctaaccgtt tgctcgacag gctcgaattt ctgcgtaccc tgaatatcaa
     1801 tcatagtgct ttggccagca tacctgccca tcgcattgcc cggctgcgtc ggcagggtga
     1861 acgctacttc accgacggtt tgcgtgacat cacttcggac cgccgctggg cgatccttgc
     1921 cgtctgtgtt gtggagtggg aagcggcgat tgctgatgcc atagtcgaaa cccatgacag
     1981 gatcgtagga aaaacctggc gggaagcgaa gcgccagcat gacgaaacaa tttccggctc
     2041 taaagccaca ctcacggata cgatccgtac cttcaccgcg ctgggagctt cgttgcttga
     2101 ggcccgcagt gacggaaccc cgctggagat ggctgtcgcc agttcggttg catgggaccg
     2161 gctcgctcaa ctggtagcga cagggactca actcagcaac acgctagccg atgagcctct
     2221 tgcatatgtc gggcagggat accatcgctt tcgtcgttat gcgccccgca tgttgcgctg
     2281 tctgaagctc gaagccgcgc cggtcgccgg accattggta gcagcagctt tgtcgatcgg
     2341 agagatgaaa ggtgttgcat cgccagaaag gcgtttcctg cggcccaact ccaaatggaa
     2401 ccgtcattta cgagctcagg aaaaaggaga tacccgtctt tgggaagtgg cggtactctt
     2461 tcacctccgg gatgcttttc gttccggaga tgtctggctc gctcattcgc gccgctatgg
     2521 tgacctcaag caggtactgg tgccgatgat cgcggcgcag gaaaatgcaa aactggccgt
     2581 gccttccaac ccacaggatt ggctggcaga cagaaaggcg cgactcacga tcgctcttaa
     2641 gcggctggcc cgggctgccc gtaacggcac tattccgcac ggtagcatag aagatggaac
     2701 gttgcggatc gacaggttga cagcagacgt gccggatggt gccgaggcac tcatactgga
     2761 tctgtatcgc cgaatgccgt ccgttcggat taccgacatg ctgcttgaag ttgatgcagc
     2821 ccttggtttc acagatgcgt ttacccatct gagaaccggg gctccatgtc gcgaccggat
     2881 cggtctgctc aacgtcctgc tcgctgaagg gctcaatctg ggcctgcgta agatggcgga
     2941 agctacaaac acgcatgatt actggcagct ctcacgcctt gcccgctggc atgttgaaag
     3001 cgaagccatg aaccaggcat tggcaattgt ggtggccgcg cagggtaaac tgccgatgtc
     3061 acgcgtctgg gggatgggca cgtcagcatc gagcgatggt cagtttttcc cgacagcgcg
     3121 gcatggcgaa gccatgaaca tggtcaatgc caaatatggt tctgttcccg gcctcaaagc
     3181 gtatactcac gtaagcgacc agttcgcgcc attcgcttgt cagtcgatcc cggcgaccgt
     3241 gagcgaggca ccgtatattc tcgatggact actgatgaac gaggtcggtc gccatgttcg
     3301 cgaacagtat gccgatacag caggattcac cgaccatttg ttcggagcca gtagcctgct
     3361 cggctacaat ctcgttctgc gaatcaggga tctgccatcg aagcggttgt acgtatttaa
     3421 tcccgatacg acccccaggg agttacgcaa gttggtaggt ggaaaagccc gggaggatct
     3481 tatcgttgcg aactggcctg atattttccg ttgtgccgcg acgatgaccg ctggcaaaat
     3541 caggcccagc caactcctgc gcaagctcgc ttcttaccca cgacaaaaca accttgcagt
     3601 tgcgcttcgt gaagttggtc gtattgaacg gacccttttc attattgagt ggatcctgga
     3661 tacggacatg cagcggcgtg ctcagatcgg tcttaacaag ggagaggccc accatgcgct
     3721 caaaaatgcg ctccgtatcg ggaggcaggg ggaaattcgc gatcgcacga cagaggggca
     3781 gcactaccga atcgctgggc tcaatttatt gactgcggtg atcatttact ggaataccgt
     3841 ccatcttggt catgccgtca cggagcggcg gaacgaaggg ttggatgttc cccctgaatt
     3901 tcttccccac atatccccat tgggctgggc gcacattcta ctgactggcg aatatctttg
     3961 gcccaaggaa ccgaaagctt agggtgtcat ttcgccctca gccggaaccg acccctagag
     4021 tcaagtcacg agtcgaatcg ccggtgaatc gtgtgctaag tcgtccggtg acttgccata
     4081 acgtgattgc cggccgtcga cgaacacgat tcctgaacgt gctgaaaacc ggttttccgg
     4141 ctgtgccggc aacgcctcac ggccacgcaa taaattgcgt ccccgttcac cgttttcggg
     4201 taagaagaac atcggaaaca ggacttaccc cggctgaatg gcgtgaattt ccgcgctatg
     4261 cacttgcgcg catactcatg catgccgtaa aaacagagcc tgcgcgtttc tggcgggttt
     4321 tcgggtggtt tgttgcctgt tttaccggtt tcccgtcaga aacgcccaga gagcctctca
     4381 ggcggtgctg ataacggatg ttatggtaaa tcgtcaacgc cggcgctgcg gctgccaggt
     4441 gaaggtgtct tccgccgcct catacttctc ccggcgcatt ttcttcgcca gcctggattc
     4501 acgttcgtcc tgaatggcgc gaagcaccct ttcccgcacc tgctgtttta ccgcctttgg
     4561 cagtgattta ttccctttca ggcccatggc cagcgcctgg gcgacaaagg cgtccgccag
     4621 acgcttctct tcagcggggg tgagtttcag gtgtgtcatg gtggcctccg ggtgtctctg
     4681 gcttcctgaa cagcttaatt ggtagtcctc tcctcattcc agtccgcttt cagatatgca
     4741 tgtacttaca taaaagcaag tgtgatgaat atcgcaaata atgaaatttg ttgccggggg
     4801 aaactttcgg gaaaagacgc aacctgctga caggcggctc tggccgcttt tttgtgcctg
     4861 aagatgagaa ctaatctcat atattgacac ctgaaaacag caagggtaag gtaataatta
     4921 tactgttcat atatacagta tattggtttt aatttattgt tttaaatgtc aaagaggata
     4981 acgatatgag tggcggagac ggacgaggtc cgggtaattc aggtctggga cacaatggtg
     5041 gtcaggccag tggaaatgtg aacggtacgt caggtaaagg tggcccttca tctggtggtg
     5101 gtacggatcc aaacagcggg ccgggctggg gtacgacgca tacacctaac ggggatattc
     5161 ataactataa cccgggtgag tttggtaacg gcgggagtaa acccggtggt aatggcggta
     5221 acagcggcaa tcatagcggg agctctggtg gcggacagtc ttcagccacc gcgatggcct
     5281 tcggtctgcc agcccttgcc actccggggg ctgagggact ggctttatcc gtctctggcg
     5341 atgcgttgtc ggccgctgtt gctgatgtgc tggccgccct gaaaggtccg tttaagtttg
     5401 gtctgtgggg gatagcgatc tacggtgtgc tgccttctga gattgcaaaa gatgatccga
     5461 acatgatgtc aaaaattatg acgtcattac cggccgatac ggtgacggag actccggtaa
     5521 gtactttacc actggagcag gcgacggttc gtgtcagaca acgggttgtg gatgtggtga
     5581 aggatgagcg gcagcatatt gcggttgtca cgggtcggcc aatgagtgtt cctgtggtgg
     5641 atgcgaaacc gacaaaacgt ccgggggtat tcagtgtgtc gattccgggt ctcccgtctc
     5701 tgcaggtgag cgtaccgaaa ggtgttccgg ccgcgaaagc cccgccaaaa ggcattgttg
     5761 ctgaaaaagg tgattcacgt ccggctggtt ttacggccgg tggtaactcc cgtgaggccg
     5821 ttattcgttt cccgaaagag accggacaga agccggttta tgtgtcggtg acagatgttc
     5881 ttaccccggc acaggtaaag cagcgtcagg aggaagaaaa gcgtcgtcag caggcatggg
     5941 acgccgctca cccggaagag gggctgaaaa gagagtatga taaagcgaaa gctgacctgg
     6001 atgccgaaga taaaaatatt gcgaccttaa acggtaggat tacatcgaca gaaaaagcca
     6061 ttcctggtgc aagaactgct gttcaggaag ccgataaaaa ggtgaaagaa gccgaggcaa
     6121 ataaagatga ttttgtgact tacaacccgc ctcatgaata tggatccggg tggcaggacc
     6181 aggttcgcta tcttgataag gatattcaga atcagaatga gaaattaaaa gcagctcagg
     6241 catctttaaa cgcaatgaat gaatccttat ccagagataa ggctgcgctt tccggggcga
     6301 tggagacccg gaaacaaaag gagaaaaaag cgaaggatgc agaaaataaa ttaaatgagg
     6361 aaaaggaaaa atctcgcaag ggaactaaag attacggcca tgattatcat ccagccccga
     6421 aaactgaaga cataaagggg ctaggtgacc tcaaaaaagg tacacctaaa acaccaatgc
     6481 agggaggtgg aggtaggcgt aaacgctgga ttggtgataa aggtcgtaag atttatgaat
     6541 gggattccca gcacggtgag cttgaagggt accgcgccag tgatggcgaa cacctcggcg
     6601 cattcgatcc aaaaacgggt aagcaggtta aagggccgga tccaaaaaga aacattaaaa
     6661 aatatctttg agaggtaagt atgggactta aattagattt aacctggttt gataagaaaa
     6721 ctgaagagtt taaaggggaa gagtattcta aagactttgg tgatgatggt tctgtcattg
     6781 aaagtctggg gatgccttta aaggataata ttaacaatgg ttgttttgat gtgaaaaatg
     6841 agtgggtttc attattgcaa ccctacttta aacataaaat taatctttct gatagttcat
     6901 attttgtttc atttgattat cgggatggca actggtaata aaaatttatc aaggatgagt
     6961 tgaaatacag acttccggta ttcaaggatg aatgctggag tctttaaata caaggaaagt
     7021 atatgaaaaa ggtaaaggct atttttcttt ttatattgat agtttctggc tttcttattg
     7081 tcgcatgtca ggcaaactat atcagggatg ttcagggcgg gacggtggca ccatcatcct
     7141 cctctgagct gacgggaatc gcggttcagt agaaaagatc aaaggatctt cttgagatcc
     7201 tttttttctg cgcgtaatct gctgcctgta aacgaaaaaa ccacccggca aggtggtttt
     7261 tcgaaggttc agcaaaccgg caagtttctg aaccgtggta acagtcttgt gcgagacatg
     7321 tcaccaaatc tgtccttcca gtgtagccgc agtcaggcca ccacttcaag aactcttcga
     7381 aacatatctc gcacatttca gttaccagtg gctgctgcca gtggcgattt atcgtgtctt
     7441 tccgggttgg acccaagaga acagttaccg gaacaggcgc agcagtaggg ctgaaagggg
     7501 ggttcctgca tacagcccgg cccggagcga actgcctaag ttggtccgag ataccagaag
     7561 acgtgagcta tgagaaagcg ccacgtgtcc cgtaagggag aaaggcggaa aggtatccgg
     7621 taaccgatat ggcgggaacg tgggggcgca ggagggagcg attcaccgga aacggtgatg
     7681 atctttaagc cacgtcgggt ttcgtcaccg ctggcggatt catggttgag ccttacggct
     7741 cccacagatg cacaaaaaaa gtgtctgtta tgtgaactcc gtcagggggg cggagcctat
     7801 ggaaaaagcg cctgcggcgc ggccttttcg tcctttcttt ttttgcgcac atgttatgtt
     7861 ctgtgatgcg ttatcccctg attctgtgga taaggtatga gtctgttgtg tgagcgtagg
     7921 caacgcccgc cgcagccgaa cggccggagc gtgagcgacc gagtgagcga ggaggcgtca
     7981 tttccccgga accggcattc agcacttacg cgttccggcc gcatttttct cctgccacat
     8041 gaagcacttc cctggtaacc gcactcatgc aaacatacca agcaagccag tatacactcc
     8101 gctagtgcta cgtgactggt tcagggctgc gccccgaaac ccgctaacaa cctcttagct
     8161 actggatatc accccatgcc atctgaatat gatgaacaac gggatgctac tgatacgcag
     8221 cttcccggcg aatatgacct ttttgattta atggcgcagg ttactgaaga gaacctgcat
     8281 gcccctgtgg attctgggcc gcctgtcggc cgggaagcgt tatagctttt tcgcttgccc
     8341 gcgtacgtgg taatacgccg gtattacatt ttatttattt attttcatgt agttacaggt
     8401 gattgtaata cgttgcgtat tacataaata aacggagatt tcagttttta ccttttaatt
     8461 catgcagttg cgatgaatat cgcggctgcg ttaggtgtat gaccatttaa ggggcaaaaa
     8521 attatcattc tggctgcgtg atctgcgcag gttaaggagc atcggcgacg aaaaagcgtc
     8581 tttaactgct tcgttcatgc ttgcatgaac ggatgatttt tccagactac aggtaaacta
     8641 caggaagact acaaaagttg atgatttaat cctgattttt agtgcgatga ctacagataa
     8701 actacaaaat aactacagga ttacctcaga tccgttctct gaaccattcg tacatccgac
     8761 ccccttaccg gtttacactg agcactgatg ctctgagggc ccttatgacg aaacaacaga
     8821 aaacagcgct gaacatggct aaattcatcc agaatcagtc tctgctcctg ctggagaagc
     8881 ttaacgagct tgatctcgat gttgaggcgg acctttgtga gaaactccac gatgatgcgg
     8941 agcatctttt ccgtacactg tcctcgagac tgaatgcatc ccaggatggt aactgatggc
     9001 cagagtatct attagtgagg ctgccagact gacgggtaag agtagaacaa cgctgcacag
     9061 gttaattaaa gcaggtgact tgtccacctg tagcggtgaa cgtaatgcaa aaatggtaga
     9121 tacttcagag cttttgcgtg tcttcggtcc ctttgagcaa cctaagggtg aacacctttc
     9181 tggacaggtc tctgaacagc gtgatacagc ctctgctgac cagaatgaac aggtgattcg
     9241 tcatctgaaa caggagattg aacacctgaa caccttactt ttgtccaagg attcgcacat
     9301 tgacagcctg aaacaggcca tgctccttct tgaaaatcac caggcaaaat taactgaatc
     9361 cccggcgcca tggtggaaat tctggaagaa a
//
